Composition containing golden camellia extract for anti-pollution and acne-sensitive skin repair and its application

Abstract

The present invention discloses a composition for repairing acne-sensitive skin with an anti-pollution property containing a camellia chrysantha extract and its application, relating to the technical field of cosmetics. The present invention provides a composition for repairing acne-sensitive skin with an anti-pollution property containing a camellia chrysantha extract, comprising the following components in parts by weight: 1-5 parts of camellia chrysantha extract, 1-5 parts of seaweed oligosaccharides, 0.01-1 parts of o-cymene-5-ol, and 0.01-1 parts of tocopheryl retinoate. The composition for repairing acne-sensitive skin with an anti-pollution property containing a camellia chrysantha extract of the present invention, by selecting the four components of camellia chrysantha extract, seaweed oligosaccharides, o-cymene-5-ol, and tocopheryl retinoate in appropriate parts by weight, has better anti-pollution and antibacterial effects, and has excellent acne-removing, repairing, and soothing effects.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to a composition containing a golden camellia flower extract for resisting pollution and repairing acne-sensitive skin and application thereof. Background Art

[0002] Acne-prone skin is characterized by easily clogged pores and excessive oil secretion, often accompanied by inflammatory acne and papules, and prone to redness, swelling, and uneven skin. Sensitive skin, on the other hand, is highly sensitive to external stimuli, often experiencing discomfort such as stinging, tightness, and redness, and reacts strongly to the ingredients in skincare products. The combination of these two skin types, resulting in acne-sensitive skin, poses an even more severe challenge. This skin type is both prone to acne and suffers from severe inflammation, leading to recurring flare-ups and a lack of recovery. Furthermore, the compounded sensitivity prevents the use of many common skincare products, resulting in a lack of effective treatment options. This increases psychological burden and self-esteem, impacting quality of life.

[0003] Environmental pollution, particularly air pollution, including cooking fumes (COFs), smog (PM2.5), cigarette smoke (CS), and the germs that grow from unwashed indoor air conditioners, all pose significant risks to acne-prone skin. Cooking fumes can clog pores and release chemicals that can cause skin inflammation and contribute to breakouts. PM2.5 particles in smog not only easily penetrate the skin, causing oxidative stress but also degrade the skin barrier, increasing the risk of allergic reactions. Toxins in cigarette smoke can also irritate the skin, encouraging the growth of Propionibacterium acnes and worsening acne. Unwashed air conditioners breed mold and bacteria, further irritating sensitive skin and worsening allergies and breakouts. This negative impact is particularly unavoidable for office workers who run their air conditioners for cooling in the summer and heating in the winter. These combined environmental factors exacerbate the problems of acne-prone skin, and this double pressure makes it even more difficult for skin to recover.

[0004] Currently, most products focus solely on anti-inflammatory and repairing the skin, neglecting the importance of "protection." External pollution, especially air pollution, is present in every aspect of life, making it virtually impossible to avoid. Its harmful effects on acne are often overlooked. Effective acne treatment relies on simultaneously treating acne and protecting against external pollution, not only preventing breakouts but also preventing existing ones from worsening, ultimately restoring skin health. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide an anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract, which has anti-pollution, antibacterial, and excellent acne-removing, repairing and soothing effects and its application.

[0006] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: a composition for anti-pollution and acne-sensitive skin repair containing Camellia chrysantha extract, comprising the following components in parts by weight: 1-5 parts of Camellia chrysantha extract, 1-5 parts of seaweed oligosaccharides, 0.01-1 parts of o-cymene-5-ol, and 0.01-1 parts of tocopheryl retinoate.

[0007] Camellia chrysantha extract contains a large number of flavonoids and polyphenols, such as camelliaside A and catechins. These compounds possess significant antioxidant properties, neutralizing free radicals and thus reducing oxidative damage caused by harmful airborne substances such as cooking fumes (COFs), haze (PM2.5), and cigarette smoke (CS). Camellia chrysantha extract also possesses anti-inflammatory properties, alleviating inflammatory responses to the skin caused by environmental pollution. It also possesses antibacterial properties, helping to inhibit the growth of pathogens in air-conditioned indoor environments, further reducing the risk of acne. Seaweed oligosaccharides are oligosaccharides obtained by degrading natural seaweed polysaccharides. They have the advantages of low molecular weight and good water solubility. They possess significant antioxidant and anti-inflammatory properties. Furthermore, seaweed oligosaccharides contain numerous carboxyl and hydroxyl groups, which can form hydrogen bonds or electrostatic interactions with airborne pollutants, promoting their adsorption to the skin surface. This property enables seaweed oligosaccharides to capture and remove pollutants from the skin's surface, thereby reducing their damage. Due to its hydrophilicity and adhesion, seaweed oligosaccharides can also form a protective film on the skin surface, blocking direct contact with external pollutants, preventing pollutants in the air from entering the skin and clogging pores, and playing a protective and repairing role.

[0008] o-Cymene-5-ol is a water-soluble cymene with a wide range of bactericidal properties. It has a good killing effect on pathogens produced by unclean air-conditioning air. It can also improve skin hydration, enhance the barrier function of the stratum corneum, and further resist the invasion of harmful external substances. It also has a certain effect of softening cuticles and increasing the shedding of old and dead epithelial cells, which can effectively remove acne, and can promote the penetration of active ingredients. It has a certain whitening effect and can improve acne scars. Tocopheryl retinoate is an innovative ingredient that combines vitamin E and vitamin A. It not only has the antioxidant effect of vitamin E, but also has the beneficial effects of vitamin A on improving skin texture and inhibiting keratosis pilaris. It also has both oil control and whitening effects. This ingredient also has significant anti-inflammatory properties, which can effectively relieve skin discomfort caused by environmental irritation or inflammation, and promote skin repair and regeneration.

[0009] The anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract of the present invention is prepared by selecting appropriate weight portions of four components, namely, Camellia chrysantha extract, seaweed oligosaccharides, o-cymene-5-ol, and tocopheryl retinoate, to work synergistically. First, a protective film can be formed to improve skin hydration, enhance the barrier function of the stratum corneum, and block direct contact with external pollutants. Second, after a small amount of external pollutants invade, the various components work synergistically to reduce oxidative damage caused by harmful substances in the air, such as cooking fumes (COFs), haze (PM2.5), and cigarette smoke (CS). Third, the various components of the present invention work synergistically to have a better anti-inflammatory effect, can effectively relieve skin discomfort caused by environmental irritation or inflammation, and promote skin repair and regeneration.

[0010] Preferably, the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract comprises the following components in parts by weight: 2-4 parts of Camellia chrysantha extract, 2-4 parts of seaweed oligosaccharides, 0.05-0.5 parts of o-cymene-5-ol, and 0.05-0.5 parts of tocopheryl retinoate.

[0011] During the actual research and development process, the inventors found that the weight proportions of the four components, Camellia chrysantha extract, seaweed oligosaccharides, o-cymene-5-ol, and tocopheryl retinoate, will affect the performance of the final product. When the four components, Camellia chrysantha extract, seaweed oligosaccharides, o-cymene-5-ol, and tocopheryl retinoate, are within the above range, the anti-pollution, antibacterial, acne-removing, and repairing and soothing effects are better.

[0012] Preferably, the weight ratio of the Camellia chrysantha extract to seaweed oligosaccharides is (1-2):1; further preferably, the weight ratio of the Camellia chrysantha extract to seaweed oligosaccharides is 1:1.

[0013] Preferably, the weight ratio of o-cymene-5-ol and tocopheryl retinoate is (1-2):1; further preferably, the weight ratio of o-cymene-5-ol and tocopheryl retinoate is 1:1.

[0014] During the actual research and development process, the inventors found that the weight ratio of Camellia chrysantha extract and seaweed oligosaccharides, as well as the weight ratio of o-cymene-5-ol and tocopheryl retinoate in the anti-pollution and acne-repairing composition containing Camellia chrysantha extract will further affect the performance of the final product. When the weight ratio is within the above range, the anti-pollution, antibacterial, acne-removing, and repairing and soothing effects are better.

[0015] Preferably, the seaweed oligosaccharide comprises at least one of seaweed oligosaccharide-I, seaweed oligosaccharide-II, seaweed oligosaccharide-III, and seaweed oligosaccharide-IV. Further preferably, the seaweed oligosaccharide is seaweed oligosaccharide-III.

[0016] The seaweed oligosaccharide of the present invention is polymerized from 4 to 9 monosaccharide units and has a molecular weight of 500Da-1000Da. During the actual research and development process, the inventors found that when the seaweed oligosaccharide is polymerized from 4 to 9 monosaccharide units and has a molecular weight of 500Da-1000Da, the short-chain oligosaccharides polymerized from 4 to 9 monosaccharide units are more easily absorbed by the skin and can quickly penetrate into the surface of the skin. They can absorb moisture and form a protective film on the skin, improving skin feel and moisture content. Too many monosaccharide units are more suitable for forming a high-viscosity colloidal substance, which can form a stronger barrier on the skin surface to prevent moisture loss and is more suitable for dry skin care. However, for acne-prone skin, its skin feel can easily become a skin burden, and the effect it plays is not what acne-sensitive skin needs most, so the acne-removing and repairing effect will be much worse.

[0017] Preferably, the preparation method of the Camellia chrysantha extract comprises the following steps: mixing Camellia chrysantha powder with ethanol aqueous solution, then extracting at 55-65°C for 25-35 minutes, then ultrasonically extracting at 55-65°C for 55-65 minutes, and then concentrating the extract and drying to obtain the Camellia chrysantha extract.

[0018] Preferably, the volume percentage of ethanol in the ethanol aqueous solution is 60-80%; and / or the weight ratio of the golden camellia powder to the ethanol aqueous solution is 1:(5-20); and / or the ultrasonic power is 280-300W; and / or the temperature of the concentrated extract is 45-55°C.

[0019] Further preferably, the weight ratio of the Camellia chrysantha powder to the ethanol aqueous solution is 1:(5-10).

[0020] Preferably, the preparation method of the golden camellia powder is: collecting the golden camellia flowers, washing them, placing them in a constant temperature oven at 60-70°C and drying them to constant weight, then grinding and crushing them and passing them through a 100-150 mesh sieve, collecting the sieve residue to obtain the golden camellia powder.

[0021] In addition, the present invention provides the use of the anti-pollution and acne-sensitive skin repairing composition containing the Camellia chrysantha extract in the preparation of skin products.

[0022] Furthermore, the present invention provides a skin product, which includes the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract; the skin product is one of a lotion, emulsion, cream, facial mask, essence, and spray.

[0023] In addition, the present invention provides a skin care product comprising the following components in percentage by mass: 1-5% of an anti-pollution and acne-sensitive skin repairing composition containing a Camellia chrysantha extract, 1-40% of a cosmetic base, and the remainder being water.

[0024] The invention provides a skin product, which is an essence and comprises the following components in percentage by mass: 1-5% of an anti-pollution and acne-sensitive skin repairing composition containing a golden camellia flower extract, 0.05-0.5% of a thickener, 0.5-1% of a moisturizer, 0.5-5% of an emulsifier, 0.5-3% of a preservative, 0.01-0.3% of a pH regulator, and the balance being water.

[0025] Optionally, the essence comprises at least one of the following (a)-(e):

[0026] (a) the thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol;

[0027] (b) the moisturizing agent comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-propylene glycol, 1,2-butylene glycol, glycerin, budding sclerotin polysaccharide, ceramide, and D-panthenol;

[0028] (c) the pH adjusting agent comprises at least one of arginine, tromethamine, and EDTA-disodium;

[0029] (d) the emulsifier comprises at least one of ethylhexylglycerin, dimethicone, steareth-2, steareth-2, caprylic capric triglyceride, polymethylsilsesquioxane, cetearyl glucoside, cetearyl olivate, and C14-22 alcohol / C12-20 alkyl glucoside;

[0030] (e) The preservative includes at least one of 1,2-hexanediol, 1,2-pentanediol, and p-hydroxyacetophenone.

[0031] Furthermore, the present invention provides a method for preparing the essence, comprising the following steps:

[0032] (1) Mix the humectant and thickener with water, heat to 85±2°C, and homogenize at 1200 rpm for 4 minutes. After homogenization, keep the mixture warm for later use to obtain prefabricated component A;

[0033] (2) After heating the emulsifier to 85±2°C, homogenize at 1200 rpm for 4 minutes. After homogenization, keep the mixture warm for later use to obtain prefabricated component B;

[0034] (3) Mix the preservatives and heat to 60±2°C to melt to obtain prefabricated component C;

[0035] (4) Components A and B were mixed at 80°C and homogenized at 1200 rpm for 6 min until a uniform liquid was formed. The mixture was cooled to 40°C and the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract was added and continued to be stirred for 8 min. Finally, a pH regulator was added to adjust the pH to 6.0. The stirring was stopped and the material was discharged to obtain the essence.

[0036] Compared with the prior art, the beneficial effects of the present invention are as follows: the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract of the present invention, by selecting appropriate weight portions of four components, namely Camellia chrysantha extract, seaweed oligosaccharides, o-cymene-5-ol, and tocopheryl retinoate, can, first, form a protective film, improve skin hydration, enhance the barrier function of the stratum corneum, and block direct contact with external pollutants; second, after a small amount of external pollutants invade, the various components can work together to reduce oxidative damage caused by harmful substances in the air such as cooking fumes (COFs), haze (PM2.5) and cigarette smoke (CS); third, the various components of the present invention work together to have a better anti-inflammatory effect, can effectively relieve skin discomfort caused by environmental irritation or inflammation, and promote skin repair and regeneration. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 Graphs showing the inhibitory effects of the compositions prepared in Examples and Comparative Examples on bacteria and fungi in unclean air conditioners; wherein (a) shows the inhibitory effects on bacteria in unclean air conditioners, and (b) shows the inhibitory effects on fungi in unclean air conditioners;

[0038] Figure 2 These are the pictures showing the effects of the essence prepared in the blank application example, implementation application example 1 and comparison application example 1 on removing acne, improving red blood streaks and repairing skin. DETAILED DESCRIPTION

[0039] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below with reference to the accompanying drawings and specific examples. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0040] The raw materials used in the examples and comparative examples of the present invention are described below, but are not limited to the following raw materials:

[0041] Seaweed oligosaccharide-I, from Haisheng Health Biotechnology Co., Ltd.;

[0042] Seaweed oligosaccharide-II, from Haisheng Health Biotechnology Co., Ltd.;

[0043] Seaweed oligosaccharide-III, from Haisheng Health Biotechnology Co., Ltd.;

[0044] Seaweed oligosaccharide-IV, from Haisheng Health Biotechnology Co., Ltd.;

[0045] o-Cymen-5-ol was obtained from Guangzhou Tongjun Pharmaceutical Technology Co., Ltd.;

[0046] Tocopheryl retinoate was obtained from Shanghai Keqin Technology Co., Ltd.

[0047] Camellia chrysantha extract 1: homemade, preparation method: collect Camellia chrysantha flowers, wash and dry in a 65°C constant temperature oven to constant weight, grind and crush, pass through a 150-mesh sieve, collect the sieve residue to obtain Camellia chrysantha powder; add 8 times the volume of 70% ethanol aqueous solution to the Camellia chrysantha powder, and extract at 60°C for 30 minutes; then extract at an ultrasonic power of 280W and a temperature of 60°C for 1 hour; after the extraction, filter and collect the extract, and concentrate the extract by rotary evaporation at 50°C. After the concentration, dry and freeze in an ultra-low temperature freeze dryer to obtain Camellia chrysantha extract 1;

[0048] Camellia chrysantha extract 2: homemade, preparation method: collect Camellia chrysantha flowers, wash and dry in a 65°C constant temperature oven to constant weight, grind and crush, pass through a 100-mesh sieve, collect the sieve residue to obtain Camellia chrysantha powder; add 15 times the volume of 65% ethanol aqueous solution to the Camellia chrysantha powder, and extract at 65°C for 25 minutes; then extract at an ultrasonic power of 290W and a temperature of 55°C for 1 hour; after the extraction, filter and collect the extract, and concentrate the extract by rotary evaporation at 45°C. After the concentration, dry and freeze in an ultra-low temperature freeze dryer to obtain Camellia chrysantha extract 2;

[0049] Camellia chrysantha extract 3: homemade, preparation method: collect Camellia chrysantha flowers, wash and dry in a 65°C constant temperature oven to constant weight, grind and crush, pass through a 100-mesh sieve, collect the sieve residue to obtain Camellia chrysantha powder; add 20 times the volume of 80% ethanol aqueous solution to the Camellia chrysantha powder, and extract at 60°C for 30 minutes; then extract at an ultrasonic power of 300W and a temperature of 65°C for 1 hour; after the extraction is completed, filter and collect the extract, and concentrate the extract by rotary evaporation at 55°C. After concentration, dry and freeze in an ultra-low temperature freeze dryer to obtain Camellia chrysantha extract 3;

[0050] Camellia extract, manufactured by Jiangsu Jufeng Chemical Technology Co., Ltd.

[0051] Xinqiong hexasaccharide, manufactured by Blue Brain Technology (Xiamen) Co., Ltd., is composed of 6 monosaccharide units;

[0052] Totarol, manufacturer: Tesco Chemical (Hubei) Co., Ltd.

[0053] Bakuchiol, manufactured by Shaanxi Fuheng Biotechnology Co., Ltd.

[0054] Examples and Comparative Examples

[0055] The present invention provides an anti-pollution and acne-sensitive skin repairing composition containing a Camellia chrysantha extract. The components and weight portions of the anti-pollution and acne-sensitive skin repairing composition containing a Camellia chrysantha extract are shown in Tables 1 and 2.

[0056] Table 1

[0057]

[0058]

[0059] Table 2

[0060]

[0061] Table 3

[0062]

[0063]

[0064] The present invention provides a method for preparing the essence, comprising the following steps:

[0065] (1) Mix the humectant and thickener with water, heat to 85±2°C, and homogenize at 1200 rpm for 4 minutes. After homogenization, keep the mixture warm for later use to obtain prefabricated component A.

[0066] (2) After heating the emulsifier to 85±2°C, homogenize at 1200 rpm for 4 minutes. After homogenization, keep the mixture warm for later use to obtain prefabricated component B;

[0067] (3) Mix the preservatives and heat to 60±2°C to melt to obtain prefabricated component C;

[0068] (4) Components A and B were mixed at 80°C and homogenized at 1200 rpm for 6 min until a uniform liquid was formed. The mixture was cooled to 40°C and the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract was added and continued to be stirred for 8 min. Finally, a pH regulator was added to adjust the pH to 6.0. The stirring was stopped and the material was discharged to obtain the essence.

[0069] The preparation methods of the remaining application examples, comparative application examples, and blank application examples are the same as those of Application Example 1, except that no relevant components are added if they are not present.

[0070] Performance Test-1: Inhibitory effect of the composition on external cooking fumes (COFs), haze (PM2.5) and cigarette smoke (CS).

[0071] Air pollutants are one of the main causes of recurring acne and skin sensitivity in people with acne-sensitive skin. These pollutants can induce keratinocyte death, worsening skin conditions. Therefore, by measuring the effects of the Examples and Comparative Examples on keratinocyte viability in the presence of pollutants, if cell viability is increased compared to the group containing only the pollutants, it demonstrates that the composition has an anti-pollution effect. The stronger the cell viability, the better the anti-pollution effect.

[0072] Experimental Methods: Haze particulate matter (PM2.5) was collected over 24 hours using a Th-150c III mid-flow air sampler with a quartz filter. Smoking volunteers were recruited to generate cigarette smoke in a closed room and collected using an air sampler with a quartz filter (CS). Cooking fume (COF) was also collected using an air sampler with a quartz filter during cooking. Samples were immediately stored at -20°C.

[0073] The air sampler quartz filter containing these air pollutants was then cut into pieces and extracted using n-hexane / dichloromethane (1 / 1, v / v) by microwave ultrasound for 24 hours. The organic extract was concentrated to approximately 1 mL using a rotary evaporator, and the pollutants were diluted with dimethyl sulfoxide (DMSO) and stored at -20°C.

[0074] Human keratinocytes (HaCaT) were revived and cultured for 24 hours using MEM containing 10% FBS as a culture medium. The anti-pollution and acne-sensitive skin repair compositions containing Camellia chrysantha extracts of the examples and comparative examples of the present invention were diluted to 2% (v / v) using DMSO. The cells in the sample group were treated with stimulant solutions of PM2.5 (100 μg / mL), CS (100 μg / mL), and COF (10 mg / mL) and solutions of each example and comparative example for 24 hours, while the control group was treated with only stimulant solutions of PM2.5, CS, and COF for 24 hours. The different compositions were configured into sample solutions and co-cultured with HaCaT cells, after which the cells were harvested. The cell viability of HaCaT cells in the sample and control groups was detected using an MTT kit.

[0075] Cell activity improvement rate (%) = (cell activity of sample group - cell activity of control group) / cell activity of control group × 100%; the obtained results are shown in Table 4.

[0076] Table 4

[0077]

[0078]

[0079] As can be seen from the above table, the anti-pollution and acne-sensitive skin repairing composition containing the Camellia chrysantha extract prepared in the embodiment of the present invention has an improvement rate of cellular activity of PM2.5, CS, and COF of ≥20%.

[0080] From the comparison of Examples 1-5, it can be seen that the weight ratio of Camellia chrysantha extract and seaweed oligosaccharides, as well as the weight ratio of o-cymene-5-ol and tocopheryl retinoate in the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract will further affect the performance of the final product. When the weight ratio of the Camellia chrysantha extract and seaweed oligosaccharides is (1-2):1; and / or the weight ratio of o-cymene-5-ol and tocopheryl retinoate is (1-2):1, the cell activity improvement rate of PM2.5, CS, and COF is better. When the weight ratio of the Camellia chrysantha extract and seaweed oligosaccharides is 1:1; and / or the weight ratio of o-cymene-5-ol and tocopheryl retinoate is 1:1, the cell activity improvement rate of PM2.5, CS, and COF reaches the best.

[0081] From the comparison of Example 1 and Examples 6-7, it can be seen that the extraction method of Camellia chrysantha will affect the cell activity improvement rate of PM2.5, CS, and COF. When the weight ratio of Camellia chrysantha powder to ethanol aqueous solution is 1: (5-10), the cell activity improvement rate of PM2.5, CS, and COF is better.

[0082] From the comparison of Example 1 and Examples 8-10, it can be seen that the type of seaweed oligosaccharide will affect the improvement rate of cell activity for PM2.5, CS, and COF. When the seaweed oligosaccharide is seaweed oligosaccharide-III, the improvement rate of cell activity for PM2.5, CS, and COF is better.

[0083] From the comparison of Example 1 and Comparative Examples 1-8, it can be seen that the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract of the present invention comprises four components: Camellia chrysantha extract, seaweed oligosaccharides, o-cymene-5-ol, and tocopheryl retinoate, all of which work synergistically and are indispensable.

[0084] Performance Test-2 Inhibitory Effect of the Composition on Bacteria and Fungi in Dirty Air Conditioners.

[0085] Common bacteria found in unclean air conditioners include Staphylococcus, Escherichia coli, and molds such as Penicillium and Black Mold. These microorganisms thrive in humid environments, releasing allergens and toxins that can exacerbate skin inflammation and lead to acne. For acne-prone skin, the presence of bacteria and fungi can increase sebum secretion, leading to clogged pores and infection, which can cause acne and skin discomfort, worsening skin condition and increasing the risk of recurrence. The effectiveness of a composition in killing harmful bacteria and fungi in air conditioners can be used to assess its antibacterial and anti-pollution capabilities; the stronger the killing effect, the stronger the anti-pollution ability.

[0086] Experimental method: configure meat extract peptone liquid medium (LB medium), configure potato glucose broth (PDB medium), wipe the fan blade of the dirty air conditioner with a disposable cotton swab, and place it in LB medium and PDB medium respectively. LB medium is used to culture the bacteria contained in the air conditioner for 48 hours, and PDB medium is used to culture the fungi contained in the air conditioner for 48 hours to form a bacterial liquid. The composition of the embodiment and the comparative example is configured with DMSO to become a 1% sample liquid for standby use. 180 μL of bacterial liquid is added to a 96-well plate, and then 40 μL of each composition is added. The control group is set to not add the composition. After culturing for 24 hours, the OD value of each well is measured with a microplate reader. The smaller the OD value, the clearer the liquid, the worse the activity of the bacteria, and the better the antibacterial effect of the composition.

[0087] Inhibition rate (%) = (OD value of control group - OD value of sample group) / OD value of control group × 100%; the results are shown in Table 5.

[0088] Table 5

[0089]

[0090]

[0091] As can be seen from the above table, the anti-pollution and acne-sensitive skin repairing composition containing the Camellia chrysantha extract prepared in the embodiment of the present invention has an inhibition rate of ≥60% on bacteria and fungi in unclean air conditioners. Figure 1 The figures show the inhibitory effects of the compositions prepared in the examples and comparative examples on bacteria and fungi in unclean air conditioners; wherein, (a) shows the inhibitory effects on bacteria in unclean air conditioners, and (b) shows the inhibitory effects on fungi in unclean air conditioners, respectively presenting the inhibitory effects of the anti-pollution and acne-sensitive skin repairing compositions containing Camellia chrysantha extract prepared in Examples 1-10 and Comparative Examples 1-8 on bacteria and fungi.

[0092] From the comparison of Examples 1-5, it can be seen that the weight ratio of Camellia chrysantha extract and seaweed oligosaccharides, as well as the weight ratio of o-cymene-5-ol and tocopheryl retinoate in the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract will further affect the performance of the final product. When the weight ratio of the Camellia chrysantha extract and seaweed oligosaccharides is (1-2):1; and / or the weight ratio of o-cymene-5-ol and tocopheryl retinoate is (1-2):1, the inhibition rate of bacteria and fungi in unclean air conditioners is better. When the weight ratio of the Camellia chrysantha extract and seaweed oligosaccharides is 1:1; and / or the weight ratio of o-cymene-5-ol and tocopheryl retinoate is 1:1, the inhibition rate of bacteria and fungi in unclean air conditioners reaches the best.

[0093] From the comparison of Example 1 and Examples 6-7, it can be seen that the extraction method of Camellia chrysantha will affect the inhibition rate of bacteria and fungi in unclean air conditioners. When the weight ratio of Camellia chrysantha powder to ethanol aqueous solution is 1: (5-10), the inhibition rate of bacteria and fungi in unclean air conditioners is higher.

[0094] From the comparison between Example 1 and Examples 8-10, it can be seen that the type of seaweed oligosaccharide has little effect on the inhibition rate of bacteria and fungi in unclean air conditioners.

[0095] From the comparison of Example 1 and Comparative Examples 1-8, it can be seen that the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract of the present invention comprises four components: Camellia chrysantha extract, seaweed oligosaccharides, o-cymene-5-ol, and tocopheryl retinoate, all of which work synergistically and are indispensable.

[0096] Performance Test-3 Composition has acne-removing, repairing and soothing effects on acne-sensitive skin.

[0097] This effect example tests the effects of the application example, comparative application example and blank application example on human skin TEWL, acne count and red blood streak improvement, and respectively detects the effects of the essence prepared by the application example of the present invention on barrier repair, acne removal and sensitivity relief of acne-sensitive skin.

[0098] The specific test method is as follows: According to the "Technical Specifications for Safety of Cosmetics" (2015), volunteers with sensitive skin aged 18-50 years (lactic acid stinging score > 3) were selected and randomly divided into 20 groups, with 5 people in each group. The volunteers had obvious acne, blackheads, and papules. Under normal circumstances, the volunteers continued to use the product on their entire face for 14 days; every morning and evening after cleansing and moisturizing, the volunteers applied an appropriate amount of essence to the face and massaged it for absorption. The acne area can be overlapped and the entire face is used once in the morning and evening, and a follow-up visit was conducted after 14 days.

[0099] On the day of their visit, volunteers washed their faces with clean water in the test area without applying any products. They then sat quietly in an air-conditioned room at a temperature of 21±1°C and a humidity of 50±10% for 20 minutes. Improvement in facial acne indicators was quantified and photographed using instruments, including acne counting (counting the total number of comedones and papules, assessed using VISIA and a physician), Tewameter™ Hex testing for transepidermal water loss (TEWL), and MX 18 testing for redness.

[0100] Improvement rate before and after use = |mean value before use - mean value after use| / mean value before use × 100%; the calculation results are shown in Table 6. The improvement rate presented in Table 6 is the improvement rate at the 14th day follow-up.

[0101] Table 6

[0102]

[0103]

[0104] As can be seen from the above table, the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract prepared in the embodiment of the present invention has a good effect of improving acne, repairing and soothing, and improving red blood streaks when applied to essence. Figure 2 These are the pictures showing the effects of the essence prepared in the blank application example, implementation application example 1 and comparison application example 1 on removing acne, improving red blood streaks and repairing skin.

[0105] From the comparison of Examples 1-5, it can be seen that the weight ratio of Camellia chrysantha extract and seaweed oligosaccharides, as well as the weight ratio of o-cymene-5-ol and tocopheryl retinoate in the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract will further affect the performance of the final product. When the weight ratio of the Camellia chrysantha extract and seaweed oligosaccharides is (1-2):1; and / or the weight ratio of o-cymene-5-ol and tocopheryl retinoate is (1-2):1, the effects of improving acne, repairing and soothing, and improving red blood streaks are better. When the weight ratio of the Camellia chrysantha extract and seaweed oligosaccharides is 1:1; and / or the weight ratio of o-cymene-5-ol and tocopheryl retinoate is 1:1, the effects of improving acne, repairing and soothing, and improving red blood streaks are best.

[0106] From the comparison of Example 1 and Examples 6-7, it can be seen that the extraction method of Camellia chrysantha will affect the effects of improving acne, repairing and soothing, and improving red blood streaks. When the weight ratio of Camellia chrysantha powder to ethanol aqueous solution is 1: (5-10), the effects of improving acne, repairing and soothing, and improving red blood streaks are better.

[0107] From the comparison of Example 1 and Examples 8-10, it can be seen that the type of seaweed oligosaccharide will affect the effects of improving acne, repairing and soothing, and improving red blood streaks. When the seaweed oligosaccharide is seaweed oligosaccharide-III, the effects of improving acne, repairing and soothing, and improving red blood streaks are better.

[0108] From the comparison of Example 1 and Comparative Examples 1-8, it can be seen that the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract of the present invention comprises four components: Camellia chrysantha extract, seaweed oligosaccharides, o-cymene-5-ol, and tocopheryl retinoate, all of which work synergistically and are indispensable.

[0109] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition containing a golden camellia flower extract for anti-pollution and acne-sensitive skin repair, characterized in that: The invention comprises the following components in parts by weight: 1-5 parts of golden camellia extract, 1-5 parts of seaweed oligosaccharide, 0.01-1 parts of o-cymene-5-ol, and 0.01-1 parts of tocopheryl retinoate; The preparation method of the Camellia chrysantha extract comprises the following steps: mixing Camellia chrysantha powder with an ethanol aqueous solution, followed by extraction at 55-65° C. for 25-35 minutes, followed by ultrasonic extraction at 55-65° C. for 55-65 minutes, and then concentrating the extract and drying to obtain the Camellia chrysantha extract; the ethanol aqueous solution comprises 60-80% by volume of ethanol; the weight ratio of the Camellia chrysantha powder to the ethanol aqueous solution is 1:(5-20); the ultrasonic power is 280-300W; and / or the temperature of the concentrated extract is 45-55° C.; The seaweed oligosaccharide is at least one of seaweed oligosaccharide-I, seaweed oligosaccharide-II, seaweed oligosaccharide-III and seaweed oligosaccharide-IV.

2. The anti-pollution and acne-sensitive skin repairing composition containing the Camellia chrysantha extract according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 2-4 parts of golden camellia flower extract, 2-4 parts of seaweed oligosaccharide, 0.05-0.5 parts of o-cymene-5-ol and 0.05-0.5 parts of tocopheryl retinoate.

3. The anti-pollution and acne-sensitive skin repairing composition containing the Camellia chrysantha extract according to claim 1, characterized in that: The weight ratio of the Camellia chrysantha extract and seaweed oligosaccharide is (1-2):

1.

4. The anti-pollution and acne-sensitive skin repairing composition containing the Camellia chrysantha extract according to claim 1, wherein: The weight ratio of o-cymene-5-ol and tocopheryl retinoate is (1-2):

1.

5. Use of the anti-pollution and acne-sensitive skin repairing composition containing the Camellia chrysantha extract according to any one of claims 1 to 4 in the preparation of skin cosmetics.

6. A skin cosmetic, characterized in that: The skin cosmetic comprises the anti-pollution and acne-sensitive skin repairing composition containing the Camellia chrysantha extract according to any one of claims 1 to 4; the skin cosmetic is one of a lotion, an emulsion, a cream, a facial mask, an essence, and a spray.

7. The skin cosmetic according to claim 6, wherein The skin cosmetic comprises the following components in percentage by mass: 1-5% of an anti-pollution and acne-sensitive skin repairing composition containing a Camellia chrysantha extract, 1-40% of a cosmetic base, and the balance being water.

Images