A hand sanitizer without washing and its preparation method

Abstract

The present invention relates to the technical field of hand sanitizers, and particularly relates to a hand sanitizer without rinsing and a preparation method thereof. The hand sanitizer without rinsing is composed of the following components by mass percentage: 0.5-1% of modified soy peptide, 0.8-1.2% of Artemisia japonica polysaccharide, 1-3% of glycerol, 0.3-0.5% of carbomer, 0.05-0.15% of triethanolamine, 0.1-0.5% of vitamin E, 0.02-0.05% of rose essence, and the balance is distilled water; the structural formula of the modified soy peptide is as follows: The hand sanitizer without rinsing provided by the present invention has excellent bactericidal effect and long action time, and is mild, skin-friendly and non-irritating.

Description

Technical Field

[0001] The present invention relates to the technical field of hand sanitizers, and particularly to a hand sanitizer without rinsing and a preparation method thereof. Background Art

[0002] With the increasing attention of people to health and the improvement of environmental protection awareness, hand sanitizers without rinsing are more and more popular among people. As a hand cleaning and disinfection product, the characteristic of hand sanitizers without rinsing is that they can achieve the effects of cleaning and disinfection without using water sources, and are very convenient to use, and are widely used in various occasions such as hospitals, banks, supermarkets, and tourist scenes.

[0003] At present, the common hand sanitizers without rinsing on the market usually use ethanol, cationic surfactants or triclosan as antibacterial components, and at the same time add moisturizing components such as glycerol. Such hand sanitizers without rinsing have the advantages of good antibacterial effect and low cost. However, the evaporation rate of ethanol is fast, which will not only accelerate the loss of skin moisture and stimulate the skin, but also be difficult to have a long-term antibacterial effect. Therefore, it is particularly important to develop a green and safe alcohol-free hand sanitizer without rinsing with good antibacterial effects. Summary of the Invention

[0004] In order to overcome the deficiencies of the prior art, one of the purposes of the present invention is to provide a hand sanitizer without rinsing.

[0005] Another purpose of the present invention is to provide a preparation method of a hand sanitizer without rinsing.

[0006] One of the purposes of the present invention is achieved by adopting the following technical solutions:

[0007] A hand sanitizer without rinsing is composed of the following components by mass percentage: 0.5 - 1% of modified soy peptide, 0.8 - 1.2% of artemisia japonica polysaccharide, 1 - 3% of glycerol, 0.3 - 0.5% of carbomer, 0.05 - 0.15% of triethanolamine, 0.1 - 0.5% of vitamin E, 0.02 - 0.05% of rose essence, and the balance is distilled water; the structural formula of the modified soy peptide is as follows:

[0008] .

[0009] Further, the preparation method of the modified soy peptide is as follows:

[0010]

[0011] (1) Dissolve soy peptide in water to obtain a soy peptide solution, add glycidyl methacrylate, triethylamine and tetrabutylammonium bromide, heat and react, then add absolute ethanol for precipitation, and obtain compound A after centrifugation;

[0012] (2) Add compound A to water and stir evenly. Then add 4-formylphenylboronic acid for reaction. After the reaction is completed, transfer the reaction solution into a dialysis bag. After dialysis, freeze-dry to obtain modified soy peptide.

[0013] Further, in step (1), the dosage ratio of the soy peptide, glycidyl methacrylate, triethylamine, and tetrabutylammonium bromide is 5 g : 1 - 2 mL : 0.5 - 1.5 mL : 2 - 3 g; the concentration of the soy peptide solution is 0.01 g / mL.

[0014] Further, in step (1), the temperature of the heating reaction is 45 - 55 °C, and the time is 22 - 26 h.

[0015] Further, in step (2), the mass ratio of compound A to 4-formylphenylboronic acid is 1.5 : 0.15 - 0.3, and the concentration of compound A in water is 0.01 g / mL.

[0016] Further, in step (2), the reaction time is 24 - 28 h.

[0017] Further, in step (2), the cut-off molecular weight of the dialysis bag is 2 - 5 kDa.

[0018] Further, the preparation of the soy peptide includes the following steps:

[0019] Mix soy protein isolate with phosphate buffer solution, add trypsin for enzymatic hydrolysis. After the enzymatic hydrolysis is completed, inactivate the enzyme at 90 °C for 15 min, adjust the pH to neutral and then centrifuge to obtain soy peptide.

[0020] A preparation method of the above-mentioned hand sanitizer without washing includes the following steps:

[0021] Add the modified soy peptide, Artemisia japonica polysaccharide, and carbomer into water according to the above weight parts, stir until fully dissolved, add triethanolamine to adjust the pH, and then add glycerol, vitamin E, and rose essence, and mix evenly to obtain.

[0022] Further, the pH is 6.5 - 6.8.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0024] 1. The present invention provides a hand sanitizer. By adding modified soy peptide and Artemisia japonica polysaccharide, this hand sanitizer has excellent bactericidal effect and long action time, and is mild, skin-friendly and non-irritating. The modified soy peptide undergoes a ring-opening reaction with the epoxy group of glycidyl methacrylate to obtain compound A. Subsequently, the amino group of compound A forms a Schiff base with 4-formylphenylboronic acid, introducing both antibacterial boronic acid groups and enhancing film-forming property, thus achieving long-acting antibacterial and skin-friendly moisturizing. As a natural extract, Artemisia japonica polysaccharide can reduce the irritation of boronic acid groups and synergistically enhance the antibacterial and moisturizing properties in the hand sanitizer.

[0025] 2. The present invention provides a preparation method of a hand sanitizer. This method is simple and feasible, providing a new idea for the preparation of a new type of hand sanitizer. Detailed implementation manners

[0026] Next, in combination with the detailed implementation manners, the present invention will be further described. It should be noted that, on the premise of no conflict, the following-described embodiments or technical features can be arbitrarily combined to form new embodiments. For the specific conditions not specified in the embodiments, they are carried out according to the conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are all conventional products obtained through commercial channels.

[0027] The preparation process of soy peptide in the present invention includes the following steps:

[0028] Mix soy protein isolate and phosphate buffer solution, where the mass fraction of soy protein isolate is 5%. Then add trypsin to it, and the enzyme addition amount of trypsin is 6% of the substrate; adjust the pH to 8.0, then carry out enzymatic hydrolysis at 45 °C for 4 h, heat to 90 °C for 15 min to inactivate the enzyme, adjust the pH value of the enzymatic hydrolysate to 7.0, then centrifuge at 5000 r / min for 20 min, take the supernatant, and freeze-dry to obtain soy peptide.

[0029] The preparation process of Artemisia japonica polysaccharide in the present invention includes the following steps:

[0030] (1) Place the dry leaves of Artemisia japonica in a container, add 10 times the weight of Artemisia japonica of 95% ethanol and decoct twice, each time for 3 h, to obtain defatted Artemisia japonica;

[0031] (2) Add an equal amount of distilled water to the above defatted Artemisia japonica and decoct 3 times, each time for 3 h. Combine the 3 portions of decoction obtained and concentrate to 40% - 50% of its volume. Add 3 times the volume of 95% ethanol to precipitate the product, filter to obtain the solid product, and dry to obtain Artemisia japonica polysaccharide.

[0032] Example 1

[0033] A hand sanitizer without rinsing is composed of the following components by mass percentage: modified soy peptide 0.75%, Artemisia japonica polysaccharide 1%, glycerol 2%, carbomer 0.4%, triethanolamine 0.10%, vitamin E 0.3%, rose essence 0.04%, and the balance is distilled water; the structural formula of the modified soy peptide is as follows:

[0034] 。

[0035] The preparation method of the modified soy peptide is as follows:

[0036]

[0037] (1) Dissolve soy peptide in water to obtain a soy peptide solution, add glycidyl methacrylate, triethylamine and tetrabutylammonium bromide, and react at 50 °C for 24 h. The dosage ratio of soy peptide, glycidyl methacrylate, triethylamine and tetrabutylammonium bromide is 5 g: 1.5 mL: 1 mL: 2.5 g; the concentration of the soy peptide solution is 0.01 g / mL; add anhydrous ethanol for precipitation, and obtain compound A after centrifugation;

[0038] (2) Add compound A to water and stir evenly, add 4-formylphenylboronic acid and react at room temperature for 26 h. The mass ratio of compound A to 4-formylphenylboronic acid is 1.5: 0.2, and the concentration of compound A in water is 0.01 g / mL; after the reaction is completed, transfer the reaction solution to a dialysis bag (cut-off molecular weight is 2 kDa), and freeze-dry after dialysis to obtain the modified soy peptide.

[0039] Example 1 also provides a preparation method of the above hand sanitizer without rinsing, including the following steps:

[0040] Add the modified soy peptide, Artemisia japonica polysaccharide and carbomer into water according to the above weight parts, stir until completely dissolved, add triethanolamine to adjust the pH to 6.5, and then add glycerol, vitamin E and rose essence, and mix evenly to obtain.

[0041] Example 2

[0042] A hand sanitizer without rinsing is composed of the following components by mass percentage: modified soy peptide 0.5%, Artemisia japonica polysaccharide 0.8%, glycerol 1%, carbomer 0.3%, triethanolamine 0.05%, vitamin E 0.1%, rose essence 0.02%, and the balance is distilled water; the structural formula of the modified soy peptide is the same as that in Example 1.

[0043] The preparation method of the modified soy peptide is as follows:

[0044] (1) Dissolve soy peptide in water to obtain a soy peptide solution. Add glycidyl methacrylate, triethylamine, and tetrabutylammonium bromide and react at 45 °C for 26 h. The dosage ratio of soy peptide, glycidyl methacrylate, triethylamine, and tetrabutylammonium bromide is 5 g: 1 mL: 0.5 mL: 2 g; the concentration of the soy peptide solution is 0.01 g / mL; add absolute ethanol for precipitation, and obtain compound A after centrifugation;

[0045] (2) Add compound A to water and stir evenly. Add 4-formylphenylboronic acid and react at room temperature for 24 h. The mass ratio of compound A to 4-formylphenylboronic acid is 1.5: 0.15, and the concentration of compound A in water is 0.01 g / mL; after the reaction is completed, transfer the reaction solution to a dialysis bag (cut-off molecular weight is 3 kDa), and freeze-dry after dialysis to obtain modified soy peptide.

[0046] Example 2 also provides a preparation method of the above-mentioned hand sanitizer without washing, including the following steps:

[0047] Add the modified soy peptide, Artemisia japonica polysaccharide, and carbomer to water according to the above weight parts, stir until fully dissolved, add triethanolamine to adjust the pH to 6.7, and then add glycerol, vitamin E, and rose essence, and mix evenly to obtain.

[0048] Example 3

[0049] A hand sanitizer without washing is composed of the following components by mass percentage: 1% of modified soy peptide, 1.2% of Artemisia japonica polysaccharide, 3% of glycerol, 0.5% of carbomer, 0.15% of triethanolamine, 0.5% of vitamin E, 0.05% of rose essence, and the balance is distilled water; the structural formula of the modified soy peptide is the same as that in Example 1.

[0050] The preparation method of the modified soy peptide is as follows:

[0051] (1) Dissolve soy peptide in water to obtain a soy peptide solution. Add glycidyl methacrylate, triethylamine, and tetrabutylammonium bromide and react at 55 °C for 26 h. The dosage ratio of soy peptide, glycidyl methacrylate, triethylamine, and tetrabutylammonium bromide is 5 g: 2 mL: 1.5 mL: 3 g; the concentration of the soy peptide solution is 0.01 g / mL; add absolute ethanol for precipitation, and obtain compound A after centrifugation;

[0052] (2) Add compound A to water and stir evenly. Add 4-formylphenylboronic acid and react at room temperature for 28 h. The mass ratio of compound A to 4-formylphenylboronic acid is 1.5: 0.3, and the concentration of compound A in water is 0.01 g / mL; after the reaction is completed, transfer the reaction solution to a dialysis bag (cut-off molecular weight is 5 kDa), and freeze-dry after dialysis to obtain modified soy peptide.

[0053] This embodiment 3 also provides a method for preparing the above-mentioned hand sanitizer, comprising the following steps:

[0054] Add the modified soybean peptide, artemisia polysaccharide and carbomer into water according to the above weight parts, stir until fully dissolved, add triethanolamine to adjust the pH to 6.8, then add glycerol, vitamin E and rose essence, mix well, and obtain the product.

[0055] Comparative Example 1

[0056] This comparative example 1 is basically the same as example 1, except that the modified soybean peptide is replaced by soybean peptide.

[0057] Comparative Example 2

[0058] This comparative example 2 is basically the same as example 1, except that the modified soybean peptide is replaced by compound A obtained in step (1).

[0059] Comparative Example 3

[0060] This comparative example 3 is substantially the same as Example 1, except that artemisia japonica polysaccharide is omitted.

[0061] Effect example

[0062] 1. Antibacterial test

[0063] In order to evaluate the long-term antibacterial effect of the hand sanitizer of the present invention, the following test was performed, and the specific steps were as follows: healthy female volunteers aged 25-40 were selected as subjects and randomly divided into Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group, with 10 people in each group. The left and right hands of the subjects were sampled by the cotton test smearing method as a control group before the experiment. Take 2mL of the hand sanitizer prepared by Examples 1-3 and Comparative Examples 1-3 and evenly apply it to both hands (such as Example 1 group, the hand sanitizer prepared by Example 1 used), and then rub the hands for 2 minutes according to the 6-step method. Then, at 2h, 6h, and 8h after rubbing the hands, the fingers of each group of subjects were sampled by the cotton test smearing method. The specific sampling method of the above cotton swab smear method is as follows: the subject puts five fingers together, soaks the sterile cotton swab in sterile saline, and rubs it twice on the curved surface of the fingers of both hands (30 cm² for each hand) from the base to the fingertips. After rotating the swab, cut off the part that contacts the hand of the sampler, put the cotton swab into a test tube containing a neutralizer and vibrate 80 times for neutralization for 10 minutes. Take 0.5mL of the test solution and add it to a 4.5mL nutrient broth tube, incubate it in a 37℃ incubator for 48h, and calculate the total number of bacterial colonies per square centimeter of the hand. The results are detailed in Table 1. Antibacterial rate = (total number of colonies in the control group before the experiment - number of bacterial colonies in each group after different time of action) / total number of colonies in the control group before the experiment × 100%.

[0064] Table 1 Antibacterial effect of hand sanitizers in each group

[0065]

[0066] According to the test results in Table 1, the antibacterial effect of the hand sanitizers in Examples 1-3 of the present invention is better than that in Comparative Examples 1-3, and still has a good antibacterial effect after 8 hours of action, and the antibacterial rate is still above 93%. In Comparative Example 1, the modified soy peptide was replaced with soy peptide. In Comparative Example 2, the modified soy peptide was replaced with compound A prepared in step (1). In Comparative Example 3, Artemisia japonica polysaccharide was omitted. The antibacterial effects of Comparative Example 2 and Comparative Example 3 were slightly higher than that of Comparative Example 1, but both were lower than that of Example 1. This is mainly due to the addition of the modified soy peptide, indicating that the modified soy peptide has a good antibacterial effect and can inhibit the reproduction of bacteria. This is because the carboxyl group in the modified soy peptide undergoes a ring-opening reaction with the epoxy group in glycidyl methacrylate to obtain compound A, and then a Schiff base reaction occurs between the amino group on the soy peptide in compound A and 4-formylphenylboronic acid. The introduced borate group enhances the antibacterial property of the hand sanitizer, and the introduction of methacrylate improves the film-forming property of the hand sanitizer, making it easy to form a film on the hand and not easy to lose, thus achieving the effect of long-term antibacterial. In addition, the addition of Artemisia japonica polysaccharide in the hand sanitizer reduces the irritation of the borate group in the modified soy peptide to the skin, and can also act synergistically with the modified soy peptide to enhance the antibacterial performance of the hand sanitizer without washing.

[0067] 2. Moisture retention effect test

[0068] Healthy female volunteers aged 25-40 years old were selected and randomly divided into Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group, with 5 people in each group. After the inner side of the left arm of each group of volunteers was washed with clean water and dried with sterile paper, a 4 cm × 4 cm area on the inner side of the left arm was selected as the test area. Then, a skin moisture meter was used to measure the water content of the stratum corneum in this area (i.e., the water content before the test). Then, 0.5 mL of the hand sanitizer corresponding to the group was applied (for example, the hand sanitizer without washing prepared in Example 1 was applied to the Example 1 group), and applied until evenly absorbed. After 30 minutes, the water content of the stratum corneum in this area was measured, and the test results were recorded in Table 2.

[0069] Table 2 Moisture retention effect of hand sanitizers in each group

[0070]

[0071] As can be seen from the test results in Table 2, the hand sanitizers of Examples 1-3 of the present invention have good moisturizing effects. Compared with Example 1, the moisturizing effect of Comparative Example 3 decreased significantly after Artemisia japonica polysaccharide was omitted. The moisturizing effect of Comparative Example 2 was slightly better than that of Comparative Example 1. This may be because compound A in Comparative Example 2 introduced methyl methacrylate, improving the film-forming property of the hand sanitizer, making it difficult for moisture to escape, and thus improving the moisturizing effect of the hand sanitizer.

[0072] The above embodiments are only the preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantive changes and substitutions made by those skilled in the art based on the present invention fall within the scope of protection required by the present invention.

Claims

1. A hand sanitizer, characterized in that: The composition is composed of the following ingredients by mass percentage: 0.5-1% modified soybean peptide, 0.8-1.2% artemisia polysaccharide, 1-3% glycerol, 0.3-0.5% carbomer, 0.05-0.15% triethanolamine, 0.1-0.5% vitamin E, 0.02-0.05% rose essence, and the balance is distilled water; the structural formula of the modified soybean peptide is as follows: ； The preparation method of the modified soybean peptide is as follows: (1) dissolving soybean peptide in water to obtain a soybean peptide solution, adding glycidyl methacrylate, triethylamine and tetrabutylammonium bromide to heat for reaction, adding anhydrous ethanol to precipitate, and centrifuging to obtain compound A; (2) Compound A is added into water and stirred evenly, and 4-formylphenylboronic acid is added to react. After the reaction is completed, the reaction solution is transferred into a dialysis bag, and after dialysis, freeze-dried to obtain a modified soybean peptide.

2. The hand sanitizer according to claim 1, characterized in that: In step (1), the soybean peptide, glycidyl methacrylate, triethylamine and tetrabutylammonium bromide are used in a ratio of 5 g: 1-2 mL: 0.5-1.5 mL: 2-3 g; The concentration of the soybean peptide solution is 0.01 g / mL.

3. The hand sanitizer according to claim 1, characterized in that: In step (1), the heating reaction temperature is 45-55°C and the time is 22-26 hours.

4. The hand sanitizer according to claim 1, characterized in that: In step (2), the mass ratio of the compound A to 4-formylphenylboronic acid is 1.5:0.15-0.3, and the concentration of the compound A in water is 0.01 g / mL.

5. The hand sanitizer according to claim 1, characterized in that: In step (2), the reaction time is 24-28 hours.

6. The hand sanitizer according to claim 1, characterized in that: In step (2), the molecular weight cutoff of the dialysis bag is 2-5 kDa.

7. The hand sanitizer according to claim 1, characterized in that: The preparation of the soybean peptide comprises the following steps: The soybean protein isolate was mixed with phosphate buffer, and trypsin was added for enzymolysis. After the enzymolysis, the enzyme was inactivated at 90°C for 15 minutes, the pH was adjusted to neutral, and then centrifuged to obtain soybean peptides.

8. A method for preparing the hand sanitizer according to any one of claims 1 to 7, characterized in that: The steps include: Add the modified soybean peptide, artemisia polysaccharide and carbomer into water according to the above weight parts, stir until fully dissolved, add triethanolamine to adjust the pH, then add glycerol, vitamin E and rose essence, mix well, and obtain the product.

9. The method for preparing the hand sanitizer according to claim 8, characterized in that: The pH is 6.5-6.8.

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