A dietary fiber composition for enhancing immunity and intestinal health and its application

Abstract

The present invention discloses a dietary fiber composition for enhancing immunity and intestinal health and its application, belonging to the technical field of functional foods. The dietary fiber composition is composed of asparagus powder, licorice powder, loquat powder, avocado powder and seabuckthorn powder. The weight ratio of the loquat powder, avocado powder and seabuckthorn powder is 1-10:1-10:0.5-3. The dietary fiber composition composed of asparagus powder, licorice powder, loquat powder, avocado powder and seabuckthorn powder in the present invention has the effects of enhancing immunity, regulating the intestinal flora function and laxative. Experiments have found that when the weight ratio of the loquat powder, avocado powder and seabuckthorn powder is 1-10:1-10:0.5-3, the dietary fiber composition has a synergistic effect in enhancing immunity.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods. Specifically, it relates to a dietary fiber composition for enhancing immunity and intestinal health and its application. Background Art

[0002] Immunity refers to the sum total of biological effects produced during the recognition and response of the body to "antigenic foreign substances" or "foreign components". Under normal circumstances, it is a specific physiological reaction to maintain the stability of the internal environment, and immunity is a defense mechanism of the body itself. Age, physical fitness, and lifestyle habits are three factors that affect immunity. In addition, seasonal changes, unbalanced nutrition, irregular living schedules, excessive fatigue, and stress, etc., can all affect the function of the human immune system.

[0003] Approximately 70% of the human immune system exists in the intestine, and a large number of immune cells in the body, such as T cells, B cells, NK cells, etc., are concentrated in the intestinal mucosa. The intestine is the first line of defense for immunity. As a place for the digestion and absorption of nutrients, microbial colonization, and the aggregation of immune cells, the intestine promotes the interaction among the three. Normal intestinal flora plays an important role in promoting the development of the immune system, maintaining normal immune function, and synergistically antagonizing the invasion of pathogenic bacteria. Therefore, when the intestinal flora is disrupted, the resistance of the human body is likely to deteriorate.

[0004] CN114982891A discloses a preparation method of a fruit and vegetable enzyme stock solution beverage, which is prepared by fermenting raw materials such as asparagus, loquat, and licorice. This invention can make human cells become more active, significantly accelerate metabolism, and improve human immunity, but does not disclose its improvement effect on intestinal health. CN102138594A discloses a compound sea buckthorn tea preparation with the function of regulating intestinal flora. The compound sea buckthorn tea preparation includes, by weight percentage: 15%-90% of fructooligosaccharide, 0%-40% of isomaltooligosaccharide, 0%-30% of galactooligosaccharide, 1%-60% of sea buckthorn fruit powder, 1%-60% of black tea powder, and 0%-50% of excipients. This invention has the health care function of regulating intestinal flora and is suitable for those with intestinal flora disorders, but does not disclose its efficacy in enhancing immunity.

[0005] In summary, it can be seen that the functions of current functional foods for enhancing immunity and regulating intestinal function are relatively single and cannot take both into account. Therefore, it is very necessary to find a dietary fiber composition that simultaneously enhances immunity and intestinal health. Summary of the Invention

[0006] In order to solve the above technical problems, the present invention provides a dietary fiber composition for enhancing immunity and intestinal health and its application.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a dietary fiber composition for enhancing immunity and intestinal health, which is composed of asparagus powder, licorice powder, loquat powder, avocado powder and seabuckthorn powder.

[0009] Asparagus is rich in nutrients such as amino acids, various minerals and vitamins, and also contains various bioactive components such as polysaccharides, saponins and flavonoids. It has the effects of promoting the proliferation and differentiation of immune cells, regulating the functions of immune cells, and promoting bowel movements.

[0010] Licorice contains various bioactive components such as polysaccharides, glycyrrhizic acid, triterpenoids and flavonoid compounds. It can maintain the normal structure and function of cells, regulate the activity and quantity of immune cells, reduce intestinal inflammation, promote the repair and regeneration of intestinal mucosa, inhibit the growth of harmful intestinal bacteria, and promote the balance of intestinal flora.

[0011] Loquat can improve the phagocytosis and bactericidal ability of immune cells, reduce apoptosis, promote intestinal peristalsis, improve the intestinal environment, reduce the accumulation of harmful substances in the intestine, and reduce the risk of intestinal diseases.

[0012] Avocado is rich in unsaturated fatty acids and bioactive components such as phytosterols, tocopherols and squalene. It can enhance the activity of immune cells, support cell repair and regeneration, protect immune cells from oxidative damage, protect the intestinal barrier and promote the growth of intestinal probiotics.

[0013] Seabuckthorn can reduce cell damage, enhance humoral immunity and cellular immunity, and at the same time improve the intestinal microecological structure and maintain the balance of intestinal flora.

[0014] Experiments have proved that the present invention has the effects of enhancing immunity, regulating the function of intestinal flora and promoting bowel movements through the compounding of asparagus powder, licorice powder, loquat powder, avocado powder and seabuckthorn powder.

[0015] In some embodiments, the weight ratio of the loquat powder, avocado powder and seabuckthorn powder is 1-10:1-10:0.5-3.

[0016] In some embodiments, the raw materials of the dietary fiber composition are, by weight: 5-15 parts of asparagus powder, 3-20 parts of licorice powder, 1-10 parts of loquat powder, 1-10 parts of avocado powder and 0.5-3 parts of seabuckthorn powder.

[0017] Preferably, the raw materials of the dietary fiber composition are, by weight: 5-12 parts of asparagus powder, 5-15 parts of licorice powder, 1-5 parts of loquat powder, 3-8 parts of avocado powder and 1-2 parts of seabuckthorn powder.

[0018] Further preferably, the dietary fiber composition comprises the following raw materials by weight: 10 parts of asparagus powder, 8 parts of licorice powder, 3 parts of loquat powder, 5 parts of avocado powder, and 1 part of sea buckthorn powder.

[0019] In some embodiments, the weight ratio of the loquat powder, avocado powder, and sea buckthorn powder is 1-5:3-8:1-2.

[0020] Preferably, the weight ratio of the loquat powder, avocado powder, and sea buckthorn powder is 3:5:1.

[0021] In a second aspect, the present invention provides a method for preparing the above dietary fiber composition for enhancing immunity and intestinal health, comprising the following steps:

[0022] Mix the asparagus powder, licorice powder, loquat powder, avocado powder, and sea buckthorn powder in the formula amounts and sieve.

[0023] In some embodiments, the sieving is through a 60-80 mesh sieve.

[0024] In a third aspect, the present invention provides the application of the above dietary fiber composition for enhancing immunity and intestinal health in the preparation of functional foods.

[0025] In some embodiments, the functions include at least one of enhancing immunity, regulating intestinal flora function, and laxative effect.

[0026] In a fourth aspect, the present invention provides a composition for enhancing immunity and intestinal health, comprising the above dietary fiber composition and excipients acceptable in functional foods.

[0027] In some embodiments, the composition in the present invention is in the form of granules, powder, blocks, or paste.

[0028] The beneficial effects of the present invention are as follows:

[0029] (1) Experiments have proved that the dietary fiber composition composed of asparagus powder, licorice powder, loquat powder, avocado powder, and sea buckthorn powder in the present invention has the effects of enhancing immunity, regulating intestinal flora function, and laxative effect.

[0030] (2) Experiments have found that when the weight ratio of the loquat powder, avocado powder, and sea buckthorn powder is 1-10:1-10:0.5-3, the dietary fiber composition has a synergistic effect in enhancing immunity.

[0031] (3) The composition of the dietary fiber composition of the present invention is clear, has good safety, and the preparation method is simple, which is suitable for large-scale popularization and application.

[0032] (4) The functional food prepared by using the dietary fiber of the present invention can effectively enhance immunity and intestinal health, has good safety, and can be used for a long time. Detailed Embodiments

[0033] The following description of the embodiments is only used to help understand the method and its core idea of the present invention. It should be noted that for those of ordinary skill in the art in this technical field, without departing from the principle of the present invention, several improvements and modifications can still be made to the present invention, and these improvements and modifications also fall within the protection scope of the claims of the present invention. The following description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but can be applied to a wider range consistent with the principles and novel features disclosed herein.

[0034] When the embodiments give a numerical range, it should be understood that unless otherwise stated in the present invention, both endpoints of each numerical range and any value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention belongs.

[0035] It is worth noting that the raw materials used in the present invention are as shown in Table 1 below.

[0036] Table 1 Raw Material Information

[0037]

[0038] Dietary Fiber Compositions of Examples 1 - 5 and Comparative Examples 1 - 4

[0039] Prepare the dietary fiber compositions of different examples and comparative examples according to Table 2 and the following preparation method.

[0040] Preparation Method: Mix the formula amounts of asparagus powder, licorice powder, loquat powder, avocado powder, and seabuckthorn powder, and pass through an 80 - mesh sieve.

[0041] Table 2 Formulations of Dietary Fiber Compositions for Examples and Comparative Examples (unit: parts by weight)

[0042]

[0043] Remarks:

[0044] 1. The "-" in the table represents not added.

[0045] 2. The "*" in the table represents using white kidney bean powder instead of asparagus powder, where the white kidney bean powder is made by grinding white kidney beans.

[0046] 3. The preparation method of Comparative Example 1 in the table is as follows: Mix the formula amounts of asparagus powder, licorice powder, loquat powder and avocado powder, and pass through an 80-mesh sieve.

[0047] 4. The preparation method of Comparative Example 2 in the table is as follows: Mix the formula amounts of asparagus powder, licorice powder and seabuckthorn powder, and pass through an 80-mesh sieve.

[0048] 5. The preparation method of Comparative Example 4 in the table is as follows: Mix the formula amounts of white kidney bean powder, licorice powder, loquat powder, avocado powder and seabuckthorn powder, and pass through an 80-mesh sieve.

[0049] Experimental Example 1. Evaluation of the efficacy of dietary fiber composition in enhancing immunity

[0050] According to the requirements of the "Methods for Functional Testing and Evaluation of Health Foods (2022 Edition)", experiments were conducted to study the efficacy of dietary fiber composition in enhancing immunity.

[0051] 1. Experimental animals

[0052] SPF-grade ICR mice, weighing 18 - 20 g.

[0053] 2. Test samples

[0054] Dietary fiber compositions prepared in Examples 1 - 5 and Comparative Examples 1 - 4.

[0055] 3. Animal grouping

[0056] Randomly divide the ICR mice into a normal group, Examples 1 - 5 groups and Comparative Examples 1 - 4 groups according to body weight, with 8 mice in each group. The Examples 1 - 5 groups and Comparative Examples 1 - 4 groups were gavaged with the dietary fiber compositions prepared in the corresponding examples or comparative examples at a dose of 50 mg / kg, and the normal group was gavaged with an equal volume of normal saline once a day for 30 consecutive days. Each group had normal diet and drinking water.

[0057] After the last gavage, the mice were fasted for 12 h, sacrificed, and the lymphocyte proliferation ability and NK cell activity were measured.

[0058] 4. Experimental methods

[0059] (1) Determination of the proliferation ability of ConA-induced mouse splenic lymphocytes

[0060] The mice were sacrificed, the spleen was aseptically taken, ground, sieved, and a spleen cell suspension was prepared, and the cell number was adjusted to 3×10 6 cells / mL. Each portion of the spleen cell suspension was added to two wells of a 24-well culture plate, 1 mL per well. In one well, 75 μL of ConA solution (equivalent to 7.5 μg·mL -1 ) was added, and the other well was used as a control. Incubate in 5% CO 2 , at 37℃ CO 2Cultivate in an incubator for 72 h. Four hours before the end of cultivation, gently aspirate 0.7 mL of the supernatant from each well, add 0.7 mL of RPMI1640 culture medium without calf serum, and simultaneously add MTT (5 mg·mL -1 ), 50 μL per well, and continue to cultivate for 4 h. After the cultivation is completed, add 1 mL of acidic isopropanol to each well, pipette and mix well to completely dissolve the purple crystals. Then aliquot into a 96-well culture plate, with 3 parallel wells for each well, and use an enzyme-linked immunosorbent assay (ELISA) reader to measure the optical density value at a wavelength of 570 nm.

[0061] Subtract the optical density value of the well without ConA from the optical density value of the well with ConA to represent the proliferation ability of lymphocytes. The results are shown in Table 3.

[0062] Table 3 Effects of dietary fiber compositions on the proliferation ability of ConA-induced mouse splenic lymphocytes

[0063]

[0064] Note: Different letters in the table represent significant differences in data between groups, P < 0.05.

[0065] The results show that:

[0066] Compared with the normal group, the proliferation ability of lymphocytes in the groups of Examples 1-5 was significantly improved (P < 0.05), indicating that the dietary fiber compositions provided in Examples 1-5 have the ability to enhance the proliferation of mouse splenic lymphocytes.

[0067] By comparing the groups of Comparative Example 1-2 and Example 1, it can be seen that in the technical solutions claimed in the present invention, only the dietary fiber compositions in which loquat powder and avocado powder or sea buckthorn powder are compounded with asparagus powder and licorice powder have a poor ability to enhance the proliferation of splenic lymphocytes, and even have no obvious effect, while the dietary fiber compositions in which loquat powder, avocado powder and sea buckthorn powder are compounded with asparagus powder and licorice powder have a significantly improved proliferation ability of lymphocytes (P < 0.05), showing a synergistic effect.

[0068] (2) NK cell activity assay

[0069] Twenty-four hours before the experiment, passage-culture the target cells (YAC-1 cells), and adjust the cell concentration to 4×10 5 cells / mL. Sacrifice the mice, aseptically take the spleen, prepare a spleen cell suspension as effector cells, and adjust the cell concentration to 2×10 7cells / mL. Take 100 μL each of target cells and effector cells (effector-to-target ratio of 50:1) and add them to a U-bottom 96-well culture plate; add 100 μL each of target cells and culture medium to the natural release wells of target cells, and add 100 μL each of target cells and 1% NP40 to the maximum release wells of target cells; set three parallel wells for each of the above items and incubate them in an incubator at 37°C and 5% CO 2 for 4 h, then centrifuge the 96-well culture plate at 1500 r·min -1 for 5 min, aspirate 100 μL of the supernatant from each well and place it in a flat-bottom 96-well culture plate. At the same time, add 100 μL of LDH substrate solution, react for 3 - 10 min according to the room temperature, and add 30 μL of 1 mol·L -1 HCl to each well. Measure the optical density value (OD) at 490 nm using an enzyme-linked immunosorbent assay reader.

[0070] Calculate the NK cell activity according to the following formula, and the results are shown in Table 4.

[0071] NK cell activity (%) =

[0072] Table 4 Effects of dietary fiber composition on NK cell activity in mice

[0073]

[0074] Note: Different letters in the table represent significant differences in data between groups, P < 0.05.

[0075] The results show that:

[0076] Compared with the normal group, the NK cell activity of mice in Example 1 - 5 groups was significantly increased (P < 0.05), indicating that the dietary fiber compositions prepared in Example 1 - 5 have the ability to enhance NK cell activity.

[0077] In summary, the dietary fiber compositions prepared in Example 1 - 5 of the present invention can improve the proliferation ability of lymphocytes, enhance NK cell activity, and have the effect of enhancing immunity.

[0078] Test Example 2: Evaluation of the efficacy of dietary fiber composition in regulating intestinal flora function

[0079] According to the requirements of the "Functional Test and Evaluation Method for Health Foods (2022 Edition)", an experiment was conducted to study the efficacy of the dietary fiber composition in regulating intestinal flora function.

[0080] 1. Experimental animals

[0081] SPF-grade Kunming mice, weighing 18 - 20 g.

[0082] 2. Test samples

[0083] Dietary fiber compositions prepared in Examples 1-5 and Comparative Examples 1-4.

[0084] 3. Animal grouping

[0085] Kunming mice were randomly divided into a normal group, Examples 1-5 groups, and Comparative Examples 1-4 groups according to body weight, with 8 mice in each group. The mice in Examples 1-5 groups and Comparative Examples 1-4 groups were intragastrically administered the dietary fiber compositions prepared in the corresponding examples or comparative examples at a dosage of 50 mg / kg, and the normal group was intragastrically administered an equal volume of normal saline once a day for 14 consecutive days.

[0086] 4. Experimental method

[0087] Before administration, 1.0 g of feces from each group of mice was aseptically collected, and appropriate dilutions were selected and inoculated on each culture medium. After cultivation, the colonies were identified and counted by Gram staining microscopy, and the number of bacteria per gram of wet feces was calculated. After taking the logarithm, statistical processing was performed. 12 h after the last administration, the number of bacteria in the feces of each group of mice was detected again in the same way.

[0088] Table 5 Effects of dietary fiber compositions on intestinal flora in mice

[0089]

[0090] The results showed that:

[0091] Compared with before administration, the number of Bifidobacterium in the feces of mice in Examples 1-5 groups increased significantly (P < 0.05), while the number of Bacteroides decreased significantly (P < 0.05). It indicates that the dietary fiber compositions prepared in Examples 1-5 of the present invention have the function of regulating intestinal flora.

[0092] It should be noted that: compared with before administration, there were no significant changes in the number of Bifidobacterium and Bacteroides in the feces of mice in Comparative Example 2 group (P > 0.05), indicating that the dietary fiber composition prepared in Comparative Example 2 of the present invention does not have the function of regulating intestinal flora.

[0093] Test Example III. Evaluation of the laxative effect of dietary fiber compositions

[0094] 1. Experimental animals

[0095] SPF-grade ICR mice, weighing 18-20 g.

[0096] 2. Test samples

[0097] Dietary fiber compositions prepared in Examples 1-5 and Comparative Examples 1-4.

[0098] 3. Experimental method

[0099] After 1 week of adaptive feeding of ICR mice, they were randomly divided into a normal group, a model group, Example 1-5 groups, and Comparative Example 1-4 groups according to body weight, with 8 mice in each group.

[0100] The Example 1-5 groups and the Comparative Example 1-4 groups were respectively gavaged with the corresponding dietary fiber compositions of the examples and comparative examples at a gavage dose of 50 mg / kg. The normal group and the model group were gavaged with an equal volume of normal saline once a day for 14 consecutive days.

[0101] During the experiment, the mice had free access to water and food. On the 7th and 14th days, loperamide hydrochloride at 5 mg / kg was gavaged to induce constipation in the model mice, and the normal group was gavaged with an equal volume of normal saline.

[0102] After the last gavage, the rats were fasted but allowed to drink water for 24 h. Then, the rats were gavaged with 1 mL / mouse of ink. After 0.5 h, the abdomen was opened to take the small intestine (intestinal tissue from the upper end at the pylorus to the lower end at the ileocecal junction), and the total length (cm) and the ink propulsion length (cm) of the small intestine of the rats were measured. The small intestine ink propulsion rate of the rats was calculated according to the following formula.

[0103] Small intestine ink propulsion rate = ink propulsion length / total length of small intestine × 100%.

[0104] The results are shown in Table 6.

[0105] Table 6 Effects of dietary fiber compositions on moistening the intestine and relieving constipation

[0106]

[0107] Note: Different letters in the table represent significant differences in data between groups, P < 0.05.

[0108] The results showed that:

[0109] Compared with the normal group, the intestinal propulsion rate of the mice in the model group was significantly decreased (P < 0.05), indicating that the mouse model was successfully established.

[0110] Except for the Comparative Example 2 group, compared with the model group, the intestinal propulsion rates of the mice in each group were significantly increased (P < 0.05), indicating that the dietary fiber compositions prepared in Examples 1-5 and Comparative Examples 1, 3, and 4 could all promote intestinal peristalsis and had the effect of moistening the intestine and relieving constipation, while the dietary fiber composition prepared in Comparative Example 2 did not have the effect of moistening the intestine and relieving constipation.

[0111] The above is a further description of the present invention in combination with specific embodiments, but these embodiments are merely exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

Claims

1. A dietary fiber composition for enhancing immunity and intestinal health, characterized in that: The invention is composed of the following raw materials by weight: 5-15 parts of asparagus powder, 3-20 parts of licorice powder, 1-10 parts of loquat powder, 1-10 parts of avocado powder and 0.5-3 parts of sea buckthorn powder.

2. The dietary fiber composition according to claim 1, characterized in that The raw materials are as follows by weight: 5-12 parts of asparagus powder, 5-15 parts of licorice powder, 1-5 parts of loquat powder, 3-8 parts of avocado powder and 1-2 parts of sea buckthorn powder.

3. The dietary fiber composition according to claim 1, characterized in that The raw materials are as follows by weight: 10 parts of asparagus powder, 8 parts of licorice powder, 3 parts of loquat powder, 5 parts of avocado powder and 1 part of sea buckthorn powder.

4. The dietary fiber composition according to claim 1, characterized in that The weight ratio of the loquat powder, avocado powder and sea buckthorn powder is 1-5:3-8:1-2.

5. The dietary fiber composition according to claim 4, characterized in that The weight ratio of the loquat powder, avocado powder and sea buckthorn powder is 3:5:

1.

6. The dietary fiber composition according to any one of claims 1 to 5, characterized in that The method for preparing the dietary fiber composition comprises the following steps: mixing asparagus powder, licorice powder, loquat powder, avocado powder and sea buckthorn powder in a prescribed amount and sieving.

7. Use of the dietary fiber composition according to any one of claims 1 to 6 in the preparation of functional foods, wherein the function is at least one of enhancing immunity, regulating intestinal flora function and moistening the intestine and promoting bowel movements.

8. The use according to claim 7, characterized in that: The functional food includes acceptable auxiliary materials in functional foods.

9. The use according to claim 7, characterized in that: The functional food is in the form of granules, powder, blocks or paste.

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