Tightening and anti-wrinkle composition as well as preparation method and application thereof

Abstract

The invention discloses a firming and anti-wrinkle composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The firming and anti-wrinkle composition provided by the invention is prepared from the following components in parts by mass: 0.0001 to 0.005 part of platinum powder, 0.0001 to 18 parts of palmitoyl pentapeptide-4, 0.0001 to 10 parts of carnosine, 0.0001 to 1 part of a brassica juncea extract and 0.0001 to 3 parts of a guava leaf extract. According to the firming and anti-wrinkle composition provided by the invention, the components in a specific mass part range are selected, and the components have good interaction, so that the firming and anti-wrinkle composition can be used for effectively firming skin, increasing skin elasticity and reducing skin wrinkles when being applied to cosmetics, and also has an excellent moisturizing effect and an excellent skin color brightening effect. Meanwhile, the preparation method of the firming and anti-wrinkle composition provided by the invention is simple to operate, wide in raw material source and beneficial to actual production.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a firming and anti-wrinkle composition and a preparation method and application thereof. Background Art

[0002] With the increase of age and the gradual deterioration of the environment, people's skin will gradually become loose and wrinkles will increase; at the same time, the collagen in facial tissue will decrease, causing the skin to lose elasticity and produce wrinkles, and the moisture content of the skin will also be greatly reduced.

[0003] Nowadays, people have a higher pursuit of firming and anti-wrinkle. Although there are many products with firming and anti-wrinkle effects in the existing technology, in order to meet people's increasing demands, the efficacy of cosmetics needs to be further improved. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a firming and anti-wrinkle composition which can effectively remove wrinkles, tighten the skin, increase skin elasticity, and also has good moisturizing effect and skin gloss enhancement effect, as well as a preparation method and application thereof.

[0005] To achieve the above objectives, in a first aspect of the present invention, the present invention provides a firming and anti-wrinkle composition, which comprises the following components in parts by weight: 0.0001-0.005 parts of platinum powder, 0.0001-18 parts of palmitoyl pentapeptide-4, 0.0001-10 parts of carnosine, 0.0001-1 parts of Balkan moss extract, and 0.0001-3 parts of guava leaf extract.

[0006] The firming and anti-wrinkle composition provided by the present invention has good compounding effect by selecting components within a specific mass range. It can be applied in cosmetics to effectively tighten the skin, increase skin elasticity, and reduce the occurrence of skin wrinkles. At the same time, it also has excellent moisturizing effect and skin lightening effect.

[0007] Specifically, the present invention has found that platinum powder has a good antioxidant effect, can soothe the skin, reduce discomfort symptoms such as skin redness and swelling, and can also provide luster to the skin and enhance the glossiness of the skin; palmitoyl pentapeptide-4 can stimulate the synthesis of collagen, glycosaminoglycans and hyaluronic acid, stimulate the growth of fibroblasts, effectively reduce fine lines on the skin, improve wrinkles, and enhance the firmness and glossiness of the skin; carnosine is a good stabilizer of cell membranes, has the function of protecting cell membranes, and also has a good antioxidant effect, removes excess free radicals in the skin, reduces skin aging, increases skin elasticity, and can also whiten the skin and improve skin gloss; Balkan lettuce is called resurrection grass, which has a good repair function and has a super strong moisturizing ability. Balkan lettuce extract can rebuild, activate and moisturize the skin, increase skin elasticity and moisture retention, and thus improve skin gloss; guava leaf extract can not only whiten the skin, but also has a good antioxidant effect and can help increase skin elasticity. Furthermore, the present invention has found that the five components selected by the present invention have a good compounding effect. For example, the Balkan chicory extract provided by the present invention can not only increase skin elasticity by itself, but also has an excellent and long-lasting moisturizing effect that can facilitate better absorption of other substances, thereby achieving a better effect. In addition, the platinum powder in the present invention can effectively activate the skin, and cooperate with palmitoyl pentapeptide-4 and carnosine, etc., to effectively enhance the effect of palmitoyl pentapeptide-4 and carnosine on improving skin elasticity, and can also further promote the long-lasting moisturizing effect of the Balkan chicory extract. In addition, the guava leaf extract can also interact with the platinum powder and other components to achieve a more excellent antioxidant effect and brighten the skin tone, thereby reducing the formation of wrinkles from the source and improving skin gloss.

[0008] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, the firming and anti-wrinkle composition comprises the following components in parts by weight: 0.0005-0.002 parts of platinum powder, 1-5 parts of palmitoyl pentapeptide-4, 0.05-0.4 parts of carnosine, 0.1-0.5 parts of Balkan moss extract, and 0.3-1.4 parts of guava leaf extract.

[0009] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, the firming and anti-wrinkle composition comprises the following components in parts by weight: 0.001-0.0015 parts of platinum powder, 2-3 parts of palmitoyl pentapeptide-4, 0.2-0.3 parts of carnosine, 0.2-0.3 parts of Balkan moss extract, and 0.5-0.8 parts of guava leaf extract.

[0010] The present invention has found that the mass parts of the components in the firming and anti-wrinkle composition will affect the comprehensive effect of the firming and anti-wrinkle composition. When the mass parts of the components are further selected to be within the above range, when the obtained firming and anti-wrinkle composition is applied to cosmetics, the obtained cosmetics have better firming effect, moisturizing effect, wrinkle removal effect and skin brightening effect.

[0011] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, in the firming and anti-wrinkle composition, the mass ratio of the sum of the mass of palmitoyl pentapeptide-4 and carnosine to the mass of the Balkan moss extract is (10-14):1.

[0012] Illustratively, in the firming and anti-wrinkle composition, the mass ratio of the sum of the masses of palmitoyl pentapeptide-4 and carnosine to the Balkan moss extract can be any point value or any two point range values ​​between (10-14):1, for example, it can be 10:1, 11:1, 12:1, 13:1, 14:1, etc.

[0013] The present invention has found that the mass ratio of the sum of the masses of palmitoyl pentapeptide-4 and carnosine to the Balkan chicory extract has an impact on the moisturizing effect, increase in skin elasticity and anti-wrinkle effect of the product. When the mass ratio of the sum of the masses of palmitoyl pentapeptide-4 and carnosine to the Balkan chicory extract is further selected to be (10-14):1, the comprehensive effect of the obtained product is better.

[0014] Preferably, in the firming and anti-wrinkle composition, the mass ratio of the sum of the mass of palmitoyl pentapeptide-4 and carnosine to the mass of the Balkan moss extract is (11-12):1.

[0015] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, in the firming and anti-wrinkle composition, the mass percentage of guava leaf extract is 5-50%.

[0016] Exemplarily, in the firming and anti-wrinkle composition, the mass percentage of guava leaf extract can be any point value or any two points in the range of 5-50%, for example, it can be 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, etc.

[0017] The present invention has found that the mass percentage of guava leaf extract in the firming and anti-wrinkle composition will affect the comprehensive performance of the product. When the mass percentage of guava leaf extract is further selected to be within the above range, the obtained product has better anti-wrinkle performance and better moisturizing effect.

[0018] Preferably, in the firming and anti-wrinkle composition, the mass percentage of guava leaf extract is 15-20%.

[0019] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, the average particle size of the platinum powder is 0.15-0.50 μm.

[0020] Preferably, the average particle size of the platinum powder is 0.2-0.3 μm.

[0021] Exemplarily, the average particle size of the platinum powder may be any point value or any two point range values ​​between 0.15-0.40μm, such as 0.15μm, 0.17μm, 0.19μm, 0.2μm, 0.22μm, 0.24μm, 0.26μm, 0.28μm, 0.3μm, 0.32μm, 0.34μm, 0.36μm, 0.38μm, 0.4μm, 0.42μm, 0.44μm, 0.46μm, 0.48μm, 0.5μm, etc.

[0022] It should be noted that the platinum powder within the specific average particle size range described in the present invention can be purchased from conventional markets; or can be prepared by conventional methods; the corresponding platinum powder can be further crushed and sifted to obtain platinum powder within the specific average particle size range.

[0023] The present invention has found that the average particle size of platinum powder not only directly affects the effect of the platinum powder itself on the skin, but also affects the uniform dispersion of the platinum powder and other components, thereby affecting the coordination with other components and further affecting the overall effect of the product; when the average particle size of the platinum powder is further selected to be 0.15-0.50 μm, the overall performance of the obtained product is better; however, considering factors such as preparation and cost, it is further preferred that the average particle size of the platinum powder is 0.2-0.3 μm.

[0024] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, the preparation method of the Balkan lettuce extract includes the following steps: taking Balkan lettuce, drying it, crushing it, then adding it into an ethanol aqueous solution for immersion, ultrasonic extraction after the immersion, filtering after the extraction, collecting the filtrate and concentrating and drying it to obtain the Balkan lettuce extract.

[0025] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, the immersion temperature is 20-40° C., and the immersion time is 12-24 hours.

[0026] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, the power of the ultrasound is 120-160W, the time of the ultrasound is 10-40min, and the temperature of the ultrasound is 40-55°C.

[0027] Preferably, the mass percentage of ethanol in the ethanol aqueous solution is 30-60%.

[0028] Preferably, the mass to volume ratio of the Balkan lettuce and the ethanol aqueous solution is 1 g: (6-12) mL.

[0029] The present invention has found that the selection of the preparation method of the Balkan chicory extract will affect the type and content of the active ingredients therein. When the preparation method of the Balkan chicory extract is further selected as given in the present invention, the obtained Balkan chicory extract can achieve a better compounding effect with other components, thereby achieving a better comprehensive effect of the product.

[0030] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, the preparation method of the guava leaf extract comprises the following steps: taking guava leaves, drying them, crushing them, and then enzymolyzing them. After the enzymolysis is completed, the enzymolysis products are kept warm to obtain enzymolysis products, and the enzymolysis products are added to ethanol aqueous solution for extraction. After the extraction is completed, the filtrate is filtered, and the filtrate is collected and concentrated to dryness.

[0031] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, in the enzymatic hydrolysis, the enzymes include cellulase and pectinase in a mass ratio of 1:(1-4).

[0032] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, in the enzymatic hydrolysis, the added mass of the enzyme is 0.1-0.5% of the mass of the guava leaves.

[0033] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, in the enzymatic hydrolysis, the enzymatic hydrolysis time is 2-4 hours and the enzymatic hydrolysis temperature is 30-45°C.

[0034] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, the mass percentage of ethanol in the ethanol aqueous solution is 40-70%.

[0035] As a preferred embodiment of the firming and anti-wrinkle composition of the present invention, the extraction temperature is 60-90° C., and the extraction time is 2-6 hours.

[0036] Preferably, the mass volume ratio of the enzymatic hydrolysate to the ethanol aqueous solution is 1 g: (8-15) mL.

[0037] Preferably, the insulation temperature is 80-100° C., and the insulation time is 1-3 hours.

[0038] The guava leaf extract provided by the present invention is first subjected to enzymatic hydrolysis and then to heating extraction, so that not only the corresponding active substances can be better extracted, but also the content of the obtained active substances is higher; when the guava leaf extract is applied to a firming and anti-wrinkle composition, the comprehensive performance of the obtained product is better.

[0039] In a second aspect of the present invention, the present invention provides a method for preparing the firming and anti-wrinkle composition, the preparation method comprising the following steps:

[0040] (1) adding platinum powder to an organic solvent and heating under reflux, then cooling to room temperature and standing, then filtering and collecting the residue and drying it to obtain pretreated platinum powder;

[0041] (2) The pretreated platinum powder, palmitoyl pentapeptide-4, carnosine, Balkan chicory extract, and guava leaf extract are mixed evenly to obtain a firming and anti-wrinkle composition.

[0042] In the firming and anti-wrinkle composition provided by the present invention, by pre-treating the platinum powder first and then mixing it with other components, a better mixing and dispersing effect between the components can be achieved.

[0043] As a preferred embodiment of the preparation method of the present invention, in the step (1), the organic solvent includes at least one of methanol, ethanol, and ethanol-water solution.

[0044] As a preferred embodiment of the preparation method of the present invention, in the step (1), the heating reflux time is 1-4h.

[0045] As a preferred embodiment of the preparation method of the present invention, in the step (1), the standing time is 12-24 hours.

[0046] In the third aspect of the present invention, the present invention provides use of the firming and anti-wrinkle composition in the preparation of cosmetics.

[0047] As a preferred embodiment of the application of the present invention, the cosmetics include any one of lotion, emulsion, cream, mask, essence, and spray.

[0048] In a fourth aspect of the present invention, the present invention provides an emulsion, comprising the firming and anti-wrinkle composition of the present invention.

[0049] As a preferred embodiment of the emulsion of the present invention, the emulsion comprises the following components in percentage by mass: 1-8% of the firming and anti-wrinkle composition of the present invention, 2-40% of a cosmetic base, and the balance water.

[0050] As a preferred embodiment of the wrinkle-removing and firming composition of the present invention, the cosmetic matrix includes at least one of a thickener, a moisturizer, an emulsifier, a preservative, a fragrance, and a pH adjuster.

[0051] Exemplarily, the thickener includes at least one of xanthan gum, carbomer, and sorbitan isostearate; the moisturizer includes at least one of D-panthenol, glycerin, butylene glycol, isononyl isononanoate, squalane, and caprylic / capric triglyceride; the emulsifier includes at least one of cetearyl olivate, sorbitan olivate, coconut oil-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, cetearyl glucoside, polyglyceryl-6 distearate, and polymethylsilsesquioxane; the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, and parahydroxyacetophenone; the pH adjuster includes at least one of arginine and disodium EDTA.

[0052] Compared with the prior art, the present invention has the following beneficial effects:

[0053] The firming and anti-wrinkle composition provided by the present invention has good compounding effect between the components by selecting components within a specific mass range, and can be applied to cosmetics to effectively tighten the skin, increase skin elasticity, and reduce the generation of skin wrinkles, and at the same time, it also has excellent moisturizing effect and skin lightening effect. At the same time, the preparation method of the firming and anti-wrinkle composition provided by the present invention is simple to operate, and the raw material source is wide, which is conducive to actual production. DETAILED DESCRIPTION

[0054] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0055] Unless otherwise specified, the reagents, methods and equipment used in the present invention are all conventional reagents, methods and equipment in the art; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0056] Platinum powder 1: average particle size 0.3 μm, commercially available;

[0057] Platinum powder 2: average particle size 0.2 μm, commercially available;

[0058] Platinum powder 3: average particle size 0.5 μm, commercially available;

[0059] Balkan lettuce extract 1: homemade, the preparation method includes the following steps: take Balkan lettuce leaves, dry them, crush them, and pass them through an 80-mesh sieve, collect the sieve under the sieve to obtain the crushed Balkan lettuce, then add 30% ethanol aqueous solution (the mass volume ratio of the crushed Balkan lettuce to the ethanol aqueous solution is 1g:10mL), then immerse at 35°C for 12h, ultrasonicate at 120W and 55°C for 15min after the immersion, filter after the ultrasonicate, collect the filter residue and concentrate it, and then place it at 40°C for 24h to obtain Balkan lettuce extract 1;

[0060] Balkan lettuce extract 2: homemade, the preparation method includes the following steps: take Balkan lettuce leaves, dry them, crush them, and pass them through an 80-mesh sieve, collect the sieve under the sieve to obtain the crushed Balkan lettuce, then add 60% ethanol aqueous solution (the mass volume ratio of the crushed Balkan lettuce to the ethanol aqueous solution is 1g:6mL), then immerse at 25°C for 24h, ultrasonicate at 160W and 40°C for 35min after the immersion, filter after the ultrasonicate, collect the filter residue and concentrate it, and then place it at 40°C for 24h to obtain Balkan lettuce extract 2;

[0061] Balkan lettuce extract 3: homemade, the preparation method includes the following steps: take Balkan lettuce leaves, dry them, crush them, and pass them through an 80-mesh sieve, collect the sieve under the sieve to obtain the crushed Balkan lettuce, then add 30% ethanol aqueous solution (the mass volume ratio of the crushed Balkan lettuce to the ethanol aqueous solution is 1g:10mL), then immerse at 35°C for 14.25h, filter after immersion, collect the filter residue and concentrate it, and then place it at 40°C for 24h in vacuum drying to obtain Balkan lettuce extract 3;

[0062] Balkan lettuce extract 4: homemade, the preparation method includes the following steps: taking Balkan lettuce leaves, drying them, crushing them, and passing them through an 80-mesh sieve, collecting the sieve under the sieve to obtain the crushed Balkan lettuce, then adding 30% ethanol aqueous solution (the mass volume ratio of the crushed Balkan lettuce to the ethanol aqueous solution is 1g:10mL), then ultrasonicating at 120W and 55°C for 14.25h, filtering after the ultrasonication, collecting the filter residue and concentrating it, and then placing it at 40°C for 24h in vacuum drying to obtain Balkan lettuce extract 4;

[0063] Guava leaf extract 1: homemade, the preparation method includes the following steps: take guava leaves, dry them, crush them and pass them through a 120-mesh sieve, collect the sieve under the sieve to obtain the crushed guava leaves, then add water to the guava leaves and grind them into slurry (the mass ratio of water to the crushed guava leaves is 1:1), then add cellulase and pectinase in a mass ratio of 1:1 (the total mass of cellulase and pectinase is 0.1% of the mass of the crushed guava leaves), and enzymolyze at 40°C for 4h, heat to 90°C after enzymolysis and keep for 1h to obtain enzymolysis products, then add the enzymolysis products to 60% ethanol aqueous solution (the mass volume ratio of enzymolysis products to ethanol aqueous solution is 1g:10mL), and extract at 70°C for 6h, filter after the extraction, collect the filter residue and concentrate it, and then place it at 40°C for vacuum drying for 24h to obtain guava leaf extract 1;

[0064] Guava leaf extract 2: homemade, the preparation method includes the following steps: take guava leaves, dry them, crush them and pass them through a 120-mesh sieve, collect the sieve under the sieve to obtain the crushed guava leaves, then add water to the guava leaves and grind them into a slurry (the mass ratio of water to the crushed guava roots is 1:1), then add cellulase and pectinase in a mass ratio of 1:4 (the total mass of cellulase and pectinase is 0.5% of the mass of the crushed guava roots), and enzymolyze at 30°C for 2h, heat to 90°C after enzymolysis and keep for 1h to obtain an enzymolysis product, then add the enzymolysis product to 40% ethanol aqueous solution (the mass volume ratio of the enzymolysis product and the ethanol aqueous solution is 1g:10mL), and extract at 90°C for 2h, filter after the extraction, collect the filter residue and concentrate it, and then place it at 40°C for vacuum drying for 24h to obtain guava leaf extract 2;

[0065] Guava leaf extract 3: homemade, the preparation method includes the following steps: take guava leaves, dry them, crush them and pass them through a 120-mesh sieve, collect the sieve under the sieve to obtain the crushed guava leaves, then add water to the guava leaves and grind them into a slurry (the mass ratio of water to the crushed guava roots is 1:1), then add cellulase (the mass of the cellulase is 0.1% of the mass of the crushed guava roots), and enzymolyze at 30°C for 2h, heat to 90°C for 1h after enzymolysis to obtain an enzymolysis product, then add the enzymolysis product to 60% ethanol aqueous solution (the mass volume ratio of the enzymolysis product to the ethanol aqueous solution is 1g:10mL), and extract at 90°C for 2h, filter after the extraction, collect the filter residue and concentrate it, and then place it at 40°C for vacuum drying for 24h to obtain guava leaf extract 3;

[0066] Guava leaf extract 4: homemade, the preparation method includes the following steps: take guava leaves, dry them, crush them and pass them through a 120-mesh sieve, collect the sieve underflow to obtain the crushed guava leaves, then add 60% ethanol aqueous solution to the guava leaves (the mass volume ratio of the crushed guava leaves and the ethanol aqueous solution is 1g:10mL), and extract at 90°C for 4h, filter after the extraction, collect the filter residue and concentrate it, then place it at 40°C and vacuum dry it for 24h to obtain guava leaf extract 4.

[0067] Examples 1-8 and Comparative Examples 1-4

[0068] The examples and comparative examples of the present invention provide a firming and anti-wrinkle composition, the components (parts by mass) of the firming and anti-wrinkle composition are shown in Table 1-2;

[0069] Table 1

[0070]

[0071] The preparation method of the firming and anti-wrinkle composition provided in Example 1 comprises the following steps:

[0072] (1) Platinum powder was added to methanol and heated under reflux for 2 h, then cooled to room temperature and allowed to stand for 20 h, and then filtered to collect the residue and dried to obtain pretreated platinum powder;

[0073] (2) mixing the pretreated platinum powder, palmitoyl pentapeptide-4, carnosine, Balkan chicory extract, and guava leaf extract to obtain a firming and anti-wrinkle composition;

[0074] The preparation methods of Examples 2-8 and Comparative Examples 1-4 are consistent with that of Example 1, except that no relevant components are added.

[0075] Example 9

[0076] The embodiment of the present invention provides a firming and anti-wrinkle composition. The only difference between the firming and anti-wrinkle composition and the embodiment 1 is that platinum powder 2 is used instead of platinum powder 1.

[0077] Example 10

[0078] The embodiment of the present invention provides a firming and anti-wrinkle composition, the only difference between the firming and anti-wrinkle composition and the embodiment 1 is that platinum powder 3 is used instead of platinum powder 1.

[0079] Embodiment 11

[0080] The embodiment of the present invention provides a firming and anti-wrinkle composition. The only difference between the firming and anti-wrinkle composition and the embodiment 1 is that the Balkan spathiphyllum extract 2 is used to replace the Balkan spathiphyllum extract 1.

[0081] Example 12

[0082] The embodiment of the present invention provides a firming and anti-wrinkle composition. The only difference between the firming and anti-wrinkle composition and the embodiment 1 is that the Balkan spathiphyllum extract 3 is used to replace the Balkan spathiphyllum extract 1.

[0083] Example 13

[0084] The embodiment of the present invention provides a firming and anti-wrinkle composition. The only difference between the firming and anti-wrinkle composition and the embodiment 1 is that the Balkan spathiphyllum extract 4 is used to replace the Balkan spathiphyllum extract 1.

[0085] Embodiment 14

[0086] The embodiment of the present invention provides a firming and anti-wrinkle composition, wherein the only difference between the firming and anti-wrinkle composition and the embodiment 1 is that the guava leaf extract 2 is used instead of the guava root extract 1.

[0087] Embodiment 15

[0088] The embodiment of the present invention provides a firming and anti-wrinkle composition. The only difference between the firming and anti-wrinkle composition and the embodiment 1 is that the guava leaf extract 3 is used instead of the guava root extract 1.

[0089] Example 16

[0090] The present invention provides a firming and anti-wrinkle composition according to the embodiment of the present invention. The only difference between the firming and anti-wrinkle composition and the embodiment of the present invention is that the guava leaf extract 4 is used to replace the guava root extract 1.

[0091] Comparative Example 5

[0092] The comparative example of the present invention provides a firming and anti-wrinkle composition, the only difference between the firming and anti-wrinkle composition and Example 1 is that the Balkan moss extract 1 is replaced with the aloe vera leaf extract.

[0093] Comparative Example 6

[0094] The comparative example of the present invention provides a firming and anti-wrinkle composition, the only difference between the firming and anti-wrinkle composition and Example 1 is that guava fruit extract is used instead of guava leaf extract 1.

[0095] Comparative Example 7

[0096] The comparative example of the present invention provides a firming and anti-wrinkle composition, the only difference between the firming and anti-wrinkle composition and Example 1 is that tripeptide is used instead of carnosine.

[0097] Comparative Example 8

[0098] The comparative example of the present invention provides a firming and anti-wrinkle composition, the only difference between the firming and anti-wrinkle composition and Example 1 is that acetyl hexapeptide-8 is used instead of palmitoyl pentapeptide-4.

[0099] Application Example 1-17 and Comparative Application Example 1-9

[0100] The application examples of the present invention and the comparative application examples provide an emulsion, the components (mass percentage) of the emulsion are shown in Table 3; wherein the firming and anti-wrinkle compositions in application examples 1-16 are the firming and anti-wrinkle compositions prepared in embodiments 1-16, respectively, the firming and anti-wrinkle compositions in comparative application examples 1-8 are the firming and anti-wrinkle compositions in comparative examples 1-8, respectively, and the firming and anti-wrinkle composition used in application example 17 is the firming and anti-wrinkle composition prepared in embodiment 1;

[0101] Table 3

[0102]

[0103]

[0104] The preparation method of the emulsion provided in Application Example 1 comprises the following steps:

[0105] (1) Mixing the humectant and the thickener with deionized water, heating to 85° C., homogenizing at a speed of 1000 rpm for 5 minutes, and keeping the mixture warm for later use to obtain a prefabricated component A;

[0106] (2) mixing the emulsifier, heating to 85° C., homogenizing at a speed of 1000 rpm for 5 minutes, and keeping warm for later use after the homogenization to obtain a prefabricated component B;

[0107] (3) mixing the preservatives and heating to 60° C. to melt to obtain a prefabricated C component;

[0108] (4) The preformed component A is heated to 80°C, and the preformed component B is added at a speed of 400 rpm, and stirred and mixed. Then, the temperature is lowered to 60°C, and the preformed component C is added at a speed of 400 rpm and stirred and mixed. Then, the temperature is lowered to 40°C, and the firming and anti-wrinkle composition is added and stirred for 6 minutes. Finally, the remaining pH adjuster is added to adjust the pH to 6.0, and the stirring is stopped. The material is discharged to obtain an emulsion.

[0109] The preparation methods of the emulsions in Application Examples 2-17 and Comparative Application Examples 1-9 are consistent with that in Application Example 1, except that no related components are added.

[0110] Effect Example 1

[0111] The effect examples of the present invention verify the promoting effect and anti-oxidation effect of the firming and anti-wrinkle compositions prepared in Examples 1-16 and Comparative Examples 1-8 on elastin;

[0112] 1. Promoting effect on elastin

[0113] The test was conducted in accordance with the standard T / ZHCA 015-2022 "Evaluation of the Firming Efficacy of Cosmetics by Relative Expression of Elastin Gene in Zebrafish Fry", and three concentration groups were set during the test;

[0114] The gene expression promotion rate was calculated based on the gene expression level obtained in the test, and the gene expression promotion rate = (gene expression level in the blank group - gene expression level in the test group) / gene expression level in the blank group × 100%. The calculated results of the Eln1 gene expression promotion rate are shown in Table 4;

[0115] 2. Antioxidant Effect

[0116] The test was carried out according to T / SHRH 006-2018 Cosmetics - Free Radical (DPPH) Scavenging Experimental Method, and the 1h scavenging rate was recorded in Table 4;

[0117] Table 4

[0118]

[0119]

[0120] As can be seen from Table 4, when the technical solution provided by the present invention is adopted, the obtained firming and anti-wrinkle composition has excellent effects on promoting the expression of elastin gene and scavenging DPPH; specifically, the obtained firming and anti-wrinkle composition has an expression promotion rate of elastin gene Eln1 of more than 71%, and a 1h scavenging rate of DPPH of more than 82%;

[0121] It can be seen from Examples 1-8 that the mass fraction of the components in the firming and anti-wrinkle composition affects the performance of the product. When the mass fraction of the components in the firming and anti-wrinkle composition is further selected to be within the preferred range given in the present invention, the comprehensive performance of the obtained product is better. Specifically, the obtained firming and anti-wrinkle composition has an expression promotion rate of elastin gene Eln1 of more than 80%, and a 1h scavenging rate of DPPH of more than 88%;

[0122] It can be seen from Example 1 and Comparative Examples 1-4 that when one of the components of the present invention is not added, the obtained firming and anti-wrinkle composition has a significant downward trend in the promotion rate of elastin genes and the scavenging rate of DPPH; it can be seen from Example 1 and Comparative Examples 5-8 that when other similar ingredients are used to replace the components of the present invention, the effects of the present invention cannot be achieved.

[0123] Effect Example 2

[0124] The effect examples of the present invention verify the safety of the emulsions provided by application examples 1-17 and comparative application examples 1-9, and specifically include the following steps:

[0125] 30 volunteers aged between 20 and 50 were selected, the wells of the spot tester were numbered, and the test samples were placed in the wells of the spot tester with the corresponding numbers, with a dosage of 0.02 mL per well; the spot tester with the test substance was applied to the back of the subject with a low-allergenic tape, and the palm of the hand was gently pressed to make it evenly applied to the skin for 24 hours. After that, the spot tester was removed and the skin condition of the wound site was observed. The identification standards are shown in Table 5;

[0126] Table 5

[0127] grade symbol Identification criteria 0 － Negative reaction: no irritation, no erythema 1 ± Suspected reaction: Mild erythema 2 + Weak positive reaction: erythema 3 ++ Strong positive reaction: erythema, papules, blisters 4 +++ Very strong positive reaction: severe edema, large blisters

[0128] It has been found through identification that the emulsion prepared by the technical solution of the present invention has low irritation to the skin and meets the requirement of the "Cosmetic Hygiene Standards" for non-irritating cosmetics.

[0129] Effect Example 3

[0130] The effect examples of the present invention verify the human application effects of the emulsions provided by application examples 1-17 and comparative application examples 1-9, specifically comprising the following steps:

[0131] 156 volunteers with obvious facial wrinkles, aged between 30 and 60 years old, were selected and randomly divided into 26 groups, with 6 people in each group; under normal conditions, the volunteers continuously used the corresponding application examples 1-17 and comparative application examples 1-9 on their entire faces for 28 days, and the efficacy of the application examples and comparative application examples was evaluated;

[0132] Specifically: every morning and evening, after cleansing and moisturizing, volunteers should apply an appropriate amount of lotion to the face and massage until absorbed. Use it on the entire face once in the morning and evening, and return visits will be made on days 0, 7, 14, and 28. On the day of the volunteer's visit, the test area should wash their face with clean water without applying any products. They should sit quietly in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes. Instruments were used to quantify and photograph the improvement of facial indicators, including the use of Primos to measure the area of ​​nasolabial folds; the use of a transepidermal water loss probe to measure the skin TEWL value was tested by TM Hex (Courage+Khazaka), ITA° value was tested by Colorimeter CL400, skin firmness parameter F4 was tested by Cutometer (MPA580, Courage and Khazaka, Germany), and then the improvement rate at 28 days was calculated according to the following formula;

[0133] Improvement rate before and after use = | (X 使用前均值 -X 使用后均值 )∣ / X 使用前均值 ×100%; the results are shown in Table 6;

[0134] Table 6

[0135]

[0136]

[0137] It can be seen from Table 6 that when the technical solution provided by the present invention is adopted, compared with comparative application example 9 (equivalent to the blank group), the obtained emulsion has excellent firming and anti-wrinkle, skin brightening and moisturizing effects; specifically, after being applied to the human body for 28 days, the improvement rate (specifically the reduction rate) of the nasolabial fold area is more than 21.14%, the improvement rate of the TEWL value is more than 12.66%, the improvement rate of the ITA° value is more than 10.01%, and the improvement rate of the F4 value is more than 11.23%;

[0138] It can be seen from Application Examples 1-8 that the mass fraction of the components in the firming and anti-wrinkle composition will affect the comprehensive performance of the product. When the mass fraction of the components in the firming and anti-wrinkle composition is further selected to be within the preferred range of the present invention, the comprehensive performance of the obtained product is better. Specifically, after being applied to the human body for 28 days, the improvement rate (specifically, the reduction rate) of the nasolabial fold area is above 26.05%, the improvement rate of the TEWL value is above 16.57%, the improvement rate of the ITA° value is above 14.24%, and the improvement rate of the F4 value is above 15.15%. It can be seen from Application Examples 1 and Application Examples 9-16 that the parameters of the components in the firming and anti-wrinkle composition or the preparation method of the extract will also affect the comprehensive performance of the product to a certain extent.

[0139] It can be seen from Application Example 1 and Comparative Application Examples 1-4 that when a certain component is missing in the added firming and anti-wrinkle composition, the comprehensive effect of the obtained emulsion is significantly reduced; it can be seen from Application Example 1 and Comparative Application Examples 5-8 that when other similar components are used to replace the components in the firming and anti-wrinkle composition of the present invention, the obtained emulsion cannot achieve the effect of the present invention.

[0140] Finally, it should be noted that the above embodiments are intended to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A firming and anti-wrinkle composition, characterized in that: The firming and anti-wrinkle composition comprises the following components in parts by weight: 0.0001-0.005 parts of platinum powder, 0.0001-18 parts of palmitoyl pentapeptide-4, 0.0001-10 parts of carnosine, 0.0001-1 parts of Balkan moss extract, and 0.0001-3 parts of guava leaf extract.

2. The firming and anti-wrinkle composition according to claim 1, characterized in that: The firming and anti-wrinkle composition comprises the following components in parts by weight: 0.0005-0.002 parts of platinum powder, 1-5 parts of palmitoyl pentapeptide-4, 0.05-0.4 parts of carnosine, 0.1-0.5 parts of Balkan moss extract, and 0.3-1.4 parts of guava leaf extract.

3. The firming and anti-wrinkle composition according to claim 1, characterized in that: The firming and anti-wrinkle composition comprises the following components in parts by weight: 0.001-0.0015 parts of platinum powder, 2-3 parts of palmitoyl pentapeptide-4, 0.2-0.3 parts of carnosine, 0.2-0.3 parts of Balkan moss extract, and 0.5-0.8 parts of guava leaf extract.

4. The firming and anti-wrinkle composition according to claim 1, characterized in that: In the firming and anti-wrinkle composition, the mass ratio of the sum of the mass of palmitoyl pentapeptide-4 and carnosine to the mass of the Balkan moss extract is (10-14):

1.

5. The firming and anti-wrinkle composition according to claim 1, characterized in that: In the firming and anti-wrinkle composition, the mass percentage of guava leaf extract is 5-50%.

6. The firming and anti-wrinkle composition according to claim 1, characterized in that: The average particle size of the platinum powder is 0.15-0.50 μm.

7. The firming and anti-wrinkle composition according to claim 6, characterized in that: The preparation method of the Balkan chicory extract comprises the following steps: taking Balkan chicory, drying it, crushing it, then adding it into an ethanol aqueous solution for immersion, ultrasonic extraction after the immersion, filtering after the extraction, collecting the filtrate and concentrating and drying it to obtain the Balkan chicory extract.

8. The firming and anti-wrinkle composition according to claim 7, characterized in that: The immersion temperature is 20-40°C and the immersion time is 12-24h; And / or, the ultrasonic power is 120-160W, the ultrasonic time is 10-40min, and the ultrasonic temperature is 40-55°C.

9. The firming and anti-wrinkle composition according to claim 1, characterized in that: The preparation method of the guava leaf extract comprises the following steps: taking guava leaves, drying them, crushing them, and then enzymolyzing them; keeping them warm after the enzymolysis is completed to obtain enzymolysis products; adding the enzymolysis products to an ethanol aqueous solution for extraction; filtering after the extraction is completed; collecting the filtrate and concentrating and drying it.

10. The firming and anti-wrinkle composition according to claim 9, characterized in that: In the enzymolysis, the enzymes include cellulase and pectinase in a mass ratio of 1:(1-4); And / or, in the enzymolysis, the added mass of the enzyme is 0.1-0.5% of the mass of the guava fruit; And / or, in the enzymolysis, the enzymolysis time is 2-4 hours and the enzymolysis temperature is 30-45°C.

11. The firming and anti-wrinkle composition according to claim 9, characterized in that: The mass percentage of ethanol in the ethanol aqueous solution is 40-70%; And / or, the extraction temperature is 60-90°C, and the extraction time is 2-6h.

12. The method for preparing the firming and anti-wrinkle composition according to any one of claims 1 to 11, characterized in that: The preparation method comprises the following steps: (1) adding platinum powder to an organic solvent and heating under reflux, then cooling to room temperature and standing, then filtering and collecting the residue and drying it to obtain pretreated platinum powder; (2) The pretreated platinum powder, palmitoyl pentapeptide-4, carnosine, Balkan chicory extract, and guava leaf extract are mixed evenly to obtain a firming and anti-wrinkle composition.

13. Use of the firming and anti-wrinkle composition according to any one of claims 1 to 11 in the preparation of cosmetics.

14. An emulsion, characterized in that The emulsion comprises the firming and anti-wrinkle composition according to any one of claims 1 to 11.

15. The emulsion according to claim 14, characterized in that The emulsion comprises the following components in percentage by weight: 1-8% of the firming and anti-wrinkle composition according to any one of claims 1 to 11, 2-40% of a cosmetic base, and the balance water.

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