Comprehensive disease-resistant and stress-resistant cultivation system and method for plateau rainy and hot same-season pea germplasm

Abstract

The invention relates to the technical field of planting, and particularly discloses a comprehensive disease-resistant and stress-resistant cultivation system and method for plateau rainy and hot same-season pea germplasm, and the system comprises a pea data collection module which collects the growth and development data of each sample plant of each pea germplasm; the growth cycle matching module is used for matching to obtain the growth cycle of each pea germplasm; the comprehensive performance analysis module is used for collecting cultivation data of each sample plant of each pea germplasm in a preset growth period, and comprehensively analyzing to obtain a pea comprehensive performance evaluation value of each pea germplasm; and the pea performance evaluation module is used for evaluating the pea performance according to the pea comprehensive performance evaluation value of each pea germplasm. According to the comprehensive disease-resistant and stress-resistant cultivation system and method for the plateau rainy and hot same-season pea germplasm, the pea germplasm with potential can be rapidly identified, the requirement of the market for high-quality peas can be met, a scientific planting decision basis is provided for peasants, and the stability and sustainability of pea production are ensured.

Description

Technical Field

[0001] The invention relates to the field of planting technology, and in particular to a comprehensive disease-resistant and stress-resistant cultivation system and method for pea germplasm in the same rainy and hot season on the plateau. Background Art

[0002] At present, the comprehensive disease and stress resistance breeding of plateau rain and heat season pea germplasm is a very important part of the field of planting technology. With the advancement of technology and the growth of demand, improving a more refined comprehensive disease and stress resistance breeding system for plateau rain and heat season pea germplasm has become the norm. Efficient and accurate comprehensive disease and stress resistance breeding method for plateau rain and heat season pea germplasm is of great significance to improving the disease and stress resistance and yield quality of peas.

[0003] For example, the invention patent with the announcement number CN116472956B is a parent combination for screening pea umbel plants and its application, which belongs to the field of plant breeding technology. The pea plants bred by the method of the present invention have a plant height of 82-86 cm, a pod height of 40-44 cm, an effective pod length of 41-43 cm, 2 branches, 9-11 pod layers, 28-32 pods per plant, pod length of 8.9-9.3 cm, 7-9 grains per pod, 100-grain weight of 22-23 g, dark green seeds, wrinkled, and no pod explosion, that is, the target plant pea umbel plant is successfully screened. Moreover, only the parent combination of the present invention can screen out the target plant, and other parent combinations cannot screen out.

[0004] For example, the invention patent with the announcement number CN118696814A is a method for hybrid breeding of peas in high-cold areas, which belongs to the field of pea breeding technology; it includes step one, pollen preservation; step two, emasculation; step three, hybridization; step four, seed treatment; step five, repeated hybridization; step six, cultivation. The present invention uses sodium hypochlorite solution during pollination to reduce the impact of microorganisms on pollination. The plant extract contains a variety of plant active ingredients that can increase the activity of holly pollen and improve the success rate of pollination. The use of buffer solution can protect the activity of pollen and avoid it from being affected by environmental factors such as light, and can also improve the success rate of hybridization in high-cold environments. Iterative repeated hybridization treatment is conducive to peas to obtain stable and excellent genetic traits, further improve the cold resistance of pea plants, and ensure the stability of genetic characteristics in subsequent planting.

[0005] However, in the process of implementing the technical solution of the invention in the embodiments of the present application, the present application discovered that the above technology has at least the following technical problems: the current comprehensive disease-resistant and stress-resistant breeding system for plateau rain-heat season pea germplasm focuses more on the genetic stability of peas, and the quality of peas is difficult to guarantee. Summary of the invention

[0006] In view of the deficiencies in the prior art, the present invention provides a comprehensive disease-resistant and stress-resistant breeding system and method for plateau rain-heat season pea germplasm, which can effectively solve the problems involved in the above-mentioned background technology.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: The first aspect of the present invention provides a comprehensive disease and stress resistance breeding system for plateau rain and heat season pea germplasm, including: a pea data acquisition module, used to obtain plateau rain and heat season pea germplasm, mark it as each pea germplasm, and select sample plants from each pea germplasm for cultivation, and collect growth and development data of each sample plant of each pea germplasm.

[0008] The growth cycle matching module is used to process the growth and development data of each sample plant of each pea germplasm to obtain the growth adaptability evaluation index of each pea germplasm, and to match the growth cycle of each pea germplasm according to the growth adaptability evaluation index of each pea germplasm.

[0009] The comprehensive performance analysis module is used to collect the cultivation data of each sample plant of each pea germplasm during the preset growth cycle, including disease resistance data and stress resistance data, and process the disease resistance data to obtain the pea disease resistance evaluation index of each pea germplasm, and process the stress resistance data to obtain the pea stress resistance evaluation index of each pea germplasm. According to the growth adaptability evaluation index, pea disease resistance evaluation index and pea stress resistance evaluation index of each pea germplasm, a comprehensive analysis is performed to obtain the comprehensive performance evaluation value of peas for each pea germplasm.

[0010] The pea performance evaluation module is used to evaluate the pea performance according to the comprehensive performance evaluation value of each pea germplasm, obtain the evaluation results and provide feedback.

[0011] As a further method, the growth and development data of each sample plant of each pea germplasm is processed to obtain the growth adaptability evaluation index of each pea germplasm. The specific processing process is: the growth and development data includes average leaf area, plant height and number of branches.

[0012] The critical average leaf area, critical plant height and critical branch number were extracted from the pea germplasm database, and the growth adaptability evaluation index of each pea germplasm was obtained through comprehensive analysis.

[0013] As a further method, the growth cycle of each pea germplasm is obtained by matching the growth adaptability evaluation index of each pea germplasm. The specific matching process is: the growth adaptability evaluation index of each pea germplasm is input into the pea germplasm database to match the growth cycle of each pea germplasm corresponding to the growth adaptability evaluation index interval of each pea germplasm, and the growth cycle is the required time for peas to mature.

[0014] As a further method, the disease resistance data is processed to obtain the pea disease resistance evaluation index of each pea germplasm, and the specific processing process is: the disease resistance data includes the incidence rate, leaf death rate and average disease resistance enzyme activity.

[0015] The critical incidence rate, critical leaf dieback rate and critical average disease resistance enzyme activity were extracted from the pea germplasm database, and the pea disease resistance evaluation index of each pea germplasm was obtained by comprehensive analysis.

[0016] As a further method, the stress resistance data is processed to obtain the pea stress resistance evaluation index of each pea germplasm, and the specific processing process is: the stress resistance data includes germination rate, chlorophyll content and malondialdehyde content.

[0017] The critical germination rate, critical chlorophyll content and critical malondialdehyde content were extracted from the pea germplasm database, and the pea stress resistance evaluation index of each pea germplasm was obtained through comprehensive analysis.

[0018] As a further method, the comprehensive analysis obtains the comprehensive performance evaluation value of peas for each pea germplasm. The specific analysis process is: according to the growth adaptability evaluation index, pea disease resistance evaluation index and pea stress resistance evaluation index of each pea germplasm, the comprehensive performance evaluation value of peas for each pea germplasm is obtained by comprehensive analysis. The comprehensive performance evaluation value of peas for each pea germplasm is used to quantify the comprehensive performance of each pea germplasm.

[0019] As a further method, the pea performance is evaluated according to the pea comprehensive performance evaluation value of each pea germplasm. The specific evaluation process is: extracting the pea comprehensive performance evaluation threshold from the pea germplasm database, comparing the pea comprehensive performance evaluation value of each pea germplasm with the pea comprehensive performance evaluation threshold, if the pea comprehensive performance evaluation value of a certain pea germplasm is greater than or equal to the pea comprehensive performance evaluation threshold, then the pea germplasm is evaluated as qualified; if the pea comprehensive performance evaluation value of a certain pea germplasm is less than the pea comprehensive performance evaluation threshold, then the pea germplasm is evaluated as unqualified, and the number of qualified pea germplasm is counted.

[0020] If the number of qualified pea germplasm is zero, the pea germplasm is cultivated again. If the number of qualified pea germplasm is not zero, the best pea germplasm is screened.

[0021] As a further method, the screening obtains the best pea germplasm, and the specific screening process is: sorting the pea comprehensive performance evaluation values ​​of each qualified pea germplasm from large to small, and marking the pea germplasm with the largest pea comprehensive performance evaluation value as the best pea germplasm.

[0022] As a further method, the pea disease resistance evaluation index of each pea germplasm is specifically expressed as:

[0023]

[0024] Among them, Dr. i represents the pea disease resistance evaluation index of the i-th pea germplasm, d i represents the incidence of the ith pea germplasm, d0 represents the critical incidence, e represents the natural constant, K ij represents the leaf death rate of the jth sample plant of the i-th pea germplasm, K0 represents the critical leaf death rate, M ij represents the average disease-resistant enzyme activity of the jth sample plant of the i-th pea germplasm, M0 represents the critical average disease-resistant enzyme activity, ρ1 represents the pea disease resistance evaluation influencing factor corresponding to the set incidence rate, ρ2 represents the pea disease resistance evaluation influencing factor corresponding to the set leaf mortality rate, ρ3 represents the pea disease resistance evaluation influencing factor corresponding to the set average disease-resistant enzyme activity, i represents the number of each pea germplasm, i=1,2,3,...,m, m represents the total number of pea germplasm, j represents the number of each sample plant, j=1,2,3,...,n, n represents the total number of sample plants.

[0025] The second aspect of the present invention provides a comprehensive disease-resistant and stress-resistant breeding method for plateau rain-heat season pea germplasm, comprising: obtaining plateau rain-heat season pea germplasm, marking them as various pea germplasms, selecting sample plants from each pea germplasm for cultivation, and collecting growth and development data of each sample plant of each pea germplasm.

[0026] The growth and development data of each sample plant of each pea germplasm are processed to obtain the growth adaptability evaluation index of each pea germplasm, and the growth cycle of each pea germplasm is obtained according to the growth adaptability evaluation index of each pea germplasm.

[0027] The cultivation data of the preset growth cycle of each sample plant of each pea germplasm is collected, including disease resistance data and stress resistance data. The disease resistance data is processed to obtain the pea disease resistance evaluation index of each pea germplasm, and the stress resistance data is processed to obtain the pea stress resistance evaluation index of each pea germplasm. According to the growth adaptability evaluation index, pea disease resistance evaluation index and pea stress resistance evaluation index of each pea germplasm, a comprehensive analysis is performed to obtain the comprehensive pea performance evaluation value of each pea germplasm.

[0028] The pea performance is evaluated according to the comprehensive performance evaluation value of each pea germplasm, and the evaluation results are obtained and fed back.

[0029] Compared with the prior art, the embodiments of the present invention have at least the following advantages or beneficial effects:

[0030] (1) The present invention provides a comprehensive disease-resistant and stress-resistant breeding system and method for plateau rain-heat season pea germplasm, which can quickly identify pea germplasm with potential, thereby shortening the breeding cycle and accelerating the promotion and application of new varieties, which helps to meet the market demand for high-quality peas. At the same time, it can provide farmers with a scientific basis for planting decisions, further reduce production risks, and ensure the stability and sustainability of pea production.

[0031] (2) The present invention can screen out varieties that are more suitable for specific regions and cultivation requirements by evaluating the growth adaptability evaluation index of each pea germplasm, provide strong support for the improvement and renewal of pea varieties, optimize the growth space of pea plants, improve the utilization efficiency of light energy and nutrients, thereby further improving yield and quality to meet market demand.

[0032] (3) The present invention can screen out pea varieties with strong stress resistance by evaluating the pea stress resistance evaluation index of each pea germplasm, which is helpful for breeding pea varieties with different stress resistance characteristics, thereby enriching pea germplasm resources and promoting the diversified development of varieties. It can also take targeted management measures for specific adverse conditions and improve the utilization efficiency of resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] The present invention is further described using the accompanying drawings, but the embodiments in the accompanying drawings do not constitute any limitation to the present invention. A person skilled in the art can obtain other drawings based on the following drawings without creative work.

[0034] Figure 1 It is a schematic diagram of system module connection of the present invention.

[0035] Figure 2 It is a schematic diagram of the method flow of the present invention.

[0036] Figure 3 The figure is a schematic diagram of the functional relationship between the pea comprehensive performance evaluation value and the pea stress resistance evaluation index of the present invention. DETAILED DESCRIPTION

[0037] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0038] Reference Figure 1As shown, the first aspect of the present invention provides a comprehensive disease-resistant and stress-resistant breeding system for plateau rain-heat season pea germplasm, including: a pea data collection module, used to obtain plateau rain-heat season pea germplasm, mark it as each pea germplasm, and select sample plants from each pea germplasm for cultivation, and collect growth and development data of each sample plant of each pea germplasm.

[0039] The growth cycle matching module is used to process the growth and development data of each sample plant of each pea germplasm to obtain the growth adaptability evaluation index of each pea germplasm, and to match the growth cycle of each pea germplasm according to the growth adaptability evaluation index of each pea germplasm.

[0040] The comprehensive performance analysis module is used to collect the cultivation data of each sample plant of each pea germplasm during the preset growth cycle, including disease resistance data and stress resistance data, and process the disease resistance data to obtain the pea disease resistance evaluation index of each pea germplasm, and process the stress resistance data to obtain the pea stress resistance evaluation index of each pea germplasm. According to the growth adaptability evaluation index, pea disease resistance evaluation index and pea stress resistance evaluation index of each pea germplasm, a comprehensive analysis is performed to obtain the comprehensive performance evaluation value of peas for each pea germplasm.

[0041] The pea performance evaluation module is used to evaluate the pea performance according to the comprehensive performance evaluation value of each pea germplasm, obtain the evaluation results and provide feedback.

[0042] Specifically, the growth and development data of each sample plant of each pea germplasm are processed to obtain the growth adaptability evaluation index of each pea germplasm, and the specific processing process is: the growth and development data include average leaf area, plant height and number of branches.

[0043] The critical average leaf area, critical plant height and critical branch number were extracted from the pea germplasm database, and the growth adaptability evaluation index of each pea germplasm was obtained through comprehensive analysis.

[0044] In a specific embodiment, the average leaf area is an important indicator for measuring the photosynthetic capacity of pea plants. A larger leaf area can capture more light energy, thereby improving photosynthetic efficiency and providing more energy and nutrients for the plant. Therefore, by evaluating the average leaf area, the photosynthetic capacity and growth potential of pea germplasm can be indirectly understood. The leaf area of ​​each leaf can be obtained by a leaf area meter, and the leaf area of ​​each leaf can be summed and divided by the total number of leaves to obtain the average leaf area; plant height is an important indicator for measuring the ability of pea plants to utilize growth space. Taller plants can better utilize the light energy in the upper space, thereby improving light energy utilization and yield. Therefore, by evaluating the plant height, the ability of pea germplasm to utilize growth space can be understood, and the plant height can be directly measured by a tape measure; the number of branches is an important indicator for measuring the growth potential and yield potential of pea plants. More branches mean more growth points and pod-forming sites, which may increase yield. Therefore, by evaluating the number of branches, the growth potential and yield potential of pea germplasm can be understood, and the number of branches can be obtained by statistics.

[0045] Furthermore, the growth adaptability evaluation index of each pea germplasm is expressed as follows:

[0046]

[0047] Among them, Ga i represents the growth adaptability evaluation index of the i-th pea germplasm, e represents the natural constant, S ij represents the average leaf area of ​​the jth sample plant of the i-th pea germplasm, S0 represents the critical average leaf area, and h ij represents the height of the jth sample plant of the i-th pea germplasm, h0 represents the critical plant height, Z ij represents the number of branches of the jth sample plant of the i-th pea germplasm, Z0 represents the critical number of branches, ω1 represents the growth adaptability assessment influencing factor corresponding to the set average leaf area, ω2 represents the growth adaptability assessment influencing factor corresponding to the set plant height, ω3 represents the growth adaptability assessment influencing factor corresponding to the set number of branches, i represents the number of each pea germplasm, i=1,2,3,...,m, m represents the total number of pea germplasm, j represents the number of each sample plant, j=1,2,3,...,n, n represents the total number of sample plants.

[0048] The algorithm of this embodiment combines the average leaf area, plant height and number of branches of each sample plant of each pea germplasm, and comprehensively analyzes to obtain the growth adaptability evaluation index of each pea germplasm. The height and number of branches of pea plants often show a positive correlation trend. In other words, taller plants often have more branches. This may be because taller plants can use light energy more effectively to promote the growth and development of side branches; there may not be a direct linear relationship between the average leaf area and plant height, but the two may be indirectly related through other factors such as photosynthesis efficiency and nutrient distribution. Taller plants may have a larger leaf area, so that they can capture more light energy for photosynthesis; in some cases, the average leaf area and the number of branches may show a trend of synergistic growth, that is, as the number of branches increases, the average leaf area may also increase accordingly. This may be because the increase in branches promotes the growth and development of leaves. Comprehensive analysis can obtain a more comprehensive, accurate and in-depth growth adaptability evaluation index.

[0049] Table 1 Examples of growth adaptability evaluation index data for various pea germplasms

[0050]

[0051]

[0052] As shown in Table 1, the growth adaptability evaluation index of each pea germplasm is determined by the average leaf area, plant height and number of branches of each sample plant of each pea germplasm. In a specific embodiment, n=1, and the critical average leaf area is 8cm 2, the critical plant height is 2m, the critical number of branches is 10, the growth adaptability assessment impact factor corresponding to the set average leaf area is 0.3, the growth adaptability assessment impact factor corresponding to the set plant height is 0.4, and the growth adaptability assessment impact factor corresponding to the set number of branches is 0.3. This formula takes into account three key factors, namely the average leaf area, plant height and number of branches, which can screen out germplasm with excellent traits, help speed up the breeding process and improve breeding efficiency. It can also optimize and reasonably allocate germplasm resources according to their growth characteristics and yield potential, thereby promoting the sustainable development of the pea industry, and can also formulate more reasonable cultivation and management measures, such as irrigation, fertilization, pruning, etc., to further improve the growth rate and yield of pea plants. By standardizing the average leaf area, plant height and number of branches to ensure that they are compared at the same magnitude, the fairness and comparability of the evaluation are improved. At the same time, the settings of S0, h0 and Z0 help to make more effective use of land resources, water resources and nutrient resources and improve production efficiency. By weighting the impact of average leaf area, plant height and number of branches, their relative importance in the evaluation index is reflected. The weights of different factors can be adjusted according to different needs, making the model highly adaptable. It is not difficult to see that the larger the average leaf area, plant height or number of branches, the larger the growth adaptability evaluation index of each pea germplasm. By evaluating the growth adaptability evaluation index of each pea germplasm, it is possible to screen out varieties that are more suitable for specific regions and cultivation needs, provide strong support for the improvement and renewal of pea varieties, optimize the growth space of pea plants, improve light energy utilization and nutrient utilization efficiency, thereby further improving yield and quality, helping to predict the yield potential of different varieties, and providing farmers and agricultural enterprises with a scientific basis for yield prediction, helping them to formulate more reasonable production plans and sales strategies, and further ensuring that the quality of pea products is stable and reliable to meet market demand.

[0053] It should be understood that, in this embodiment, ω1, ω2 and ω3 are respectively growth adaptability assessment influencing factors corresponding to the average leaf area, plant height and number of branches preset in the pea germplasm database, which represent the numerical values ​​of the degree of influence of the average leaf area, plant height and number of branches on the growth adaptability assessment index of the pea germplasm. When used, they can be directly obtained from the pea germplasm database, and the corresponding relationship can be a preset mapping relationship. For example, the average leaf area, plant height and number of branches of each sample plant of each pea germplasm and the growth adaptability assessment influencing factors corresponding to the average leaf area, plant height and number of branches preset in the pea germplasm database form a mapping set, and the real-time average leaf area, plant height and number of branches are input into the mapping set to obtain the growth adaptability assessment influencing factors corresponding to the average leaf area, plant height and number of branches. The mapping relationship can be one-to-one or many-to-one. In this example, its value range is between 0 and 1.

[0054] It should be explained that the growth adaptability evaluation index of each pea germplasm in this embodiment is a quantitative indicator obtained by analyzing the average leaf area, plant height and number of branches of each sample plant of each pea germplasm, and is used to quantify the growth adaptability of each pea germplasm.

[0055] Furthermore, the growth cycle of each pea germplasm is obtained according to the growth adaptability evaluation index of each pea germplasm. The specific matching process is: the growth adaptability evaluation index of each pea germplasm is input into the pea germplasm database to match the growth cycle of each pea germplasm corresponding to the growth adaptability evaluation index interval of each pea germplasm, and the growth cycle is the required time for peas to mature.

[0056] Specifically, the disease resistance data are processed to obtain the pea disease resistance evaluation index of each pea germplasm, and the specific processing process is: the disease resistance data include the incidence rate, leaf death rate and average disease resistance enzyme activity.

[0057] The critical incidence rate, critical leaf dieback rate and critical average disease resistance enzyme activity were extracted from the pea germplasm database, and the pea disease resistance evaluation index of each pea germplasm was obtained by comprehensive analysis.

[0058] In a specific embodiment, the morbidity rate refers to the ratio of the number of diseased plants to the total number of plants. The morbidity rate is an important indicator for evaluating the health status of pea plants. By monitoring the morbidity rate, early symptoms of diseases can be discovered in time, providing early warning for disease prevention and control. It can be obtained by statistics and calculation. The leaf dieback rate refers to the ratio of the number of dead leaves to the total number of leaves. The leaf dieback rate is an intuitive indicator for evaluating the water status and health status of pea plants. Too many dead leaves usually mean that the plants have suffered from severe drought, disease or malnutrition. By monitoring the leaf dieback rate, field management measures such as irrigation and fertilization can be guided. It can be obtained by statistics and calculation. The average disease-resistant enzyme activity refers to the average value of superoxide dismutase, peroxidase, polyphenol oxidase and phenylalanine ammonia lyase activities. Disease-resistant enzyme activity is an important line of defense for pea plants against disease invasion. By monitoring the disease-resistant enzyme activity, the physiological state of the plant can be understood in time, thereby protecting the integrity of cell structure and function. It can be calculated by (ΔA / min) / ε×V, where ΔA is the change in absorbance per unit time, which can be measured by a spectrophotometer, and ε is the molar absorptivity of the product, which is a constant usually obtained through experimental determination or literature review. V is the reaction volume, which can be measured by a volume measurement device.

[0059] Furthermore, the specific numerical expression of the pea disease resistance evaluation index of each pea germplasm is:

[0060]

[0061] Among them, Dr. i represents the pea disease resistance evaluation index of the i-th pea germplasm, d i represents the incidence of the ith pea germplasm, d0 represents the critical incidence, e represents the natural constant, K ij represents the leaf death rate of the jth sample plant of the i-th pea germplasm, K0 represents the critical leaf death rate, M ij represents the average disease-resistant enzyme activity of the jth sample plant of the i-th pea germplasm, M0 represents the critical average disease-resistant enzyme activity, ρ1 represents the pea disease resistance evaluation influencing factor corresponding to the set incidence rate, ρ2 represents the pea disease resistance evaluation influencing factor corresponding to the set leaf mortality rate, ρ3 represents the pea disease resistance evaluation influencing factor corresponding to the set average disease-resistant enzyme activity, i represents the number of each pea germplasm, i=1,2,3,...,m, m represents the total number of pea germplasm, j represents the number of each sample plant, j=1,2,3,...,n, n represents the total number of sample plants.

[0062] The algorithm of this embodiment combines the incidence rate, leaf death rate and average disease resistance enzyme activity of each pea germplasm, and comprehensively analyzes to obtain the pea disease resistance evaluation index. There is often a certain positive correlation between the incidence rate and the leaf death rate. When the pea germplasm is attacked by the disease, the incidence rate increases, accompanied by an increase in the leaf death rate. This is because the disease will destroy the cell structure of the leaves, causing the leaves to be unable to photosynthesize and absorb nutrients normally, and eventually die; when the pea germplasm is attacked by the disease, the activity of the disease resistance enzyme in the body usually changes, and the activity of some enzymes related to disease resistance may increase to cope with the invasion of the disease, thereby reducing the incidence rate; the level of disease resistance enzyme activity can affect the speed and degree of leaf death rate. If the disease resistance enzyme activity is high, it may be able to protect leaf cells from the invasion of the disease or slow down the development of the disease, thereby reducing the leaf death rate. Comprehensive analysis can obtain a more comprehensive, accurate and in-depth pea disease resistance evaluation index.

[0063] It should be explained that three key factors, namely, the incidence rate, leaf withering rate and average disease-resistant enzyme activity of each pea germplasm, are considered in this embodiment, which can comprehensively evaluate the disease resistance of pea germplasm, provide an important basis for disease-resistant breeding, and predict the occurrence of diseases in different germplasms under specific environments, so as to take corresponding preventive measures, such as reasonable fertilization, scientific irrigation, and strengthening field management, to reduce the probability of disease occurrence. By regulating environmental conditions such as temperature, humidity, light, etc., or using biological pesticides, chemical pesticides and other measures, the activity level of disease-resistant enzymes in peas can be induced or increased, thereby enhancing the resistance of peas to diseases and providing rich genetic resources for disease-resistant breeding. By standardizing the incidence rate, leaf withering rate and average disease-resistant enzyme activity of each pea germplasm, ensuring that they are compared at the same magnitude, the fairness and comparability of the evaluation are improved. At the same time, the setting of d0, K0 and M0 helps to timely monitor and control the spread of pea diseases, so as to take timely measures for prevention and control. By weighting the impact of the incidence rate, leaf withering rate and average disease-resistant enzyme activity of each pea germplasm, their relative importance in the evaluation index can be reflected. The weights of different factors can be adjusted according to different needs, making the formula highly adaptable. It is not difficult to see that the smaller the incidence rate of each pea germplasm, the smaller the leaf withering rate or the greater the average disease-resistant enzyme activity, the greater the pea disease resistance evaluation index of each pea germplasm. By evaluating the pea disease resistance evaluation index of each pea germplasm, it is helpful to screen out germplasms with excellent disease resistance traits, reduce the impact of diseases on pea yield and quality, and more targetedly select parents with strong disease resistance for hybrid breeding, thereby cultivating new varieties with excellent disease resistance. It can also timely discover and promote new varieties with excellent disease resistance, thereby accelerating the renewal and replacement of varieties. At the same time, it can reduce the use of pesticides, thereby reducing the amount of pesticide residues in peas, ensuring food safety and consumer health, and further enhancing market competitiveness.

[0064] It should be understood that in this embodiment, ρ1, ρ2 and ρ3 are pea disease resistance assessment influencing factors corresponding to the incidence rate, leaf death rate and average disease resistance enzyme activity preset in the pea germplasm database, respectively, which represent the numerical values ​​of the degree of influence of the incidence rate, leaf death rate and average disease resistance enzyme activity on the pea disease resistance assessment index. When used, they can be directly obtained from the pea germplasm database, and the corresponding relationship can be a preset mapping relationship. For example, the incidence rate, leaf death rate and average disease resistance enzyme activity of each pea germplasm and the pea disease resistance assessment influencing factors corresponding to the incidence rate, leaf death rate and average disease resistance enzyme activity preset in the pea germplasm database form a mapping set, and the real-time incidence rate, leaf death rate and average disease resistance enzyme activity are input into the mapping set to obtain the pea disease resistance assessment influencing factors corresponding to the incidence rate, leaf death rate and average disease resistance enzyme activity. The mapping relationship can be one-to-one or many-to-one. In this example, its value range is between 0 and 1.

[0065] It should be explained that the pea disease resistance evaluation index of each pea germplasm in this embodiment is a quantitative index obtained by analyzing the incidence rate, leaf withering rate, and average disease resistance enzyme activity of each pea germplasm, and is used to quantify the disease resistance performance of each pea germplasm.

[0066] Specifically, the pea stress resistance evaluation index of each pea germplasm is obtained by processing stress resistance data. The specific processing process is as follows: The stress resistance data includes germination rate, chlorophyll content, and malondialdehyde content.

[0067] Extract the critical germination rate, critical chlorophyll content, and critical malondialdehyde content from the pea germplasm database, and comprehensively analyze to obtain the pea stress resistance evaluation index of each pea germplasm.

[0068] In a specific embodiment, the germination rate refers to the ratio of the number of germinated seeds to the total number of seeds. The germination rate is an important indicator to measure the germination ability of pea seeds and is directly related to the emergence rate and field performance of the seeds. Pea seeds with a high germination rate can ensure neat emergence in the field and increase crop yield, and can be obtained by recording observations and statistical calculations; the chlorophyll content refers to the total chlorophyll content. Chlorophyll is a key pigment for plants to carry out photosynthesis, and its content directly affects the photosynthesis and growth and development of plants. Pea germplasms with a high chlorophyll content usually have stronger photosynthesis ability and growth potential, which is beneficial to improving the yield and quality of crops, and can be calculated by (20.21×A645 + 8.02×A663)×Vext×D÷m÷1000, where A645 and A663 respectively represent the absorbance values at wavelengths of 645 nm and 663 nm, measured by a spectrophotometer; Vext represents the volume of the extraction solution, which can be measured by a container; D represents the dilution factor, which can be directly recorded; m represents the sample mass, which can be obtained by weighing; malondialdehyde is a lipid peroxidation product produced by plant cells under stress, and an increase in its content usually means that the cells are damaged. By measuring the malondialdehyde content, the stress resistance of pea germplasms, such as drought resistance and heat resistance, can be evaluated, and the malondialdehyde content can be calculated by (malondialdehyde concentration × sample volume) / (sample mass × molar mass of malondialdehyde), where the malondialdehyde concentration can be obtained by gas chromatography, the sample volume can be measured by a volumetric measuring device, the sample mass can be measured by a weighing device, and the molar mass of malondialdehyde can be directly obtained.

[0069] Furthermore, the specific numerical expression of the pea stress resistance evaluation index of each pea germplasm is:

[0070]

[0071] Among them, Sr irepresents the pea stress resistance evaluation index of the i-th pea germplasm, L i represents the germination rate of the i-th pea germplasm, L0 represents the critical germination rate, g ij represents the chlorophyll content of the jth sample plant of the i-th pea germplasm, g0 represents the critical chlorophyll content, D ij represents the MDA content of the jth sample plant of the i-th pea germplasm, D0 represents the critical MDA content, Indicates the impact factor of pea stress resistance assessment corresponding to the set germination rate, Indicates the impact factor of pea stress resistance assessment corresponding to the set chlorophyll content, represents the impact factor of pea stress resistance assessment corresponding to the set malondialdehyde content, i represents the number of each pea germplasm, i=1,2,3,...,m, m represents the total number of pea germplasm, j represents the number of each sample plant, j=1,2,3,...,n, n represents the total number of sample plants.

[0072] The algorithm of this embodiment combines the germination rate, chlorophyll content and malondialdehyde content of each pea germplasm, and comprehensively analyzes to obtain the pea stress resistance evaluation index of each pea germplasm. There may be a certain positive correlation between the germination rate of pea seeds and their chlorophyll content. Chlorophyll is a key pigment for photosynthesis in plants. Its content directly affects the photosynthesis and growth and development of plants. Pea seeds with high chlorophyll content may have stronger photosynthesis ability and growth potential, which is beneficial to seed germination and seedling growth; there may be a certain negative correlation between the germination rate of pea seeds and the malondialdehyde content. MDA is a lipid peroxidation product produced by plant cells under adverse stress such as drought, high temperature, low temperature, etc. The increase in its content usually means that the cells are damaged. Under adverse conditions such as drought stress, the MDA content of pea seeds may increase, which may inhibit seed germination and seedling growth, thereby reducing the germination rate; there may be a certain correlation between chlorophyll content and MDA content, but this correlation may vary depending on the stress environment. For example, under drought stress, the chlorophyll content of pea leaves may decrease, while the MDA content may increase. This may be because drought stress causes damage to plant cells, which in turn affects the synthesis and degradation of chlorophyll and promotes the production of lipid peroxidation reactions. However, under other stress environments such as high temperature, low temperature, and salt stress, the correlation between chlorophyll content and MDA content may show different trends. Comprehensive analysis can obtain a more comprehensive, accurate, and in-depth pea stress resistance evaluation index.

[0073] It should be explained that in this embodiment, three key factors, namely, the germination rate, chlorophyll content and malondialdehyde content of each pea germplasm, are considered, which can comprehensively evaluate the quality of pea germplasm, provide a scientific basis for breeding high-yield, high-quality and stress-resistant pea varieties, and formulate targeted planting strategies. For example, for pea germplasm with high germination rate and high chlorophyll content, management measures such as close planting and high fertilizer can be adopted to give full play to its growth potential, and the target gene can be more accurately located to improve breeding efficiency, which is helpful to tap germplasm resources with potential utilization value and provide new material sources for pea breeding. By standardizing the germination rate, chlorophyll content and malondialdehyde content of each pea germplasm, ensuring that they are compared at the same magnitude, the fairness and comparability of the evaluation are improved. At the same time, the setting of L0, g and D0 helps to screen out excellent pea germplasm with high germination rate, high chlorophyll content and low malondialdehyde content, thereby improving yield and quality. By weighting the influence of the germination rate, chlorophyll content and malondialdehyde content of each pea germplasm, their relative importance in the evaluation index can be reflected. The weights of different factors can be adjusted according to different needs, making the formula highly adaptable. It is not difficult to see that the greater the germination rate of each pea germplasm, the greater the chlorophyll content or the smaller the malondialdehyde content, the greater the pea stress resistance evaluation index of each pea germplasm. By evaluating the pea stress resistance evaluation index of each pea germplasm, pea varieties with strong stress resistance can be screened out, which is helpful for breeding pea varieties with different stress resistance characteristics, thereby enriching pea germplasm resources and promoting the diversified development of varieties. Targeted management measures such as irrigation, fertilization, and shading can also be taken for specific adverse conditions to improve the stress resistance and yield of peas, further more effectively respond to natural disasters and reduce the impact of disasters on agricultural production, help farmers use water resources, fertilizers and other resources more effectively, reduce resource waste and pollution, and help promote the green development of agriculture and improve resource utilization efficiency.

[0074] It should be understood that in this embodiment and They are the pea stress resistance assessment influencing factors corresponding to the germination rate, chlorophyll content and malondialdehyde content preset in the pea germplasm database, respectively, which represent the numerical values ​​of the influence degree of germination rate, chlorophyll content and malondialdehyde content on the pea stress resistance assessment index. When used, they can be directly obtained from the pea germplasm database, and the corresponding relationship can be a preset mapping relationship. For example, the germination rate, chlorophyll content and malondialdehyde content of each pea germplasm and the pea stress resistance assessment influencing factors corresponding to the germination rate, chlorophyll content and malondialdehyde content preset in the pea germplasm database form a mapping set, and the real-time germination rate, chlorophyll content and malondialdehyde content are input into the mapping set to obtain the pea stress resistance assessment influencing factors corresponding to the germination rate, chlorophyll content and malondialdehyde content. The mapping relationship can be one-to-one or many-to-one. In this example, the value range is between 0 and 1.

[0075] It should be explained that the pea stress resistance evaluation index of each pea germplasm in this embodiment is a quantitative index obtained by analyzing the germination rate, chlorophyll content and malondialdehyde content of each pea germplasm, and is used to quantify the stress resistance of each pea germplasm.

[0076] Specifically, a comprehensive analysis is performed to obtain a comprehensive pea performance evaluation value for each pea germplasm. The specific analysis process is as follows: based on the growth adaptability evaluation index, the pea disease resistance evaluation index and the pea stress resistance evaluation index of each pea germplasm, a comprehensive analysis is performed to obtain a comprehensive pea performance evaluation value for each pea germplasm. The comprehensive pea performance evaluation value for each pea germplasm is used to quantify the comprehensive performance of each pea germplasm.

[0077] Furthermore, the comprehensive performance evaluation value of peas of each pea germplasm is expressed as follows:

[0078] Cp i =tanh(θ1*Ga i +θ2*Dr i +θ3*Sr i );

[0079] Among them, Cp i represents the comprehensive performance evaluation value of pea for the i-th pea germplasm, Ga i represents the growth adaptability evaluation index of the ith pea germplasm, Dr i represents the pea disease resistance evaluation index of the i-th pea germplasm, Sr i represents the pea stress resistance evaluation index of the i-th pea germplasm, θ1 represents the pea comprehensive performance evaluation influencing factor corresponding to the set growth adaptability evaluation index, θ2 represents the pea comprehensive performance evaluation influencing factor corresponding to the set pea disease resistance evaluation index, θ3 represents the pea comprehensive performance evaluation influencing factor corresponding to the set pea stress resistance evaluation index, i represents the number of each pea germplasm, i=1,2,3,...,m, and m represents the total number of pea germplasm.

[0080] like Figure 3 As shown, in a specific embodiment, m = 1, θ1 = 0.3, θ2 = θ3 = 0.4. i =Dr i =0.1, the functional relationship between the pea comprehensive performance evaluation value and the pea stress resistance evaluation index is shown in curve a; when Ga i =Dr i =0.5, the functional relationship between the pea comprehensive performance evaluation value and the pea stress resistance evaluation index is shown in curve b; when Ga i =Dr i =1, the functional relationship between the pea comprehensive performance evaluation value and the pea stress resistance evaluation index is shown in curve c.

[0081] The algorithm of this embodiment combines the growth adaptability evaluation index, pea disease resistance evaluation index and pea stress resistance evaluation index of each pea germplasm, and comprehensively analyzes to obtain the pea comprehensive performance evaluation value of each pea germplasm. Pea germplasm with strong growth adaptability often has stronger disease resistance. This is because these germplasms can better adapt to the environment and thus show stronger resistance when facing diseases. Therefore, pea varieties with high growth adaptability evaluation indexes often have higher disease resistance evaluation indexes. Similarly, pea germplasm with strong growth adaptability also has stronger stress resistance. These germplasms can maintain a good growth state under adverse conditions such as drought, high temperature and low temperature, thereby ensuring the yield and quality of peas. Therefore, there is also a positive correlation between the growth adaptability evaluation index and the stress resistance evaluation index. In addition, pea germplasm with strong disease resistance often has stronger growth adaptability because these germplasms can better resist the invasion of diseases, thereby maintaining a good growth state and yield. At the same time Pea germplasm with strong disease resistance often shows stronger stress resistance under adverse conditions, because these germplasm has stronger physiological regulation ability and metabolic adaptability, and can maintain good growth and development under adverse conditions. Therefore, there is a certain positive correlation between the disease resistance evaluation index and the stress resistance evaluation index; and pea germplasm with strong stress resistance often has stronger growth adaptability, because these germplasm can maintain a good growth state under adverse conditions, thereby ensuring the yield and quality of peas. Therefore, there is a positive correlation between the stress resistance evaluation index and the growth adaptability evaluation index; although the stress resistance evaluation index and the disease resistance evaluation index are two different evaluation indicators, there is also a certain correlation between them. This is because pea germplasm with strong stress resistance often has stronger physiological regulation ability and metabolic adaptability. These characteristics also help to improve the resistance of peas to diseases. Comprehensive analysis can obtain a more comprehensive, accurate and in-depth evaluation value of the comprehensive performance of peas.

[0082] It should be explained that three key factors are taken into account in this embodiment, namely, the growth adaptability evaluation index of each pea germplasm, the pea disease resistance evaluation index and the pea stress resistance evaluation index. Pea germplasm with strong adaptability, good disease resistance and strong stress resistance can be screened out, so as to better cope with various environmental conditions and disease challenges and improve the yield and quality of peas. In addition, high-quality pea germplasm can reduce the occurrence of diseases and pests, reduce the use of pesticides and fertilizers, further improve planting benefits, and enhance the adaptability of varieties, which helps to reduce the risks of agricultural production, improve farmers' planting confidence, and promote the progress and innovation of pea breeding technology. It can help breeders more accurately select pea germplasm with excellent traits for hybridization and breeding, thereby improving the genetic level of pea varieties, helping to promote the sustainable and healthy development of the pea industry and injecting new vitality and impetus into agricultural production. By weighting the influence of the growth adaptability evaluation index, pea disease resistance evaluation index and pea stress resistance evaluation index of each pea germplasm, their relative importance in the evaluation index can be reflected. The weights of different factors can be adjusted according to different needs, making the formula highly adaptable. It is not difficult to see that the greater the growth adaptability evaluation index, pea disease resistance evaluation index or pea stress resistance evaluation index of each pea germplasm, the greater the pea comprehensive performance evaluation value of each pea germplasm. By evaluating the pea comprehensive performance evaluation value of each pea germplasm, it is possible to quickly identify potential pea germplasms, thereby shortening the breeding cycle, accelerating the promotion and application of new varieties, and selecting pea germplasms with excellent quality, which is helpful to meet the market demand for high-quality peas, promote the upgrading and development of the pea industry, promote the innovation and development of pea breeding technology, and provide technical support for the sustainable development of the pea industry. At the same time, it can provide farmers with a scientific basis for planting decisions, which helps farmers formulate response measures in advance, reduce production risks, and ensure the stability and sustainability of pea production.

[0083] It should be understood that in this embodiment, θ1, θ2 and θ3 are the pea stress resistance assessment influencing factors corresponding to the growth adaptability assessment index, pea disease resistance assessment index and pea stress resistance assessment index preset in the pea germplasm database, respectively, which represent the numerical values ​​of the influence of the growth adaptability assessment index, pea disease resistance assessment index and pea stress resistance assessment index on the pea stress resistance assessment index. When used, they can be directly obtained from the pea germplasm database, and the corresponding relationship can be a preset mapping relationship. For example, the growth adaptability assessment index, pea disease resistance assessment index and pea stress resistance assessment index of each pea germplasm are A mapping set is formed between the pea stress resistance assessment index and the pea stress resistance assessment influencing factors corresponding to the growth adaptability assessment index, pea disease resistance assessment index and pea stress resistance assessment index preset in the pea germplasm database, and the real-time growth adaptability assessment index, pea disease resistance assessment index and pea stress resistance assessment index are input into the mapping set to obtain the pea stress resistance assessment influencing factors corresponding to the growth adaptability assessment index, pea disease resistance assessment index and pea stress resistance assessment index. The mapping relationship can be one-to-one or many-to-one. In this example, the value range is between 0 and 1.

[0084] Specifically, the pea performance is evaluated according to the comprehensive performance evaluation value of each pea germplasm. The specific evaluation process is: extracting the comprehensive performance evaluation threshold of peas from the pea germplasm database, comparing the comprehensive performance evaluation value of peas of each pea germplasm with the comprehensive performance evaluation threshold of peas, if the comprehensive performance evaluation value of peas of a certain pea germplasm is greater than or equal to the comprehensive performance evaluation threshold of peas, then the pea germplasm is evaluated as qualified; if the comprehensive performance evaluation value of peas of a certain pea germplasm is less than the comprehensive performance evaluation threshold of peas, then the pea germplasm is evaluated as unqualified, and the number of qualified pea germplasms is counted.

[0085] If the number of qualified pea germplasm is zero, the pea germplasm is cultivated again. If the number of qualified pea germplasm is not zero, the best pea germplasm is screened.

[0086] It should be explained that the optimal pea germplasm was screened in this embodiment, and the specific screening process was: the pea comprehensive performance evaluation values ​​of each qualified pea germplasm were sorted in order from large to small, and the pea germplasm with the largest pea comprehensive performance evaluation value was marked as the optimal pea germplasm.

[0087] Reference Figure 2 As shown, the second aspect of the present invention provides a comprehensive disease-resistant and stress-resistant breeding method for plateau rain-heat season pea germplasm, including: obtaining plateau rain-heat season pea germplasm, marking them as various pea germplasms, and selecting sample plants from each pea germplasm for cultivation, and collecting growth and development data of each sample plant of each pea germplasm.

[0088] The growth and development data of each sample plant of each pea germplasm are processed to obtain the growth adaptability evaluation index of each pea germplasm, and the growth cycle of each pea germplasm is obtained according to the growth adaptability evaluation index of each pea germplasm.

[0089] The cultivation data of the preset growth cycle of each sample plant of each pea germplasm is collected, including disease resistance data and stress resistance data. The disease resistance data is processed to obtain the pea disease resistance evaluation index of each pea germplasm, and the stress resistance data is processed to obtain the pea stress resistance evaluation index of each pea germplasm. According to the growth adaptability evaluation index, pea disease resistance evaluation index and pea stress resistance evaluation index of each pea germplasm, a comprehensive analysis is performed to obtain the comprehensive pea performance evaluation value of each pea germplasm.

[0090] The pea performance is evaluated according to the comprehensive performance evaluation value of each pea germplasm, and the evaluation results are obtained and fed back.

[0091] The pea germplasm database is used to store pea germplasm-related data, including: critical average leaf area, critical plant height, critical number of branches, critical incidence rate, critical leaf dieback rate, critical average disease-resistant enzyme activity, critical germination rate, critical chlorophyll content, critical malondialdehyde content, pea comprehensive performance evaluation influencing factor corresponding to the set growth adaptability evaluation index, pea comprehensive performance evaluation influencing factor corresponding to the set pea disease resistance evaluation index, pea comprehensive performance evaluation influencing factor corresponding to the set pea stress resistance evaluation index and pea comprehensive performance evaluation threshold and other indicators.

[0092] The above contents are merely examples and explanations of the structure of the present invention. The technicians in this technical field may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the structure of the invention or exceed the scope defined by the present invention, they should all fall within the protection scope of the present invention.

Claims

1. A comprehensive disease-resistant and stress-resistant breeding system for pea germplasm in the same rainy and hot seasons on the plateau, characterized in that: include: The pea data collection module is used to obtain pea germplasms in the same season of rain and heat in the plateau, mark them as various pea germplasms, select sample plants from each pea germplasm for cultivation, and collect the growth and development data of each sample plant of each pea germplasm; A growth cycle matching module is used to process the growth and development data of each sample plant of each pea germplasm to obtain the growth adaptability evaluation index of each pea germplasm, and to match the growth cycle of each pea germplasm according to the growth adaptability evaluation index of each pea germplasm; A comprehensive performance analysis module is used to collect the cultivation data of each sample plant of each pea germplasm during a preset growth cycle, including disease resistance data and stress resistance data, process the disease resistance data to obtain the pea disease resistance evaluation index of each pea germplasm, process the stress resistance data to obtain the pea stress resistance evaluation index of each pea germplasm, and comprehensively analyze the growth adaptability evaluation index, pea disease resistance evaluation index and pea stress resistance evaluation index of each pea germplasm to obtain the pea comprehensive performance evaluation value of each pea germplasm; The pea performance evaluation module is used to evaluate the pea performance according to the comprehensive performance evaluation value of each pea germplasm, obtain the evaluation results and provide feedback.

2. The integrated disease and stress resistance breeding system for plateau rain-heat season pea germplasm according to claim 1 is characterized by: The growth and development data of each sample plant of each pea germplasm are processed to obtain the growth adaptability evaluation index of each pea germplasm, and the specific processing process is: The growth and development data include average leaf area, plant height and number of branches; The critical average leaf area, critical plant height and critical branch number were extracted from the pea germplasm database, and the growth adaptability evaluation index of each pea germplasm was obtained through comprehensive analysis.

3. The integrated disease and stress resistance breeding system for plateau rain-heat season pea germplasm according to claim 2 is characterized by: The growth cycle of each pea germplasm is obtained by matching the growth adaptability evaluation index of each pea germplasm. The specific matching process is: The growth adaptability evaluation index of each pea germplasm is input into the pea germplasm database to match the growth cycle of each pea germplasm corresponding to the growth adaptability evaluation index interval of each pea germplasm, and the growth cycle is the required time for peas to mature.

4. The integrated disease and stress resistance breeding system for plateau rain-heat season pea germplasm according to claim 1 is characterized by: The disease resistance data is processed to obtain the pea disease resistance evaluation index of each pea germplasm, and the specific processing process is: The disease resistance data include disease incidence, leaf dieback rate and average disease resistance enzyme activity; The critical incidence rate, critical leaf dieback rate and critical average disease resistance enzyme activity were extracted from the pea germplasm database, and the pea disease resistance evaluation index of each pea germplasm was obtained by comprehensive analysis.

5. The integrated disease and stress resistance breeding system for plateau rain-heat season pea germplasm according to claim 1 is characterized by: The stress resistance data is processed to obtain the pea stress resistance evaluation index of each pea germplasm, and the specific processing process is: The stress resistance data include germination rate, chlorophyll content and malondialdehyde content; The critical germination rate, critical chlorophyll content and critical malondialdehyde content were extracted from the pea germplasm database, and the pea stress resistance evaluation index of each pea germplasm was obtained through comprehensive analysis.

6. The integrated disease and stress resistance breeding system for plateau rain-heat season pea germplasm according to claim 5 is characterized by: The comprehensive analysis obtains the comprehensive performance evaluation value of peas of each pea germplasm, and the specific analysis process is as follows: According to the growth adaptability evaluation index, pea disease resistance evaluation index and pea stress resistance evaluation index of each pea germplasm, a comprehensive analysis is performed to obtain the pea comprehensive performance evaluation value of each pea germplasm, and the pea comprehensive performance evaluation value of each pea germplasm is used to quantify the comprehensive performance of each pea germplasm.

7. The integrated disease and stress resistance breeding system for plateau rain-heat season pea germplasm according to claim 6 is characterized by: The pea performance is evaluated according to the comprehensive performance evaluation value of each pea germplasm, and the specific evaluation process is as follows: Extracting a pea comprehensive performance evaluation threshold from a pea germplasm database, comparing the pea comprehensive performance evaluation value of each pea germplasm with the pea comprehensive performance evaluation threshold, if the pea comprehensive performance evaluation value of a pea germplasm is greater than or equal to the pea comprehensive performance evaluation threshold, then the pea germplasm is evaluated as qualified, if the pea comprehensive performance evaluation value of a pea germplasm is less than the pea comprehensive performance evaluation threshold, then the pea germplasm is evaluated as unqualified, and the number of qualified pea germplasms is counted; If the number of qualified pea germplasm is zero, the pea germplasm is cultivated again. If the number of qualified pea germplasm is not zero, the best pea germplasm is screened.

8. The integrated disease and stress resistance breeding system for plateau rain-heat season pea germplasm according to claim 7 is characterized by: The screening method obtains the best pea germplasm, and the specific screening process is as follows: The pea comprehensive performance evaluation values ​​of each qualified pea germplasm are sorted in descending order, and the pea germplasm with the largest pea comprehensive performance evaluation value is marked as the best pea germplasm.

9. The integrated disease and stress resistance breeding system for plateau rain-heat season pea germplasm according to claim 4 is characterized by: The specific numerical expression of the pea disease resistance evaluation index of each pea germplasm is: Among them, Dr. i represents the pea disease resistance evaluation index of the i-th pea germplasm, d i represents the incidence of the ith pea germplasm, d0 represents the critical incidence, e represents the natural constant, K ij represents the leaf death rate of the jth sample plant of the i-th pea germplasm, K0 represents the critical leaf death rate, M ij represents the average disease-resistant enzyme activity of the jth sample plant of the i-th pea germplasm, M0 represents the critical average disease-resistant enzyme activity, ρ1 represents the pea disease resistance evaluation influencing factor corresponding to the set incidence rate, ρ2 represents the pea disease resistance evaluation influencing factor corresponding to the set leaf mortality rate, ρ3 represents the pea disease resistance evaluation influencing factor corresponding to the set average disease-resistant enzyme activity, i represents the number of each pea germplasm, i=1,2,3,...,m, m represents the total number of pea germplasm, j represents the number of each sample plant, j=1,2,3,...,n, n represents the total number of sample plants.

10. A method for the comprehensive disease resistance and stress resistance breeding system for highland rain-heat season pea germplasm as described in claims 1-9, characterized in that: include: Obtain pea germplasms from the same season of rain and heat in the plateau, mark them as various pea germplasms, select sample plants from each pea germplasm for cultivation, and collect growth and development data of each sample plant of each pea germplasm; The growth and development data of each sample plant of each pea germplasm are processed to obtain the growth adaptability evaluation index of each pea germplasm, and the growth cycle of each pea germplasm is obtained according to the growth adaptability evaluation index of each pea germplasm; Collecting the cultivation data of each sample plant of each pea germplasm during a preset growth cycle, including disease resistance data and stress resistance data, processing the disease resistance data to obtain a pea disease resistance evaluation index of each pea germplasm, processing the stress resistance data to obtain a pea stress resistance evaluation index of each pea germplasm, and comprehensively analyzing the growth adaptability evaluation index, pea disease resistance evaluation index and pea stress resistance evaluation index of each pea germplasm to obtain a pea comprehensive performance evaluation value of each pea germplasm; The pea performance is evaluated according to the comprehensive performance evaluation value of each pea germplasm, and the evaluation results are obtained and fed back.

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