Traditional Chinese medicine composition, repair cream for repairing various scars and preparation method of repair cream

Abstract

The invention discloses a traditional Chinese medicine composition, repair cream for repairing various scars and a preparation method of the repair cream. The repairing paste comprises a traditional Chinese medicine composition, and the traditional Chinese medicine composition comprises the following components in parts by weight: 20-30 parts of airpotato yam rhizome, 20-30 parts of herba violae, 20-30 parts of radix stemonae japonicae, 18-25 parts of plantain herb, 15-20 parts of corydalis saxicola bunting, 15-20 parts of common andrographis herb, 15-20 parts of verbena, 10-15 parts of paris polyphylla, 10-15 parts of ligusticum chuanxiong hort, 10-15 parts of radix achyranthis bidentatae, 15-20 parts of centella asiatica, 8-12 parts of honeysuckle stem and 8-12 parts of salvia miltiorrhiza. Wherein the airpotato yam rhizome and the corydalis saxicola bunting are subjected to compound probiotic fermentation treatment. According to the traditional Chinese medicine composition, airpotato yam rhizome and corydalis saxicola bunting are subjected to specific bifidobacterium longum CICC 25033 and lactobacillus plantarum CICC 6053 synergistic fermentation and then have a synergistic effect with centella asiatica, the effect of remarkably inhibiting rabbit ear HS is achieved, and therefore the effect of repairing scars is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of scar lightening repairing cream, in particular to a Chinese medicine composition, a repairing cream for repairing various scars and a preparation method thereof. Background Art

[0002] Hypertrophic scar (HS) is the result of poor wound healing after dermal tissue damage. It is also a common pathological scar in clinical practice, usually secondary to surgery, trauma, burns and infection. Clinically, there are a variety of treatments for HS, mainly surgical resection, compression therapy, freezing, laser irradiation, etc. At present, surgical resection is still one of the main treatments, and it can achieve certain results for most HS. However, the therapeutic effect of surgical resection is very limited, and the surgery itself is a secondary injury, and the probability of hypertrophic scar reappearance is very high. Therefore, it is urgent to find an effective means to treat HS and break through the current bottleneck. Based on the many advantages of traditional Chinese medicine, this study developed a repair cream through the combination of traditional Chinese medicine and Chinese herbal fermentation technology, which has significant effects on reducing hypertrophic scars and repairing scars. Summary of the invention

[0003] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a Chinese medicine composition, a repairing cream for repairing various scars and a preparation method thereof, so as to solve the problems raised in the above technical background.

[0004] To achieve the above object, the present invention is implemented through the following technical solutions:

[0005] In a first aspect, the present invention provides a traditional Chinese medicine composition comprising the following ingredients by weight: 20-30 parts of Dioscorea bulbifera, 20-30 parts of Viola yedoensis, 20-30 parts of Stemona ramosissima, 18-25 parts of Plantago, 15-20 parts of Coptis chinensis, 15-20 parts of Andrographis paniculata, 15-20 parts of Verbena officinalis, 10-15 parts of Paridis australis, 10-15 parts of Chuanxiong, 10-15 parts of Achyranthes bidentata, 15-20 parts of Centella asiatica, 8-12 parts of Lonicera japonica, and 8-12 parts of Salvia miltiorrhiza; wherein Dioscorea bulbifera and Coptis chinensis are fermented with composite probiotics.

[0006] Furthermore, a Chinese medicine composition comprises the following ingredients by weight: 25 parts of Dioscorea bulbifera, 25 parts of Viola yedoensis, 25 parts of Stemona roxburghii, 20 parts of Plantago, 18 parts of Coptis chinensis, 18 parts of Andrographis paniculata, 18 parts of Verbena officinalis, 12 parts of Polygonum multiflorum, 12 parts of Chuanxiong, 12 parts of Achyranthes bidentata, 18 parts of Centella asiatica, 10 parts of Lonicera japonica, and 10 parts of Salvia miltiorrhiza.

[0007] Furthermore, the composite probiotics comprises Bifidobacterium longum CICC 25033 and Lactobacillus plantarum CICC 6053.

[0008] In a second aspect, the present invention provides a repairing cream for repairing various types of scars, comprising the above-mentioned Chinese medicine composition.

[0009] In a third aspect, the present invention provides a method for preparing a repairing cream for repairing various scars, comprising the following steps:

[0010] S1. Weigh 20-30 parts of Dioscorea bulbifera, 20-30 parts of Viola yedoensis, 20-30 parts of Stemona ramosissima, 18-25 parts of Plantago, 15-20 parts of Coptis chinensis, 15-20 parts of Andrographis paniculata, 15-20 parts of Verbena officinalis, 10-15 parts of Paridis aestivum, 10-15 parts of Chuanxiong, 10-15 parts of Achyranthes bidentata, 15-20 parts of Centella asiatica, 8-12 parts of Honeysuckle, 8-12 parts of Salvia miltiorrhiza, 60-90 parts of Vaseline, and 4 parts of Borneol by weight;

[0011] S2, wash and dry Dioscorea bulbifera, Viola yedoensis, Stemona ramosissima, Plantago, Coptis chinensis, Andrographis paniculata, Verbena officinalis, Polygonum multiflorum, Chuanxiong, Achyranthes bidentata, Centella asiatica, Honeysuckle vine, and Salvia miltiorrhiza, and grind them into 80-100 mesh particles respectively;

[0012] S3. Prepare bacterial liquid of Bifidobacterium longum CICC 25033 and Lactobacillus plantarum CICC 6053. The specific method is as follows: activate Bifidobacterium longum CICC 25033 according to the conventional method, inoculate the activated Bifidobacterium longum CICC 25033 into a shake flask containing 1000 mL of MRS liquid culture medium, and ferment for 10-18 hours to obtain a concentration of 1.5×10 8 ~4.5×10 8 cfu / mL of Bifidobacterium longum CICC 25033 bacterial liquid; Lactobacillus plantarum CICC 6053 was activated as usual, and the activated Lactobacillus plantarum CICC 6053 was inoculated into a shake flask containing 1000 mL of LMRS liquid culture medium and fermented for 10-18 hours to obtain a concentration of

[0013] 2.0×10 8 ~5.5×10 8 cfu / mL Lactobacillus plantarum CICC 6053 bacterial solution;

[0014] Among them, MRS medium: casein peptone 10.0g, beef extract 10.0g, yeast powder 5.0g, glucose 5.0g, sodium acetate 5.0g, diammonium citrate 2.0g, Tween 80 1.0g, K2HPO4 2.0g, MgSO4.7H2O 0.2g, MnSO4.H2O 0.05g, CaCO3 20.0g, agar 15.0g, distilled water 1.0L, pH6.8.

[0015] S4, after mixing Dioscorea bulbifera and Coptis chinensis evenly, add Bifidobacterium longum CICC 25033 bacterial solution at an inoculum amount of 2-5%, mix and ferment in a cylinder, perform anaerobic fermentation at a temperature of 36-37° C. for 10 to 24 hours; then add Lactobacillus plantarum CICC 6053 bacterial solution at an inoculum amount of 2-5% of the total mass of Dioscorea bulbifera and Coptis chinensis, perform aerobic fermentation at a temperature of 37° C. for 10 to 24 hours, and after the fermentation is completed, add 3% hydrogen peroxide to inactivate Bifidobacterium longum and Lactobacillus plantarum CICC6053 to obtain a fermented product;

[0016] S5, take the fermented product obtained in S3 and add 70% ethanol 10 times the amount, reflux extraction for 30 minutes, repeat 3 times, discard the residue, combine the filtrate, recover the alcohol, and concentrate until there is no alcohol taste, and obtain the fermented product extract for use;

[0017] S6. Take Centella asiatica, add 10 times the amount of 70% ethanol, reflux extraction for 30 minutes, repeat 3 times, discard the residue, combine the filtrate, recover the alcohol, and concentrate until there is no alcohol taste, and obtain the Centella asiatica extract for use;

[0018] S7, the Chinese medicinal materials except Dioscorea bulbifera, Coptis chinensis and Centella asiatica in step S2 are mixed evenly, 10 times the amount of water is added, and the mixture is decocted for 40 min, and the filtrate is filtered and taken, and the mixture is repeated 3 times, and the filtrates are combined and concentrated to a relative density of 1.1-1.2 (60° C.) to obtain a Chinese medicinal concentrate;

[0019] S8. Heat the vaseline to melt and keep it warm, then add the fermentation extract obtained in step S5, the Centella asiatica extract obtained in step S6 and the Chinese medicine concentrate obtained in step S7 while stirring, continue stirring until it becomes viscous, let it cool naturally, then add the ground borneol while stirring, and mix thoroughly to obtain a plaster.

[0020] Further, the concentration of 3.0×10 8 cfu / mL of Bifidobacterium longum CICC 25033 and a concentration of 3.6×10 8 cfu / mL Lactobacillus plantarum CICC 6053 bacterial solution.

[0021] Furthermore, the inoculation amount of the bacterial solution of Bifidobacterium longum CICC 25033 and the bacterial solution of Lactobacillus plantarum CICC 6053 is 3% of the total mass of Dioscorea bulbifera and Coptis chinensis.

[0022] Furthermore, in step S6, vaseline is heated to 80°C to melt and keep warm, and then the medicinal oil is added while stirring, and then medical zinc oxide powder is added while stirring, and it is allowed to cool naturally. After cooling to a temperature of 60°C, borneol is added while stirring, and the plaster is obtained after being fully mixed.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. In the present invention, after the synergistic fermentation of Dioscorea bulbifera and Coptis chinensis with specific Bifidobacterium longum CICC 25033 and Lactobacillus plantarum CICC 6053, the synergistic effect is achieved with Centella asiatica and other components of the obtained repair cream. The obtained repair cream has a significantly enhanced effect of inhibiting the formation of HS in rabbit ears, which is much higher than the repair cream obtained by fermenting Dioscorea bulbifera and Coptis chinensis with a single bacterial agent, or Bifidobacterium longum CICC 25033 and other Lactobacillus plantarum with Centella asiatica and other components.

[0025] 2. The repairing cream provided by the present invention is used for the prevention and treatment of traumatic scars, with an effective rate of 99% (1022 / 1032 cases) and a scar flattening cure rate of 62% (639 / 1032 cases).

[0026] 3. Use Chinese medicine (repair cream) for non-invasive combined prevention and treatment of scars, which has the characteristics of early intervention time, short treatment cycle, no trauma, no injection, no side effects, no medical risks, and clinical endpoints infinitely close to normal skin.

[0027] 4. The repairing cream of the present invention has the functions of promoting blood circulation and removing blood stasis, clearing away heat and detoxifying, softening nodules and dispersing hard masses, flattening scars and repairing them, accelerating the metabolism of scar connective tissue, and sealing capillaries for blood supply and nourishment. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a bar graph showing the effects of different repair creams on the thickness of HS scars in rabbit ears. DETAILED DESCRIPTION

[0029] The following describes the embodiments of the present invention by specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict.

[0030] In the present invention, Bifidobacterium longum CICC 25033, Lactobacillus plantarum CICC 6053, and Lactobacillus plantarum CICC24939 were all purchased from Shanghai Chen Microbiology Technology Co., Ltd.

[0031] Example 1

[0032] A traditional Chinese medicine composition comprises the following ingredients in parts by weight: 20 parts of Dioscorea bulbifera, 20 parts of Viola yedoensis, 30 parts of Stemona ramosissima, 25 parts of Plantago, 20 parts of Coptis chinensis, 20 parts of Andrographis paniculata, 15 parts of Verbena officinalis, 10 parts of Paridis aestivum, 10 parts of Chuanxiong, 15 parts of Achyranthes bidentata, 20 parts of Centella asiatica, 8 parts of Honeysuckle Caulis, and 12 parts of Salvia miltiorrhiza; wherein the Dioscorea bulbifera and Coptis chinensis are fermented with composite probiotics, and the composite probiotics comprise Bifidobacterium longum CICC 25033 and Lactobacillus plantarum CICC 6053.

[0033] Example 2

[0034] A traditional Chinese medicine composition comprises the following ingredients in parts by weight: 30 parts of Dioscorea bulbifera, 30 parts of Viola yedoensis, 20 parts of Stemona ramosissima, 18 parts of Plantago, 15 parts of Coptis chinensis, 15 parts of Andrographis paniculata, 20 parts of Verbena officinalis, 15 parts of Paridis aestivum, 15 parts of Chuanxiong, 10 parts of Achyranthes bidentata, 15 parts of Centella asiatica, 12 parts of Honeysuckle Caulis, and 8 parts of Salvia miltiorrhiza; wherein the Dioscorea bulbifera and Coptis chinensis are fermented with composite probiotics, and the composite probiotics comprise Bifidobacterium longum CICC 25033 and Lactobacillus plantarum CICC 6053.

[0035] Example 3

[0036] A Chinese medicine composition comprises the following ingredients by weight: 25 parts of Dioscorea bulbifera, 25 parts of Viola yedoensis, 25 parts of Stemona ramosissima, 20 parts of Plantago, 18 parts of Coptis chinensis, 18 parts of Andrographis paniculata, 18 parts of Verbena officinalis, 12 parts of Paridis septififlora, 12 parts of Chuanxiong, 12 parts of Achyranthes bidentata, 18 parts of Centella asiatica, 10 parts of Honeysuckle, and 10 parts of Salvia miltiorrhiza. Dioscorea bulbifera and Coptis chinensis are fermented with composite probiotics, and the composite probiotics include Bifidobacterium longum CICC 25033 and Lactobacillus plantarum CICC 6053.

[0037] Example 4

[0038] A repairing cream for repairing various scars, comprising the Chinese medicine composition described in Example 1; and a preparation method thereof comprises the following steps:

[0039] S1. Weigh 20 parts of Dioscorea bulbifera, 20 parts of Viola yedoensis, 30 parts of Stemona ramosissima, 25 parts of Plantago, 20 parts of Coptis chinensis, 20 parts of Andrographis paniculata, 15 parts of Verbena officinalis, 10 parts of Paridis aestivum, 10 parts of Chuanxiong, 15 parts of Achyranthes bidentata, 20 parts of Centella asiatica, 8 parts of Honeysuckle, 12 parts of Salvia miltiorrhiza, 75 parts of Vaseline, and 4 parts of Borneol by weight;

[0040] S2, wash and dry Dioscorea bulbifera, Viola yedoensis, Stemona ramosissima, Plantago, Coptis chinensis, Andrographis paniculata, Verbena officinalis, Polygonum multiflorum, Chuanxiong, Achyranthes bidentata, Centella asiatica, Honeysuckle vine, and Salvia miltiorrhiza, and grind them into 100 mesh particles respectively;

[0041] S3, prepare bacterial liquid of Bifidobacterium longum CICC 25033 and Lactobacillus plantarum CICC 6053. The specific method is: activate Bifidobacterium longum CICC 25033 according to the routine method, inoculate the activated Bifidobacterium longum CICC 25033 into a shake flask containing 1000 mL of LMRs liquid culture medium, and ferment for 10 hours to obtain a concentration of 1.5×10 8 cfu / mL of Bifidobacterium longum CICC25033 bacterial liquid; Lactobacillus plantarum CICC 6053 was activated as usual, and the activated Lactobacillus plantarum CICC 6053 was inoculated into a shake flask containing 1000mL MRS liquid culture medium and fermented for 18h to obtain a concentration of 5.5×10 8 cfu / mL Lactobacillus plantarum CICC 6053 bacterial solution;

[0042] S4, after mixing the Rhizoma Dioscoreae Bulbiferae and Rhizoma Coptis chinensis uniformly, add the bacterial solution of Bifidobacterium longum CICC 25033 at an inoculum amount of 5%, mix and ferment in a cylinder, perform anaerobic fermentation at a temperature of 36°C for 10 hours; then add the bacterial solution of Lactobacillus plantarum CICC 6053 at an inoculum amount of 2% of the total mass of Rhizoma Dioscoreae Bulbiferae and Rhizoma Coptis chinensis, perform aerobic fermentation at a temperature of 37°C for 24 hours, and after the fermentation is completed, add 3% hydrogen peroxide to inactivate Bifidobacterium longum and Lactobacillus plantarum CICC 6053 to obtain a fermented product;

[0043] S5, take the fermented product obtained in S3 and add 70% ethanol 10 times the amount, reflux extraction for 30 minutes, repeat 3 times, discard the residue, combine the filtrate, recover the alcohol, and concentrate until there is no alcohol taste, and obtain the fermented product extract for use;

[0044] S6. Take Centella asiatica, add 10 times the amount of 70% ethanol, reflux extraction for 30 minutes, repeat 3 times, discard the residue, combine the filtrate, recover the alcohol, and concentrate until there is no alcohol taste, and obtain the Centella asiatica extract for use;

[0045] S7, the Chinese medicinal materials except Dioscorea bulbifera, Coptis chinensis and Centella asiatica in step S2 are mixed evenly, 10 times the amount of water is added, and the mixture is decocted for 40 min, and the filtrate is filtered and taken, and the mixture is repeated 3 times, and the filtrates are combined and concentrated to a relative density of 1.2 (60° C.) to obtain a Chinese medicinal concentrate;

[0046] S8. Heat the vaseline to melt and keep it warm, then add the fermentation extract obtained in step S5, the Centella asiatica extract obtained in step S6 and the Chinese medicine concentrate obtained in step S7 while stirring, continue stirring until it becomes viscous, let it cool naturally, then add the ground borneol while stirring, and mix thoroughly to obtain a plaster.

[0047] Example 5

[0048] A repairing cream for repairing various scars, comprising the Chinese medicine composition described in Example 2; and a preparation method thereof comprises the following steps:

[0049] S1. Weigh 30 parts of Dioscorea bulbifera, 30 parts of Viola yedoensis, 20 parts of Stemona ramosissima, 18 parts of Plantago, 15 parts of Coptis chinensis, 15 parts of Andrographis paniculata, 20 parts of Verbena officinalis, 15 parts of Paridis aestivum, 15 parts of Chuanxiong, 10 parts of Achyranthes bidentata, 15 parts of Centella asiatica, 12 parts of Honeysuckle, 8 parts of Salvia miltiorrhiza, 75 parts of Vaseline, and 4 parts of Borneol by weight;

[0050] S2, wash and dry Dioscorea bulbifera, Viola yedoensis, Stemona ramosissima, Plantago, Coptis chinensis, Andrographis paniculata, Verbena officinalis, Polygonum multiflorum, Chuanxiong, Achyranthes bidentata, Centella asiatica, Honeysuckle vine, and Salvia miltiorrhiza, and grind them into 100 mesh particles respectively;

[0051] S3, prepare bacterial liquid of Bifidobacterium longum CICC 25033 and Lactobacillus plantarum CICC 6053. The specific method is as follows: activate Bifidobacterium longum CICC 25033 according to the routine method, inoculate the activated Bifidobacterium longum CICC 25033 into a shake flask containing 1000 mL of MRS liquid culture medium, and ferment for 18 hours to obtain a concentration of 4.5×10 8 cfu / mL of Bifidobacterium longum CICC 25033 culture liquid; Lactobacillus plantarum CICC 6053 was activated according to the routine method, and the activated Lactobacillus plantarum CICC 6053 was inoculated into a shake flask containing 1000mL MRS liquid culture medium and fermented for 10h to obtain a concentration of 2.0×10 8 cfu / mL Lactobacillus plantarum CICC 6053 bacterial solution;

[0052] S4, after mixing the Rhizoma Dioscoreae Bulbiferae and Rhizoma Coptis chinensis uniformly, add the bacterial solution of Bifidobacterium longum CICC 25033 at an inoculum amount of 2% and mix them, and then ferment them in a cylinder for anaerobic fermentation at a temperature of 36° C. for 24 hours; then add the bacterial solution of Lactobacillus plantarum CICC 6053 at an inoculum amount of 5% of the total mass of Rhizoma Dioscoreae Bulbiferae and Rhizoma Coptis chinensis, and perform aerobic fermentation at a temperature of 37° C. for 10 hours. After the fermentation is completed, add 3% hydrogen peroxide to inactivate Bifidobacterium longum and Lactobacillus plantarum CICC 6053 to obtain a fermented product;

[0053] S5, take the fermented product obtained in S3 and add 70% ethanol 10 times the amount, reflux extraction for 30 minutes, repeat 3 times, discard the residue, combine the filtrate, recover the alcohol, and concentrate until there is no alcohol taste, and obtain the fermented product extract for use;

[0054] S6. Take Centella asiatica, add 10 times the amount of 70% ethanol, reflux extraction for 30 minutes, repeat 3 times, discard the residue, combine the filtrate, recover the alcohol, and concentrate until there is no alcohol taste, and obtain the Centella asiatica extract for use;

[0055] S7, the Chinese medicinal materials except Dioscorea bulbifera, Coptis chinensis and Centella asiatica in step S2 are mixed evenly, 10 times the amount of water is added, and the mixture is decocted for 40 min, and the filtrate is filtered and taken, and the mixture is repeated 3 times, and the filtrates are combined and concentrated to a relative density of 1.2 (60° C.) to obtain a Chinese medicinal concentrate;

[0056] S8. Heat the vaseline to melt and keep it warm, then add the fermentation extract obtained in step S5, the Centella asiatica extract obtained in step S6 and the Chinese medicine concentrate obtained in step S7 while stirring, continue stirring until it becomes viscous, let it cool naturally, then add the ground borneol while stirring, and mix thoroughly to obtain a plaster.

[0057] Example 6

[0058] A repairing cream for repairing various scars, comprising the Chinese medicine composition described in Example 3; and a preparation method thereof comprises the following steps:

[0059] S1. Weigh 25 parts of Dioscorea bulbifera, 25 parts of Viola yedoensis, 25 parts of Stemona ramosissima, 20 parts of Plantago, 18 parts of Coptis chinensis, 18 parts of Andrographis paniculata, 18 parts of Verbena officinalis, 12 parts of Paridis aestivum, 12 parts of Chuanxiong, 12 parts of Achyranthes bidentata, 18 parts of Centella asiatica, 10 parts of Honeysuckle Caulis, 10 parts of Salvia miltiorrhiza, 75 parts of Vaseline, and 4 parts of Borneol by weight;

[0060] S2, wash and dry Dioscorea bulbifera, Viola yedoensis, Stemona ramosissima, Plantago, Coptis chinensis, Andrographis paniculata, Verbena officinalis, Polygonum multiflorum, Chuanxiong, Achyranthes bidentata, Centella asiatica, Honeysuckle vine, and Salvia miltiorrhiza, and grind them into 100 mesh particles respectively;

[0061] S3, preparing bacterial liquid of Bifidobacterium longum CICC 25033 and Lactobacillus plantarum CICC 6053. The specific method is as follows: Bifidobacterium longum CICC 25033 is activated according to the conventional method, and the activated Bifidobacterium longum CICC 25033 is inoculated into a shake flask containing 1000 mL of MRS liquid culture medium and fermented for 14 hours to obtain a concentration of 3.0×10 8 cfu / mL of Bifidobacterium longum CICC 25033 culture liquid; Lactobacillus plantarum CICC 6053 was activated according to the routine method, and the activated Lactobacillus plantarum CICC 6053 was inoculated into a shake flask containing 1000mL MRS liquid culture medium and fermented for 14h to obtain a concentration of 3.6×10 8 cfu / mL Lactobacillus plantarum CICC 6053 bacterial solution;

[0062] S4, after mixing the Rhizoma Dioscoreae Bulbiferae and Rhizoma Coptis chinensis uniformly, adding the bacterial solution of Bifidobacterium longum CICC 25033 at an inoculum amount of 3% and mixing, the mixture was put into a cylinder for fermentation, and the anaerobic fermentation was carried out at a temperature of 36° C. for 18 hours; then, adding the bacterial solution of Lactobacillus plantarum CICC 6053 at an inoculum amount of 3% of the total mass of Rhizoma Dioscoreae Bulbiferae and Rhizoma Coptis chinensis, and aerobic fermentation was carried out at a temperature of 37° C. for 18 hours, and after the fermentation was completed, 3% hydrogen peroxide was added to inactivate Bifidobacterium longum and Lactobacillus plantarum CICC 6053 to obtain a fermented product;

[0063] S5, take the fermented product obtained in S3 and add 70% ethanol 10 times the amount, reflux extraction for 30 minutes, repeat 3 times, discard the residue, combine the filtrate, recover the alcohol, and concentrate until there is no alcohol taste, and obtain the fermented product extract for use;

[0064] S6. Take Centella asiatica, add 10 times the amount of 70% ethanol, reflux extraction for 30 minutes, repeat 3 times, discard the residue, combine the filtrate, recover the alcohol, and concentrate until there is no alcohol taste, and obtain the Centella asiatica extract for use;

[0065] S7, the Chinese medicinal materials except Dioscorea bulbifera, Coptis chinensis and Centella asiatica in step S2 are mixed evenly, 10 times the amount of water is added, and the mixture is decocted for 40 min, and the filtrate is filtered and taken, and the mixture is repeated 3 times, and the filtrates are combined and concentrated to a relative density of 1.2 (60° C.) to obtain a Chinese medicinal concentrate;

[0066] S8. Heat the vaseline to melt and keep it warm, then add the fermentation extract obtained in step S5, the Centella asiatica extract obtained in step S6 and the Chinese medicine concentrate obtained in step S7 while stirring, continue stirring until it becomes viscous, let it cool naturally, then add the ground borneol while stirring, and mix thoroughly to obtain a plaster.

[0067] Comparative Example 1

[0068] This comparative example is similar to Example 6, except that only Bifidobacterium longum CICC 25033 is used for fermentation in step S4; the rest is the same as Example 6.

[0069] Comparative Example 2

[0070] This comparative example is similar to Example 6, except that only Lactobacillus plantarum CICC 6053 is used for fermentation in step S4; the rest is the same as Example 6.

[0071] Comparative Example 3

[0072] This comparative example is similar to Example 6, except that Bifidobacterium longum CICC 25033 and other Lactobacillus plantarum are used for fermentation in step S4; the rest is the same as Example 6. The other Lactobacillus plantarum is Lactobacillus plantarum CICC 24939, which is purchased from Shanghai Chen Microbiology Technology Co., Ltd.

[0073] Comparative Example 4

[0074] This comparative example is similar to Example 6, except that the Rhizoma Dioscoreae Bulbifera and Rhizoma Coptis Chinensis in step S4 are not fermented, and the alcohol extraction in step S5 is directly performed; the rest is the same as Example 6.

[0075] Comparative Example 5

[0076] This comparative example is similar to Example 6, except that no Centella asiatica extract is added in step S8; the rest is the same as Example 6.

[0077] Effect of repair cream on hypertrophic scars

[0078] Experimental grouping: 54 New Zealand white rabbits with healthy ears were randomly divided into 9 groups, 6 rabbits in each group, namely the model group, Example 4-6 groups, and Comparative Example 1-5 groups;

[0079] Test method:

[0080] Rabbit ear hypertrophic scar (HS) modeling: 54 New Zealand white rabbits with healthy ears were selected, each of which was housed in a single cage under standard conditions and fed adaptively for 1 week. Anesthesia was performed by intraperitoneal injection of 5% pentobarbital 1 ml / kg, and the rabbit ear abdomen was routinely disinfected with iodine. Three circular wounds with a diameter of 1 cm were punched along both sides of the rabbit ear abdomen with a puncher, and the distance between each wound was greater than 5 mm. The skin and subcutaneous tissue were cut along the wound with a sterile blade, and the perichondrium was carefully peeled off with forceps and ophthalmic scissors to retain the cartilage. The wound was disinfected again, and sterile gauze was used to press and stop bleeding. The gauze was removed after 1 day, and the wound secretions were cleared.

[0081] Treatment of rabbit ear HS: One week after modeling, the wounds were all epithelialized, and therapeutic intervention was performed on the rabbit ear wounds at this time. Starting from the 8th day after surgery, the model group did not receive treatment, and the Example 4-6 group and the Comparative Example 1-5 group were smeared with the corresponding repair cream, and the medication was given once a day. After medication, the skin around the wound was wiped to keep it clean and dry. After 4 weeks of treatment, the wounds of the rabbit ears of each group were observed; at the same time, the big-eared white rabbits were anesthetized and killed, and the scar tissue of the rabbit ears of each group was cut. The cutting depth was from the surface scar tissue to the cartilage layer, and the scar thickness of each group was measured with a vernier caliper. Records were kept and statistics were made. The results are shown in Tables 1 and Figure 1 shown.

[0082] Table 1

[0083]

[0084] From Table 1 and Figure 1It can be seen that after 4 weeks of treatment, it can be seen that the HS of the rabbit ears in the model group is significantly more prominent than the surrounding normal skin, with a rough surface and a tough texture, and is in the form of a hard nodule; while the HS of the rabbit ears in the Example 4-6 group is significantly thinner and closer to the surrounding skin, and most of the HS tissues are basically as flat as the surrounding skin. The scar thickness of all cut rabbit ear HS was measured, and the results showed that compared with the model group, the scar thickness of the Example 4-6 group and the Comparative Example 1-5 group was significantly reduced, and compared with the Comparative Example 1-5 group, the scar thickness of the Example 4-6 group was significantly reduced, which shows that Example 4-6 can significantly inhibit the formation of rabbit ear HS and has the effect of repairing scars. This is because after the specific long Bifidobacterium CICC 25033 bacterial liquid and Lactobacillus plantarum CICC 6053 synergistic fermentation of Dioscorea bulbifera and Coptis chinensis, they work synergistically with Centella asiatica, and have the effect of significantly inhibiting rabbit ear HS.

[0085] Application Examples

[0086] The research team of this application has been engaged in the prevention and treatment of traumatic scars since 2005. Scar prevention and control is to reduce the risk of scar hyperplasia caused by therapeutic factors. After skin trauma, it quickly inhibits the excessive proliferation of scar collagen fibers, promotes the wound to heal just right in a short period of time, accelerates wound healing, and avoids the appearance of uneven wounds, redness and congestion, tough texture, itching and burning, and irregular scar external morphology, which affect the appearance. The prevention and control plan has the following characteristics: early intervention time, short treatment cycle, no trauma, no injection, no side effects, no medical risks, and clinical endpoints are infinitely close to normal skin. Since 2005, a total of more than 5,000 cases have been treated.

[0087] Traditional Chinese medicine non-invasive combined treatment for scar prevention and control program: Intervention treatment is carried out at the early stage of the wound, the sooner the better. The Chinese medicine ointment with a self-developed Chinese medicine formula (the repair ointment obtained in Example 6) acts on the open wound and is used in the early stage of the wound to intervene in the whole process of wound healing to prevent excessive proliferation of fibroblasts to form more abnormal fibroblasts, thereby forming scars. The statistical analysis of 1032 cases in the three years from 2021 to 2024 is as follows:

[0088] Among them, 1022 cases were effective, accounting for 99%; 639 cases were cured, accounting for 62%; effective means that the scar has improved, and cured means that the scar is healed and flattened;

[0089] The uncured rate was due to economic reasons: 217 cases accounted for 21%; due to distance reasons: 93 cases accounted for 9%; due to primary keloids: 83 cases accounted for 8%;

[0090] Among them, drug allergy accounted for 3 cases, accounting for 0.3%; adhesive tape allergy accounted for 2%.

[0091] The above-mentioned embodiments only express the specific implementation of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A Chinese medicine composition, characterized in that: The composition comprises the following ingredients by weight: 20-30 parts of Dioscorea bulbifera, 20-30 parts of Viola yedoensis, 20-30 parts of Stemona ramosissima, 18-25 parts of Plantago, 15-20 parts of Coptis chinensis, 15-20 parts of Andrographis paniculata, 15-20 parts of Verbena officinalis, 10-15 parts of Paridis aestivum, 10-15 parts of Chuanxiong, 10-15 parts of Achyranthes bidentata, 15-20 parts of Centella asiatica, 8-12 parts of Lonicera japonica, and 8-12 parts of Salvia miltiorrhiza; wherein Dioscorea bulbifera and Coptis chinensis are fermented with composite probiotics.

2. A Chinese medicine composition according to claim 1, characterized in that: The composition comprises the following ingredients by weight: 25 parts of Dioscorea bulbifera, 25 parts of Viola yedoensis, 25 parts of Stemona ramosissima, 20 parts of Plantago, 18 parts of Coptis chinensis, 18 parts of Andrographis paniculata, 18 parts of Verbena officinalis, 12 parts of Polygonum multiflorum, 12 parts of Chuanxiong, 12 parts of Achyranthes bidentata, 18 parts of Centella asiatica, 10 parts of Honeysuckle vine, and 10 parts of Salvia miltiorrhiza.

3. A Chinese medicine composition according to claim 1, characterized in that: The composite probiotics comprises Bifidobacterium longum CICC 25033 and Lactobacillus plantarum CICC 6053.

4. A repairing cream for repairing various scars, characterized in that: A Chinese medicine composition comprising any one of claims 1 to 3.

5. The method for preparing the repairing cream for repairing various scars according to claim 4, characterized in that: The following steps are involved: S1. Weigh 20-30 parts of Dioscorea bulbifera, 20-30 parts of Viola yedoensis, 20-30 parts of Stemona ramosissima, 18-25 parts of Plantago, 15-20 parts of Coptis chinensis, 15-20 parts of Andrographis paniculata, 15-20 parts of Verbena officinalis, 10-15 parts of Paridis aestivum, 10-15 parts of Chuanxiong, 10-15 parts of Achyranthes bidentata, 15-20 parts of Centella asiatica, 8-12 parts of Honeysuckle, 8-12 parts of Salvia miltiorrhiza, 60-90 parts of Vaseline, and 4 parts of Borneol by weight; S2, wash and dry Dioscorea bulbifera, Viola yedoensis, Stemona ramosissima, Plantago, Coptis chinensis, Andrographis paniculata, Verbena officinalis, Polygonum multiflorum, Chuanxiong, Achyranthes bidentata, Centella asiatica, Honeysuckle vine, and Salvia miltiorrhiza, and grind them into 80-100 mesh particles respectively; S3, prepared at a concentration of 1.5×10 8 ~4.5×10 8 cfu / mL of Bifidobacterium longum CICC 25033 and a concentration of 2.0×10 8 ~5.5×10 8 cfu / mL Lactobacillus plantarum CICC 6053 bacterial solution; S4, after mixing the Rhizoma Dioscoreae Bulbiferae and Rhizoma Coptis chinensis uniformly, adding the bacterial solution of Bifidobacterium longum CICC 25033 at an inoculum amount of 2-5%, mixing and fermenting in a cylinder, the anaerobic fermentation is carried out at a temperature of 36-37° C. for 10 to 24 hours; then adding the bacterial solution of Lactobacillus plantarum CICC 6053 at an inoculum amount of 2-5% of the total mass of Rhizoma Dioscoreae Bulbiferae and Rhizoma Coptis chinensis, carrying out aerobic fermentation at a temperature of 37° C. for 10 to 24 hours, and after the fermentation is completed, adding 3% hydrogen peroxide to inactivate Bifidobacterium longum and Lactobacillus plantarum CICC 6053 to obtain a fermented product; S5, take the fermented product obtained in S3 and add 70% ethanol 10 times the amount, reflux extraction for 30 minutes, repeat 3 times, discard the residue, combine the filtrate, recover the alcohol, and concentrate until there is no alcohol taste, and obtain the fermented product extract for use; S6. Take Centella asiatica, add 10 times the amount of 70% ethanol, reflux extraction for 30 minutes, repeat 3 times, discard the residue, combine the filtrate, recover the alcohol, and concentrate until there is no alcohol taste, and obtain the Centella asiatica extract for use; S7, the Chinese medicinal materials except Dioscorea bulbifera, Coptis chinensis and Centella asiatica in step S2 are mixed evenly, 10 times the amount of water is added, and the mixture is decocted for 40 min, and the filtrate is filtered and taken, and the mixture is repeated 3 times, and the filtrates are combined and concentrated to a relative density of 1.1-1.2 (60° C.) to obtain a Chinese medicinal concentrate; S8. Heat the vaseline to melt and keep it warm, then add the fermentation extract obtained in step S5, the Centella asiatica extract obtained in step S6 and the Chinese medicine concentrate obtained in step S7 while stirring, continue stirring until it becomes viscous, let it cool naturally, then add the ground borneol while stirring, and mix thoroughly to obtain a plaster.

6. The method for preparing the repairing cream for repairing various scars according to claim 5, characterized in that: The preparation concentration was 3.0×10 8 cfu / mL of Bifidobacterium longum CICC 25033 and a concentration of 3.6×10 8 cfu / mL Lactobacillus plantarum CICC6053 bacterial liquid.

7. The method for preparing the repairing cream for repairing various scars according to claim 5, characterized in that: The inoculation amount of the bacterial solution of Bifidobacterium longum CICC 25033 and the bacterial solution of Lactobacillus plantarum CICC 6053 was 3% of the total weight of Dioscorea bulbifera and Coptis chinensis.

8. The method for preparing the repairing cream for repairing various scars according to claim 5, characterized in that: In step S6, vaseline is heated to 80°C for melting and heat preservation, and then the medicinal oil is added while stirring, and then medical zinc oxide powder is added while stirring, and it is allowed to cool naturally. After cooling to a temperature of 60°C, borneol is added while stirring, and the plaster is obtained after being fully mixed.

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