Preparation method and application of compound grease simulating breast milk lipid composition and structure

Abstract

The invention belongs to the technical field of infant formula milk powder, and discloses a preparation method and application of compound grease simulating breast milk lipid composition and structure. The method specifically comprises the following steps: (1) determining the content of corresponding total fatty acids and triglycerides in grease raw materials and phospholipids by taking main fatty acids and triglycerides with the content of more than 1% in breast milk fat as templates; (2) simulating through a computer to obtain different raw material proportions; (3) scoring the grease raw materials and phospholipids in different proportions, and selecting the raw material proportion with the highest score; (4) preparing phospholipid into a solution with purified water; (5) adding a grease raw material into the prepared solution to prepare an emulsion; and (6) homogenizing to obtain the compound grease simulating breast milk lipid composition and structure. Compared with compound grease of conventional goat milk-based infant formula milk powder, the composition and structure of fat of the product are humanized, and fat metabolism and digestion of infants are facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of infant formula milk powder, and more particularly to a method for preparing a compound oil added to goat milk-based infant formula milk powder and simulating the lipid composition and structure of breast milk. Background Art

[0002] Breast milk is the golden food source for newborns. Breast milk fat, as the core molecule of biological reactions, plays an important role in energy storage, cell membrane formation and signal transmission. Breast milk fat globules are composed of fatty acids and glycerides as the core, and are wrapped in three layers of phospholipid membranes. Proteins, cholesterol and nearly 100 kinds of bioactive substances are embedded between the membranes to form the breast milk fat globule membrane, which plays an important role in maintaining the stability of the oil-water interface and providing enzyme action sites. With the accelerated pace of life, breastfeeding can not always meet the needs of infants in time. Therefore, infant formula milk powder, as a substitute for breast milk, came into being. However, the particle size of the fat droplets of infant formula milk powder is smaller, and it lacks the wrapping of breast milk fat globule membrane. It is significantly different from breast milk in lipid composition and structure, which directly affects the lipid digestion and utilization rate of infants.

[0003] The breast milkization of infant formula fat has been listed as a research focus. At present, the simulation of lipid composition of bovine milk-based infant formula is relatively mature, but the simulation of lipid composition applied to the production of goat milk-based infant formula is still imperfect. The preparation of compound oils that simulate the lipid composition and structure of breast milk is conducive to narrowing the difference between lipids in infant formula and breast milk fat. Using oils from a variety of different sources as raw materials, comprehensive component simulation of infant formula lipids will help improve the utilization and digestibility of infant formula fat droplets in infants. However, it only stays at the fatty acid level and ignores the simulation of the important triglyceride composition.

[0004] Therefore, based on the integrated analysis of breast milk lipid composition, it is of great significance to develop a compound oil that is closer to breast milk fat in lipid composition and structure as an added ingredient for goat milk-based infant formula. Summary of the invention

[0005] In view of the above problems, the present invention provides a compound oil formula and preparation method that simulates the lipid composition and structure of breast milk, which is suitable for the preparation of goat milk-based infant formula milk powder. Based on the fat content in every 100 mL of infant formula milk powder, vegetable oil, OPO structured fat, phospholipids and goat milk fat are added, and the compound oil that simulates the lipid composition and structure of breast milk is prepared by adjusting the order of adding raw materials.

[0006] One of the purposes of the present invention is to provide a method for preparing a compound oil simulating the lipid composition and structure of breast milk, comprising the following steps:

[0007] (1) Using the main fatty acids and triglycerides with a content greater than 1% in breast milk fat as a template, the corresponding total fatty acid and triglyceride contents in oil raw materials and phospholipids were determined;

[0008] (2) Computer simulation was performed using Matlab software to obtain different raw material ratios;

[0009] (3) Using the scoring principle, score the oil raw materials and phospholipids with different ratios, and select the raw material ratio with the highest score;

[0010] (4) preparing the phospholipid into a 2% (W / V) solution with purified water and stirring magnetically overnight to completely dissolve it;

[0011] (5) adding the oil raw material to the solution prepared in 4) and shearing to obtain an emulsion;

[0012] (6) homogenizing the emulsion to obtain a compound oil that simulates the lipid composition and structure of breast milk.

[0013] Furthermore, in step (1), there are 8 main fatty acids with a content greater than 1% in the breast milk fat, including C10:0, C12:0, C14:0, C16:0, C16:1, C18:0, C18:1, and C18:2.

[0014] Furthermore, in step (1), there are 6 types of triglycerides with a content greater than 1% in the breast milk fat, including OPL, OPO, OOO, PPL, PPO, and LLL.

[0015] Furthermore, the oil raw material in step (1) includes vegetable oil, OPO structured oil, and goat milk.

[0016] Further preferably, the vegetable oil includes one or more of soybean oil, corn oil, sunflower oil, palm oil, coconut oil, linseed oil, walnut oil and rapeseed oil.

[0017] Furthermore, in step (1), the phospholipids include one or more of egg yolk lecithin and soybean lecithin.

[0018] Furthermore, in step (3), the scoring principle is:

[0019] The scoring principle is the same as the crude oil physical mixing simulation conditions:

[0020] G=G1+G2

[0021]

[0022] Among them, G is the total score of 100 points, G1 and G2 are the total scores of conditions 1 (triglycerides) and 2 (total fatty acids), 50 and 50 points respectively.

[0023]

[0024] in, is the weight of breast milk triglyceride TAG / total fatty acids FAs; Di is the average value of breast milk TAG / FAs; Ci is the floating coefficient; Bi is the content value of breast milk TAG / FAs after mixing in different proportions; Ai is the range value of breast milk TAG;

[0025] When Bi>Ai maximum value, Ai takes the breast milk range Max; when Bi<Ai minimum value, Ai takes the breast milk range Min; when Bi is within the Ai range, Ci=0.

[0026] The beneficial effect of the scoring principle of the present invention is that all the obtained oil raw material ratios are scored by the scoring principle, and the closer the obtained score is to 100, the closer the component composition of the ratio is to breast milk, thereby obtaining the optimal compound oil ratio.

[0027] Preferably, the shearing in step (5) is high-speed shearing at 10,000 r / min for 10 min.

[0028] The technical concept of adding phospholipids to the oil raw materials of the present invention is as follows: the laboratory preliminary test results show that the mixing order in the current production of infant formula milk powder (simultaneous homogenization of non-thermosensitive milk base materials) is not conducive to the adsorption of phospholipids on the surface of fat globules to form a structure similar to the breast milk fat globule membrane. Therefore, the present invention adjusts the filler order, preferentially mixes and homogenizes phospholipids with the oil raw materials to form a phospholipid membrane. Then, it is homogenized with other milk base materials to enhance the adsorption competitiveness of phospholipids, so that a structure similar to the breast milk fat globule membrane can be better formed.

[0029] Preferably, the homogenization in step (6) is performed at 25 MPa for 3-5 times.

[0030] The second object of the present invention is to provide a compound oil simulating the lipid composition and structure of breast milk for use in goat milk-based infant formula.

[0031] It can be seen from the above technical solution that, compared with the prior art, the beneficial effects achieved by the present invention are:

[0032] 1) Compared with the compound fats of conventional goat milk-based infant formula, this product adds computer-simulated vegetable oils, OPO structured oils\fats, phospholipids and goat milk fat, making the compound fats closer to breast milk in terms of fatty acid and triglyceride composition;

[0033] 2) This product first adds exogenous phospholipids to form a membrane solution, and then mixes it with other oils for homogenization, making the compound oil closer to breast milk in composition and structure;

[0034] 3) Compared with the compounded fats in conventional goat milk-based infant formula, the fat composition and structure of this product are similar to breast milk, which is more conducive to the fat digestion of infants. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0036] Figure 1 For ultra-high resolution microscopy.

[0037] Figure 2 Super-high resolution imaging of different digestion stages. DETAILED DESCRIPTION

[0038] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] A compound oil that simulates the lipid composition and structure of breast milk. According to laboratory preliminary experiments and relevant data collection, relevant data on the total fatty acid and triglyceride content in breast milk fat and oil raw materials are obtained. The preliminary experiments specifically include:

[0040] 1) Determination of total fatty acids: Dissolve the oil sample in n-hexane (30 mg / mL), add 2 mL of sulfuric acid-methanol solution (1%, v / v) after ultrasonic treatment for 3-5 min. Ultrasonic treatment was performed again, and the mixture was placed in a 60°C water bath for half an hour (shaking every 5 min), and 1 mL of n-hexane and 4 mL of ultrapure water were added. 1 mL of the upper organic phase was taken, and 0.5 g of sodium sulfate solid was added to absorb water, and the mixture was centrifuged at 4000 r / min for 5 min. After centrifugation, 200 μL of the supernatant was mixed with 4 mL of chromatographic grade n-hexane, and 40 μL of methyl salicylate (5.905 mg / mL) was added as an internal standard. The mixture was filtered through a 0.22 μm organic filter membrane and placed in a sample injection bottle for testing.

[0041] The chromatographic conditions were: Agilent DB-WAX capillary column (30m×0.25mm ID×0.25μm) gas chromatography system separation. Maintain at 50℃ for 3min, increase to 220℃ at a rate of 10℃ / min; maintain for 20min. Helium was used as carrier gas. Injection was 10μL, split ratio 30:1. Mass spectrometry conditions were: injection temperature 280℃; ion source temperature 230℃; transfer line temperature 250℃. Electron impact ionization (EI) source, SIM scanning mode, electron energy 70eV.

[0042] 2) Determination of triglycerides: Take the oil sample and dissolve it in chloroform-methanol (2:1, v / v) to make a 1 mg / mL solution, pass it through a 0.22 nm organic phase filter membrane and then perform UPLC-QTOF / MS detection. The triglycerides of all samples are identified by their positively charged quasi-separators [M+NH4]+ and identified based on diglyceride fragment ions. Since the UPLC-Triple-TOF-MS / MS used in this study cannot separate isomers, the fatty acids in the triglyceride structure in this article are randomly arranged and do not show position specificity.

[0043] The chromatographic conditions were as follows: Phenomen Kinetex 1.7u C18100A column; mobile phase A was water / methanol / acetonitrile (1:1:1, v / v / v) + 10mM ammonium acetate; mobile phase B was isopropanol / acetonitrile (1:1, v / v) + 10mM ammonium acetate. Gradient elution: 0-1.0min, 80% A; 1.0-6.0min, 80%-20% A; 6.0-10.0min, 20%-5% A; 10.0-12.0min, 5% A; 12.0min-20.0min, 5%-80% A. Flow rate 300μL / min, column temperature 35℃, injection 2μL. Mass spectrometry conditions were as follows: primary and secondary mass spectrometry data acquisition based on IDA function under the control of control software (Analyst TF 1.7, AB Sciex). In each data acquisition cycle, the secondary mass spectrometry data corresponding to the molecular ions with the strongest intensity greater than 100 are collected. The bombardment energy is 35 eV, and 15 secondary spectra are collected every 50 ms. The ESI ion source parameters are set as follows: the nebulizer pressure (GS1) is 60 Pa; the air curtain pressure is 0 Pa, the auxiliary gas pressure is 60 Pa; the temperature is 550 ° C; the spray voltage is 5500 V.

[0044] Data processing: Peakview software was used for peak recognition, peak extraction, peak integration, peak alignment, and fat identification and quantification.

[0045] The raw material scoring principles are as follows:

[0046] The scoring principle is the same as the crude oil physical mixing simulation conditions:

[0047] G=G1+G2

[0048]

[0049] Among them, G is the total score of 100 points, G1 and G2 are the total scores of conditions 1 (triglycerides) and 2 (total fatty acids), 50 and 50 points respectively.

[0050]

[0051] in, is the weight of breast milk triglyceride TAG / total fatty acids FAs; Di is the average value of breast milk TAG / FAs; Ci is the floating coefficient; Bi is the content value of breast milk TAG / FAs after mixing in different proportions; Ai is the range value of breast milk TAG;

[0052] When Bi>Ai maximum value, Ai takes the breast milk range Max; when Bi<Ai minimum value, Ai takes the breast milk range Min; when Bi is within the Ai range, Ci=0.

[0053] Example 1

[0054] A compound oil simulating the lipid composition and structure of breast milk, the preparation of which comprises the following steps:

[0055] 1) Based on laboratory preliminary experiments and relevant data collection, the relevant data on the total fatty acid and triglyceride content in breast milk fat and oil raw materials were obtained, and 8 major fatty acids and 6 major triglycerides with a content greater than 1% in breast milk were selected as templates to determine the corresponding total fatty acid and triglyceride contents in oil raw materials (Table 1, Table 2).

[0056] Table 1 Total fatty acid content of 8 kinds of raw materials

[0057]

[0058]

[0059] Table 2 Content of 6 kinds of triglycerides in each raw material

[0060]

[0061] 2) Computer simulation was performed using Matlab software to obtain different raw material ratios.

[0062] 3) Use the scoring principle to score the obtained oil raw materials. The higher the score, the closer it is to the composition of breast milk:

[0063] Table 3 Scoring and proportion of each raw material

[0064]

[0065]

[0066] 4) Select the raw material ratio of the group with the highest score as the formula for preparing the compound oil. Mix the weighed egg yolk lecithin and soybean lecithin, add purified water to make a 2% (W / V) solution, and stir magnetically overnight to completely dissolve it.

[0067] 5) Add the weighed vegetable oil, OPO structured oil and goat milk fat to the solution prepared in step 4, and high-speed shear for 10 minutes at 10000 r / min to obtain an emulsion.

[0068] 6) The emulsion obtained in step 5 is homogenized at 25 MPa for 3-5 times to obtain a composite oil simulating the lipid composition and structure of breast milk.

[0069] Verification experiment example:

[0070] In order to further verify the certainty of the effect of the formula of the present invention, the compounded oil of the embodiment of the present invention was compared with comparative examples 1 and 2 by ultra-high resolution microscope analysis.

[0071]

Comparative Example 1

[0072] [Comparative Example 2] Commercially available goat milk-based infant formula.

[0073] Experimental method: The emulsion sample (0.2 mL) was diluted to 10 mL with ultrapure water and gently mixed. The fluorescent probe Rd-DOPE was used to label the phospholipids at the lipid droplet interface at a concentration of 1 mg / mL (chloroform configuration). 1 mg / mL (Milli-Q configuration) of FCF labeled the protein concentration at the interface of the lipid droplets of the simulated fat globules. Next, 120 μL of the sample was mixed with 15 μL of Rd-DOPE and 60 μL of FCF and incubated in the dark at room temperature for 1 hour. The mixture (0.5 μL) was dropped on a glass slide, and the edge of the coverslip was painted with nail polish to fix the sample in place and prevent it from drying. The sample observation temperature was 25 ° C, and the excitation wavelengths corresponding to Rd-DOPE and FCF were 568 nm and 488 nm, respectively. The super-resolution microscope was equipped with a 63x oil lens. The numerical aperture of the lens was 1.42.

[0074] Depend on Figure 1 It can be seen that the ultra-high resolution microscope imaging of the embodiment is more similar to that of comparative example 1, both of which are phospholipid-protein complexes wrapped on the glyceride surface, while comparative example 2 shows a dense protein wrapping phenomenon.

[0075] Example 2

[0076] A compound oil simulating the lipid composition and structure of breast milk, the preparation of which comprises the following steps:

[0077] 1) Based on laboratory preliminary experiments and relevant data collection, relevant data on the total fatty acid and triglyceride content in breast milk fat and oil raw materials were obtained, and 8 major fatty acids and 6 major triglycerides with a content greater than 1% in breast milk were selected as templates to determine the corresponding total fatty acid and triglyceride contents in oil raw materials (Table 4, Table 5).

[0078] Table 4 Total fatty acid content of 8 kinds of raw materials

[0079]

[0080] Table 5 Triglyceride content of 6 kinds of raw materials

[0081]

[0082] 2) Computer simulation was performed using Matlab software to obtain different raw material ratios.

[0083] 3) Use the scoring principle to score the obtained oil raw materials. The higher the score, the closer it is to the composition of breast milk:

[0084] Table 6 Scoring and proportion of each raw material

[0085]

[0086]

[0087] 4) Select the raw material ratio of the group with the highest score as the formula for preparing the compound oil. The weighed egg yolk lecithin is prepared into a 2% (W / V) solution with purified water, and stirred magnetically overnight to completely dissolve it.

[0088] 5) Add the weighed vegetable oil, OPO structured oil and goat milk fat to the solution prepared in step 4, and high-speed shear for 10 minutes at 10000 r / min to obtain an emulsion.

[0089] 6) The emulsion obtained in step 5 is homogenized at 25 MPa for 3-5 times to obtain a composite oil simulating the lipid composition and structure of breast milk.

[0090] Example 3

[0091] A compound oil simulating the lipid composition and structure of breast milk, the preparation of which comprises the following steps:

[0092] 1) Based on laboratory preliminary experiments and relevant data collection, the relevant data on the total fatty acid and triglyceride content in breast milk fat and oil raw materials were obtained, and 8 major fatty acids and 6 major triglycerides with a content greater than 1% in breast milk were selected as templates to determine the corresponding total fatty acid and triglyceride contents in oil raw materials (Tables 7 and 8).

[0093] Table 7 Total fatty acid content of 8 kinds of raw materials

[0094]

[0095] Table 8 Triglyceride content of 6 kinds of raw materials

[0096]

[0097]

[0098] 2) Computer simulation was performed using Matlab software to obtain different raw material ratios.

[0099] 3) Use the scoring principle to score the obtained oil raw materials. The higher the score, the closer it is to the composition of breast milk:

[0100] Table 9 Scoring and proportion of each raw material

[0101]

[0102] 4) Select the raw material ratio of the group with the highest score as the formula for preparing the compound oil. Weigh the egg yolk lecithin and prepare it into a 2% (W / V) solution with purified water, and stir it with a magnetic stirrer overnight to completely dissolve it.

[0103] 5) Add the weighed vegetable oil, OPO structured oil and goat milk fat to the solution prepared in step 4, and high-speed shear for 10 minutes at 10000 r / min to obtain an emulsion.

[0104] 6) The emulsion obtained in step 5 is homogenized at 25 MPa for 3-5 times to obtain a composite oil simulating the lipid composition and structure of breast milk.

[0105] Example 4

[0106] A compound oil simulating the lipid composition and structure of breast milk, the preparation of which comprises the following steps:

[0107] 1) Based on laboratory preliminary experiments and relevant data collection, relevant data on the total fatty acid and triglyceride content in breast milk fat and oil raw materials were obtained, and 8 major fatty acids and 6 major triglycerides with a content greater than 1% in breast milk were selected as templates to determine the corresponding total fatty acid and triglyceride contents in oil raw materials (Tables 10 and 11).

[0108] Table 10 Total fatty acid content of 8 kinds of raw materials

[0109]

[0110]

[0111] Table 11 Triglyceride content of 6 kinds of raw materials

[0112]

[0113] 2) Computer simulation was performed using Matlab software to obtain different raw material ratios.

[0114] 3) Use the scoring principle to score the obtained oil raw materials. The higher the score, the closer it is to the composition of breast milk:

[0115] Table 12 Scoring and proportion of each raw material

[0116]

[0117] 4) Select the raw material ratio of the group with the highest score as the formula for preparing the compound oil. Mix the weighed egg yolk lecithin and soybean lecithin, add purified water to prepare a 2% (W / V) solution, and stir magnetically overnight to completely dissolve them.

[0118] 5) Add the weighed vegetable oil, OPO structured oil and goat milk fat to the solution prepared in step 4, and high-speed shear for 10 minutes at 10000 r / min to obtain an emulsion.

[0119] 6) The emulsion obtained in step 5 is homogenized at 25 MPa for 3-5 times to obtain a composite oil simulating the lipid composition and structure of breast milk.

[0120] In vitro digestion test

[0121] Test method: The parameters of the digestive enzymes in the static in vitro digestion model used in the present invention are shown in the following table.

[0122] Table 13 In vitro digestive enzyme activity

[0123]

[0124] Pepsin and lipase were dissolved in ultrapure water, pancreatin and lipase were dissolved in 1.25 times the concentration of intestinal phase stock solution, and bile salts were dissolved in 1.25 times the concentration of intestinal phase stock solution, and stored at 4°C. Gastric stock solution: NaCl 94mM, KCl 13mM, pH 5.3. Intestinal stock solution: NaCl 164mM, KCl 10mM, NaCO3 85 mM, CaCl2 3mM, pH 6.6.

[0125] Take 126 mL of milk sample, add 74 mL of gastric reserve solution, 17.866 mg of pepsin, 126.66 mg of gastric lipase, adjust the pH to 5.3, and incubate the digestion products after 30 minutes and 60 minutes at 37°C in a boiling water bath to inactivate the enzymes. Take 136 mL of gastric digestion fluid, add 64 mL of intestinal reserve solution, add 12.8 mg of trypsin, 600 mg of pancreatic lipase, and 925.37 mg of bile salt, adjust the pH to 6.6, and incubate the digestion products after 60 minutes and 120 minutes at 37°C in a boiling water bath to inactivate the enzymes.

[0126] Digested samples (0.2 mL) at different stages were diluted to 10 mL with ultrapure water and gently mixed. The triglycerides inside the lipid droplets were labeled with the fluorescent probe NR at a concentration of 42 μg / mL (ethanol configuration). 1 mg / mL (Milli-Q configuration) of FCF labeled the protein concentration at the interface of the lipid droplets of the simulated fat globules. Next, 100 μL of the sample was mixed with 20 μL NR and 50 μL FCF and incubated in the dark at room temperature for 1 hour. The mixture (0.5 μL) was dropped on a glass slide, and nail polish was applied to the edge of the coverslip to fix the sample in place and prevent it from drying. The sample observation temperature was 25 ° C, and the excitation wavelengths corresponding to NR and FCF were 639 nm and 488 nm, respectively. The super-resolution microscope was equipped with a 63x oil lens. The numerical aperture of the lens is 1.42.

[0127] Depend on Figure 2 It can be seen that the microstructure of the embodiment at different digestion stages is more similar to that of comparative example 1, and the spherical structure can be basically maintained during the digestion process.

[0128] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for preparing a compound oil simulating the lipid composition and structure of breast milk, characterized in that: The following steps are involved: (1) Using the main fatty acids and triglycerides with a content greater than 1% in breast milk fat as a template, the corresponding total fatty acid and triglyceride contents in oil raw materials and phospholipids were determined; (2) Computer simulation was performed using Matlab software to obtain different raw material ratios; (3) Using the scoring principle, score the oil raw materials and phospholipids with different ratios, and select the raw material ratio with the highest score; (4) preparing the phospholipid into a 2% (W / V) solution with purified water, and stirring magnetically overnight to completely dissolve it; (5) adding the oil raw material to the solution prepared in 4) and shearing to obtain an emulsion; (6) homogenizing the emulsion to obtain a compound oil that simulates the lipid composition and structure of breast milk.

2. The method for preparing a composite oil simulating the lipid composition and structure of breast milk according to claim 1, characterized in that: In step (1), the main fatty acids in the breast milk fat with a content greater than 1% include C10:0, C12:0, C14:0, C16:0, C16:1, C18:0, C18:1, and C18:

2.

3. The method for preparing a composite oil simulating the lipid composition and structure of breast milk according to claim 1, characterized in that: In step (1), the triglycerides with a content greater than 1% in the breast milk fat include OPL, OPO, OOO, PPL, PPO, and LLL.

4. The method for preparing a compound oil simulating the lipid composition and structure of breast milk according to claim 1, characterized in that: The oil raw materials in step (1) include vegetable oil, OPO structured oil and goat milk.

5. The method for preparing a compound oil simulating the lipid composition and structure of breast milk according to claim 4, characterized in that: The vegetable oil includes one or more of soybean oil, corn oil, sunflower oil, palm oil, coconut oil, linseed oil, walnut oil and rapeseed oil.

6. The method for preparing a compound oil simulating the lipid composition and structure of breast milk according to claim 1, characterized in that: In step (1), the phospholipids include one or more of egg yolk lecithin and soybean lecithin.

7. The method for preparing a compound oil simulating the lipid composition and structure of breast milk according to claim 1, characterized in that: In step (3), the scoring principle is: The scoring principle is the same as the crude oil physical mixing simulation conditions: G=G1+G2 Among them, G is the total score of 100 points, G1 and G2 are the total scores of conditions 1 (triglycerides) and 2 (total fatty acids), 50 and 50 points respectively. in, is the weight of breast milk triglyceride TAG / total fatty acids FAs; Di is the average value of breast milk TAG / FAs; Ci is the floating coefficient; Bi is the content value of breast milk TAG / FAs after mixing in different proportions; Ai is the range value of breast milk TAG; When Bi>Ai maximum value, Ai takes the breast milk range Max; when Bi<Ai minimum value, Ai takes the breast milk range Min; when Bi is within the Ai range, Ci=0.

8. The method for preparing a compound oil simulating the lipid composition and structure of breast milk according to claim 1, characterized in that: The shearing in step (5) is high-speed shearing at 10000 r / min for 10 min.

9. The method for preparing a compound oil simulating the lipid composition and structure of breast milk according to claim 1, characterized in that: The homogenization in step (6) is performed at 25 MPa for 3-5 times.

10. An application of a compound oil simulating the lipid composition and structure of breast milk, characterized in that: Use of the compound fat prepared according to any one of claims 1 to 9 in goat milk-based infant formula.

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