Bactericidal composition for preventing and treating bitter gourd wilt

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition for preventing and treating bitter gourd wilt. The invention relates to a bactericidal composition for preventing and treating bitter gourd wilt. The active ingredients of the bactericidal composition are compounded from fluindazole amine and carvacrol or oxathiapiprolin. A compounding strategy is adopted, the fluindazole amine and the carvacrol or the oxathiapiprolin are compounded, an indoor biological activity test shows that the compounded combination has a synergistic effect on bitter gourd wilt germs, compared with a single component, the prevention and control effect on bitter gourd wilt can be improved, then the bitter gourd wilt is effectively prevented and controlled, and the pesticide composition has a good application prospect. A support can be provided for developing a novel medicament for preventing and treating bitter gourd fusarium wilt.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition for preventing and treating bitter melon wilt disease. Background Art

[0002] Bitter melon is a vine plant of the genus Momordica in the Cucurbitaceae family. It is a vegetable crop used for both medicine and food. It is deeply loved by people because of its jade-like clearness, crisp taste and rich nutrition. Due to the deterioration of the soil environment for planting bitter melons due to continuous cropping, the continuous cropping obstacles are becoming increasingly serious, and the occurrence of soil-borne diseases has become more serious. Bitter melon wilt caused by Fusarium oxysporumSchl.f.sp.momordicae is a soil-borne disease that seriously affects the yield and quality of bitter melon. Its general incidence rate is 15-25%, and the incidence rate can reach 60% in plots with continuous cropping obstacles. When it is prevalent, a large number of yellowed and dead plants appear in the field, resulting in a decrease in the yield of bitter melon and causing serious economic losses.

[0003] So far, chemical agents are still the most effective and direct means of preventing and controlling bitter melon wilt. The main chemical agents used in production are methyl thiophanate, carbendazim, tebuconazole and thiophanate-methyl. However, due to the fast growth and high adaptability of bitter melon wilt pathogens, continuous use of the same chemical agent can easily make the pathogen resistant to pesticides. Therefore, it is of great value to screen and develop new agents for preventing and controlling bitter melon wilt.

[0004] Fluindapyr is a pyrazoleamide fungicide and a succinate dehydrogenase inhibitor fungicide. It inhibits the function of mitochondria by interfering with the tricarboxylic acid cycle on complex II of the respiratory electron transport chain, preventing them from producing energy, inhibiting the growth of pathogens, and ultimately causing their death. It can be used to prevent and control a variety of plant fungal diseases.

[0005] There are no reports on the combination of fluoxathiapiprolin with carvacrol or fluthiazolin.

[0006] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0007] The purpose of the present invention is to provide a bactericidal composition for preventing and treating bitter melon wilt disease, so as to solve the problem.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] A bactericidal composition for preventing and treating bitter melon wilt disease, wherein the active component is prepared by compounding fluoxathiapiprolin with carvacrol or fluthiazolin.

[0010] Preferably, the composite mass ratio of fluinoxadone to carvacrol is 1-10:40-1.

[0011] Preferably, the compounding mass ratio of fluoxathiapiprolin and trifloxystrobin is 1-16:16-1.

[0012] More specifically, the pathogen of the bitter melon wilt disease is Fusarium oxysporum var. balsam pear.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] (1) The present invention adopts a compounding strategy to compound fluoxathiapiprolin with carvacrol or fluthiazolin. Indoor biological activity tests have shown that the compound combination exhibits a synergistic effect on bitter melon wilt pathogens. Compared with a single component, the compound combination can improve the control effect on bitter melon wilt disease, thereby effectively preventing and controlling bitter melon wilt disease, and can provide support for the development of new agents for preventing and controlling bitter melon wilt disease.

[0015] (2) Compared with a single chemical agent, the bactericidal composition of the present invention can reduce the risk of drug resistance, slow down the development of pathogens, and reduce the cost of prevention and treatment. DETAILED DESCRIPTION

[0016] The technical solution of the present invention is clearly and completely described below in conjunction with specific embodiments.

[0017] 1. Indoor biological activity test

[0018] 1.1 Test strains

[0019] The stem bases of plants infected with bitter melon wilt were collected and isolated and cultured using a conventional tissue separation method. The pathogen was identified as Fusarium oxysporum Schl.f.sp. momordicae.

[0020] 1.2 Test agents

[0021] 97% fluoxathiapiprolin technical drug, 92.5% carvacrol technical drug, 95% fluthiazolin technical drug, all of which are commercially available.

[0022] Each of the above test agents was dissolved in dimethyl sulfoxide and then diluted with a 0.1% Tween-80 aqueous solution to prepare a single-dose stock solution with an active ingredient concentration of 10,000 mg / L. Multiple groups of proportions were set up, and each single-dose stock solution and the proportioned mixture were diluted with a 0.1% Tween-80 aqueous solution to 5 mass concentration gradients for standby use.

[0023] 1.3 Test methods

[0024] The mycelium growth rate method (Mu Liyi 1994) was used. The drug solution and the culture medium at about 50°C were mixed evenly in a triangular flask at a volume ratio of 1:9, and poured into a sterile culture dish with a diameter of 9 cm to prepare drug-containing plates containing different masses of pesticide solution, 10 mL per dish, and 0.1% Tween-80 aqueous solution was used as a blank control. Each treatment was repeated 5 times, and each repeated 1 plate.

[0025] Inoculate the test strain on PDA medium and culture at 28±1℃ for 4 days. Use a hole puncher to punch a 5mm diameter bacterial cake along the edge of the colony. After the culture medium solidifies, place a bacterial cake in the center of the drug-containing plate and the blank control. Cover the dish with a lid and place it in an incubator. After culturing at 28±1℃ for 5 days, measure the colony diameter using the cross method and calculate the mycelium growth inhibition rate of different treatments.

[0026]

[0027] 1.4 Data Analysis

[0028] DPS software was used for data statistical analysis. The logarithmic value of fungicide concentration was taken as x and the corresponding probability value of mycelium growth inhibition rate was taken as y for linear regression to calculate the toxicity EC of the agent to the pathogen. 50 The co-toxicity coefficient (CTC) was calculated using the Sun Yunpei method.

[0029]

[0030] In the above formula: ATI--the toxicity index of the mixture; S--the EC of the standard agent 50 , the unit is mg / L; M--EC of the mixture 50 , unit is mg / L.

[0031] TTI=TI A ×P A +TI B ×P B

[0032] In the above formula: TTI-theoretical toxicity index of the mixture; TI A --Toxicity index of A agent; P A --The percentage of agent A in the mixture, in percentage (%); TI B --Toxicity index of agent B; P B --The percentage of agent B in the mixture, in percentage (%).

[0033]

[0034] In the above formula: CTC--co-toxicity coefficient; ATI--actual toxicity index of the mixture; TTI--theoretical toxicity index of the mixture.

[0035] 1.5 Results Analysis

[0036] The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC). CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 1-2.

[0037] Table 1 Indoor bioactivity test of flutoxacillin and carvacrol against Fusarium wilt of bitter melon

[0038] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Fluoxathiapiprolin 1.3527 100.0000 -- -- Carvacrol 11.2479 12.0262 -- -- Fluoxathiapiprolin 1:Carvacrol 40 5.2270 25.8791 14.1719 182.6079 Fluoxathiapiprolin 1:Carvacrol 30 3.3628 40.2254 14.8641 270.6211 Fluoxathiapiprolin 1:Carvacrol 20 2.1114 64.0665 16.2155 395.0949 Fluoxathiapiprolin 1:Carvacrol 10 4.6752 28.9335 20.0239 144.4952 Fluoxathiapiprolin 1: Carvacrol 5 3.9206 34.5024 26.6885 129.2779 Fluoxathiapiprolin 1: Carvacrol 1 1.7428 77.6165 56.0131 138.5684 Fluoxathiapiprolin 5: Carvacrol 1 1.0372 130.4184 85.3377 152.8263 Fluoxathiapiprolin 10: Carvacrol 1 0.9155 147.7553 92.0024 160.5994

[0039] As shown in Table 1, after flufenacet and carvacrol were compounded, the co-toxicity coefficients against Fusarium wilt pathogenic bacteria of bitter melon were all greater than 120 in the mass ratio range of 1-10:40-1, showing a synergistic effect.

[0040] Table 2 Indoor bioactivity determination of fluoxathiapiprolin and fluthiazolinone against Fusarium wilt pathogen of bitter melon

[0041]

[0042]

[0043] As shown in Table 2, after fluoxathiapiprolin and fluthiazolin are compounded, the co-toxicity coefficients against Fusarium wilt pathogenic bacteria of bitter melon are all greater than 120 in the mass ratio range of 1-16:16-1, showing a synergistic effect.

[0044] In summary, the present invention adopts a compounding strategy to compound fluoxathiapiprolin with carvacrol or fluthiazolin. Indoor biological activity tests have shown that the compound combination shows a synergistic effect on bitter melon wilt pathogens. Compared with a single ingredient, the compound combination can improve the control effect on bitter melon wilt disease, thereby effectively preventing and controlling bitter melon wilt disease, and can provide support for the development of new agents for preventing and controlling bitter melon wilt disease.

[0045] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A bactericidal composition for preventing and treating bitter melon wilt, characterized in that: Its active ingredients are a combination of fluoxetine and carvacrol or fluthiazolin.

2. The bactericidal composition for preventing and treating bitter melon wilt according to claim 1, characterized in that: The composite mass ratio of fluinoxadone to carvacrol is 1-10:40-1.

3. The bactericidal composition for preventing and treating bitter melon wilt according to claim 1, characterized in that: The compounding mass ratio of fluoxathiapiprolin and flubendiamide is 1-16:16-1.

4. The bactericidal composition for preventing and treating bitter melon wilt according to claim 1, characterized in that: The pathogen of the bitter melon wilt disease is the bitter melon-specific type of Fusarium oxysporum.

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