Compound plant composition with soothing and repairing effects, preparation method and application thereof

Abstract

The present invention relates to the technical field of skin care product raw materials, and specifically discloses a compound plant composition with soothing and repairing effects, a preparation method and an application. The preparation method of the compound plant composition with soothing and repairing effects comprises the following steps: step 1), putting the composite raw material into water, soaking for 6-8 hours, and obtaining a mixture; step 2), adding composite bacteria into the mixture, fermenting for 10-12 days, and obtaining a fermentation product; step 3), filtering, sterilizing, and concentrating the fermentation product to obtain a compound plant composition with soothing and repairing effects; the composite raw material is a compound of peony root, medicinal pore fungus, and scutellaria root; the composite bacteria is a compound of tuchetti sausage-associated lactobacillus, Jadinseberlindner yeast, and Bacillus clausii. The present invention has the advantage of improving the soothing effect of skin care products.

Description

Technical Field

[0001] The present invention relates to the field of skin care product raw materials, and in particular to a compound plant composition with soothing and repairing effects, a preparation method and an application thereof. Background Art

[0002] The discomfort caused by skin sensitivity and inflammation may cause the shedding of keratinocytes in severe cases, affecting the normal differentiation of dermal cell collagen and other problems, which greatly affects people's quality of life. The improvement of image brought by cosmetics often brings a certain burden to the skin, because base makeup products basically cannot do without adding a large amount of mineral powder and synthetic pigments, etc. Ultra-fine mineral powder may clog pores, and daily makeup and makeup removal also make the skin's stratum corneum more and more fragile; in addition, with the improvement of material and spiritual life, frequent changes in eating habits and life rhythms are also the causes of skin sensitivity and inflammation; in some special cosmetics, such as whitening and sunscreen products, although the main functional ingredients added can play a certain role, these chemical ingredients often cause allergies, and formulation engineers have gradually realized the seriousness of this problem. In the development stage of such cosmetics, adding anti-allergic and anti-inflammatory ingredients has become the primary factor that must be considered.

[0003] Research has found that some plant extracts have anti-allergic and anti-inflammatory effects. Adding the extracts to skin care products can have a certain anti-inflammatory and soothing effect. However, the existing extracts are relatively general in soothing effect and it is difficult to have a significant soothing effect. The effect of repairing the skin is slow to take effect and it is difficult to meet the needs of consumers. Therefore, there is still room for improvement. Summary of the invention

[0004] In order to improve the soothing effect of skin care products, the present application provides a compound plant composition with soothing and repairing effects, a preparation method and an application thereof.

[0005] In the first aspect, the present application provides a method for preparing a compound plant composition with soothing and repairing effects, using the following technical solution:

[0006] A method for preparing a compound plant composition with soothing and repairing effects comprises the following steps:

[0007] Step 1), putting the composite raw material into water and soaking for 6-8 hours to obtain a mixture;

[0008] Step 2), adding composite bacteria to the mixture and fermenting for 10-12 days to obtain a fermentation product;

[0009] Step 3), filtering, sterilizing and concentrating the fermented product to obtain a compound plant composition with soothing and repairing effects;

[0010] The composite raw material is a compound of peony root, medicinal pore fungus, and scutellaria root;

[0011] The composite bacteria is a combination of Lactobacillus associated with Tucchetti sausage, Saccharomyces cerevisiae and Bacillus clausii.

[0012] By adopting the above technical scheme, by specifically selecting peony root, medicinal pore fungus, and scutellaria root for compounding, and then using a composite bacteria composed of Lactobacillus tuceti, Saccharomyces cerevisiae, and Bacillus clausii for fermentation, the content of active ingredients in the extract is high, and the active ingredients and the fermentation products can cooperate with each other to exert very significant anti-inflammatory and soothing effects, making the soothing effect very obvious, and can quickly repair the skin, effectively improve allergic skin, and restore the skin to health.

[0013] Preferably, in the composite raw material, the mass ratio of peony root, medicinal pore fungus and scutellaria root is 1-3:1-3:0.3-0.7.

[0014] By adopting the above technical scheme and specifically selecting the mass ratio of peony root, medicinal pore fungus and scutellaria root, the active ingredients can cooperate with each other better and have more significant anti-inflammatory and soothing effects.

[0015] Preferably, in the composite bacteria, the mass ratio of Lactobacillus tucheti sausage-associated, Saccharomyces cerevisiae Jendingeberlindner's yeast, and Bacillus clausii is 1-2:1-2:1-2.

[0016] By adopting the above technical scheme and specifically selecting the mass ratio of Lactobacillus tucheti sausage-associated, Saccharomyces cerevisiae Jendingeberlindner's yeast and Bacillus clausii, the fermentation effect is better, the concentration of the active ingredients is better increased, and the effect of the fermentation product and the active ingredients cooperating with each other is better, thereby better improving the anti-inflammatory and soothing effects.

[0017] Preferably, the mass ratio of the composite raw material to water is 2.3-6.7:20-30.

[0018] By adopting the above technical solution and specifically selecting the mass ratio of the composite raw material to water, the extraction effect is better, and the energy consumption of the subsequent concentration step can be reduced, which has a higher economic value.

[0019] Preferably, the mass ratio of the composite raw material to the composite bacteria is 2.3-6.7:0.003-0.006.

[0020] By adopting the above technical scheme and specifically selecting the mass ratio of the composite raw materials and the composite bacteria, the fermentation effect is better, and at the same time, the active ingredients and the fermentation products cooperate better with each other, and the anti-inflammatory and soothing effects are better.

[0021] Preferably, in the step 3), the mass ratio of the compound plant composition with soothing and repairing effects obtained after concentration to the composite raw material added in the step 1) is 2.3-6.7:10.

[0022] By adopting the above technical solution, the concentration of active ingredients and fermentation products in the concentrated compound plant composition with soothing and repairing effects is just right, which makes it more convenient to add to skin care products later, reduces the difficulty of the process, and is better adapted to the formula of skin care products.

[0023] Preferably, in step 2), the temperature is kept at 32-34° C. and the fermentation is carried out for 10-12 days.

[0024] By adopting the above technical solution and keeping the temperature at 32-34°C, the activity of the composite bacteria is higher, the fermentation effect is better, the fermentation efficiency is higher, and the economic value is higher.

[0025] In the second aspect, the present application provides a compound plant composition with soothing and repairing effects, which adopts the following technical solution:

[0026] A compound plant composition with soothing and repairing effects is prepared by the preparation method of the compound plant composition with soothing and repairing effects.

[0027] By adopting the above technical scheme, the prepared compound plant composition with soothing and repairing effects can play the current anti-inflammatory and soothing effects, can quickly repair the skin, quickly improve sensitive skin, make the skin less likely to recur allergies, and reduce skin discomfort.

[0028] In a third aspect, the present application provides an application of a compound plant composition having soothing and repairing effects, using the following technical solution:

[0029] An application of the above-mentioned compound plant composition with soothing and repairing effects, wherein the compound plant composition with soothing and repairing effects is added to skin care products to prepare skin care products with anti-inflammatory, soothing and repairing effects.

[0030] By adopting the above technical solution, the prepared skin care products can have better anti-allergic and soothing effects while retaining other effects of the skin care products, and can effectively relieve skin irritation caused by other ingredients in the skin care products, so that the skin can be better repaired in all aspects and the skin can be restored to health.

[0031] In summary, this application has the following beneficial effects:

[0032] 1. Since the present application specifically selects peony root, medicinal pore fungus, and scutellaria root for compounding and then uses a composite bacteria composed of Lactobacillus tuceti sausage-associated, Saccharomyces cerevisiae, and Bacillus clausii for fermentation, the content of active ingredients in the extract is relatively high, and the active ingredients and fermentation products can cooperate with each other to exert very significant anti-inflammatory and soothing effects, making the soothing effect very obvious, and can quickly repair the skin, effectively improve allergic skin, and restore the skin to health.

[0033] 2. In the present application, it is preferred to specifically select the mass ratio of peony root, medicinal pore fungus, and scutellaria root so that the active ingredients cooperate with each other better and have more significant anti-inflammatory and soothing effects.

[0034] 3. In the present application, it is preferred to specifically select the mass ratio of Lactobacillus tucheti sausage-associated, Saccharomyces cerevisiae Jendinger, and Bacillus clausii to achieve better fermentation effect, better increase the concentration of active ingredients, and make the fermentation product and the active ingredients cooperate better, thereby better improving the anti-inflammatory and soothing effects. DETAILED DESCRIPTION

[0035] The present application is further described in detail below with reference to the embodiments.

[0036] Example 1

[0037] A compound plant composition with soothing and repairing effects, and a preparation method of the compound plant composition with soothing and repairing effects comprises the following steps:

[0038] Step 1), put 1kg of peony root, 1kg of medicinal pore fungus, and 0.3kg of scutellaria root into 20kg of water and soak for 6 hours to obtain a mixture.

[0039] Step 2), add 1g of Lactobacillus associated with Tucchetti sausage, 1g of Saccharomyces cerevisiae and 1g of Bacillus clausii into the mixture, keep the mixture at 32°C, and ferment for 12 days to obtain a fermentation product.

[0040] Step 3), the fermented product is filtered through a 200-mesh filter cloth to remove the filter residue to obtain a filtrate, the filtrate is filtered through a 0.22 μm filter membrane for sterilization to obtain a sterilized solution, the sterilized solution is concentrated by rotary evaporation, the rotary evaporation pressure is 0.01 MPa, and the rotary evaporation temperature is 50°C to obtain 10 kg of a compound plant composition with soothing and repairing effects.

[0041] The peony root was purchased from Huamei Medicinal Materials Store in Juancheng County and is a dried Chinese medicinal material.

[0042] The medicinal phyllotoxin was purchased from Daxinganling Senyuanshan Products Co., Ltd. and is a dried Chinese medicinal material.

[0043] The root of Scutellaria baicalensis was purchased from Anhui Kangweifu Pharmaceutical Co., Ltd. as a dry Chinese medicinal material.

[0044] The Lactobacillus associated with Tucchetti sausage was purchased from Beijing Biobo Biotechnology Co., Ltd., platform number: Bio-84625.

[0045] Yeast of Jardine Seiberlin was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-52752.

[0046] Bacillus clausii was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-05392.

[0047] Example 2

[0048] A compound plant composition with soothing and repairing effects, and a preparation method of the compound plant composition with soothing and repairing effects comprises the following steps:

[0049] Step 1), put 2kg of peony root, 2kg of medicinal pore fungus, and 0.5kg of scutellaria root into 25kg of water, and soak for 7h to obtain a mixture.

[0050] Step 2), 1.5 g of Lactobacillus associated with Tucchetti sausage, 1.5 g of Saccharomyces cerevisiae and 1.5 g of Bacillus clausii were added to the mixture, and the mixture was kept at a constant temperature of 33° C. and fermented for 11 days to obtain a fermentation product.

[0051] Step 3), the fermented product is filtered through a 200-mesh filter cloth to remove the filter residue to obtain a filtrate, the filtrate is filtered through a 0.22 μm filter membrane for sterilization to obtain a sterilized solution, the sterilized solution is concentrated by rotary evaporation, the rotary evaporation pressure is 0.01 MPa, and the rotary evaporation temperature is 50°C to obtain 10 kg of a compound plant composition with soothing and repairing effects.

[0052] The peony root was purchased from Huamei Medicinal Materials Store in Juancheng County and is a dried Chinese medicinal material.

[0053] The medicinal phyllotoxin was purchased from Daxinganling Senyuanshan Products Co., Ltd. and is a dried Chinese medicinal material.

[0054] The root of Scutellaria baicalensis was purchased from Anhui Kangweifu Pharmaceutical Co., Ltd. as a dry Chinese medicinal material.

[0055] The Lactobacillus associated with Tucchetti sausage was purchased from Beijing Biobo Biotechnology Co., Ltd., platform number: Bio-84625.

[0056] Yeast of Jardine Seiberlin was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-52752.

[0057] Bacillus clausii was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-05392.

[0058] Example 3

[0059] A compound plant composition with soothing and repairing effects, and a preparation method of the compound plant composition with soothing and repairing effects comprises the following steps:

[0060] Step 1), 3 kg of peony root, 3 kg of medicinal pore fungus, and 0.7 kg of scutellaria root are put into 30 kg of water and soaked for 8 hours to obtain a mixture.

[0061] Step 2), 2g of Lactobacillus associated with Tucchetti sausage, 2g of Saccharomyces cerevisiae and 2g of Bacillus clausii were added to the mixture, and the mixture was kept at a constant temperature of 34°C and fermented for 10 days to obtain a fermentation product.

[0062] Step 3), the fermented product is filtered through a 200-mesh filter cloth to remove the filter residue to obtain a filtrate, the filtrate is filtered through a 0.22 μm filter membrane for sterilization to obtain a sterilized solution, the sterilized solution is concentrated by rotary evaporation, the rotary evaporation pressure is 0.01 MPa, and the rotary evaporation temperature is 50°C to obtain 10 kg of a compound plant composition with soothing and repairing effects.

[0063] The peony root was purchased from Huamei Medicinal Materials Store in Juancheng County and is a dried Chinese medicinal material.

[0064] The medicinal phyllotoxin was purchased from Daxinganling Senyuanshan Products Co., Ltd. and is a dried Chinese medicinal material.

[0065] The root of Scutellaria baicalensis was purchased from Anhui Kangweifu Pharmaceutical Co., Ltd. as a dry Chinese medicinal material.

[0066] The Lactobacillus associated with Tucchetti sausage was purchased from Beijing Biobo Biotechnology Co., Ltd., platform number: Bio-84625.

[0067] Yeast of Jardine Seiberlin was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-52752.

[0068] Bacillus clausii was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-05392.

[0069] Comparative Example 1

[0070] A compound plant composition with soothing and repairing effects, compared with Example 2, the only difference is:

[0071] Replace peony root with an equal amount of Centella asiatica.

[0072] Centella asiatica comes from commercially available, dried Chinese medicinal materials.

[0073] Comparative Example 2

[0074] A compound plant composition with soothing and repairing effects, compared with Example 2, the only difference is:

[0075] An equal amount of licorice was used to replace the medicinal pore fungus.

[0076] Licorice is derived from commercially available, dry Chinese medicinal materials.

[0077] Comparative Example 3

[0078] A compound plant composition with soothing and repairing effects, compared with Example 2, the only difference is:

[0079] Replace the skullcap root with an equal amount of lavender.

[0080] Lavender comes from commercially available, dried Chinese medicinal materials.

[0081] Comparative Example 4

[0082] A compound plant composition with soothing and repairing effects, compared with Example 2, the only difference is:

[0083] An equal amount of Centella asiatica was used to replace the peony root, an equal amount of licorice was used to replace the medicinal pore fungus, and an equal amount of lavender was used to replace the scutellaria root.

[0084] Centella asiatica comes from commercially available, dried Chinese medicinal materials.

[0085] Licorice is derived from commercially available, dry Chinese medicinal materials.

[0086] Lavender comes from commercially available, dried Chinese medicinal materials.

[0087] Comparative Example 5

[0088] A compound plant composition with soothing and repairing effects, compared with Example 2, the only difference is:

[0089] The palm fermentation Bacillus was used to replace the Lactobacillus associated with Tucchetti sausage in equal amounts.

[0090] Bacillus palmitolyticus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-00034.

[0091] Comparative Example 6

[0092] A compound plant composition with soothing and repairing effects, compared with Example 2, the only difference is:

[0093] Kluyveromyces marxianus was used to replace Saccharomyces jadinseberlindnerii in equal amounts.

[0094] Kluyveromyces marxianus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-67356.

[0095] Comparative Example 7

[0096] A compound plant composition with soothing and repairing effects, compared with Example 2, the only difference is:

[0097] Bacillus megaterium was used to replace Bacillus clausii in equal amounts.

[0098] Bacillus megaterium was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-67364.

[0099] Comparative Example 8

[0100] A compound plant composition with soothing and repairing effects, compared with Example 2, the only difference is:

[0101] The palm fermentation Bacillus was used to replace the Lactobacillus associated with Tucchetti sausage in equal amounts, the Kluyveromyces marxianus was used to replace the Jedinseiberlindner yeast in equal amounts, and the Bacillus megaterium was used to replace the Bacillus clausii in equal amounts.

[0102] Bacillus palmitolyticus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-00034.

[0103] Kluyveromyces marxianus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-67356.

[0104] Bacillus megaterium was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-67364.

[0105] Experiment 1

[0106] According to T / JSRH 003-2023 "Hyaluronidase inhibition method for testing the anti-allergic activity of cosmetic raw materials", the hyaluronidase inhibition rate (%) of the test samples prepared from the compound plant composition with soothing and repairing effects of each embodiment and comparative example was tested.

[0107] Sample to be tested: Take 5 g of the compound plant composition with soothing and repairing effects and 95 g of deionized water, mix them evenly to obtain the sample to be tested.

[0108] Experiment 2

[0109] The inflammatory factor inhibition effect of the test samples prepared from the compound plant compositions with soothing and repairing effects of the embodiments and comparative examples was detected.

[0110] Sample to be tested: Take 5 g of the compound plant composition with soothing and repairing effects and 95 g of deionized water, mix them evenly to obtain the sample to be tested.

[0111] Experimental steps:

[0112] (1) Cell seeding: dilute macrophages (mouse mononuclear macrophage leukemia cell line RAW264.7, purchased from the Cell Bank of the Chinese Academy of Sciences, catalog number: TCM13) with cell culture medium to the seeding density (the degree of confluence reaches 45%-60% 24 h after seeding), seed them into 96-well plates with 200 μL per well, and place them in a CO2 incubator for 24±2 h.

[0113] (2) Administration: Discard the culture medium in the 96-well plate and carry out induction and administration. Add culture medium containing a certain concentration of the test substance and 1 μg / mL LPS to the test substance wells (using the test sample), add cell culture medium containing LPS to the negative control wells, and add cell culture medium to the blank control wells, 200 μL per well, and continue to culture in a CO2 incubator for 24±2 h.

[0114] (3) Collection of cell supernatant: After the incubation, collect 200 μL of cell culture supernatant into a 1.5 mL sterile centrifuge tube and store it in a -80°C ultra-low temperature freezer.

[0115] (4) ELISA test: Perform the test according to the operating instructions of the TNF-α ELISA test kit.

[0116] The experimental results of Experiment 1-2 are shown in Table 1.

[0117] Table 1

[0118]

[0119] According to the data comparison of each embodiment and comparative example in Table 1, the hyaluronidase inhibition rate and TNF-α inhibition rate of each embodiment are significantly higher than those of each comparative example. It can be seen that although comparative examples 1-4 use other plant raw materials with soothing effects to replace the peony root, medicinal pore fungus, and scutellaria root in the embodiments alone or in whole, they can have a soothing effect, but the soothing effect is far lower than that of each embodiment.

[0120] In comparative examples 5-8, other fermentation bacteria species are used to replace the Lactobacillus tucheti, Saccharomyces cerevisiae, and Bacillus clausii in the examples alone or in whole. Although they can also exert the good soothing and anti-inflammatory effects of the combination of peony root, medicinal pore fungus, and scutellaria root, the coordination system of the fermentation product and the active ingredient is destroyed, resulting in the soothing and anti-inflammatory effects that are better than those of comparative examples 1-4, but are still significantly lower than those of the examples.

[0121] It is proved that only when peony root, medicinal pore fungus, and scutellaria root are compounded and fermented by Lactobacillus tuceti, Saccharomyces cerevisiae, and Bacillus clausii, the resulting compound plant composition with soothing and repairing effects can bring significant soothing and anti-inflammatory effects, significantly improve the skin, and have a better effect of repairing the skin and a faster effect.

[0122] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A method for preparing a compound plant composition with soothing and repairing effects, characterized in that: The following steps are involved: Step 1), putting the composite raw material into water and soaking for 6-8 hours to obtain a mixture; Step 2), adding composite bacteria to the mixture and fermenting for 10-12 days to obtain a fermentation product; Step 3), filtering, sterilizing and concentrating the fermented product to obtain a compound plant composition with soothing and repairing effects; The composite raw material is a compound of peony root, medicinal pore fungus, and scutellaria root; The composite bacteria is a combination of Lactobacillus tucheti sausage-associated, Saccharomyces cerevisiae and Bacillus clausii. In the composite raw material, the mass ratio of peony root, medicinal pore fungus and scutellaria root is 1-3:1-3:0.3-0.7; In the composite bacteria, the mass ratio of Lactobacillus tucheti sausage-associated, Saccharomyces cerevisiae Jedinseiberlindneri, and Bacillus clausii is 1-2:1-2:1-2; The mass ratio of the composite raw material to water is 2.3-6.7:20-30; The mass ratio of the composite raw material to the composite bacteria is 2.3-6.7:0.003-0.

006.

2. The method for preparing a compound plant composition with soothing and repairing effects according to claim 1, characterized in that: In the step 3), the mass ratio of the compound plant composition with soothing and repairing effects obtained after concentration to the composite raw material added in the step 1) is 2.3-6.7:

10.

3. A method for preparing a compound plant composition with soothing and repairing effects according to claim 1 or 2, characterized in that: In the step 2), the temperature is kept at 32-34° C. and the fermentation is carried out for 10-12 days.

4. A compound plant composition with soothing and repairing effects, characterized in that: The compound plant composition with soothing and repairing effects is prepared by the preparation method of any one of claims 1-3.

5. Use of the compound plant composition with soothing and repairing effects according to claim 4 in the preparation of skin care products, characterized in that: The compound plant composition with soothing and repairing effects is added to skin care products to prepare skin care products with anti-inflammatory, soothing and repairing effects.

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