A biological attractant and killer for Pomacea canaliculata, its preparation method and application

Abstract

The present invention relates to the field of biological control technology and discloses a bio-attractant for golden apple snails, its preparation method, and application. The bio-attractant comprises 100 parts of croton fruit powder, 100 parts of attapulgite, 3 parts of an adhesive, 700 parts of an attractant, and 100 parts of an auxiliary material. The bio-attractant for golden apple snails of the present invention combines the dual phytotoxicity of the croton fruit with the physiological killing effects of the attapulgite, causing the golden apple snails to die from poisoning and malnutrition after ingesting the food.

Description

Technical Field

[0001] The invention relates to the technical field of biological control, in particular to a bio-attractant for golden apple snails and a preparation method and application thereof. Background Art

[0002] In recent years, due to climate change and improper agricultural water management, the golden apple snail (Pomacea canaliculata) has rapidly spread in rice paddies and wetland environments, posing a serious threat to crop production. The snail feeds on plant stems and leaves, causing plant damage, particularly in rice-growing areas, where it is particularly destructive to crops. Current chemical agents used to control golden apple snails, such as metaldehyde and spirulinamid, are effective but pose environmental pollution risks and are prone to harming non-target organisms. The prior art lacks an environmentally friendly, residue-free, and minimally impactful formulation for controlling golden apple snails.

[0003] In view of the shortcomings of the above-mentioned existing technical solutions, the present invention provides a golden apple snail biological attractant and a preparation method and application thereof. Therefore, it is necessary to develop a golden apple snail biological attractant and a preparation method and application thereof. Summary of the Invention

[0004] The present invention aims to provide a bio-attractant for golden apple snails, its preparation method, and its application, to address the problems of the prior art. The invention aims to effectively control golden apple snails through innovative materials and preparation processes while avoiding environmental pollution caused by chemical agents. To achieve the above-mentioned objectives, the present invention is implemented according to the following technical solutions:

[0005] On the one hand, the present invention comprises the following ingredients: 100 parts of croton fruit powder, 100 parts of attapulgite, 3 parts of adhesive, 700 parts of attractant and 100 parts of auxiliary materials.

[0006] Furthermore, the adhesive is a sodium polyacrylate solution, and the sodium polyacrylate and water are mixed in a ratio of 1:333 to prepare a 0.003 g / mL sodium polyacrylate solution.

[0007] Furthermore, the attractant includes yeast powder, cooked soybean powder, raw soybean powder, corn flour and soybean oil.

[0008] Furthermore, the attractant is composed of cooked soybean powder, raw soybean powder, yeast powder, corn flour and soybean oil in a compound mass ratio of 25:13:2:29:1.

[0009] Furthermore, the auxiliary material comprises wood cellulose, butylparaben and rice straw powder in a mass ratio of 1:1:2.

[0010] A method for preparing a bio-attractant for golden apple snails, used for preparing the bio-attractant for golden apple snails, characterized in that:

[0011] S1. Grind the croton fruit into 40-60 mesh to obtain croton fruit powder;

[0012] S2. Place the attractant, croton fruit powder and sodium polyacrylate aqueous solution in an extrusion granulator for granulation. The granulated particles have a length of 3.0 to 4.0 mm, a width of 2 to 3 mm, and a moisture content of 40%. The particles are dried at below 50°C or air-dried.

[0013] The golden apple snail trapping agent is evenly spread in the irrigation channel of the rice seedling field, with a dosage of 2 to 3 kg per mu of the rice seedling field and 1 kg per m2 of the irrigation area. 2 Apply 60-70g and spread it every 10-20 days.

[0014] The beneficial effects of the present invention are:

[0015] The present invention is a bio-attractant for golden apple snails and its preparation method and application. Compared with the prior art, the present invention has the following technical effects:

[0016] 1. The bio-attractant for golden apple snails of the present invention has a strong feeding effect on golden apple snails, allowing the golden apple snails to actively feed on the bio-attractant.

[0017] 2. The bio-attractant for golden apple snails of the present invention combines the dual effects of the phytotoxicity of croton fruit (croton's toxicity to golden apple snails) and the physiological killing (intestinal obstruction) of attapulgite, causing the golden apple snails to die from poisoning and malnutrition after eating them.

[0018] 3. The bio-attractant for golden apple snails of the present invention is environmentally friendly and free of pesticide residues, and does not pose the risk of excessive pesticide residues in rice and non-point source pollution in the soil. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a comparison diagram of indoor experiments of an implementation case of a golden apple snail biological attractant and a preparation method thereof of the present invention;

[0020] Figure 2 This is a comparison diagram of indoor experiments of an implementation case of a golden apple snail biological attractant and a preparation method thereof of the present invention;

[0021] Figure 3 A comparative diagram of field experiments of an implementation case of a bio-attractant for golden apple snails and a preparation method thereof according to the present invention;

[0022] Figure 4 This is a comparison diagram of field experiments of an implementation case of a golden apple snail biological attractant and a preparation method thereof according to the present invention. DETAILED DESCRIPTION

[0023] The present invention will be further described below through specific examples. The illustrative examples and descriptions of the present invention are used to explain the present invention but are not intended to limit the present invention.

[0024] Example 1 - Indoor toxicity study of preparations with different ratios on Pomacea canaliculata

[0025] Experimental Materials:

[0026] Formula 1: 100g of croton fruit powder, 100g of attapulgite, 700g of attractant, and 100g of auxiliary materials;

[0027] Formula 2: croton powder, attapulgite, sodium polyacrylate, cooked soybean powder, raw soybean powder, corn flour, yeast powder, soybean oil, wood cellulose, butyl paraben, and rice straw powder.

[0028] Formula 3: croton powder, attapulgite, sodium polyacrylate, cooked soybean powder, raw soybean powder, corn flour, yeast powder, soybean oil, wood cellulose, and butylparaben.

[0029] Formula 4: croton powder, sodium polyacrylate, cooked soybean powder, raw soybean powder, corn flour, yeast powder, soybean oil, wood cellulose, butyl paraben, and rice straw powder.

[0030] Formula 5: croton powder, attapulgite, sodium polyacrylate, corn flour, wood cellulose, butyl paraben, and rice straw powder.

[0031] Formula 6: croton powder, attapulgite, sodium polyacrylate, wood cellulose, butyl paraben, and rice straw powder.

[0032] Positive control: an existing chemical control agent for golden apple snails (metaldehyde granules)

[0033] Blank control: A blank control without any pesticide, only feeding the same amount of rice seedlings.

[0034] See Table 1 for specific ingredient contents.

[0035] Table 1 Test formula of bio-attractant for golden apple snail

[0036]

[0037] Example 1 - Laboratory trapping experiment of golden apple snails with different ratios of golden apple snail biological trapping agents

[0038] The tested golden apple snails were Pomacea canaliculata, collected from pollution-free rice fields.

[0039] Experimental Methods: Golden apple snails were cultured in 50 cm × 40 cm × 30 cm polypropylene (PP) plastic boxes at a water depth of 3.5 cm. Ten healthy golden apple snails of equal size (approximately 2.5 cm in length) were placed in each box. Five days prior to the start of the experiment, the snails were starved for four days to empty their intestines. Five replicates were set up for each treatment. Each box in the treatment group contained 0.9 g of a bio-attractant for golden apple snails. The positive control contained 0.15 g of metaldehyde according to the pesticide instructions. The blank control contained 0.9 g of rice seedlings. Following application, the number of dead golden apple snails was observed and recorded daily. Dead snails were promptly removed to prevent water quality problems. Observation was conducted for a total of four days, and the mortality rate of the snails was calculated over the four days. The control efficacy was determined by the difference in mortality between the control group and the treatment group. The results are shown in Table 2.

[0040] Data processing:

[0041] Mortality rate = number of deaths / total number × 100%

[0042] Adjusted mortality rate = (mortality rate of experimental group - mortality rate of control group) / (1 - mortality rate of control group) × 100%

[0043] Table 2 Mortality of apple snails under treatment with bio-attractants in indoor experiments

[0044] serial number 4-day mortality rate (%) 4-day adjusted mortality rate (%) Recipe 1 76±5.48 75±5.71a Recipe 2 8±8.37 14.58±8.72d Recipe 3 48±13.04 45.83±13.58c Recipe 4 62±8.37 60.42±8.72b Recipe 5 42±13.04 39.58±13.58c Recipe 6 22±8.37 18.75±8.72d Positive control 88±8.37 87.5±8.72a Blank control 5±5.48 /

[0045] like Figure 1 and 2 As shown, experiments show that the bio-attractant for golden apple snails of the present invention has the dual effects of physiological killing and plant poisoning. Compared with a single plant extract, the attracting and poisoning effect is significantly improved. Compared with chemical pesticides on the market, it also has good attracting and killing effects, and there is no problem of pesticide residues and non-point source pollution.

[0046] Example 2 - Field trapping experiment of golden apple snails with different ratios of golden apple snail biological trapping agents

[0047] Test materials: Same as Example 1

[0048] Experimental method: The experiment was conducted in a rice seedling field in June 2024. A total of 8 treatments were set up, each treatment had 3 replicates (3 plots), a total of 24 plots, each plot was 2m 2The plots were randomly distributed in 2 m × 1 m squares. Ridges were constructed in each plot and protected with protective netting to prevent the escape of golden apple snails. Ten healthy golden apple snails of equal size (approximately 2.5 cm in length) were evenly distributed in each plot. A bio-attractant for golden apple snails was applied uniformly to each plot at 9 g. A positive control was administered with 1.5 g of metaldehyde granules per plot according to the pesticide instructions. A blank control group was left untreated. Four days after application, the mortality and adjusted mortality of the golden apple snails were measured. The control efficacy was determined by the difference in mortality between the control and treatment groups. The calculated results are shown in Table 3.

[0049] Data processing:

[0050] Mortality rate = number of deaths / total number × 100%

[0051] Adjusted mortality rate = (mortality rate of experimental group - mortality rate of control group) / (1 - mortality rate of control group) × 100%

[0052] Table 3 Mortality of apple snails treated with bio-attractants in field experiments

[0053]

[0054]

[0055] like Figure 3 and 4 As shown, experiments show that when the biocontrol agent of the present invention is used in the field, the probability of attracting and killing snails is reduced compared to indoor use due to the complex chemical substances in paddy fields. The killing effect of Formulation 1 is significantly improved compared to the non-attractant treatment of Formulation 6. The killing effect of the golden apple snail using the bio-attractant of Formulation 1 is not much different from that of metaldehyde granules.

[0056] The foregoing description is merely a preferred embodiment of the present invention. It should be understood that the present invention is not limited to the form disclosed herein and should not be construed as excluding other embodiments. Rather, the present invention can be used in various other combinations, modifications, and environments and can be modified within the scope of the concepts described herein by utilizing the above description or related art techniques. Modifications and variations made by those skilled in the art that do not depart from the core technology and scope of the present invention should be within the scope of protection of the appended claims.

Claims

1. A bio-attractant for golden apple snails, characterized in that: The invention comprises the following ingredients: 100 parts of croton fruit powder, 100 parts of attapulgite, 3 parts of adhesive, 700 parts of attractant and 100 parts of auxiliary material, wherein the adhesive is sodium polyacrylate solution, and sodium polyacrylate and water are mixed in a ratio of 1:333 to prepare a 0.003 g / mL sodium polyacrylate solution; the attractant comprises yeast powder, cooked soybean powder, raw soybean powder, corn flour and soybean oil; and the auxiliary material comprises wood cellulose, butyl paraben and rice straw powder in a mass ratio of 1:1:

2.

2. A bio-attractant for golden apple snails according to claim 1, characterized in that: The attractant consists of cooked soybean powder, raw soybean powder, yeast powder, corn flour and soybean oil in a compound mass ratio of 25:13:2:29:

1.

3. A method for preparing a bio-attractant for golden apple snails, for preparing the bio-attractant for golden apple snails according to any one of claims 1 to 2, characterized in that: S1. Grind the croton fruit into 40-60 mesh to obtain croton fruit powder; S2. Place the attractant, croton fruit powder and sodium polyacrylate aqueous solution in an extrusion granulator for granulation. The granulated particles have a length of 3.0 to 4.0 mm, a width of 2 to 3 mm, and a moisture content of 40%. The particles are dried at below 50°C or air-dried.

4. Use of the bio-attractant for controlling golden apple snails in rice fields and wetlands according to any one of claims 1 to 2, characterized in that: The golden apple snail trapping agent is evenly spread in the irrigation channel of the rice seedling field, with a dosage of 2 to 3 kg per mu of the rice seedling field and 1 kg per m2 of the irrigation area. 2 Apply 60-70g and spread it every 10-20 days.

Images