Wulian yinao tong for relieving ischemic stroke and its preparation method

Abstract

The application discloses a Wulian Yinao Tong for relieving ischemic stroke, which comprises the following components: Euonymus fortunei, Lysidice rhodostegia, Spatholobus suberectus, Smilax glabra, Tylopoda tridentata, Millettia pinnata, Ficus tikoua and Panax notoginseng. Compared with the prior art, the Wulian Yinao Tong is prepared by analyzing the etiology and pathogenesis of ischemic stroke according to the theory of Mongolian medicine, and the S. glabra is used for dispelling wind, the L. rhodostegia is used for tonifying qi and nourishing blood, and the S. suberectus is used for activating blood and tonifying blood, so that the three components are used as the main medicine to jointly achieve the effects of dispelling wind and removing toxin, regulating qi movement, promoting the synchronization of three qi, dredging the two channels and three collaterals by qi carrying blood, reducing the content of ferrous ions and the water content in blood, increasing the content of glutathione, and reducing the ratio of cerebral infarction.

Description

Technical Field

[0001] The present invention relates to the field of traditional Chinese medicine, and in particular to Wulian Yinaotong for alleviating ischemic stroke and a preparation method thereof. Background Art

[0002] Ischemic stroke occurs when a blockage in a blood vessel causes insufficient blood supply to a certain area of ​​the brain. This insufficient blood supply leads to ischemic damage or necrosis of brain tissue, resulting in irreversible neurological impairment. Ischemic stroke can cause varying degrees and types of brain damage, including brain tissue lesions and neuronal death.

[0003] Because ischemic stroke can be associated with coronary atherosclerosis, cardiac factors, and arteriolar occlusion, patients often experience symptoms such as limb numbness, dizziness, and headaches. They may also experience speech disorders and dysphagia. It is the second leading cause of death worldwide and one of the conditions with the highest disability rates. Furthermore, once an ischemic stroke occurs, the risk of recurrence is high, typically requiring long-term medication to control the condition and prevent recurrence. Furthermore, because ischemic stroke is often associated with conditions such as hypertension and hyperlipidemia, Western medicine for prevention and treatment requires concurrent use of multiple medications, potentially increasing the burden on the patient's internal organs. Summary of the Invention

[0004] The purpose of this application is to provide a Wulian Yinaotong for alleviating ischemic stroke.

[0005] The purpose of this application is also to provide a preparation method of Wulian Yinaotong for alleviating ischemic stroke.

[0006] To achieve the above objectives, the present application provides a Wulian Yinaotong for alleviating ischemic group, wherein the Wulian Yinaotong includes Fufang Teng, Huanghua Daoshuilian, Millettia repens, Kuanjinteng, Zoumatai, Niu Dali, Wuzhimaotao and Panax notoginseng.

[0007] Preferably, in the above technical solution, the raw materials of the Wulian Yinaotong include, by weight, 10-20g of Fufang Caulis, 10-20g of Wuzhimao Peach, 10-20g of Millettia reticulata, 5-15g of Dianthus grandiflora, 10-20g of Kuanjin Caulis, 5-15g of Zoumatai, 10-20g of Huanghua Daoshuilian and 5-15g of Panax notoginseng.

[0008] Preferably, in the above technical solution, the raw materials of the Wulian Yinaotong include, by weight, 13-17g of Fufang Caulis, 13-17g of Wuzhimao Peach, 13-17g of Millettia reticulata, 8-12g of Dianthus grandiflora, 13-17g of Kuanjin Caulis, 8-12g of Zoumatai, 13-17g of Huanghua Daoshuilian and 8-10g of Panax notoginseng.

[0009] Preferably, in the above technical solution, the raw materials of the Wulian Yinaotong include, by weight, 15g of Fufang Caulis, 15g of Wuzhimi Cai, 15g of Millettia reticulata, 10g of Niu Dali, 15g of Kuanjin Caulis, 10g of Zoumatai, 15g of Huanghuai Lian and 9g of Panax notoginseng.

[0010] Preferably, in the above technical solution, the Wulian Yinaotong also includes Prunella vulgaris and earthworm.

[0011] Preferably, in the above technical scheme, the raw materials of the Wulian Yinaotong include, by weight, 10-20g of Fufang Caulis, 10-20g of Wuzhimao Peach, 10-20g of Millettia reticulata, 5-15g of Niu Dali, 10-20g of Kuanjin Caulis, 5-15g of Zoumatai, 10-20g of Huanghua Daoshuilian, 5-15g of Panax notoginseng, 5-15g of earthworm and 5-15g of Prunella Vulgaris.

[0012] Preferably, in the above technical solution, the raw materials of the Wulian Yinaotong include, by weight, 15g of Fufang Caulis, 15g of Wuzhimi Cai, 15g of Millettia reticulata, 10g of Niu Dali, 15g of Kuanjin Caulis, 10g of Zoumatai, 15g of Huanghua Daoshuilian, 9g of Panax notoginseng, 10g of earthworm and 10g of Prunella vulgaris.

[0013] A preparation method for alleviating ischemic group Wulian Yinaotong, the preparation method comprising:

[0014] S1, cut Millettia spatholobi, Euphorbia fangyuan, Cistanche deserticola, Cynanchum indica, and Cynanchum lappa into small pieces, grind Panax notoginseng into powder, and weigh out Cistanche deserticola, Cistanche deserticola, Cistanche deserticola, Cynanchum indica, Cynanchum lappa, and Panax notoginseng powder;

[0015] S2. Soak Euonymus fortunei, Eleutherococcus quinquefolius, Millettia repens, Cynanchum wilfordii, Cynanchum lappa, and Trametes zognathifolia in 500-800 mL of water for 20-40 min, boil over high heat, and simmer over low heat for 10-20 min. Add Rhizoma Coptidis and Panax notoginseng powder, simmer over low heat for 10-20 min, filter, and obtain the medicinal liquid to prepare Wulian Yinaotong.

[0016] Preferably, in the above technical solution, the part of the medicinal materials includes five or more of the following: Eucommia ulmoides, Eleutherococcus chinensis, Millettia repens, Cynanchum wilfordii, Cynanchum chinense, Cynanchum chinense, Pteris parviflora, Pheretima chinensis and Prunella vulgaris.

[0017] Preferably, in the above technical solution, the Wulian Yinaotong is an oral medicine, and the method of taking it is to take it warm once in the morning, noon and evening.

[0018] The medicinal properties and efficacy of the medicinal materials used in the present invention are as follows:

[0019] Euonymus fortunei (Turcz.) Hand.-Mazz is an evergreen vine shrub of the Euonymus family. Also known as golden thread wind, nine-ox-plant, wall-leaning wind, trachelospermum vine, and wall-climbing grass, it has a bitter and sweet flavor with a slightly pungent and warm nature. It has the effects of enhancing the body's immune function, strengthening the heart, stopping bleeding, preventing thrombosis, relaxing muscles and tendons, and promoting qi and blood circulation. It is used to treat low back and knee pain, hemiplegia, and rheumatic pain.

[0020] Five-fingered ginseng (Ficus simplicissima Lour.), also known as five-finger milk, five-finger incense, five-finger banyan, five-claw dragon, and sow's milk, is the dried root of the Ficus simplicissima plant in the Moraceae family. It has a sweet taste and a neutral nature, and enters the lung, spleen, and liver meridians. It strengthens the spleen and removes dampness, promotes blood circulation and removes blood stasis, dispels cold, and relieves pain. It is primarily used to treat symptoms such as edema due to spleen deficiency, poor appetite, cough due to tuberculosis, night sweats, leucorrhea, postpartum agalactia, rheumatic pain, edema, ascites due to cirrhosis, hepatitis, and traumatic injuries.

[0021] Spatholobus suberectus Dunn (also known as Blood Wind Vine, Blood Wind, Blood Vine, Three-leaf Spatholobus, and Nine-layer Wind) is a vine from the Leguminosae family, including Spatholobus suberectus, Spatholobus suberectus, Spatholobus suberectus, and Spatholobus suberectus. It has a bitter and sweet flavor and warm properties; it enters the Liver and Kidney meridians. Spatholobus suberectus has the effects of promoting blood circulation, tonifying the muscles and tendons, regulating menstruation, and relieving pain. It is primarily used to treat low back and knee pain, numbness and paralysis, rheumatic pain, blood deficiency, chlorosis, and irregular menstruation.

[0022] Millettia speciosa Champ., also known as the beautiful millettia vine, mountain lotus root, and powerful potato, is sweet and neutral in nature. It enters the lung and kidney meridians. It nourishes and moistens the lungs, strengthens tendons, and activates collaterals. It is primarily used to treat lumbar muscle strain, rheumatoid arthritis, lung heat, lung deficiency cough, tuberculosis, chronic bronchitis, chronic hepatitis, and spermatorrhea.

[0023] Kuanjinteng (Songgenteng), also known as Songgenteng, Shujinteng, and Dasongshen, is the stem of the Chinese twig of the Menispermaceae family. It has a bitter taste and cooling properties, and enters the Liver meridian. Kuanjinteng has the effects of relaxing tendons and activating collaterals, dispelling wind and relieving pain. It is primarily used to treat rheumatic pain, sciatica, lumbar muscle strain, and traumatic injuries.

[0024] Radix Ardisiae Gigantifoliae, also known as Zoumafeng, Zoumateng, and Dafayao, is the dried root of the Myrsinaceae plant. It has a pungent and bitter flavor and warm properties. It enters the lung, liver, and stomach meridians. It dispels wind and activates blood circulation, strengthens tendons and bones, disperses blood stasis and reduces swelling, and relieves toxins and pain. It is primarily used to treat generalized pain caused by colds, headaches, and rheumatic joint and muscle pain.

[0025] Polygala fallax Hemsl. is the dried root of Polygala fallax, a Polygala plant in the family Polygalaceae. It has a sweet taste and a neutral nature, and enters the liver, kidney, and spleen meridians. It has the benefits of invigorating qi and blood, strengthening the spleen and removing dampness, promoting blood circulation and regulating menstruation, strengthening the body, removing dampness and dispersing blood stasis, and replenishing qi and blood. It is primarily used to treat lumbar disc herniation, fatigue, and coronary artery resistance affecting myocardial function.

[0026] Panax notoginseng (Burk.) FHChen is the dried root of the Araliaceae plant, also known as Tianqi and Jinbuhuan. It has a sweet, slightly bitter flavor and a warm nature. It enters the Liver and Stomach meridians. It promotes blood circulation and removes blood stasis, improves cardiovascular disease, nourishes the blood, enhances immunity, and relieves pain. It is primarily used to treat coronary heart disease, hypertension, migraines, chronic atrophic gastritis, and acute cardiovascular and cerebrovascular diseases.

[0027] Prunella vulgaris (scientific name: Prunella vulgaris), a plant of the genus Prunella in the Lamiaceae family, is also known as Nine-story Tower, Iron-colored Grass, Big Head Flower, Stick-shaped Flower, and Sheep Intestine Vegetable. It has a bitter and pungent flavor and a cold nature; it enters the Liver and Gallbladder Meridians. It has the effects of clearing the liver and improving eyesight, dispersing stagnation and reducing swelling, clearing away heat and detoxifying, and lowering blood pressure. It is primarily used to treat red, swollen, and painful eyes, headaches, dizziness, and night pain in the eyes.

[0028] Earthworms (Pheretima) are the dried, whole body of the earthworm Pheretima spp. (Earthworms), also known as Pheretima spp., a member of the family Giganticus. They have a salty flavor and cold properties, and enter the liver, spleen, and bladder meridians. They dispel wind, clear heat, calm spasms, dredge meridians, relieve asthma, and act as a diuretic. They are primarily used to treat rheumatic pain, hemiplegia, and heat-induced convulsions.

[0029] Compared with the prior art, this application has the following beneficial effects:

[0030] (1) This prescription is based on the analysis of the etiology of ischemic stroke according to Zhuang medicine theory: the three qi are out of sync, the airway is blocked, causing qi deficiency and fatigue, qi stagnation and blood deficiency lead to poor blood circulation, the three channels and two roads are blocked, the three channels and the dragon road are blocked, the nutrient delivery of the internal organs, bones and flesh is blocked, the internal organs are deficient and weak, the qi flow is weak, the blood flow is stagnant, and the vascular embolism is aggravated, and the three dragon roads affect the nerve information transmission and are blocked; if the external evil invades occasionally, it causes the qi and blood to be disordered, and the blood rushes to the brain, which is easy to cause stroke and fainting. The treatment mechanism is: dispelling wind, replenishing qi, regulating qi, and promoting qi movement, regulating qi, synergistically nourishing blood, activating blood circulation, and promoting smooth blood circulation, so as to achieve the effect of unblocking the three channels and two roads, harmonizing qi and blood, balancing yin and yang, and synchronizing the three qi.

[0031] (2) The formula of this group of prescriptions is explained as follows: Kuanjinteng dispels wind, Huanghuadaoshuilian replenishes qi and nourishes blood, and Jixueteng activates blood circulation and replenishes blood as the main medicine, dispels wind and removes toxins, regulates qi, promotes the synchronization of the three qi, and carries blood to clear the two paths and three channels; Zoumatai activates blood circulation, Niu Dali replenishes qi and nourishes blood, Sanqi replenishes blood and nourishes blood, and Fufangteng promotes qi and blood circulation as the auxiliary medicine, strengthening the main medicine's qi carrying blood circulation and promoting the circulation of qi and blood to the bones and flesh. Wuzhimaotao leads all the medicines directly to the diseased area and regulates the two paths, thus becoming the supporting medicine. The eight medicines work together to regulate qi and blood, unblock the three paths and two channels, and regulate the three qi in synchronization. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings and in conjunction with the embodiments.

[0033] Figure 1 is a graph of the standard curve for ferrous ion;

[0034] Figure 2 is a graph of the glutathione standard curve;

[0035] Figure 3 This is a graph showing TFR expression in WB experiments and its internal reference;

[0036] Figure 4 is a graph showing PTGS2 expression in WB experiments and its internal reference;

[0037] Figure 5 is a graph showing GPX4 expression in WB experiments and its internal reference;

[0038] Figure 6 is a TTC staining graph of the cerebral infarction volume ratio of the sham operation group, model group, Example 1 and Comparative Examples 1-3;

[0039] Figure 7 is a TTC staining graph of the cerebral infarction volume ratio in the sham operation group, model group, Example 6 and Comparative Example 4;

[0040] Figure 8 This is a picture of HE staining of brain tissue of a rat in the sham operation group;

[0041] Figure 9 This is a picture of HE staining of brain tissue of a rat in the model group;

[0042] Figure 10 This is a HE staining image of a rat brain tissue in Example 1;

[0043] Figure 11 This is a picture of HE staining of a rat brain tissue in Example 6;

[0044] Figure 12 This is a picture of HE staining of a rat brain tissue in Comparative Example 1;

[0045] Figure 13 is a graph of HE staining of a certain rat brain tissue in Comparative Example 2;

[0046] Figure 14 is a graph of HE staining of a certain rat brain tissue in Comparative Example 3;

[0047] Figure 15 is a graph of HE staining of a certain rat brain tissue in Comparative Example 4. DETAILED DESCRIPTION

[0048] The technical solutions in the embodiments of the present application are described clearly and completely, obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the present application.

[0049] Embodiment 1

[0050] A Wulian Yinaotong for relieving ischemic stroke is prepared from 15g of Euonymus fortunei, 15g of Ficus tikoua, 15g of Spatholobus suberectus, 10g of Millettia pinnata, 15g of Tetrastigma obtectum, 10g of Asparagus cochinchinensis, 15g of Euryale ferox and 9g of Panax notoginseng by weight.

[0051] A preparation method of a Wulian Yinaotong for relieving ischemic stroke, the preparation method comprising:

[0052] S1, cut Spatholobus suberectus, Euonymus fortunei, Ficus tikoua, Millettia pinnata and Tetrastigma obtectum into small pieces, grind Panax notoginseng into powder, and weigh the medicinal materials;

[0053] S2, soak Euonymus fortunei, Ficus tikoua, Spatholobus suberectus, Millettia pinnata, Tetrastigma obtectum and Asparagus cochinchinensis in 800mL of water for 40min, boil with a large fire, slow-cook with a small fire for 20min, add Euryale ferox and Panax notoginseng powder, slow-cook with a small fire for 15min, filter, take the medicinal liquid, and prepare the Wulian Yinaotong.

[0054] Embodiment 2

[0055] A Wulian Yinaotong for relieving ischemic stroke is prepared from 17g of Euonymus fortunei, 17g of Ficus tikoua, 17g of Spatholobus suberectus, 12g of Millettia pinnata, 17g of Tetrastigma obtectum, 12g of Asparagus cochinchinensis, 17g of Euryale ferox and 10g of Panax notoginseng by weight.

[0056] A preparation method of a Wulian Yinaotong for relieving ischemic stroke, the preparation method comprising:

[0057] S1, cut Spatholobus suberectus, Euonymus fortunei, Ficus tikoua, Millettia pinnata and Tetrastigma obtectum into small pieces, grind Panax notoginseng into powder, and weigh the medicinal materials;

[0058] S2, Euonymus, Ficus, Sargentodoxa, Millettia, Smilax and Asparagus are soaked with 800ml water for 40 minutes, boiled with big fire, slowly boiled for 20 minutes with small fire, add Spathoglottis and Panax powder, slowly boiled for 20 minutes with small fire, filter, take medicine liquid, and five lotus brain benefit is prepared.

[0059] Example 3

[0060] A five lotus brain benefit for relieving ischemic stroke is prepared from 13g Euonymus, 13g Ficus, 13g Sargentodoxa, 8g Millettia, 13g Smilax, 8g Asparagus, 13g Spathoglottis and 8g Panax.

[0061] A preparation method of a five lotus brain benefit for relieving ischemic group, the preparation method comprises:

[0062] S1, Sargentodoxa, Euonymus, Ficus, Millettia and Smilax are cut into small pieces, and Panax is ground into powder, and the medicine materials are weighed;

[0063] S2, Euonymus, Ficus, Sargentodoxa, Millettia, Smilax and Asparagus are soaked with 500ml water for 30 minutes, boiled with big fire, slowly boiled for 15 minutes with small fire, add Spathoglottis and Panax powder, slowly boiled for 15 minutes with small fire, filter, take medicine liquid, and five lotus brain benefit is prepared.

[0064] Example 4

[0065] A five lotus brain benefit for relieving ischemic stroke is prepared from 20g Euonymus, 20g Ficus, 20g Sargentodoxa, 15g Millettia, 20g Smilax, 15g Asparagus, 20g Spathoglottis and 15g Panax.

[0066] A preparation method of a five lotus brain benefit for relieving ischemic group, the preparation method comprises:

[0067] S1, Sargentodoxa, Euonymus, Ficus, Millettia and Smilax are cut into small pieces, and Panax is ground into powder, and the medicine materials are weighed;

[0068] S2, Euonymus, Ficus, Sargentodoxa, Millettia, Smilax and Asparagus are soaked with 800ml water for 40 minutes, boiled with big fire, slowly boiled for 20 minutes with small fire, add Spathoglottis and Panax powder, slowly boiled for 20 minutes with small fire, filter, take medicine liquid, and five lotus brain benefit is prepared.

[0069] Example 5

[0070] A Wulian Yinaotong for alleviating ischemic stroke, wherein the raw materials of the Wulian Yinaotong are made from 10g of euonymus fortunei, 10g of quinquefolius quinquefolius, 10g of millettia reticulata, 5g of oxalis, 10g of scutellaria baicalensis, 5g of zouma tai, 10g of chrysanthemum indica and 5g of panax notoginseng, calculated by weight.

[0071] A preparation method for alleviating ischemic group Wulian Yinaotong, the preparation method comprising:

[0072] S1, cut Millettia spatholobi, Euonymus fortunei, Trichosanthes kirilowii, Cynanchum indica, and Cynanchum chinense into small pieces, grind Panax notoginseng into powder, and weigh the medicinal materials;

[0073] S2. Soak Euonymus fortunei, Psoralea corylifolia, Millettia repens, Cynanchum wilfordii, Cynanchum lappa and Trametes truncatum in 500 mL of water for 20 min, boil over high heat, simmer over low heat for 10 min, add Polygonum multiflorum and Panax notoginseng powder, simmer over low heat for 10 min, filter, and take the medicinal liquid to prepare Wulian Yinaotong.

[0074] Example 6

[0075] A Wulian Yinaotong for relieving ischemic stroke. The raw materials of the Wulian Yinaotong are made from 15g of Euonymus fortunei, 15g of Trichosanthes kirilowii, 15g of Millettia reticulata, 10g of Diplocaculata strychnifolia, 15g of Cynanchum wilfordii, 10g of Trametes dasyphylla, 15g of Cynanchum rotundum, 10g of Terrestris truncatum, 15g of Polygonum multiflorum, 9g of Panax notoginseng, 10g of Earthworm and 10g of Prunella vulgaris.

[0076] A preparation method for alleviating ischemic group Wulian Yinaotong, the preparation method comprising:

[0077] S1, cut Millettia spatholobi, Euonymus fortunei, Trichosanthes kirilowii, Cynanchum indica, and Cynanchum chinense into small pieces, grind Panax notoginseng into powder, and weigh the medicinal materials;

[0078] S2. Soak Euonymus fortunei, Psoralea corylifolia, Millettia repens, Cynanchum grandiflorum, Cynanchum lappa, Psoralea corylifolia, Psoralea corylifolia, Psoralea corylifolia, and Prunella vulgaris in 600 mL of water for 20 min, boil over high heat, and simmer over low heat for 10 min. Add Polygonum multiflorum and Panax notoginseng powder, simmer over low heat for 10 min, filter, and take the medicinal liquid to prepare Wulian Yinaotong.

[0079] Comparative Example 1

[0080] It is basically the same as Example 1, except that the components are different.

[0081] A Wulian Yinaotong for alleviating ischemic stroke. The raw materials of the Wulian Yinaotong are made from 15g of euonymus fortunei, 15g of quinquefolius quinquefolius, 15g of calamus calamus, 10g of oxalis, 15g of clematis chinensis, 10g of scutellaria baicalensis, 10g of terrestris, 15g of water lily and 9g of Panax notoginseng, calculated by weight.

[0082] Comparative Example 2

[0083] It is basically the same as Example 1, except that the components are different.

[0084] A Wulian Yinaotong for alleviating ischemic stroke, wherein the raw materials of the Wulian Yinaotong are made from 15g of Euonymus fortunei, 15g of Prunus mume, 15g of Acorus calamus, 10g of Radix Codonopsis pilosulae, 10g of Radix Eupatorii, 15g of Radix Eupatorii var. xanthophylla and 9g of Radix notoginseng by weight.

[0085] Comparative Example 3

[0086] It is basically the same as Example 1, except that the components are different.

[0087] A Wulian Yinaotong for alleviating ischemic stroke. The raw materials of the Wulian Yinaotong are made from 15g of Euonymus fortunei, 15g of Prunus mume, 10g of Trametes zoell, 15g of Polygonum multiflorum and 9g of Panax notoginseng by weight.

[0088] Comparative Example 4

[0089] It is basically the same as Example 6, except that the components are different.

[0090] A Wulian Yinaotong for alleviating ischemic stroke. The raw materials of the Wulian Yinaotong are prepared by weight from 15g of Euonymus fortunei, 15g of Millettia reticulata, 10g of Diplococtanax grandiflora, 10g of Trametes zoumata, 15g of Polygonum multiflorum, 9g of Panax notoginseng and 10g of earthworm.

[0091] 1. Experimental subjects:

[0092] The experimental animals were SPF male SD rats, 6-8 weeks old, weighing 180-220 g, provided by the laboratory of Guangxi University of Chinese Medicine.

[0093] 2. Build the model:

[0094] SD rats were subjected to middle cerebral artery occlusion to simulate ischemic stroke, specifically: a longitudinal incision was made in the middle of the neck of the rats, the skin and fascia were separated, the deep neck membrane was torn open, and blunt separation was performed downward along the left edge of the sternocleidomastoid muscle to expose the left common carotid artery. The left common carotid artery, the internal carotid artery and the external carotid artery were gently separated. A small opening was cut on the external carotid artery, and the prepared embolism line was inserted from the external neck incision, turned into the internal carotid artery, and the line plug was slowly pushed into the middle cerebral artery at an angle of 45° upward to block the middle cerebral artery opening. After two hours of embolism, the embolism line head was gently withdrawn to the common carotid artery, and the excess embolism line was cut off to cause ischemia-reperfusion injury. The model group and the sham operation group were gavaged with normal saline, and Example 1, Example 6 and Comparative Examples 1-4 were gavaged with the corresponding Wulian Yinaotong.

[0095] 3. The experimental rats were subjected to behavioral assessment (as shown in Tables 1 and 2). The assessment results were used to evaluate the rats' motor ability. The behavioral assessment used the Zea-Longa scoring method to evaluate the degree of neurological impairment in the rats. The Zea-Longa scoring criteria are as follows:

[0096] 0 points: no neurological deficit;

[0097] 1 point: the front paw on the paralyzed side cannot be fully extended and retracts inward when the tail is lifted;

[0098] 2 points: turning to the opposite side during spontaneous walking;

[0099] 3 points: leaning to the opposite side during spontaneous walking and being unable to stand;

[0100] 4 points means the patient cannot walk spontaneously and has loss of consciousness.

[0101] Table 1 Behavioral scores of the model group, sham operation group, Example 1 and Example 6

[0102]

[0103] Table 2 Behavioral scores of comparative examples 1-4

[0104]

[0105] The results are shown in Tables 1 and 2. The sham-operated group had the lowest score of 0 in each period, the model group had the highest behavioral score, and the behavioral scores of Example 1, Example 6, and Comparative Examples 1-4 were lower than those of the model group, indicating that the behavioral scores of the rats in the model group would increase if no drug was administered, indicating that the agent of the present application can effectively reduce the behavioral scores of the model rats to a certain extent.

[0106] 4. The concentrations of ferrous ion solutions used to draw the ferrous ion standard curve were 0 ug / mL, 2 ug / mL, 4 ug / mL, 6 ug / mL, 8 ug / mL, and 10 ug / mL. The OD values ​​of the ferrous ions were determined by colorimetry. The results are shown in Table 3.

[0107] Table 3 OD values ​​for drawing the standard curve of ferrous ion

[0108]

[0109] Draw a standard curve based on the above solution concentrations and the corresponding mean values. Draw the obtained standard curve as shown in the figure. Figure 1 As shown, the standard curve equation of ferrous ion is Y=15.521x-0.6729, R 2 =0.9991.

[0110] The OD values ​​of the sham operation group, model group, Example 1, Example 6, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 were respectively measured by colorimetry, the OD mean was calculated, and the OD mean was substituted into the formula Y = 15.521x-0.6729 to obtain the corresponding concentration. The results are shown in Table 4.

[0111] Table 4 Mean OD values ​​and ferrous ion concentrations of experimental rats in each group

[0112] OD mean Concentration (ug / mL) Concentration (ug / g) Sham operation group 0.186 2.212 22.12 Model Group 0.247 3.159 31.59 Example 1 0.210 2.585 25.85 Example 6 0.195 2.354 23.54 Comparative Example 1 0.220 2.742 27.42 Comparative Example 2 0.227 2.850 28.50 Comparative Example 3 0.240 3.052 30.52 Comparative Example 4 0.226 2.835 28.35

[0113] As can be seen from Table 4, the ferrous ion concentration of the model group was 31.59 ug / g, which was much higher than the ferrous ion concentration of the sham operation. After administration, the ferrous ion concentrations of the ischemic stroke model rats of Example 1, Example 6, and Comparative Examples 1-4 decreased to varying degrees relative to the rats in the model group, with the ferrous ion concentrations of Examples 1 and 6 decreasing the most. This indicates that the administration of the Wulian Yinaotong decoction of the present application has a significant therapeutic effect on reducing ferrous ions in the blood of rats with ischemic stroke.

[0114] 5. The concentrations of the glutathione solution gradient used to draw the glutathione standard curve were 0 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, and 300 μg / mL. The OD values ​​of the glutathione were determined by colorimetry. The results are shown in Table 5.

[0115] Table 5 Data used to draw the glutathione standard curve

[0116]

[0117] Draw a standard curve based on the above solution concentrations and the corresponding mean values. Draw the obtained standard curve as shown in the figure. Figure 2 As shown, the glutathione standard curve equation is Y = 424.44x-46.241, R 2 =0.9996.

[0118] The glutathione OD values ​​of the sham operation group, model group, Example 1, Example 6, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 were respectively measured by colorimetry, the OD mean was calculated, and the OD mean was substituted into the formula Y = 424.44x-46.241 to obtain the corresponding concentration. The results are shown in Table 6.

[0119] Table 6 OD mean and glutathione content of experimental rats in each group

[0120]

[0121]

[0122] It can be concluded from Table 6 that the glutathione content of rats in the model group is relatively low. The low glutathione content generally reflects the reduced detoxification ability or antioxidant capacity of the liver in the body. The use of Wulian Yinaotong in Example 1 and Example 6 can increase the glutathione content to a level close to that in the sham operation group, indicating that the Wulian Yinaotong of the present application has the ability to improve the detoxification ability of the liver or enhance the antioxidant capacity of the body.

[0123] 6. The brains of three rats with ischemic stroke model in each group were taken to measure their dry weight and wet weight, and the brain water content (%) was calculated. The mean dry weight, mean wet weight and water content are shown in Table 7. The water content was calculated as follows: Water content (%) = (wet weight - dry weight) / dry weight.

[0124] Table 7 Brain water content in each group

[0125] Average dry weight (g) Average wet weight (g) Water content (%) Sham operation group 0.505 1.796 71.89 Model Group 0.338 1.940 82.59 Example 1 0.470 1.847 74.57 Example 6 0.484 1.827 73.52 Comparative Example 1 0.435 1.869 76.70 Comparative Example 2 0.424 1.879 77.43 Comparative Example 3 0.348 1.895 81.64 Comparative Example 4 0.465 1.870 75.15

[0126] As shown in Table 7, ischemic stroke causes local edema, leading to increased brain wet weight and increased brain water content. While the brain wet weight of rats with ischemic stroke receiving Wulian Yinaotong remained higher than that of the sham-operated group, it was significantly lower than that of the model group. This suggests that Wulian Yinaotong can alleviate local edema caused by ischemic stroke and reduce the extent of brain nerve and tissue damage or death caused by local edema.

[0127] 6. The expression of TFR (transferrin receptor), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), PTGS2 (cyclooxygenase 2), and GPX4 (glutathione peroxidase 4) in the sham operation group, model group, and Example 1 was detected. The expression of GAPDH was analyzed twice, and the expression results are shown in Tables 8 and 9. The expression of TFR is shown in Table 9. Figure 3 As shown, PTGS2 expression Figure 4 As shown, GPX4 expression Figure 5 shown.

[0128] Table 8 TFR, GAPDH, and PTGS2 expression in the sham group and Example 1

[0129]

[0130] Table 9 TFR, GAPDH, and PTGS2 expression in the model group and Example 1

[0131]

[0132]

[0133] 7. The cerebral infarction volume of the model rats in the model group and the Example 1 group was measured by TTC staining, and photos of the brain tissue infarction foci after staining were taken. Normal tissue was red, and the infarcted area was grayish white. Figure 6 and Figure 7 ) Image-Pro Plus 6.0 software was used for analysis and processing, and the percentage of brain tissue infarct volume in the whole brain was calculated. The results are shown in Table 10.

[0134] Table 10 Ratio of cerebral infarction area and cerebral infarction volume in rats in the model group and Example 1

[0135]

[0136] It can be concluded from Table 0 that the Wulian Yinaotong of Example 1 using the method of the present invention significantly reduces the proportion of cerebral infarction. Comparison of Example 1 with Comparative Example 1 shows that the effect of using Millettia repens in Wulian Yinaotong on reducing cerebral infarction is better than that of using Acorus calamus.

[0137] 8. HE staining was used to observe the pathological changes of brain tissue in experimental rats. The specific steps were: fixation with paraformaldehyde, gradient dehydration, paraffin embedding, sectioning, baking, hematoxylin-eosin (HE) staining, and sealing with gum to observe the pathological changes of brain tissue. The results were as follows: Figure 8-15 shown.

[0138] Sham operation group ( Figure 8 ),from Figure 8 It can be seen that the brain tissue structure is normal, neurons are evenly distributed, cell outlines are clear, and no obvious degeneration is observed; Figure 9 The number of neurons in the cerebral infarction area of ​​the model group decreased, the remaining neurons were necrotic and degenerated, the cell nuclei were condensed and stained dark blue-black, the cell bodies became smaller, the surrounding tissues were loose and edematous, and the gaps were enlarged. Example 1 ( Figure 10 ) and Example 6 ( Figure 11 ) had a normal brain tissue structure and clearer cell outlines than the model group. Figure 12 ), Comparative Example 2 ( Figure 13 ), Comparative Example 3 ( Figure 14 ) and Comparative Example 4 ( Figure 15 ) cell bodies were also better than the model group, but the effect was not as good as that of Example 1 and Example 6.

[0139] The present invention can be implemented in various ways and is not limited to the embodiments described above. A person skilled in the art will appreciate that the present invention can be implemented in other specific ways without changing the technical concept or essential features of the present invention. Therefore, it should be understood that the embodiments described above are illustrative and not intended to limit the present invention.

Claims

1. A Wulian Yinaotong for alleviating ischemic stroke, characterized in that: By weight, the raw materials of the Wulian Yinaotong are composed of 10-20g of Fufang Caulis, 10-20g of Wuzhimao Peach, 10-20g of Millettia reticulata, 5-15g of Niu Dali, 10-20g of Kuanjin Caulis, 5-15g of Zoumatai, 10-20g of Huanghua Daoshuilian and 5-15g of Panax notoginseng.

2. The Wulian Yinaotong for alleviating ischemic stroke according to claim 1, characterized in that: By weight, the raw materials of the Wulian Yinaotong are composed of 13-17g of Fufang Caulis, 13-17g of Wuzhimao Peach, 13-17g of Millettia reticulata, 8-12g of Niu Dali, 13-17g of Kuanjin Caulis, 8-12g of Zoumatai, 13-17g of Huanghua Daoshuilian and 8-10g of Panax notoginseng.

3. The Wulian Yinaotong for alleviating ischemic stroke according to claim 2, characterized in that: By weight, the raw materials of the Wulian Yinaotong are composed of 15g of Fufang Caulis, 15g of Wuzhimao Peach, 15g of Millettia reticulata, 10g of Niu Dali, 15g of Kuanjin Caulis, 10g of Zoumatai, 15g of Huanghuadaoshuilian and 9g of Panax notoginseng.

4. A Wulian Yinaotong for alleviating ischemic stroke, characterized in that: By weight, the raw materials of the Wulian Yinaotong are composed of 10-20g of Euonymus fortunei, 10-20g of Prunella vulgaris, 10-20g of Millettia reticulata, 5-15g of Diplococephala, 10-20g of Caulis Achyranthis Bidentatae, 5-15g of Terrestris strychnifolia, 10-20g of Herba Lycopodii flavescentis, 5-15g of Radix Psoraleae, 5-15g of Radix Notoginseng, 5-15g of Pheretima chinensis and 5-15g of Herba Prunellae.

5. The Wulian Yinaotong for alleviating ischemic stroke according to claim 4, characterized in that: By weight, the raw materials of the Wulian Yinaotong are composed of 15g of Fufang Caulis, 15g of Wuzhimi Cai, 15g of Millettia reticulata, 10g of Niu Dali, 15g of Kuanjin Caulis, 10g of Zoumatai, 15g of Huanghua Daoshui Lian, 9g of Panax notoginseng, 10g of earthworm and 10g of Prunella vulgaris.

6. The Wulian Yinaotong for alleviating ischemic stroke according to any one of claims 1 to 5, characterized in that: The Wulian Yinaotong is an internal medicine, and the method of taking it is to take it warm once in the morning, noon and evening.

7. The method for preparing Wulian Yinaotong for alleviating ischemic stroke according to any one of claims 1 to 3, characterized in that: The preparation method comprises: S1, cut Millettia spatholobi, Euonymus fortunei, Trichosanthes kirilowii, Cynanchum indica, and Cynanchum chinense into small pieces, grind Panax notoginseng into powder, and weigh the medicinal materials; S2. Soak Euonymus fortunei, Eleutherococcus quinquefolius, Millettia repens, Cynanchum wilfordii, Cynanchum lappa, and Trametes zognathifolia in 500-800 mL of water for 20-40 min, boil over high heat, and simmer over low heat for 10-20 min. Add Rhizoma Coptidis and Panax notoginseng powder, simmer over low heat for 10-20 min, filter, and obtain the medicinal liquid to prepare Wulian Yinaotong.

8. The method for preparing Wulian Yinaotong for alleviating ischemic stroke according to any one of claims 4-5, characterized in that: The preparation method comprises: S1, cut Millettia spatholobi, Euonymus fortunei, Trichosanthes kirilowii, Cynanchum indica, and Cynanchum chinense into small pieces, grind Panax notoginseng into powder, and weigh the medicinal materials; S2. Soak Euonymus fortunei, Psoralea corylifolia, Millettia reticulata, Cynanchum wilfordii, Cynanchum chinense, Psoralea corylifolia, Psoralea corylifolia, Psoralea corylifolia, and Prunella vulgaris in 500-800 mL of water for 20-40 min, boil over high heat, and simmer over low heat for 10-20 min. Add Polygonum multiflorum and Panax notoginseng powder, simmer over low heat for 10-20 min, filter, and take the medicinal liquid to prepare Wulian Yinaotong.

Images