A skin care composition having a reinforced skin barrier function and uses thereof

Abstract

The application discloses a skin care composition with a skin barrier strengthening function and application thereof. The skin care composition is prepared by compounding persimmon leaf extract and / or coastal angelica extract on the basis of hyaluronic acid lysine, and the skin care composition has a good skin barrier strengthening effect, and can be used in cosmetics to improve skin barrier and redness and the like.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a skin care composition with enhanced skin barrier function and application thereof. BACKGROUND

[0002] It is believed that the occurrence of sensitive skin is a complex process involving skin barrier, neurovascular response and innate immune inflammation. When the skin barrier function is impaired, the permeability of the epidermis increases, and external chemical substances, antigenic substances and microorganisms are more likely to invade the skin, causing inflammatory response, and the protective effect on the dermal nerve and blood vessels is weakened, thereby promoting the occurrence of sensitive skin. The integrity of the stratum corneum is crucial to maintaining the skin barrier function, which is mainly related to mature keratinocytes and intercellular lipids. The stratum corneum is classically described as a "brick and mortar" structure, i.e. mature keratinocytes are flattened, de-nucleated and surrounded by a cornified envelope (CE) to form the so-called "brick". The assembly of the cornified envelope (CE) begins from the upper layer of the stratum spinosum, mainly composed of filaggrin (FLG), involucrin (IVL), loricrin (LOR), keratin (KRT) and late cornified envelope proteins (LCEs). The hydrophobic lipid matrix seals the mature stratum corneum cells in place as the "mortar" of this physical barrier. The lipids that make up this matrix are composed of additional ceramides, fatty acids and cholesterol, which are related to ceramides in the lipid envelope of the cornified envelope (CE).

[0003] Abnormal organization and composition of the lipid matrix lead to an increase in TEWL (trans-epidermal water loss) and allergen penetration. Filaggrin monomers (FLG) are cleaved into their constituent amino acids on the surface of the SC. These amino acids form a hygroscopic natural moisturizing factor (NMF) that maintains an acidic pH and protects against UVB rays. The reduction of FLG is further associated with skin barrier dysfunction by reducing water retention and increasing TEWL values. IVL is mainly expressed on the upper part of the stratum spinosum and the granular layer, and is a marker protein for keratinocyte differentiation, located on the outer layer of the CE, covalently bound to ceramides containing -OH, thereby connecting the lipid matrix and keratinocytes to play a role. LOR is mainly expressed in the granular layer and stratum corneum, and is the main constituent of the finally differentiated CE, accounting for about 80% of its protein amount, and plays a key role in reinforcement. Released FLG monomers are degraded into free amino acids, including glutamine, arginine and histidine, and then converted into urocanic acid (UCA) and pyrrolidine carboxylic acid (PCA). This process is mediated by other protease groups, including caspase 14, calpain 1 and bleomycin hydrolase. PCA is the main component of natural moisturizing factor (NMF), which is responsible for maintaining moisture in the stratum corneum, thereby enhancing barrier function.

[0004] Lysine hyaluronate (LH) is an organic salt compound combined by hyaluronic acid and L-lysine through ionic bonds. Since HA combines lysine with moisturizing, antioxidant and anti-allergic effects, it has good moisturizing effect and certain antioxidant effect, and has certain anti-aging and whitening effect when applied to skin care products. LH has been used in the field of cosmetics for more than ten years, and is mostly used as a moisturizing agent in skin care products due to its weak antioxidant effect. It is disclosed in CN202311532988.1 that Lysine hyaluronate has an inhibitory effect on free radicals induced by ultraviolet radiation, so it has antioxidant and anti-aging functions. At present, there are few studies on Lysine hyaluronate in the direction of barrier repair. SUMMARY

[0005] In view of the above problems, the present application provides a skin care composition with strong skin barrier function and its application. The present application combines persimmon leaf extract and / or coastal angelica extract on the basis of lysine hyaluronate. The test proves that it has good effect of strengthening skin barrier and can be used in cosmetics to improve skin barrier and redness problems.

[0006] The technical solution of the present application is: a skin care composition with strong skin barrier function, characterized in that it comprises the following components by mass: 0.1-1.5 parts of lysine hyaluronate, 1.0-15.0 parts of persimmon leaf extract and / or coastal angelica extract.

[0007] Preferred ratio: 0.1-1.0 parts of lysine hyaluronate, 1.0-10.0 parts of persimmon leaf extract, and 1.0-10.0 parts of coastal angelica extract.

[0008] The lysine hyaluronate (LHA) is the same as the patent 202311532988.1, which is an organic salt combined by hyaluronic acid and L-lysine through ionic bonds. The number of L-lysine combined with each disaccharide unit molecule of hyaluronic acid is 0.4-1, i.e. the content of L-lysine in LHA is 10-30% (g / g), preferably the content of L-lysine is 13-19% (g / g). The average molecular weight of the above-mentioned lysine hyaluronate is 7kDa-1000kDa, preferably the average molecular weight is 10kDa-600kDa, more preferably the average molecular weight is 100kDa-300kDa. The preparation method is as follows: put sodium hyaluronate into a filtered and sterilized lysine hydrochloride solution, stir and dissolve, and react for 1-2 hours, then add ethanol for precipitation, filter and wash the precipitate with ethanol for purification, and finally dry under reduced pressure to obtain the finished product.

[0009] The persimmon leaf extract can be obtained by a commercial method or a self-made method, preferably by ultrasonic extraction at 50-60 DEG C using a deep eutectic solvent (DES) (hydrogen bond acceptor (HBA) is aspartic acid, hydrogen bond donor (HBD) is glycerol, and water is added for preparation), and the persimmon leaf extract is obtained by filtering the concentrated extract under reduced pressure. The concentration of the persimmon leaf extract is 0.08-0.13 g / mL (calculated by the amount of crude drug), that is, 0.08-0.13 g of dried persimmon leaf powder is used to obtain 1 mL of persimmon leaf extract (solution).

[0010] The coastal angelica extract can be obtained by a commercial method or a self-made method, preferably by heating reflux extraction using 60%-75% ethanol solution, and the concentrated extract is obtained by filtering the concentrated extract after being concentrated under reduced pressure and evaporated to be free of obvious ethanol smell, and then 1.0-2.0 times the mass of butanediol is added and stirred uniformly.

[0011] Preparation method: the hyaluronic acid lysine, the persimmon leaf extract and / or the coastal angelica extract are mixed together and dissolved in purified water (supplemented to 100 parts).

[0012] The persimmon leaf extract has the effects of antioxidation, anti-inflammation, blood lipid reduction, antibiosis, antiviral and skin whitening. The coastal angelica belongs to the perennial herbaceous plant of the Umbelliferae family, is a rare plant for both medicine and food, has the effects of improving body immunity, moistening the intestines and defecation, improving sleep, anti-aging and antioxidation. The above-mentioned raw materials have synergistic effects, and the test proves that the above-mentioned composition has good effects of strengthening the skin barrier and can be used in cosmetics to improve the skin barrier and redness.

[0013] The application also provides the use of the above-mentioned composition in the preparation of cosmetics with the function of strengthening the skin barrier. The test proves that the above-mentioned composition can promote the expression of the related proteins (FLG, LOR, IVL, Caspase14, AQP3 expression), enzymes and ceramides, etc. in the formation of the skin barrier, so as to effectively strengthen the skin barrier.

[0014] The application also provides a cosmetic with the function of strengthening the skin barrier, characterized in that the cosmetic contains 0.5-30% (preferably 2-20%) of the skin care composition with the function of strengthening the skin barrier.

[0015] The above-mentioned skin care cosmetic with the function of strengthening the skin barrier can also contain emulsifiers, emollients, humectants, thickening agents, preservatives, pH value adjusting agents and other raw material ingredients allowed to be added in the cosmetic field.

[0016] Meanwhile, the present application can also prepare different cosmetic dosage forms such as essence water, essence liquid, facial mask, essence milk, essence cream, etc. by reasonably adding the above raw material components, and further derive cosmetic categories based on the above basic cosmetic categories, which are obviously within the protection scope of the present application.

[0017] The beneficial technical effects of the present application are:

[0018] 1. The in vitro skin barrier function enhancement test proves that the hyaluronic acid lysine of the present application has a synergistic effect with one or both of the persimmon leaf extract and the coastal angelica extract, can promote the expression of filaggrin (FLG), involucrin (IVL), loricrin (LOR), ceramide, cysteine aspartate protease 14 (Caspase 14) and aquaporin (AQP3), and achieve the effect of strengthening the skin barrier.

[0019] 2. The hyaluronic acid lysine has a weak promoting effect on the synthesis of FLG, LOR, IVL and other proteins, and has no effect on the synthesis of ceramide. The combination of the hyaluronic acid lysine, the persimmon leaf extract and the coastal angelica extract can more significantly promote the expression of FLG, LOR, IVL, ceramide, Caspase 14 and AQP3 proteins, and expand the function and application of the hyaluronic acid lysine in repairing the skin barrier.

[0020] 3. When the hyaluronic acid lysine is combined with the persimmon leaf extract and / or the coastal angelica extract, the color change problem of the persimmon leaf extract and the coastal angelica extract in the water agent formula can be changed, and the stability of the formula can be greatly improved.

[0021] 4. The human efficacy experiment test proves that the skin care composition containing the skin barrier strengthening composition has good repairing effect (after using the cosmetic containing 7.0% skin barrier strengthening composition for 4 weeks, the a* value of the red area analysis is significantly reduced, and the skin TEWL is significantly reduced). The preparation method of the cosmetic of the present application is simple and easy to operate, the raw materials are easy to obtain, and is suitable for mass production, so it has good practical application value. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 Figure 6 is a diagram showing the changes in the facial red area of the 15th subject of Example 6 before and after using the sample (D0, D14 and D28); DETAILED DESCRIPTION

[0023] The application is further illustrated by the following examples, but the application is not limited to the scope of the examples. Based on the examples in the application, any changes to the application without creative labor are within the protection scope of the application. In the examples of the application, all raw materials for preparation are commercially available products known to those skilled in the art, unless otherwise specified.

[0024] Examples 1-5:

[0025] The skin care composition with the skin barrier strengthening effect of the application is composed of hyaluronic acid lysine, persimmon leaf extract and / or coastal angelica extract. The proportions of Examples 1-5 and Comparative Examples 1-4 are shown in Table 1 below, calculated by mass ratio.

[0026] Preparation method: mix hyaluronic acid lysine, persimmon leaf extract and / or coastal angelica extract (according to the ingredients and amounts in Table 1) together and dissolve in purified water.

[0027] Table 1: Ingredient list of Examples 1-5 and Comparative Examples 1-4 (mass ratio)

[0028]

[0029] The hyaluronic acid lysine used in the examples of the application is the same as Composition 1-4 in Table 2 of Patent 202311532988.1 (average molecular weight 720 kDa, L-lysine content 19.6%).

[0030] The preparation method of persimmon leaf extract is as follows: dry and pulverize persimmon leaves through a 60-mesh sieve, and store in a dry and cool place at room temperature. The eutectic solvent DES is prepared as follows: the hydrogen bond acceptor (HBA) is aspartic acid, and the hydrogen bond donor (HBD) is glycerol. Mix HBA and HBD in a 1:2 molar ratio, and stir in a water bath at 80°C and 1000 r / min until a uniform transparent liquid is formed. Add 20% (V / V) distilled water to it and stir to obtain the eutectic solvent. Weigh an appropriate amount of dried persimmon leaf powder, add DES, and the solid-liquid ratio is 1:10. Ultrasonic extraction is carried out at 50°C for 30 min, with an ultrasonic power of 300 W and an ultrasonic frequency of 40 KHz. Repeat the extraction once, combine the two extraction liquids, and filter. The filtrate is concentrated under reduced pressure at 55°C, and the persimmon leaf extract is obtained after filtration. The concentration of persimmon leaf extract is 0.1150 g / mL (calculated by the amount of crude drug).

[0031] The method for obtaining the extract of Angelica sinensis from Binhai is as follows: Dry Angelica sinensis is pulverized and passed through a 150-mesh sieve. An appropriate amount of the dried Angelica sinensis powder is placed in a reflux jar, and 65% ethanol solution is added at a mass ratio of 1:7. The mixture is heated and refluxed at 65°C for 1 hour. This extraction is repeated three times. The filtrates are combined, and the filtrate is concentrated under reduced pressure until no obvious ethanol odor is detected. Butylene glycol, at a mass ratio of 1.5 times that of the concentrate, is added, stirred evenly, and filtered to obtain the Angelica sinensis extract. The concentration, based on the crude drug, is 0.4 g / mL, meaning 0.4 g of dried Angelica sinensis powder yields 1 mL of Angelica sinensis extract (solution).

[0032] Example 6: Essence Water

[0033] The above-mentioned skin care composition with enhanced skin barrier function is used to prepare an essence water product containing the composition with enhanced skin barrier function, and the formula is shown in Table 2.

[0034] Table 2: Formula of Essence Water Products

[0035]

[0036] Preparation method of essence water:

[0037] S1: Add the raw materials from phase A separately, heat to 85℃ and stir to dissolve. After complete dissolution, start cooling.

[0038] S2: When the temperature drops to 45℃, add phase B and phase C components in sequence, stir to dissolve and disperse evenly, and the essence water product is obtained.

[0039] The preparation methods of Examples 7 and 8, and Comparative Examples 5 to 8 are the same as those of Example 6, except that Examples 7 and 8, and Comparative Examples 5 to 8 do not contain the skin-barrier-strengthening skin care composition of Example 2, the B phase of Examples 7 and 8 is 7.0% of Examples 4 and 5, and the B phase of Comparative Examples 5 to 8 is 7.0% of Comparative Examples 1 to 4.

[0040] Experiment 1: In vitro promotion of barrier-related protein expression FLG, LOR, IVL, Caspase14, AQP3 and ceramide synthesis

[0041] use 3D skin models were used for experiments. According to the test protocol in Table 3, the models were transferred to 6-well plates, with three replicates per group. For groups BC and NC, 0.9 mL of EpiGrowth medium (Boxi Biotechnology) was added to each well, while for group PC, 0.9 mL of medium containing WY14643 (Sigma) was added to each well. The test group numbers were labeled on the 6-well plates. The working solution for the sample group was added to the model surface. UVB irradiation (irradiation dose of 600 mJ / cm²) was then applied to the groups requiring UVB irradiation according to the test protocol (Table 3). 2After irradiation, the model was placed in a CO2 incubator (37°C, 5% CO2) for continued incubation for 24 h. After incubation, the surface of the model was washed with sterile PBS to remove residual test substance, and the residual liquid inside and outside the model was wiped off with a sterile cotton swab. The model for detection was cut around and removed, and was fixed with 4% paraformaldehyde. After 24 h of fixation, immunofluorescence or immunohistochemical detection of barrier formation-related proteins (FLG, LOR, IVL) and AQP3 and Caspase14 related to barrier function was performed, and the pictures were observed under a microscope, collected, and analyzed.

[0042] Ceramide test: lipid sample extraction - the model was placed in a centrifuge tube, and proteinase K solution (Thermo, 17916) was added for 50°C water bath for 2 h. The viscous material on the horny layer was peeled off with tweezers, and the water was absorbed, and then the horny layer was placed in a glass tube. 1 mL of chloroform-methanol mixture (volume ratio of chloroform to methanol is 1:1) was added to each glass tube, and ice bath ultrasonic was performed. The supernatant was taken to the sample bottle, dried, and ceramide detection - acetonitrile: isopropanol (1:1) mixed solution was added to the nitrogen-dried sample bottle, centrifuged at 12000 rpm for 10 min, and 100 μL of the upper layer was taken to the inner liner tube, and LC-MS was used for ceramide detection.

[0043] GraphPad Prism was used for plotting, and the results were expressed as Mean ± SD. t-test statistical analysis was used for comparison between groups. The statistical analysis was two-tailed. 0.01 < P < 0.05 was considered to have significant difference, and P < 0.01 was considered to have extremely significant difference.

[0044] Table 3: Test scheme

[0045]

[0046] The dilution of the sample group was performed by dilution with water.

[0047] Table 4: Summary table of FLG content detection results

[0048]

[0049]

[0050] (Note: ## indicates p < 0.01 compared with the blank control; ** indicates p < 0.01 compared with the negative control; * indicates 0.01 < p < 0.05 compared with the negative control.)

[0051] As can be seen from Table 4, Comparative Example 2 does not have the ability to promote the expression of FLG protein, indicating that single persimmon leaf extract does not have the effect of promoting the expression of FLG protein, and Examples 4 and 5 have synergistic promotion effect after compounding hyaluronic acid lysine with persimmon leaf extract and coastal angelica extract, respectively. Examples 2-3 have synergistic effect after mixing hyaluronic acid lysine, persimmon leaf extract and coastal angelica extract, and the effect of promoting the expression of FLG protein is significantly higher than that of other groups (Example 1 is lower than Examples 2-3 in the ability to promote the expression of FLG protein due to low content of effective components, but is significantly better than Examples 4, 5 and Comparative Examples 1-4), indicating that the three have significant synergistic promotion effect in promoting the expression of FLG protein.

[0052] Table 5: LOR content detection result summary table

[0053] Sample Name Relative IOD Average SD P-value Upregulation Rate (vs NC) BC 1.00 0.11 / / NC 0.36 0.04 0.000## / PC 0.99 0.12 0.001** 175.00％ Example 1 0.67 0.1 0.008** 86.11％ Example 2 0.96 0.11 0.001** 166.67％ Example 3 0.92 0.12 0.002** 155.56％ Example 4 0.60 0.09 0.013* 66.67％ Example 5 0.56 0.1 0.032* 55.56％ Comparative Example 1 0.52 0.11 0.077 44.44％ Comparative Example 2 0.41 0.12 0.531 13.89％ Comparative Example 3 0.35 0.1 0.880 -2.78％ Comparative Example 4 0.46 0.12 0.243 27.78％

[0054] (Note: ## indicates p<0.01 compared with the blank control; ** indicates p<0.01 compared with the negative control; * indicates 0.01<p<0.05 compared with the negative control.)

[0055] As can be seen from Table 5, except for Comparative Example 3, the rest of the groups have the function of promoting the expression of LOR protein, and Examples 2-3 have significantly higher effect of promoting the expression of LOR protein than other groups (Example 1 is lower than Examples 2-3 in the ability to promote the expression of LOR protein due to low content of effective components, but is significantly better than Examples 4, 5 and Comparative Examples 1-4), indicating that the three have significant synergistic promotion effect in promoting the expression of LOR protein.

[0056] Table 6: IVL content detection result summary table

[0057] Sample Name Relative IOD Average SD P-value Upregulation Rate (vs NC) BC 1.00 0.11 / / NC 0.42 0.05 0.000## / PC 1.53 0.12 0.0008** 264.29％ Example 1 0.83 0.1 0.003** 97.62％ Example 2 1.4 0.11 0.000** 233.33％ Example 3 1.26 0.13 0.000** 200.00％ Example 4 0.71 0.12 0.018* 69.05％ Example 5 0.74 0.1 0.008** 76.19％ Comparative Example 1 0.47 0.07 0.371 11.90％ Comparative Example 2 0.58 0.08 0.042* 38.10％ Comparative Example 3 0.53 0.08 0.114 26.19％ Comparative Example 4 0.66 0.08 0.012* 57.14％

[0058] (Note: ## indicates p<0.01 compared with the blank control; ** indicates p<0.01 compared with the negative control; * indicates 0.01<p<0.05 compared with the negative control.)

[0059] As can be seen from Table 6, the single hyaluronan lysine in Comparative Example 1 does not have obvious effect on promoting IVL protein expression, but has certain promoting effect after being compounded with persimmon leaf extract and Binhaigengcai extract, i.e. Example 4 and Example 5. The synergistic effect of the mixture of hyaluronan lysine, persimmon leaf extract and Binhaigengcai extract in Examples 2-3 on promoting IVL protein expression is significantly higher than that of other groups (Example 1 has lower content of effective components, and the ability of promoting IVL protein expression is not as good as that of Examples 2-3, but is obviously better than that of Examples 4, 5 and Comparative Examples 1-4), which indicates that the three components have significant synergistic effect on promoting IVL protein expression.

[0060] Table 7: Summary table of ceramide total concentration detection results

[0061] Sample Name Ceramide Total Concentration (pg / mL) SD P-value Upregulation Rate (vs NC) BC 47.59 7.21 / / NC 33.54 2.12 0.000## / PC 56.56 0.65 0.000** 68.63％ Example 1 44.81 0.63 0.001** 33.60％ Example 2 53.56 1.23 0.000** 59.69％ Example 3 50.87 1.06 0.000** 51.67％ Example 4 34.84 0.71 0.371 3.88％ Example 5 40.84 0.62 0.005** 21.77％ Comparative Example 1 32.60 0.74 0.509 -2.80％ Comparative Example 2 31.48 0.69 0.185 -6.14％ Comparative Example 3 38.84 0.61 0.014* 15.80％ Comparative Example 4 38.56 0.67 0.017* 14.97％

[0062] (Note: ## represents p<0.01 compared with the blank control; ** represents p<0.01 compared with the negative control; * represents 0.01<p<0.05 compared with the negative control.)

[0063] As can be seen from Table 7, Comparative Example 1 and Comparative Example 2 do not have the ability of promoting ceramide synthesis, which indicates that single hyaluronan lysine or persimmon leaf extract does not have the ability of promoting ceramide synthesis. However, there is synergistic effect between hyaluronan lysine and persimmon leaf extract after being mixed (Example 4), but the effect is not obvious. There is good synergistic effect of hyaluronan lysine and Binhaigengcai extract on promoting ceramide synthesis after being mixed (Example 5), but the synergistic effect of the mixture of hyaluronan lysine, persimmon leaf extract and Binhaigengcai extract in Examples 2-3 on promoting ceramide synthesis is significantly higher than that of other groups (Example 1 has lower content of effective components, and the ability of promoting ceramide synthesis is not as good as that of Examples 2-3, but is obviously better than that of Examples 4, 5 and Comparative Examples 1-4), which indicates that the three components have significant synergistic effect on promoting ceramide synthesis.

[0064] Table 8: Summary table of AQP3 content detection results

[0065]

[0066]

[0067] (Note: ## represents p<0.01 compared with the blank control; ** represents p<0.01 compared with the negative control; * represents 0.01<p<0.05 compared with the negative control.)

[0068] As can be seen from Table 8, Comparative Example 2 does not have the ability to promote AQP3 expression, indicating that single persimmon leaf extract does not have the ability to promote AQP3 expression, and Examples 4 and 5 have synergistic promotion after compounding hyaluronic acid lysine with persimmon leaf extract and coastal angelica extract, respectively. The synergistic promotion of AQP3 expression of Examples 2-3 after mixing hyaluronic acid lysine, persimmon leaf extract and coastal angelica extract is significantly higher than that of other groups (Example 1 has the same ability to promote AQP3 expression as Example 5 due to low effective ingredient content, but is significantly better than Examples 4 and Comparative Examples 1-4), indicating that the three have significant synergistic promotion in promoting AQP3 expression.

[0069] Table 9: Summary of Caspase 14 content detection results

[0070] Sample Name Relative IOD Average SD P-value Upregulation Rate (vs BC) BC 1.00 0.11 / / NC 0.25 0.02 0.000## / PC 0.70 0.09 0.001** 180.00％ Example 1 0.51 0.08 0.005** 104.00％ Example 2 0.77 0.1 0.001** 208.00％ Example 3 0.72 0.12 0.003** 188.00％ Example 4 0.49 0.08 0.007** 96.00％ Example 5 0.43 0.07 0.013* 72.00％ Comparative Example 1 0.42 0.06 0.010* 68.00％ Comparative Example 2 0.32 0.05 0.088 28.00％ Comparative Example 3 0.24 0.05 0.763 -4.00％ Comparative Example 4 0.33 0.08 0.130 32.00％

[0071] (Note: ## indicates p < 0.01 compared with the blank control; ** indicates p < 0.01 compared with the negative control; * indicates 0.01 < p < 0.05 compared with the negative control.)

[0072] As can be seen from Table 9, Comparative Example 3 does not have the ability to promote Caspase 14 expression, indicating that single coastal angelica extract does not have the ability to promote Caspase 14 expression, and Examples 4 and 5 have synergistic promotion after compounding hyaluronic acid lysine with persimmon leaf extract and coastal angelica extract, respectively. The synergistic promotion of Caspase 14 expression of Examples 2-3 after mixing hyaluronic acid lysine, persimmon leaf extract and coastal angelica extract is significantly higher than that of other groups (Example 1 has lower ability to promote Caspase 14 expression than Examples 2-3 due to low effective ingredient content, but is better than Examples 4 and 5 and Comparative Examples 1-4), indicating that the three have significant synergistic promotion in promoting Caspase 14 expression.

[0073] Test Example 2: Stability test of serum water system

[0074] YS6060H type table spectrophotometer was used to test Examples 6-8 and Comparative Examples 5-8, and whether there was a difference in the color of the sample preparation after 1 month of cold and hot cycle (-18℃, 4℃, 45℃, cycle once every 3 days), 45℃, 4℃, -18℃, and 3 months of light.

[0075] The test mode of the colorimeter was SCI, the light source was D65, the color space was CIE Lab, the observer angle was 10°, and the total tolerance of the standard sample △E (formula: △E = SQRT(△L 2 +△a 2 +△b 2The samples of Examples 6-8 and Comparative Examples 5-8 were cooled to room temperature after preparation and used as standards, and the samples were poured into cuvettes and placed in a transmission cell for testing. The L*, a* and b* values were tested, and the standard sample data were stored. The samples of Examples 6-8 and Comparative Examples 5-8 were placed in a cold-heat cycle (-18℃, 4℃, 45℃, cycled once every 3 days) for one month, and were placed in a light cycle (45℃, 4℃, -18℃, light) for 3 months. The colorimetric values L*, a* and b* were tested, and were compared with the standard sample, respectively. If ΔE>1, it was unqualified, and if ΔE≤1, it was qualified.

[0076] Table 10: Colorimetric value result summary table

[0077]

[0078] As shown in Table 10, in the aqueous system, the persimmon leaf extract alone is prone to color change under high temperature and light, the Binhaigengyu extract alone is prone to color change under high temperature, and only the combination of persimmon leaf extract and Binhaigengyu extract is prone to color change under high temperature and light. The combination of hyaluronic acid lysine and persimmon leaf extract, hyaluronic acid lysine and Binhaigengyu extract, and hyaluronic acid lysine, persimmon leaf extract and Binhaigengyu extract can change the color change phenomenon and greatly improve the appearance stability of the formula.

[0079] Test Example 3: Test results of cosmetics containing the skin care composition of the present application on human body repair

[0080] The serum of Example 6 was subjected to human skin repair efficacy test. A total of 33 people, Chinese healthy men and women, 4 men and 29 women, aged 18-45, met the subject volunteer selection and exclusion criteria.

[0081] Method: After cleansing and using cosmetic water in the morning and evening, an appropriate amount of serum was evenly applied to the face, gently massaged and absorbed, twice a day, morning and evening, for 28 consecutive days.

[0082] Detection time: before using the sample (D0), 14 days after using the sample (D14), and 28 days after using the sample (D28).

[0083] Test method and test parameters:

[0084] 1. Facial imaging test (VISIA-CR)

[0085] The facial image analyzer VISIA-CR (Canfield, USA) can provide safe, stable standard light conditions, all camera settings and imaging parameter settings are controlled by software to ensure the stability and standardization of imaging. The instrument has multiple light source modes: standard light, blue light, orange light, cross-polarized light, parallel polarized light, and can image the facial skin at multiple angles, with an imaging quality of up to 21 million pixels. Image analysis is performed on the red area picture to obtain the quantitative index red area analysis a* value, which is used to evaluate the improvement of facial skin redness and sensitive state.

[0086] 2. Skin moisture content test (Corneometer)

[0087] The skin moisture content tester Corneometer (CM 825, Courage and Khazaka, Germany) is based on the principle of capacitance to test the skin moisture content, which is used to detect the relative water content on the skin surface, the larger the value, the higher the water content.

[0088] 3. Trans-epidermal water loss TEWL test (Aqua Flux)

[0089] Used to detect the trans-epidermal water loss TWEL value of the skin, to evaluate the skin barrier function. The smaller the value, the better the water retention ability.

[0090] The test results are shown in Tables 11-13.

[0091] Table 11: Descriptive statistical results of red area analysis a* value (n=33)

[0092]

[0093]

[0094] Statistical analysis results: "-" : no statistically significant difference (P≥0.05); "*" : statistically significant difference (0.01≤P<0.05); "**" : statistically significant difference (0.001≤P<0.01); "***" : statistically significant difference (P<0.001).

[0095] Table 12: Descriptive statistical results of skin moisture content (n=33)

[0096]

[0097] Statistical analysis results: "-" : no statistically significant difference (P≥0.05); "*" : statistically significant difference (0.01≤P<0.05); "**" : statistically significant difference (0.001≤P<0.01); "***" : statistically significant difference (P<0.001).

[0098] Table 13: Descriptive statistics of TEWL values of trans-epidermal water loss (n=33)

[0099]

[0100] Statistical analysis results: "": no significant difference (P≥0.05); "": significant difference (0.01≤P<0.05); "": significant difference (0.001≤P<0.01); "": significant difference (P<0.001).

[0101] From Tables 11-13, it can be seen that the skin care composition containing the skin barrier function enhancing agent of Example 6 has good moisturizing effect and repairing effect on skin barrier function, and thus has good repairing effect on skin barrier function.

[0102] The facial red area change chart of the 15th subject of Example 6 before and after using the sample (D0, D14 and D28) is shown in Figure 6. Figure 1 As can be seen from the chart, the skin care composition containing the skin barrier function enhancing agent has good repairing effect on skin barrier function.

[0103] It should be noted that the above examples are only used to illustrate the technical solutions of the present application but not to limit the present application. Although the present application is explained in detail with reference to the examples, the technical solutions of the present application can be modified or replaced equivalently according to the needs without departing from the spirit and scope of the present application.

Claims

1. A skin care composition having enhanced skin barrier function, characterized in that, The composition comprises the following components in parts by mass: hyaluronic acid lysine 0.1-1.0 parts, persimmon leaf extract 1.0-10.0 parts, and Binha angelica extract 1.0-10.0 parts; The hyaluronic acid lysine is an organic salt of hyaluronic acid and L-lysine combined by ionic bonds, wherein the number of L-lysine combined with each disaccharide unit molecule of hyaluronic acid is 0.4-1, and the average molecular weight of the hyaluronic acid lysine ranges from 7 kDa to 1000 kDa; The persimmon leaf extract is prepared by ultrasonic extraction at 50-60 ℃ using a eutectic solvent, and the extract is concentrated under reduced pressure and filtered to obtain the persimmon leaf extract; the concentration of the persimmon leaf extract is 0.08-0.13 g / mL in terms of crude drug amount; and the eutectic solvent is prepared by adding water to a hydrogen bond acceptor aspartic acid and a hydrogen bond donor glycerol; The Binha angelica extract is prepared by heating reflux extraction using 60%-75% ethanol solution, and the extract is concentrated under reduced pressure and volatilized to no obvious ethanol smell, 1.0-2.0 times the mass of butanediol is added to the concentrated solution, stirred uniformly, filtered, and the Binha angelica extract is obtained; wherein the concentration is 0.35-0.50 g / mL in terms of crude drug amount.

2. The skin care composition of claim 1 wherein, The hyaluronic acid lysine, the persimmon leaf extract and / or the Binha angelica extract are mixed together and dissolved in purified water.

3. Use of the skin care composition of claim 1 or 2 in the preparation of a cosmetic product having the function of strengthening skin barrier.

4. The use according to claim 3, wherein the compound is ###0002### The skin barrier is effectively strengthened by promoting the expression of proteins, enzymes and ceramides related to the formation of the skin barrier.

5. A cosmetic having a function of strengthening skin barrier, characterized by comprising the composition according to any one of claims 1 to 4. The cosmetic product contains 0.5-30% of the skin care composition having the function of strengthening skin barrier of claim 2.

6. The cosmetic having a function of strengthening skin barrier as claimed in claim 5, wherein the cosmetic is a cosmetic for skin care. The cosmetic product is any one of essence, serum, mask, essence cream and essence cream. The cosmetic product is any one of essence, serum, mask, essence cream and essence cream.

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