A Corydalis yanhusuo and Angelica dahurica essence nano-fiber membrane, its preparation method and application

Abstract

The present invention relates to a Corydalis yanhusuo and Angelica dahurica essence nano - fiber membrane, its preparation method and application. The preparation method includes: adding N - vinylpyrrolidone to Corydalis yanhusuo and Angelica dahurica essence with a mass ratio of 2 - 3:1; then adding a mixed solvent of water and ethanol, adjusting the pH to 6.0, and further adding poloxamer and mixing evenly to obtain a Corydalis yanhusuo and Angelica dahurica essence electrospinning solution; and preparing the Corydalis yanhusuo and Angelica dahurica essence nano - fiber membrane by the high - voltage electrospinning method. Under the action of poly(N - vinylpyrrolidone), poloxamer, etc., the Corydalis yanhusuo and Angelica dahurica essence in the present invention is transformed into dry - state nano - fibers through the high - voltage electrospinning method to obtain a nano - fiber membrane, which has characteristics such as being porous, soft, and having a large specific surface area. It can quickly adsorb on the nasal cavity, oral mucosa or skin surface and rapidly release the essence components, which can be effectively absorbed by the mucosa and skin surface, increasing the bioavailability of the plant essence components. It can be applied to preparations for treating metabolic diseases such as anti - depression, analgesia, diabetes, smoking and alcohol cessation, or sleep aid, etc.

Description

Technical Field

[0001] The present invention belongs to the field of biomedicine, and particularly relates to a Corydalis yanhusuo and Angelica dahurica extract nanometer fiber membrane, a preparation method thereof and an application thereof. Background Art

[0002] Corydalis yanhusuo, also known as Rhizoma Corydalis, Radix Aconiti, Rhizoma Corydalis, belongs to the Papaveraceae family of Chinese medicinal plants. Its medicinal part is the rhizome extending on the underground stem. Corydalis yanhusuo has been widely used in traditional Chinese medicine for analgesia, sedation, expelling wind and activating blood circulation since ancient times. Corydalis yanhusuo contains a variety of active ingredients, and the main components include tetrahydropalmatine, corydaline, fumaric acid, etc. These components are considered to have effects such as analgesia, sedation, anti-inflammatory, expelling wind and activating blood circulation. Among them, tetrahydropalmatine is considered to be the main component of the analgesic effect of Corydalis yanhusuo. The analgesic mechanism of Corydalis yanhusuo may involve multiple aspects such as anti-inflammatory effects, regulation of the central nervous system, influence on drug active sites, interaction of the endogenous pain regulation system, and influence on nerve conduction pathways. Clinically, Corydalis yanhusuo is still widely used in analgesic treatment, including rheumatic pain, headache, dysmenorrhea, visceral pain, pain caused by trauma such as sprain and contusion, and some cancer pain. In recent years, scientific research has also shown that Corydalis yanhusuo also plays an important role in the treatment of diseases in the fields of neuropsychiatry, cardiovascular system, endocrine metabolism and drug addiction.

[0003] Angelica dahurica: It is white in color, warm in nature, pungent in taste and slightly bitter. It has the effects of dispersing wind and removing dampness, dredging orifices and relieving pain, and detumescence and discharging pus. Clinically, it is widely used in diseases such as common cold headache, supraorbital pain, nasal congestion, nasal sinusitis, toothache, leukorrhea, abscess, etc., and has good curative effects. One of the main components of Angelica dahurica is coumarin compounds, which have a dilating effect on coronary artery blood vessels and also have an antagonistic effect on calcium ions. Therefore, it can be used to treat hypertension. Moreover, isoimperatorin contained in Angelica dahurica has the effect of promoting blood circulation and removing stasis, and can achieve the function of preventing cardiovascular and cerebrovascular diseases. In addition, coumarin components contained in Angelica dahurica, such as xanthotoxin, imperatorin and oxypeucedanin hydrate, etc., can inhibit the effect of insulin-induced triglyceride synthesis from glucose, thereby indirectly playing a role in promoting fat decomposition and inhibiting fat synthesis. Summary of the Invention

[0004] The purpose of the present invention is to provide a Corydalis yanhusuo and Angelica dahurica extract nanometer fiber membrane, a preparation method thereof and an application thereof, so as to solve the problems existing in the prior art.

[0005] A preparation method of a Corydalis yanhusuo and Angelica dahurica extract nanometer fiber membrane comprises the following steps:

[0006] (1) Respectively prepare Corydalis yanhusuo extract and Angelica dahurica extract;

[0007] (2) Add the Corydalis yanhusuo extract and Angelica dahurica extract to an N-vinylpyrrolidone solution according to a mass ratio of (2-3):1;

[0008] (3) Then add the mixed solvent of water and ethanol and adjust the pH to 6.0;

[0009] (4) Then add poloxamer and mix evenly at room temperature to obtain the electrostatic spinning solution of Corydalis yanhusuo and Angelica dahurica extract;

[0010] (5) Prepare the nano - fiber membrane of Corydalis yanhusuo and Angelica dahurica extract by using the high - voltage electrostatic spinning method for the electrostatic spinning solution of Corydalis yanhusuo and Angelica dahurica extract.

[0011] Further, the process parameters of the high - voltage electrostatic spinning method are as follows: the feeding speed of the electrostatic spinning solution of Corydalis yanhusuo and Angelica dahurica extract is 1.5 - 3 mL / h, the roller rotation speed is 200 - 400 RPM, and the spinning time is 2 - 4 hours. More preferably, adjust the electrostatic spinning process parameters as follows: (1) the feeding speed of the spinning solution is 1.5 mL / h on an electrostatic spinning machine with a single - needle head for spinning, (2) the size of the spinneret needle of the spinning solution is 21G, (3) the electric field strength is 18 kV, (4) the fiber collection distance is 15 cm, (5) the temperature is room temperature, the environmental relative humidity is 45%, and (6) the spinning time is two hours.

[0012] Further, the mass content of N - vinylpyrrolidone in the electrostatic spinning solution of Corydalis yanhusuo and Angelica dahurica extract is 45 - 50%, the mass content of Corydalis yanhusuo and Angelica dahurica extract is 9 - 10%, the mass content of poloxamer is 0.1 - 0.12%, and the viscosity at room temperature is 350 - 650 cP.

[0013] Further, the molecular weight of N - vinylpyrrolidone is 43000 - 50000 g / mol.

[0014] Further, the mass ratio of water to ethanol in the mixed solvent of water and ethanol is 90:10. When the mixed solvent with a mass ratio of water to ethanol of 90:10 is selected, the dissolution rate of fat - soluble substances in Corydalis yanhusuo and Angelica dahurica is the highest and the medicinal effect is the strongest.

[0015] Add N - vinylpyrrolidone (molecular weight 43000 g / mol) to Corydalis yanhusuo and Angelica dahurica extract (2:1). It has good biocompatibility, bio - absorbability, and aqueous solution spinnability, and is used as the matrix polymer to realize the electrostatic spinning process. Add a certain proportion of the water - ethanol solvent mixture (mass ratio 90 / 10), adjust the pH to 6.0, add poloxamer F - 127, and mix evenly to obtain a homogeneous solution at room temperature. The mass concentration of the polymer is 45%, the mass concentration of Corydalis yanhusuo and Angelica dahurica extract is 9%, the mass concentration of poloxamer is 0.1%, and the ethanol solvent mixture is 45.9%. The viscosity at room temperature is 350 cP.

[0016] Poloxamer F-127 is an efficient surfactant that plays two roles in the nanofilm: 1) It makes Yuanhu Zhenlu more evenly distributed and more stable; 2) It helps with penetration.

[0017] The Yuanhu Zhenlu of the present invention has the best solubility under slightly acidic conditions, especially when the pH value is 6.

[0018] The Yuanhu Baizhi Zhenlu nanofiber film obtained by the above preparation method. This film sheet loads all the effective components of Zhenlu and is a loose and porous nanofiber film sheet with uniform fiber size. The film layer is soft and easy to apply on the nasal mucosa, oral mucosa or skin surface.

[0019] A micro-patch comprising the Yuanhu Baizhi Zhenlu nanofiber film prepared above. The micro-patch includes a nasal mucosa micro-patch, an oral mucosa micro-patch and a skin micro-patch.

[0020] The application of the above Yuanhu Baizhi Zhenlu nanofiber film in the preparation of antidepressant preparations.

[0021] The application of the above Yuanhu Baizhi Zhenlu nanofiber film in the preparation of preparations for treating insulin-related metabolic diseases.

[0022] The application of the above Yuanhu Baizhi Zhenlu nanofiber film in the preparation of preparations for quitting smoking or alcohol or addiction.

[0023] The application of the above Yuanhu Baizhi Zhenlu nanofiber film in the preparation of analgesics and hypnotics.

[0024] With the help of the intermolecular hydrogen bond between the polar groups of the polymer poly(N-vinylpyrrolidone) and the molecules on the surface of the mucosa, this nanofiber film is easily fitted and adhered to the surface of the nasal mucosa or oral mucosa. Under the conventional humidity provided during nasal breathing, the film sheet absorbs a small amount of moisture on the surface of the nasal mucosa and gradually dissolves. The small molecule effective essence components of Yuanhu and Baizhi Zhenlu released are quickly absorbed by the nasal mucosa and quickly act on the blood vessels in the brain through the nasal-brain pathway. At the same time, the water-soluble polymer part is absorbed, degraded and excreted by the body, and the rest leaves the nasal cavity along with the nasal mucosa secretions. Therefore, loading the effective components of Yuanhu Baizhi Zhenlu on the nanofiber film is a new drug delivery technology, which is skillfully applied to the nasal mucosa and absorbed by the body through the mucosal layer, avoiding the first-pass effect of the liver and used for preventing and treating various diseases.

[0025] Compared with the prior art, the Yuanhu Baizhi Zhenlu nanofiber film of the present invention mainly has the following remarkable advantages:

[0026] (1) The nanofiber film can effectively load all the components of the essence of Yuanhu and Baizhi Zhenlu. In particular, the alkaloid components that play an important role in medical care can be dissolved in an acidic mixed solution mainly composed of water and ethanol.

[0027] (2) During the electrospinning process at room temperature, the Yuanhu Baizhi Zhenlu electrospinning solution is directly converted into dry nanofibers, so that it is uniformly retained in the main polymer matrix of the nanofibers; the nanofiber membrane is extremely porous and has a very large specific surface area, which greatly increases the effective contact area with the nasal mucosa surface, can quickly adsorb on the nasal mucosa surface and rapidly release the essence components, and is effectively absorbed by the nasal mucosa surface, increasing the bioavailability of the Zhenlu components; the overall nanofiber membrane is soft and has a high porosity, and is easy to fit and adhere to the nasal mucosa, oral mucosa or skin surface.

[0028] (3) The polar polymer poly(N-vinylpyrrolidone) has the adhesion ability to quickly adhere to the mucosa and skin. The matrix polymers all have good spinnability in aqueous solution, are used as matrix polymers to realize the electrospinning process, and have good adhesion to the oral and nasal mucosa.

[0029] (4) Add the commonly used commercial poloxamer F-127 in the food industry and biomedicine as a penetration enhancer, which helps to promote the penetration of the Zhenlu essence components released from the fiber membrane patch through the oral or nasal mucosa layer and be absorbed.

[0030] (5) A mild and environmentally friendly aqueous solution system is used in the preparation process of the nanofiber membrane, and a small amount of ethanol is used. The whole process is safe, non-toxic, environmentally friendly, and can retain all the effective components of the Zhenlu essence. Description of the Drawings

[0031] Figure 1 is the SEM image of the nanofiber membrane prepared by the present invention;

[0032] Figure 2 is the influence diagram of the Yuanhu Baizhi Zhenlu nanofiber membrane of the present invention on the pain threshold of mice. Among them, (A) is the measurement of the skin pain threshold of mice by the hot plate method, (B) is the measurement of the visceral pain threshold by the acetic acid writhing method, and (C) is the pain response of the pain model mice measured by the ciliary method;

[0033] Figure 3 is the influence diagram of the Yuanhu Baizhi Zhenlu nanofiber membrane of the present invention on the depressive-like behavior of chronic depression model mice. Among them, (A) is the open field movement distance, (B) is the immobile time during forced swimming, and (C) is the immobile time during tail suspension determination;

[0034] Figure 4 is the influence of the Yuanhu Baizhi Zhenlu nanofiber membrane of the present invention on insulin resistance (A), blood glucose (B), insulin (C) and blood lipids (D) of diabetic model mice, as well as the body weight of the model mice after 10 days of treatment (E), and the pathological changes of adipocytes and myocytes (F);

[0035] Figure 5It is a diagram showing the effects of the Corydalis yanhusuo and Angelica dahurica Zhenlu nanofiber membrane of the present invention on nicotine and alcohol addiction behaviors in mice. Among them, (A) is nicotine-induced, and (B) is alcohol-induced place preference response.

[0036] Figure 6 It is the effect of oral mucosal application of the Corydalis yanhusuo and Angelica dahurica Zhenlu nanofiber membrane of the present invention on the sleep of mice; in the figure, (A) is the control group of saline nanofiber membrane, (B) is the group of Corydalis yanhusuo and Angelica dahurica Zhenlu nanofiber membrane, and (C) is the performance comparison between the saline nanofiber membrane for 4 minutes and the Corydalis yanhusuo and Angelica dahurica Zhenlu nanofiber membrane for 4 minutes. Detailed implementation manners

[0037] To make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings.

[0038] Example 1

[0039] A preparation method of a Corydalis yanhusuo and Angelica dahurica Zhenlu nanofiber membrane includes the following steps:

[0040] (1) Prepare an electrospinning solution. Prepare a solution of poly(N-vinylpyrrolidone) (molecular weight: 43000 g / mol), add Corydalis yanhusuo Zhenlu and Angelica dahurica Zhenlu with a mass ratio of 2:1, the solvent is a mixture of water and ethanol (90 / 10 mass ratio), and adjust the pH value to 6.0 with citric acid. Add poloxamer F-127 and mix evenly to obtain a homogeneous solution at room temperature. The mass concentration of the polymer poly(N-vinylpyrrolidone) in the obtained electrospinning solution is 45%, the mass concentration of Corydalis yanhusuo and Angelica dahurica Zhenlu is 9%, the mass concentration of poloxamer is 0.1%, and the viscosity at room temperature is 350 cP.

[0041] (2) Prepare a nanofiber membrane by the high-voltage electrospinning method.

[0042] Use an electrospinning machine. At room temperature, use this solution as the electrospinning solution. In the high-voltage electrospinning process on a single-needle spinning electrospinning machine, the feeding speed of the spinning solution is 1.5 mL / h, the spinning solution spraying needle head is 21G in size, the electric field strength is 18 kV, the fiber collection distance is 15 cm, the temperature is room temperature, and the environmental relative humidity is 45%. Use aluminum foil as the receiving substrate on the surface of the roller to receive the nanofiber membrane, and the roller rotation speed is 200 RPM. Spin for two hours to obtain a dry, loose and porous nanofiber membrane sheet. The obtained nanofiber membrane sheet is loaded with all the effective components of Zhenlu, and the fiber size is uniform. It is observed under an electron microscope that the fiber diameter is 900 nanometers, the nanofiber membrane is loose and porous, the overall thickness is 100 microns, and the membrane layer is soft and easy to apply on the nasal mucosa surface. Figure 1The SEM photos show the microscopic morphology of the nanofiber membrane. The diameters of the nanofibers are uniform, about 900 nm, and the Zhenlu essence components are loaded in the fibers of the polymer matrix.

[0043] Example 2

[0044] A preparation method of Corydalis yanhusuo and Angelica dahurica Zhenlu nanofiber membrane includes the following steps:

[0045] (1) Prepare the electrospinning solution. Prepare a solution of poly(N-vinylpyrrolidone) (molecular weight: 43000 g / mol), add Corydalis yanhusuo Zhenlu and Angelica dahurica Zhenlu with a mass ratio of 2:1, the solvent is a mixture of water and ethanol (mass ratio 90 / 10), and adjust the pH value to 6.0 with citric acid. Add poloxamer F-127 and mix evenly to obtain a homogeneous solution at room temperature. In the obtained electrospinning solution, the mass concentration of the polymer poly(N-vinylpyrrolidone) is 50%, the mass concentration of Corydalis yanhusuo and Angelica dahurica Zhenlu is 10%, the mass concentration of poloxamer is 0.12%, and the viscosity at room temperature is 650 cP.

[0046] (2) Prepare the nanofiber membrane by the high-voltage electrospinning method.

[0047] Use an electrospinning machine. At room temperature, using this solution as the electrospinning solution, perform the high-voltage electrospinning process on a single-needle electrospinning machine. The feeding speed of the electrospinning solution is 1.5 mL / h, the nozzle of the electrospinning solution is 21G in size, the electric field strength is 18 kV, the fiber collection distance is 15 cm, the temperature is room temperature, and the environmental relative humidity is 45%. Use aluminum foil as the receiving substrate on the surface of the roller to receive the nanofiber membrane, and the roller speed is 200 RPM. Spin for two hours to obtain a dry, loose and porous nanofiber membrane sheet. The obtained nanofiber membrane sheet loads all the effective components of Zhenlu, and the fiber sizes are uniform. Under the electron microscope, the fiber diameter is observed to be 900 nm. The nanofiber membrane is loose and porous, with an overall thickness of 120 microns, and the membrane layer is soft and easy to adhere to the nasal mucosa surface.

[0048] Performance test and data

[0049] 1. Influence of Corydalis yanhusuo and Angelica dahurica Zhenlu (abbreviated as Yuanbai Zhenlu) nanofiber membrane on the pain threshold of mice

[0050] Place the prepared tiny Yuanbai Zhenlu nanofiber membrane (1x1 mm) in the nasal cavity of C57BL / 6 mice for 2 - 4 minutes, and then detect the skin pain threshold of the mice (hot plate method, Figure 2 in (A)) and the visceral pain threshold (acetic acid writhing method, Figure 2 in (B), as shown in the figure). Compared with the control group (saline membrane), the Yuanbai Zhenlu nanofiber membrane treatment significantly increased the pain threshold, reaching the level of the positive control drug (lidocaine), and the effect was better than that of intragastric administration of Yuanbai Zhenlu. Figure 2In (C), the effects of Yuanbai Zhenlu nanofiber membrane applied to the plantar surface of pain model mice on pain response were detected. The results showed that compared with the control group (solvent group), the Yuanbai Zhenlu nanofiber membrane significantly reduced the pain response of mice (i.e., enhanced the analgesic effect) 10 and 30 seconds after application.

[0051] 2. Effects of Yuanbai Zhenlu nanofiber membrane on depressive behaviors in depressive model mice

[0052] The prepared tiny Yuanbai Zhenlu nanofiber membrane (1x1 mm) was placed in the nasal cavity of C57BL / 6 mice for 2 minutes, and then the depressive-like behaviors of chronic depressive model mice were detected, including the distance of movement in the open field, the immobility time during forced swimming, and the immobility time during tail suspension test. The experimental results are as Figure 3 shown. The treatment with Yuanbai Zhenlu nanofiber membrane significantly improved the depressive-like behaviors of depressive model mice, and the effect of Yuanbai Zhenlu nanofiber membrane was better than that of treating with Corydalis yanhusuo membrane or Angelica dahurica membrane alone, and also better than that of intragastric administration of Yuanbai Zhenlu. It is indicated that Yuanbai Zhenlu nanofiber membrane has a good anti-depressant effect.

[0053] 3. Effects of Yuanbai Zhenlu nanofiber membrane on insulin resistance, blood glucose, blood lipids and body weight in diabetic model mice

[0054] The prepared tiny Yuanbai Zhenlu nanofiber membrane (1x1 mm) was placed in the nasal cavity of one side of high-sugar / high-fat induced insulin resistance model rats (SD) for 2 minutes per day. After 10 days of treatment, the insulin resistance index, blood glucose, blood insulin and blood lipid levels were detected. The results are as Figure 4 shown. The treatment with Yuanbai Zhenlu nanofiber membrane for 1 week could significantly improve insulin resistance, reduce blood glucose, blood insulin and blood lipids. In addition, as Figure 4 shown in (E), the body weight of model mice significantly decreased after 10 days of treatment with Yuanbai Zhenlu nanofiber membrane; and pathological examination found that the lesions of liver cells, adipocytes and muscle cells in model mice were all improved (as Figure 4 shown in (F)). The experimental results indicate that in insulin resistance model mice, the treatment with Yuanbai Zhenlu nanofiber membrane shows obvious effects of improving insulin resistance, reducing blood glucose, blood lipids and body weight.

[0055] 4. Effects of Yuanbai Zhenlu nanofiber membrane on nicotine and alcohol addictive behaviors in mice

[0056] In the conditioned place preference behavior experiment of mice induced by nicotine or alcohol, after the prepared tiny Yuanbai Zhenlu nanofiber membrane (1x1 mm) was placed in the nasal cavity of C57BL / 6 mice for 2 minutes, nicotine (0.5 kg / kg) or alcohol (2 g / kg) was intraperitoneally injected, and then the nicotine and alcohol addictive behaviors (place preference behavior) of mice were detected respectively. The results are as Figure 5As shown, simultaneous treatment with Yuanbai Zhenlu nanofiber membrane and nicotine or alcohol can prevent the formation of nicotine and alcohol place preference behavior, and the effect of Yuanbai Zhenlu nanofiber membrane is better than that of intragastric administration of Yuanhu Zhenlu. The experimental results show that treatment with Yuanbai Zhenlu nanofiber membrane has the effect of assisting in smoking cessation and alcohol abstinence.

[0057] 5. Effect of oral mucosal treatment with Yuanbai Zhenlu nanofiber membrane on sleep in mice

[0058] C57BL / J6 mice were surgically implanted with electroencephalogram recording electrodes, and then the electroencephalogram and electromyogram of freely moving mice were recorded. After 10 minutes of oral mucosal treatment with Yuanbai Zhenlu nanofiber membrane, sleep waves appeared in the electroencephalogram of mice and the electromyogram activity was significantly weaker ( Figure 6 in (B)), indicating that compared with the control group (saline, Figure 6 in (A)), oral mucosal treatment with Yuanbai Zhenlu nanofiber membrane can promote sleep. As can be seen from Figure 6 in (C), the proportion of rapid eye movement (REM) sleep in the control group was much larger than that of non-REM sleep; the proportion of REM sleep in the Yuanbai nanofiber membrane group was less than that of non-REM sleep; indicating that the Yuanbai nanofiber membrane has a sleep-promoting effect.

[0059] Comparative Example 1

[0060] The mass ratio of Yuanhu and Baizhi Zhenlu added in Example 1 was changed to 1:1, and the others remained unchanged. It was found that the sleep-promoting effect would be weakened.

[0061] Comparative Example 2

[0062] Poloxamer F-127 was not added, and the others were the same as in Example 1. It was found that the comprehensive effect of the obtained Yuanbai Zhenlu nanofiber membrane was reduced.

[0063] Comparative Example 3

[0064] The pH in Example 1 was changed to 7.0, and the others remained unchanged. The solubility of Yuanhu in the obtained electrospinning solution was lower.

[0065] Comparative Example 4

[0066] The water and ethanol mixture (90 / 10 mass ratio) in Example 1 was changed to water, and the others remained unchanged. It was found that the dissolution rate of fat-soluble substances in Yuanhu and Baizhi decreased and the drug effect weakened.

Claims

1. A method for preparing a Yuanhu Baizhi Zhenlu nanofiber membrane, characterized in that: The following steps are involved: (1) preparing Yuanhu Zhenlu and Baizhi Zhenlu respectively; (2) adding Corydalis yanhusuo and Angelica dahurica extract into the poly(N-vinyl pyrrolidone) solution at a mass ratio of (2-3):1; (3) then adding a mixed solvent of water and ethanol and adjusting the pH to 6.0; (4) adding poloxamer and mixing evenly at room temperature to obtain Yuanhu Baizhi Zhenlu electrospinning solution; (5) preparing the Yuanhu Baizhi Zhenlu nanofiber membrane by using the Yuanhu Baizhi Zhenlu electrospinning solution through a high-voltage electrospinning method; The viscosity of the Yuanhu Baizhi Zhenlu electrospinning solution at room temperature is 350-650 cP, the mass content of poly(N-vinyl pyrrolidone) is 45-50%, the total mass content of Yuanhu and Baizhi Zhenlu is 9-10%, the mass content of poloxamer is 0.1-0.12%, and the molecular weight of the poly(N-vinyl pyrrolidone) is 43000-50000 g / mol; The feeding rate of Yuanhu Baizhi Zhenlu electrospinning solution is 1.5-3mL / h, the drum speed is 200-400 RPM, and the spinning time is 2-4 hours; The mass ratio of water to ethanol in the mixed solvent is 90:

10.

2. The Yuanhu Baizhi Zhenlu nanofiber membrane obtained according to the preparation method described in claim 1.

3. A micro patch, characterized in that: It comprises the Yuanhu Baizhi Zhenlu nanofiber membrane according to claim 2, and the micro-patch includes a nasal mucosa micro-patch and an oral mucosa micro-patch.

4. Use of the Yuanhu Baizhi Zhenlu nanofiber membrane according to claim 2 in the preparation of antidepressant or sleep-aiding preparations.

5. Use of the Yuanhu Baizhi Zhenlu nanofiber membrane according to claim 2 in the preparation of a preparation for treating diabetes.

6. Use of the Yuanhu Baizhi Zhenlu nanofiber membrane according to claim 2 in preparing smoking cessation or alcohol cessation preparations.

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