Application of volatile substance in prevention and control of agricultural pest chilo suppressalis

Abstract

The invention discloses an application of a volatile substance in prevention and control of agricultural insect chilo suppressalis, and provides an application of (E)-2-hexenal (trans-2-hexenal) in prevention and control of chilo suppressalis for the first time, so that chilo suppressalis adults can be effectively repelled, the egg laying amount of chilo suppressalis female insects can be inhibited, and the prevention and control of chilo suppressalis can be realized. The natural enemy insects attracting the chilo suppressalis comprise egg parasitic wasp trichogramma japonicum and larva parasitic wasp cotesia chilo suppressalis. The (E)-2-hexenal can reduce the egg quantity of chilo suppressalis in the field, reduce the occurrence cardinal number of the chilo suppressalis, play a prevention and control role and reduce loss before pests cause harm, and meanwhile, the (E)-2-hexenal can attract natural enemy insects of the chilo suppressalis, so that natural enemies in the natural environment can be fully utilized and protected, and the economic benefit of the chilo suppressalis is improved. The improvement of the chilo suppressalis prevention and control effect and the green prevention and control are realized. (E)-2-hexenal is a component in rice volatile matters, and compared with chemical pesticides, (E)-2-hexenal is more beneficial to the ecological environment.

Description

Technical Field

[0001] The invention relates to the field of agricultural science and technology, and in particular to application of a volatile substance in the prevention and control of an agricultural pest, the striped stem borer. Background Art

[0002] Chilo suppressalis, commonly known as the stem borer, belongs to the order Lepidoptera, the superfamily Pyraloidea, the family Crambidae, and the genus Chilo Zinchin. It is listed in the "List of Class I Crop Pests and Diseases (2023)" and is a major borer of rice, threatening rice and other food production. At present, the prevention and control of the stem borer mainly relies on chemical control, but the natural survival strategy of asynchronous individual development and dormancy leads to serious overlap of generations of stem borers in the field and easy accumulation of drug resistance, which not only makes prevention and control difficult but also rapidly develops drug resistance. The effectiveness of chemical control faces great challenges, and it is urgent to explore new green and efficient control products.

[0003] The reproductive behavior of the Chilo suppressalis, which lays eggs on rice leaves, is an important step in maintaining the continuation of its population. It is also the beginning of the damage to rice. After the Chilo suppressalis larvae hatch from the eggs, they will further feed on rice and cause damage. Therefore, the development of new Chilo suppressalis control products to inhibit the Chilo suppressalis' egg-laying and other behaviors has great application value in pest control. Summary of the invention

[0004] Purpose of the invention: In view of the deficiencies in the prior art, the purpose of the present invention is to provide an application of a volatile substance in the prevention and control of the agricultural pest Chilo suppressalis. The present invention proposes for the first time the application of (E)-2-hexenal (trans-2-hexenal) in the prevention and control of Chilo suppressalis, which can effectively repel adult Chilo suppressalis, inhibit the egg laying amount of female Chilo suppressalis, and attract the natural enemy insects of Chilo suppressalis including egg parasitoids and larval parasitoids.

[0005] The present invention also provides medicines and compositions for preventing and controlling the agricultural pest Chilo suppressalis.

[0006] Technical solution: In order to achieve the above-mentioned purpose, the present invention provides an application of a volatile substance in the prevention and control of the agricultural pest Chilo suppressalis, wherein the volatile substance is (E)-2-hexenal.

[0007] Wherein, the (E)-2-hexenal also includes its composition and solvate.

[0008] The control of the striped stem borer includes repelling adult striped stem borers, inhibiting the egg-laying of female striped stem borers and attracting natural enemy insects of the striped stem borer.

[0009] Wherein, the natural enemy insects of the Chilo suppressalis are egg parasitic wasps and larval parasitic wasps.

[0010] Furthermore, the egg parasitic wasp includes Trichogramma oryzae; and the larval parasitic wasp includes Cotesia suppressalis.

[0011] The invention discloses an application of the (E)-2-hexenal in the preparation of drugs or reagents for preventing and controlling the agricultural pest Chilo suppressalis.

[0012] The (E)-2-hexenal of the present invention is used in the preparation of drugs or reagents for controlling the agricultural pest Chilo suppressalis, wherein the control includes repelling adult Chilo suppressalis, inhibiting the laying of eggs by female Chilo suppressalis and attracting natural enemy insects of Chilo suppressalis.

[0013] The pharmaceutical composition for preventing and controlling the agricultural pest Chilo suppressalis of the present invention is characterized by comprising (E)-2-hexenal as an effective ingredient and a pharmaceutically acceptable carrier or excipient thereof.

[0014] Wherein, the pharmaceutical composition is a suspension, emulsion, sustained-release agent or other technologically acceptable dosage form.

[0015] The pharmaceutical composition of the present invention is used in the preparation of drugs or reagents for controlling the agricultural pest Chilo suppressalis, wherein the control includes repelling adult Chilo suppressalis, inhibiting the egg-laying of female Chilo suppressalis and attracting natural enemy insects of Chilo suppressalis.

[0016] In the study of the interaction between the striped stem borer and rice, the present invention finds that whether the striped stem borer lays eggs on the rice or the striped stem borer larvae feed on the rice, the volatilization amount of the rice volatile (E)-2-hexenal (trans-2-hexenal) will increase. Subsequent experimental verification shows that: 1. (E)-2-hexenal has a repelling effect on the adult striped stem borer; 2. (E)-2-hexenal can inhibit the egg-laying amount of the female striped stem borer; 3. (E)-2-hexenal can attract the natural enemy insects of the striped stem borer, including the egg parasitic wasp Trichogramma oryzae and the larval parasitic wasp Corythuja striata.

[0017] Beneficial effects: Compared with the prior art, the present invention has the following advantages:

[0018] The present invention proposes for the first time the application of (E)-2-hexenal in the prevention and control of the agricultural pest Chilo suppressalis, which specifically includes that (E)-2-hexenal can reduce the number of Chilo suppressalis eggs in the field, reduce the occurrence base of Chilo suppressalis, play a prevention and control role before the pest causes harm, and reduce losses.

[0019] In the present invention, (E)-2-hexenal is used for bionic behavior regulation based on the insect olfactory mechanism, and it is difficult for pests to develop similar resistance to insecticides, which is beneficial to the application of (E)-2-hexenal in the prevention and control of striped stem borer.

[0020] The (E)-2-hexenal in the present invention can also attract natural enemy insects of the striped stem borer, can fully utilize and protect the natural enemies in the natural environment, and achieve enhanced control effect and green control of the striped stem borer.

[0021] In the present invention, (E)-2-hexenal is a component in rice volatiles and is more beneficial to the ecological environment than chemical pesticides. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The Y-tube selection experiment was used to detect the repulsive effect of (E)-2-hexenal on the adult Chilo suppressalis and the attraction of the natural enemies of the Chilo suppressalis, including: Cotesia chilonis; Trichogramma japonicum; Chilo suppressalis.

[0023] Figure 2 (E)-2-hexenal inhibits the egg-laying of female Chilo suppressalis, and the egg-laying amount is reduced compared with the control. DETAILED DESCRIPTION

[0024] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0025] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources. Experimental methods without specific conditions specified in the examples are usually carried out under conventional conditions or under conditions recommended by the manufacturer.

[0026] (E)-2-Hexenal was purchased from Yuanye Biotechnology, item number S49669-25mL, with an effective content of 98%

[0027] The wild rice variety was Zhonghua 11, which was provided by Jiangsu Academy of Agricultural Sciences.

[0028] The mutant rice is Zhonghua 11 defective in producing (E)-2-hexenal, and the mutant rice seeds are Zhonghua 11 rice OsHPL3 mutant seeds (LOC_Os02g02000) lacking HPL3 function, which were purchased from Biogle (http: / / biogle.cn / ) with the germplasm number BG110627F11.

[0029] Example 1

[0030] (E)-2-hexenal has a repelling effect on adult Chilo suppressalis and attracts natural enemies of Chilo suppressalis

[0031] The detection device for the selection behavior of adult Chilo suppressalis and parasitic wasps on (E)-2-hexenal is a transparent glass Y-shaped tube. An atmospheric sampler is used as the airflow power system. The air blown out by the atmospheric sampler is filtered through activated carbon and molecular sieves, and blown into the two arms of the "Y"-shaped tube through the flavor source bottle. The flavor source bottle contains filter paper strips with 5ng / ul10ul of (E)-2-hexenal (diluted with n-hexane) and control reagent n-hexane. The gas flow rate is set to 0.2L / min, and the device is connected by a silicone tube. The single head of the test insect is placed in the main arm of the "Y"-shaped tube, and the selection of the test insect within 10 minutes is recorded. When the test insect enters a branch arm and moves a distance exceeding 1 / 3 of the length of the branch arm and stays for 30s or more, it is recorded as choosing the arm; if the test insect does not make a choice within 10 minutes, it is not counted. After a total of 10 insects have made a choice, the "Y"-shaped tube was cleaned with anhydrous ethanol, and the positions of the branch arms of the experimental group and the control group were swapped after drying to eliminate the influence of the position effect on the behavioral choice. The behavioral choice of female adults of the Chilo suppressalis was carried out in the dark period, and red light was used as the light source. The behavioral choice experiment of the parasitic wasp was completed during the day.

[0032] like Figure 1 As shown, the experimental results showed that (E)-2-hexenal had a repelling effect on the adult of Chilo suppressalis, and had an attractive effect on the egg parasitoid Trichogrammatus oryzae and the larval parasitoid Trichogrammatus oryzae.

[0033] Example 2

[0034] (E)-2-Hexenal inhibits oviposition of female Chilo suppressalis

[0035] The production of (E)-2-hexenal in wild-type rice (Zhonghua 11) increased after being attacked by insect pests, but the production of (E)-2-hexenal in mutant rice defective in producing (E)-2-hexenal did not change significantly when attacked by the same pests (Wang Baohui. (2013). Functional analysis of rice insect resistance-related genes OsICS and OsHPL3, Zhejiang University]).

[0036] 1. Detection of Chilo suppressalis re-laying eggs after pretreatment of rice with Chilo suppressalis adult eggs

[0037] Wild-type rice (Zhonghua 11) and mutant rice (Zhonghua 11 with defects in producing (E)-2-hexenal) were pretreated for egg laying in a greenhouse. 21-day-old rice (whole rice plants planted in plastic cups) were placed in insect cages (40cm×40cm×40cm), and then 30 pairs of male and female adults of Chilo suppressalis were released to lay eggs. After 24 hours, the rice was removed and rice with egg masses on the leaves was selected for re-egg laying detection. The detection method was to place 2 cups of pretreated wild-type rice (diagonally placed) and 2 cups of mutant rice (diagonally placed) in a new insect cage, a total of 4 cups of rice, and then 30 pairs of male and female adults of Chilo suppressalis were placed to lay eggs. After 72 hours, the rice was removed and the number of all eggs on the rice leaves was counted.

[0038] 2. Detection of oviposition of Chilo suppressalis after larvae feed on pretreated rice

[0039] Wild-type rice and mutant rice were pretreated for egg laying in a greenhouse. 21-day-old rice (whole rice plants planted in plastic cups) were placed in insect cages (40cm×40cm×40cm), and each cup of rice was inoculated with 3rd-instar larvae of the Chilo suppressalis that had been starved for 12 hours for feeding. After 24 hours, the larvae were removed and the fed rice was used for egg laying detection. Two cups of wild-type and mutant rice were placed in a new insect cage, respectively, at the four corners of the cage, with the same rice placed diagonally. Then 30 pairs of male and female Chilo suppressalis adults were placed in the cage for egg laying. After 72 hours, the Chilo suppressalis eggs on the rice leaves were collected and counted for analysis.

[0040] like Figure 2 As shown, the results show that the egg-laying amount of the mutant rice (blue) defective in producing (E)-2-hexenal of the Chilo suppressalis was significantly higher than that of the normal wild-type rice (red), whether after egg-laying damage induction or larval feeding induction, indicating that (E)-2-hexenal can inhibit the egg-laying of female Chilo suppressalis. In summary, (E)-2-hexenal can be used to prepare drugs or reagents for the control of agricultural pest Chilo suppressalis.

Claims

1. An application of a volatile substance in the prevention and control of the agricultural pest Chilo suppressalis, characterized in that: The volatile substance is (E)-2-hexenal.

2. The use according to claim 1, characterized in that: The (E)-2-hexenal also includes its composition and solvate.

3. The use according to claim 1, characterized in that: The control of the striped stem borer includes repelling the adult striped stem borer, inhibiting the egg-laying of the female striped stem borer and attracting the natural enemy insects of the striped stem borer.

4. The use according to claim 3, characterized in that: The natural enemy insects of the Chilo suppressalis are preferably egg parasitic wasps and larval parasitic wasps.

5. The use according to claim 4, characterized in that: The egg parasitic wasp includes Trichogramma oryzae; and the larval parasitic wasp includes Codonopsis pilosa.

6. Application of (E)-2-hexenal in the preparation of drugs or reagents for controlling the agricultural pest Chilo suppressalis.

7. Use of (E)-2-hexenal in the preparation of drugs or reagents for controlling the agricultural pest Chilo suppressalis, wherein the control comprises repelling adult Chilo suppressalis, inhibiting the laying of eggs by female Chilo suppressalis and attracting natural enemy insects of Chilo suppressalis.

8. A pharmaceutical composition for preventing and controlling the agricultural pest Chilo suppressalis, characterized in that: The invention comprises (E)-2-hexenal as an effective ingredient and a pharmaceutically acceptable carrier or auxiliary material.

9. The pharmaceutical composition according to claim 8, characterized in that The pharmaceutical composition is a suspension, an emulsion or a sustained-release agent.

10. Use of the pharmaceutical composition according to claim 8 in preparing drugs or agents for controlling the agricultural pest Chilo suppressalis, wherein the control comprises repelling adult Chilo suppressalis, inhibiting the oviposition of female Chilo suppressalis and attracting natural enemy insects of Chilo suppressalis.

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