Hda isothermal amplification kit for detecting pine wood nematode

Abstract

The application relates to the technical field of isothermal amplification kits, and discloses an HDA isothermal amplification kit for detecting pine wood nematode, which comprises a kit body, a backing plate fixedly installed at the bottom of the kit body, and a storage assembly arranged at the side of the kit body; the storage assembly comprises an adjusting mechanism arranged at the inner wall position of the kit body; a limiting mechanism is installed at the right side wall position of the kit body; an auxiliary mechanism is arranged at the inner wall position of the kit body and connected with one end of the adjusting mechanism; the adjusting mechanism comprises an installation frame fixedly installed at the inner wall of the kit body. The application solves the problem that the existing device adjusts the position of the kit from left to right by rotating a screw rod, but the number of kits is effective, the internal space cannot be fully utilized, and therefore the device has certain limitation problems during use.

Description

Technical Field

[0001] This invention relates to the field of isothermal amplification kit technology, specifically to an HDA isothermal amplification kit for detecting pine wood nematode. Background Technology

[0002] Pine wood nematode is a common parasite of pine trees. The adult nematode is about 1 mm long. The female nematode has a nearly conical tail with a rounded end, while the male nematode has a claw-like tail that curves ventrally. It is transmitted by vector insects such as the pine sawyer beetle, thus causing pine wood nematode disease. Infected pine trees have yellowish-brown or reddish-brown needles, and the entire tree dries up and dies, eventually rotting. The kit is a box used to hold chemical reagents for detecting chemical components, drug residues, virus types, etc. It is commonly used in hospitals and pharmaceutical companies. Therefore, there is a need for an HDA isothermal amplification kit for detecting pine wood nematode.

[0003] For example, Chinese patent CN213951200U discloses an HDA isothermal amplification kit for detecting pine wood nematode. The kit body has a threaded rod inside, a sliding rod inside, a slider on the side surface of the sliding rod, a threaded block on the side surface of the threaded rod, and a heating box on the top of the threaded block. The left side of the kit body is sealed, and the right side has a groove. Unused reagents are located on the left side of the kit body, and used reagents are located inside the groove on the right side of the kit body. This design prevents accidents when handling the kit and protects the reagents.

[0004] However, this patent has certain drawbacks. The device adjusts the position of the reagent kit from left to right by rotating the screw, but the number of reagent kits is limited, making it difficult to make full use of the internal space. This results in certain limitations when the device is used. Summary of the Invention

[0005] The purpose of this invention is to provide an HDA isothermal amplification kit for detecting pine wood nematode, thereby solving the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: an HDA isothermal amplification kit for detecting pine wood nematode, comprising a kit body,

[0007] A pad that is fixedly installed at the bottom of the reagent kit body;

[0008] And a storage component located on the side of the reagent kit body;

[0009] The storage component includes an adjustment mechanism disposed on the inner wall of the reagent kit body;

[0010] A limiting mechanism is installed on the right side wall of the reagent kit body;

[0011] An auxiliary mechanism is provided on the inner wall of the reagent kit body, and the auxiliary mechanism is connected to one end of the adjustment mechanism.

[0012] Preferably, a protective cover is installed on the top side wall of the reagent kit body via a hinge, the bottom of the protective cover is in contact with the top of the reagent kit body, and a handle is fixedly installed on the top of the protective cover.

[0013] Preferably, the adjustment mechanism includes a mounting frame, which is fixedly mounted on the inner wall of the reagent kit body. A second sliding column is slidably mounted on the inner wall of the mounting frame. A second heating box is fixedly mounted on the top of the second sliding column. The bottom of the second heating box is slidably connected to the top of the mounting frame. An adjustment rod is disposed in contact with the side wall of the second sliding column. A rectangular block is fixedly mounted on one end of the adjustment rod. A first screw is mounted on the inner wall of the rectangular block. One end of the first screw extends movably through the inner wall of the reagent kit body to the outer side of the reagent kit body and is driven by a rotating disk. A second screw is slidably mounted on the inner wall of the mounting frame. A first heating box is fixedly mounted on the top of the first sliding column. The bottom of the first heating box is slidably connected to the top of the mounting frame. A second screw is mounted on the inner wall of the first sliding column. One end of the second screw extends movably through the inner wall of the reagent kit body to the outer side of the reagent kit body and is driven by a rotating disk.

[0014] Preferably, the bottom of the rectangular block is slidably connected to the inner wall of the reagent kit body, the side wall of the first screw is threadedly connected to the inner wall of the rectangular block, and the inner wall of the first sliding column is threadedly connected to the side wall of the second screw.

[0015] Preferably, the limiting mechanism includes a mounting plate, which is fixedly mounted on the right side wall of the reagent kit body. A plug rod is slidably mounted on the inner wall of the mounting plate. A pull plate is fixedly mounted on the bottom of the plug rod. A limiting plate is slidably mounted on the top of the plug rod. The side wall of the limiting plate is fixedly connected to the side wall of the protective cover. A connecting spring is fixedly mounted on the bottom of the mounting plate. The bottom of the connecting spring is fixedly connected to the top of the pull plate.

[0016] Preferably, the side wall diameter of the plug rod is the same as the inner wall diameter of the running groove formed on the inner wall of the limiting plate, and the side wall of the plug rod is slidably connected to the inner wall of the circular groove.

[0017] Preferably, the auxiliary mechanism includes a compression spring and a limiting rod. One end of the compression spring is fixedly connected to the inner wall of the mounting frame, and the other end of the compression spring is fixedly mounted with a displacement plate. The side wall of the displacement plate is slidably connected to the inner wall of the mounting frame. The limiting rod is fixedly connected to the inner wall of the heating box, and a partition is slidably mounted on the side wall of the limiting rod.

[0018] Preferably, there are four pads, all of which are of the same shape and size, and the four pads are fixedly installed at the four bottom corners of the reagent kit body.

[0019] This invention provides an HDA isothermal amplification kit for detecting pine wood nematode. It has the following beneficial effects:

[0020] (1) In this invention, the operator can rotate the rotating disk 2 and rotating disk 1 separately as needed. When rotating disk 2 is rotated, it will drive the position of screw 2 to rotate. Since the side wall of screw 2 is threadedly connected to the inner wall of sliding column 1, when screw 2 rotates, it can effectively drive the position of sliding column 1 and heating box 1 to slide on the inner wall of the mounting frame, causing them to move to the right side of the mounting frame. When the operator rotates the position of rotating disk 1, rotating disk 1 drives the position of screw 1 to rotate. Since the side wall of screw 1 is threadedly connected to the inner wall of rectangular block, when screw 1 rotates, it can drive the rectangular block to move to the right side. Under the action of the adjusting rod, the rectangular block drives the position of sliding column 2 and heating box 2 to move. Heating box 2 and sliding column 2 slide from left to right on the mounting frame, which is convenient for the operator to use heating box 2. This solves the problem that the existing device adjusts the position of the reagent kit from left to right by rotating the screw, but the number of reagent kits is limited, and it is difficult to make full use of its internal space, which leads to certain limitations when the device is used.

[0021] (2) When the operator needs to use the heating box and needs to open the protective cover, the operator can directly pull down the pull plate. The pull plate drives the plug rod to slide on the inner wall of the limiting plate. The pull plate will also pull one end of the connecting spring. When one end of the plug rod is disengaged from the inner wall of the limiting plate, the operator can pull the handle. The handle will drive the protective cover to rotate on the top of the reagent kit body. The limiting mechanism can effectively limit the position of the protective cover.

[0022] (3) The present invention can make the sliding column 2 slide to its original position effectively without being squeezed by the adjusting rod by means of the displacement plate and the compression spring. By designing a partition on the side wall of the limiting rod, different reagents can be effectively separated and stored, making it easier for operators to use the device. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the external structure of the present invention;

[0024] Figure 2 This is a schematic diagram of the side structure of the present invention;

[0025] Figure 3 This is a top view cross-sectional structural diagram of the present invention;

[0026] Figure 4 This is a schematic diagram of the adjustment mechanism of the present invention;

[0027] Figure 5 For the present invention Figure 1 A magnified view of part A in the image.

[0028] In the diagram: 1. Reagent kit body; 2. Pad; 3. Protective cover; 4. Storage components; 41. Adjustment mechanism; 411. Mounting frame; 412. Screw 1; 413. Rotating disk 1; 414. Rotating disk 2; 415. Rectangular block; 416. Adjustment rod; 417. Sliding column 1; 418. Screw 2; 419. Heating box 1; 4110. Heating box 2; 4111. Sliding column 2; 42. Limiting mechanism; 421. Limiting plate; 422. Insertion rod; 423. Mounting plate; 424. Pull plate; 425. Connecting spring; 43. Auxiliary mechanism; 431. Compression spring; 432. Displacement plate; 433. Limiting rod; 434. Partition; 5. Handle. Detailed Implementation

[0029] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0030] Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the invention, and should not be construed as limiting the invention.

[0031] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0032] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0033] To provide a clearer understanding of the technical features, objectives, and effects of the present invention, specific embodiments of the present invention will now be described with reference to the accompanying drawings.

[0034] Example 1: A preferred embodiment of the HDA isothermal amplification kit for detecting pine wood nematode provided by the present invention is as follows: Figures 1 to 5 As shown: An HDA isothermal amplification kit for detecting pine wood nematode includes a kit body 1. A protective cover 3 is hinged to the top side wall of the kit body 1. The bottom of the protective cover 3 contacts the top of the kit body 1. A handle 5 is fixedly installed on the top of the protective cover 3, allowing the operator to open the protective cover 3.

[0035] Four pads 2 are fixedly installed at the bottom of the reagent kit body 1. The four pads 2 are all the same shape and size. The four pads 2 are fixedly installed at the four corners of the bottom of the reagent kit body 1.

[0036] And the storage component 4 located on the side of the reagent kit body 1;

[0037] The storage component 4 includes an adjustment mechanism 41 located on the inner wall of the reagent kit body 1;

[0038] A limiting mechanism 42 is installed on the right side wall of the reagent kit body 1;

[0039] An auxiliary mechanism 43 is provided on the inner wall of the reagent kit body 1, and the auxiliary mechanism 43 is connected to one end of the adjustment mechanism 41.

[0040] The adjustment mechanism 41 includes a mounting frame 411, which is fixedly mounted on the inner wall of the reagent kit body 1. A sliding column 4111 is slidably mounted on the inner wall of the mounting frame 411. A heating box 4110 is fixedly mounted on the top of the sliding column 4111. The bottom of the heating box 4110 is slidably connected to the top of the mounting frame 411. An adjustment rod 416 is disposed in contact with the side wall of the sliding column 4111. A rectangular block 415 is fixedly mounted on one end of the adjustment rod 416. A screw 412 is mounted on the inner wall of the rectangular block 415. One end of the device extends through the inner wall of the reagent kit body 1 to the outer side of the reagent kit body 1, and is equipped with a rotating disk 413. A sliding column 417 is slidably installed on the inner wall of the mounting frame 411. A heating box 419 is fixedly installed on the top of the sliding column 417. The bottom of the heating box 419 is slidably connected to the top of the mounting frame 411. A screw 418 is installed on the inner wall of the sliding column 417. One end of the screw 418 extends through the inner wall of the reagent kit body 1 to the outer side of the reagent kit body 1, and is equipped with a rotating disk 414.

[0041] In this embodiment, the bottom of the rectangular block 415 is slidably connected to the inner wall of the reagent kit body 1, the side wall of the screw 412 is threadedly connected to the inner wall of the rectangular block 415, and the inner wall of the sliding column 417 is threadedly connected to the side wall of the screw 418.

[0042] In the specific implementation process, the operator can rotate the rotating disk 2 414 and the rotating disk 1 413 separately as needed. When rotating the rotating disk 2 414, it will drive the position of the screw 2 418 to rotate. Since the side wall of the screw 2 418 is threadedly connected to the inner wall of the sliding column 1 417, when the screw 2 418 rotates, it can effectively drive the position of the sliding column 1 417 and the heating box 1 419 to slide on the inner wall of the mounting frame 411, causing it to move to the right side of the mounting frame 411. The operator can then rotate the rotating disk 2 414... When the rotating disk 413 rotates, the rotating disk 413 drives the rotating screw 412 to rotate. Since the side wall of the screw 412 is threadedly connected to the inner wall of the rectangular block 415, the rotating screw 412 can drive the rectangular block 415 to move to the right. Under the action of the adjusting rod 416, the rectangular block 415 drives the sliding column 4111 and the heating box 4110 to move. The heating box 4110 and the sliding column 4111 slide from left to right on the mounting frame 411, which makes it convenient for the operator to use the heating box 4110.

[0043] Example 2: Based on Example 1, a preferred embodiment of the HDA isothermal amplification kit for detecting pine wood nematode provided by the present invention is as follows: Figures 1 to 5As shown: The limiting mechanism 42 includes a mounting plate 423, which is fixedly mounted on the right side wall of the reagent kit body 1. A plug rod 422 is slidably mounted on the inner wall of the mounting plate 423. A pull plate 424 is fixedly mounted on the bottom of the plug rod 422. A limiting plate 421 is slidably mounted on the top of the plug rod 422. The side wall of the limiting plate 421 is fixedly connected to the side wall of the protective cover 3. A connecting spring 425 is fixedly mounted on the bottom of the mounting plate 423. The bottom of the connecting spring 425 is fixedly connected to the top of the pull plate 424.

[0044] In this embodiment, the side wall diameter of the plug rod 422 is the same as the inner wall diameter of the running groove formed on the inner wall of the limiting plate 421, and the side wall of the plug rod 422 is slidably connected to the inner wall of the circular groove.

[0045] In the specific implementation process, when the operator uses the device, when the heating box needs to be used, and when the protective cover 3 needs to be opened, the operator can directly pull down the pull plate 424. The pull plate 424 drives the plug rod 422 to slide on the inner wall of the limiting plate 421. The pull plate 424 will also pull one end of the connecting spring 425. When one end of the plug rod 422 is disengaged from the inner wall of the limiting plate 421, the operator can pull the handle 5. The handle 5 will drive the protective cover 3 to rotate on the top of the reagent kit body 1. The position of the protective cover 3 can be effectively limited by the limiting mechanism 42.

[0046] Example 3: Based on Examples 1 and 2, a preferred embodiment of the HDA isothermal amplification kit for detecting pine wood nematodes provided by the present invention is as follows: Figures 1 to 5 As shown: The auxiliary mechanism 43 includes a compression spring 431 and a limiting rod 433. One end of the compression spring 431 is fixedly connected to the inner wall of the mounting frame 411, and the other end of the compression spring 431 is fixedly mounted with a displacement plate 432. The side wall of the displacement plate 432 is slidably connected to the inner wall of the mounting frame 411. The limiting rod 433 is fixedly connected to the inner wall of the heating box 419, and a partition 434 is slidably mounted on the side wall of the limiting rod 433.

[0047] In the specific implementation process, the displacement plate 432 and the compression spring 431 can make the sliding column 4111 slide effectively to its original position without being squeezed by the adjusting rod 416. By designing a partition 434 on the side wall of the limiting rod 433, different reagents can be effectively separated and stored.

[0048] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

[0049] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. An HDA isothermal amplification kit for detecting pine wood nematode, comprising the kit body (1). A pad (2) is fixedly installed at the bottom of the reagent kit body (1); and a storage component (4) disposed on the side of the reagent kit body (1); characterized in that: The storage component (4) includes an adjustment mechanism (41) disposed on the inner wall of the reagent kit body (1). The adjustment mechanism (41) includes a mounting frame (411), which is fixedly mounted on the inner wall of the reagent kit body (1). A sliding column two (4111) is slidably mounted on the inner wall of the mounting frame (4111). A heating box two (4110) is fixedly mounted on the top of the sliding column two (4111). The bottom of the heating box two (4110) is slidably connected to the top of the mounting frame (411). An adjustment rod (416) is provided in contact with the side wall of the sliding column two (4111). A rectangular block (415) is fixedly mounted on one end of the adjustment rod (416). A screw one (412) is mounted on the inner wall of the rectangular block (415). One end of the screw one (412) extends movably through the inner wall of the reagent kit body (1) to the outer side of the reagent kit body (1), and a drive is installed. The moving disk (413) has a sliding column (417) slidably installed on the inner wall of the mounting frame (411). A heating box (419) is fixedly installed on the top of the sliding column (417). The bottom of the heating box (419) is slidably connected to the top of the mounting frame (411). A screw (418) is installed on the inner wall of the sliding column (417). One end of the screw (418) extends through the inner wall of the reagent kit body (1) to the outside of the reagent kit body (1) and is driven by a rotating disk (414). The bottom of the rectangular block (415) is slidably connected to the inner wall of the reagent kit body (1). The side wall of the screw (412) is threadedly connected to the inner wall of the rectangular block (415). The inner wall of the sliding column (417) is threadedly connected to the side wall of the screw (418). A limiting mechanism (42) is installed on the right side wall of the reagent kit body (1). An auxiliary mechanism (43) is provided on the inner wall of the reagent kit body (1), and the auxiliary mechanism (43) is connected to one end of the adjustment mechanism (41); The auxiliary mechanism (43) includes a compression spring (431) and a limiting rod (433). One end of the compression spring (431) is fixedly connected to the inner wall of the mounting frame (411), and the other end of the compression spring (431) is fixedly installed with a displacement plate (432). The side wall of the displacement plate (432) is slidably connected to the inner wall of the mounting frame (411). The limiting rod (433) is fixedly connected to the inner wall of the heating box (419), and a partition plate (434) is slidably installed on the side wall of the limiting rod (433).

2. The HDA isothermal amplification kit for detecting pine wood nematode according to claim 1, characterized in that: The top side wall of the reagent kit body (1) is fitted with a protective cover (3) by a hinge. The bottom of the protective cover (3) is in contact with the top of the reagent kit body (1). A handle (5) is fixedly installed on the top of the protective cover (3).

3. The HDA isothermal amplification kit for detecting pine wood nematode according to claim 1, characterized in that: The limiting mechanism (42) includes a mounting plate (423), which is fixedly mounted on the right side wall of the reagent kit body (1). A plug rod (422) is slidably mounted on the inner wall of the mounting plate (423). A pull plate (424) is fixedly mounted on the bottom of the plug rod (422). A limiting plate (421) is slidably mounted on the top of the plug rod (422). The side wall of the limiting plate (421) is fixedly connected to the side wall of the protective cover (3). A connecting spring (425) is fixedly mounted on the bottom of the mounting plate (423). The bottom of the connecting spring (425) is fixedly connected to the top of the pull plate (424).

4. The HDA isothermal amplification kit for detecting pine wood nematode according to claim 3, characterized in that: The side wall diameter of the plug rod (422) is the same as the inner wall diameter of the running groove opened on the inner wall of the limiting plate (421), and the side wall of the plug rod (422) is slidably connected to the inner wall of the circular groove.

5. The HDA isothermal amplification kit for detecting pine wood nematode according to claim 1, characterized in that: There are four pads (2), all of which are the same size and shape. The four pads (2) are fixedly installed at the four bottom corners of the reagent kit body (1).

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