A device for inducing rapid propagation of tissue cultured woody flowers

Abstract

The invention discloses an induction device for rapid propagation of tissue culture woody flowers, and relates to the technical field of plant tissue culture. The device comprises a culture cabinet and a tissue culture bottle. A bottle rotating mechanism, a humidifying mechanism, a bottle pressing mechanism and a vibrating mechanism are arranged in the culture cabinet. A nutrient solution supplementing mechanism is arranged in the tissue culture bottle. A culture chamber is arranged in the culture cabinet. An illuminating lamp is arranged in the culture chamber. The tissue culture bottle is detachably installed in the culture chamber. The tissue culture bottle comprises a bottle body and a bottle cap, which are threadedly connected to the bottle body. The bottle rotating mechanism comprises a turntable, a transmission shaft, a driving shaft and a driving motor. The driving shaft is arranged on the output shaft of the driving motor, and the driving shaft drives the turntable through the transmission shaft. The tissue culture bottle is arranged on the upper end of the turntable. The device has the advantages of uniform illumination and stable nutrient supply, and solves the problems of uneven illumination of the culture box and unstable nutrient supply affecting plant growth in traditional tissue culture technology.

Description

Technical Field

[0001] The invention relates to the technical field of plant tissue culture, in particular to an induction device for rapid propagation of tissue culture woody flowers. Background Art

[0002] Plant tissue culture (abbreviated as tissue culture) is a technology that uses a part of a plant (such as stem tip, leaf, root, etc.) to culture in an artificially controlled environment under sterile conditions to obtain a complete plant. Tissue culture technology is widely used in the fields of rapid plant propagation, germplasm preservation, genetic improvement, etc., and has the advantages of fast propagation speed, high propagation coefficient, and no seasonal restrictions. However, traditional tissue culture technology has some limitations.

[0003] In the traditional tissue culture process, plant tissues are usually placed statically in tissue culture bottles, resulting in uneven lighting. Plants bend excessively due to phototropism, affecting the growth quality and consistency of flowers. Most existing tissue culture bottles cannot replenish nutrient solution, which can easily cause excessive consumption or insufficient supply of nutrient solution in the later stage of plant growth, affecting the healthy growth of plants. Therefore, an induction device for rapid propagation of tissue cultured woody flowers is needed to solve the above problems. Summary of the invention

[0004] The purpose of the present invention is to provide an induction device for rapid propagation of tissue culture woody flowers, which has the advantages of uniform illumination and stable nutrient supply, and solves the problem of uneven illumination and unstable nutrient supply in the culture box affecting plant growth in traditional tissue culture technology.

[0005] To achieve the above object, the present invention provides the following technical solution: an induction device for rapid propagation of tissue culture woody flowers, comprising a culture cabinet and a tissue culture bottle, wherein the culture cabinet is provided with a bottle rotating mechanism, a humidifying mechanism, a bottle pressing mechanism and a vibrating mechanism, and the tissue culture bottle is provided with a nutrient solution supplementing mechanism;

[0006] The culture cabinet is provided with a culture chamber, the culture chamber is provided with an illuminating lamp, the tissue culture bottle is detachably installed in the culture chamber, the tissue culture bottle comprises a bottle body and a bottle cap, and the bottle cap is threadedly connected to the bottle body;

[0007] The rotating bottle mechanism comprises a rotating disk, a transmission shaft, a driving shaft and a driving motor, wherein the driving shaft is mounted on the output shaft of the driving motor, the driving shaft drives the rotating disk through the transmission shaft, and the tissue culture bottle is mounted on the upper end of the rotating disk;

[0008] The nutrient solution supplement mechanism comprises a nutrient solution tube, an elastic diaphragm, a liquid supplement tube and an air intake tube, and the nutrient solution supplement mechanism is installed on the bottle cap;

[0009] The humidification mechanism comprises a water tank, a submersible pump, an atomizing nozzle and a water supply pipe, and the humidification mechanism is installed at the bottom of the inner end surface of the culture cabinet;

[0010] The bottle pressing mechanism comprises a fixing frame, a pressing rod and a sleeve, and the bottle pressing mechanism is fixedly installed on the top of the culture chamber;

[0011] The rapping mechanism comprises a driving wheel, a knocking rod, a guide frame and a spring, and the rapping mechanism is installed on the top of the driving shaft.

[0012] As a preferred induction device for rapid propagation of tissue cultured woody flowers of the present invention, the driving shaft is installed on the output shaft of the driving motor through a reducer, a first rotating shaft is provided at the bottom of the turntable, a worm gear is fixedly mounted on the first rotating shaft, a worm matching the worm gear is provided on the transmission shaft, and the transmission shaft and the driving shaft are transmitted through the meshing of toothed bevels.

[0013] As a preferred induction device for rapid propagation of tissue cultured woody flowers of the present invention, the upper end surface of the turntable is provided with a positioning groove corresponding to the shape of the bottom of the bottle body, a transmission column is provided at the center of the positioning groove, and the lower end surface of the bottom of the bottle body is provided with a transmission groove that slides with the transmission column, and the transmission column is a regular polygonal prism structure.

[0014] As a preferred induction device for rapid propagation of tissue cultured woody flowers of the present invention, a first screw hole is provided on the bottle cap, the elastic diaphragm is fixedly mounted on the upper end surface of the first screw hole, the top outer end surface of the nutrient solution tube is provided with an external thread, the nutrient solution tube is inserted into the first screw hole and is threadedly connected to the bottle cap, the liquid replenishment tube and the air inlet pipe are installed on the side wall of the nutrient solution tube and are through-connected to the nutrient solution tube, one-way valves are provided in the liquid replenishment tube and the air inlet pipe, a breathable membrane is provided in the center of the bottle cap, and the pipe mouth of the liquid replenishment tube is flush with the bottom inner end surface of the liquid replenishment tube.

[0015] As a preferred induction device for rapid propagation of tissue cultured woody flowers of the present invention, the elastic diaphragm is a hemispherical surface structure, and the elastic diaphragm includes a rubber membrane and an elastic reed.

[0016] As a preferred induction device for rapid propagation of tissue cultured woody flowers of the present invention, the fixed frame is fixedly installed on the top lower end surface of the culture chamber, a slide groove is provided at the bottom of the fixed frame, and sliding rods that slide with the slide groove are provided at the left and right ends of the pressure rod, and the sleeve is sleeved on the pressure rod and rotatably connected to it.

[0017] As a preferred induction device for rapid propagation of tissue cultured woody flowers of the present invention, the lower end face of the fixing frame is provided with an axial hole, a rotatably connected adjusting knob is provided in the axial hole, a second screw hole is provided on the lower end face of the adjusting knob, and a screw rod cooperating with the second screw hole is provided on the upper end face of the pressure rod.

[0018] As a preferred induction device for rapid propagation of tissue cultured woody flowers of the present invention, the driving wheel is fixedly mounted on the top of the driving shaft, the guide frame is fixedly mounted on the inner end surface of the culture cabinet, the guide frame is provided with a guide groove, the knocking rod and the spring are installed in the guide groove and are slidably connected to the guide frame, the side surface of the driving wheel is provided with a first convex tooth, and the side surface of the knocking rod is provided with a second convex tooth matching the first convex tooth.

[0019] As a preferred induction device for rapid propagation of tissue cultured woody flowers of the present invention, the top and bottom of the culture chamber are inclined structures, there are two lighting lamps in each culture chamber, the two lighting lamps are respectively located on the front and rear sides of the bottle pressing mechanism, and a drainage hole is provided on the front end face of the culture chamber.

[0020] As a preferred induction device for rapid propagation of tissue cultured woody flowers of the present invention, the water tank is fixedly mounted on the upper end surface of the bottom of the culture cabinet, the upper end surface of the water tank is provided with a reflux port corresponding to the position of the drainage hole, the submersible pump is fixedly mounted in the water tank, the atomizing nozzle is fixedly mounted on the side wall of the culture room, and the submersible pump is connected to the atomizing nozzle through a water supply pipe.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. The present invention provides a rotating bottle mechanism so that the plant tissue in the tissue culture bottle can receive light evenly, reducing blind spots in light, solving the problem of excessive bending of plants due to phototropism in traditional static culture, and avoiding uneven growth of plants due to insufficient or excessive local light. The rotating bottle mechanism rotates slowly to ensure that the plant tissue can obtain sufficient light in all directions, thereby promoting photosynthesis of the plant and improving the utilization rate of light energy. Therefore, the growth quality and consistency of the plant are significantly improved, which is conducive to cultivating plants with good morphology and strong growth.

[0023] 2. The cooperation between the nutrient solution replenishing mechanism and the bottle pressing mechanism of the present invention realizes the intermittent replenishment of the nutrient solution in the tissue culture bottle. This mechanism can provide necessary nutrients in a timely and appropriate amount according to the actual growth needs of the plants, avoiding excessive consumption and waste of the nutrient solution. Through the supply of nutrient solution, it is ensured that the plants can obtain sufficient and balanced nutrition supply throughout the growth cycle, especially in the rapid growth period and key development stage, which can effectively support the healthy growth of plants, improve the growth rate and survival rate of plants, and reduce growth disorders caused by malnutrition.

[0024] 3. The present invention can not only effectively control the humidity in the culture room and ensure that it is maintained at an optimal level suitable for plant growth through the cooperation of the humidifying mechanism and the vibrating mechanism, but also reduce the accumulation of water droplets on the inner wall through the vibrating mechanism to prevent the water droplets from causing local over-humidity in the culture box. The vibrating mechanism vibrates at a fixed time to shake the water droplets on the inner wall off, causing them to slide down the inclined surface and flow back to the humidifying mechanism, thereby realizing the recycling of water resources. This design not only optimizes the humidity management in the culture room, ensures the stability and comfort of the plant growth environment, but also greatly saves water resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0026] Figure 2 It is a front view of the present invention;

[0027] Figure 3 For the present invention Figure 2 Middle AA section;

[0028] Figure 4 For the present invention Figure 3 Middle BB section;

[0029] Figure 5 For the present invention Figure 1 Enlarged view of point F in the middle;

[0030] Figure 6 It is a schematic diagram of the cooperation state between the bottle rotating mechanism of the present invention, the tissue culture bottle and the vibrating mechanism;

[0031] Figure 7 For the present invention Figure 3 Enlarged view of point G in the middle;

[0032] Figure 8 For the present invention Figure 3 Enlarged view of point C in the middle;

[0033] Fig. 9 For the present invention Figure 6 Enlarged view of point D in the middle;

[0034] Fig.10 It is a front cross-sectional view of the bottle pressing mechanism of the present invention;

[0035] Fig.11 For the present invention Fig.10 Enlarged view of point E in the middle.

[0036] In the figure: 1. culture cabinet; 101. culture room; 1011. drainage hole; 1012. lighting lamp; 102. cabinet door; 2. tissue culture bottle; 201. bottle body; 2011. transmission slot; 202. bottle cap; 2021. breathable membrane; 2022. first screw hole; 3. bottle rotating mechanism; 301. turntable; 3011. positioning slot; 3012. transmission column; 3013. first rotating shaft; 3014. worm gear; 302. transmission shaft; 3021. worm; 303. drive shaft; 304. drive motor; 4. nutrient solution replenishing mechanism; 401. nutrient solution tube; 402. elastic diaphragm; 4021. rubber membrane; 4022. elastic spring Plate; 403, liquid replenishment tube; 4031, one-way valve; 404, air inlet pipe; 5, humidification mechanism; 501, water tank; 5011, reflux port; 502, submersible pump; 503, atomizing nozzle; 504, water supply pipe; 6, bottle pressing mechanism; 601, fixing frame; 6011, slide groove; 6012, shaft hole; 602, pressure rod; 6021, slide rod; 6022, screw rod; 603, sleeve; 604, adjustment knob; 6041, second screw hole; 7, vibration mechanism; 701, driving wheel; 7011, first convex tooth; 702, knocking rod; 7021, second convex tooth; 703, guide frame; 7031, guide groove; 704, spring. DETAILED DESCRIPTION

[0037] Example 1

[0038] See also Figure 1-Figure 11 , an induction device for rapid propagation of tissue culture woody flowers, comprising a culture cabinet 1 and a tissue culture bottle 2, wherein the culture cabinet 1 is provided with a bottle rotating mechanism 3, a humidifying mechanism 5, a bottle pressing mechanism 6 and a vibrating mechanism 7, and the tissue culture bottle 2 is provided with a nutrient solution supplementing mechanism 4;

[0039] The front end surface of the culture cabinet 1 is provided with a cabinet door 102, the front end surface of the culture cabinet 1 is provided with a PLC controller, a humidity sensor is provided in the culture cabinet 1, and is used to control the speed and start and stop of the drive motor 304, as well as the start and stop of the submersible pump 502 and the lighting lamp 1012. The culture cabinet 1 is provided with a culture chamber 101, and the culture chamber 101 is provided with a lighting lamp 1012. The tissue culture bottle 2 is detachably installed in the culture chamber 101, and the tissue culture bottle 2 includes a bottle body 201 and a bottle cap 202, and the bottle cap 202 is threadedly connected to the bottle body 201;

[0040] The bottle rotating mechanism 3 includes a rotating disk 301, a transmission shaft 302, a driving shaft 303 and a driving motor 304. The driving shaft 303 is mounted on the output shaft of the driving motor 304. The driving shaft 303 drives the rotating disk 301 through the transmission shaft 302. The tissue culture bottle 2 is mounted on the upper end of the rotating disk 301.

[0041] The nutrient solution replenishing mechanism 4 includes a nutrient solution tube 401, an elastic diaphragm 402, a liquid replenishing tube 403 and an air inlet tube 404. The nutrient solution replenishing mechanism 4 is installed on the bottle cap 202;

[0042] The humidification mechanism 5 includes a water tank 501, a submersible pump 502, an atomizing nozzle 503 and a water supply pipe 504. The humidification mechanism 5 is installed at the bottom of the inner end surface of the culture cabinet 1;

[0043] The bottle pressing mechanism 6 includes a fixing frame 601, a pressing rod 602 and a sleeve 603, and the bottle pressing mechanism 6 is fixedly installed on the top of the culture chamber 101;

[0044] The rapping mechanism 7 includes a driving wheel 701 , a knocking rod 702 , a guide frame 703 and a spring 704 . The rapping mechanism 7 is installed on the top of the driving shaft 303 .

[0045] The tissue culture bottle 2 is driven to rotate by the bottle rotating mechanism 3, so that the plant tissue in the tissue culture bottle 2 is illuminated more evenly, and the nutrient solution replenishing mechanism 4 and the bottle pressing mechanism 6 are intermittently coordinated, so as to intermittently replenish the nutrient solution to the tissue culture bottle 2, so as to avoid the culture medium in the tissue culture bottle 2 from consuming nutrients too quickly, resulting in insufficient nutrition in the later stage and affecting the growth of flowers. The culture chamber 101 is humidified by the humidifying mechanism 5 to ensure that the environmental humidity in the culture cabinet 1 is at the optimal humidity for plant growth. The water droplets on the inner wall of the culture cabinet 1 are shaken off by the vibrating mechanism 7, and the water droplets are returned to the humidifying mechanism 5, thereby reducing the water droplets on the inner wall of the equipment, making the humidity in the equipment more uniform, and at the same time improving the service life of the equipment.

[0046] Furthermore, the driving shaft 303 is installed on the output shaft of the driving motor 304 through a reducer, a first rotating shaft 3013 is arranged at the bottom of the turntable 301, a worm gear 3014 is fixedly mounted on the first rotating shaft 3013, a worm 3021 cooperating with the worm gear 3014 is arranged on the transmission shaft 302, and the transmission shaft 302 and the driving shaft 303 are transmitted through the meshing of toothed bevels.

[0047] The driving motor 304 drives the driving shaft 303 to rotate slowly, and the driving shaft 303 drives the transmission shaft 302 to rotate slowly. The worm wheel 3014 and the worm 3021 cooperate to make the turntable 301 spin very slowly, so that the turntable 301 drives the tissue culture bottle 2 to rotate back, so that the plant tissue in the tissue culture bottle 2 can be evenly illuminated, reducing the blind angle of illumination, and avoiding excessive bending under the effect of phototropism, which affects the quality of flowers.

[0048] Furthermore, the upper end surface of the turntable 301 is provided with a positioning groove 3011 corresponding to the bottom shape of the bottle body 201, and a transmission column 3012 is provided at the center of the positioning groove 3011. The lower end surface of the bottom of the bottle body 201 is provided with a transmission groove 2011 that slides with the transmission column 3012, and the transmission column 3012 is a regular polygonal prism structure.

[0049] The positioning groove 3011 improves the stability of the bottle body 201 fixed by the bottle rotating mechanism 3 to prevent the bottle body 201 from shifting or tipping over during rotation. The transmission column 3012 cooperates with the transmission groove 2011 to ensure that the bottle body 201 can rotate with the turntable 301 during rotation of the turntable 301, thereby preventing the bottle body 201 from slipping and being unable to rotate effectively.

[0050] Furthermore, a first screw hole 2022 is provided on the bottle cap 202, and the elastic diaphragm 402 is fixedly installed on the upper end surface of the first screw hole 2022. The top outer end surface of the nutrient solution tube 401 is provided with an external thread. The nutrient solution tube 401 is inserted into the first screw hole 2022 and is threadedly connected to the bottle cap 202. The liquid infusion tube 403 and the air intake tube 404 are installed on the side wall of the nutrient solution tube 401 and are connected to the nutrient solution tube 401. A one-way valve 4031 is provided in the liquid infusion tube 403 and the air intake tube 404. A breathable membrane 2021 is provided in the center of the bottle cap 202, and the pipe mouth of the liquid infusion tube 403 is flush with the bottom inner end surface of the liquid infusion tube 403.

[0051] When the elastic diaphragm 402 is pressed, the air pressure in the nutrient solution tube 401 increases, thereby squeezing out the nutrient solution in the nutrient solution tube 401 and replenishing the nutrient solution in the tissue culture bottle 2 to avoid insufficient nutrition in the tissue culture bottle 2 affecting the growth of flowers in the later stage. After loosening the elastic diaphragm 402, negative pressure is formed in the nutrient solution tube 401, so that air is sucked inward by the air inlet pipe 404 to facilitate the next replenishment. The bottle cap 202 will not be opened during the replenishment process, and the sterile environment in the tissue culture bottle 2 will not be destroyed.

[0052] Furthermore, the elastic diaphragm 402 is a hemispherical surface structure, and the elastic diaphragm 402 includes a rubber membrane 4021 and an elastic reed 4022 .

[0053] The rubber membrane 4021 covers the elastic reed 4022 to prevent the elastic reed 4022 from being corroded and rusted. The built-in elastic reed 4022 structure enhances the resilience of the elastic diaphragm 402, ensuring that it can be reset and rebounded after each fluid replenishment, and has a longer service life than pure rubber material.

[0054] Furthermore, the fixing frame 601 is fixedly installed on the top lower end surface of the culture chamber 101, and a slide groove 6011 is set at the bottom of the fixing frame 601. The left and right ends of the pressure rod 602 are provided with sliding rods 6021 that slide with the slide groove 6011. The sleeve 603 is sleeved on the pressure rod 602 and is rotatably connected with it.

[0055] When the bottle rotating mechanism 3 drives the tissue culture bottle 2 to rotate, the sleeve 603 on the surface of the pressure rod 602 intermittently squeezes the elastic diaphragm 402, so that the nutrient solution replenishing mechanism 4 can intermittently replenish the nutrient solution in the culture medium, and the frequency of nutrient solution replenishment can be controlled by controlling the rotation speed of the driving motor 304.

[0056] Furthermore, the lower end surface of the fixing frame 601 is provided with an axial hole 6012, and a rotatably connected adjusting knob 604 is provided in the axial hole 6012. The lower end surface of the adjusting knob 604 is provided with a second screw hole 6041, and the upper end surface of the pressure rod 602 is provided with a screw rod 6022 that cooperates with the second screw hole 6041.

[0057] By turning the adjustment knob 604, the position of the pressure rod 602 is adjusted under the action of the movement of the screw rod 6022. On the one hand, it is convenient to install the tissue culture bottle 2, and on the other hand, it is convenient to adjust the distance between the pressure rod 602 and the bottle cap 202, thereby adjusting the downward pressure distance of the rubber membrane 4021, thereby achieving fine-tuning of the amount of medicine supplemented by the nutrient solution supplement mechanism 4.

[0058] Furthermore, the driving wheel 701 is fixedly mounted on the top of the driving shaft 303, the guide frame 703 is fixedly mounted on the inner end surface of the culture cabinet 1, a guide groove 7031 is provided on the guide frame 703, the knocking rod 702 and the spring 704 are installed in the guide groove 7031 and are slidably connected to the guide frame 703, a first convex tooth 7011 is provided on the side of the driving wheel 701, and a second convex tooth 7021 cooperating with the first convex tooth 7011 is provided on the side of the knocking rod 702.

[0059] When the driving motor 304 drives the driving wheel 701 to rotate through the driving shaft 303, the first protruding tooth 7011 intermittently pushes the second protruding tooth 7021, so that the knocking rod 702 intermittently squeezes the spring 704 into the guide groove 7031. When the first protruding tooth 7011 rotates to separate from the second protruding tooth 7021, the knocking rod 702 knocks on the inner end surface of the culture cabinet 1 under the resetting action of the spring 704, thereby knocking off the water droplets in the culture chamber 101.

[0060] Furthermore, the top and bottom of the culture chamber 101 are inclined structures, and there are two lighting lamps 1012 in each culture chamber 101. The two lighting lamps 1012 are respectively located on the front and rear sides of the bottle pressing mechanism 6, and a drainage hole 1011 is provided on the front end surface of the culture chamber 101.

[0061] By knocking the culture cabinet 1 through the vibrating mechanism 7, the water droplets in the culture chamber 101 slide down the inclined surface, gather at the drainage hole 1011 and discharge downward into the humidification mechanism 5 for re-humidification, so that the humidity in the culture chamber 101 is more uniform. The two groups of lighting lamps 1012 are at different heights, so they complement each other, thereby improving their coverage of the plants in the tissue culture bottle 2 and reducing the blind spots of plant lighting.

[0062] Furthermore, the water tank 501 is fixedly installed on the upper end surface of the bottom of the culture cabinet 1, and the upper end surface of the water tank 501 is provided with a reflux port 5011 corresponding to the position of the drainage hole 1011. The submersible pump 502 is fixedly installed in the water tank 501, and the atomizing nozzle 503 is fixedly installed on the side wall of the culture chamber 101. The submersible pump 502 is connected to the atomizing nozzle 503 through a water supply pipe 504.

[0063] The submersible pump 502 supplies liquid to the atomizing nozzle 503 , and the refluxed water droplets flow back from the drainage hole 1011 to the reflux port 5011 and enter the water tank 501 , and are atomized and humidified again, thereby making the humidity in the culture chamber 101 more uniform.

[0064] When using the device, clean the tissue culture bottle 2 to ensure sterility, add an appropriate amount of initial nutrient solution into the bottle, put the flower tissue into the tissue culture bottle 2, fill the nutrient solution tube 401 with the pre-configured nutrient solution, fix the nutrient solution tube 401 to the lower end surface of the bottle cap 202 through threads, tighten the bottle cap 202 to ensure sealing, install the tissue culture bottle 2 on the turntable 301, ensure that the bottom of the bottle body 201 is aligned with the positioning groove 3011 on the turntable 301, and the transmission groove 2011 at the bottom of the bottle body 201 is slidably matched with the transmission column 3012 on the turntable 301, and adjust the brightness and working time of the lighting lamp 1012 according to the growth needs of the plants. In general, the lighting lamp 1012 should be set to a lighting time of 12-16 hours per day. The target humidity in the culture chamber 101 is set through the control panel of the humidification mechanism 5. In general, the suitable humidity range is 60%-80%. The rotation speed of the bottle rotating mechanism 3 is set through the control panel of the drive motor 304. In general, it starts to rotate slowly at the rooting and germination mouth of the tissue. The rotation speed should be set to 1-2 turns per hour to ensure that the plant tissue receives light evenly. Appropriate adjustments are made as the growth stage changes. By adjusting the adjustment knob 604 of the bottle pressing mechanism 6, the distance between the pressing rod 602 and the bottle cap 202 is set, thereby controlling the amount of nutrient solution added at a time, according to the growth of the plant. According to the long stage and needs, the downward pressing distance of the pressure rod 602 is appropriately adjusted. When the driving motor 304 drives the driving wheel 701 to rotate through the driving shaft 303, the first convex tooth 7011 intermittently pushes the second convex tooth 7021, so that the knocking rod 702 intermittently squeezes the spring 704 into the guide groove 7031. When the first convex tooth 7011 rotates to separate from the second convex tooth 7021, the knocking rod 702 knocks on the inner end surface of the culture cabinet 1 under the resetting action of the spring 704, thereby knocking off the water droplets in the culture chamber 101, and the reflux water droplets flow back to the reflux port 5011 through the drainage hole 1011 and enter the water tank 501, and are atomized and humidified again, so as to make the humidity in the culture chamber 101 more uniform.

[0065] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An induction device for rapid propagation of tissue culture woody flowers, comprising a culture cabinet (1) and a tissue culture bottle (2), characterized in that: The culture cabinet (1) is provided with a bottle rotating mechanism (3), a humidifying mechanism (5), a bottle pressing mechanism (6) and a vibrating mechanism (7), and the tissue culture bottle (2) is provided with a nutrient solution supplementing mechanism (4); The culture cabinet (1) is provided with a culture chamber (101), the culture chamber (101) is provided with an illuminating lamp (1012), the tissue culture bottle (2) is detachably installed in the culture chamber (101), the tissue culture bottle (2) comprises a bottle body (201) and a bottle cap (202), and the bottle cap (202) is threadably connected to the bottle body (201); The bottle rotating mechanism (3) comprises a rotating disk (301), a transmission shaft (302), a driving shaft (303) and a driving motor (304); the driving shaft (303) is mounted on the output shaft of the driving motor (304); the driving shaft (303) drives the rotating disk (301) via the transmission shaft (302); and the tissue culture bottle (2) is mounted on the upper end of the rotating disk (301); The nutrient solution replenishing mechanism (4) comprises a nutrient solution tube (401), an elastic diaphragm (402), a liquid replenishing tube (403) and an air inlet tube (404); the nutrient solution replenishing mechanism (4) is mounted on the bottle cap (202); The humidifying mechanism (5) comprises a water tank (501), a submersible pump (502), an atomizing nozzle (503) and a water supply pipe (504); the humidifying mechanism (5) is installed at the bottom of the inner end surface of the culture cabinet (1); The bottle pressing mechanism (6) comprises a fixing frame (601), a pressing rod (602) and a sleeve (603); the bottle pressing mechanism (6) is fixedly installed on the top of the culture chamber (101); The rapping mechanism (7) comprises a driving wheel (701), a knocking rod (702), a guide frame (703) and a spring (704); the rapping mechanism (7) is installed on the top of the driving shaft (303); The bottle cap (202) is provided with a first screw hole (2022), the elastic diaphragm (402) is fixedly mounted on the upper end surface of the first screw hole (2022), the top outer end surface of the nutrient solution tube (401) is provided with an external thread, the nutrient solution tube (401) is inserted into the first screw hole (2022) and is threadedly connected to the bottle cap (202), the liquid replenishment tube (403) and the air intake tube (404) are mounted on the side wall of the nutrient solution tube (401) and are connected to the nutrient solution tube (401), a one-way valve (4031) is arranged in the liquid replenishment tube (403) and the air intake tube (404), a breathable membrane (2021) is arranged at the center of the bottle cap (202), and the pipe mouth of the liquid replenishment tube (403) is flush with the bottom inner end surface of the liquid replenishment tube (403); When the elastic diaphragm (402) is pressed, the air pressure in the nutrient solution tube (401) increases, thereby squeezing out the nutrient solution in the nutrient solution tube (401) and replenishing the nutrient solution in the tissue culture bottle (2); after the elastic diaphragm (402) is released, negative pressure is formed in the nutrient solution tube (401), so that air is sucked inwardly through the air inlet pipe (404) to facilitate the next replenishment.

2. The induction device for rapid propagation of tissue culture woody flowers as claimed in claim 1, characterized in that: The driving shaft (303) is mounted on the output shaft of the driving motor (304) via a reducer; a first rotating shaft (3013) is provided at the bottom of the rotating disk (301); a worm wheel (3014) is fixedly mounted on the first rotating shaft (3013); a worm (3021) matched with the worm wheel (3014) is provided on the transmission shaft (302); and the transmission shaft (302) and the driving shaft (303) are driven by tooth bevel meshing.

3. The induction device for rapid propagation of tissue culture woody flowers as claimed in claim 1, characterized in that: The upper end surface of the turntable (301) is provided with a positioning groove (3011) corresponding to the shape of the bottom of the bottle body (201), a transmission column (3012) is provided at the center of the positioning groove (3011), and the lower end surface of the bottom of the bottle body (201) is provided with a transmission groove (2011) that slidably cooperates with the transmission column (3012).

4. The induction device for rapid propagation of tissue culture woody flowers as claimed in claim 1, characterized in that: The elastic diaphragm (402) is a hemispherical surface structure, and the elastic diaphragm (402) comprises a rubber membrane (4021) and an elastic reed (4022).

5. The induction device for rapid propagation of tissue culture woody flowers as claimed in claim 1, characterized in that: The fixed frame (601) is fixedly installed on the lower end surface of the top of the culture chamber (101), and a slide groove (6011) is arranged at the bottom of the fixed frame (601). The left and right ends of the pressure rod (602) are provided with sliding rods (6021) that slidably cooperate with the slide groove (6011), and the sleeve (603) is sleeved on the pressure rod (602) and is rotatably connected thereto.

6. The induction device for rapid propagation of tissue culture woody flowers as claimed in claim 5, characterized in that: The lower end surface of the fixing frame (601) is provided with an axial hole (6012), a rotatably connected adjusting knob (604) is provided in the axial hole (6012), a second screw hole (6041) is provided on the lower end surface of the adjusting knob (604), and a screw rod (6022) matching the second screw hole (6041) is provided on the upper end surface of the pressure rod (602).

7. The induction device for rapid propagation of tissue culture woody flowers as claimed in claim 1, characterized in that: The driving wheel (701) is fixedly mounted on the top of the driving shaft (303); the guide frame (703) is fixedly mounted on the inner end surface of the culture cabinet (1); a guide groove (7031) is provided on the guide frame (703); the knocking rod (702) and the spring (704) are mounted in the guide groove (7031) and are slidably connected to the guide frame (703); a first convex tooth (7011) is provided on the side surface of the driving wheel (701); and a second convex tooth (7021) that cooperates with the first convex tooth (7011) is provided on the side surface of the knocking rod (702).

8. The induction device for rapid propagation of tissue culture woody flowers as claimed in claim 1, characterized in that: The top and bottom of the culture chamber (101) are inclined structures, the number of lighting lamps (1012) in each culture chamber (101) is two, and the front end surface of the culture chamber (101) is provided with a drainage hole (1011).

9. The induction device for rapid propagation of tissue culture woody flowers as claimed in claim 1, characterized in that: The water tank (501) is fixedly mounted on the upper end surface of the bottom of the culture cabinet (1); the upper end surface of the water tank (501) is provided with a return port (5011) corresponding to the position of the drainage hole (1011); the submersible pump (502) is fixedly mounted in the water tank (501); the atomizing nozzle (503) is fixedly mounted on the side wall of the culture chamber (101); and the submersible pump (502) is connected to the atomizing nozzle (503) via a water supply pipe (504).

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