Soothing and moisturizing composition, preparation method, application and skin care product

Abstract

The invention belongs to the technical field of skin care, and discloses a soothing and moisturizing composition, a preparation method, application and a skincare product, the soothing and moisturizing composition is obtained by taking jujube fruits, Chinese wolfberry fruits and centella as fermentation substrates and fermenting saccharomycetes and / or galactose yeast-like bacteria in the fermentation substrates, the weight ratio of the jujube fruits to the Chinese wolfberry fruits to the centella asiatica is (1-5): (2-8): (1-2). The soothing and moisturizing composition obtained by combined fermentation of the jujube fruits, the Chinese wolfberry fruits and the centella asiatica as fermentation substrates can generate a synergistic effect, so that the skin soothing, moisturizing and antioxidant effects of the soothing and moisturizing composition are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care, and in particular to a soothing and moisturizing composition, a preparation method, a use and a skin care product. Background Art

[0002] The definition of soothing in the context of skin care products mainly refers to the use of specific ingredients and mechanisms of action to reduce or eliminate discomfort, irritation, sensitivity and other conditions caused by various internal and external factors, so that the skin can return to a relatively calm, comfortable and stable state.

[0003] It emphasizes a relieving, harmonizing, and soothing effect. Specifically, it relieves skin discomfort such as redness, fever, itching, and tingling, reduces the skin's overreaction to stimuli, helps sensitive skin reduce the frequency and severity of allergic symptoms, and allows stressed skin to return to normal as soon as possible, while improving the skin's ability and tolerance to external adverse factors. Soothing is not only about improving current symptoms, but also about maintaining the health and stability of the skin in the long term.

[0004] Skin soothing is mainly achieved through external means. It does not directly repair skin tissue, but maintains skin health and comfort by alleviating skin discomfort. This kind of soothing can be understood as "decompression" of the skin, especially when the skin is out of balance due to long-term stress or other external factors, soothing conditioning can help the skin return to a stable state.

[0005] Currently, mainstream soothing skin care products use plants as raw materials, such as prior art 1: Chinese patent application 202410961920.3 discloses a composition with soothing, whitening and firming effects and its application, the composition comprising: ascorbic acid, Mirabilis jalapa extract, carnosine, kapok flower extract, ubiquinone, β-glucan, panthenol, bisabolol and ginger root extract.

[0006] Existing technology 1 uses multiple active ingredients to cooperate with each other and enhance synergy, which can effectively moisturize, repair the skin barrier, comprehensively slow down the oxidation of cells, reduce the production of free radicals, brighten the skin tone, reduce melanin synthesis, promote the metabolism of skin cells, prevent skin aging and sagging, and reduce wrinkles, and has excellent soothing, whitening and firming effects.

[0007] Similarly, prior art 2: Chinese patent application 202410826701.4 discloses an olive soothing complex with soothing synergistic effects, and its preparation method and application. The olive soothing complex includes an olive leaf extract with soothing efficacy and a citrus fruit extract that has a soothing and synergistic effect on the olive leaf extract, specifically including: 5 to 10 parts of olive leaf extract powder, 45 to 55 parts of citrus fruit extract, 25 to 45 parts of polyol, and 0 to 20 parts of water.

[0008] Existing technology 2 can regulate the capsaicin receptors that mediate discomfort such as burning and stinging on sensitive skin through the olive soothing complex to effectively relieve the discomfort caused to the skin by external stimuli, and can inhibit the overexpression of keratinocyte TRPV1; it can inhibit the activation of keratinocyte TRPV1 and has a good soothing effect.

[0009] Both prior art 1 and prior art 2 have good skin soothing effects. Both prior art 1 and prior art 2 use plant extracts to form soothing compositions, which is consistent with most of the soothing skin care products on the market. However, the soothing active ingredients of plants can be obtained not only by extraction, but also by biological fermentation process. The biological fermentation technology has three major advantages in cosmetic applications: (1) The product after fermentation has a smaller molecular weight, fits better with the skin, and is easier to absorb, thus further improving the efficacy of the product; (2) Fermentation product extracts have a mysterious effect on regulating skin flora and can regulate the physiological activity of the skin; (3) Potentially harmful substances can be decomposed through the transformation and decomposition of microorganisms to reduce or eliminate potential safety risks.

[0010] Currently, there are few skin care products on the market that use raw materials obtained through bio-fermentation technology as effective ingredients. Therefore, it is necessary to develop a soothing composition that uses raw materials obtained through bio-fermentation technology as a soothing effective ingredient to meet the diverse needs of the market. Summary of the invention

[0011] One of the purposes of the present invention is to provide a soothing and moisturizing composition, which is obtained by using jujube fruit, wolfberry fruit and Centella asiatica as fermentation substrates, and fermenting yeast and / or galactosaccharomyces-like bacteria in the fermentation substrate. The soothing and moisturizing composition is safe and has excellent skin soothing effect, and through the synergistic combination of the three, it also has excellent moisturizing effect.

[0012] Another object of the present invention is to provide a method for preparing a soothing and moisturizing composition, which uses jujube fruit, wolfberry fruit and Centella asiatica as fermentation substrates and ferments them through specific strains and fermentation processes, thereby maximizing the synergistic soothing effects of jujube fruit, wolfberry fruit and Centella asiatica.

[0013] At the same time, the present invention also provides a soothing and moisturizing composition, which is used for preparing cosmetics to improve the excellent skin soothing effect of the cosmetics.

[0014] Furthermore, the present invention provides a skin care product containing the soothing and moisturizing composition of the present invention, which has excellent skin soothing effect.

[0015] To achieve the above-mentioned purpose, the present invention provides a soothing and moisturizing composition, which is obtained by fermenting jujube fruit, wolfberry fruit and Centella asiatica as fermentation substrates, and yeast and / or galactosaccharomyces-like bacteria in the fermentation substrate, and the weight ratio of the jujube fruit, wolfberry fruit and Centella asiatica is 1-5:2-8:1-2.

[0016] In the technical solution of the present invention, jujube fruit, i.e. red jujube, is rich in antioxidant ingredients such as vitamin C, flavonoids and polyphenols, which can remove free radicals in the body and reduce the damage of oxidative stress to the skin, thereby alleviating inflammation and irritation of the skin; and during the fermentation process of jujube fruit, polysaccharides and amino acids are released, and these ingredients can form a moisturizing film on the skin surface to lock in moisture and prevent moisture evaporation.

[0017] The β-carotene and polyphenols in wolfberry have significant anti-inflammatory effects, which can inhibit the release of inflammatory factors and reduce skin redness and irritation; the active ingredients in wolfberry can promote the regeneration and repair of skin cells, help improve the overall condition of the skin, and enhance skin comfort; the bioactive substances in wolfberry extract can enhance the skin's ability to absorb water and help the skin stay hydrated.

[0018] Centella asiatica and other ingredients in Centella asiatica have strong anti-inflammatory and sedative effects, which can quickly relieve skin discomfort such as redness, swelling, and stinging; Centella asiatica extract can stimulate the regeneration of skin cells, help repair damaged skin barriers, and thus improve the skin's moisturizing ability; the active ingredients in Centella asiatica can form a thin film on the skin surface to lock in moisture, prevent moisture loss, and keep the skin hydrated.

[0019] Preferably, the yeast is one of Saccharomyces cerevisiae, Pichia pastoris, and Kluyveromyces.

[0020] The present invention provides a method for preparing a soothing and moisturizing composition, comprising the following steps: Step 1: performing primary culture on yeast and / or galactosaccharomyces-like bacteria to obtain a fermentation strain activation liquid; Step 2: inoculating the fermentation strain activation liquid for secondary culture to obtain the fermentation strain seed liquid; Step 3: using jujube fruit, wolfberry fruit and Centella asiatica as fermentation substrates to prepare a fermentation medium, then inoculating the fermentation bacteria seed liquid for fermentation, and separating to obtain the soothing and moisturizing composition after the fermentation is completed.

[0021] Preferably, the specific operation of step 3 is: prepare a fermentation medium by mixing 20g of glucose, 5-25g of jujube fruit powder, 10-40g of wolfberry fruit powder, 5-10g of Centella asiatica powder per kg, with the remainder being water, then inoculate the fermentation bacteria seed liquid for fermentation, and separate to obtain the soothing and moisturizing composition after the fermentation is completed.

[0022] Further preferably, the specific operation of inoculating the fermentation bacteria seed liquid for fermentation is: fermentation at a constant temperature of 30°C, and when fermenting for 0-6h, set the speed to 50 rpm; when fermenting for 6-9h, set the speed to 100 rpm; when fermenting for 9-16h, set the dissolved oxygen content value of the fermentation tank to 20%, and the stirring speed and ventilation volume are linked to the dissolved oxygen content, and the speed and ventilation volume are automatically compensated and adjusted; when fermenting for 16-48h, set the speed to 80 rpm; when the fermentation is completed at 48h, stop stirring.

[0023] Furthermore, the separation operation of step 3 is: using a centrifuge at a speed of 15000 rpm to separate the bacteria and the fermentation culture medium, collecting the fermentation supernatant after centrifugation, and then sterilizing the fermentation supernatant at 105°C for 30 minutes at high temperature and high pressure, and after sterilization and cooling to room temperature, clarifying and refining to obtain a soothing and moisturizing composition.

[0024] It should be noted that centrifugal separation and sterilization are conventional operations of biological fermentation and do not affect the soothing effect of the soothing and moisturizing composition of the present invention.

[0025] Step 1 and step 2 of the present invention can adopt existing fermentation culture technology to obtain fermentation strain activation liquid and fermentation strain seed liquid respectively; more specifically, step 1 and step 2 of the present invention can be specifically as follows: Step 1: Prepare activation medium and seed medium by mixing 10g glucose, 5g malt extract and the rest water per kg, adjust the pH value of the solution to 6.0, sterilize the medium at 121℃ for 20min, add 0.5g frozen fermentation liquid (concentration: 1x10 6 CFU / g) were cultured in the activation medium for the first stage; the constant temperature was set at 28°C and the fermentation strain activation liquid was obtained after 24 hours of culture. The concentration of live bacteria in the fermentation strain activation liquid was 1.2x10 8 CFU / g.

[0026] Step 2: After the first-level culture, fresh fermentation strain activation liquid was inoculated into the seed culture medium and cultured at 28°C for 24 hours to obtain the fermentation strain seed liquid. The concentration of live bacteria in the fermentation strain seed liquid was tested to be 2.0x10 9 CFU / g; the weight of the fermentation bacteria activation liquid added to every 1kg of seed culture medium is 1g.

[0027] The present invention also provides a use of the soothing and moisturizing composition for preparing cosmetics.

[0028] The present invention further provides a skin care product comprising 0.01-30 wt % of the soothing and moisturizing composition as described above.

[0029] Preferably, the skin care product is one of an aqueous solution, an essence, an emulsion, and a cream.

[0030] The present invention also discloses a beauty liquid, comprising 83.2-89.1 wt % of the soothing and moisturizing composition, and further comprising 10-15 wt % of glycerol, 0.5-1 wt % of 1,2-hexanediol and 0.4-0.8 wt % of p-hydroxyacetophenone.

[0031] Beneficial Effects

[0032] Compared with the prior art, the present invention has at least the following advantages: (1) The present invention finds that the soothing and moisturizing composition obtained by fermenting jujube fruit, wolfberry fruit and Centella asiatica as fermentation substrates can produce a synergistic effect, so that the skin soothing, moisturizing and antioxidant effects of the soothing and moisturizing composition are improved; (2) The present invention discloses a soothing and moisturizing composition, which is obtained by fermenting jujube fruit, wolfberry fruit and Centella asiatica by biofermentation technology, and has excellent skin adhesion, soothing, moisturizing and antioxidant effects, and is highly safe; (3) The present invention discloses a method for preparing a soothing and moisturizing composition. By using a specific fermentation-like bacteria in combination with a specific fermentation process, the synergistic fermentation effect of jujube fruit, wolfberry fruit and Centella asiatica is fully exerted, thereby maximizing the skin soothing effect of the composition. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] The present invention is further described below in conjunction with the accompanying drawings and embodiments; Figure 1 This is a chicken embryo observation picture before using the soothing and moisturizing composition of Example 6 of the present invention; Figure 2 This is a picture of chicken embryo observation after using the soothing and moisturizing composition of Example 6 of the present invention. DETAILED DESCRIPTION

[0034] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0035] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0036] In the following examples and comparative examples, the brewer's yeast was deposited in Guangdong Microbiological Culture Collection Center (GDMCC), classified and named Saccharomyces sp., Latin name Saccharomyces sp., preservation number GDMCC NO: 65073, preservation date: August 29, 2024, preservation address: 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, concentration: 1x10 6 CFU / g; the galactose yeast-like bacteria are preserved in the Guangdong Microbial Culture Collection Center (GDMCC), and are classified and named Geotrichum candidum, and the Latin name is Geotrichum candidum , Deposit number: GDMCCNO: 64235, Deposit date: January 2, 2024, Deposit address: 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou, Concentration: 1x10 6 CFU / g.

[0037] It should be noted that the concentration of the above fermentation bacteria can be adjusted, and the technical solution of the present invention can be fermented by yeast and / or galactosyl yeast-like bacteria of other concentrations.

[0038] In the following examples and comparative examples, the fermentation bacteria seed liquid was prepared by the following method: Step 1: Prepare activation medium and seed medium by mixing 10g glucose, 5g malt extract and the rest water per kg, adjust the pH value of the solution to 6.0, sterilize the medium at 121℃ for 20min, add 0.5g frozen fermentation liquid (concentration: 1x10 6 CFU / g) were cultured in the activation medium for the first stage; the constant temperature was set at 28°C and the fermentation strain activation liquid was obtained after 24 hours of culture. The concentration of live bacteria in the fermentation strain activation liquid was 1.2x10 8 CFU / g.

[0039] Step 2: After the first-level culture, fresh fermentation strain activation liquid was inoculated into the seed culture medium and cultured at 28°C for 24 hours to obtain the fermentation strain seed liquid. The concentration of live bacteria in the fermentation strain seed liquid was tested to be 2.0x10 9CFU / g; the weight of the fermentation bacteria activation liquid added to every 1kg of seed culture medium is 1g.

[0040] Example 1 A soothing and moisturizing composition is prepared by the following steps: Step a: preparing a fermentation medium in a ratio of 20 g glucose, 5 g jujube powder, 15 g wolfberry powder, 8 g Centella asiatica powder, and the balance water per kg, then inoculating the fermentation culture medium with a saccharomyces cerevisiae fermentation strain seed solution, and fermenting at a constant temperature of 30° C. When fermenting for 0-6 h, the rotation speed is set to 50 rpm; when fermenting for 6-9 h, the rotation speed is set to 100 rpm; when fermenting for 9-16 h, the dissolved oxygen content value of the fermentation tank is set to 20%, the stirring speed and the ventilation volume are linked to the dissolved oxygen content, and the speed and ventilation volume are automatically compensated and adjusted; when fermenting for 16-48 h, the rotation speed is set to 80 rpm; when fermentation is completed at 48 h, stirring is stopped; Step b: Use a centrifuge at a speed of 15,000 rpm to separate the bacteria and the fermentation culture medium, collect the fermentation supernatant after centrifugation, and then sterilize the fermentation supernatant at 105°C for 30 minutes. After sterilization and cooling to room temperature, clarify and purify to obtain a soothing and moisturizing composition.

[0041] Example 2 The method is generally the same as Example 1, except that the fermentation bacteria seed liquid is changed to a galactose yeast-like bacteria fermentation bacteria seed liquid.

[0042] Example 3 The method is generally the same as Example 1, except that in step a, the fermentation medium contains 20 g of glucose, 25 g of jujube fruit powder, 30 g of wolfberry fruit powder, 6 g of Centella asiatica powder per 1 kg, and the balance is water.

[0043] Example 4 It is generally the same as Example 1, except that in step a, the fermentation medium contains 20 g of glucose, 15 g of jujube fruit powder, 40 g of wolfberry fruit powder, 10 g of Centella asiatica powder per 1 kg, and the balance is water.

[0044] Example 5 The method is generally the same as Example 1, except that in step a, the fermentation medium contains 20 g of glucose, 20 g of jujube fruit powder, 20 g of wolfberry fruit powder, 5 g of Centella asiatica powder per 1 kg, and the balance is water.

[0045] Example 6 It is generally the same as Example 1, except that step a is changed to prepare a fermentation medium by mixing 20g of glucose, 20g of jujube fruit powder, 20g of wolfberry fruit powder, 5g of Centella asiatica powder and 822g of water, and then inoculating the fermentation culture medium with the seed liquid of galactosaccharomyces cerevisiae-like bacteria. The fermentation is carried out at a constant temperature of 30°C. When the fermentation time is 0-6h, the speed is set to 50 rpm; when the fermentation time is 6-9h, the speed is set to 100 rpm; when the fermentation time is 9-16h, the dissolved oxygen content of the fermentation tank is set to 20%, the stirring speed and the ventilation volume are linked to the dissolved oxygen content, and the speed and ventilation volume are automatically compensated and adjusted; when the fermentation time is 16-48h, the speed is set to 80 rpm; when the fermentation is completed at 48h, the stirring is stopped.

[0046] Comparative Example 1 A soothing and moisturizing composition is prepared by the following steps: Mix 2g of jujube fruit powder, 20g of wolfberry fruit powder, 5g of Centella asiatica powder per 1kg, and the balance is water, and extract with stirring at 60°C for 3h. After the extraction is completed, filter, centrifuge the filtrate, and take the supernatant to obtain a soothing and moisturizing composition.

[0047] Comparative Example 2 A soothing and moisturizing composition is prepared by the following steps: Step a: preparing a fermentation medium in a ratio of 20 g of glucose, 60 g of jujube powder and the balance of water per kg, then inoculating the fermentation culture medium with a saccharomyces cerevisiae fermentation strain seed solution, and fermenting at a constant temperature of 30° C. When fermenting for 0-6 hours, the rotation speed is set to 50 rpm; when fermenting for 6-9 hours, the rotation speed is set to 100 rpm; when fermenting for 9-16 hours, the dissolved oxygen content value of the fermentation tank is set to 20%, the stirring speed and the ventilation volume are linked to the dissolved oxygen content, and the speed and ventilation volume are automatically compensated and adjusted; when fermenting for 16-48 hours, the rotation speed is set to 80 rpm; when fermentation is completed at 48 hours, stirring is stopped; Step b: preparing a fermentation medium in a ratio of 20 g of glucose and 60 g of wolfberry powder per kg and the balance of water, then inoculating the fermentation culture medium with a saccharomyces cerevisiae fermentation strain seed solution, and fermenting at a constant temperature of 30° C. When fermenting for 0-6 h, the rotation speed is set to 50 rpm; when fermenting for 6-9 h, the rotation speed is set to 100 rpm; when fermenting for 9-16 h, the dissolved oxygen content value of the fermentation tank is set to 20%, the stirring speed and the ventilation volume are associated with the dissolved oxygen content, and the speed and ventilation volume are automatically compensated and adjusted; when fermenting for 16-48 h, the rotation speed is set to 80 rpm; when fermentation is completed at 48 h, stirring is stopped; Step c: preparing a fermentation medium in a ratio of 20 g of glucose and 15 g of Centella asiatica powder per kg, with the remainder being water, and then inoculating the fermentation culture medium with a seed solution of Saccharomyces cerevisiae. The fermentation is carried out at a constant temperature of 30° C. When the fermentation time is 0-6 h, the rotation speed is set to 50 rpm; when the fermentation time is 6-9 h, the rotation speed is set to 100 rpm; when the fermentation time is 9-16 h, the dissolved oxygen content value of the fermentation tank is set to 20%, the stirring speed and the ventilation volume are linked to the dissolved oxygen content, and the speed and ventilation volume are automatically compensated and adjusted; when the fermentation time is 16-48 h, the rotation speed is set to 80 rpm; when the fermentation time is 48 h, the stirring is stopped; Step 4: Separate the bacterial cells and fermentation culture medium of steps a, b, and c respectively by using a centrifuge at a speed of 15,000 rpm, collect the fermentation supernatant after centrifugation, and then sterilize the fermentation supernatant at 105°C for 30 min. After sterilization and cooling to room temperature, clarify and purify, and mix to obtain a soothing and moisturizing composition.

[0048] Comparative Example 3 It is generally the same as Example 5, except that in step a, the fermentation medium contains 20 g of glucose, 20 g of red ganoderma powder, 20 g of wolfberry powder, 5 g of Centella asiatica powder per 1 kg, and the balance is water.

[0049] Comparative Example 4 It is generally the same as Example 5, except that in step a, the fermentation medium contains 20 g of glucose, 20 g of jujube fruit powder, 20 g of oat powder, 5 g of Centella asiatica powder per 1 kg, and the balance is water.

[0050] Comparative Example 5 It is generally the same as Example 5, except that in step a, the fermentation medium contains 20 g of glucose, 20 g of jujube fruit powder, 20 g of wolfberry fruit powder, 5 g of gentian powder per 1 kg, and the balance is water.

[0051] Comparative Example 6 It is generally the same as Example 5, except that in step a, the fermentation medium contains 20 g of glucose, 28 g of wolfberry powder, 7 g of Centella asiatica powder per 1 kg, and the balance is water.

[0052] Comparative Example 7 It is generally the same as Example 5, except that in step a, the fermentation medium contains 20 g of glucose, 32 g of jujube fruit powder, 13 g of Centella asiatica powder per 1 kg, and the balance is water.

[0053] Comparative Example 8 It is generally the same as Example 5, except that in step a, the fermentation medium contains 20 g of glucose, 22 g of jujube fruit powder, 23 g of wolfberry fruit powder per 1 kg, and the balance is water.

[0054] Comparative Example 9 A soothing and moisturizing composition is prepared by the following steps: Mix 60g of jujube fruit powder per 1kg of jujube fruit powder and the rest of water, stir and extract at 60°C for 3h, filter after the extraction is completed, centrifuge the filtrate, and take the supernatant to obtain the jujube fruit extract; Mix 60 g of wolfberry powder per 1 kg of wolfberry powder and the rest of water, stir and extract at 60° C. for 3 h, filter after the extraction is completed, centrifuge the filtrate, and take the supernatant to obtain the wolfberry extract; Mix 15 g of Centella asiatica powder per 1 kg of Centella asiatica powder and the rest of water, stir and extract at 60°C for 3 hours, filter after the extraction is completed, centrifuge the filtrate, and take the supernatant to obtain the Centella asiatica extract; The jujube fruit extract, wolfberry fruit extract and Centella asiatica extract are mixed to obtain a soothing and moisturizing composition.

[0055] Efficacy Test 1. Soothing effect test Hyaluronidase inhibition rate 1. Purpose and principle of the experiment Hyaluronidase is a specific cleavage enzyme of hyaluronic acid, a participant in allergic reactions, and has a strong correlation with the release of histamine by mast cells. Whether the test sample has a soothing effect can be judged by the hyaluronidase inhibition rate. The higher the hyaluronidase inhibition rate, the stronger the soothing effect of the substance, and vice versa.

[0056] This test refers to the laboratory method (HMC-WI-029 hyaluronidase inhibition rate) and compares the hyaluronidase inhibition rate test results of the test sample with that of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain soothing effect.

[0057] This test method is an in vitro method suitable for evaluating cosmetic products that claim to achieve soothing effects by inhibiting hyaluronidase.

[0058] 2 Test indicators Hyaluronidase inhibition rate determination criteria: If the hyaluronidase inhibition rate of the sample is higher than that of the negative control and there is a significant difference (P<0.05), then it can be considered that the test sample has a certain soothing effect.

[0059] 3 Experimental materials and methods 3.1 Instruments and equipment: BSA224S analytical balance, L6s UV spectrophotometer; 3.2 Reagents: Hyaluronidase BR, Sodium Hyaluronate BR; 3.3 Test methods (1) Treatment of control and test samples Sample group: 10% concentration of Examples 1-6 and Comparative Examples 1-9; Positive control (dipotassium glycyrrhizinate, purity ≥98%): diluted with water to a positive control concentration of 3%; Negative control: pure water.

[0060] (2) Test operation steps Set up a sample group, a sample background group, a solvent group and a solvent background group. Each group needs to have 3 parallels. Add different reagent solutions to the four groups respectively, shake well, and leave at room temperature for 30 minutes for color development. Use a UV spectrophotometer to measure the absorbance value at a wavelength of 528nm.

[0061] (3) Calculation formula: .

[0062] Where: A—is the absorbance of the reaction solution without sample; B—is the absorbance of the reaction solution without sample and enzyme; C—is the absorbance of the reaction solution containing sample and enzyme; D—is the absorbance of the reaction solution containing sample and without enzyme.

[0063] (4) Data analysis SPSS was used as the statistical analysis software, and the independent sample t-test was used to compare the hyaluronidase inhibition rates among the test samples, positive controls, and negative controls.

[0064] The above statistical analyses were all two-tailed tests, with a significance level of α = 0.05. P>0.05 means there is no significant difference between the two groups; P<0.05 means there is a significant difference between the two groups.

[0065] The compositions obtained in Examples 1-6 and Comparative Examples 1-9 were tested according to the above test method, and the jujube fruit extract, Centella asiatica extract, and Lycium barbarum fruit extract obtained in the preparation process of Comparative Example 2 were tested. The results are shown in Table 1. Table 1 Test results of soothing efficacy of the compositions obtained in Examples 1-6 and Comparative Examples 1-9 Hyaluronidase inhibition rate% P-value Example 1 74.628 ＜0.050 Example 2 75.193 ＜0.050 Example 3 79.370 ＜0.050 Example 4 79.569 ＜0.050 Example 5 77.542 ＜0.050 Example 6 78.100 ＜0.050 Comparative Example 1 59.926 ＜0.050 Comparative Example 2 65.174 ＜0.050 Comparative Example 3 52.110 ＜0.050 Comparative Example 4 51.273 ＜0.050 Comparative Example 5 58.545 ＜0.050 Comparative Example 6 60.448 ＜0.050 Comparative Example 7 39.875 ＜0.050 Comparative Example 8 68.059 ＜0.050 Comparative Example 9 59.763 ＜0.050 Negative control -2.66 / Positive Control 62.48 ＜0.050 Jujube Fruit Extract 35.990 ＜0.050 Centella Asiatica Extract 17.391 ＜0.050 Lycium barbarum fruit extract 58.213 ＜0.050

[0066] According to the results in Table 1, we can see that: According to the data of Examples 1-6, the technical solution of the present invention can achieve extremely excellent skin soothing effect; Furthermore, according to the comparison between Example 1 and Example 2 and Example 6, the technical scheme of the present invention using galactose yeast-like bacteria to carry out composite fermentation of jujube fruit, wolfberry fruit and Centella asiatica is better than the technical scheme of using brewer's yeast to carry out composite fermentation of jujube fruit, wolfberry fruit and Centella asiatica, and the soothing effect of the composition is improved, indicating that galactose yeast-like bacteria is more effective in the composite fermentation of jujube fruit, wolfberry fruit and Centella asiatica.

[0067] According to the data comparison of the extracts fermented separately in each group in Example 5 and Comparative Example 2, it can be seen that the technical solution of obtaining jujube fruit extract, wolfberry fruit extract and Centella asiatica extract by fermenting them separately and then combining them in Comparative Example 2 only plays a simple effect superposition, and does not give full play to the soothing effect of jujube fruit extract, wolfberry fruit extract and Centella asiatica extract; based on the hyaluronidase inhibition rate of jujube fruit extract, wolfberry fruit extract and Centella asiatica extract respectively, we can know that Comparative Example 2 conforms to the conventional law of effect superposition, because when the hyaluronidase inhibition rate reaches a certain level, the difficulty of improving it will become greater and greater, and Comparative Example 2 uses the combination of jujube fruit extract, wolfberry fruit extract and Centella asiatica extract. Although it improves the skin soothing effect to a certain extent, compared with Example 5 of the present invention, the skin soothing effect of Example 5 is significantly improved, indicating that in the process of co-fermentation of jujube fruit, wolfberry fruit and Centella asiatica, a synergistic effect may be exerted, the fermentation effect is improved, and the soothing effect of jujube fruit extract, wolfberry fruit extract and Centella asiatica extract is fully exerted.

[0068] In this regard, the applicant verified the extraction methods of active ingredients from jujube fruit, wolfberry fruit and Centella asiatica, and found that the effects of Comparative Example 1 and Comparative Example 9, whether mixed extraction or separate extraction combination, were almost the same as those of Comparative Example 2, further verifying that the fermentation effect was synergistically improved during the co-fermentation of jujube fruit, wolfberry fruit and Centella asiatica.

[0069] According to the data comparison of Example 5 and Comparative Examples 3-5, the technical solution of using Ganoderma lucidum instead of jujube fruit, oats instead of wolfberry fruit, and gentian instead of Centella asiatica does not show a significant improvement in soothing effect like the technical solution of the present invention. It can be confirmed that the technical solution of the present invention using jujube fruit, wolfberry fruit and Centella asiatica as fermentation substrates for co-fermentation has a synergistic effect of improving the skin soothing effect.

[0070] Similarly, according to the data comparison of Example 5 and Comparative Examples 6-8, in the absence of any one of jujube fruit, wolfberry fruit and Centella asiatica, the hyaluronidase inhibition rates of Comparative Examples 6-8 were significantly reduced, indicating that the synergistic effect of enhancing the fermentation effect and thus enhancing the skin soothing effect of the present invention is based on the synergistic fermentation of jujube fruit, wolfberry fruit and Centella asiatica.

[0071] 2. Antioxidant efficacy test DPPH free radical scavenging rate 1. Purpose and principle of the experiment Excessive production of free radicals can lead to natural aging and photoaging of the skin, thus causing wrinkles. Therefore, whether it has the ability to scavenge free radicals is one of the important indicators for evaluating anti-aging and anti-oxidant cosmetics (raw materials).

[0072] This test refers to the laboratory method (HMC-WI-030 DPPH free radical scavenging rate) and compares the DPPH free radical scavenging rate test results of the test sample with that of the negative control. If the scavenging rate of the test sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain antioxidant effect.

[0073] This test method is an in vitro method and is suitable for evaluating cosmetic products that claim to achieve antioxidant effects by scavenging free radicals.

[0074] 2 Test indicators DPPH free radical scavenging rate determination standard: If the DPPH scavenging rate of the sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain antioxidant effect.

[0075] 3 Experimental materials and methods 3.1 Instruments and equipment: BSA224S analytical balance, L6s UV spectrophotometer; 3.2 Reagents: DPPH (1,1-diphenyl-2-picrylhydrazyl), 98%; 3.3 Test methods (1) Treatment of control and test samples Sample group: 100% of Examples 1-6 and Comparative Examples 1-9; Positive control (vitamin E, purity ≥96%): dilute with 95% ethanol to a positive control concentration of 0.1%; Negative control: pure water.

[0076] (2) Test operation steps Set up sample tubes, sample background tubes, DPPH tubes, and solvent background tubes. Set up 3 parallel tubes for each group. Add different reagent solutions to the four groups, shake gently, and let stand at room temperature for 5 minutes. Transfer the reaction solutions of each group into a 1 cm cuvette and measure the absorbance at 517 nm.

[0077] (3) Calculation formula: .

[0078] Where: T—absorbance of sample tube, i.e. absorbance of solution after sample reacts with DPPH; T0—sample background absorbance; C—the average value of the absorbance of the DPPH tube three times, that is, the absorbance of the DPPH solution when no sample is added; C0—Solvent background absorbance.

[0079] The compositions of Examples 1-6 and Comparative Examples 1-9 were tested and calculated according to the above method, and the jujube fruit extract, Centella asiatica extract, and Lycium barbarum fruit extract obtained in the preparation process of Comparative Example 2 were tested, and the results are shown in Table 2; Table 2 Antioxidant efficacy results of the compositions of Examples 1-6 and Comparative Examples 1-9 DPPH free radical scavenging rate% P-value Example 1 40.254 ＜0.050 Example 2 40.499 ＜0.050 Example 3 42.137 ＜0.050 Example 4 42.393 ＜0.050 Example 5 41.662 ＜0.050 Example 6 41.867 ＜0.050 Comparative Example 1 28.040 ＜0.050 Comparative Example 2 26.497 ＜0.050 Comparative Example 3 21.923 ＜0.050 Comparative Example 4 21.034 ＜0.050 Comparative Example 5 20.364 ＜0.050 Comparative Example 6 25.606 ＜0.050 Comparative Example 7 8.411 ＜0.050 Comparative Example 8 29.330 ＜0.050 Comparative Example 9 28.102 ＜0.050 Positive control group 93.880 ＜0.050 Negative control group -0.382 / Jujube Fruit Extract 7.623 ＜0.050 Centella Asiatica Extract -0.804 ＞0.050 Lycium barbarum fruit extract 21.490 ＜0.050

[0080] According to the results in Table 2: According to the data of Examples 1-6, the soothing and moisturizing composition of the present invention has a relatively excellent antioxidant effect.

[0081] According to the data comparison of Example 5 and Comparative Example 2, and each group of extracts fermented separately in Comparative Example 2, it can be seen that the technical scheme of Comparative Example 2, which uses separate fermentation to obtain jujube fruit extract, wolfberry fruit extract and Centella asiatica extract and then combines them, only plays a simple effect superposition, and does not give full play to the antioxidant effect of jujube fruit extract, wolfberry fruit extract and Centella asiatica extract; based on the DPPH free radical scavenging rate of each of jujube fruit extract, wolfberry fruit extract and Centella asiatica extract, we can know that the Centella asiatica extract fermented alone has no antioxidant effect, and the combination of jujube fruit extract, wolfberry fruit extract and Centella asiatica extract in Comparative Example 2 only produces a simple effect superposition. In contrast, in Example 5 of the present invention, on the basis of the co-fermentation of jujube fruit, wolfberry fruit and Centella asiatica, the DPPH free radical scavenging rate achieved is significantly improved, indicating that the technical scheme of the present invention using jujube fruit, wolfberry fruit and Centella asiatica as fermentation substrates for fermentation produces a synergistic effect of improving the fermentation effect, thereby improving the antioxidant effect of the composition.

[0082] Furthermore, the applicant verified the synergistic effect of Comparative Examples 1 and 9 by changing the extraction method, and found that there was no obvious difference in the antioxidant efficacy of the composition obtained by composite extraction and single extraction using the conventional extraction method rather than the biological fermentation method, indicating that the synergistic effect of the present invention is produced by the biological fermentation technology combined with the co-fermentation of jujube fruit, wolfberry fruit and Centella asiatica; and according to the data comparison of Comparative Examples 1, 9 and 2, it can be seen that the plant extraction method should obtain more antioxidant active ingredients or the antioxidant effect that can be exerted is more direct, compared with the technical solution of fermenting jujube fruit, wolfberry fruit and Centella asiatica separately and then combining them, so that the DPPH free radical scavenging rate shown in Comparative Examples 1 and 9 is higher than that of Comparative Example 2.

[0083] According to the data comparison of Example 5 and Comparative Examples 3-5, the technical solution of using Ganoderma lucidum instead of jujube fruit, oats instead of wolfberry fruit, and gentian instead of Centella asiatica does not show a significant improvement in antioxidant efficacy like the technical solution of the present invention. It can be confirmed that the technical solution of the present invention using jujube fruit, wolfberry fruit and Centella asiatica as fermentation substrates for co-fermentation has the effect of synergistically improving the antioxidant effect of the skin.

[0084] Similarly, according to the data comparison of Example 5 and Comparative Examples 6-8, in the absence of any one of jujube fruit, wolfberry fruit and Centella asiatica, the DPPH radical scavenging rates of Comparative Examples 6-8 were significantly reduced, indicating that the synergistic effect of enhancing the fermentation effect and thus enhancing the antioxidant effect of the skin of the present invention is based on the synergistic fermentation of jujube fruit, wolfberry fruit and Centella asiatica.

[0085] 3. Moisturizing effect test Zebrafish tail fin length test 1. Experimental purpose and principle When zebrafish is treated with sodium chloride, the skin surface will lose water and shrink due to osmotic pressure, and the tail will become smaller due to shrinkage. The moisturizing effect of cosmetics can be evaluated by the impact on the tail.

[0086] This test method is an in vitro method and is suitable for evaluating the moisturizing effect of cosmetics.

[0087] 2 Test indicators Criteria for judging the length of zebrafish tail fin: The length of the zebrafish tail fin of the test sample was greater than that of the model group, and there was a significant difference (P<0.05), indicating that the sample had a moisturizing effect.

[0088] 3 Experimental materials and methods 3.1 Instruments and equipment: BSA224S analytical balance, stereo microscope.

[0089] 3.2 Reagents: Sodium bicarbonate (NaHCO3); Potassium chloride (KCl); Calcium chloride (CaCl2·2H2O); Magnesium sulfate (MgSO4·7H2O); Sodium chloride (NaCl); Dimethyl sulfoxide (C2H6OS); Sodium hyaluronate.

[0090] 3.3 Test methods (1) Treatment of control and test samples Blank group: standard dilution water; Model group: sodium chloride solution; Positive group: sodium chloride solution + sodium hyaluronate; Sample group: Diluted with sodium chloride solution to a concentration of 2% for the composition of Examples 1-6 and Comparative Examples 1-9.

[0091] (2) Test operation steps Normally developed embryos were randomly selected for the experiment, and a 12-well cell culture plate was used as the experimental carrier. The prepared test sample dilutions of different concentrations were added to each well of the 12-well cell culture plate, and the plate was covered and then placed in a constant temperature box for static. Three parallel groups were set for each concentration group, and a blank group, a model group, and a positive group were also set.

[0092] (3) Calculation formula: .

[0093] (4) Data analysis The statistical analysis software was GraphPad Prism, and the independent sample t test was used to compare the caudal fin length of zebrafish in the sample group, blank group, positive group and model group. The above statistical analyses were all two-tailed tests, and the significance level was α=0.05. P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0094] The compositions of Examples 1-6 and Comparative Examples 1-9 were tested according to the above test method, and the results are shown in Table 3; Table 3 Moisturizing effect test results of the compositions of Examples 1-6 and Comparative Examples 1-9 Average tail fin length Relative moisturizing effect% P-value Blank group 440 / ＜0.050 Model Group 412.8 / / Positive group 439.8 98.85 ＜0.050 Example 1 431.3 68.01 ＜0.050 Example 2 431.7 69.49 ＜0.050 Example 3 433.7 76.84 ＜0.050 Example 4 433.9 77.57 ＜0.050 Example 5 432.8 73.43 ＜0.050 Example 6 433.5 76.12 ＜0.050 Comparative Example 1 427.5 54.04 ＜0.050 Comparative Example 2 427.9 55.51 ＜0.050 Comparative Example 3 423.8 40.44 ＜0.050 Comparative Example 4 427.3 53.31 ＜0.050 Comparative Example 5 426.2 49.26 ＜0.050 Comparative Example 6 422.3 34.93 ＜0.050 Comparative Example 7 424.4 42.65 ＜0.050 Comparative Example 8 425.1 45.22 ＜0.050 Comparative Example 9 428.0 55.88 ＜0.050 Jujube Fruit Extract 418.8 22.06 ＜0.050 Centella Asiatica Extract 416.8 14.71 ＜0.050 Lycium barbarum fruit extract 417.5 17.28 ＜0.050

[0095] According to the data in Table 3: According to the data of Examples 1-6, the technical solution of the present invention can achieve extremely excellent skin moisturizing effect.

[0096] According to the data comparison of the extracts fermented separately in each group in Example 5 and Comparative Example 2, it can be seen that the technical solution of obtaining jujube fruit extract, wolfberry fruit extract and Centella asiatica extract by fermenting them separately and then combining them in Comparative Example 2 only plays a simple effect superposition, and does not give full play to the moisturizing effect of jujube fruit extract, wolfberry fruit extract and Centella asiatica extract; based on the relative moisturizing effects of jujube fruit extract, wolfberry fruit extract and Centella asiatica extract, we can know that Comparative Example 2 conforms to the conventional law of effect superposition, because when the relative moisturizing effect reaches a certain level, the difficulty of improving it will become greater and greater, and Comparative Example 2 uses the combination of jujube fruit extract, wolfberry fruit extract and Centella asiatica extract, although it improves the skin moisturizing effect to a certain extent, compared with Example 5 of the present invention, the skin moisturizing effect of Example 5 is significantly improved, indicating that in the process of co-fermentation of jujube fruit, wolfberry fruit and Centella asiatica, a synergistic effect may be exerted to improve the fermentation effect, thereby giving full play to the moisturizing effect of jujube fruit extract, wolfberry fruit extract and Centella asiatica extract.

[0097] In this regard, the applicant verified the extraction methods of active ingredients from jujube fruit, wolfberry fruit and Centella asiatica, and found that the effects of Comparative Example 1 and Comparative Example 9, whether mixed extraction or separate extraction combination, were almost the same as those of Comparative Example 2, further verifying that the fermentation effect was synergistically improved during the co-fermentation of jujube fruit, wolfberry fruit and Centella asiatica.

[0098] According to the data comparison of Example 5 and Comparative Examples 3-5, the technical solution of using Ganoderma lucidum instead of jujube fruit, oats instead of wolfberry fruit, and gentian instead of Centella asiatica does not show a significant improvement in moisturizing effect like the technical solution of the present invention. It can be confirmed that the technical solution of the present invention using jujube fruit, wolfberry fruit and Centella asiatica as fermentation substrates for co-fermentation has the effect of synergistically improving the skin moisturizing effect.

[0099] Similarly, according to the data comparison of Example 5 and Comparative Examples 6-8, in the absence of any one of jujube fruit, wolfberry fruit and Centella asiatica, the relative moisturizing effects of Comparative Examples 6-8 are significantly reduced, indicating that the synergistic effect of enhancing the fermentation effect and thus enhancing the skin moisturizing effect of the present invention is based on the synergistic fermentation of jujube fruit, wolfberry fruit and Centella asiatica.

[0100] 4. Irritation Test Safety Testing Test method: Chicken embryo chorioallantoic membrane test 1. Purpose and principle of the test The chicken embryo chorioallantoic membrane test is an early in vitro eye irritation assessment method. The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chicken embryo. This test uses the characteristics of the intact, clear and transparent vascular system of the chorioallantoic membrane of the mid-stage incubated chicken embryo. A certain amount of the test substance is directly exposed to the chicken embryo chorioallantoic membrane. After a period of exposure, the changes in the toxic effect indicators of the chorioallantoic membrane (such as bleeding, coagulation and vascular melting) are observed. These indicators reflect the changes in the morphological structure, color and permeability of the blood vessels and vascular networks, as well as the phenomena such as the denaturation of the chorioallantoic membrane proteins and the degree of damage. Then a score is obtained by combining them to evaluate the eye irritation of the test substance.

[0101] The purpose of this test is to test the ability of the test substance to cause toxic changes in the chicken embryo chorioallantoic membrane and to evaluate the elements and processes of the potential eye irritation of the evaluated substance.

[0102] 2. Experimental Group Treatment and Reagents Sample group: the soothing and moisturizing compositions prepared in Examples 1-6 and Comparative Examples 1-9.

[0103] Negative control: 0.9% saline.

[0104] Positive control: 1% sodium dodecyl sulfate (SDS) solution.

[0105] 3. Experimental steps In this test, 6 chicken embryos were selected for each group, and the conditions of the chorioallantoic membrane were recorded with a camera. A polytetrafluoroethylene resin ring was placed on the chorioallantoic membrane of the chicken embryo and photographed. The sample to be tested was added to the polytetrafluoroethylene resin ring, the time of adding the sample was recorded, and the air chamber was covered with a moistened plastic wrap. The chicken embryo was moved to a constant temperature and humidity chamber for culture, and the degree of change of each toxic effect was observed.

[0106] 4. Calculation of results The endpoint evaluation method was used to calculate the endpoint evaluation (ES) with the result retained to two decimal places; each chicken embryo was scored as the sum of the observed bleeding, coagulation and vascular melting degrees for each chicken embryo; the average value of the mathematical sum obtained for ES-6 chicken embryos was taken.

[0107] The average ES score is calculated according to the following formula: .

[0108] 5. Result judgment criteria ES≤4, non-irritating; 4<ES≤12, mild irritation; 12<ES<16, moderate irritation; ES ≥ 16, strong irritant / corrosive.

[0109] 2. Test Results According to the above test method, samples of Examples 1-6 and Comparative Examples 1-9 were tested, and the ES was less than or equal to 4, and the results were all judged to be non-irritating.

[0110] Among them, the results of chicken embryos before and after use in Example 6 are as follows Figure 1-Figure 2 As shown, the sample test result of Example 6 is ES=2.67; This proves that the technical solution of the present invention is mild and non-irritating.

[0111] Application Example 1 On the basis of the composition prepared in Example 6, glycerol, 1,2-hexanediol and p-hydroxyacetophenone were added to obtain a beauty liquid. The specific preparation steps are as follows: 1. Pre-preparation: Dissolve glycerol, p-hydroxyacetophenone and 1,2-hexanediol in advance and stir to mix evenly; 2. Main pot: add the soothing and moisturizing composition of Example 6 and stir to mix evenly.

[0112] The specific components of Application Example 1 are shown in Table 4; Table 4 Composition of beauty serum Components Addition amount (%) The soothing and moisturizing composition of Example 6 88.7 glycerin 10 1,2-Hexanediol 0.8 p-Hydroxyacetophenone 0.5

[0113] Application Example 2 A soothing and moisturizing essence, the specific formula is shown in Table 5, and the preparation method is: 1. Pre-prepared phase: Dissolve phase B in advance and stir to mix evenly; Mix phase C in advance and stir to dissolve, set aside.

[0114] 2. Main pot: Add the pure water in phase A into the pot, start homogenization, add the remaining ingredients in phase A in sequence, and homogenize at medium speed for 60 seconds. Stir and heat to 85°C, homogenize for 2 minutes, and there are no gel particles; keep warm for 30 minutes, then stir and cool down.

[0115] 3. Cool down to 50℃, add phase B and phase C in sequence and stir evenly.

[0116] 4. Cool down to 45°C, add phase D and stir well.

[0117] Table 5 Soothing and moisturizing essence formula

[0118] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. A soothing and moisturizing composition, characterized in that: The product is obtained by fermenting jujube fruit, wolfberry fruit and Centella asiatica with yeast and / or galactosaccharomyces-like bacteria in the fermentation substrate. The weight ratio of the jujube fruit, wolfberry fruit and Centella asiatica is 1-5:2-8:1-2.

2. The soothing and moisturizing composition according to claim 1, characterized in that: The yeast is one of Saccharomyces cerevisiae, Pichia pastoris and Kluyveromyces cerevisiae.

3. A method for preparing the soothing and moisturizing composition according to claim 1, characterized in that: The steps include: Step 1: performing primary culture on yeast and / or galactosaccharomyces-like bacteria to obtain a fermentation strain activation liquid; Step 2: inoculating the fermentation strain activation liquid for secondary culture to obtain the fermentation strain seed liquid; Step 3: using jujube fruit, wolfberry fruit and Centella asiatica as fermentation substrates to prepare a fermentation medium, then inoculating the fermentation bacteria seed liquid for fermentation, and separating to obtain the soothing and moisturizing composition after the fermentation is completed.

4. The method for preparing the soothing and moisturizing composition according to claim 3, characterized in that: The specific operation of step 3 is: prepare a fermentation medium by mixing 20g of glucose, 5-25g of jujube fruit powder, 10-40g of wolfberry fruit powder, 5-10g of Centella asiatica powder per kg, with the remainder being water, then inoculate the fermentation bacteria seed liquid for fermentation, and after the fermentation is completed, separate to obtain the soothing and moisturizing composition.

5. The method for preparing the soothing and moisturizing composition according to claim 4, characterized in that: The specific operation of inoculating the fermentation bacteria seed liquid for fermentation is as follows: fermentation at a constant temperature of 30°C, and when fermenting for 0-6h, set the speed to 50 rpm; when fermenting for 6-9h, set the speed to 100 rpm; when fermenting for 9-16h, set the dissolved oxygen content value of the fermentation tank to 20%, and the stirring speed and ventilation volume are linked to the dissolved oxygen content, and the speed and ventilation volume are automatically compensated and adjusted; when fermenting for 16-48h, set the speed to 80 rpm; when the fermentation is completed at 48h, stop stirring.

6. Use of the soothing and moisturizing composition according to claim 1 or 2 in preparing cosmetics.

7. A skin care product, characterized in that: Contains 0.01-50wt% of the soothing and moisturizing composition as claimed in claim 1 or 2.

8. The skin care product according to claim 7, characterized in that The skin care product is one of an aqueous solution, an essence, an emulsion, and a cream.

9. A beauty liquid, characterized in that: The invention comprises 83.2-89.1 wt % of the soothing and moisturizing composition according to claim 1, and further comprises 10-15 wt % of glycerin, 0.5-1 wt % of 1,2-hexanediol and 0.4-0.8 wt % of p-hydroxyacetophenone.

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