Traditional Chinese medicine composition for treating xerophthalmia and asthenopia as well as preparation method and application thereof

Abstract

The invention belongs to the field of traditional Chinese medicines, and relates to a traditional Chinese medicine composition for treating xerophthalmia and asthenopia and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 45 to 55 parts of flos chrysanthemi indici, 35 to 45 parts of radix salviae miltiorrhizae, 8 to 12 parts of fructus viticis, 8 to 12 parts of spica prunellae, 4 to 8 parts of flos buddlejae, 10 to 20 parts of semen cassiae, 4 to 8 parts of rhizoma coptidis, 5 to 10 parts of cortex fraxini, 5 to 8 parts of anoectochilus roxburghii, 10 to 15 parts of cortex dictamni, 0.1 to 0.5 part of menthol, 0.1 to 0.3 part of borneol, 3 to 5 parts of enzymolysis pearl powder, 2 to 5 parts of blueberry extract, 1 to 3 parts of deep sea whole algae extract and 0.5 to 2 parts of xanthophyll. The traditional Chinese medicine composition can effectively treat xerophthalmia and asthenopia, and potential complication risks can be avoided.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition for treating dry eye and visual fatigue, and a preparation method and application thereof. Background Art

[0002] Dry eye (DE) is a chronic ocular surface disease that generally affects people's vision and quality of life. Dry eye is caused by abnormalities in the quality, quantity and dynamics of tears, resulting in tear film instability or imbalance of the ocular surface microenvironment, often accompanied by ocular surface inflammatory response, tissue damage and nerve abnormalities, which in turn cause a variety of eye discomfort symptoms and / or visual dysfunction. Dry eye can be divided into multiple types, including aqueous deficiency type, lipid abnormality type, mucin abnormality type, tear dynamics abnormality type and mixed type. Among them, lipid abnormality dry eye accounts for 60% to 90% of all dry eye cases, and Meibomian Gland Dysfunction (MGD) is the most common cause of lipid abnormality dry eye.

[0003] Visual fatigue is also a common disease in ophthalmology. Its symptoms include dry eyes, soreness, blurred vision, etc., which will directly affect people's daily work and life. Visual fatigue is mainly caused by staring at a close-up fluorescent screen for a long time, such as a computer screen, mobile phone, etc., which will lead to a decrease in the number of blinks, thereby reducing the secretion of tears. At the same time, the flickering screen will irritate the eyes. In addition, visual fatigue may also cause symptoms such as eye pain, dull pain in the eyeball, and unclear vision. In severe cases, symptoms of blood deficiency and liver fatigue caused by long-term overwork may appear.

[0004] The accelerated pace of life and increased work pressure in modern society have led to an increasing number of patients with dry eye and visual fatigue. The prevalence and high incidence of dry eye and visual fatigue have caused obvious discomfort to patients, such as dryness, burning sensation, foreign body sensation and pain, which seriously affect patients' visual function, daily activities and work.

[0005] Currently, dry eye is mainly treated by artificial tears to lubricate the ocular surface, anti-inflammatory treatment, and antibacterial treatment to reduce inflammation and improve symptoms, but this treatment method has potential complications. For example, the use of hormones and immunosuppressants may cause increased intraocular pressure and cataracts. Treatment of visual fatigue is mainly relieved by asking patients to rest, use their eyes less, and use eye drops, but the effect is limited.

[0006] Therefore, the existing treatment of dry eye has potential complications, and the treatment of visual fatigue has limited effect. Therefore, it is necessary to provide a product that can effectively treat dry eye and visual fatigue while avoiding potential complications. Summary of the invention

[0007] 1. Technical issues to be resolved

[0008] In order to solve the problems in the prior art of treating dry eye syndrome with potential complication risks and limited therapeutic effect on visual fatigue, the present invention provides a Chinese medicine composition for treating dry eye syndrome and visual fatigue, and a preparation method and application thereof.

[0009] (II) Technical solution

[0010] In order to achieve the above object, the main technical solutions adopted by the present invention include:

[0011] In a first aspect, the present invention provides a traditional Chinese medicine composition for treating dry eyes and visual fatigue, comprising, by weight, 45-55 parts of wild chrysanthemum, 35-45 parts of salvia miltiorrhiza, 8-12 parts of vitex rotundifolia, 8-12 parts of selfheal, 4-8 parts of buddlejac, 10-20 parts of cassia seed, 4-8 parts of coptis chinensis, 5-10 parts of qinpi, 5-8 parts of roxburghii, 10-15 parts of dittany, 0.1-0.5 parts of menthol, 0.1-0.3 parts of borneol, 3-5 parts of enzymatically hydrolyzed pearl powder, 2-5 parts of blueberry extract, 1-3 parts of deep-sea whole algae extract, and 0.5-2 parts of lutein.

[0012] The Chinese medicinal composition for treating dry eyes and visual fatigue as described above preferably comprises, by weight, 50-52 parts of wild chrysanthemum, 38-42 parts of salvia miltiorrhiza, 10-12 parts of vitex rotundifolia, 8-10 parts of selfheal, 5-7 parts of buddlejac, 12-16 parts of cassia seed, 5-7 parts of coptis root, 7-8 parts of qinpi, 7-8 parts of roxburghii, 12-14 parts of dittany, 0.2-0.3 parts of menthol, 0.2-0.3 parts of borneol, 4-5 parts of enzymatic pearl powder, 2-3 parts of blueberry extract, 2-3 parts of deep-sea whole algae extract, and 1-2 parts of lutein.

[0013] The Chinese medicine composition for treating dry eyes and visual fatigue as described above preferably comprises, by weight, 50 parts of wild chrysanthemum, 40 parts of salvia miltiorrhiza, 10 parts of vitex rotundifolia, 10 parts of selfheal, 6 parts of buddlejac, 14 parts of cassia seed, 6 parts of coptis chinensis, 7 parts of qinpi, 7 parts of roxburghii, 12 parts of dittany, 0.2 parts of menthol, 0.2 parts of borneol, 4 parts of enzymatic pearl powder, 3 parts of blueberry extract, 2 parts of deep-sea whole algae extract, and 1 part of lutein.

[0014] In a second aspect, the present invention provides a method for preparing the above-mentioned Chinese medicine composition for treating dry eye and visual fatigue, comprising the following steps:

[0015] S1: Washing and crushing wild chrysanthemum, salvia miltiorrhiza, vitex rotundifolia, herba prunellae, buddleja mongholicus, cassia seed, coptis root, qinpi, roxburghii and dittany to obtain a mixed medicinal material, adding water to the mixed medicinal material, and performing enzymatic hydrolysis on the mixed medicinal material;

[0016] S2: sterilizing the mixed medicinal materials after enzymatic hydrolysis, and then fermenting them to obtain a fermentation liquid;

[0017] S3: inactivating the fermentation liquid, and then performing ultrasonic low-temperature extraction to obtain an extract;

[0018] S4: extracting the extract to obtain an extract;

[0019] S5: The extract is concentrated under reduced pressure, and then menthol, borneol, enzymatically hydrolyzed pearl powder, blueberry extract, deep-sea whole algae extract and lutein are added and mixed to obtain a Chinese medicine composition.

[0020] In the method for preparing the Chinese medicine composition for treating dry eye and visual fatigue as described above, preferably, in step S1, water and enzymes are added to the mixed medicinal materials to perform enzymatic hydrolysis on the mixed medicinal materials; the enzyme is cellulase, pectinase or neutral protease.

[0021] The method for preparing the Chinese medicine composition for treating dry eye and visual fatigue as described above, preferably, in step S4, ethanol is added to the extract to perform an alcohol extraction to obtain an alcohol extract; then water is added to the extract after the alcohol extraction to perform two water extractions in sequence to obtain a first water extract and a second water extract, respectively;

[0022] The first water extract and the second water extract are mixed to obtain a mixed solution, ethanol is added to the mixed solution for alcohol precipitation and water extraction, and then the mixed solution is allowed to stand, and the precipitate is removed by filtering to obtain a supernatant;

[0023] The alcohol extract and the supernatant are mixed to obtain an extract.

[0024] The method for preparing the Chinese medicine composition for treating dry eye and visual fatigue as described above, preferably, in step S4, the volume concentration of ethanol used in the first alcohol extraction is 60-95%, the volume of ethanol is 3-5 times the volume of the extract, and the processing time of the first alcohol extraction is 1-3h;

[0025] In the two water extractions, the volume of water is 3-5 times the volume of the extract after the first alcohol extraction, and the processing time of the two water extractions is 1-2h respectively;

[0026] In the alcohol precipitation and water extraction, the volume concentration of ethanol added is 90-95%. After the addition of ethanol, the volume concentration of ethanol in the mixed solution is 60-70%, and the standing time is 20-28 hours.

[0027] In a third aspect, the present invention provides an application of the above-mentioned traditional Chinese medicine composition or the traditional Chinese medicine composition prepared by the above-mentioned preparation method in a non-woven eye patch. Specifically, glycerol, sodium benzoate and deionized water are added to the traditional Chinese medicine composition, and after uniform mixing, an eye patch liquid is obtained, and a non-woven fabric is immersed in the eye patch liquid to obtain a non-woven eye patch;

[0028] The eye patch solution comprises, by weight percentage, 0.1-0.3% of glycerol, 0.2-0.3% of sodium benzoate and 74-75% of deionized water, and the remainder is a traditional Chinese medicine composition.

[0029] In a fourth aspect, the present invention provides a use of the above-mentioned Chinese medicine composition or the Chinese medicine composition prepared by the above-mentioned preparation method in a fumigant. Specifically, sterile water is added to the Chinese medicine composition, and the mixture is heated to obtain a fumigant; wherein the mass ratio of the Chinese medicine composition to sterile water is 1:40-1:50.

[0030] In a fifth aspect, the present invention provides an application of the above-mentioned Chinese medicine composition or the Chinese medicine composition prepared by the above-mentioned preparation method in an eye ointment or atomized liquid. Specifically, the application of the Chinese medicine composition in the eye ointment comprises: adding medical vaseline, lanolin and liquid wax to the Chinese medicine composition, mixing them evenly, to obtain the eye ointment;

[0031] The application of the Chinese medicine composition in the atomized liquid comprises: adding sodium hyaluronate and sodium chloride to the Chinese medicine composition, mixing evenly, and filtering to obtain the atomized liquid.

[0032] (III) Beneficial effects

[0033] The present invention provides a Chinese medicine composition comprising wild chrysanthemum, salvia miltiorrhiza, vitex rotundifolia, selfheal, buddleja monnieri, cassia seed, coptis chinensis, qinpi, roxburghii, dittany, menthol, borneol, enzymatically hydrolyzed pearl powder, blueberry extract, deep-sea whole algae extract and lutein. The Chinese medicine composition can achieve significant therapeutic effects in treating dry eye and visual fatigue. According to clinical statistics, the total effective rate of the Chinese medicine composition in treating dry eye and visual fatigue can reach 90% or more, and there are few adverse reactions, which can avoid the risk of potential complications. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 The process flow chart of the preparation method of the Chinese medicine composition for relieving dry eyes and visual fatigue in the present invention;

[0035] Figure 2 This is a statistical chart of the average tear secretion of mice in the blank control group, negative control group, and experimental groups 1-8 after 14 days of administration;

[0036] Figure 3 This is a statistical graph of the total antioxidant capacity (T-AOC) in the lens of each group of mice after 14 days of administration;

[0037] Figure 4 This is a statistical graph of the detection of malondialdehyde (MDA) in the liver tissue of each group of mice after 14 days of administration. DETAILED DESCRIPTION

[0038] In order to better explain the present invention and facilitate understanding, the present invention is described in detail below in conjunction with specific implementation methods.

[0039] The invention provides a traditional Chinese medicine composition for treating dry eyes and visual fatigue. The traditional Chinese medicine composition comprises, by weight, 45-55 parts of wild chrysanthemum, 35-45 parts of salvia miltiorrhiza, 8-12 parts of vitex rotundifolia, 8-12 parts of selfheal, 4-8 parts of buddlejac, 10-20 parts of cassia seed, 4-8 parts of coptis root, 5-10 parts of qinpi, 5-8 parts of roxburghii, 10-15 parts of dittany, 0.1-0.5 parts of menthol, 0.1-0.3 parts of borneol, 3-5 parts of enzymatically hydrolyzed pearl powder, 2-5 parts of blueberry extract, 1-3 parts of deep-sea whole algae extract and 0.5-2 parts of lutein.

[0040] Preferably, the Chinese medicine composition for treating dry eyes and visual fatigue of the present invention comprises, by weight, 50-52 parts of wild chrysanthemum, 38-42 parts of salvia miltiorrhiza, 10-12 parts of vitex rotundifolia, 8-10 parts of selfheal, 5-7 parts of buddlejac, 12-16 parts of cassia seed, 5-7 parts of coptis root, 7-8 parts of qinpi, 7-8 parts of roxburghii, 12-14 parts of dittany, 0.2-0.3 parts of menthol, 0.2-0.3 parts of borneol, 4-5 parts of enzymatic pearl powder, 2-3 parts of blueberry extract, 2-3 parts of deep-sea whole algae extract, and 1-2 parts of lutein.

[0041] Further preferably, the Chinese medicine composition for treating dry eyes and visual fatigue of the present invention comprises, by weight, 50 parts of wild chrysanthemum, 40 parts of salvia miltiorrhiza, 10 parts of vitex rotundifolia, 10 parts of selfheal, 6 parts of buddlejac, 14 parts of cassia seed, 6 parts of coptis chinensis, 7 parts of qinpi, 7 parts of roxburghii, 12 parts of dittany, 0.2 parts of menthol, 0.2 parts of borneol, 4 parts of enzymatic hydrolyzed pearl powder, 3 parts of blueberry extract, 2 parts of deep-sea whole algae extract, and 1 part of lutein.

[0042] The prescription of the Chinese medicine composition of the present invention is explained as follows:

[0043] Dry eyes are a multifactorial chronic functional ocular surface disease, which is caused by decreased tear film stability and eye discomfort and ocular surface tissue lesions. Dry eyes are similar to the "white and astringent syndrome" in traditional Chinese medicine. Both are manifested as dry eyes without redness or swelling in the white of the eye, and even blurred vision. It was first seen in the Sui and Tang Dynasties in "Nagarjuna Bodhisattva Eye Theory·Eye Dryness Symptoms" in traditional Chinese medicine. The negative control group of "negative control group white and astringent syndrome" was first proposed in the negative control group of "Negative control group Scrutinizing Yaohan". Descriptions such as "eye dryness", "spirit exhausted" or "eye dryness and difficulty opening" can all be attributed to the category of "white and astringent syndrome". When the name of the disease was standardized after the negative control group, it was uniformly named "white and astringent syndrome" and has been used to this day. With reference to the records in Huangdi Neijing and Shenshen Yaohan and based on the current clinical reality, it can be specifically summarized into 6 syndromes: insufficient lung yin, dryness and heat damaging body fluid; long-term evil heat, dryness and heat damaging the lungs; stagnation of liver meridian, insufficient liver yin; deficiency of liver and kidney, loss of yin essence; deficiency of spleen qi, inability of spleen to transform body fluid; damp heat in the spleen and stomach, and spleen deficiency and dampness.

[0044] Visual fatigue is a syndrome caused by various causes that make the human eye look at objects with a load that exceeds the visual function, resulting in visual impairment, eye discomfort or systemic symptoms after using the eyes, making it impossible to perform normal visual tasks. Symptoms include intolerance to long-term viewing, dry eyes, itchy eyes, eye swelling and pain, and systemic discomfort, which seriously affect the patient's study, work and life. Its etiology and pathogenesis are considered by TCM diagnosis and treatment to be "liver fatigue". Its etiology and pathogenesis can be summarized as follows: (1) liver and kidney deficiency, loss of essence and blood, and inability to nourish the body; (2) spleen deficiency and weak qi, failure of the clear yang to rise, and loss of nourishment to the eyes; (3) fatigue and exhaustion of vision, deficiency of heart blood, and loss of nourishment to the eyes; (4) excessive emotional injury, liver depression and qi stagnation, or excessive use of the eyes, qi and blood stagnation, resulting in stagnation of the meridians, insufficient qi and blood, and inability to nourish the eyes.

[0045] In summary, it can be seen that the etiology and pathogenesis of dry eyes and visual fatigue are closely related. The Chinese medicine composition of the present invention comprehensively dialectically treats dry eyes and visual fatigue, and through the reasonable combination of monarch, minister, assistant and envoy, takes clearing away heat and detoxification, promoting blood circulation and removing blood stasis, regulating qi and relieving depression, dispersing wind and clearing heat, drying dampness and improving eyesight, nourishing the liver and kidneys, and supplementing nutrition as the treatment principles, promotes tear secretion, stabilizes tear film, and relieves the symptoms of dry eyes and visual fatigue.

[0046] Among them, wild chrysanthemum and salvia miltiorrhiza are the main medicines: wild chrysanthemum is bitter, pungent and slightly cold in nature, enters the liver and heart meridians, has the functions of dispersing wind and clearing heat, calming the liver and improving eyesight, clearing away heat and detoxifying, can remove heat and toxins from the eyes, and relieve symptoms such as redness, swelling and pain caused by dry eyes and visual fatigue; salvia miltiorrhiza is bitter, slightly cold in nature, enters the heart and liver meridians, and has the functions of dredging meridians and activating collaterals, removing blood stasis and relieving pain, eliminating restlessness and calming the mind, and on the other hand, it can clear away heat and detoxify together with wild chrysanthemum, and on the other hand, it can promote blood circulation and dredge collaterals, improve eye congestion in patients with dry eyes and visual fatigue, and relieve the eye and systemic symptoms of dry eyes and visual fatigue.

[0047] Vitex, Herba Prunellae, Buddleja, and Semen Cassiae are the assistant drugs: Vitex is bitter and slightly cold in nature, and enters the bladder, liver, and stomach meridians. It can dissipate wind-heat and clear the head, and assist the main drug to enhance the effect of dispersing wind and relieving pain, and assist in the treatment of headaches, toothaches, dizziness, and other accompanying symptoms; Herba Prunellae is bitter and cold in nature, and enters the liver and gallbladder meridians. It can clear liver fire, dissipate depression, and nourish blood and soothe the liver. When used with wild chrysanthemum, its soothing effect is enhanced, and when used with Salvia miltiorrhiza, its soothing effect is enhanced. Its depression-relieving effect can make patients with dry eyes and visual fatigue have moist eyes and calm their minds; Buddleja buddhist flower is sweet in nature, slightly cold, and enters the liver meridian. It synergizes with Prunella vulgaris and Cassia seed to assist the main drug in clearing away and tonifying, and is mainly used to expel evil and strengthen the body, so as to clear liver heat and nourish liver blood, and the liver obtains blood and the eyes are clear; Cassia seed is sweet, bitter, salty, slightly cold, and enters the liver, kidney, and large intestine meridians, which can benefit kidney yin and improve eyesight, and assist the main drug in regulating the liver and kidney, and enhance the main drug's functions of clearing heat and detoxifying, calming the liver and improving eyesight, and dispersing wind and relieving depression.

[0048] Coptis chinensis, Fragrant osmanthus, Noxious thread orchid and Dictamni are adjuvants: Coptis chinensis is bitter and cold in nature, and enters the heart, spleen, stomach, liver, gallbladder, and large intestine meridians; Fragrant osmanthus is bitter and cold in nature, and enters the liver, gallbladder, and large intestine meridians; Dictamni is cold in nature and bitter in taste, and enters the liver and spleen meridians. The combination of the three can clear away heat and dampness, dispel wind and detoxify, remove dampness and heat in the middle burner, assist the monarch and ministerial drugs in regulating the triple burner, and make the internal organs' qi and body fluids flow peacefully. Noxious thread orchid is used as an adjuvant, which is cool in nature and sweet in taste, and enters the liver, lung, kidney, and bladder meridians, clears heat and cools blood, removes dampness and detoxifies, strengthens the effects of clearing heat and detoxifying, calming the liver and improving eyesight, assists the monarch and ministerial drugs in regulating the qi of the five wheels, and is combined with Salvia miltiorrhiza to strengthen its depression-relieving effect.

[0049] Menthol and borneol are the adjuvant drugs: Menthol is pungent and cool in nature, and enters the lung and liver meridians. It has the effects of dispersing wind-heat, relieving liver depression, clearing the head and eyes, and clearing rashes. Borneol is pungent and slightly cold in nature. It has the effects of opening all orifices, dispersing depression, removing cataracts and improving eyesight, and relieving swelling and pain. Mint and borneol are compatible, harmonize all drugs, synergistically lead all drugs upward, open all orifices, make all qi and body fluids upward unobstructed, enhance the efficacy, and treat dry eyes and visual fatigue.

[0050] Enzymatic pearl powder, blueberry extract, and deep-sea whole algae extract are other ingredients. Enzymatic pearl powder has the effects of calming the nerves, improving eyesight, removing cataracts, detoxifying and promoting skin regeneration, moisturizing and removing freckles; blueberry extract is rich in antioxidant ingredients such as anthocyanins, vitamin C and vitamin E, and has a strong antioxidant capacity, which can remove free radicals, reduce oxidative stress, and protect cells from damage; deep-sea whole algae extract has the effects of nourishing yin and moisturizing dryness, clearing away heat and detoxifying, and anti-oxidation. Adding the above three ingredients to the traditional Chinese medicine external patch can further supplement nutrition for the eyes, protect the skin around the eyes, remove free radicals and resist oxidation, and improve dry eyes and visual fatigue, dry eyes, astringency, redness and pain, and other uncomfortable symptoms.

[0051] The detailed pharmacology of the above components are as follows:

[0052] Wild chrysanthemum: bitter, pungent, slightly cold, enters the liver and heart meridians. It is good at dispersing wind and clearing heat, calming the liver and improving eyesight, clearing heat and detoxifying. It is mainly used to treat headaches, dizziness, red eyes, chest irritability, furuncles, swelling and other symptoms. Modern pharmacological studies have shown that wild chrysanthemum can effectively control inflammation and has an anti-inflammatory effect on 2,2-biphenyl-1-picrylhydrazyl free radicals (DPPH) and superoxide anion free radicals (O 2- ) and hydroxyl free radicals (OH), and its scavenging ability increases with increasing dose, and its effect is better than that of vitamin C, rutin and BHT.

[0053] Danshen: bitter in nature and slightly cold, it enters the heart and liver meridians. It has the effects of promoting blood circulation and removing blood stasis, regulating menstruation and relieving pain, relieving restlessness and calming the nerves, cooling blood and eliminating carbuncle, and is a commonly used blood circulation and blood stasis medicine. Modern pharmacological studies have shown that Danshen can be used to treat cardiovascular and cerebrovascular diseases, such as coronary heart disease and angina pectoris, because its active ingredients can resist arrhythmia, dilate coronary arteries, increase coronary blood flow, improve microcirculation, protect the myocardium, and improve the heart's tolerance to ischemia and hypoxia. At the same time, Danshen can also reduce blood viscosity, resist platelet aggregation, and prevent thrombosis.

[0054] Vitex rotundifolia: bitter and slightly cold in nature, it enters the bladder, liver and stomach meridians. It has the effects of dispersing wind-heat and clearing the head, and is used to treat wind-heat cold headache, swollen and painful teeth and eyes, red eyes and tears, blurred vision, dizziness and other symptoms. Modern pharmacological studies have shown that Vitex rotundifolia can block acute inflammatory responses by downregulating NF-κB signal transduction, increasing anti-inflammatory cytokines, reducing polymorphonuclear granulocyte infiltration, and inhibiting the production of proinflammatory cytokines and chemokines. Flavonoids and phenolic acid components are the main components of Vitex rotundifolia's antioxidant activity. The ethanol extract of Vitex rotundifolia has a significant scavenging ability against hydroxyl, hydrogen peroxide and nitric oxide free radicals.

[0055] Prunella Vulgaris: bitter and pungent in nature, cold, and enters the liver and gallbladder meridians. It has the effects of clearing liver fire and dispersing depression, nourishing blood and calming the liver, and is suitable for symptoms such as liver fire, hyperactivity of yang, and phlegm and stagnation. Modern pharmacological studies have shown that Prunella Vulgaris has anti-inflammatory and immune-regulating effects.

[0056] Buddleja buddha: sweet in nature and slightly cold, it belongs to the liver meridian. It has the effects of clearing and replenishing, mainly dispelling evil and strengthening the body, clearing liver heat and nourishing liver blood. It is an important medicine for treating eye diseases and is suitable for those with liver fire inflammation or liver blood deficiency with heat. Modern pharmacological studies have shown that buddha buddha has antioxidant, anti-inflammatory, anti-tumor, anti-cardiovascular, antibacterial, hypoglycemic, neuroprotective, and immunomodulatory effects.

[0057] Cassia seed: sweet, bitter and salty in nature, slightly cold, and enters the liver, kidney and large intestine meridians. It has the effects of clearing liver fire, benefiting kidney yin and improving eyesight, and is suitable for symptoms such as red, swollen and painful eyes and blurred vision. Cassia seed can also clear heat, moisten the intestines and relieve constipation, and is suitable for constipation caused by heat and dryness of the colon. Clinical experiments have shown that Cassia seed can clear the liver and improve eyesight, prevent blurred vision, lower blood pressure, lower blood lipids, and reduce cholesterol. It has a good effect on preventing and treating coronary heart disease and hypertension; it is rich in vitamin A and zinc, and can prevent and treat night blindness and zinc deficiency in children.

[0058] Coptis chinensis: bitter and cold in nature, it enters the heart, spleen, stomach, liver, gallbladder, and large intestine meridians. Coptis chinensis has the effects of clearing heat and purging fire, drying dampness, and removing dampness and toxicity. It is an important medicine for treating damp-heat and fire toxicity and is widely used in damp-heat and fire toxicity syndromes. Compared with Scutellaria baicalensis, Coptis chinensis has a stronger ability to clear heat and dry dampness, and its effect is biased towards the heart and the middle-jiao spleen and stomach. It is best at clearing the fire of the heart and stomach and removing dampness and heat in the middle-jiao. Modern pharmacological studies have shown that berberine in Coptis chinensis alkaloids is the main active ingredient that exerts its anti-inflammatory effect.

[0059] Cortex Fraxini: bitter and cold in nature, it enters the liver, gallbladder and large intestine meridians. It has the effects of clearing away heat and dampness, astringing and stopping dysentery, stopping leukorrhea and improving eyesight. It is used for symptoms such as damp-heat diarrhea, leukorrhea, red and swollen eyes, and eye film. Cortex Fraxini mainly contains coumarin components such as fraxinic acid, fraxinic acid ethyl, fraxinic acid, fraxinin, etc., as well as phenols, saponins, tannins, etc. Modern pharmacological studies have shown that Cortex Fraxini has the effect of improving inflammatory diseases, and its mechanism of action is mostly related to slowing down tissue lesions and lowering the level of inflammatory factors. Fraxini polyphenols have a certain inhibitory effect on DPPH and ABTS free radicals, and the inhibitory effect on ABTS free radicals is stronger than that on DPPH free radicals. The antioxidant effect of Fraxini polyphenols increases with the increase of its mass concentration, and within a certain concentration range, the antioxidant capacity of Fraxini polyphenols is stronger than that of vitamins.

[0060] Golden thread orchid: cool in nature, sweet in taste, and enters the liver, lung, kidney, and bladder meridians. It has the effects of clearing away heat and detoxifying, moistening the lungs and relieving cough, calming the liver and improving eyesight. It is used to treat symptoms such as lung heat cough, sore throat, red and swollen eyes, and liver fire inflammation. Modern pharmacological studies have shown that Golden thread orchid has liver protection, hypoglycemic, anti-inflammatory, and antibacterial effects.

[0061] Dictamni: cold in nature, bitter in taste, enters the liver and spleen meridians. It has the effects of clearing away heat and dampness, dispelling wind and detoxifying, and is used to treat symptoms such as damp-heat jaundice, eczema itching, rheumatic pain, etc. Modern pharmacological studies have shown that Dictamni has anti-inflammatory and anti-allergic effects, protects the liver and nerves, protects the cardiovascular system, improves immunity, and anti-aging effects.

[0062] Menthol: It is pungent and cool in nature and enters the lung and liver meridians. It has the effects of dispersing wind-heat, clearing the head and throat, and clearing rashes. It can also relieve liver depression and eliminate filth. Menthol has a strong sweating ability and is especially good at clearing the head. It is suitable for treating symptoms of wind-heat attacking the surface or attacking the upper part, and can also be used to treat liver depression and heat. Modern pharmacological studies have shown that menthol has anti-inflammatory effects and can inhibit a variety of chemical substances in inflammatory reactions, such as prostaglandins, leukotrienes, etc., inhibit inflammatory reactions, and relieve symptoms such as pain and swelling.

[0063] Borneol: bitter and slightly cold in nature. It has the effects of opening all the orifices, dispersing stagnation, removing cataracts and improving eyesight, and relieving swelling and pain. Modern pharmacological studies have shown that borneol has anti-inflammatory and antioxidant effects. It can play an antioxidant role by scavenging oxygen free radicals and inhibiting lipid peroxidation. It can also enhance the body's antioxidant capacity by activating antioxidant signaling pathways, such as the Nrf2 / ARE pathway.

[0064] Enzyme-hydrolyzed pearl powder: It has the effects of calming the nerves, improving eyesight, removing cataracts, detoxifying and promoting tissue regeneration, moisturizing and removing freckles. Modern pharmacological studies have shown that enzyme-hydrolyzed pearl powder has anti-inflammatory effects, which can reduce the production and release of inflammatory mediators, thereby reducing inflammatory manifestations such as redness, swelling, heat and pain; it has an antioxidant effect, which can improve the body's antioxidant capacity, fight against the damage caused by free radicals to cells, and play a role in delaying aging; it has the effect of improving immunity, which can stimulate the body to produce immune responses and enhance resistance.

[0065] Blueberry extract: Rich in antioxidant ingredients such as anthocyanins, vitamin C and vitamin E, it has strong antioxidant capacity, can scavenge free radicals, reduce oxidative stress and protect cells from damage.

[0066] Deep sea algae extract: It has the effects of nourishing yin and moistening dryness, clearing away heat and detoxifying, and anti-oxidation. Modern pharmacological studies have shown that its antioxidant effect is mainly through its active ingredients to remove free radicals, enhance the antioxidant capacity of cells, and protect cells from oxidative stress damage.

[0067] Lutein: It can protect retinal cells and prevent macular degeneration. It also has antioxidant and anti-inflammatory effects, nourishes eye tissues and improves eye immunity.

[0068] In summary, it can be seen that the Chinese medicine composition of the present invention also has sufficient theoretical support in modern pharmacological research, and the Chinese medicine composition of the present invention can have a good therapeutic and alleviating effect on dry eye and visual fatigue.

[0069] Reference Figure 1 The present invention also provides a method for preparing the above-mentioned Chinese medicine composition for treating dry eye and visual fatigue, comprising the following steps:

[0070] S1: Wash and crush wild chrysanthemum, salvia miltiorrhiza, vitex rotundifolia, herba prunellae, buddleja buddleja, cassia seed, coptis root, qinpi, roxburghii and dittany to obtain a mixed medicinal material, add water to the mixed medicinal material, and perform enzymatic hydrolysis on the mixed medicinal material.

[0071] S2: sterilizing the mixed medicinal materials after enzymatic hydrolysis, and then fermenting them to obtain fermentation liquid.

[0072] S3: inactivating the fermentation liquid, and then performing ultrasonic low-temperature extraction to obtain an extract;

[0073] S4: extracting the extract to obtain an extract.

[0074] S5: The extract is concentrated under reduced pressure, and then menthol, borneol, enzymatically hydrolyzed pearl powder, blueberry extract, deep-sea whole algae extract and lutein are added and mixed to obtain a Chinese medicine composition.

[0075] Preferably, in step S1, water and enzymes are added to the mixed medicinal materials to perform enzymatic hydrolysis on the mixed medicinal materials. The enzyme in this step can be any one of cellulase, pectinase or neutral protease, or a combination of two or more thereof.

[0076] Cellulose is the main component of plant cell walls. Cellulase can catalyze the hydrolysis of cellulose, breaking it down into smaller molecules, opening the plant cell walls, making it easier to release the effective ingredients in the cells and improving subsequent extraction efficiency.

[0077] Pectin is also an important component of plant cell walls. Pectinase can catalyze the hydrolysis of pectin, breaking it down into smaller molecules, further opening the plant cell walls, making it easier to release the effective ingredients in the cells and improving subsequent extraction efficiency.

[0078] Neutral proteases can catalyze the decomposition of proteins in plant cells, release the active ingredients bound to the proteins, and improve the subsequent extraction efficiency.

[0079] By using cellulase, pectinase and neutral protease or a combination thereof to enzymatically hydrolyze Chinese medicinal materials, the extraction efficiency of the active ingredients in the medicinal materials can be effectively improved, and the energy consumption and time in the extraction process can be reduced.

[0080] Preferably, in step S2, gamma ray sterilization or high temperature steam sterilization, preferably gamma ray sterilization, can be used to ensure that the liquid medicine is sterile and avoid affecting the subsequent fermentation process. After sterilization, the nutrients can be prepared, and then suitable strains can be selected, including but not limited to lactic acid bacteria, yeasts, or mixtures thereof, for inoculation. The inoculated Chinese medicine mixture is transferred to a fermentation tank, and the temperature, humidity, pH value and other conditions of the fermentation tank are controlled. Stirring can be performed by stirring or shaking, so that the strains are fully in contact with the medicinal materials to promote the fermentation process.

[0081] Preferably, in step S3, the fermentation liquid is inactivated to kill the microorganisms in the fermentation liquid to prevent further fermentation, and then filtered to remove solid residues. The fermentation liquid is then subjected to ultrasonic low-temperature extraction to obtain an extract. Specifically, a container containing the fermentation liquid is placed in an ultrasonic generator, and a high-frequency vibration wave is generated by the ultrasonic generator. The high-frequency vibration wave forms tiny bubbles in the fermentation liquid. These bubbles will collapse rapidly after growing to a certain size, producing a cavitation effect. The cavitation effect helps to break the cell wall structure, so that the effective ingredients in the cell are more fully released. During the entire ultrasonic treatment process, the temperature of the fermentation liquid needs to be maintained at 30-40°C.

[0082] Preferably, in step S4, ethanol is added to the extract to perform an alcohol extraction to obtain an alcohol extract; then water is added to the extract after the alcohol extraction, and water extraction is performed twice in sequence to obtain a first water extract and a second water extract, respectively. The first water extract and the second water extract are mixed to obtain a mixed solution, ethanol is added to the mixed solution to perform alcohol precipitation water extraction, and then the mixture is allowed to stand, and the precipitate is filtered to remove the supernatant to obtain a supernatant. The alcohol extract is mixed with the supernatant to obtain an extract.

[0083] Further preferably, in step S4, the volume concentration of ethanol used in the first alcohol extraction is 60-95%, the volume of ethanol is 3-5 times the volume of the extract, and the processing time of the first alcohol extraction is 1-3h. In the two water extractions, the volume of water is 3-5 times the volume of the extract after the first alcohol extraction, and the processing time of the two water extractions is 1-2h respectively. In the alcohol precipitation water extraction, the volume concentration of ethanol added is 90-95%, the volume concentration of ethanol in the mixed solution after adding ethanol is 60-70%, and the standing time is 20-28h.

[0084] The one alcohol extraction and two water extractions may be pressurized extractions, the pressure may be 0.2-0.3 MPa, and the extraction temperature may be 50-60°C.

[0085] In the present invention, the above-mentioned Chinese medicine composition or the Chinese medicine composition prepared by the above-mentioned preparation method can be used for non-woven eye patches. The preparation method of the non-woven eye patch is: add glycerol, sodium benzoate and deionized water to the Chinese medicine composition, mix them evenly to obtain an eye patch liquid, and immerse the non-woven fabric in the eye patch liquid to obtain a non-woven eye patch. In the eye patch liquid, according to the weight percentage, it includes 0.1-0.3% glycerol, 0.2-0.3% sodium benzoate and 74-75% deionized water, and the balance is the Chinese medicine composition.

[0086] In the present invention, the above-mentioned Chinese medicine composition or the Chinese medicine composition prepared by the above-mentioned preparation method can be used as a fumigant. The preparation method of the fumigant is: adding sterile water to the Chinese medicine composition, heating, and obtaining a fumigant with a temperature of 40-50° C. wherein the mass ratio of the Chinese medicine composition to the sterile water is 1:40-1:50.

[0087] In the present invention, the above-mentioned Chinese medicine composition or the Chinese medicine composition prepared by the above-mentioned preparation method can be used in eye ointment. The preparation method of the eye ointment is: add medical vaseline, lanolin and liquid wax to the Chinese medicine composition, mix them evenly, and obtain the eye ointment. In the present invention, the above-mentioned Chinese medicine composition or the Chinese medicine composition prepared by the above-mentioned preparation method can also be used in atomized liquid. The preparation method of the atomized liquid is: add sodium hyaluronate and sodium chloride to the Chinese medicine composition, mix them evenly, and obtain the atomized liquid by filtering.

[0088] In order to further clarify the scheme of the present invention and its technical advancement, the following is a description in conjunction with specific embodiments and technical effects.

[0089] Example 1

[0090] The present embodiment provides a traditional Chinese medicine composition, which includes, by weight, 50 parts of wild chrysanthemum, 40 parts of salvia miltiorrhiza, 10 parts of vitex rotundifolia, 10 parts of selfheal, 6 parts of buddlejac, 14 parts of cassia seed, 6 parts of coptis chinensis, 7 parts of qinpi, 7 parts of roxburghii, 12 parts of dittany, 0.2 parts of menthol, 0.2 parts of borneol, 4 parts of enzymatically hydrolyzed pearl powder, 3 parts of blueberry extract, 2 parts of deep-sea whole algae extract, and 1 part of lutein.

[0091] Glycerin, sodium benzoate and deionized water were added to the traditional Chinese medicine composition of this embodiment, and the mixture was uniformly mixed to obtain an eye patch solution, and the non-woven fabric was immersed in the eye patch solution to obtain a non-woven fabric eye patch. The eye patch solution includes 0.166% glycerin, 0.265% sodium benzoate and 74.6% deionized water by weight, and the remainder is the traditional Chinese medicine composition.

[0092] Example 2

[0093] The present embodiment provides a traditional Chinese medicine composition, which includes, by weight, 52 parts of wild chrysanthemum, 38 parts of salvia miltiorrhiza, 12 parts of vitex rotundifolia, 8 parts of selfheal, 7 parts of buddlejac, 12 parts of cassia seed, 7 parts of coptis chinensis, 8 parts of qinpi, 8 parts of roxburghii, 14 parts of dittany, 0.3 parts of menthol, 0.3 parts of borneol, 5 parts of enzymatically hydrolyzed pearl powder, 2 parts of blueberry extract, 3 parts of deep-sea whole algae extract, and 2 parts of lutein.

[0094] Sterile water was added to the Chinese medicine composition of this example and heated to obtain a fumigant with a temperature of 45° C. The mass ratio of the Chinese medicine composition to sterile water was 1:45.

[0095] Example 3

[0096] The present embodiment provides a traditional Chinese medicine composition, which includes, by weight, 45 parts of wild chrysanthemum, 42 parts of salvia miltiorrhiza, 8 parts of vitex rotundifolia, 12 parts of selfheal, 5 parts of buddlejac, 16 parts of cassia seed, 5 parts of coptis chinensis, 5 parts of qinpi, 5 parts of roxburghii, 10 parts of dittany, 0.1 part of menthol, 0.1 part of borneol, 3 parts of enzymatically hydrolyzed pearl powder, 5 parts of blueberry extract, 1 part of deep-sea whole algae extract, and 0.5 part of lutein.

[0097] Add medical vaseline, lanolin and liquid wax to the traditional Chinese medicine composition prepared in this example, mix well, and obtain an eye ointment. The eye ointment includes 20% of the traditional Chinese medicine composition, 60% of the medical vaseline, 10% of the lanolin and 10% of the liquid wax by mass percentage.

[0098] Example 4

[0099] The present embodiment provides a traditional Chinese medicine composition, which includes, by weight, 55 parts of wild chrysanthemum, 35 parts of salvia miltiorrhiza, 12 parts of vitex rotundifolia, 10 parts of selfheal, 4 parts of buddlejac, 10 parts of cassia seed, 4 parts of coptis chinensis, 10 parts of qinpi, 8 parts of roxburghii, 15 parts of dittany, 0.5 parts of menthol, 0.2 parts of borneol, 4 parts of enzymatically hydrolyzed pearl powder, 3 parts of blueberry extract, 2 parts of deep-sea whole algae extract, and 1 part of lutein.

[0100] Glycerin, sodium benzoate and deionized water are added to the Chinese medicine composition of this embodiment, and the mixture is uniformly mixed to obtain an eye patch solution, and the non-woven fabric is immersed in the eye patch solution to obtain a non-woven fabric eye patch. The eye patch solution includes 0.2% glycerin, 0.3% sodium benzoate and 75% deionized water by weight, and the remainder is the Chinese medicine composition.

[0101] Example 5

[0102] The present embodiment provides a traditional Chinese medicine composition, which includes, by weight, 50 parts of wild chrysanthemum, 45 parts of salvia miltiorrhiza, 10 parts of vitex rotundifolia, 10 parts of selfheal, 8 parts of buddlejac, 20 parts of cassia seed, 8 parts of coptis chinensis, 7 parts of qinpi, 7 parts of roxburghii, 12 parts of dittany, 0.2 parts of menthol, 0.2 parts of borneol, 4 parts of enzymatically hydrolyzed pearl powder, 3 parts of blueberry extract, 2 parts of deep-sea whole algae extract, and 1 part of lutein.

[0103] Glycerin, sodium benzoate and deionized water are added to the Chinese medicine composition of this embodiment, and the mixture is uniformly mixed to obtain an eye patch solution, and the non-woven fabric is immersed in the eye patch solution to obtain a non-woven fabric eye patch. The eye patch solution includes 0.3% glycerin, 0.2% sodium benzoate and 74% deionized water by weight, and the remainder is the Chinese medicine composition.

[0104] Comparative Example 1

[0105] This comparative example provides a traditional Chinese medicine composition, which is different from Example 1 in that the amount of Nox villosum in the traditional Chinese medicine composition is 0.

[0106] Comparative Example 2

[0107] This comparative example provides a traditional Chinese medicine composition, which is different from Example 1 in that the amount of Dictamni cortex in the traditional Chinese medicine composition is 0.

[0108] Comparative Example 3

[0109] This comparative example provides a traditional Chinese medicine composition, which is different from Example 1 in that the proportions of Nox villosum roxburghii and Dictamni cortex in the traditional Chinese medicine composition are both 0.

[0110] Test Example 1:

[0111] The effects of the Chinese medicine compositions prepared in Examples 1-5 and Comparative Examples 1-3 on dry eye in mice were investigated:

[0112] Healthy 7-week-old BALB / c mice were selected, half male and half female. The health standard was: mice with abnormal corneal fluorescence staining and large difference in tear secretion between the two eyes were eliminated through corneal fluorescence staining scores and tear secretion volume measurement.

[0113] The selected mice were divided into a blank control group, a negative control group, experimental group 1, experimental group 2, experimental group 3, experimental group 4, experimental group 5, experimental group 6, experimental group 7 and experimental group 8. There were 4 mice in each group, and the number of eyes in each group was 8. The number of eyes was averaged when calculating the tear secretion amount below. Among them, the mice in the blank control group were not treated in any way except normal feeding. The eyes of the negative control group and experimental groups 1-8 were dripped with benzalkonium chloride solution at a concentration of 1.5 mg / ml, 3 times a day, for 14 consecutive days.

[0114] After the dry eye model was established, normal saline was dripped into both eyes of mice in the blank control group and the negative control group every day, and equal volumes of eye drops prepared from the Chinese medicine compositions prepared in Examples 1-5 and Comparative Examples 1-3 were dripped into both eyes of mice in Experimental Groups 1 to 8, respectively. The frequency of administration was: 5 times a day for 14 consecutive days.

[0115] The phenol red cotton thread experiment was used to measure the average tear secretion of each group of mice after successful modeling (day 0) and the tear secretion after 14 days of administration. Specifically, the phenol red cotton thread was clamped with ophthalmic forceps and then placed on the outer canthus of the mouse's eye. It was taken out after 60 seconds and the wet length of the phenol red cotton thread (mm) was measured. The specific results are shown in Table 1 and Figure 2 Among them, the tear secretion volume in Table 1 is the average tear secretion volume after modeling, the unit is mm, specifically the mean ± standard deviation; Figure 2 Groups I-X correspond to the average tear secretion of mice in the blank control group, negative control group, experimental group 1, experimental group 2, experimental group 3, experimental group 4, experimental group 5, experimental group 6, experimental group 7 and experimental group 8 after administration for 14 days, respectively, in mm.

[0116] Table 1 Statistics of the average tear secretion of mice in the blank control group, negative control group and experimental groups 1-8

[0117]

[0118]

[0119] Figure 2-Figure 4 In the table, the same letters (abcd) indicate that there is no statistical difference between the corresponding groups (P>0.05), while different letters indicate that there is a statistical difference between the corresponding groups (P<0.05).

[0120] Table 1 and Figure 2 It can be seen that the average tear secretion of mice in the blank control group, which did not undergo modeling and drug administration, remained normal throughout. After modeling, the average tear secretion of mice in the negative control group and experimental groups 1 to 8 was significantly reduced, indicating that the modeling was successful. The mice in the negative control group were only instilled with normal saline, and the average tear secretion did not improve. After 14 days of drug administration, the average tear secretion of mice in experimental groups 1 to 5 was greater than 5mm / 1min, returning to normal. Although the average tear secretion of mice in experimental groups 6-8 was improved, it has not yet returned to normal levels. The average tear secretion of mice in experimental groups 6 and 7 increased more than that in experimental group 8.

[0121] Test Example 2:

[0122] The effects of the Chinese medicine compositions prepared in Examples 1-5 and Comparative Examples 1-3 on visual fatigue in mice were investigated:

[0123] 1. Animal selection:

[0124] Healthy BALB / c mice aged 7 weeks were selected, weighing (18±1) g, and kept at 23±2°C and relative humidity of 55-75%. The mice were placed in white plastic cages, and given normal light from 7 am to 7 pm, and no light at other times, and were provided with sufficient food and water.

[0125] 2. Modeling:

[0126] The selected mice were evenly divided into a blank control group, a negative control group, experimental group 1, experimental group 2, experimental group 3, experimental group 4, experimental group 5, experimental group 6, experimental group 7 and experimental group 8. Among them, the mice in the blank control group were not treated in any way except for normal feeding.

[0127] The mice in the negative control group and experimental groups 1-8 were treated as follows:

[0128] The mice were exposed to strong light in a closed dark room without interference from natural light. Each mouse was placed individually in a small square made of acrylic transparent board, and eye drops were applied to the mouse eyeballs before illumination to avoid dehydration of the eyeball surface due to long-term illumination. The illuminator was hung 50 cm from the top of the mouse, and the irradiation was carried out vertically. A thermometer was hung at the same height as the mouse to monitor the temperature in real time to exclude the possibility of photothermal damage to the mouse eyeball caused by temperature increase. Specifically, the mice in the negative control group and the experimental group were irradiated for 4 hours a day and continuously for 7 days at a light intensity of (3000±500) Lux. They ate and drank water normally during the illumination period, and knocked on the cage during the irradiation process to prevent the mice from drowsiness, laziness or lying down, and to keep the mice in a continuous eye state.

[0129] After the asthenopia model was established, physiological saline was dripped into both eyes of mice in the blank control group and the negative control group every day, and equal volumes of eye drops prepared from the Chinese medicine composition prepared in Examples 1-5 and Comparative Examples 1-3 were dripped into both eyes of mice in Experimental Groups 1 to 8, respectively. The frequency of administration was: 3 times a day for 4 consecutive weeks.

[0130] At the end of the experiment, the mice were killed, their eyeballs were removed, and the lenses were peeled off from the eyeballs. 9 volumes of normal saline were added, and the mixture was pulped and centrifuged at 4°C. The supernatant was taken and the T-AOC in the lenses of the mice in each group was determined using the total antioxidant capacity (T-AOC) detection kit. The results are shown in Figure 3 .

[0131] T-AOC (Total Antioxidant Capacity) refers to the total antioxidant capacity of all antioxidant substances and antioxidant enzymes in an organism, reflecting the overall function of the antioxidant defense system in the body, as well as the comprehensive ability to scavenge free radicals and resist oxidative stress. When the T-AOC content decreases, the body's ability to scavenge free radicals decreases, oxidative stress is more likely to occur, and cells and tissues are more susceptible to damage by free radicals, which may lead to cell apoptosis, inflammatory response and the occurrence of chronic diseases. Figure 3 It can be seen that the T-AOC content of the blank control group and the negative control group was 6.18±0.59nmol / mg and 1.40±0.16nmol / mg, respectively, indicating that excessive light exposure can cause a significant decrease in the T-AOC content in the liver tissue of mice. Compared with the model group, the experimental groups 1 to 5 were able to significantly increase the T-AOC content, and although the experimental groups 6 to 8 were able to increase the T-AOC content to a certain extent, the degree of increase was not obvious.

[0132] In addition, after killing the mice, the liver tissues removed from the mice were immediately placed in a refrigerator for storage for the determination of the biochemical index MDA to be tested. When preparing liver tissue homogenate, the cryopreserved liver tissues were taken out, and ice saline was added at a solid-liquid ratio of 1:9 (g / mL). The liver tissues in the centrifuge tube were ground into uniform tissues using a grinding rod under low temperature conditions, and then centrifuged in a low-temperature high-speed centrifuge at 4°C and 3000r / min for 10 minutes. The supernatant was taken and the MDA in the liver tissues of each group of mice was determined using the malondialdehyde (MDA) kit. The results are shown in Figure 4 .

[0133] Excessive light exposure can cause a large number of free radicals to be produced in mice, including malondialdehyde free radicals, which can attack polyunsaturated fatty acids in biomembranes. Excessive production of these substances can also cause an imbalance in the body's antioxidant balance system and damage the synthesis, cleavage and transcription of cell DNA in the body, thereby destroying the biomembrane structure and causing irreversible damage to lipids in the body. Figure 4 It can be seen that the MDA content of the blank control group and the negative control group was 1.29±0.15nmol / mg and 3.75±0.41nmol / mg, respectively, indicating that excessive light exposure can significantly increase the MDA content in the liver tissue of mice. Compared with the model group, the MDA content of experimental groups 1 to 5 was significantly reduced, and although the MDA content of experimental groups 6 to 8 was reduced, the degree of reduction was not obvious.

[0134] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese medicine composition for treating dry eyes and visual fatigue, characterized in that: Calculated by weight, the invention comprises 45-55 parts of wild chrysanthemum, 35-45 parts of salvia miltiorrhiza, 8-12 parts of vitex rotundifolia, 8-12 parts of selfheal, 4-8 parts of buddlejac, 10-20 parts of cassia seed, 4-8 parts of coptis root, 5-10 parts of qinpi, 5-8 parts of roxburghii, 10-15 parts of dittany, 0.1-0.5 parts of menthol, 0.1-0.3 parts of borneol, 3-5 parts of enzymatic pearl powder, 2-5 parts of blueberry extract, 1-3 parts of deep-sea whole algae extract and 0.5-2 parts of lutein.

2. The Chinese medicine composition for treating dry eyes and visual fatigue according to claim 1, characterized in that: Calculated by weight, the invention comprises 50-52 parts of wild chrysanthemum, 38-42 parts of salvia miltiorrhiza, 10-12 parts of vitex rotundifolia, 8-10 parts of selfheal, 5-7 parts of buddlejac, 12-16 parts of cassia seed, 5-7 parts of coptis root, 7-8 parts of qinpi, 7-8 parts of roxburghii, 12-14 parts of dittany, 0.2-0.3 parts of menthol, 0.2-0.3 parts of borneol, 4-5 parts of enzymatic pearl powder, 2-3 parts of blueberry extract, 2-3 parts of deep-sea whole algae extract and 1-2 parts of lutein.

3. The Chinese medicine composition for treating dry eye and visual fatigue according to claim 1, characterized in that: Calculated by weight, it includes 50 parts of wild chrysanthemum, 40 parts of salvia miltiorrhiza, 10 parts of vitex rotundifolia, 10 parts of selfheal, 6 parts of buddlejac, 14 parts of cassia seed, 6 parts of coptis chinensis, 7 parts of qinpi, 7 parts of roxburghii, 12 parts of dittany, 0.2 parts of menthol, 0.2 parts of borneol, 4 parts of enzymatic pearl powder, 3 parts of blueberry extract, 2 parts of deep-sea whole algae extract, and 1 part of lutein.

4. A method for preparing the Chinese medicine composition for treating dry eye and visual fatigue according to any one of claims 1 to 3, characterized in that: The steps include: S1: Washing and crushing wild chrysanthemum, salvia miltiorrhiza, vitex rotundifolia, herba prunellae, buddleja mongholicus, cassia seed, coptis root, qinpi, roxburghii and dittany to obtain a mixed medicinal material, adding water to the mixed medicinal material, and performing enzymatic hydrolysis on the mixed medicinal material; S2: sterilizing the mixed medicinal materials after enzymatic hydrolysis, and then fermenting them to obtain a fermentation liquid; S3: inactivating the fermentation liquid, and then performing ultrasonic low-temperature extraction to obtain an extract; S4: extracting the extract to obtain an extract; S5: The extract is concentrated under reduced pressure, and then menthol, borneol, enzymatically hydrolyzed pearl powder, blueberry extract, deep-sea whole algae extract and lutein are added and mixed to obtain a Chinese medicine composition.

5. The method for preparing the Chinese medicine composition for treating dry eye and visual fatigue according to claim 4, characterized in that: In step S1, water and enzymes are added to the mixed medicinal materials to perform enzymatic hydrolysis on the mixed medicinal materials; the enzymes are cellulase, pectinase or neutral protease.

6. The method for preparing the Chinese medicine composition for treating dry eye and visual fatigue according to claim 4, characterized in that: In step S4, ethanol is added to the extract to perform an alcohol extraction to obtain an alcohol extract; then water is added to the extract after the alcohol extraction to perform two water extractions in sequence to obtain a first water extract and a second water extract, respectively; The first water extract and the second water extract are mixed to obtain a mixed solution, ethanol is added to the mixed solution for alcohol precipitation and water extraction, and then the mixed solution is allowed to stand, and the precipitate is removed by filtering to obtain a supernatant; The alcohol extract and the supernatant are mixed to obtain an extract.

7. The method for preparing the Chinese medicinal composition for treating dry eye and visual fatigue according to claim 6, characterized in that: In step S4, the volume concentration of ethanol used in the first alcohol extraction is 60-95%, the volume of ethanol is 3-5 times the volume of the extract, and the processing time of the first alcohol extraction is 1-3h; In the two water extractions, the volume of water is 3-5 times the volume of the extract after the first alcohol extraction, and the treatment time of the two water extractions is 1-2h respectively; In the alcohol precipitation and water extraction, the volume concentration of ethanol added is 90-95%. After the addition of ethanol, the volume concentration of ethanol in the mixed solution is 60-70%, and the standing time is 20-28 hours.

8. Use of the Chinese medicine composition according to any one of claims 1 to 3 or the Chinese medicine composition prepared by the preparation method according to any one of claims 4 to 7 in a non-woven eye patch, characterized in that: Adding glycerin, sodium benzoate and deionized water to the traditional Chinese medicine composition, mixing evenly to obtain an eye patch solution, and immersing a non-woven fabric in the eye patch solution to obtain a non-woven fabric eye patch; The eye patch solution comprises, by weight percentage, 0.1-0.3% of glycerol, 0.2-0.3% of sodium benzoate and 74-75% of deionized water, and the remainder is a traditional Chinese medicine composition.

9. Use of the Chinese medicine composition according to any one of claims 1 to 3 or the Chinese medicine composition prepared by the preparation method according to any one of claims 4 to 7 in a fumigant, characterized in that: Sterile water is added to the traditional Chinese medicine composition, and the mixture is heated to obtain a fumigant; wherein the mass ratio of the traditional Chinese medicine composition to the sterile water is 1:40-1:

50.

10. Use of the Chinese medicine composition according to any one of claims 1 to 3 or the Chinese medicine composition prepared by the preparation method according to any one of claims 4 to 7 in eye ointment or atomized liquid, characterized in that: The application of the Chinese medicine composition in eye ointment comprises: adding medical vaseline, lanolin and liquid wax to the Chinese medicine composition, mixing evenly, and obtaining the eye ointment; The application of the Chinese medicine composition in the atomized liquid comprises: adding sodium hyaluronate and sodium chloride to the Chinese medicine composition, mixing evenly, and filtering to obtain the atomized liquid.

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