Pesticide composition containing ningnanmycin and lentinan and application thereof

Abstract

The invention discloses a pesticide composition containing ningnanmycin and lentinan and application of the pesticide composition, and belongs to the technical field of crop planting, the composition comprises ningnanmycin, lentinan and glucosan, and the weight ratio of ningnanmycin to lentinan to glucosan in the composition is 1: (0.1-20): (0.1-30). The pesticide composition is compounded with the green tea component, so that the control effect can be further improved, and the pesticide composition is used for preventing and treating virus, bacteria and fungal diseases of crops, is safe and green to use, has small influence on the environment, can reduce pesticide residues in the crops, and has important significance on crop planting.

Description

Technical Field

[0001] The invention belongs to the technical field of crop planting, and in particular relates to a pesticide composition containing ningnanmycin and lentinan and application thereof. Background Art

[0002] Plant viral diseases are infectious diseases and the second largest plant disease. Their widespread prevalence seriously endangers the health of crops and can lead to significant reductions in the yields of food and cash crops such as wheat, rice, tobacco, and potatoes worldwide, causing huge economic losses.

[0003] At present, a large number of single-component chemical pesticides are often used for the prevention and control of crop viral diseases, such as guanidine hydrochloride and copper acetate, which can easily cause crops to develop resistance, resulting in reduced efficacy and inability to eliminate diseases; or chemical pesticides are combined with biological pesticides, such as the combination of dextran and cincomycin acetate. The existing technology not only has high manpower and material costs and a long prevention and control cycle, but also is difficult to effectively prevent and control a variety of crop diseases, and is accompanied by problems such as increased viral resistance and soil and water pollution.

[0004] How to combine and transform existing antiviral pesticides to improve their efficacy while reducing the environmental pollution caused during their use is of great significance to current environmental safety and food safety. Summary of the invention

[0005] In view of the above problems, the present invention discloses a pesticide composition containing Ningnanmycin and Lentinan and application thereof.

[0006] The present invention adopts the following technical solution:

[0007] A pesticide composition containing ningnanmycin and lentinan, the composition comprising ningnanmycin, lentinan and glucan, the weight ratio of ningnanmycin, lentinan and glucan in the composition is 1:(0.1-20):(0.1-30), and the composition is used for preventing and controlling crop viral diseases.

[0008] Furthermore, the weight ratio of Ningnanmycin, Lentinan and glucan in the composition is 1:(0.2-0.5):(4-6), and the crops include tobacco, rice or potato.

[0009] Furthermore, the weight ratio of Ningnanmycin, Lentinan and glucan in the composition is 1:0.3:5.

[0010] Furthermore, the composition also includes a green tea component, and the method for obtaining the green tea component includes: extracting green broken tea with a 60% ethanol solution to obtain an extract, the extraction temperature is 80-85°C, the weight ratio of the ethanol solution to the green broken tea is (15-18):1, evaporating the extract until there is no ethanol taste to obtain an ethanol-free water extract, adding an equal volume of ethyl acetate for extraction, extracting twice continuously, concentrating and drying the aqueous phase after the two ethyl acetate extractions to obtain the green tea component.

[0011] Furthermore, the weight ratio of Ningnanmycin, Lentinan, glucan and green tea components in the composition is 1:0.3:5:(0.5-3).

[0012] Furthermore, the weight ratio of Ningnanmycin, Lentinan, glucan and green tea components in the composition is 1:0.3:5:1.5.

[0013] Furthermore, the composition also includes brown sugar, and the weight ratio of ningnanmycin, lentinan, glucan, green tea components and brown sugar in the composition is 1:0.3:5:1.5:(0.1-0.2). The brown sugar in the present invention is a finished sugarcane sugar made from sugarcane as raw material, through juice extraction, clarification, boiling, and lime method.

[0014] Furthermore, the weight ratio of Ningnanmycin, Lentinan, glucan, green tea components and brown sugar in the composition is 1:0.3:5:1.5:0.15.

[0015] Furthermore, the composition also includes an auxiliary agent, which includes one or more of a dispersant, a wetting agent, an antifreeze agent, a stabilizer, a preservative, a thickener, a defoaming agent, a solvent, an adhesive or a solid inert carrier; the total weight of the ningnanmycin, lentinan, glucan, green tea components and brown sugar is 15%-20% of the total weight of the composition.

[0016] The application of the composition is to prevent and control virus, bacteria and fungal diseases of crops, including tobacco, rice or potato.

[0017] Beneficial effects:

[0018] The pesticide composition obtained by compounding ningnanmycin, lentinan and glucan in a certain proportion has good prevention and control effects on various common viral diseases, bacterial and fungal diseases of crops such as rice and tobacco, and is safe, green and has no environmental pollution risks.

[0019] The Ningnanmycin, Lentinan and Glucan composition disclosed by the invention has good synergistic effect in preventing and controlling crop viral diseases; when the weight ratio of Ningnanmycin, Lentinan and Glucan is 1:0.3:5, the prevention effect is better.

[0020] The green tea component disclosed in the present invention also has a significant synergistic effect on the pesticide composition of ningnanmycin, lentinan and glucan components. Adding the green tea component in an appropriate proportion can significantly improve the prevention effect of the pesticide composition.

[0021] In the pesticide composition of the present invention, adding a proper amount of brown sugar component does not affect the protective effect of the pesticide composition, but has a positive effect on protecting flowers and fruits of crops, preventing frost damage, and photosynthesis and growth of leaves. DETAILED DESCRIPTION

[0022] In order to better understand the technical solution of the present invention, the embodiments of the present invention are described in detail below in conjunction with the embodiments.

[0023] It should be clear that the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] Example 1

[0025] A pesticide composition containing ningnanmycin and lentinan, the composition comprising ningnanmycin, lentinan and glucan, the weight ratio of ningnanmycin, lentinan and glucan in the composition is 1:(0.1-20):(0.1-30), and the composition is used for preventing and controlling crop viral diseases.

[0026] Furthermore, the weight ratio of Ningnanmycin, Lentinan and glucan in the composition is 1:(0.2-0.5):(4-6), and the crops include tobacco, rice or potato.

[0027] Furthermore, the weight ratio of Ningnanmycin, Lentinan and glucan in the composition is 1:0.3:5.

[0028] Furthermore, the composition also includes a green tea component, and the method for obtaining the green tea component includes: extracting green broken tea with a 60% ethanol solution to obtain an extract, the extraction temperature is 80-85°C, the weight ratio of the ethanol solution to the green broken tea is (15-18):1, evaporating the extract until there is no ethanol taste to obtain an ethanol-free water extract, adding an equal volume of ethyl acetate for extraction, extracting twice continuously, concentrating and drying the aqueous phase after the two ethyl acetate extractions to obtain the green tea component.

[0029] Furthermore, the weight ratio of Ningnanmycin, Lentinan, glucan and green tea components in the composition is 1:0.3:5:(0.5-3).

[0030] Furthermore, the weight ratio of Ningnanmycin, Lentinan, glucan and green tea components in the composition is 1:0.3:5:1.5.

[0031] Furthermore, the composition also includes brown sugar, and the weight ratio of ningnanmycin, lentinan, glucan, green tea components and brown sugar in the composition is 1:0.3:5:1.5:(0.1-0.2). The brown sugar in the present invention is a finished sugarcane sugar made from sugarcane as raw material, through juice extraction, clarification, boiling and lime method.

[0032] Furthermore, the weight ratio of Ningnanmycin, Lentinan, glucan, green tea components and brown sugar in the composition is 1:0.3:5:1.5:0.15.

[0033] Furthermore, the composition also includes an auxiliary agent, which includes one or more of a dispersant, a wetting agent, an antifreeze agent, a stabilizer, a preservative, a thickener, a defoaming agent, a solvent, an adhesive or a solid inert carrier; the total weight of the ningnanmycin, lentinan, glucan, green tea components and brown sugar is 15%-20% of the total weight of the composition.

[0034] Application of the above composition, the composition is used for preventing and controlling virus, bacteria and fungal diseases of crops, the crops include tobacco, rice or potato

[0035] Example 2

[0036] The brown sugar in this embodiment is made from sugarcane as raw material, and is a finished sugarcane sugar produced by the lime process through juice extraction, clarification, and boiling.

[0037] The adjuvants in the pesticide composition of the embodiment include emulsifiers, dispersants, wetting agents, antifreeze agents, stabilizers, preservatives, thickeners, defoamers, solvents, adhesives or solid inert carriers; all adjuvants are conventional adjuvants in the art, and the amount of adjuvants used is the conventional amount. Emulsifiers include calcium dodecylbenzene sulfonate, fatty alcohol polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, styrylphenol polyoxyethylene ether, polyoxyethylene distyrene methylphenyl ether, castor oil polyoxyethylene ether or alkylphenol ether phosphate; dispersants include sodium lignin sulfonate, naphthaldehyde condensation product sulfonate, polycarboxylate or polyoxyethylene polyoxypropylene ether block copolymer; wetting agents include sodium dodecyl sulfate, sodium lauryl sulfate, sodium dodecylbenzene sulfonate, lignin sulfonate, sodium fatty acid ester sulfate or fatty alcohol polyoxyethylene ether; antifreeze agents include ethylene glycol, polyethylene glycol, glycerol or urea. ; Stabilizers include 2,6-di-tert-butyl-4-methylphenol, triethanolamine, butylated hydroxyanisole, citric acid or sodium sorbate; preservatives include benzoic acid and its salts or sorbic acid and its salts; thickeners include sodium alginate, magnesium aluminum silicate, xanthan gum, polyethylene glycol, polyvinyl alcohol or carboxymethyl cellulose; defoaming agents include silicone oil, octanol, mineral oil or vegetable oil; binders include soluble starch, corn starch, gum arabic, polyvinyl alcohol, sucrose, lactose or glucose; solid inert carriers include diatomaceous earth, kaolin, attapulgite, bentonite, talc, white carbon black or light calcium carbonate.

[0038] The method for obtaining the green tea component in the pesticide composition is as follows: a certain mass of broken green tea is weighed, placed in a spherical flask, 15-18 times the mass of a 60% ethanol solution is added, and placed in a water bath at 80-85°C. A condenser is connected to the mouth of the spherical flask. The extraction time is 50-60 minutes, and the extraction is performed twice in succession. The extracts are combined, and the extracts are evaporated until there is no ethanol taste to obtain a deethanolized water extract, and an equal volume of ethyl acetate is added for extraction, and the extraction is performed twice in succession. The aqueous phase after extraction is concentrated and dried to obtain the green tea component.

[0039] Pesticide composition 1, the composition includes ningnanmycin, lentinan and glucan in a weight ratio of 1:0.1:30, the total weight of ningnanmycin, lentinan and glucan in the composition is 18% of the total weight of the composition, and the rest is auxiliary agent components.

[0040] Pesticide composition 2, the composition includes ningnanmycin, lentinan and glucan in a weight ratio of 1:20:0.1, the total weight of ningnanmycin, lentinan and glucan in the composition is 18% of the total weight of the composition, and the rest is auxiliary components.

[0041] Pesticide composition 3, the composition includes ningnanmycin, lentinan and glucan in a weight ratio of 1:0.3:5, the total weight of ningnanmycin, lentinan and glucan in the composition is 18% of the total weight of the composition, and the rest is auxiliary agent components.

[0042] Pesticide composition 4, the composition includes ningnanmycin, lentinan, glucan and green tea components in a weight ratio of 1:0.3:5:1.5, the total weight of ningnanmycin, lentinan, glucan and green tea components in the composition is 18% of the total weight of the composition, and the rest is auxiliary agent components.

[0043] Pesticide composition 5, the composition includes Ningnanmycin, Lentinan and green tea components in a weight ratio of 1:0.3:1.5, the total weight of Ningnanmycin, Lentinan and green tea components in the composition is 18% of the total weight of the composition, and the rest is auxiliary components.

[0044] Pesticide composition 6, the composition includes Ningnanmycin, glucan and green tea components in a weight ratio of 1:5:1.5, the total weight of Ningnanmycin, glucan and green tea components in the composition is 18% of the total weight of the composition, and the rest is auxiliary components.

[0045] Pesticide composition 7, the composition includes Ningnanmycin and Lentinan in a weight ratio of 1:0.3, the total weight of Ningnanmycin and Lentinan in the composition is 18% of the total weight of the composition, and the rest is auxiliary components.

[0046] Pesticide composition 8, the composition includes Ningnanmycin and glucan in a weight ratio of 1:5, the total weight of the Ningnanmycin and glucan components in the composition is 18% of the total weight of the composition, and the rest is auxiliary components.

[0047] Pesticide composition 9, the composition includes ningnanmycin, lentinan, glucan, green tea components and brown sugar in a weight ratio of 1:0.3:5:1.5:0.15, the total weight of ningnanmycin, lentinan, glucan, green tea components and brown sugar in the composition is 18% of the total weight of the composition, and the rest is auxiliary agent components.

[0048] The weight ratios of the components of the pesticide composition are shown in Table 1.

[0049] Table 1 Weight ratio of each component of the pesticide composition

[0050]

[0051] Field efficacy test of pesticide combination:

[0052] (1) Field efficacy trials for preventing and controlling tobacco virus diseases.

[0053] The dosage of the above pesticide composition: the pesticide is applied according to the actual content of the effective components in each pesticide composition containing Ningnanmycin and Lentinan. The investigated effective components include Ningnanmycin, Lentinan, glucan, green tea component and brown sugar, that is, the actual composition content of the effective components in each composition is calculated, and the actual mass sum of Ningnanmycin, Lentinan, glucan, green tea component and brown sugar in each composition is calculated, and the test field is applied according to the addition amount of 10 grams / mu. The experiment of the present invention is set up with 1 blank control group and 9 treatment groups. The treatment group selects the above 9 pesticide compositions, and each composition is repeated three times with a repeated area of ​​100 square meters. The tobacco is sprayed evenly with 60 kilograms of water per mu during the growth period. The tobacco virus disease disease index of each treatment group is investigated 10 days after the drug is applied. The test average value is taken for each group of treatment, and the relative control effect is calculated with the disease index of the control area. The investigation results are shown in Table 2.

[0054] Table 2 Comparison table of pesticide composition field test results

[0055] Composition Effective ingredient dosage (g / mu) 10 days after the drug was taken to prevent the disease (%) Composition 1 10 84.3 Composition 2 10 86.8 Composition 3 10 89.1 Composition 4 10 92.3 Composition 5 10 80.9 Composition 6 10 81.4 Composition 7 10 78.5 Composition 8 10 79.0 Composition 9 10 92.1 Blank control group / /

[0056] Table 2 above statistically analyzes the results of the field efficacy tests of the 9 screened compositions. By comparing the data in Table 2, it can be seen that the prevention effects of compositions 1, 2, and 3 are better than compositions 7 and 8, indicating that the prevention effect of the combination of the three effective ingredients of Ningnanmycin, Lentinan, and Glucan is better than the prevention effect of the combination of Ningnanmycin and Lentinan, and is also better than the prevention effect of the combination of Ningnanmycin and Glucan, indicating that Ningnanmycin, Lentinan, and Glucan have a synergistic effect on the efficacy; among compositions 1, 2, and 3, the prevention effect of composition 3 is significantly better than that of compositions 1 and 2, indicating that the weight ratio of Ningnanmycin, Lentinan, and Glucan in composition 3 is 1:0.3:5, which is the optimal ratio for the prevention effect of the composition; The protective effect of composition 4 is better than that of composition 3, indicating that the green tea component has a good synergistic promoting effect on the protective effect of the pesticide composition; the protective effect of composition 5 is slightly better than that of composition 7, and the difference in protective effect is not obvious; the protective effect of composition 6 is slightly better than that of composition 8, and the difference in protective effect is not obvious, indicating that the synergistic effect of the green tea component on the protective effect of the combination of ningnanmycin and lentinan and the combination of ningnanmycin and glucan is not obvious; compared with composition 9 and composition 4, the difference in protective effect between the two is not large, indicating that the addition of an appropriate amount of brown sugar component does not affect the protective effect of the pesticide composition, but in the process of studying the composition of the present invention, it is found that the addition of an appropriate amount of brown sugar component, utilizing the characteristics of the brown sugar component containing multiple amino acids and trace elements, has a positive effect on protecting flowers and fruits of crops, preventing frost damage, and photosynthesis and growth of leaves.

[0057] (2) Field efficacy test on the control of rice stripe disease.

[0058] The dosage of the above pesticide composition: the pesticide is applied according to the actual content of the effective components in each pesticide composition containing Ningnanmycin and Lentinan. The investigated effective components include Ningnanmycin, Lentinan, glucan, green tea component and brown sugar, that is, the actual composition content of the effective components in each of the above compositions is calculated, and the actual mass sum of Ningnanmycin, Lentinan, glucan, green tea component and brown sugar in the composition is calculated, and the test field is applied according to the addition amount of 8 grams / mu. The experiment of the present invention sets up a blank control group and 9 treatment groups. The pesticide composition in the treatment group selects the above 9 compositions, and each composition is repeated three times with a repeated area of ​​80 square meters. At the early stage of rice disease, 60 kilograms of water per mu are added for uniform spraying. The disease index of rice stripe disease in each treatment group is investigated 10 days after the drug is applied. The test average value is taken for each group of treatment, and the relative control effect is calculated based on the disease index of the control area. The investigation results are shown in Table 3.

[0059] Table 3 Comparison table of pesticide composition field test results

[0060]

[0061]

[0062] Table 3 above statistically analyzes the results of the field efficacy tests of the 9 compositions after screening. By comparing the statistical data results in Table 3, the control effects of compositions 1, 2, and 3 are higher than those of compositions 7 and 8, indicating that the control effect of the composition of the three effective ingredients of Ningnanmycin, Lentinan, and Glucan is better than the control effect of the composition of the two components of Ningnanmycin and Lentinan, and also better than the control effect of the composition of the two components of Ningnanmycin and Glucan. It also shows that there is a synergistic promotion effect between the three components of Ningnanmycin, Lentinan, and Glucan in terms of efficacy; among compositions 1, 2, and 3, the control effect of composition 3 is significantly better than that of compositions 1 and 2, indicating that the weight ratio of Ningnanmycin, Lentinan, and Glucan in composition 3 is 1:0.3: 5 is the optimized ratio of the control effect of the combination; the control effect of composition 4 is significantly better than that of composition 3, indicating that the green tea component has a good synergistic promoting effect on the control effect of the three-component combination of ningnanmycin, lentinan and glucan; the control effect of composition 5 is slightly better than that of composition 7, and the control effect of composition 6 is slightly better than that of composition 8, indicating that the green tea component has a certain synergistic effect on the combination of ningnanmycin and lentinan, as well as the combination of ningnanmycin and glucan in preventing and controlling rice stripe leaf blight; compared with composition 4, the control effect of composition 9 is not much different, indicating that the addition of an appropriate amount of brown sugar component does not affect the control effect of the pesticide composition, but the addition of an appropriate amount of brown sugar component has a positive effect on protecting flowers and fruits of crops, preventing frost damage, and photosynthesis and growth of leaves.

[0063] The embodiments of the present invention are described in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A pesticide composition containing Ningnanmycin and Lentinan, characterized in that: The composition comprises ningnanmycin, lentinan and glucan, and the weight ratio of ningnanmycin, lentinan and glucan in the composition is 1:(0.1-20):(0.1-30).

2. The composition according to claim 1, characterized in that The weight ratio of Ningnanmycin, Lentinan and Dextran in the composition is 1:(0.2-0.5):(4-6).

3. The composition according to claim 2, characterized in that The weight ratio of Ningnanmycin, Lentinan and glucan in the composition is 1:0.3:

5.

4. The composition according to claim 3, characterized in that The composition also includes a green tea component, and a method for obtaining the green tea component includes: extracting green broken tea with a 60% ethanol solution to obtain an extract, the extraction temperature is 80-85°C, the weight ratio of the ethanol solution to the green broken tea is (15-18):1, evaporating the extract until there is no ethanol taste to obtain an ethanol-free water extract, adding an equal volume of ethyl acetate for extraction, extracting twice continuously, concentrating and drying the aqueous phase after the two ethyl acetate extractions to obtain the green tea component.

5. The composition according to claim 4, characterized in that The weight ratio of Ningnanmycin, Lentinan, glucan and green tea components in the composition is 1:0.3:5:(0.5-3).

6. The composition according to claim 5, characterized in that The weight ratio of Ningnanmycin, Lentinan, glucan and green tea components in the composition is 1:0.3:5:1.

5.

7. The composition according to claim 6, characterized in that The composition also includes brown sugar. The weight ratio of ningnanmycin, lentinan, polysaccharide, green tea components and brown sugar in the composition is 1:0.3:5:1.5:(0.1-0.2).

8. The composition according to claim 7, characterized in that The weight ratio of Ningnanmycin, Lentinan, glucan, green tea components and brown sugar in the composition is 1:0.3:5:1.5:0.

15.

9. The composition according to claim 8, characterized in that The composition also includes an auxiliary agent, which includes one or more of a dispersant, a wetting agent, an antifreeze agent, a stabilizer, a preservative, a thickener, a defoaming agent, a solvent, an adhesive or a solid inert carrier; the total weight of the ningnanmycin, lentinan, glucan, green tea components and brown sugar is 15%-20% of the total weight of the composition.

10. Use of the composition according to any one of claims 1 to 9, characterized in that: The composition is used for preventing and controlling virus, bacteria and fungal diseases of crops.

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