Anti-aging composition and preparation method thereof

Abstract

The present invention belongs to the technical field of medical preparations, and specifically relates to an anti-aging composition and a preparation method thereof. The anti-aging composition comprises the following raw materials in parts by weight: 5-10 parts bovine collagen tripeptide, 5-10 parts erythritol, 1-5 parts concentrated apple juice, 0.05-1 parts hydrolyzed collagen, 0.05-1 parts salmon powder, 0.05-1 parts fish maw oligopeptide, 0.05-1 parts golden bell fruit powder, 0.05-1 parts brown sugar powder, 0.05-1 parts albumin polypeptide, 0.05-1 parts sucralose, 1-5 parts anhydrous citric acid, and 55-90 parts purified water. The present invention not only supplements the collagen required by the human body, but also improves skin elasticity and scavenges free radicals.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical preparations, and in particular relates to an anti-aging composition and a preparation method thereof. Background Art

[0002] Collagen is the most abundant fibrous protein in the human body, accounting for approximately 25% of the body's total protein. It is a primary component of skin, bones, cartilage, hair, fingernails, and toenails. Numerous studies have confirmed that collagen has the dual functions of retaining skin moisture and regulating skin elasticity. However, not all collagen is effectively absorbed and utilized. The collagen that the skin truly needs is a type of collagen composed of three amino acid molecules, known as "collagen tripeptide."

[0003] The advantages of collagen tripeptide are as follows:

[0004] 1. The molecular weight of collagen is very large (100,000-300,000 Daltons), and the human body can hardly digest and absorb it directly. Collagen peptides are composed of 30-50 amino acids. After entering the body, they are randomly synthesized into proteins or collagen in various parts of the body, and the absorption rate is also low. The molecular weight of collagen tripeptide is only 250-1000 Daltons, and it can be directly absorbed and utilized by the small intestine quickly and efficiently. Under the premise of taking the same amount, the absorption rate is higher than that of ordinary collagen peptides, which can reach more than 95%.

[0005] 2. Collagen tripeptide has excellent skin permeability and can be directly transported to skin fibroblasts through the blood and can be absorbed and utilized by the skin without being decomposed. The GPH in collagen tripeptide can be deposited in the skin in a targeted manner, stimulating dermal fibroblasts to produce collagen in the skin and activating the hyaluronan synthase 2 (HAS2), which is equivalent to replenishing collagen and hyaluronic acid at the same time, repairing skin cells and replenishing moisture.

[0006] 3. Collagen tripeptide is colorless, odorless, pH stable and temperature stable. Experiments have shown that after adding collagen tripeptide, the active ingredient GPH is not decomposed by gastric acid and pepsin for up to 2 hours, showing its high stability.

[0007] Chinese patent CN 113520900 A discloses a hypoallergenic, anti-aging yak collagen composition and its application. The composition comprises the following components in percentage by mass: 1-5% yak collagen, 0.1-1.0% dipotassium glycyrrhizate, 0.5-5% white willow bark extract, 0.5-3% ellagic acid, 0.5-3% resveratrol, 0.1-0.3% acetyl hexapeptide-8, 1-3% glycerol, 0.1-1.0% vitamin E, 0.5-1.0% hyaluronic acid, 0.05-0.2% methylparaben, and 0.01-0.14% propylparaben.

[0008] Chinese patent CN 113876609 A discloses an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein and its application. In parts by weight, the anti-aging composition includes 0.1-20 parts by weight of hydroxypropyl tetrahydropyrantriol, 0.1-20 parts by weight of snake venom peptide, 0.1-20 parts by weight of recombinant human-like type III collagen, 5-30 parts by weight of butanediol and 20-70 parts by weight of deionized water.

[0009] The collagen used in the above patent cannot be effectively absorbed and utilized by the human body, and its anti-aging effect is limited. Summary of the Invention

[0010] The purpose of the present invention is to provide an anti-aging composition that can not only supplement the collagen required by the human body, but also improve skin elasticity and eliminate free radicals; the present invention also provides a preparation method of the anti-aging composition.

[0011] The anti-aging composition of the present invention is made of the following raw materials in parts by weight:

[0012] 5-10 parts of bovine collagen tripeptide, 5-10 parts of erythritol, 1-5 parts of concentrated apple juice, 0.05-1 part of hydrolyzed collagen, 0.05-1 part of salmon powder, 0.05-1 part of fish maw oligopeptide, 0.05-1 part of golden bell fruit powder, 0.05-1 part of brown sugar powder, 0.05-1 part of albumin polypeptide, 0.05-1 part of sucralose, 1-5 parts of anhydrous citric acid, 55-90 parts of purified water.

[0013] The anti-aging composition of the present invention is preferably made of the following raw materials in parts by weight:

[0014] 6 parts of bovine collagen tripeptide, 7 parts of erythritol, 3 parts of concentrated apple juice, 0.1 parts of hydrolyzed collagen, 0.08 parts of salmon powder, 0.1 parts of fish maw oligopeptides, 0.05 parts of golden bell fruit powder, 0.05 parts of brown sugar powder, 0.1 parts of albumin polypeptide, 0.02 parts of sucralose, 3 parts of anhydrous citric acid, 80.5 parts of purified water.

[0015] The method for preparing the anti-aging composition of the present invention comprises the following steps:

[0016] (1) Heating a portion of the purified water to obtain preheated purified water;

[0017] (2) Salmon powder, fish maw oligopeptide and anhydrous citric acid are mixed and dissolved in preheated purified water, an electric field is applied, brown sugar powder is added and mixed, and finally bovine collagen tripeptide, erythritol, concentrated apple juice, hydrolyzed collagen, golden bell fruit powder, albumin peptide, sucralose and the remaining purified water are added and mixed evenly to obtain a mixed solution;

[0018] (3) Filter, fill and sterilize the mixed liquid in sequence.

[0019] The heating temperature in step (1) is 50-60° C.; the mass of the purified water is 40-60% of the total mass of the purified water, preferably 50%.

[0020] The voltage of the electric field in step (2) is 50-100 V, and the time for applying the electric field is 20-30 minutes.

[0021] The filter mesh used in the filtration in step (3) has a size of 200-400 mesh, preferably 200 mesh.

[0022] The sterilization pressure in step (3) is 1-2 bar, preferably 1.6 bar; the sterilization temperature is 115-130°C, preferably 115°C; and the sterilization time is 20-45 min, preferably 40 min.

[0023] The raw materials of the anti-aging composition of the present invention also include 0.05-1 part of sea buckthorn juice, 0.05-1 part of acai juice, 0.05-1 part of pomegranate concentrated powder, 0.05-1 part of Paecilomyces elegans mycelium powder and 0.05-0.1 part of mixed fruit essence, calculated in parts by weight; preferably 0.05 part of sea buckthorn juice, 0.12 part of acai juice, 0.08 part of pomegranate concentrated powder, 0.08 part of Paecilomyces elegans mycelium powder and 0.1 part of mixed fruit essence.

[0024] The method for preparing the anti-aging composition of the present invention comprises the following steps:

[0025] (1) Heating a portion of the purified water to obtain preheated purified water;

[0026] (2) Salmon essence powder, fish maw oligopeptide and anhydrous citric acid are mixed and dissolved in preheated purified water, an electric field is applied, brown sugar powder is added and mixed, and finally bovine collagen tripeptide, erythritol, concentrated apple juice, hydrolyzed collagen, golden bell fruit powder, albumin polypeptide, sucralose, sea buckthorn juice, acai juice, pomegranate concentrate powder, Paecilomyces hymenoptera mycelium powder, mixed fruit essence and the remaining purified water are added and mixed evenly to obtain a mixed solution;

[0027] (3) Filter, fill and sterilize the mixed liquid in sequence.

[0028] The heating temperature in step (1) is 50-60° C.; the mass of the purified water is 40-60% of the total mass of the purified water, preferably 50%.

[0029] The voltage of the electric field in step (2) is 50-100 V, and the time for applying the electric field is 20-30 minutes.

[0030] The filter mesh used in the filtration in step (3) has a size of 200-400 mesh, preferably 200 mesh.

[0031] The sterilization pressure in step (3) is 1-2 bar, preferably 1.6 bar; the sterilization temperature is 115-130°C, preferably 115°C; and the sterilization time is 20-45 min, preferably 40 min.

[0032] The anti-aging composition of the present invention is a beverage product.

[0033] The beneficial effects of the present invention are as follows:

[0034] (1) The content of hydroxyproline (Hyp) in bovine collagen tripeptide is significantly higher than that in collagen tripeptide from other sources, and the triple helix structure is more stable. The product has a higher active ingredient content and a higher absorption rate by the human body.

[0035] (2) Hydrolyzed collagen has a smaller molecular weight and is easier for the human body to absorb.

[0036] (3) Concentrated apple juice can effectively neutralize the unique fishy smell of collagen tripeptide, making it more acceptable to people.

[0037] (4) The nucleoprotein in salmon powder has a direct antioxidant effect, which can synergize with fish maw oligopeptides to enhance the antioxidant activity and enhance the activity and elasticity of human skin. However, if salmon powder and fish maw oligopeptides are taken directly, they are absorbed by the human body separately, and their synergistic antioxidant effect is not obvious. In the present invention, salmon powder, fish maw oligopeptides and anhydrous citric acid are first added to water. The acidity of anhydrous citric acid is used to make the salmon powder and fish maw oligopeptides have positive charges under acidic conditions. An electric field is applied to make the salmon powder and fish maw oligopeptides aggregate together. Then, brown sugar powder is added. Brown sugar powder has viscosity after being added to water. The brown sugar powder binds the salmon powder and fish maw oligopeptides together. After bonding, the salmon powder and fish maw oligopeptides can be absorbed by the human body together, and the synergistic antioxidant effect is strong. The nucleoprotein in salmon flour contains amino and hydroxyl groups, while the amino acids in fish maw oligopeptides contain carboxyl groups. These amino and hydroxyl groups in salmon flour nucleoprotein and the carboxyl groups in fish maw oligopeptides not only attract each other but also interact with each other through intermolecular forces, forming a stable antioxidant system. Furthermore, brown sugar powder contains a large amount of hydroxyl groups, which further enhances the antioxidant capacity of both salmon flour and fish maw oligopeptides.

[0038] (5) Albumin polypeptide contains 20 essential and non-essential amino acids required by the human body, which can supplement the various amino acids required by the body. At the same time, its composition ratio is very similar to that of human serum protein (albumin), and it can replace serum protein (albumin) to maintain plasma colloidal osmotic pressure and assist in the transport of various substances in the body; it can also accelerate the removal of free radicals, play an anti-aging role and slow down the formation of skin wrinkles.

[0039] (6) Pomegranate concentrate powder is rich in bioactive substances such as punicalagin and ellagic acid. It can not only effectively inhibit and remove glycation in the body and skin, and reduce the impact of glycation on the skin, but also prevent and reduce the degradation of collagen and cell apoptosis in the skin after ultraviolet radiation, thereby playing a role in moisturizing, anti-wrinkle, anti-inflammatory, whitening and other skin beautifying effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 This is the facial skin MI value result chart.

[0041] Figure 2 This is the EI value result chart of facial skin.

[0042] Figure 3 This is a graph showing the basal cell counts containing melanin granules in mouse basal cells. DETAILED DESCRIPTION

[0043] The present invention is further described below with reference to the following examples.

[0044] Example 1

[0045] (1) Heat 40.25 parts of purified water to 60°C to obtain preheated purified water;

[0046] (2) 0.08 parts of salmon powder, 0.1 parts of fish maw oligopeptides and 3 parts of anhydrous citric acid were mixed and dissolved in preheated purified water, and an electric field was applied at a voltage of 80 V for 25 min. 0.05 parts of brown sugar powder were added and mixed. Finally, 6 parts of bovine collagen tripeptide, 7 parts of erythritol, 3 parts of concentrated apple juice, 0.1 parts of hydrolyzed collagen, 0.05 parts of golden bell fruit powder, 0.1 parts of albumin polypeptide, 0.02 parts of sucralose, 0.05 parts of sea buckthorn juice, 0.12 parts of acai juice, 0.08 parts of red pomegranate concentrate powder, 0.08 parts of Paecilomyces hymenoptera mycelium powder, 0.1 parts of mixed fruit essence and 40.25 parts of purified water were added and mixed evenly to obtain a mixed solution;

[0047] (3) The mixed liquid is filtered, filled, and sterilized in sequence to obtain the product; wherein the filter mesh used in the filtration is 200 mesh; the sterilization pressure is 1.6 bar, the sterilization temperature is 115°C, and the sterilization time is 40 minutes.

[0048] Example 2

[0049] (1) Heat 33 parts of purified water to 50°C to obtain preheated purified water;

[0050] (2) 0.1 parts of salmon powder, 1 part of fish maw oligopeptide and 2 parts of anhydrous citric acid were mixed and dissolved in preheated purified water, and an electric field was applied with a voltage of 50 V for 30 min. Then 0.1 parts of brown sugar powder was added and mixed. Finally, 5 parts of bovine collagen tripeptide, 5 parts of erythritol, 5 parts of concentrated apple juice, 1 part of hydrolyzed collagen, 0.1 parts of golden bell fruit powder, 0.05 parts of albumin polypeptide, 0.01 parts of sucralose, 0.1 parts of sea buckthorn juice, 0.05 parts of acai juice, 1 part of red pomegranate concentrated powder, 1 part of Paecilomyces hymenoptera mycelium powder, 0.05 parts of mixed fruit essence and 22 parts of purified water were added and mixed evenly to obtain a mixed solution;

[0051] (3) The mixed liquid is filtered, filled, and sterilized in sequence to obtain the product; wherein the filter mesh used in the filtration is 300 mesh; the sterilization pressure is 1 bar, the sterilization temperature is 130°C, and the sterilization time is 20 minutes.

[0052] Example 3

[0053] (1) Heat 36 parts of purified water to 55°C to obtain preheated purified water;

[0054] (2) 1 part of salmon powder, 0.2 parts of fish maw oligopeptides and 1 part of anhydrous citric acid were mixed and dissolved in preheated purified water, and an electric field was applied at a voltage of 100 V for 20 min. 0.3 parts of brown sugar powder was added and mixed. Finally, 7 parts of bovine collagen tripeptide, 8 parts of erythritol, 2 parts of concentrated apple juice, 0.3 parts of hydrolyzed collagen, 0.3 parts of golden bell fruit powder, 0.2 parts of albumin polypeptide, 0.03 parts of sucralose, 1 part of sea buckthorn juice, 1 part of acai juice, 0.05 parts of red pomegranate concentrate powder, 0.05 parts of Paecilomyces hymenoptera mycelium powder, 0.08 parts of mixed fruit essence and 54 parts of purified water were added and mixed evenly to obtain a mixed solution;

[0055] (3) The mixed liquid is filtered, filled, and sterilized in sequence to obtain the product; wherein, the filter mesh used in the filtration is 400 mesh; the sterilization pressure is 2 bar, the sterilization temperature is 120°C, and the sterilization time is 30 minutes.

[0056] Example 4

[0057] (1) Heat 40 parts of purified water to 60°C to obtain preheated purified water;

[0058] (2) 0.05 parts of salmon powder, 0.05 parts of fish maw oligopeptide and 5 parts of anhydrous citric acid were mixed and dissolved in preheated purified water, and an electric field was applied at a voltage of 70 V for 27 min. Then 1 part of brown sugar powder was added and mixed. Finally, 10 parts of bovine collagen tripeptide, 10 parts of erythritol, 1 part of concentrated apple juice, 0.05 parts of hydrolyzed collagen, 1 part of golden bell fruit powder, 1 part of albumin polypeptide, 0.05 parts of sucralose and 40 parts of purified water were added and mixed evenly to obtain a mixed solution;

[0059] (3) The mixed liquid is filtered, filled, and sterilized in sequence to obtain the product; wherein the filter mesh used in the filtration is 200 mesh; the sterilization pressure is 1.5 bar, the sterilization temperature is 115°C, and the sterilization time is 45 minutes.

[0060] Comparative Example 1

[0061] Without adding salmon powder, the other steps were the same as in Example 1.

[0062] Comparative Example 2

[0063] Without adding fish maw oligopeptide, other steps were the same as those in Example 1.

[0064] Comparative Example 3

[0065] Without adding brown sugar powder, the other steps are the same as in Example 1.

[0066] Comparative Example 4

[0067] No electric field was applied, and the other steps were the same as in Example 1.

[0068] 1. Skin test experiment:

[0069] Experimental subjects: 450 healthy women aged 18-60 years were selected.

[0070] Dosage and usage of test samples:

[0071] 450 subjects were randomly divided into 9 groups, with 50 subjects in each group, namely the control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group. The control group ate a normal diet; the Example 1 group, Example 2 group, Example 3 group, and Example 4 group consumed the products of Example 1, Example 2, Example 3, and Example 4, respectively; the Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group consumed the products of Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4, respectively, twice a day, 50 grams each time, by direct drinking. During the trial period, the original eating habits were not changed and the diet was normal.

[0072] The melanin and hemoglobin levels on the cheeks were measured using a Mexameter® MX18 skin melanin and hemoglobin test probe. The test was performed once before the experiment and again after 56 consecutive days of use. A lower MI value indicates lower melanin levels; a lower EI value indicates lower hemoglobin levels. The test principle: Based on a simple reflectance spectroscopic method, light with specific absorption spectra for melanin and hemoglobin (green light 568nm, red light 660nm, infrared light 880nm) is applied to the skin. A built-in photodetector receives the reflected light and calculates the amount of absorption and reflection from the skin, converting it into melanin and hemoglobin levels.

[0073] Note: Before the test begins, participants should cleanse their faces and rest in a constant temperature and humidity chamber to ensure their skin remains stable for 30 minutes before testing. During the test, participants should maintain their normal skincare routine, avoid switching to new skincare products, minimize outdoor exposure to strong sunlight, and refrain from travel or vacation.

[0074] Experimental results:

[0075] The results of facial skin MI value (skin melanin index) are shown in Figure 1 , the results of facial skin EI value (erythema index) are shown in Figure 2 .

[0076] Depend on Figure 1 It can be seen that taking the products of Examples 1-4 can reduce the skin MI value, and taking the products of Examples 1-4 has a more obvious effect on reducing the skin MI value than taking the products of Comparative Examples 1-4.

[0077] Depend on Figure 2 It can be seen that taking the products of Examples 1-4 can reduce the EI value of the skin, and taking the products of Examples 1-4 has a more obvious effect on reducing the EI value of the skin than taking the products of Comparative Examples 1-4.

[0078] The above results show that the anti-aging compositions prepared in Examples 1-4 can effectively improve skin condition.

[0079] 2. Mouse Experiment

[0080] Experimental subjects: 450 adult brown guinea pigs were selected.

[0081] Dosage and usage of test samples:

[0082] 450 guinea pigs were randomly divided into 9 groups, each with 50 guinea pigs, namely the control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group. The control group was fed normally; the Example 1 group, Example 2 group, Example 3 group, and Example 4 group were fed the products of Example 1, Example 2, Example 3, and Example 4, respectively; the Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group were fed the products of Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4, respectively, once a day, 20 grams each time. During the experiment, the original feeding schedule was not changed and the feeding was normal.

[0083] Before the experiment began, selected guinea pig skin sites were depilated and irradiated with UVB light at a wavelength of 310 nm for a period of time to induce skin pigmentation in the corresponding sites, after which irradiation was stopped. The animals were fed the Example and Comparative Example products once daily, 20 grams each time. On days 1, 3, 6, 9, and 12, skin biopsies were obtained from the guinea pigs, stained, and histologically observed. Densitometric analysis was performed, and the number of basal cells containing melanin granules was counted, and the average value was calculated as the percentage.

[0084] Experimental results:

[0085] Figure 2 shows the count of basal cells containing melanin granules in mouse basal cells. Figure 3 .

[0086] Depend on Figure 3 It can be seen that with the passage of time, the proportion of basal cells containing melanin granules in the basal cells of the mice in the control group continued to increase, and the proportion of basal cells containing melanin granules in the basal cells of the mice in Example 1, Example 2, Example 3 and Example 4 groups continued to decrease; the proportion of basal cells containing melanin granules in the basal cells of the mice in Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 groups also continued to decrease, but the degree of decrease was not as great as that in Example 1 group.

Claims

1. An anti-aging composition, characterized in that The invention is made of the following raw materials in parts by weight: 5-10 parts bovine collagen tripeptide, 5-10 parts erythritol, 1-5 parts concentrated apple juice, 0.05-1 part hydrolyzed collagen, 0.05-1 part salmon powder, 0.05-1 part fish maw oligopeptide, 0.05-1 part golden bell fruit powder, 0.05-1 part brown sugar powder, 0.05-1 part albumin polypeptide, 0.05-1 part sucralose, 1-5 parts anhydrous citric acid, 55-90 parts purified water; The preparation method of the anti-aging composition comprises the following steps: (1) Heating a portion of the purified water to obtain preheated purified water; (2) Salmon powder, fish maw oligopeptide and anhydrous citric acid are mixed and dissolved in preheated purified water, an electric field is applied, brown sugar powder is added and mixed, and finally bovine collagen tripeptide, erythritol, concentrated apple juice, hydrolyzed collagen, golden bell fruit powder, albumin peptide, sucralose and the remaining purified water are added and mixed evenly to obtain a mixed solution; (3) Filter, fill, and sterilize the mixed solution in sequence to obtain the product; The heating temperature in step (1) is 50-60°C; In step (2), the voltage of the electric field is 50-100 V, and the time for applying the electric field is 20-30 minutes.

2. The anti-aging composition according to claim 1, characterized in that The raw materials also include 0.05-1 part of sea buckthorn juice, 0.05-1 part of acai juice, 0.05-1 part of red pomegranate concentrated powder, 0.05-1 part of Paecilomyces hymenoptera mycelium powder and 0.05-0.1 part of mixed fruit essence, calculated by weight.

3. The anti-aging composition according to claim 1, characterized in that The mass of the purified water in step (1) is 40-60% of the total mass of the purified water.

4. The anti-aging composition according to claim 1, characterized in that The filter mesh used in the filtration in step (3) is 200-400 mesh; the sterilization pressure is 1-2 bar, the sterilization temperature is 115-130°C, and the sterilization time is 20-45 min.

5. A method for preparing the anti-aging composition according to claim 2, characterized in that The steps include: (1) Heating a portion of the purified water to obtain preheated purified water; (2) Salmon essence powder, fish maw oligopeptide and anhydrous citric acid are mixed and dissolved in preheated purified water, an electric field is applied, brown sugar powder is added and mixed, and finally bovine collagen tripeptide, erythritol, concentrated apple juice, hydrolyzed collagen, golden bell fruit powder, albumin polypeptide, sucralose, sea buckthorn juice, acai juice, pomegranate concentrate powder, Paecilomyces hymenoptera mycelium powder, mixed fruit essence and the remaining purified water are added and mixed evenly to obtain a mixed solution; (3) Filter, fill, and sterilize the mixed solution in sequence to obtain the product; The heating temperature in step (1) is 50-60°C; In step (2), the voltage of the electric field is 50-100 V, and the time for applying the electric field is 20-30 minutes.

6. The method for preparing the anti-aging composition according to claim 5, characterized in that The mass of the purified water in step (1) is 40-60% of the total mass of the purified water.

7. The method for preparing the anti-aging composition according to claim 5, characterized in that The filter mesh used in the filtration in step (3) is 200-400 mesh; the sterilization pressure is 1-2 bar, the sterilization temperature is 115-130°C, and the sterilization time is 20-45 min.

Images