Picea flower monochamus alternatus attractant and use method thereof

Abstract

The invention provides a picea flower monochamus alternatus attractant and a use method thereof.The attractant comprises plant source pheromone and insect pheromone, the chemical components are mixed according to a certain dosage and proportion and then dropwise added to slow-release carriers such as a slow-release bottle, a slow-release bag and a capillary tube, and a picea flower monochamus alternatus lure is prepared; the insecticidal composition can be used for trapping a large number of black longhorn beetles of picea flowers and has the characteristics of remarkable trapping effect and longer lasting period. The attractant is simple to prepare, good in attraction effect and not prone to generating resistance, the formula is a combination of host plant volatile matter and insect pheromone, the attractant is harmless to people and livestock and free of pollution to the environment, a practical pollution-free monitoring and prevention technical means is provided for large-scale trapping and killing of the black longhorn beetles in forests, forest resources are protected, and the economic benefits are increased. The ecological balance is maintained, the application value is very good, and the sustainable development of forestry is promoted.

Description

Technical Field

[0001] The invention relates to the field of attractants, and in particular to a spruce-flowered Monochamus alternatus attractant and a use method thereof. Background Art

[0002] Spruce-flowered Monochamus saltuarius belongs to the family Cerambycidae, subfamily Lamiinae, order Coleoptera, and is a major forestry pest. In addition to directly harming important economic trees such as Korean pine, spruce, Pinus sylvestris, Abies, and Pinus tabulaeformis, it can also spread pine wood nematodes through feeding and laying eggs, causing the "cancer" of pine trees - pine wood nematode disease, which seriously threatens the health of forest trees and economic interests. At present, the main means of control of spruce-flowered Monochamus saltuarius are physical and chemical control. Among them, physical methods such as mechanical control, wood trapping and manual killing, although less environmental pollution, have low control efficiency and high labor costs; chemical methods such as spraying pesticides and trunk injection, although quick to take effect, are often accompanied by environmental pollution, affecting non-target organisms, and can also induce pest resistance. In view of the defects and challenges of existing prevention and control technologies, environmentally friendly and highly effective pheromone trapping technology has received increasing attention. By studying the chemical communication mechanism between plants and insects, using plant-derived pheromones, insect pheromones and other chemical substances in conjunction with traps, precise trapping of pests can be achieved. Therefore, finding a pheromone formula that is suitable and can effectively attract Monochamus alternatus is of great significance for green prevention and control of this pest.

[0003] Prior art 1:

[0004] The patent "Method for preventing and controlling pine wood nematode disease by predicting and capturing pine and spruce beetles in large quantities" (patent number: CN201480048045.41) invented by Park Yong-sik et al., its attractant formula consists of the following parts: 2-(undecy)-1-ethanol, α-pinene, ethanol, (±)-3-carene, isophthalic acid, isophthalic acid dienol, n-pentacosane, n-nonacosane, which are fully mixed in a certain weight ratio, usually in a weight ratio of 0.01~1: 1~400: 1~400: 1~400: 0.01~3: 0.01~3: 0.0001~0.010: 0.0001~0.010.

[0005] In the above-mentioned prior art, the proportion of pheromone substances is unreasonable, which affects the trapping effect. Summary of the invention

[0006] In view of this, the technical problem to be solved by the present invention is to provide a spruce-flowered Monochamus alternatus attractant and a method of using the same, which can effectively improve the trapping effect.

[0007] The technical solution of the present invention is achieved in this way:

[0008] A spruce-flowered Monochamus alternatus attractant comprises a plant-derived pheromone and an insect aggregation pheromone, wherein the plant-derived pheromone comprises 135-180 parts by volume of β-pinene and 0-45 parts by volume of ethanol; the insect aggregation pheromone comprises 0.02-0.2 parts by volume of 2-(undecanyloxy)-1-ethanol and 0.02-0.04 parts by volume of 1-(1H-pyrrol-2-yl)-1,2-propanedione.

[0009] Preferably, the plant-derived pheromone comprises 135 parts by volume of β-pinene and 45 parts by volume of ethanol.

[0010] Preferably, the insect aggregation pheromone includes 0.1 parts by volume of 2-(undecanyloxy)-1-ethanol and 0.04 parts by volume of 1-(1H-pyrrol-2-yl)-1,2-propanedione.

[0011] The present invention also provides a method for using the spruce-flowered Monochamus alternatus attractant, wherein the plant-derived pheromone is released through a slow-release bottle, and the insect aggregation pheromone is released through a slow-release bag.

[0012] Preferably, the wall thickness of the sustained-release bottle is 0.2-0.7 mm, and the wall thickness of the sustained-release bag is 0.04-0.12 mm.

[0013] Preferably, the wall thickness of the sustained-release bottle is 0.4 mm, and the wall thickness of the sustained-release bag is 0.08 mm.

[0014] The present invention also proposes a method for using a spruce-flowered Monochamus alternatus attractant, wherein the plant-derived pheromone is released through a sustained-release bottle, 2-(undecanyloxy)-1-ethanol is released through a sustained-release bag, and 1-(1H-pyrrol-2-yl)-1,2-propanedione is released through a capillary.

[0015] Preferably, the wall thickness of the sustained-release bottle is 0.4 mm, the wall thickness of the sustained-release bag is 0.08 mm, and the wall thickness of the capillary tube is 0.03 mm.

[0016] The invention provides a spruce-flowered Monochamus alternatus attractant and a method for using the same. The attractant comprises plant-derived pheromones and insect pheromones. The chemical components are mixed in certain dosages and proportions, and then dripped onto a sustained-release carrier such as a sustained-release bottle, a sustained-release bag, and a capillary tube to prepare a spruce-flowered Monochamus alternatus attractant core. The attractant can trap a large number of spruce-flowered Monochamus alternatus and has the characteristics of significant trapping effect and longer lasting effect. DETAILED DESCRIPTION

[0017] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0018] In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top / bottom" and the like indicating directions or positional relationships are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0019] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "mounted / connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0020] Source of reagents:

[0021]

[0022]

[0023] We have carried out example studies on different attractant formulations and sustained-release technologies, and the example schemes are designed as follows:

[0024] Example 1

[0025] The test site was in the state-owned Cangshi Forest Farm in Qingyuan Manchu Autonomous County, Fushun City, Liaoning Province (41°59'N, 124°31'E). We put plant-derived pheromones (α-pinene, β-pinene, myrcene, 3-carene, phellandrene, D-limonene, camphor, terpinolene and ethanol) into 200mL sustained-release bottles according to certain volumes, and put the aggregation pheromone of spruce-flowered Monochamus alternatus (2-undecanyloxy-1-ethanol) and beetle pheromones (beetle-enol and beetle-dienoyl alcohol) on sustained-release tablets, which were placed in sustained-release bags. The sustained-release bags and bottles were hung in traps, which were hung on the tree at a height of about 1.5m from the ground. Five traps were set for each formula, randomly distributed, with an interval of 20m between each trap. The trap was set up on July 9, 2022. From July 12 to July 24, 2022, the number of Monochamus alternatus in the trap was counted every 3 days, and the trap collection bottle was cleaned. The location of different compounds was changed during each survey to reduce the influence of geographical location on the experimental results. The following forest trapping test methods were the same as above.

[0026] Table 1 A forest trapping experiment of single plant-derived pheromones on Monochamus alternatus (Qingyuan Manchu Autonomous County, Fushun, Liaoning, 2022, 7.9-7.24, N=5)

[0027] formula Element Total number of traps (head) 1 Blank control 1 2 α-Pinene 180mL 44 3 β-pinene 180mL 50 4 Myrcene 180mL 29 5 3-Carene 180mL 55 6 Phellandrene 180mL 32 7 D-Limonene 180mL 4 8 Camphor 180mL 12 9 Terpinolene 180mL 5

[0028] The results of Example 1 show that plant-derived pheromones have a significant effect on the trapping effect, among which 3-carene has the best trapping effect, followed by β-pinene and α-pinene, while the control group without adding plant-derived pheromones has the worst trapping effect. The difference is very significant, which shows that plant-derived pheromones are a very important substance in the attractant of spruce-flowered Monochamus alternatus, and at a certain added dosage, the trapping effect can be significantly increased.

[0029] Example 2

[0030] Table 2 Forest trapping test of ethanol combined with plant-derived substances on Monochamus alternatus (Qingyuan Manchu Autonomous County, Fushun, Liaoning, 2023, 5.11-5.23, N=5)

[0031] formula Element Total number of traps (head) 1 α-Pinene (180mL) 12 2 β-Pinene (180mL) 16 3 3-Carene (180mL) 19 4 α-Pinene (135 mL) + ethanol (45 mL) 21 5 β-pinene (135 mL) + ethanol (45 mL) 45 6 3-Carene (135mL) + ethanol (45mL) 27

[0032] The results of Example 2 show that after adding a certain amount of ethanol to Formulas 1, 2 and 3, the trapping results of Formulas 4, 5 and 6 are significantly improved compared with the original formulas without ethanol, which shows that fermentation substance ethanol has a good synergistic effect on terpenes and is an important synergistic substance. Among them, Formula 5 has the best trapping effect, which is significantly better than Formulas 4 and 6.

[0033] Example 3

[0034] Table 3 Forest trapping experiment of different doses of longicorn aggregation pheromone on Monochamus alternatus (Qingyuan Manchu Autonomous County, Fushun, Liaoning, 2023, 5.29-6.13, N=5)

[0035]

[0036] The results of Example 3 show that, first, compared with Formulation 1, Formulations 2, 3, 4 and 5, after adding different doses of the aggregation pheromone 2-(undedecyloxy)-1-ethanol of spruce-flowered Monochamus alternatus, all have significant effects on the trapping results, among which Formulation 4 with the addition of 100 μL 2-(undedecyloxy)-1-ethanol has the best trapping effect, followed by Formulation 5 with the addition of 200 μL 2-(undedecyloxy)-1-ethanol. This shows that aggregation pheromones play a very important role in spruce-flowered Monochamus alternatus attractants, and that plant-derived pheromones and insect pheromones have a good synergistic effect.

[0037] In addition, the attraction of aggregation pheromones to spruce-flowered Monochamus alternatus is related to the concentration of aggregation pheromones. The trapping effect of the compound attractant core with 100 μL of 2-(undecanyloxy)-1-ethanol on spruce-flowered Monochamus alternatus is higher than that with 20 and 50 μL, but there is no significant difference in the trapping effect compared with the compound attractant core with 200 μL of 2-(undecanyloxy)-1-ethanol, indicating that the attracting effect of 2-(undecanyloxy)-1-ethanol has a critical value and will not continue to increase with the increase of dosage. Considering the cost and effect of forest application, it is more appropriate to use 100 μL of 2-(undecanyloxy)-1-ethanol as a synergist for plant-based compound attractants.

[0038] Example 4

[0039] Table 4 Forest trapping experiment of different doses of longicorn pheromones on Monochamus alternatus (Qingyuan Manchu Autonomous County, Fushun, Liaoning, 2023, 7.12-7.24, N=5)

[0040]

[0041] The results of Example 4 show that compared with Formula 1, Formulas 2 and 3, after adding different doses of longhorn beetle pheromone 1-(1H-pyrrole-2-yl)-1,2-propanedione, significantly improved the trapping effect on spruce-flowered Monochamus alternatus, which indicates that longhorn beetle pheromone 1-(1H-pyrrole-2-yl)-1,2-propanedione can synergistically increase the trapping effect on spruce-flowered Monochamus alternatus. Among them, Formula 3 (adding 40 μL 1-(1H-pyrrole-2-yl)-1,2-propanedione) has the best synergistic effect.

[0042] Example 5

[0043] Table 5 Forest trapping test of slow-release materials with different thicknesses (Qingyuan Manchu Autonomous County, Fushun, Liaoning, 2023, 7.28-8.24, N=5)

[0044]

[0045]

[0046] Note: β-pinene (135 mL) + ethanol (45 mL) was released in a sustained-release bottle, and 2-(undecanyloxy)-1-ethanol (100 μL) + 1-(1H-pyrrol-2-yl)-1,2-propanedione (40 μL) was released in a sustained-release bag.

[0047] The results of Example 5 show that the wall thickness of the sustained-release bottle and the thickness of the sustained-release bag have a significant effect on the trapping results. Among them, the best trapping effect is achieved when β-pinene (135 mL) + ethanol (45 mL) is used in a sustained-release bottle with a wall thickness of 0.40 mm and 2-(undecanyloxy)-1-ethanol (100 μL) + 1-(1H-pyrrol-2-yl)-1,2-propanedione (40 μL) is used in a sustained-release bag with a bag thickness of 0.08 mm.

[0048] Example 6

[0049] Table 6 Forest trapping test with capillaries of different thicknesses (Qingyuan Manchu Autonomous County, Fushun, Liaoning, 2023, 8.26-9.15, N=5)

[0050]

[0051] Note: β-pinene (135 mL) + ethanol (45 mL) was released by sustained-release bottle, 2-(undecanyloxy)-1-ethanol (100 μL) was released by sustained-release bag, and 1-(1H-pyrrol-2-yl)-1,2-propanedione (40 μL) was released by capillary tube.

[0052] The results of Example 6 show that after 1-(1H-pyrrole-2-yl)-1,2-propanedione (40 μL) was released using a capillary, treatment 2 with a PVC capillary with a wall thickness of 0.03 mm had the best trapping effect, which was significantly better than 0.02 mm, 0.04 mm and 0.05 mm.

[0053] The attractant is a spruce-flowered Monochamus alternatus attractant and a method for using the attractant. The attractant comprises plant-derived pheromones and insect pheromones, wherein the plant-derived pheromones comprise 75 to 100 parts by volume of β-pinene and 0 to 25 parts by volume of ethanol; the aggregation pheromones comprise 20 to 200 μL / attractant core of 2-(undecanyloxy)-1-ethanol and 20 to 40 μL / attractant core of 1-(1H-pyrrol-2-yl)-1,2-propanedione.

[0054] After the above chemical components are mixed in a certain dosage and proportion, they are added dropwise to a slow-release carrier such as a slow-release bottle, a slow-release bag and a capillary tube to prepare a spruce-flowered Monochamus alternatus attractant core, which can trap a large number of spruce-flowered Monochamus alternatus, and has the characteristics of significant trapping effect and longer lasting effect. The spruce-flowered Monochamus alternatus attractant provided by the present invention is simple to prepare, has a good luring effect, is not easy to produce resistance, and is formulated as a combination of host plant volatiles and insect pheromones. It is harmless to humans and animals and does not pollute the environment. It provides practical pollution-free monitoring and prevention and control technical means for large-scale trapping of spruce-flowered Monochamus alternatus in the forest, protects forest resources, maintains ecological balance, has good application value, and promotes the sustainable development of forestry

[0055] Finally, it should be noted that the above is only a preferred embodiment of the present invention, which is only used to illustrate the technical solution of the present invention, and is not used to limit the protection scope of the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention are included in the protection scope of the present invention.

Claims

1. A spruce beetle attractant, characterized in that: The invention comprises plant-derived pheromones and insect aggregation pheromones. The plant-derived pheromones comprise 135 to 180 parts by volume of β-pinene and 0 to 45 parts by volume of ethanol; the insect aggregation pheromones comprise 0.02 to 0.2 parts by volume of 2-(undecanyloxy)-1-ethanol and 0.02 to 0.04 parts by volume of 1-(1H-pyrrol-2-yl)-1,2-propanedione.

2. The method for attracting Monochamus alternatus from spruce flowers as claimed in claim 1, characterized in that: The plant-derived pheromone comprises 135 parts by volume of β-pinene and 45 parts by volume of ethanol.

3. The Monochamus alternatus attractant according to claim 1, characterized in that: The insect aggregation pheromone includes 0.1 parts by volume of 2-(undecanyloxy)-1-ethanol and 0.04 parts by volume of 1-(1H-pyrrol-2-yl)-1,2-propanedione.

4. The method for using the Monochamus alternatus attractant according to any one of claims 1 to 3, characterized in that: The plant-derived pheromone is released through a slow-release bottle, and the insect aggregation pheromone is released through a slow-release bag.

5. The method for using the Monochamus alternatus attractant according to claim 4, characterized in that: The wall thickness of the sustained-release bottle is 0.2-0.7 mm, and the wall thickness of the sustained-release bag is 0.04-0.12 mm.

6. The method for using the Monochamus alternatus attractant according to claim 5, characterized in that: The wall thickness of the sustained-release bottle is 0.4 mm, and the wall thickness of the sustained-release bag is 0.08 mm.

7. The method for using the Monochamus alternatus attractant according to any one of claims 1 to 3, characterized in that: The plant-derived pheromone is released through a slow-release bottle, 2-(undecanyloxy)-1-ethanol is released through a slow-release bag, and 1-(1H-pyrrol-2-yl)-1,2-propanedione is released through a capillary tube.

8. The method for using the Monochamus alternatus attractant according to claim 7, characterized in that: The wall thickness of the sustained-release bottle is 0.4 mm, the wall thickness of the sustained-release bag is 0.08 mm, and the wall thickness of the capillary is 0.03 mm.

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