Male anti-hair loss composition containing camellia nitidissima flower extract and application of male anti-hair loss composition

Abstract

The invention relates to a male anti-hair loss composition containing a camellia nitidissima flower extract and application, and belongs to the technical field of cosmetics. The invention firstly provides the anti-hair loss composition containing the camellia nitidissima flower extract for men. The anti-hair loss composition comprises the camellia nitidissima flower extract, a cucumber fruit extract, a gardenia fruit extract and a swertia bimaculata extract in a mass ratio of (0.01-5): (0.5-5): (0.05-0.5): (0.1-2). The composition obtained by compounding the four components in a specific proportion range can synergistically improve the anti-inflammatory and alopecia reducing effects of the composition, and can achieve the purposes of preventing alopecia and growing hair from multiple links such as improving the scalp environment, adjusting the hair follicle cycle and promoting hair follicle autophagy.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a male hair loss prevention composition containing camellia nitidissima h.t. chang flower extract and application. BACKGROUND

[0002] With the acceleration of modern life pace, the increase of mental stress and the intensification of environmental pollution, hair loss problems are increasingly common, especially in the male population. Androgenetic alopecia is the most common type of hair loss, and its pathogenesis is mainly related to androgen levels, hair follicle miniaturization, hair follicle cycle disorder and deterioration of scalp microenvironment.

[0003] The existing hair loss prevention products on the market mostly rely on chemical synthetic ingredients such as minoxidil and finasteride, which have certain effects but have obvious side effects and strong dependence. In addition, some natural plants have also been used in hair loss prevention products, but most of them use the mechanism of inhibiting 5alpha-reductase or androgens to meet the demand for hair loss prevention. However, long-term use of ingredients that inhibit hormones may affect the balance of hormones in the body, and for men, long-term use of ingredients that inhibit androgens may also have some impact on male secondary sexual characteristics. Therefore, the mechanism of inhibiting androgens or 5alpha-reductase to weaken the hair loss phenomenon is not suitable for men, and a more gentle and safe alternative solution is needed. SUMMARY

[0004] The purpose of the present application is to overcome the shortcomings of the prior art and provide a male hair loss prevention composition containing camellia nitidissima h.t. chang flower extract and application.

[0005] To achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows:

[0006] In a first aspect, the present application provides a male hair loss prevention composition containing camellia nitidissima h.t. chang flower extract, which comprises camellia nitidissima h.t. chang flower extract, cucumber fruit extract, gardenia fruit extract and mare's tail extract in a mass ratio of (0.01-5):(0.5-5):(0.05-0.5):(0.1-2).

[0007] The camellia nitidissima h.t. chang flower extract can dredge the scalp capillaries, rapidly activate the hair follicles and promote hair growth. In addition, the polyphenol flavonoid components in the camellia nitidissima h.t. chang flower extract can also relieve inflammatory reactions on the scalp and improve the scalp environment, providing conditions for hair growth.

[0008] The cucumber fruit extract contains active ingredients such as vitamins, amino acids and cucurbitacins. Cucurbitacins can accelerate the transformation of hair follicles from the resting phase to the growth phase by inhibiting the expression of fibroblast growth factor FGF18, effectively promoting hair growth.

[0009] The gardenia fruit extract can down-regulate the expression of the key factor TGF-β1 of hair follicle degradation, and reduce the hair follicle into the involution period; in addition, the gardenia fruit extract can promote the proliferation and secretion of vascular endothelial growth factor VEGF of human dermal papilla cells, and promote hair growth.

[0010] The jujube extract contains oleanolic acid, jujube glycosides and other components, can inhibit apoptosis of cells in hair follicles, reduce hair loss, and also can promote synthesis and secretion of vascular endothelial growth factor of hair follicle cells, stimulate growth of new hair. In addition, studies have shown that the jujube extract can induce autophagy of hair papilla cells, thereby prolonging the growth period of hair follicles and shortening the recession period, achieving the purpose of preventing hair loss.

[0011] The present application selects Camellia nitidissima flower extract, cucumber fruit extract, gardenia fruit extract and Jujube extract for reasonable compounding, and efficacy verification shows that the specific four components in the composition provided by the present application are matched in a specific ratio range, have a significant anti-inflammatory effect, and can synergistically improve the hair loss reduction rate of the subjects in the human anti-hair loss effect test; it is proved that the male anti-hair loss composition containing Camellia nitidissima flower extract provided by the present application can synergistically play a role through specific four components, can achieve the purpose of preventing hair loss and promoting hair growth from improving the scalp environment, regulating the hair follicle cycle, promoting hair follicle autophagy and other aspects, and can effectively avoid the side effects caused by mainly inhibiting androgen or 5α-reductase to weaken hair loss, so it can be used as a more mild and safer alternative solution for preventing and treating hair loss.

[0012] Further, the male anti-hair loss composition containing Camellia nitidissima flower extract includes Camellia nitidissima flower extract, cucumber fruit extract, gardenia fruit extract and Jujube extract with a mass ratio of (0.5-5):(1-5):(0.1-0.5):(0.4-2).

[0013] Preferably, the male anti-hair loss composition containing Camellia nitidissima flower extract includes Camellia nitidissima flower extract, cucumber fruit extract, gardenia fruit extract and Jujube extract with a mass ratio of (0.5-2):(1-2.5):(0.1-0.25):(0.4-1).

[0014] Most preferably, the male anti-hair loss composition containing Camellia nitidissima flower extract includes Camellia nitidissima flower extract, cucumber fruit extract, gardenia fruit extract and Jujube extract with a mass ratio of 0.5:1:0.1:0.4.

[0015] In the second aspect, the present application provides the use of the male anti-hair loss composition containing Camellia nitidissima flower extract in any of the above in the preparation of a male anti-hair loss product.

[0016] Further, the mass ratio of the male anti-hair loss composition containing Camellia nitidissima flower extract in the anti-hair loss product is 1%-5%.

[0017] Preferably, the mass ratio of the male hair loss prevention composition containing the extract of Camellia japonica flowers in the hair loss prevention product is 3%-5%.

[0018] Most preferably, the mass ratio of the male hair loss prevention composition containing the extract of Camellia japonica flowers in the hair loss prevention product is 3%.

[0019] Further, the male hair loss prevention product includes any one of shampoo, shampoo paste, essence or hair conditioner.

[0020] Preferably, the male hair loss prevention product is shampoo.

[0021] In the third aspect, the present application provides a hair loss prevention product containing the male hair loss prevention composition containing the extract of Camellia japonica flowers in any one of the above aspects with a mass ratio of 1-5%.

[0022] Further, the hair loss prevention product further contains auxiliary materials acceptable in the field of cosmetics.

[0023] In the fourth aspect, the present application provides a hair loss prevention shampoo including the following raw materials in the following weight fractions: the male hair loss prevention composition containing the extract of Camellia japonica flowers in any one of the above aspects with a mass ratio of 1-5%, 0.1-0.3% polyquaternium-10, 0.1-0.3% citric acid, 0.1-0.3% disodium EDTA, 3-6% cocamidopropyl betaine, 0.5-1% sodium chloride, 1-3% potassium cocoyl hydrolyzed oat protein, 0.1-0.3% potassium chloride, 1-2% sodium methyl cocoyl taurate, 3-6% cocoyl glucoside, 3-5% sodium C14-16 olefin sulfonate, 0.1-0.3% capryloyl glycine, 0.0.5-0.1% polyquaternium-7, 0.01-0.02% sodium benzoate, 0.5-1% phenoxyethanol, 0.05-0.1% ethylhexylglycerin, and the rest is water.

[0024] Further, the hair loss prevention shampoo includes the following raw materials in the following weight fractions: the male hair loss prevention composition containing the extract of Camellia japonica flowers in any one of the above aspects with a mass ratio of 3%, 0.2% polyquaternium-10, 0.2% citric acid, 0.15% disodium EDTA, 5.4% cocamidopropyl betaine, 0.8% sodium chloride, 1.5% potassium cocoyl hydrolyzed oat protein, 0.2% potassium chloride, 1.55% sodium methyl cocoyl taurate, 5% cocoyl glucoside, 4.2% sodium C14-16 olefin sulfonate, 0.2% capryloyl glycine, 0.09% polyquaternium-7, 0.01% sodium benzoate, 0.72% phenoxyethanol, 0.08% ethylhexylglycerin, and the rest is water.

[0025] Compared with the prior art, the present application has the following beneficial effects:

[0026] The present application provides a male hair loss prevention composition containing camellia japonica flower extract, which comprises camellia japonica flower extract, cucumber fruit extract, gardenia fruit extract and sarracenia purpurea extract in a mass ratio of (0.01-5):(0.5-5):(0.05-0.5):(0.1-2). The composition obtained by compounding the four components in a specific ratio range can synergistically improve its anti-inflammatory and hair loss reduction effects, and can achieve the purposes of preventing hair loss and promoting hair growth from multiple aspects such as improving scalp environment, regulating hair follicle cycle, and promoting hair follicle autophagy. DETAILED DESCRIPTION

[0027] In order to better illustrate the purposes, technical solutions and advantages of the present application, the present application will be further described below in combination with specific examples.

[0028] The camellia japonica flower extract was purchased from Ximu Source (Xiamen) Biotechnology Co., Ltd., and the trade name was camellia japonica flower extract;

[0029] The cucumber fruit extract was purchased from Lipotec, and the trade name was Actismart TM SW cucumber;

[0030] The gardenia fruit extract was purchased from Guangzhou Baolong International Trade Co., Ltd., and the trade name was gardenia fruit extract;

[0031] The sarracenia purpurea extract was purchased from Fufeng Ciyuan Biotechnology Co., Ltd., and the trade name was sarracenia purpurea extract.

[0032] Other materials, reagents, etc. used in the examples can be obtained from commercial channels if not specifically stated.

[0033] Examples 1-4 and Comparative Examples 1-6

[0034] According to the formula in Table 1, each component was weighed and mixed to obtain the compositions of application examples 1-4 and comparative application examples 1-6. The camellia japonica L. flower extract was purchased from Shaanxi Xintianyuan Biotechnology Co., Ltd.

[0035] Table 1 Components and mass parts of the composition

[0036]

[0037] Note: “-” represents no addition; and the total mass of the composition in different examples and comparative examples is the same.

[0038] Test Example 1

[0039] The compositions of examples 1-4 and comparative examples 1-6 were used as test samples for efficacy determination.

[0040] (1) Inhibition of 5α-reductase by the composition

[0041] The experimental method is as follows:

[0042] The compositions of each example and comparative example were respectively prepared into a PBS solution with a concentration of 0.2 g / 100 mL as a sample liquid.

[0043] Sample tube: 1 mL of different sample liquid, 1 mL of enzyme liquid (0.2 mg / mL), 1 mL of NADPH solution (1 mmol / L) and 1 mL of testosterone solution (1 mmol / L) were added into a test tube, and shaken gently, 200 μL was taken into a 96-well enzyme plate with a pipette gun, three parallels were made for each sample, and placed into an enzyme marker instrument for detection, and the absorbance value at 340 nm was determined as the first determination value A 样品0 . After being placed into a 37°C incubator for 20 minutes, the enzyme marker instrument was detected again, and the absorbance value at 340 nm was determined as the second determination value A 样品20 .

[0044] Enzyme tube: 1 mL of PBS solution, 1 mL of enzyme liquid (0.2 mg / mL), 1 mL of NADPH solution (1 mmol / L) and 1 mL of testosterone solution (1 mmol / L) were added into a test tube, and shaken gently, 200 μL was taken into a 96-well enzyme plate with a pipette gun, three parallels were made, and placed into an enzyme marker instrument for detection, and the absorbance value at 340 nm was determined as the first determination value A 酶0 , After being placed into a 37°C incubator for 20 minutes, the enzyme marker instrument was detected again, and the absorbance value at 340 nm was determined as the second determination value A enzyme 20. The inhibition rate of the enzyme was calculated according to the following formula:

[0045]

[0046] (2) Test of anti-inflammatory ability of the composition

[0047] The experimental method is as follows:

[0048] Plating: logarithmic growth phase RAW264.7 cells were inoculated in a 24-well plate, with a cell density of 2 x 10 5 mL per well, and the plate was plated and labeled on the cover of the cell plate after plating, and placed into a carbon dioxide incubator for incubation for 24 h;

[0049] Sample treatment: after the cells were incubated for 24 h, the supernatant was removed, and the following samples 0.5 mL were added respectively: ① negative control group: 1% FBS + DMEM medium; ② model group: LPS (1 μg / mL) + 1% FBS + DMEM medium; ③ experimental group: 0.1% composition of each example or comparative example + LPS (1 μg / mL) + 1% FBS + DMEM medium.

[0050] Detection of inflammatory factors in cell supernatant: After treating the cells for 24 h, the supernatant was sucked into a centrifuge tube with a pipette, centrifuged at 12,000 g at 4°C for 5 min, and the precipitate was discarded. The content of inflammatory factors IL-6 and NO was detected by an ELISA kit; the corresponding inhibition rate was calculated, and the inhibition rate % = (model group inflammatory factor amount-experimental group inflammatory factor amount) / model group inflammatory factor amount * 100%; the higher the inhibition rate, the stronger the anti-inflammatory ability.

[0051] Test results:

[0052] As shown in Table 2, the compositions of the examples and the comparative examples have a certain inhibitory effect on 5α-reductase, but the effect is limited, indicating that the compositions of the present application do not mainly exert the anti-hair loss effect by inhibiting 5α-reductase. As for the anti-inflammatory effect of the compositions, the NO inhibition rate and the IL-6 inhibition rate of the compositions of Examples 1-4 are significantly higher than those of the compositions provided in the comparative examples. Specifically, comparing Example 2 with Comparative Examples 1-5, it can be seen that there is a synergistic effect among the four components of the extract of L. japonica flower, the extract of C. pepo fruit, the extract of G. japonica fruit, and the extract of N. indicum in terms of anti-inflammatory effect, and the absence of one of the components or the replacement of the other component with similar efficacy will cause the anti-inflammatory effect of the composition to be greatly reduced. Comparing the results of Example 2 and Comparative Example 6, it can be seen that the ratio of the compositions also has an important influence on the anti-inflammatory effect.

[0053] Table 2 Efficacy determination results of the compositions

[0054]

[0055] Application Examples 1-4 and Comparative Application Examples 1-6

[0056] The compositions of Examples 1-4 and Comparative Examples 1-6 are used to prepare anti-hair loss shampoos, obtaining the corresponding anti-hair loss shampoos of Application Examples 1-4 and Comparative Application Examples 1-6.

[0057] According to Table 3, the corresponding raw materials and amounts are weighed, and the preparation process of the anti-hair loss shampoo is as follows:

[0058] (1) Polyquaternium-10 is added to deionized water and stirred to disperse. After uniform dispersion, the other materials of phase A are added. After heating to 85°C, the mixture is stirred and dissolved uniformly. Then, it is kept warm for standby, obtaining a pre-prepared phase A;

[0059] (3) At 80-85°C, each component of phase B is added, and the mixture is stirred and dissolved uniformly until transparent. Then, it is kept warm to defoam;

[0060] (3) The temperature is lowered to below 45°C, and the raw materials of phase C are added one by one and stirred uniformly;

[0061] (4) continue to add the raw materials of phase D (dissolve the composition in water in advance), mix well, and the male anti-hair loss shampoo is obtained.

[0062] Table 3: Raw material table of the male anti-hair loss shampoo

[0063]

[0064] Test Example 2: Human anti-hair loss effect test of the anti-hair loss product

[0065] The anti-hair loss shampoos of application examples 1-4 and comparative application example 1-6 are used as test samples

[0066] 100 male volunteers aged 18-45 years old with different degrees of hair loss problems are selected and divided into 10 groups, 10 people in each group. The test subjects in different groups use the anti-hair loss shampoos of the above application examples or comparative application example once a day for washing hair, and the amount used each time is 5 mL. After 8 weeks and 12 weeks of use, the hair loss count is carried out, 60 times of combing (using a standard non-static comb by a professional), and the number of fallen hair after 60 times of combing is counted. The number of hair loss before use and after 8 weeks and 12 weeks of use is counted respectively. The test subjects do not wash their hair within 48±4 hours before each visit, and do not comb their hair before the hair loss count on the day of visit. The hair loss reduction rate is calculated, and the hair loss reduction rate % = (the number of hair loss at 0 weeks - the number of hair loss at 8 / 12 weeks) / the number of hair loss at 0 weeks;

[0067] The test results are shown in Table 4. Application example 1-4 shows a good effect of reducing hair loss after 8 weeks of use, and the anti-hair loss effect is further improved after 12 weeks of continuous use. The anti-hair loss effects of comparative application example 1-6 after 8 weeks and 12 weeks of use are not only significantly poorer than those of application example 1-4, but also show no further improvement trend when the use time is extended from 8 weeks to 12 weeks. The results of application example 2 and comparative application example 1-6 show that the specific proportions of Luvosca flower extract, cucumber fruit extract, gardenia fruit extract and Potentilla anserina extract in the composition can synergistically improve the hair loss of the test subjects. In combination with the 5α-reductase inhibition results of the composition in test example 1, it can be known that the composition provided in the application can achieve the purposes of preventing hair loss and promoting hair growth from multiple aspects such as improving the scalp environment, regulating the hair follicle cycle, and promoting hair follicle autophagy, instead of mainly inhibiting 5α-reductase to prevent hair loss. Therefore, the composition provided in the application can be a safer and milder alternative to inhibiting androgens or 5α-reductase to weaken the hair loss phenomenon, and can alleviate the disadvantages caused by long-term use of androgen-inhibiting components by men.

[0068] Table 4: Human anti-hair loss effect test results

[0069]

[0070]

[0071] Finally, it should be pointed out that the above examples are only used to illustrate the technical solutions of the present application and are not intended to limit the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A male hair loss prevention composition comprising a Camellia japonica flower extract, characterized in that, It includes extracts of Camellia chrysantha flower, cucumber fruit, gardenia fruit, and swertia shoots in a mass ratio of (0.01-5):(0.5-5):(0.05-0.5):(0.1-2).

2. The men's hair loss prevention composition containing extract of V. patula flowers according to claim 1, wherein It includes extracts of Camellia chrysantha flower, cucumber fruit, gardenia fruit, and swertia shoots in a mass ratio of (0.5-5):(1-5):(0.1-0.5):(0.4-2).

3. The men's hair loss prevention composition containing extract of V. fordii flowers according to claim 1, wherein It includes extracts of Camellia chrysantha flower, cucumber fruit, gardenia fruit, and swertia shoots in a mass ratio of (0.5-2):(1-2.5):(0.1-0.25):(0.4-1).

4. The use of the male anti-hair loss composition containing Camellia japonica flower extract as described in any one of claims 1-3 in the preparation of male anti-hair loss products.

5. The use according to claim 4, wherein the compound is ###0002### The male anti-hair loss composition containing Camellia japonica flower extract accounts for 1%-5% of the total weight of the anti-hair loss product.

6. Use according to claim 5, wherein The male anti-hair loss composition containing Camellia japonica flower extract accounts for 3%-5% of the total weight of the anti-hair loss product.

7. The use according to claim 4, wherein the compound is ###0002### The men's anti-hair loss products include any one of shampoo, hair cream, serum, or conditioner.

8. A hair loss prevention product characterized by comprising: A male anti-hair loss composition containing Camellia japonica extract as described in any one of claims 1-3, at a mass ratio of 1-5%.

9. The hair loss prevention product according to claim 8, wherein It also contains excipients acceptable in the cosmetics industry.

10. An anti-hair loss shampoo, characterized by, The composition comprises the following ingredients by weight fraction: 1-5% of the male anti-hair loss composition containing Camellia japonica flower extract as described in any one of claims 1-3, 0.1-0.3% polyquaternium-10, 0.1-0.3% citric acid, 0.1-0.3% disodium EDTA, 3-6% cocamidopropyl betaine, 0.5-1% sodium chloride, 1-3% potassium cocoyl hydrolyzed oat protein, 0.1-0.3% potassium chloride, 1-2% sodium methylcocoyl taurate, 3-6% cocoyl glucoside, 3-5% sodium C14-16 olefin sulfonate, 0.1-0.3% capryloyl glycine, 0.05-0.1% polyquaternium-7, 0.01-0.02% sodium benzoate, 0.5-1% phenoxyethanol, 0.05-0.1% ethylhexylglycerin, and the balance being water.

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