A fully automatic drug detection instrument and automatic detection method

Abstract

The present invention relates to the technical field of drug detection devices, and discloses a fully automatic drug detection instrument and an automatic detection method, which includes an outer box, an oscillating mechanism, a heating mechanism, a reagent tank, an optical detection mechanism, a moving mechanism, a pipette holder and a microplate holder are arranged inside the outer box, the reagent tank is used to store detection samples and detection reagents, the pipette is used to extract detection samples and detection reagents, the microplate holder is used to place the microplate for processing detection samples, the moving mechanism is used to move the microplate and the pipette, the optical detection mechanism is used to test the absorbance of the detection sample, the reagent tank is located in the middle position of the bottom of the outer box, the pipette holder and the microplate holder are respectively located on both sides of the reagent tank, the optical detection mechanism and the oscillating mechanism are respectively located on the other two sides of the reagent tank, and the heating mechanism is located on the side of the oscillating mechanism close to the microplate holder. The present invention can alleviate the problem of low detection efficiency of biological preparations such as drugs.

Description

Technical Field

[0001] The present invention relates to the field of drug detection devices, and in particular to a fully automatic drug detection instrument and an automatic detection method. Background Art

[0002] The detection of biological agents such as drugs can be used to evaluate biological agents. For example, through the detection of heparin, the potency of heparin and the activity index can be obtained, which is of great significance to the application of biological agents.

[0003] At present, the testing of biological agents, such as heparin potency, has relatively complicated steps and it is even more difficult to achieve automatic testing. Existing testing equipment operates through robotic arms under the coordination of instructions, but has a high demand for manual cooperation and cannot automate the entire testing process, resulting in low testing efficiency. Summary of the invention

[0004] In order to alleviate the problem of low detection efficiency of biological preparations such as drugs, the present invention provides a fully automatic drug detection instrument.

[0005] The present invention provides a fully automatic drug detection instrument, which adopts the following technical solution:

[0006] A fully automatic drug detection instrument comprises an outer box body, wherein an oscillating mechanism, a heating mechanism, a reagent tank, an optical detection mechanism, a moving mechanism, a pipette holder and a microplate holder are arranged inside the outer box body, wherein the reagent tank is used to store detection samples and detection reagents, the pipette holder is used to place a pipette for transferring reagents, the pipette is used to extract detection samples and detection reagents, the microplate holder is used to place a microplate for processing detection samples, the moving mechanism is used to move the microplate and the pipette, the oscillating mechanism is used to oscillate the microplate, the heating mechanism is used to heat the reagents in the microplate, the optical detection mechanism is used to test the absorbance of the detection sample, the moving mechanism is connected to the inner top of the outer box body, the reagent tank is located in the middle of the bottom of the outer box body, the pipette holder and the microplate holder are respectively located on both sides of the reagent tank, the optical detection mechanism and the oscillating mechanism are respectively located on the other two sides of the reagent tank, and the heating mechanism is located on the side of the oscillating mechanism close to the microplate holder.

[0007] By adopting the above technical solution, an oscillation mechanism, a heating mechanism and an optical detection mechanism are integrated in the outer box, so that the whole process from biological sample processing to result detection can be completed on one device. The design of the moving mechanism enables the instrument to automatically move the microplate and the pipette, thereby reducing the steps and time of manual operation and improving the detection efficiency. Automated operation also helps to reduce human errors and improve the accuracy of the test results. In addition, the components inside the instrument are reasonably arranged according to the order of detection, so that the moving path of the moving mechanism is simpler and the problem of repeated movement of the moving mechanism is reduced.

[0008] Optionally, a collection box is provided between the pipette holder and the oscillating mechanism, and the collection box is used to collect used pipettes.

[0009] By adopting the above technical solution, a collection box for recovering pipettes is arranged between the pipette holder and the oscillating mechanism. After adding detection reagents or detection samples to the microplate on the oscillating mechanism using the pipette, the used pipette is moved to the top of the collection box under the drive of the moving mechanism, the moving mechanism is separated from the pipette so that it falls into the collection box, and after the moving mechanism is separated from the pipette, it is moved to the top of the pipette holder to assemble unused pipettes, thereby further optimizing the moving path of the moving mechanism and improving the detection efficiency.

[0010] Optionally, the moving mechanism includes a mounting seat, a pipetting assembly, a clamping assembly, a driving assembly, a first lifting assembly and a second lifting assembly, the driving assembly is connected to the outer box body, the mounting seat is connected to the driving assembly, the driving assembly drives the mounting seat to move in a plane parallel to the bottom surface of the outer box body, the pipetting assembly is connected to the mounting seat through the first lifting assembly, the first lifting assembly drives the pipetting assembly to move in a direction perpendicular to the ground surface of the outer box body, the pipetting assembly is detachably connected to the pipette, the clamping assembly is connected to the mounting seat through the second lifting member, the second lifting assembly drives the clamping assembly to move in a direction perpendicular to the ground surface of the outer box body, and the clamping assembly is used to clamp the microplate.

[0011] Optionally, the pipette assembly includes a pipette and a pipette gun, the pipette gun includes a plurality of connecting nozzles for connecting to the pipette, the connecting nozzles are hollow tube structures, two sealing rings are provided on the outer wall of the connecting nozzles, the two sealing rings are arranged along the axial direction of the connecting nozzles, and the connecting nozzles are interference fit with the tube mouth of the pipette.

[0012] By adopting the above technical solution, two sealing rings are arranged on the outer wall of the connecting nozzle, so as to improve the sealing effect of the connection between the connecting nozzle and the pipette, and at the same time make the connection between the connecting nozzle and the pipette more stable, so that the liquid transmission during the pipetting process is more stable, and reduce the error caused by liquid leakage or loose connection.

[0013] Optionally, a separation plate is provided on the pipette gun, and a plurality of through holes are opened on the separation plate. The plurality of through holes are arranged in one-to-one correspondence with a plurality of connecting nozzles, and the connecting nozzles are inserted into the corresponding through holes. The separation plate is connected to the pipette gun through an electric push cylinder, and the electric push cylinder drives the separation plate to slide along the axial direction of the connecting nozzle.

[0014] By adopting the above technical solution, a separation plate is connected to the pipette, and the separation plate is connected to the pipette through an electric push cylinder. The connecting nozzle of the pipette is connected to the pipette after passing through the through hole opened on the connecting plate. When the pipette needs to be separated from the pipette after use, the electric push cylinder is used to drive the separation plate to move in the direction close to the pipette, so that the separation plate abuts against the end of the pipette, and the electric push cylinder continues to push the separation plate to move. The pipette is separated from the connecting nozzle of the pipette under the thrust of the separation plate, thereby improving the convenience of separating multiple pipettes from the connecting nozzle of the pipette at the same time and improving the detection efficiency.

[0015] Optionally, the clamping assembly includes a driving member and two clamping jaws, the driving member is connected to the second lifting assembly, the driving member drives the two clamping jaws to move towards or away from each other, and two conical protrusions are provided on the side where the two clamping jaws are close to each other, and the two conical protrusions are symmetrically arranged.

[0016] By adopting the above technical solution, the driving member drives the two jaws to move towards or away from each other, and the two jaws are used to clamp the microplate. Two conical protrusions are symmetrically arranged on the side where the two jaws are close to each other. When clamping the microplate, the four conical protrusions abut against the side wall of the microplate. The design of the conical protrusions increases the friction force when the jaws contact the microplate, making the clamping of the microplate more stable. At the same time, the symmetrical arrangement of the conical protrusions ensures the uniform distribution of the clamping force, avoiding damage to the object or unstable clamping due to uneven clamping force.

[0017] Optionally, an anti-volatile reagent kit is arranged in the outer box body, and the anti-volatile reagent kit is used to accommodate volatile detection reagents. The anti-volatile reagent kit includes a box body and a box cover, and the box body is used to accommodate volatile detection reagents. The box cover is hinged to the box body, and the box cover is used to close the box opening of the box body. A groove is provided on the side wall of the box cover, and the length direction of the groove is perpendicular to the rotation axis of the box cover. A toggle block for inserting into the groove is provided on the side wall of the clamp away from the conical protrusion.

[0018] By adopting the above technical solution, an anti-volatile reagent kit is arranged in the outer box body, and the anti-volatile reagent kit is used to contain the volatile detection reagent, which can effectively seal the reagent and prevent it from volatilizing into the air, thereby ensuring the purity and stability of the reagent and improving the accuracy of the detection. In addition, by providing a groove on the side wall of the box cover and providing a toggle block matching the groove on the clamp, when the box cover needs to be opened, the clamp can be inserted into the groove through the toggle block, and then the driving component drives the clamp to move, thereby driving the box cover to rotate and open, thereby improving the degree of automation of the detection device and making the experimental process smoother and more efficient.

[0019] Optionally, the pipette holder includes a holder body and a pipette placement plate, the pipette placement plate is provided with a plurality of placement holes for plugging in pipettes, the pipette placement plate is detachably connected to the holder body, the anti-evaporation reagent kit is located on one side of the pipette holder, and the pipette holder limits the opening angle of the box cover to less than 180 degrees.

[0020] By adopting the above technical solution, the pipette placement plate and the bracket body are connected in a detachable manner, so that the experimenter can adjust or replace the placement plate according to actual needs to adapt to pipettes of different specifications or quantities, thereby improving the flexibility and practicality of the equipment. In addition, by arranging the anti-volatile reagent kit on one side of the pipette bracket, the pipette bracket is used to limit the opening angle of the box cover to less than 180 degrees. When the detection reagent in the anti-volatile reagent kit is taken out, the toggle block on the clamp is used to push the box cover to rotate on the side of the box cover away from the box body, so that the box cover is buckled on the box body.

[0021] Optionally, the oscillation mechanism comprises a support tray for clamping the microplate, and spring discs are fixedly connected to any three inner walls of the support tray.

[0022] By adopting the above technical solution, the microplate is clamped using a support tray, and the microplate can be firmly fixed on the oscillation mechanism, avoiding experimental errors caused by loosening during the oscillation process. The spring disc has excellent elasticity and recovery, and can generate stable vibration waves during the oscillation process, so that the liquid or sample in the microplate can be evenly mixed. In addition, the spring disc is fixedly connected to any three inner walls of the support tray, so that the positioning of the microplate is more accurate, and the stability of the clamping assembly in clamping the microplate is improved.

[0023] The present invention also provides an automatic detection method of a fully automatic drug detection instrument, which adopts the following technical solution:

[0024] The automatic detection method is applied to the above-mentioned fully automatic drug detection instrument, and the automatic detection method comprises:

[0025] Configuring target position information for the pipette so that the pipette automatically moves according to the target position information;

[0026] Get real-time pipette position information;

[0027] When the pipette is at the operating point, a liquid level detection instruction is sent to the liquid level detection sensor, and current liquid level information is obtained;

[0028] Based on the current liquid level information, when the current liquid level information is inconsistent with the preset empty liquid level information, the vertical movement distance is calculated based on the current liquid level information, including: obtaining a first parameter of the corresponding container by looking up a table based on the current operation point position; when taking liquid, based on the current liquid taking amount, when the current liquid taking amount is greater than a first threshold, correcting the current liquid level information; based on the first parameter and the corrected liquid level information, calculating the vertical movement distance, and sending the movement distance to the pipette to control the depth of the pipette extending below the liquid surface.

[0029] In summary, the present invention includes at least one of the following beneficial technical effects:

[0030] 1. By integrating the oscillation mechanism, heating mechanism and optical detection mechanism in the outer box, the whole process from biological sample processing to result detection can be completed on one device. The design of the moving mechanism enables the instrument to automatically move the microplate and pipette, thereby reducing the steps and time of manual operation and improving the detection efficiency. Automated operation also helps to reduce human errors and improve the accuracy of the detection results. In addition, the components inside the instrument are reasonably arranged according to the order of detection, making the moving path of the moving mechanism more concise and reducing the problem of repeated movement of the moving mechanism;

[0031] 2. By symmetrically setting two conical protrusions on the side where the two clamping jaws are close to each other, when clamping the microplate, the four conical protrusions abut against the side wall of the microplate. The design of the conical protrusions increases the friction force when the clamping jaws contact the microplate, making the clamping of the microplate more stable. At the same time, the symmetrical setting of the conical protrusions ensures the uniform distribution of the clamping force, avoiding damage to the object or unstable clamping due to uneven clamping force;

[0032] 3. By arranging an anti-volatile reagent kit in the outer box and using the anti-volatile reagent kit to contain easily volatile detection reagents, the reagents can be effectively sealed to prevent them from volatilizing into the air, thereby ensuring the purity and stability of the reagents and improving the accuracy of the detection. In addition, by opening a groove on the side wall of the box cover and arranging a toggle block matching the groove on the clamp, when the box cover needs to be opened, the clamp can be inserted into the groove through the toggle block, and then the driving component drives the clamp to move, thereby driving the box cover to rotate and open, thereby improving the degree of automation of the detection device and making the experimental process smoother and more efficient. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0034] Figure 1 is a schematic diagram of the overall structure of an embodiment of the present invention;

[0035] Figure 2 This is a schematic diagram of the structure inside the outer box in an embodiment of the present invention;

[0036] Figure 3 is a schematic structural diagram of a liquid transfer component in an embodiment of the present invention;

[0037] Figure 4 is a schematic structural diagram of a pipette in an embodiment of the present invention;

[0038] Figure 5 is a schematic structural diagram of a clamping assembly part in an embodiment of the present invention;

[0039] Figure 6 is a schematic structural diagram of a clamping jaw portion in an embodiment of the present invention;

[0040] Figure 7 2 is a schematic diagram of the structure of the anti-volatile reagent kit according to an embodiment of the present invention;

[0041] Figure 8 is a schematic structural diagram of a pipette support portion in an embodiment of the present invention;

[0042] Fig. 9 is a schematic structural diagram of the oscillation mechanism part in an embodiment of the present invention;

[0043] Fig.10 It is a flow chart of the automatic detection method of the fully automatic drug detection instrument in the embodiment of the present invention.

[0044] 1. The oscillating mechanism is shown in FIG. 1 . The oscillating mechanism is shown in FIG. 1 . The oscillating mechanism is shown in FIG. 1 . The oscillating mechanism is shown in FIG. 1 . The oscillating mechanism is shown in FIG. 1 . The oscillating mechanism is shown in FIG. 1 . DETAILED DESCRIPTION

[0045] In order to more clearly explain the overall concept of the present invention, the following Figure 1-Figure 10 The present invention is described in further detail.

[0046] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the protection scope of the present invention is not limited by the specific embodiments disclosed below. It should be noted that the embodiments of the present invention and the features in each embodiment may be combined with each other without conflict.

[0047] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or a communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0048] The embodiment of the present invention discloses a fully automatic drug detection instrument. Figure 1 and Figure 2A fully automatic drug detection instrument includes an outer box body, which is a hollow rectangular parallelepiped structure, and glass windows are arranged on the four side walls of the outer box body. An oscillation mechanism 100, a heating mechanism 200, a reagent tank 300, an optical detection mechanism 400, a moving mechanism 500, a pipette holder 600, a microplate holder 700 and an anti-volatile reagent kit 900 are arranged inside the outer box body. The reagent tank 300 is used to store test samples and test reagents, the pipette holder 600 is used to place a pipette 521 for transferring reagents, the pipette 521 is used to extract test samples and test reagents, the microplate holder 700 is used to place a microplate for processing test samples, the moving mechanism 500 is used to move the microplate and the pipette 521, the oscillation mechanism 100 is used to oscillate the microplate, the heating mechanism 200 is used to heat the reagents in the microplate, the optical detection mechanism 400 is used to test the absorbance of the test sample, and the anti-volatile reagent kit 900 is used to store volatile test reagents.

[0049] The moving mechanism 500 is installed on the inner top of the outer box body, the reagent tank 300 is located in the middle position of the bottom of the outer box body, the pipette holder 600 and the microplate holder 700 are respectively located on both sides of the reagent tank 300, the optical detection mechanism 400 and the oscillation mechanism 100 are respectively located on the other two sides of the reagent tank 300, the heating mechanism 200 is located on the side of the oscillation mechanism 100 close to the microplate holder 700, and the anti-volatile reagent kit 900 is located on the side of the pipette holder 600 close to the optical detection mechanism 400.

[0050] By integrating the oscillation mechanism 100, the heating mechanism 200 and the optical detection mechanism 400 in the outer box, the whole process from biological preparation sample processing to result detection can be completed on one device. The design of the moving mechanism 500 enables the instrument to automatically move the microplate and the pipette 521, thereby reducing the steps and time of manual operation and improving the detection efficiency. Automated operation also helps to reduce human errors and improve the accuracy of the detection results. In addition, the components inside the instrument are reasonably arranged according to the order of detection, so that the moving path of the moving mechanism 500 is simpler and the problem of repeated movement of the moving mechanism 500 is reduced.

[0051] Reference Figure 1 and Figure 2A collection box 800 is provided between the pipette holder 600 and the oscillating mechanism 100, and the collection box 800 is used to collect the used pipettes 521. By providing the collection box 800 for recovering the pipettes 521 between the pipette holder 600 and the oscillating mechanism 100, after adding the detection reagent or the detection sample to the microplate on the oscillating mechanism 100 by using the pipette 521, the used pipette 521 is moved to the top of the collection box 800 under the drive of the moving mechanism 500, the moving mechanism 500 is separated from the pipette 521 so that it falls into the collection box 800, and the moving mechanism 500 is separated from the pipette 521 and then moves to the top of the pipette holder 600 to assemble the unused pipette 521, thereby further optimizing the moving path of the moving mechanism 500 and improving the detection efficiency.

[0052] Reference Figure 3 , Figure 4 and Figure 5 The moving mechanism 500 includes a mounting seat 510, a liquid transfer assembly 520, a clamping assembly 530, a driving assembly, a first lifting assembly, and a second lifting assembly. The driving assembly is fixedly connected to the outer box body, and the mounting seat 510 is fixedly connected to the driving assembly. In this embodiment, the driving assembly is two sets of conventional screw driving mechanisms, which are used to drive the mounting seat 510 to move in a plane parallel to the bottom surface of the outer box body.

[0053] The pipetting assembly 520 is connected to the mounting seat 510 via a first lifting assembly, which drives the pipetting assembly 520 to move in a direction perpendicular to the floor of the outer box, and the pipetting assembly 520 is detachably connected to the pipette 521. The clamping assembly 530 is connected to the mounting seat 510 via a second lifting member, which drives the clamping assembly 530 to move in a direction perpendicular to the floor of the outer box, and the clamping assembly 530 is used to clamp the microplate.

[0054] Reference Figure 3 and Figure 4 The pipetting assembly 520 includes a pipette 521 and a pipette gun 522. The pipette gun 522 is fixedly connected to the first lifting assembly. The pipette gun 522 includes a plurality of connecting nozzles 523 for connecting with the pipette 521. The connecting nozzles 523 are hollow tube structures. Two sealing rings 524 are arranged on the outer wall of the connecting nozzles 523. The two sealing rings 524 are arranged along the axial direction of the connecting nozzles 523. The connecting nozzles 523 and the pipette orifices of the pipette 521 are interference fit.

[0055] By arranging two sealing rings 524 on the outer wall of the connecting nozzle 523, the sealing effect of the connection between the connecting nozzle 523 and the pipette 521 is improved, and at the same time, the connection between the connecting nozzle 523 and the pipette 521 is made more stable, so that the liquid transmission during the pipetting process is more stable, and the error caused by liquid leakage or loose connection is reduced.

[0056] Reference Figure 3 The liquid transfer gun 522 is provided with a separation plate 525, which is provided with a plurality of through holes, which are arranged one by one with a plurality of connecting nozzles 523, and the connecting nozzles 523 are inserted into the corresponding through holes. The separation plate 525 is connected to the liquid transfer gun 522 through an electric push cylinder, and the electric push cylinder drives the separation plate 525 to slide along the axial direction of the connecting nozzle 523.

[0057] When the pipette 521 needs to be separated from the pipette gun 522 after use, the electric push cylinder is used to drive the separation plate 525 to move towards the direction close to the pipette 521, so that the separation plate 525 abuts against the end of the pipette 521, and the electric push cylinder continues to push the separation plate 525 to move. The pipette 521 is separated from the connecting nozzle 523 of the pipette gun 522 under the thrust of the separation plate 525, thereby improving the convenience of separating multiple pipettes 521 from the connecting nozzle 523 of the pipette gun 522 at the same time, thereby improving the detection efficiency.

[0058] Reference Figure 5 and Figure 6 The clamping assembly 530 includes a driving member and two clamping jaws 531. The driving member is connected to the second lifting assembly. The driving member drives the two clamping jaws 531 to move toward or away from each other. Two conical protrusions 532 are arranged on the side where the two clamping jaws 531 are close to each other. The two conical protrusions 532 are symmetrically arranged. In this embodiment, the driving member is a conventional bidirectional screw drive structure.

[0059] When clamping the microplate, the four conical protrusions 532 abut against the side wall of the microplate. The design of the conical protrusions 532 increases the friction between the clamping jaws 531 and the microplate, making the clamping of the microplate more stable. At the same time, the symmetrical arrangement of the conical protrusions 532 ensures uniform distribution of the clamping force, avoiding damage to the object or unstable clamping due to uneven clamping force.

[0060] Reference Figure 7 The anti-volatile reagent kit 900 includes a box body 910 and a box cover 920. The box body 910 is used to accommodate volatile detection reagents. The box cover 920 is hinged to the box body 910. The box cover 920 is used to close the box opening of the box body 910. A groove 921 is provided on the side wall of the box cover 920. The length direction of the groove 921 is perpendicular to the rotation axis of the box cover 920. A toggle block 533 for inserting into the groove 921 is provided on the side wall of the clamping jaw 531 away from the conical protrusion 532.

[0061] By providing an anti-volatile reagent kit 900 in the outer box body and using the anti-volatile reagent kit 900 to accommodate easily volatile detection reagents, the reagents can be effectively sealed to prevent them from volatilizing into the air, thereby ensuring the purity and stability of the reagents and improving the accuracy of the detection. In addition, by providing a groove 921 on the side wall of the box cover 920 and providing a toggle block 533 matching the groove 921 on the clamp 531, when the box cover 920 needs to be opened, the clamp 531 can be inserted into the groove 921 through the toggle block 533, and then the driving component drives the clamp 531 to move, thereby driving the box cover 920 to rotate and open, thereby improving the degree of automation of the detection device and making the experimental process smoother and more efficient.

[0062] Reference Figure 5 , the toggle block 533 is a cylindrical structure, and one end of the toggle block 533 is fixedly connected to the clamp 531. The pipette holder 600 limits the opening angle of the box cover 920 to be less than 180 degrees. By setting the toggle block 533 as a cylindrical structure, the cooperation between the toggle block 533 and the groove 921 is smoother, and the accuracy and stability of opening the box cover 920 are improved. When the detection reagent in the anti-volatile reagent kit 900 is taken out, the toggle block 533 on the clamp 531 is used to push the box cover 920 to rotate on the side of the box cover 920 away from the box body 910, so that the box cover 920 is buckled on the box body 910.

[0063] Reference Figure 8 The pipette holder 600 includes a holder body 610 and a pipette placement plate 620. The pipette placement plate 620 is provided with a plurality of placement holes 621 for inserting pipettes 521. The pipette placement plate 620 is detachably connected to the holder body 610. The anti-volatile reagent kit 900 is located on one side of the pipette holder 600. The pipette holder 600 limits the opening angle of the box cover 920 to be less than 180 degrees.

[0064] Reference Fig. 9 The oscillation mechanism 100 includes a tray 110 for clamping the microplate, and spring discs 120 are fixedly connected to any three inner walls of the tray 110. By clamping the microplate with the tray 110, the microplate can be firmly fixed on the oscillation mechanism 100, avoiding experimental errors caused by looseness during the oscillation process. The spring disc 120 has excellent elasticity and recovery, and can generate stable vibration waves during the oscillation process, so that the liquid or sample in the microplate can be evenly mixed. In addition, the spring disc 120 is fixedly connected to any three inner walls of the tray 110, so that the positioning of the microplate is more accurate, and the stability of the clamping assembly 530 clamping the microplate is improved.

[0065] This embodiment also discloses an automatic detection method of a fully automatic drug detection instrument, which mainly includes the following steps.

[0066] In step 101, the target position information is configured for the pipette so that the pipette moves automatically according to the target position information. In this way, the pipette can obtain the specific target position to be moved to, and then it can move automatically to ensure that it reaches the accurate position. The pipette can be a pipette gun 522, and the target position can refer to the position of the gun tip, the position of each different reagent, the position of the oscillator, etc.

[0067] In step 102, the real-time position information of the pipette is obtained. In step 103, when the pipette is at the operating point, a liquid level detection instruction is sent to the liquid level detection sensor, and the current liquid level information is obtained. When the pipette reaches the liquid taking position, it means that the pipette is about to take liquid, so the liquid level detection is first performed to obtain the current liquid level information.

[0068] In step 104, based on the current liquid level information, if the current liquid level information is inconsistent with the preset empty liquid level information, the vertical moving distance is calculated based on the current liquid level information. This can avoid empty liquid collection when there is no liquid level, resulting in the false impression that liquid has been collected, and can improve the accuracy of detection.

[0069] In step 1041, the first parameter of the corresponding container is obtained by looking up the table based on the current operation point position. The containers for holding solutions at different operation points are different, so the heights are different, which facilitates determining the height of the pipette and the liquid level and subsequently determining the end point position of the movement.

[0070] In step 1042, when taking liquid, based on the current amount of liquid taken, when the current amount of liquid taken is greater than the first threshold value, the current liquid level information is corrected. Because the amount of liquid taken each time is about 5mL, if the amount of liquid taken is large, the liquid level changes greatly, and the liquid level detection sensor has a certain error. The pipette takes liquid through the pipette gun 522 head, and the pipette is connected to the pipette gun 522 head. The height of the pipette gun 522 head is about 5cm. When taking liquid or discharging liquid, the pipette gun 522 head needs to extend below the liquid level, so that the pipette gun 522 head will be stained with part of the liquid, which may affect the accuracy of the detection, so the bottom of the pipette gun 522 head is controlled to extend 3-4mm below the liquid level. In the process of taking liquid, the liquid level will also have a downward fluctuation. Therefore, in order to accurately control the pipette gun 522 head to extend below the liquid level, it is necessary to set parameters according to the change of the amount of liquid taken and the current amount of liquid taken, and correct the current liquid level information.

[0071] In step 1043, based on the first parameter and the corrected liquid level information, the vertical moving distance is calculated and sent to the pipette to control the depth of the pipette under the liquid surface. In this way, the depth of the pipette can be accurately controlled. Knowing the height of the pipette gun 522 head from the top of the container, and then combining the height of the liquid surface, the distance that the pipette gun 522 moves downward can be calculated.

[0072] Therefore, the processes of taking liquid, preparing liquid and testing are performed in sequence according to the position reached by the pipette to accurately complete the testing process, and the automatic testing process can be accurately completed when the testing process requires a variety of different reagents and multiple tests.

[0073] In some embodiments, based on the current liquid withdrawal amount, when the current liquid withdrawal amount is greater than the first threshold, the current liquid level information is corrected, including: using formula (1) to obtain the corrected liquid level information,

[0074] H = h - ((L × σ) / 5) (1)

[0075] Wherein, L represents the current liquid volume, h represents the currently detected liquid level, and σ represents the coefficient, and its value range is 0.8-1.

[0076] The first threshold value can be 3mL, etc. The value of σ can be selected according to the type of solution. For solutions with higher viscosity, a larger value is selected. It can also be determined according to the current amount of liquid taken. The larger the current amount of liquid taken, the larger σ is selected. For example, 10cm is detected, 3mL was taken last time, and σ is 0.8, then H=9.62cm. In this way, the height of the descent of the pipette 522 head can be controlled, the accuracy of the liquid level height can be improved, and it can also ensure that the depth of the pipette 522 head inserted into the liquid surface will not fluctuate greatly, and the liquid can be taken, and the amount taken will not be less than the predetermined amount due to the fluctuation of the liquid level.

[0077] In some embodiments, the pipette is configured with target position information so that the pipette moves automatically according to the target position information, including: obtaining the position information of each target point; using the pipette as the center point of the three-dimensional coordinate system, and based on the position information of each target point, obtaining the target coordinate point information of each target point in the three-dimensional coordinate system; sending the target coordinate point information of the liquid preparation point and each liquid collection point to the pipette, so that the pipette moves according to the target coordinate point information.

[0078] The position information of each target point can be manually input information and stored in advance. Then, the initial position of the pipette is used as the center point of the three-dimensional coordinate system to obtain the relative distance of each target point relative to the initial position of the pipette, so that the target coordinate point information of each target point in the three-dimensional coordinate system, the information in the X direction, the information in the Y direction, and the information in the Z direction can be obtained, and then the pipette can be moved conveniently. In some embodiments, the automatic detection method also includes: obtaining the X-axis coordinate information, the Y-axis coordinate information and the Z-axis coordinate information of the target point according to the target coordinate point information; and moving in sequence according to the X-axis coordinate information, the Y-axis coordinate information and the Z-axis coordinate information. For example, if the target coordinates are (10000, 3000, 2000), the X-axis is first moved to 10000, then the Y-axis is moved to 3000, and then the Z-axis is moved to 2000. Since there are many types of reagents and the dispensing points are in different positions, the precise positioning of the target position information facilitates the pipette to accurately complete the detection process. The pipette can select the middle position between the top of the pipette and the top of the container containing the solution as the end position after the move.

[0079] In some embodiments, the automatic detection method also includes: obtaining the real-time position information of the pipette, and converting it into the real-time coordinate information in the three-dimensional coordinate system, and sending an alarm message when the real-time coordinate information is not within the range of the target coordinate point information. The position information of the pipette can be obtained by a position sensor, for example, a position sensor is set on the pipette. The real-time position information of the pipette can be obtained at intervals of a first time interval, such as 5s. If the real-time coordinate information is within the range of the target coordinate point information, it means that the pipette is in the normal pipetting process, and no alarm is required, and the movement can be continued. If the real-time coordinate information is not within the range of the target coordinate point information, it means that the normal path of the pipette deviates, and an alarm message is sent.

[0080] In some embodiments, when the real-time coordinate information is within the range of the target coordinate point information, an alarm message is issued, including:

[0081] The coordinate values ​​of the X, Y and Z axes in the real-time coordinate information are compared with the coordinate values ​​of the X, Y and Z axes in the target coordinate point information. When the coordinate values ​​of the X, Y and Z axes in the real-time coordinate information are greater than the coordinate values ​​of the X, Y and Z axes in the target coordinate point information, an alarm message is issued. For example, if the target coordinates are (10000, 3000, 2000) and the real-time position information is (10000, 2000, 0), it means that the real-time coordinate information is within the range of the target coordinate point information.

[0082] In some embodiments, the pipette returns to the initial position after moving to a target point, and then moves to the next target point, so as to avoid position movement errors caused by problems such as recognition of coordinate information when moving directly from one target point to the next target point.

[0083] In some embodiments, when the current liquid level information is inconsistent with the preset empty liquid level information, a liquid taking instruction is issued to the pipette, including: the current liquid level information and the empty liquid level information are respectively the current voltage value and the empty liquid level voltage value, and when the current voltage value and the empty liquid level voltage value are inconsistent, a liquid taking instruction is issued to the pipette. A voltage converter is provided inside the liquid level detection sensor, and after the liquid level detection sensor detects the liquid level, the voltage converter will output a corresponding voltage value, so as to automatically determine the liquid level condition.

[0084] In some embodiments, the automatic detection method further includes: when the current liquid level information is consistent with the preset empty liquid level information, issuing a reminder message for adding liquid. If the liquid level is empty, no liquid is taken, and a reminder is issued so that the staff can replenish the liquid in time.

[0085] The operation points include liquid collection points, liquid preparation points and / or detection points, etc.

[0086] When the pipette is at the liquid dispensing point, a liquid dispensing instruction is sent to the liquid dispensing device. After the liquid dispensing operation is completed, a liquid removal instruction is sent to the pipette. The liquid dispensing device may include an oscillator or a heater, etc. When the pipette is at the liquid dispensing point, the oscillator oscillates according to the liquid dispensing instruction to fully mix the liquid, or the heater heats the liquid according to the liquid dispensing instruction. When the pipette is at the detection point, a detection instruction is sent to the detection equipment so that the detection equipment performs detection and obtains the detection result. The detection equipment can be an optical analysis system, which can obtain an optical analysis result.

[0087] According to an embodiment of the present application, an automatic detection system of a fully automatic drug detection instrument is also provided. The automatic detection system includes a pipette, a control module, and a liquid level detection sensor. The control module is configured to: configure target position information for the pipette so that the pipette moves automatically according to the target position information; obtain real-time position information of the pipette; when the pipette is at the operating point position, send a liquid level detection instruction to the liquid level detection sensor and obtain current liquid level information; based on the current liquid level information, when the current liquid level information is inconsistent with the preset empty liquid level information, calculate the vertical movement distance based on the current liquid level information, including: obtaining the first parameter of the corresponding container by looking up the table based on the current sampling point position; when taking liquid, based on the current liquid taking amount, when the current liquid taking amount is greater than the first threshold, correct the current liquid level information; based on the first parameter and the corrected liquid level information, calculate the vertical movement distance, and send the movement distance to the pipette to control the depth of the pipette extending below the liquid surface.

[0088] Different reagents, gun tips, and containers (such as reagent tank 300 and anti-volatile reagent kit 900) are placed in different positions, and the pipette is located at the top. Through the execution steps of the control module, the automatic detection process can be better completed accurately based on the fully automatic drug detection instrument. The control module is connected to the pipette and the liquid level detection sensor to transmit signals.

[0089] In some embodiments, the automatic detection system further includes a display module to display the detection results and the progress of the detection, etc. The display module may also be configured with a touch screen to facilitate the operator to perform corresponding operations. The relevant information of the control module may be output to the control module for display.

[0090] In some embodiments, the automatic detection system further includes a position sensor, for example, a position sensor is provided on the pipette, and the position information of the pipette is obtained through the position sensor.

[0091] In some embodiments, the pipette is configured with target position information so that the pipette moves automatically according to the target position information, including: obtaining the position information of each target point; using the pipette as the center point of the three-dimensional coordinate system, and based on the position information of each target point, obtaining the target coordinate point information of each target point in the three-dimensional coordinate system; sending the target coordinate point information of each target point to the pipette, so that the pipette moves according to the target coordinate point information.

[0092] In some embodiments, the control module is further configured to: obtain the real-time position information of the pipette, and convert it into real-time coordinate information in a three-dimensional coordinate system, and issue an alarm message when the real-time coordinate information is not within the range of the target coordinate point information.

[0093] According to an embodiment of the present application, a non-transitory computer-readable medium is also provided, on which instructions are stored. When executed by a processor, the instructions execute the steps of the automatic detection method of the fully automatic drug detection instrument of any embodiment of the present application.

[0094] The various embodiments in this specification are described in a progressive manner, and the same or similar parts between the various embodiments can be referenced to each other, and each embodiment focuses on the differences from other embodiments.

[0095] The above are all preferred embodiments of the present invention, and are not intended to limit the protection scope of the present invention. Therefore, any equivalent changes made according to the structure, shape, and principle of the present invention should be included in the protection scope of the present invention. Anything not described in the present invention can be achieved by adopting or drawing on existing technologies.

Claims

1. A fully automatic drug detection instrument, characterized in that: The invention comprises an outer box body, wherein an oscillating mechanism (100), a heating mechanism (200), a reagent tank (300), an optical detection mechanism (400), a moving mechanism (500), a pipette support (600) and a microplate support (700) are arranged inside the outer box body, wherein the reagent tank (300) is used to store detection samples and detection reagents, the pipette support (600) is used to place a pipette (521) for transferring reagents, the pipette (521) is used to extract detection samples and detection reagents, the microplate support (700) is used to place a microplate for processing detection samples, the moving mechanism (500) is used to move the microplate and the pipette (521), and the oscillating mechanism ( The outer box body (100) is used to oscillate the microplate, the heating mechanism (200) is used to heat the reagent in the microplate, the optical detection mechanism (400) is used to test the absorbance of the test sample, the moving mechanism (500) is connected to the inner top of the outer box body, the reagent tank (300) is located in the middle of the bottom of the outer box body, the pipette support (600) and the microplate support (700) are respectively located on two sides of the reagent tank (300), the optical detection mechanism (400) and the oscillation mechanism (100) are respectively located on the other two sides of the reagent tank (300), and the heating mechanism (200) is located on a side of the oscillation mechanism (100) close to the microplate support (700); The moving mechanism (500) comprises a mounting seat (510), a pipetting assembly (520), a clamping assembly (530), a driving assembly, a first lifting assembly and a second lifting assembly, wherein the driving assembly is connected to the outer box body, the mounting seat (510) is connected to the driving assembly, the driving assembly drives the mounting seat (510) to move in a plane parallel to the bottom surface of the outer box body, the pipetting assembly (520) is connected to the mounting seat (510) via the first lifting assembly, the first lifting assembly drives the pipetting assembly (520) to move in a direction perpendicular to the bottom surface of the outer box body, the pipetting assembly (520) is detachably connected to the pipette (521), the clamping assembly (530) is connected to the mounting seat (510) via the second lifting member, the second lifting assembly drives the clamping assembly (530) to move in a direction perpendicular to the bottom surface of the outer box body, and the clamping assembly (530) is used to clamp the microplate; The pipetting assembly (520) comprises a pipette (521) and a pipette gun (522); the pipette gun (522) comprises a plurality of connecting nozzles (523) for connecting to the pipette (521); the connecting nozzles (523) are hollow tube structures; two sealing rings (524) are arranged on the outer wall of the connecting nozzle (523); the two sealing rings (524) are arranged along the axial direction of the connecting nozzle (523); the connecting nozzle (523) is interference fit with the tube mouth of the pipette (521).

2. A fully automatic drug detection instrument according to claim 1, characterized in that: A collection box (800) is provided between the pipette support (600) and the oscillating mechanism (100), and the collection box (800) is used to collect used pipettes (521).

3. A fully automatic drug detection instrument according to claim 1, characterized in that: The pipette gun (522) is provided with a separation plate (525), and a plurality of through holes are opened on the separation plate (525). The plurality of through holes are arranged in a one-to-one correspondence with a plurality of connecting nozzles (523), and the connecting nozzles (523) are inserted into the corresponding through holes. The separation plate (525) is connected to the pipette gun (522) via an electric push cylinder, and the electric push cylinder drives the separation plate (525) to slide along the axial direction of the connecting nozzle (523).

4. A fully automatic drug detection instrument according to claim 3, characterized in that: The clamping assembly (530) comprises a driving member and two clamping jaws (531), wherein the driving member is connected to the second lifting assembly, and the driving member drives the two clamping jaws (531) to move in a direction of approaching or moving away from each other, and two conical protrusions (532) are arranged on the side where the two clamping jaws (531) approach each other, and the two conical protrusions (532) are symmetrically arranged.

5. A fully automatic drug detection instrument according to claim 4, characterized in that: An anti-volatile reagent kit (900) is arranged in the outer box body, and the anti-volatile reagent kit (900) is used to accommodate a detection reagent that is easily volatile. The anti-volatile reagent kit (900) comprises a box body (910) and a box cover (920), and the box body (910) is used to accommodate a detection reagent that is easily volatile. The box cover (920) is hinged to the box body (910), and the box cover (920) is used to seal the box opening of the box body (910). A groove (921) is provided on the side wall of the box cover (920), and the length direction of the groove (921) is perpendicular to the rotation axis of the box cover (920). A toggle block (533) for inserting into the groove (921) is provided on the side wall of the clamping jaw (531) away from the conical protrusion (532).

6. A fully automatic drug detection instrument according to claim 5, characterized in that: The pipette support (600) comprises a support body (610) and a pipette placement plate (620); the pipette placement plate (620) is provided with a plurality of placement holes (621) for inserting pipettes (521); the pipette placement plate (620) is detachably connected to the support body (610); the anti-volatile reagent kit (900) is located on one side of the pipette support (600); and the pipette support (600) limits the opening angle of the box cover (920) to less than 180 degrees.

7. A fully automatic drug detection instrument according to claim 1, characterized in that: The oscillating mechanism (100) comprises a support tray (110) for clamping a microplate, and spring discs (120) are fixedly connected to any three inner walls of the support tray (110).

8. An automatic detection method for a fully automatic drug detection instrument, characterized in that: The automatic detection method is applied to the fully automatic drug detection instrument described in any one of claims 1 to 7, and the automatic detection method comprises: configuring target position information for the pipetting component so that the pipetting component automatically moves according to the target position information; Obtain real-time position information of pipetting components; When the liquid transfer assembly is at the operating point, a liquid level detection instruction is sent to the liquid level detection sensor, and current liquid level information is obtained; Based on the current liquid level information, when the current liquid level information is inconsistent with the preset empty liquid level information, the vertical movement distance is calculated based on the current liquid level information, including: obtaining a first parameter of the corresponding container by looking up a table based on the current operation point position; when taking liquid, based on the current liquid taking amount, when the current liquid taking amount is greater than a first threshold, correcting the current liquid level information; based on the first parameter and the corrected liquid level information, calculating the vertical movement distance, and sending the movement distance to the pipetting component to control the depth of the pipetting component extending below the liquid surface.

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