A composition for improving skin oiliness and stress-induced hair loss and use thereof

Abstract

The present application belongs to the technical field of cosmetics, and particularly relates to a composition for improving skin oil secretion and stress-induced alopecia and application thereof. The composition comprises the following components in mass fractions: lavender essential oil 0.001-0.15 parts, and usnea extract 1.0-5.0 parts. It is verified by experiments that the composition can synergistically activate ectopic olfactory receptors in the skin and hair follicles, synergistically reduce cortisol secretion, and synergistically improve the problems of skin oil secretion and stress-induced alopecia, and therefore can be used in skin care cosmetics and hair cosmetics, and has good practical application value.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a composition for improving skin oiliness and stress-induced hair loss and application thereof. BACKGROUND

[0002] The information disclosed in this Background section is only for the purpose of increasing the understanding of the general background of the application and does not necessarily constitute an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.

[0003] Psychological stress can cause complex neuroendocrine responses, mainly including the increase of sympathetic adrenal medulla system (SAM) and hypothalamic-pituitary-adrenal axis (HPA axis) activities. Cortisol is an important stress hormone in HPA axis, commonly known as "stress hormone". Under normal circumstances, the level of cortisol is regulated by the internal biological clock system, and stress can significantly disturb the cortisol level and the shock curve, causing abnormal increase of cortisol, and further affecting the function of sebaceous glands, the immune function of skin, the skin barrier function, the skin color, etc. Studies have shown that high levels of cortisol can increase the activity of 5α-reductase, promote the conversion of testosterone to more potent dihydrotestosterone, increase the synthesis of skin oil, and lead to increased skin oiliness.

[0004] In addition to the effects on the skin, long-term mental stress can also have adverse effects on hair growth. Normally, hair grows, falls off, and regrows in a cycle related to the growth cycle of hair follicles. During the growth phase, hair follicle stem cells are activated and differentiate into hair matrix cells and inner root sheath cells, resulting in the production of new hair; then the hair follicle enters the regression phase and the hair stops growing; then the hair follicle enters the resting phase, and after a period of time, the hair falls off. After the resting phase, the hair follicle reenters the growth phase and new hair grows. Cortisol can directly act on hair follicle stem cells to inhibit their proliferation and differentiation, leading to premature entry of growing hair into the resting phase and falling off. An article published in Nature magazine shows that for experimental mice, their adrenal glands also release a substance similar to cortisol, namely corticosterone, when they are under stress. When the corticosterone level of mice is elevated, the resting phase of many hair follicles is prolonged, resulting in delayed growth of new hair. Conversely, if the adrenal glands are removed and corticosterone cannot be secreted, the resting hair follicles will quickly enter the next growth phase, and the active hair follicles involved in hair growth are about 3 times that of the corticosterone group. These results show that high levels of corticosterone for a long time affect the activation of hair follicle stem cells and inhibit the growth of new hair. The main means for treating hair loss is the use of minoxidil, which mainly targets androgenic alopecia, and has only a certain auxiliary effect on the treatment of stress-induced alopecia. During its use, it may also cause skin irritation, such as itching, redness, or burning of the scalp, and temporary exacerbation of hair loss within a short period of time after use. These problems have made the public maintain a wait-and-see attitude towards it.

[0005] In 1991, scientists discovered a class of GPCRs receptors that can be activated by specific odor molecules, known as olfactory receptors (ORs). To date, more than 300 functional ORs have been discovered, and ORs are widely expressed in non-olfactory tissues in addition to the nasal cavity. Non-olfactory tissues cannot distinguish odors, but can be triggered by odor molecules, and are known as ectopic olfactory receptors (EORs). Studies have shown that EORs are expressed in skin tissue cells such as keratinocytes and melanocytes, and are involved in cell proliferation, pigment synthesis, wound healing, and hair growth.

[0006] In the prior art, patent application CN116459186A provides an anti-aging essential oil composition for relieving emotions, which can not only relieve emotions but also has anti-aging effects, and can reduce the cortisol content in human saliva, but does not have the effects of improving skin oiliness and stress-induced alopecia.

[0007] Studies have shown that ectopic olfactory receptor OR2AT4 is distributed in keratinocytes and the epithelium of hair follicles (especially the outer root sheath), and synthesizes sandalwood odorants The OR2AT4 receptor can be activated to produce a calcium signal, activate downstream signaling pathways, promote the proliferation and migration of keratinocytes, promote epidermal re-epithelialization, and promote hair growth; the skin cells also contain OR1A1 receptors, which can activate downstream signaling pathways through the cAMP / PKA / CREB signaling axis after activation, wherein phosphorylated CREB activates HES1, inhibits the expression of PPAR-gamma, and further inhibits the expression of lipid synthesis-related genes, resulting in a decrease in the content of triglycerides, cholesterol, and free fatty acids. In the prior art, there is no research report on regulating skin oiliness by activating OR1A1 receptors. In the prior art, synthetic sandalwood odorants can specifically activate OR2AT4 in hair follicles, prolong the in-vitro growth of human hair by reducing cell apoptosis and increasing the production of growth factor IGF-1. Patent application CN115666728 A provides a preparation method of sandalwood extract and application of the composition of the consumed sandalwood extract in cosmetics for skin, scalp and appendages. The sandalwood extract can activate ectopic olfactory receptors OR2AT4 in skin and hair follicles, improve the epidermal barrier, brighten the skin, promote IGF-1 expression, and be active in maintaining hair growth. However, it does not have the effects of improving skin oiliness and stress-induced hair loss.

[0008] Patent application CN116899262 A provides a preparation method of Usma grass composite extract, which comprises peach kernel, Usma grass leaves, Usma grass seeds, lavender flowers, cultivated black seed, myrtle and ginger roots, and has the effect of preventing hair loss. However, the patent application does not prove any hair loss prevention effect, and does not have the effects of improving skin oiliness, regulating ectopic olfactory receptors and reducing cortisol secretion. It can be seen that there are still few cosmetic raw materials / compositions that can regulate ectopic olfactory receptors, reduce cortisol secretion, and improve skin oiliness and stress-induced hair loss at present, and the market urgently needs raw materials / compositions that can safely and effectively solve the problem. SUMMARY

[0009] In view of the deficiencies in the prior art, the present application aims to provide a composition for improving skin oiliness and stress-induced hair loss and its application. The composition comprises lavender essential oil and Usma grass extract, which has been verified by experiments that it can synergistically activate ectopic olfactory receptors in skin and hair follicles, synergistically reduce cortisol secretion, and synergistically improve skin oiliness and stress-induced hair loss. Based on the above research results, the present application is completed.

[0010] In order to achieve the above technical purpose, the technical scheme provided by the present application is as follows:

[0011] In a first aspect of the present application, a composition for improving skin oiliness and stress-induced hair loss is provided, which comprises the following components in mass fractions:

[0012] Lavender essential oil 0.001-0.15 parts, Usma grass extract 1.0-5.0 parts.

[0013] In the present application, the lavender essential oil can be obtained by commercial methods or according to the prior art, which is not specifically limited here.

[0014] The Usma grass extract is prepared by extracting Usma grass, according to the Uighur Pharmacopoeia: Usma grass is a biennial herb of the Brassicaceae Chamaesyce genus. It contains effective components such as chamaesyce, sinigrin, indole alcohol, and indigo red. It is cool in nature, and has the functions of clearing heat and resolving toxins, and removing black bile. Further, the Usma grass extract is obtained by the following preparation method: mixing Usma grass with an ethanol solution, extracting the extract by hot reflux, concentrating the extract, then adding anhydrous ethanol, and refrigerating to obtain the supernatant. The supernatant is filtered, concentrated, then water is added and filtered to obtain the water-soluble extract, and then a preservative and water and butanediol are added to obtain the Usma grass extract.

[0015] In a second aspect of the present application, the use of the above-mentioned composition in the preparation of a product for improving skin oiliness and stress-induced hair loss is provided.

[0016] The product can be a cosmetic product, which can further be a skin care cosmetic product and / or a hair care cosmetic product.

[0017] In a third aspect of the present application, a cosmetic product for improving skin oiliness and stress-induced hair loss is provided, which comprises the above-mentioned composition; the cosmetic product can be a skin care cosmetic product and / or a hair care cosmetic product.

[0018] In a fourth aspect of the present application, a preparation method of the above-mentioned cosmetic product for improving skin oiliness and stress-induced hair loss is provided, which comprises the step of mixing the composition and other raw material components.

[0019] The above-mentioned one or more technical solutions have the following beneficial technical effects:

[0020] The technical solution provides a safe solution for improving skin oil secretion and stress-induced alopecia without dependence. The composition composed of lavender essential oil and usnea extract can: 1) synergistically activate the mRNA expression of ectopic olfactory receptor OR1A1 in human immortalized keratinocytes HaCaT, and relieve the influence of stress on the skin state; 2) synergistically activate the mRNA expression of ectopic olfactory receptor OR2AT4 in human hair follicle keratinocytes, improve the influence of stress on hair growth, and improve stress-induced alopecia; 3) synergistically reduce the secretion of cortisol, and then improve the skin problems and alopecia problems caused by stress; 4) synergistically reduce the oil content of human skin, and play an oil control role. Based on the above technical effects, the composition can synergistically improve the problems of skin oil secretion and stress-induced alopecia.

[0021] Meanwhile, the cosmetic preparation method obtained by the technical solution is simple and easy to implement, raw materials are easy to obtain, and is suitable for mass production, and therefore has good practical application value. BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required to be used in the embodiment description will be briefly introduced. Obviously, the drawings in the following description are only embodiments of the present application, and other drawings can be obtained by the drawings provided by those skilled in the art without creative labor.

[0023] Figure 1 The results of the influence of Examples 1 to 4 and Comparative Examples 1 to 16 of the present application on the mRNA expression amount of ectopic olfactory receptor OR1A1 of human immortalized keratinocytes HaCaT are shown in the following figure:

[0024] Figure 2 The results of the influence of Examples 1 to 4 and Comparative Examples 1 to 16 of the present application on the mRNA expression amount of ectopic olfactory receptor OR2AT4 of human hair follicle keratinocytes are shown in the following figure:

[0025] Figure 3 The results of the influence of Examples 1 to 4 and Comparative Examples 1 to 2 of the present application on stress-induced alopecia in mice are shown in the following figure:

[0026] Among them, Figure 1 and Figure 2In the table, the significance is indicated by #, 0.01 DETAILED DESCRIPTION

[0027] It should be noted that the following detailed description is illustrative only, and is intended to provide further description in order to provide a fuller enabling teaching of the present application. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the application pertains.

[0028] It is also important to note that the terms "comprises", "comprising", "includes", "including", "contains", "containing" or variations thereof herein, do not specify an exhaustive or complete list of components or features as used by those skilled in the art. Unless otherwise expressed, the terms "comprises", "comprising", "includes", "including", "contains", "containing" or variations thereof herein, are intended to be inclusive or open-ended and not exclude additional, unrecited components, features, steps, devices, assemblies or variations thereof.

[0029] In one exemplary embodiment of the present application, a composition for improving skin oiliness and stress-induced hair loss is provided, which comprises the following components in the following mass fractions:

[0030] Lavender essential oil 0.001-0.15 parts, Usnea extract 1.0-5.0 parts.

[0031] In the present application, the lavender essential oil can be obtained by commercial means or prepared according to the prior art, which is not specifically limited herein.

[0032] The Usnea extract is obtained by the following preparation method: mixing Usnea with an ethanol solution, obtaining an extract by hot reflux extraction, concentrating the extract, then adding anhydrous ethanol, and obtaining the supernatant by refrigeration, filtering the supernatant, concentrating, then adding water and standing, filtering to obtain the water-sediment, adding a preservative and water and butylene glycol to obtain the Usnea extract.

[0033] In another exemplary embodiment of the present application, the preparation method of the Usnea extract comprises:

[0034] S1: mixing Usma grass with 20%~25% (volume fraction) ethanol solution at a solid-liquid ratio of 1:10~1:15 (g / mL), refluxing at 50~100 ℃ for 0.5 h~2 h, filtering at 80 mesh, separating the Usma grass powder from the extract, and obtaining a first extract;

[0035] S2: mixing the Usma grass obtained in step S1 with 20%~25% ethanol solution at a solid-liquid ratio of 1:5~1:10 (g / mL), refluxing at 50~100 ℃ for 0.5 h~2 h, filtering, separating the Usma grass powder from the extract, and obtaining a second extract;

[0036] S3: combining the first extract and the second extract, and concentrating to 0.5-2 times (preferably 1 time) the weight of the Usma grass in step S1 to obtain a concentrated solution;

[0037] S4: adding the concentrated solution obtained in step S3 into anhydrous ethanol to obtain an ethanol content of 40-60% (preferably 50%) in the system, and placing at low temperature for 12 h or more to obtain a supernatant;

[0038] S5: filtering the supernatant obtained in step S4 to obtain a filtrate;

[0039] S6: concentrating the filtrate obtained in step S5 under reduced pressure to 0.1-0.5 times (preferably 0.3 times) the weight of the Usma grass in step S1, then adding 0.5-1 times (preferably 0.7 times) the weight of water of the Usma grass, placing at low temperature for 12 h or more, and then filtering to obtain an aqueous precipitate;

[0040] S7: adding 0.1-1% (preferably 0.9%) of phenoxyethanol and 0.05-0.2% (preferably 0.1%) of ethylhexylglycerin as preservatives into the aqueous precipitate obtained in step S6, and then adding 0.1-1 times (preferably 0.4 times) the weight of water of the Usma grass and 0.2-0.6 times (preferably 0.6 times) the weight of butanediol, and mixing uniformly to obtain the Usma grass extract.

[0041] In another specific embodiment of the present application, in step S1, the volume fraction of the ethanol solution is 25%, the solid-liquid ratio is 1:13, the extraction time is 1.5 h, and the extraction temperature is 90~100 ℃; the filtering is performed by sieve filtering, and the sieve mesh is 60-100 mesh, preferably 80 mesh.

[0042] In step S2, the volume fraction of the ethanol solution is 25%, the solid-liquid ratio is 1:8, the extraction time is 1 h, and the extraction temperature is 90~100 ℃; the filtering is performed by sieve filtering, and the sieve mesh is 60-100 mesh, preferably 80 mesh.

[0043] In steps S4 and S6, the temperature for placing at low temperature can be 4 ℃±2 ℃.

[0044] In the step S5, the filtration can be performed using a 0.45 μm filter plate.

[0045] In the step S6, the filtration can be performed using a 0.45 μm filter plate and a 0.45 μm polypropylene film (PP film).

[0046] In another specific embodiment of the present application, the use of the above-mentioned composition in the preparation of a product for improving skin oiliness and stress-induced hair loss is provided.

[0047] The product can be a cosmetic product. It should be noted that, in the present application, the cosmetic product refers to a daily-use chemical industrial product that is spread on any part of the human body surface (skin, hair, nails, lips, etc.) by rubbing, spraying or other similar methods to achieve the purposes of cleaning, eliminating bad odor, skin care, beautifying and modifying. As can be seen from the above, the composition of the present application has the effect of improving skin oiliness and stress-induced hair loss, and thus the cosmetic product can be a skin care cosmetic product and a hair care cosmetic product.

[0048] Therefore, in another specific embodiment of the present application, a cosmetic product for improving skin oiliness and stress-induced hair loss is provided, wherein the cosmetic product comprises the above-mentioned composition; and the cosmetic product can be a skin care cosmetic product and a hair care cosmetic product.

[0049] In another specific embodiment of the present application, in the cosmetic product, the final concentration of the lavender essential oil is 0.001-0.15 wt%, and the final concentration of the Usnea extract is 1.0-5.0 wt%.

[0050] The cosmetic product can further comprise other raw material components allowed to be added in the field of cosmetics, including but not limited to emulsifiers, emollients, humectants and thickening agents, etc.

[0051] Meanwhile, the present application can also be used to prepare different cosmetic dosage forms, such as liquids, gels, creams, ointments, lotions, sprays, masks or lyophilized powders, etc., by reasonably adding the above-mentioned raw material components, without being specifically limited herein.

[0052] In another specific embodiment of the present application, a preparation method of the above-mentioned cosmetic product for improving skin oiliness and stress-induced hair loss is provided, wherein the preparation method comprises the step of mixing the composition and other raw material components.

[0053] The application is further illustrated below with examples. The application is further illustrated below by way of examples, but is not therefore limited in scope to the examples described. Any variation to the application based on the examples in the application falls within the scope of protection of the application without being creative. The lavender essential oil used in the examples and comparative examples was purchased from Xinjiang Yiparkhan Spice Co., Ltd.; other reagents and materials were commercially available unless otherwise specified.

[0054] The preparation method of the Usnea extract described in Examples 1-4 is as follows:

[0055] S1: Usnea was mixed with 25% ethanol at a solid-liquid ratio (g / mL) of 1:13, and refluxed at 90-100°C for 1.5h. The Usnea was separated from the extract by 80-mesh filtration to obtain a first extract;

[0056] S2: The Usnea obtained in step S1 was mixed with 25% ethanol at a solid-liquid ratio (g / mL) of 1:8, and refluxed at 90-100°C for 1h. The Usnea was separated from the extract by 80-mesh filtration to obtain a second extract;

[0057] S3: The first extract and the second extract were combined and concentrated to 1 times the weight of the Usnea to obtain a concentrated solution;

[0058] S4: The concentrated solution obtained in step S3 was added with anhydrous ethanol to a system ethanol content of 50%, and stored at 4°C±2°C for 12h or more to retain the supernatant.

[0059] S5: The supernatant obtained in step S4 was filtered with a 0.45μm filter plate to obtain a filtrate.

[0060] S6: The filtrate obtained in step S5 was concentrated under reduced pressure to 0.3 times the weight of the Usnea, then 0.7 times the weight of the Usnea was added with water, and stored at 4°C±2°C for 12h or more, then filtered with a 0.45μm filter plate and a 0.45μm PP membrane to obtain a water precipitate;

[0061] S7: To the water precipitate obtained in step S6, 0.9% of phenoxyethanol and 0.1% of ethylhexylglycerin were added as preservatives, then 0.4 times the weight of the Usnea was added with water and 0.6 times the weight of the Usnea was added with butylene glycol, and mixed uniformly to obtain the Usnea extract.

[0062] In addition, in the comparative examples, the Usnea extract was added by the same method as in Examples 1-4 unless otherwise specified.

[0063] Examples:

[0064] Examples 1-4 provide a composition that can synergistically activate ectopic olfactory receptors in skin and hair follicles, synergistically reduce cortisol secretion, synergistically improve skin oiliness and stress-induced hair loss problems, and the ingredient ratio is shown in Table 1. As a comparison, Table 1 also provides Comparative Examples 1-16, which Comparative Examples 1-8 differ from the examples in that they only contain one of the substances in the composition. Comparative Examples 9, 11-13 differ from Examples and Comparative Examples 2 / 6 / 7 / 8 in that they only contain the Usnea extract in the composition, and the preparation method of the Usnea extract is different. Comparative Examples 10, 14-16 differ from Example 1 in that the Usnea extract in the composition is the Usnea extract prepared in Comparative Examples 9, 11-13, respectively.

[0065] wherein:

[0066] The preparation method of the Usnea extract in Comparative Examples 9 and 10 is the same as in Patent Application CN116899262A.

[0067] The specific preparation process of the Usnea extract in Comparative Examples 11 and 14 is as follows:

[0068] S1: Mix Usnea with 25% ethanol at a solid-liquid ratio (g / mL) of 1:9, reflux extract at 90-100°C for 1.5h, filter at 80 mesh, separate the Usnea from the extract, and obtain a first extract;

[0069] S2: Mix the Usnea obtained in step S1 with 25% ethanol at a solid-liquid ratio (g / mL) of 1:4, reflux extract at 90-100°C for 1h, filter at 80 mesh, separate the Usnea from the extract, and obtain a second extract;

[0070] S3: Combine the first extract and the second extract, concentrate to 1 times the weight of Usnea, and obtain a concentrated solution;

[0071] S4: Add anhydrous ethanol to the concentrated solution obtained in step S3 to a system ethanol content of 50%, store at 4°C±2°C for 12h or more, and retain the supernatant.

[0072] S5: Filter the supernatant obtained in step S4 using a 0.45μm filter plate to obtain a filtrate.

[0073] S6: Concentrate the filtrate obtained in step S5 under reduced pressure to 0.3 times the weight of Usnea, then add 0.7 times the weight of Usnea of water, store at 4°C±2°C for 12h or more, then filter using a 0.45μm filter plate and a 0.45μm PP membrane to obtain a water-sedimented solution;

[0074] S7: To the water sediment obtained in step S6, 0.9% of phenoxyethanol and 0.1% of ethylhexylglycerin as preservatives were added, and 0.4 times the weight of water and 0.6 times the weight of butylene glycol were added, and mixed uniformly to obtain the extract of Usnea.

[0075] The specific preparation process of the Usnea extract in Comparative Examples 12 and 15 is as follows:

[0076] S1: The Usnea was mixed with 75% ethanol at a solid-liquid ratio (g / mL) of 1:13, and reflux extraction was performed at 90-100°C for 1.5h. The Usnea was separated from the extract by 80-mesh filtration to obtain a first extract;

[0077] S2: The Usnea obtained in step S1 was mixed with 75% ethanol at a solid-liquid ratio (g / mL) of 1:8, and reflux extraction was performed at 90-100°C for 1h. The Usnea was separated from the extract by 80-mesh filtration to obtain a second extract;

[0078] S3: The first extract and the second extract were combined and concentrated to 1 times the weight of the Usnea to obtain a concentrated solution;

[0079] S4: The concentrated solution obtained in step S3 was added with anhydrous ethanol to a system ethanol content of 50%, and was stored at 4°C±2°C for more than 12h to reserve the supernatant.

[0080] S5: The supernatant obtained in step S4 was filtered with a 0.45μm filter plate to obtain a filtrate.

[0081] S6: The filtrate obtained in step S5 was concentrated under reduced pressure to 0.3 times the weight of the Usnea, and then 0.7 times the weight of water was added. The mixture was stored at 4°C±2°C for more than 12h, and then filtered with a 0.45μm filter plate and a 0.45μm PP membrane to obtain a water sediment;

[0082] S7: To the water sediment obtained in step S6, 0.9% of phenoxyethanol and 0.1% of ethylhexylglycerin as preservatives were added, and 0.4 times the weight of water and 0.6 times the weight of butylene glycol were added, and mixed uniformly to obtain the extract of Usnea.

[0083] The specific preparation process of the Usnea extract in Comparative Examples 13 and 16 is as follows:

[0084] S1: The Usnea was mixed with 25% ethanol at a solid-liquid ratio (g / mL) of 1:13, and reflux extraction was performed at 90-100°C for 3h. The Usnea was separated from the extract by 80-mesh filtration to obtain a first extract;

[0085] S2: The Usma grass obtained in step S1 was mixed with 25% ethanol at a solid-liquid ratio of 1:8 (g / mL), and refluxed at 90-100°C for 3 h. The Usma grass was separated from the extract by 80-mesh filtration to obtain a secondary extract;

[0086] S3: The primary extract and the secondary extract were combined and concentrated to 1 times the weight of the Usma grass to obtain a concentrated solution;

[0087] S4: The concentrated solution obtained in step S3 was added with anhydrous ethanol to a system ethanol content of 50%, and stored at 4°C±2°C for 12 h or more, and the supernatant was reserved.

[0088] S5: The supernatant obtained in step S4 was filtered with a 0.45 μm filter plate to obtain a filtrate.

[0089] S6: The filtrate obtained in step S5 was concentrated under reduced pressure to 0.3 times the weight of the Usma grass, then 0.7 times the weight of the Usma grass of water was added, and stored at 4°C±2°C for 12 h or more, and then filtered with a 0.45 μm filter plate and a 0.45 μm PP membrane to obtain a water precipitate;

[0090] S7: To the water precipitate obtained in step S6, 0.9% of phenoxyethanol and 0.1% of ethylhexylglycerin were added as preservatives, and then 0.4 times the weight of the Usma grass of water and 0.6 times the weight of the Usma grass of butylene glycol were added and mixed uniformly to obtain an Usma grass extract.

[0091] Table 1. Raw material ingredients and proportions of compositions of Examples 1-4 and Comparative Examples 1-16

[0092]

[0093]

[0094] A composition composed of lavender essential oil and Usma grass extract was added to a double-layer spray base to prepare a skin care product having the effects of reducing skin oil secretion and saliva cortisol content, i.e., Example 5, while Comparative Example 17 and Comparative Example 18 were set up. The difference between Comparative Example 17 and Comparative Example 18 and Example 5 is that Comparative Example 17 and Comparative Example 18 contain only lavender essential oil or Usma grass extract. The specific raw material ingredients and proportions are shown in Table 2, and the effects of improving skin oil secretion and reducing saliva cortisol content were tested through human efficacy experiments.

[0095] Table 2. Raw material ingredients and proportions of double-layer spray of Example 5 and Comparative Examples 17 and 18

[0096]

[0097] The products of Example 5, Comparative Example 17 and Comparative Example 18 are prepared by using the conventional method in the art, and need to be shaken to mix before use.

[0098] A composition consisting of lavender essential oil and usnea extract is added to a shampoo base to prepare a shampoo having the effect of improving stress-induced hair loss, i.e. Example 6, while a placebo group is set up, i.e. Comparative Example 19, which differs from Example 6 in that the composition consisting of lavender essential oil and usnea extract is not contained in Comparative Example 19. The specific raw material ingredients and proportions are shown in Table 3.

[0099] Table 3. Shampoo raw material ingredients and proportions of Example 6 and Comparative Example 19

[0100]

[0101]

[0102] The products of Example 6 and Comparative Example 19 are prepared by using the conventional method in the art.

[0103] Experimental Example:

[0104] The products of the above examples and comparative examples are subjected to performance testing, which is as follows:

[0105] Experimental material preparation:

[0106] The mouse data used in the following tests is from Qilu University of Technology; human hair follicle keratinocytes are from Shanghai Jingang Biological Engineering Co., Ltd.; human immortal keratinocytes HaCaT are from the Shanghai Cell Bank of the Chinese Academy of Sciences; other reagents and materials are ordinary commercially available products unless otherwise specified.

[0107] I. Effects of Examples 1-4 and Comparative Examples 1-16 on the expression of ectopic olfactory receptor OR1A1 mRNA in keratinocytes HaCaT

[0108] 1. Experimental method: Human immortal keratinocytes HaCaT in good growth state were collected and a cell suspension was prepared with complete culture solution, and the cell concentration was adjusted to 1.3 x 10 5The cells were seeded at a density of 1.5 x 105cells / mL, 2 mL / well of cell suspension was added to a 6-well cell culture plate. The blank control group (high-sugar DMEM culture solution), the negative control group (10 nM cortisol + high-sugar DMEM culture solution), and the sample group (10 nM cortisol + high-sugar DMEM culture solution containing the compositions of Examples 1-4 or Comparative Examples 1-16) were set up in triplicate. The cells were incubated in a cell incubator (5% CO2, 37°C) for 24 h, and the cell confluence reached 40-60%. The complete culture solution was discarded. The blank control group was added with high-sugar DMEM culture solution, and the rest of the groups were added with high-sugar DMEM culture solution containing 10 nM cortisol and incubated in a cell incubator for 12 h. Then the liquid in the plate was discarded, and the cells were washed twice with PBS buffer. The blank control group and the negative control group were added with high-sugar DMEM culture solution, and the sample group was added with high-sugar DMEM culture solution containing the compositions of Examples 1-4 or Comparative Examples 1-16 and incubated in a cell incubator for 12 h. The culture solution was discarded, the cells were collected, and RNA was extracted. The relative expression of ectopic olfactory receptor OR1A1 mRNA was detected by RT-qPCR.

[0109] The test concentration of the composition samples of Examples 1-4 and Comparative Examples 1-16 is shown in Table 1, and the solvent is high-sugar DMEM culture solution containing 0.1% (v / v) DMSO. For the synergistic effect of the composition, the Chou-Talalay formula is used for determination:

[0110] Q = E(a+b) / (Ea+Eb-Ea x Eb);

[0111] Wherein Q < 0.55 is obvious antagonism, Q = 0.55-0.85 is antagonism, Q = 0.85-1.15 is simple addition, and Q > 1.15 is synergistic effect.

[0112] 2. Experimental results: as shown in Table 2, the compositions of Examples 1-4 have synergistic effect on the expression of ectopic olfactory receptor OR1A1 mRNA. Figure 1As shown in Table 4, after cortisol stimulation, the relative expression of ectopic olfactory receptor OR1A1 mRNA in the negative control group was significantly lower than that in the blank control group (P<0.001), indicating that stress can affect the expression of ectopic olfactory receptors in skin keratinocytes; compared with the negative control group, after cortisol stimulation, the addition of the compositions of Examples 1-4 and Comparative Examples 1-16 to the keratinocyte stress model, except for Comparative Examples 2, 6, 7, 8, 9, 11, 12, and 13, the relative expression of ectopic olfactory receptor OR1A1 mRNA was significantly increased (P<0.05). Among them, the relative expression of ectopic olfactory receptor OR1A1 mRNA in Examples 1-4 was increased by 113.62%, 103.96%, 110.34%, and 60.53%, respectively, and the relative expression of ectopic olfactory receptor OR1A1 mRNA in Comparative Examples 1-16 was increased by 38.99%, -0.40%, 43.46%, 51.75%, 32.32%, -1.17%, 3.17%, 9.45%, 1.01%, 40.88%, -5.95%, -7.87%, -1.49%, 36.68%, 32.33%, and 38.95%, respectively. Analysis shows that the use of lavender essential oil alone in Comparative Examples 1, 3, 4, and 5 has a certain effect on increasing the relative expression of ectopic olfactory receptor OR1A1 mRNA in keratinocytes, and the use of Usnea extract alone in Comparative Examples 2, 6, 7, and 8 shows low-concentration inhibition and high-concentration slight increase in the relative expression of ectopic olfactory receptor OR1A1 mRNA in keratinocytes, but there is no significant difference. The use of Usnea extract prepared by a different process from Examples alone in Comparative Examples 9, 11, 12, and 13 has no effect on increasing the relative expression of ectopic olfactory receptor OR1A1 mRNA in keratinocytes. Compared with the results of Examples 1-4, it can be seen that the use of the two in combination significantly increases the effect; taking Example 1 as an example, E(a+b)=113.62%, Ea=38.99%, and Eb=-0.40%, and Q=2.93>1.15 is obtained by substituting into the King's formula, showing synergistic effect; similarly, the lavender essential oil and Usnea compositions of Examples 2-4 all achieve synergistic effect in increasing the relative expression of ectopic olfactory receptor OR1A1 mRNA in keratinocytes. The E(a+b) of Comparative Example 10 is 40.88%, Ea is 38.99%, Eb is 1.01%, and Q=1.03<1.15 is obtained by substituting into the King's formula, which is simply the effect of the two substances added together and has no synergistic effect. Similarly, Q=1.04<1.15 for Comparative Example 14, Q=0.94<1.15 for Comparative Example 15, and Q=1.02<1.15 for Comparative Example 16, all of which are simply the effect of the two substances added together and have no synergistic effect. The above results show that the Usnea extract prepared by the process of Examples 1-4 and the lavender essential oil have a synergistic effect.

[0113] Table 4. Effects of Examples 1-4 and Comparative Examples 1-16 on the expression of ectopic olfactory receptor OR1A1 mRNA in keratinocytes HaCaT

[0114]

[0115]

[0116] II. Effects of Examples 1-4 and Comparative Examples 1-16 on the expression of ectopic olfactory receptor OR2AT4 mRNA in human hair follicle keratinocytes

[0117] 1. Experimental method: Collect human hair follicle keratinocytes in good growth state and prepare a cell suspension with EliteCell primary keratinocyte complete culture medium, adjust the cell concentration to 1.0 x 10 5 6-well cell culture plates. The blank control group (EliteCell primary keratinocyte complete culture medium), the negative control group (10 nM cortisol + EliteCell primary keratinocyte complete culture medium), and the sample group (10 nM cortisol + EliteCell primary keratinocyte complete culture medium containing Examples 1-4 or Comparative Examples 1-16) were set up in triplicate. Incubate in a cell incubator (5% CO2, 37°C) for 24 h, and the cell confluence rate reaches 40-60%. Discard the EliteCell primary keratinocyte complete culture medium. Add EliteCell primary keratinocyte complete culture medium to the blank control group, and add EliteCell primary keratinocyte complete culture medium containing 10 nM cortisol to the other groups, and incubate in a cell incubator for 12 h. Then discard the liquid in the well plate, wash twice with PBS buffer, add EliteCell primary keratinocyte complete culture medium to the blank control group and the negative control group, and add EliteCell primary keratinocyte complete culture medium containing Examples 1-4 or Comparative Examples 1-16 to the sample group, and incubate in a cell incubator for 12 h. Discard the culture medium, collect the cells, extract RNA, and detect the relative expression of ectopic olfactory receptor OR2AT4 mRNA by RT-qPCR.

[0118] The test concentrations of Examples 1-4 and Comparative Examples 1-16 are shown in Table 1, and the solvent is EliteCell primary keratinocyte complete culture medium containing 0.1% (v / v) DMSO. For the synergistic effect of the composition, the King's formula is used for determination:

[0119] Q = E(a+b) / (Ea+Eb-Ea x Eb);

[0120] Q < 0.55 indicates significant antagonism, Q = 0.55–0.85 indicates antagonism, Q = 0.85–1.15 indicates simple addition, and Q > 1.15 indicates synergistic effect.

[0121] 2. Experimental results: such as Figure 2 As shown in Table 5, after cortisol stimulation, the relative expression level of ectopic olfactory receptor OR2AT4 mRNA in human hair follicle keratinocytes of the negative control group was significantly lower than that of the blank control group (P < 0.01), indicating that stress affects the expression of ectopic olfactory receptors in human hair follicle keratinocytes. Compared with the negative control group, after cortisol stimulation, the relative expression level of ectopic olfactory receptor OR2AT4 mRNA was significantly increased after adding the compositions of Examples 1-4 and Comparative Examples 1-16 to the human hair follicle keratinocyte stress model (P < 0.05). Among them, the relative expression increases of ectopic olfactory receptor OR2AT4 mRNA in Examples 1-4 were 113.34%, 96.06%, 105.44%, and 70.64%, respectively, while the relative expression increases of ectopic olfactory receptor OR2AT4 mRNA in Comparative Examples 1-16 were 33.75%, 25.53%, 47.59%, 66.47%, 26.95%, 20.08%, 35.11%, 44.04%, 18.47%, 45.39%, 18.72%, 17.91%, 23.50%, 49.22%, 40.03%, and 52.36%, respectively. Analysis shows that using lavender essential oil or osmanthus alone can increase the relative expression of ectopic olfactory receptor OR2AT4 mRNA in human hair follicle keratinocytes. However, compared with the results of Examples 1-4, it can be seen that the combined use of the two significantly increases the enhancement effect. Taking Example 1 as an example, E(a+b) = 113.34%, Ea = 33.75%, Eb = 25.53%, and substituting into the King's Law formula, we get Q = 2.24 > 1.15, showing a synergistic effect. Similarly, the lavender essential oil and eusmarin combination in Examples 2-4 both achieved a synergistic effect in increasing the relative expression level of ectopic olfactory receptor OR2AT4 mRNA in human hair follicle keratinocytes, which can improve the effect of stress on hair growth. In Comparative Example 10, E(a+b) = 45.39%, Ea = 33.75%, Eb = 18.47%, and substituting into the King's Law formula, we get Q = 0.99 < 1.15, which is simply the sum of the effects of the two substances, without any synergistic effect. Similarly, Q = 1.07 < 1.15 for Comparative Example 14, Q = 0.88 < 1.15 for Comparative Example 15, and Q = 1.06 < 1.15 for Comparative Example 16, all of which are simply the sum of the effects of the two substances, without any synergistic effect. These results indicate that the *Usma oleracea* extract and lavender essential oil processed in Examples 1-4 have a synergistic effect.

[0122] Table 5. Effects of Examples 1-4 and Comparative Examples 1-16 on the expression of OR2AT4 in human hair follicle keratinocytes

[0123]

[0124]

[0125] III. Effects of Examples 1-4 and Comparative Examples 1-2 on mouse stress alopecia

[0126] 1. Experimental method: Preparation of corticosterone solution: dissolved using physiological saline containing 0.1% DMSO and 0.1% Tween-80, with a final concentration of 4 mg / mL of corticosterone;

[0127] Examples 1-4 and Comparative Examples 1-2 solution: dissolved using physiological saline containing 0.1% DMSO, with a final concentration of Examples 1-4 and Comparative Examples 1-2 compositions as shown in Table 1;

[0128] The mice were raised in the animal house of Qilu University of Technology, all female mice, body weight 18-20 g, the experiment set up a blank control group (injected with 0.1% DMSO and 0.1% Tween-80 containing physiological saline + smearing 0.1% DMSO containing physiological saline), negative control group (injected with corticosterone solution + smearing 0.1% DMSO containing physiological saline) and sample group (injected with corticosterone solution + smearing Examples 1-4 / Comparative Examples 1-2 solution), 6 in each group, light environment 12h-dark environment 12h, alternating. The temperature was kept at 22℃±1℃, and the humidity was 30%-70%.

[0129] After 3 days of adaptive feeding, the mouse back hair was shaved short with a small animal shaver, and the remaining hair was removed by applying depilatory cream. When the mouse skin was pink and had no damage, it was confirmed that the hair follicles were in the resting phase. The negative control group and the sample group were injected with 20 mg / kg of corticosterone solution in the mouse back depilation area, 1 time / day, and the blank control group was injected with the same volume of 0.1% DMSO and 0.1% Tween-80 containing physiological saline, and the modeling was continuously injected for 3 weeks. The sample group began to smear Examples 1-4 and Comparative Examples 1-2 solution after 1 week of modeling, 1 time / day, 50 μL each time, continuously for 4 weeks, and the blank control group and the negative control group were smeared with the same volume of 0.1% DMSO containing physiological saline, and the hair growth was compared by taking pictures at the 4th week.

[0130] 2. Experimental results: After smearing the samples for 4 weeks, the mouse back depilation area was photographed for observation. The experimental results are as follows Figure 3As shown, the back hair of the blank control group mice has completely recovered without any exposed skin, the back hair of the negative control group mice has not recovered with a large amount of exposed skin, and the back hair of the mice in the example 1 to 4 and comparative example 1 to 2 groups has grown to different degrees. From the analysis of the hair growth amount, the comparative example 1 and comparative example 2 have a certain effect of promoting hair growth, the example 1 to 4 have a better effect of promoting hair growth than the comparative example 1 to 2, and no adverse reactions and repeated problems after stopping are observed, and have a synergistic effect.

[0131] Four, the influence of example 5 and comparative examples 17 and 18 on the oil content of human skin

[0132] 1. Selection and requirements of volunteers

[0133] 90 male volunteers who have been in a high-pressure state for a long time and have oily skin are selected, and the oil content of the skin on the forehead is ≥120 μg / cm 2 , and the age is 18 to 35 years old, which are divided into three groups (example 5 group, comparative example 17 group and comparative example 18 group), each group has 30 people, all the test subjects have no history of skin or systemic diseases, the test site is normal, and no other drugs or cosmetics are applied during the test period.

[0134] 2. Test environment

[0135] The test site is constant temperature and humidity, the environmental temperature is 20 to 22℃, the relative humidity is 40% to 60%, and the test subjects should be in a stable state before the test. After the test subjects wash their faces with water at about 35℃, they sit still for 30 minutes in the test environment before starting the test.

[0136] 3. Sample usage method

[0137] The test samples are the product of example 5, the product of comparative example 17 and the product of comparative example 18. The matrix of comparative example 17 and comparative example 18 is consistent with that of example 5, and the difference is that comparative example 17 and comparative example 18 only contain one of lavender essential oil or usma grass extract. Each is used once a day, morning and evening after cleansing, for 28 consecutive days.

[0138] 4. Test items

[0139] (1) Experimental method: Sebumeter (SM815, Courage and Khazaka, Germany) was used to test the skin oil content after 0 days and 28 days of product use. The Sebumeter method is a world-recognized method for testing skin oil content. The skin oil content tester measures the change in light transmission of a special 0.1 mm thick extinction tape before and after absorbing oil to represent the skin oil content. In the test, the tape is in contact with the test area, and after absorbing the oil on the human skin, it becomes translucent, and the light transmission also changes. The more oil absorbed, the greater the light transmission, and the light transmission of the tape can measure the skin oil content.

[0140] Cortisol content test: At the same time point, the cortisol content in the saliva of 90 volunteers after 0 days and 28 days of product use was detected by high-sensitivity enzyme immunoassay kit (ELISA). The volunteers gargled 30 minutes before the test and did not eat or drink water within 30 minutes. Then the cotton swab in the saliva collection tube was placed under the tongue for about 5 minutes. After the cotton swab was completely soaked, it was put back into the tube and centrifuged at 3000 r / min for 15 minutes. The liquid was collected for cortisol content detection.

[0141] For the synergistic effect of the composition, the King's formula was used for judgment:

[0142] Q = E(a+b) / (Ea+Eb-Ea x Eb);

[0143] Where Q <0.55 is obvious antagonism, Q = 0.55-0.85 is antagonism, Q = 0.85-1.15 is simple addition, and Q > 1.15 is synergistic effect.

[0144] (2) Experimental results: as shown in Table 6, after 28 days of using the test sample, the oil content of the Example 5 group decreased by 29.49%, the oil content of the Comparative Example 17 group decreased by 2.21%, and the oil content of the Comparative Example 18 group decreased by 4.02%, and no adverse reactions occurred; according to the Kim formula, E(a+b) = 29.49%, Ea = 2.21%, and Eb = 4.02%, and Q = 4.80 > 1.15 is obtained by substituting into the Kim formula, proving that the composition containing lavender essential oil and usnea extract can synergistically reduce the oil content of the skin and has an oil control effect; after 28 days of using the test sample, the cortisol content in the saliva of the Example 5 group decreased by 19.32%, the cortisol content in the saliva of the Comparative Example 17 group decreased by 2.21%, and the cortisol content in the saliva of the Comparative Example 18 group decreased by 5.04%; according to the Kim formula, E(a+b) = 19.32%, Ea = 2.21%, and Eb = 5.04%, and Q = 2.71 > 1.15 is obtained by substituting into the Kim formula, proving that the composition containing lavender essential oil and usnea extract can synergistically reduce the cortisol content in the saliva and reduce stress.

[0145] Table 6. Results of the effects of Example 5, Comparative Example 17, and Comparative Example 18 on the oil content of human skin and the cortisol content in saliva

[0146]

[0147] Note: P < 0.05 is considered to have a significant difference compared with Comparative Example 17 and Comparative Example 18.

[0148] The above results show that the composition containing lavender essential oil and usnea extract can synergistically activate ectopic olfactory receptors in the skin and hair follicles, synergistically reduce the secretion of cortisol, and synergistically improve the problems of skin oiliness and stress-induced hair loss.

[0149] It should be noted that the above examples are only used to illustrate the technical solutions of the present application but not to limit it. Although the present application is explained in detail with reference to the examples given, the technical solutions of the present application can be modified or replaced by equivalents according to the needs of those skilled in the art without departing from the spirit and scope of the technical solutions of the present application.

Claims

1. A composition for improving skin oiliness and stress-induced hair loss, characterized by, The composition comprises components in the following mass fractions: lavender essential oil 0.001-0.15 parts, usnea extract 1.0-5.0 parts; The usnea extract is obtained by the following preparation method: S1: mixing usnea and 20%-25% ethanol solution at a solid-liquid ratio of 1:10-1:15 g / mL, refluxing at 50-100°C for 0.5-2 h, filtering through a 80-mesh sieve, separating the usnea powder from the extract, and obtaining a first extract; S2: mixing the usnea obtained in step S1 and 20%-25% ethanol solution at a solid-liquid ratio of 1:5-1:10 g / mL, refluxing at 50-100°C for 0.5-2 h, filtering, separating the usnea powder from the extract, and obtaining a second extract; concentrating the extract, then adding anhydrous ethanol, and refrigerating to obtain supernatant; filtering the supernatant, concentrating, then adding water, standing, filtering to obtain water sediment, adding a preservative and water and butanediol to the water sediment, and obtaining the usnea extract.

2. The composition of claim 1, wherein The preparation method of the usnea extract comprises: S1: mixing usnea and 20%-25% ethanol solution at a solid-liquid ratio of 1:10-1:15 g / mL, refluxing at 50-100°C for 0.5-2 h, filtering through a 80-mesh sieve, separating the usnea powder from the extract, and obtaining a first extract; S2: mixing the usnea obtained in step S1 and 20%-25% ethanol solution at a solid-liquid ratio of 1:5-1:10 g / mL, refluxing at 50-100°C for 0.5-2 h, filtering, separating the usnea powder from the extract, and obtaining a second extract; S3: combining the first extract and the second extract, concentrating to 0.5-2 times the weight of the usnea in step S1, and obtaining a concentrated solution; S4: adding the concentrated solution obtained in step S3 to anhydrous ethanol to obtain an ethanol content of 40-60% in the system, and standing at low temperature for 12 h or more to obtain supernatant; S5: filtering the supernatant obtained in step S4 to obtain a filtrate; S6: concentrating the filtrate obtained in step S5 under reduced pressure to 0.1-0.5 times the weight of the usnea in step S1, then adding 0.5-1 times the weight of the usnea in water, standing at low temperature for 12 h or more, then filtering to obtain water sediment; S7: adding 0.1-1% phenoxyethanol and 0.05-0.2% ethylhexylglycerin as preservatives to the water sediment obtained in step S6, then adding 0.1-1 times the weight of the usnea in water and 0.2-0.6 times the weight of the usnea in butanediol, and uniformly mixing to obtain the usnea extract.

3. The composition of claim 2, wherein In step S1, the volume fraction of the ethanol solution is 25%, the solid-liquid ratio is 1:13, the extraction time is 1.5 h, and the extraction temperature is 90-100°C; the filtering is sieve filtering, and the sieve mesh size is 80 mesh.

4. The composition of claim 2, wherein In step S2, the volume fraction of the ethanol solution is 25%, the solid-liquid ratio is 1:8, the extraction time is 1 h, and the extraction temperature is 90-100°C; the filtering is sieve filtering, and the sieve mesh size is 80 mesh.

5. The composition of claim 2, wherein In steps S4 and S6, the low-temperature standing temperature is 4°C±2°C.

6. The composition of claim 2, wherein In the step S5, the filtration is performed using a 0.45 μm filter plate. In the step S6, the filtration is performed using a 0.45 μm filter plate and a 0.45 μm polypropylene film.

7. Use of the composition according to any one of claims 1 to 6 for the manufacture of a product for improving skin oiliness and stress-induced hair loss.

8. The use according to claim 7, characterized in that, The product is a cosmetic product.

9. The use according to claim 8, wherein the compound is ###0002### The product is a skin care cosmetic product and / or a hair care cosmetic product.

10. A cosmetic for improving skin oiliness and stress-induced hair loss, characterized by, The cosmetic product comprises the composition according to any one of claims 1 to 6.

11. The cosmetic product of claim 10, wherein the cosmetic product is a skin care product. The cosmetic product is a skin care cosmetic product and / or a hair care cosmetic product.

12. The method for preparing the cosmetic for improving sebum excretion and stress-induced alopecia according to claim 10 or 11, characterized in that, The production method comprises a step of mixing the composition and other raw material components.

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