Strain culture equipment for wine brewing

Abstract

The invention discloses strain culture equipment for wine brewing, and relates to the technical field of strain reproduction. The device comprises an oxygen supply assembly, the oxygen supply assembly is fixedly connected with a shell, the outer side face of a branch pipe is fixedly connected with the inner side face of the oxygen supply assembly, an inner cavity of the branch pipe communicates with an oxygen supply pipeline of the oxygen supply assembly, the end, away from a bottom plate, of the branch pipe extends into the shell, and the outer side face of the branch pipe is fixedly connected with the inner side face of the shell; an inner cavity of the branch pipe is communicated with an inner cavity of the shell, the outer side face of the exhaust assembly is fixedly connected with the inner side face of the shell, the oxygen supply assembly pumps oxygen into the shell, in order to ensure that the pressure in the strain breeding environment is constant, the exhaust assembly exhausts redundant carbon dioxide, and it is ensured that strains are always in the proper oxygen concentration; the method ensures aerobic respiration of the strain, thoroughly decomposes nutrient substances, makes full use of nutritional ingredients such as saccharides in grape juice, and meets a large amount of energy demands in the growth and reproduction process of the strain.

Description

Technical Field

[0001] The invention relates to the technical field of bacterial strain propagation, and in particular to bacterial strain culture equipment for wine brewing. Background Art

[0002] The commonly used strain of bacteria in winemaking is brewer's yeast, and its cultivation process is roughly as follows: first obtain a standard brewer's yeast strain from a professional preservation institution or separate and screen yeast with good performance from high-quality wine, then select a suitable culture medium, which generally contains nutrients such as sugars, nitrogen sources, and inorganic salts, adjust the appropriate pH value and temperature, and first activate and culture it in an aerobic environment to allow yeast cells to recover and grow quickly. When the number of yeast reaches a certain scale, it can be transferred and expanded as needed. During the cultivation process, attention should be paid to prevent contamination by foreign bacteria. The purity of yeast culture should be ensured through aseptic operation and the use of appropriate disinfection and sterilization equipment. The cultured yeast can be used in the fermentation stage of winemaking, and it is inoculated into grape juice to start fermentation and convert the sugars in the grape juice into alcohol and other products;

[0003] Compared with winemaking, bacterial reproduction needs to be carried out in an aerobic environment all the time to ensure that aerobic respiration consumes nutrients smoothly and ensures the rapid reproduction of bacteria. Therefore, it is necessary to control the oxygen content in the reproduction environment. At the same time, temperature and pressure are also key factors to ensure the rapid reproduction of bacteria. Therefore, we proposed a bacterial culture equipment for winemaking. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a bacterial strain culture device for wine brewing, comprising:

[0005] A bottom plate, a shell is arranged on the top of the bottom plate;

[0006] A culture mechanism, wherein the culture mechanism is fixedly connected to the top of the bottom plate, the top of the culture mechanism is fixedly connected to the bottom of the shell, and a part of the culture mechanism is fixed to the inner side of the shell;

[0007] Wherein, the training institution includes:

[0008] A top cover, wherein the top cover is arranged on a side of the shell away from the bottom plate, and the inner side surface of the top cover is threadedly connected to the outer side surface of the shell, and two feeding pipes are symmetrically arranged on the top of the top cover, and one end of the two feeding pipes close to the shell both penetrates the top cover, and the inner side surface of the top cover is fixedly connected to the outer surface of the feeding pipe, and the two feeding pipes are controlled by valves, and are used for feeding nutrients and mother seeds respectively;

[0009] An oxygen supply component, which has a heating structure and is used to provide suitable temperature and oxygen for bacterial reproduction. The oxygen supply component is fixedly connected to the top of the bottom plate, and the side of the oxygen supply component away from the bottom plate is fixedly connected to the side of the shell close to the bottom plate;

[0010] A branch pipe, wherein the branch pipe is arranged outside the shell, and one end of the branch pipe close to the bottom plate extends into the interior of the oxygen supply assembly, the outer side surface of the branch pipe is fixedly connected to the inner side surface of the oxygen supply assembly, and the inner cavity of the branch pipe is communicated with the oxygen supply pipeline of the oxygen supply assembly, and one end of the branch pipe away from the bottom plate extends into the interior of the shell, the outer side surface of the branch pipe is fixedly connected to the inner side surface of the shell, and the inner cavity of the branch pipe is communicated with the inner cavity of the shell;

[0011] An exhaust component, the exhaust component having an opening and closing structure, is used to discharge carbon dioxide generated by bacterial growth, and the outer side surface of the exhaust component is fixedly connected to the inner side surface of the shell;

[0012] Grape juice and activated mother bacteria solution are added to the shell from two feeding pipes respectively, and the oxygen supply component is started. The oxygen supply component pumps external oxygen into the shell to provide oxygen for the reproduction of the mother bacteria. At the same time, the heating structure of the oxygen supply component heats the gas to provide a suitable temperature environment for the reproduction of the mother bacteria. The gas in the oxygen supply pipeline of the oxygen supply component flows, the pressure is reduced, and the gas inside the branch pipe is sucked into the oxygen supply component, so that the other end of the branch pipe draws gas from the upper part of the shell. Since the density of oxygen is lower than that of carbon dioxide, the branch pipe will draw the oxygen at the top of the shell into the bacterial solution for bacterial reproduction, which can make full use of the inside of the shell. Oxygen. During reproduction, the amount of oxygen consumed by the bacteria is always the same as the amount of carbon dioxide. At the same time, the oxygen supply component pumps oxygen into the shell. In order to ensure the constant pressure in the bacterial reproduction environment, it is necessary to discharge excess carbon dioxide. The exhaust component is started to discharge excess carbon dioxide to ensure that the bacteria are always in an appropriate oxygen concentration, ensure that the bacteria can carry out aerobic respiration, thoroughly decompose nutrients, and make full use of nutrients such as sugars in the grape juice to meet the large demand for energy during the growth and reproduction of the bacteria, and support cells to carry out various life activities, such as protein synthesis, DNA replication, etc., thereby promoting the rapid reproduction of the bacteria.

[0013] Furthermore, one end of the branch pipe away from the bottom plate penetrates through one-half of the outer shell and is slightly above it, the exhaust component is arranged at a position slightly below the position where the outer shell is penetrated by the branch pipe, the branch pipe is located in the upper half of the remaining space after the bacterial solution is added to the outer shell, and the exhaust component is arranged in the lower half, which can ensure that the branch pipe inhales a large amount of oxygen and that the exhaust component fully discharges carbon dioxide, thereby maintaining the bacterial reproduction environment, that is, the aerobic environment inside the outer shell, avoiding anaerobic respiration of the bacterial strain, and ensuring the utilization efficiency of the grape juice.

[0014] Furthermore, one end of the branch pipe located inside the shell is fixedly connected with a ring tube, the ring tube is embedded in the shell, and the outer side surface of the ring tube is fixedly connected to the inner side surface of the shell, the ring tube is hollow, and the inner cavity of the ring tube is connected to the inner cavity of the shell and the inner cavity of the branch pipe, and the outer side surface of the ring tube close to the center of the shell is made of hydrophobic material, and the gas above the shell is sucked into the ring tube and finally enters the branch pipe. Since the inner circle of the outer side surface of the ring tube is made of hydrophobic material, only gas passes into the interior of the ring tube, thereby avoiding the loss of bacteria when dumping.

[0015] Furthermore, the oxygen supply assembly includes a pump body, one end of the pump body away from the center of the bottom plate is fixedly connected to an air intake pipe, one end of the pump body away from the air intake pipe is fixedly connected to an oxygen tube, and one end of the oxygen tube away from the pump body is located directly below the outer shell. When the pump body is started, the pump body drives external oxygen to pass through the air intake pipe, the pump body, and the oxygen tube in sequence, and finally enters the interior of the outer shell.

[0016] Furthermore, a cylinder is provided on the outside of the air inlet pipe, and the cylinder is made of a heat-insulating material, and the cylinder is fixedly connected to the top of the base plate, and a side of the cylinder away from the base plate is fixedly connected to a side of the outer shell close to the base plate, an end of the pump body close to the oxygen tube is fixedly connected to the outer side surface of the cylinder, and an end of the oxygen tube close to the pump body passes through the cylinder, and the outer side surface of the oxygen tube is fixedly connected to the inner side surface of the cylinder, the branch pipe extends into the interior of the cylinder, and an end of the branch pipe close to the base plate is fixedly connected to the outer side surface of the oxygen tube, and the inner cavity of the branch pipe is communicated with the inner cavity of the oxygen tube, and the cylinder is used to protect the oxygen tube, and when heated, the cylinder made of heat-insulating material maintains the temperature inside the oxygen tube.

[0017] Furthermore, the oxygen tube is bent into a spiral shape inside the cylinder, and a heating wire is sheathed on the outside of the oxygen tube, and the heating wire is arranged in a spiral shape. The end surface of the heating wire is fixedly connected to the bottom of the inner side surface of the cylinder. The heating wire is started and generates heat, thereby heating the oxygen passing through the oxygen tube to a suitable temperature. The spiral oxygen tube can be provided with a longer path inside the cylinder, and with the heating wire sheathed on the outside, the oxygen can be heated for a longer time, ensuring that the oxygen entering the inside of the outer shell is fully heated, and the heating wire helically wound around the outside of the oxygen tube can heat the oxygen more evenly and obtain a more accurate culture temperature of the bacteria.

[0018] Furthermore, a filter is fixedly connected to one end of the air inlet pipe away from the pump body, and the filter is used to filter impurities, microorganisms and other harmful substances in the air. The outer side of the filter is fixedly connected to a support plate, and the side of the support plate away from the filter is fixedly connected to the top of the bottom plate. The provision of the filter can increase the purity of the air entering the interior of the outer casing and prevent impurities, microorganisms and other harmful substances in the air from affecting the reproduction of bacteria.

[0019] Furthermore, a circular plate is fixedly connected to the inner side surface of the cylinder, and the circular plate is arranged on the side of the oxygen tube away from the bottom plate, the end of the electric heating wire away from the bottom plate is fixedly connected to the side of the circular plate close to the bottom plate, a plurality of holes are evenly opened on the surface of the circular plate, and the hole walls of the circular plate are fixedly connected to the gas injection pipe, and the end of the gas injection pipe away from the circular plate is bent toward the side close to the circular plate, and the gas injection pipe is arranged in a U-shape, and an air stone is arranged inside the vertical pipe of the gas injection pipe away from the circular plate, and the outer side surface of the air stone is fixedly connected to the inner side surface of the gas injection pipe, and the air inside the oxygen tube is injected into the cylinder, and then enters the gas injection pipe, and finally passes through the air stone. After entering the interior of the shell, the setting of the air stone can block the air injection tube to prevent the culture solution from entering the air injection tube, and the air injection tube is set to an inverted U-shape, with one end located inside the solution. Due to the pressure and the continuous introduction of gas at the other end, it is difficult for the culture solution to enter the air injection tube. The loose porous structure of the air stone can produce a large number of bubbles when the gas is introduced. The bubbles can drive the solution to roll, so that the oxygen and heat in the gas are fully mixed with the culture solution, and the tumbling solution, the nutrients therein and the bacteria can be fully mixed to achieve an effect similar to stirring. While providing oxygen for the reproduction of the bacteria, the present invention does not need to set a stirring structure of traditional culture equipment.

[0020] Furthermore, the exhaust assembly includes an exhaust pipe, which is arranged on the outside of the shell, and one end of the exhaust pipe close to the shell extends to the inside of the shell, and the outer side surface of the exhaust pipe is fixedly connected to the inner side surface of the shell, and the exhaust pipe is controlled by a valve. Due to the injection of air, the pressure inside the shell rises, and then the air inside the shell is finally pressed into the exhaust pipe. Since the exhaust pipe is arranged at the lower part of the remaining inner cavity after the shell is filled with grape juice, the carbon dioxide with a higher density will be preferentially discharged from the outside of the shell.

[0021] Furthermore, an exhaust pipe is fixedly connected to the inner side surface of the outer shell, the exhaust pipe is hollow and made of elastic material, a tube body is arranged inside the exhaust pipe, one end of the tube body close to the center of the outer shell passes through the exhaust pipe, and the outer side surface of the tube body is fixedly connected to the inner side surface of the exhaust pipe, a plurality of tube bodies are evenly distributed along the circumference of the exhaust pipe, one end of the tube body located outside the exhaust pipe is fixedly connected to a film, and the film is made of hydrophobic material, a fixed sleeve is arranged inside the exhaust pipe, the outer side surface of the fixed sleeve is fixedly connected to the inner side surface of the exhaust pipe, and the inner side surface of the fixed sleeve is fixedly connected to the outer side surface of the tube body, and the fixed sleeve is made of elastic material, air passes through the film and then enters The tube body enters the inner cavity of the exhaust pipe and finally enters the inside of the exhaust pipe and finally discharges from the outer shell to balance the pressure and discharge excess carbon dioxide. The film is made of hydrophobic material, which can prevent the solution from entering the inside of the tube body and avoid waste when dumping and unloading. Since the exhaust pipe is made of elastic material, when the outer shell is placed upright, the exhaust pipe is conical under the action of gravity, which makes it convenient to load materials from the feeding pipe. The raw materials enter the top of the circular plate. When unloading, the device is tipped and the device is turned upside down. Under the action of gravity, the exhaust pipe is conical in the other direction, which is convenient for concentrated unloading. When unloading, it is not easy to contaminate the remaining inner wall of the outer shell, reducing the loss of bacteria after breeding. Under the action of the mass of the tube body, etc., the exhaust pipe is counterweighted to make the conical structure more obvious and increase the smoothness of loading and unloading.

[0022] The present invention has the beneficial effects:

[0023] 1. The present invention sets a culture mechanism, and the oxygen supply component pumps external oxygen into the shell to provide oxygen for the reproduction of the mother bacteria. At the same time, the heating structure of the oxygen supply component heats the gas to provide a suitable temperature environment for the reproduction of the mother bacteria. The gas in the oxygen supply pipeline of the oxygen supply component flows, the pressure is reduced, and the gas in the branch pipe is sucked into the oxygen supply component, so that the other end of the branch pipe extracts gas from the upper part of the shell. Since the density of oxygen is lower than that of carbon dioxide, the branch pipe will draw the oxygen on the top of the shell into the bacterial solution for bacterial reproduction, which can make full use of the oxygen inside the shell. The amount of oxygen and carbon dioxide consumed by the bacteria is always the same. At the same time, the oxygen supply component pumps oxygen into the shell. In order to ensure the constant pressure in the bacterial breeding environment, excess carbon dioxide needs to be discharged. The exhaust component discharges excess carbon dioxide to ensure that the bacteria are always in an appropriate oxygen concentration, ensure that the bacteria can perform aerobic respiration, thoroughly decompose nutrients, and make full use of nutrients such as sugars in the grape juice to meet the large demand for energy during the growth and reproduction of the bacteria, and support cells to carry out various life activities, such as protein synthesis, DNA replication, etc., thereby promoting the rapid reproduction of the bacteria.

[0024] 2. The present invention provides an oxygen tube, a spiral oxygen tube, which can provide a longer path in the cylinder. Combined with the electric heating wire sheathed on the outside, the oxygen can be heated for a longer time, ensuring that the oxygen entering the shell is fully heated. The electric heating wire spirally wound on the outside of the oxygen tube can heat the oxygen more evenly and obtain a more accurate culture temperature of the bacteria.

[0025] 3. The present invention blocks the gas injection tube by setting an air stone to prevent the culture solution from entering the gas injection tube. The gas injection tube is set to an inverted U-shape with one end located inside the solution. Due to the pressure and the continuous introduction of gas at the other end, it is difficult for the culture solution to enter the gas injection tube. The loose porous structure of the air stone can generate a large number of bubbles when the gas is introduced. The bubbles can drive the solution to roll, so that the oxygen and heat in the gas are fully mixed with the culture solution. In the rolling solution, the nutrients and bacteria therein can be fully mixed to achieve an effect similar to stirring. While providing oxygen for the reproduction of the bacteria, the present invention does not need to set a stirring structure of traditional culture equipment.

[0026] 4. The present invention sets an exhaust component, and the film is made of hydrophobic material, which can prevent the solution from entering the inside of the tube body and avoid waste when dumping and unloading. Since the exhaust pipe is made of elastic material, when the shell is placed upright, the exhaust pipe is cone-shaped under the action of gravity, which can facilitate the raw materials to enter the top of the circular plate when loading from the loading pipe. When unloading, the device is dumped and the device is inverted. Under the action of gravity, the exhaust pipe is cone-shaped in the other direction, which is convenient for centralized unloading. When unloading, it is not easy to contaminate the remaining inner wall of the shell, thereby reducing the loss of bacteria after breeding. Under the action of the mass of the tube body, etc., the exhaust pipe is counterweighted to make the cone structure more obvious and increase the smoothness of loading and unloading. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a schematic diagram of a bacterial culture device for wine brewing according to the present invention;

[0028] Figure 2 It is a schematic diagram of the structure of the cultivation mechanism of the present invention;

[0029] Figure 3 It is a schematic diagram of the internal structure of the present invention;

[0030] Figure 4 It is a schematic diagram of the structure of the oxygen tube of the present invention;

[0031] Figure 5 This is a schematic diagram of the structure of the oxygen supply assembly of the present invention;

[0032] Figure 6 This is a schematic diagram of the cross-sectional structure of the gas injection pipe of the present invention;

[0033] Figure 7 It is a schematic diagram of the cross-sectional structure of the housing of the present invention;

[0034] Figure 8 It is a schematic diagram of the exhaust assembly structure of the present invention.

[0035] In the figure: 1, bottom plate; 2, outer shell; 3, culture mechanism; 31, top cover; 32, feeding pipe; 33, oxygen supply assembly; 331, pump body; 332, air inlet pipe; 333, oxygen pipe; 334, heating wire; 335, filter; 336, support plate; 337, round plate; 338, air injection pipe; 339, air stone; 3310, cylinder; 34, branch pipe; 35, ring cylinder; 36, exhaust assembly; 361, exhaust pipe; 362, exhaust cylinder; 363, tube body; 364, film; 365, fixing sleeve. DETAILED DESCRIPTION

[0036] The present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments. The embodiments of the present invention are provided for the purpose of illustration and description, and are not intended to be exhaustive or to limit the present invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention, and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for specific uses.

[0037] Example 1, please refer to Figure 1-Figure 6 The present invention is a bacterial strain culture device for wine brewing, comprising:

[0038] A bottom plate 1, with a housing 2 disposed on the top of the bottom plate 1;

[0039] The culture mechanism 3 is fixedly connected to the top of the bottom plate 1, the top of the culture mechanism 3 is fixedly connected to the bottom of the shell 2, and part of the culture mechanism 3 is fixed to the inner side of the shell 2;

[0040] Among them, training institution 3 includes:

[0041] A top cover 31, which is arranged on a side of the outer shell 2 away from the bottom plate 1, and the inner side of the top cover 31 is threadedly connected to the outer side of the outer shell 2. Two feeding pipes 32 are symmetrically arranged on the top of the top cover 31. One end of the two feeding pipes 32 close to the outer shell 2 passes through the top cover 31, and the inner side of the top cover 31 is fixedly connected to the outer surface of the feeding pipe 32. The two feeding pipes 32 are controlled by valves and are used for feeding nutrients and mother seeds respectively;

[0042] An oxygen supply component 33, which has a heating structure and is used to provide suitable temperature and oxygen for bacterial reproduction. The oxygen supply component 33 is fixedly connected to the top of the bottom plate 1, and a side of the oxygen supply component 33 away from the bottom plate 1 is fixedly connected to a side of the housing 2 close to the bottom plate 1;

[0043] The branch pipe 34 is arranged outside the shell 2, and one end of the branch pipe 34 close to the bottom plate 1 extends to the inside of the oxygen supply component 33, the outer side surface of the branch pipe 34 is fixedly connected to the inner side surface of the oxygen supply component 33, and the inner cavity of the branch pipe 34 is communicated with the oxygen supply pipeline of the oxygen supply component 33, and one end of the branch pipe 34 away from the bottom plate 1 extends to the inside of the shell 2, the outer side surface of the branch pipe 34 is fixedly connected to the inner side surface of the shell 2, and the inner cavity of the branch pipe 34 is communicated with the inner cavity of the shell 2;

[0044] An exhaust component 36, which has an opening and closing structure and is used to discharge carbon dioxide generated by bacterial growth. The outer side of the exhaust component 36 is fixedly connected to the inner side of the housing 2;

[0045] Grape juice and activated mother bacteria solution are added to the shell 2 from two feeding pipes 32, and the oxygen supply component 33 is started. The oxygen supply component 33 pumps external oxygen into the shell 2 to provide oxygen for the reproduction of the mother bacteria. At the same time, the heating structure of the oxygen supply component 33 heats the gas to provide a suitable temperature environment for the reproduction of the mother bacteria. The gas in the oxygen supply pipeline of the oxygen supply component 33 flows, and the pressure is reduced. The gas in the branch pipe 34 is sucked into the oxygen supply component 33, so that the other end of the branch pipe 34 extracts gas from the upper part of the shell 2. Since the density of oxygen is lower than that of carbon dioxide, the branch pipe 34 will draw the oxygen at the top of the shell 2 into the bacterial solution for bacterial reproduction, which can Make full use of the oxygen inside the shell 2. During reproduction, the amount of oxygen and carbon dioxide consumed by the bacteria is always the same. At the same time, the oxygen supply component 33 pumps oxygen into the shell 2. In order to ensure the constant pressure in the bacterial reproduction environment, it is necessary to discharge excess carbon dioxide. The exhaust component 36 is started to discharge excess carbon dioxide to ensure that the bacteria are always in an appropriate oxygen concentration, ensure that the bacteria can perform aerobic respiration, thoroughly decompose nutrients, and make full use of nutrients such as sugars in the grape juice to meet the large demand for energy during the growth and reproduction of the bacteria, support cells to carry out various life activities, such as protein synthesis, DNA replication, etc., thereby promoting the rapid reproduction of the bacteria.

[0046] One end of the branch pipe 34 away from the bottom plate 1 passes through one-half of the outer shell 2 and is slightly above it. The exhaust component 36 is arranged at a position slightly below the position where the outer shell 2 is penetrated by the branch pipe 34. The branch pipe 34 is located in the upper half of the remaining space after the bacterial solution is added to the outer shell 2, and the exhaust component 36 is arranged in the lower half. This can ensure that the branch pipe 34 inhales a large amount of oxygen and that the exhaust component 36 fully discharges carbon dioxide, thereby maintaining the bacterial reproduction environment, that is, the aerobic environment inside the outer shell 2, avoiding anaerobic respiration of the bacteria, and ensuring the utilization efficiency of the grape juice.

[0047] One end of the branch pipe 34 located inside the shell 2 is fixedly connected to a ring tube 35, the ring tube 35 is embedded in the shell 2, and the outer side of the ring tube 35 is fixedly connected to the inner side of the shell 2, the ring tube 35 is hollow, and the inner cavity of the ring tube 35 is connected to the inner cavity of the shell 2 and the inner cavity of the branch pipe 34, and the outer side of the ring tube 35 close to the center of the shell 2 is made of hydrophobic material. The gas above the shell 2 is sucked into the ring tube 35 and finally enters the branch pipe 34. Since the inner circle of the outer side of the ring tube 35 is made of hydrophobic material, only gas passes into the inside of the ring tube 35 to avoid the loss of bacteria when dumping.

[0048] The oxygen supply assembly 33 includes a pump body 331, one end of the pump body 331 away from the center of the bottom plate 1 is fixedly connected to an air intake pipe 332, and the other end of the pump body 331 away from the air intake pipe 332 is fixedly connected to an oxygen pipe 333, and the end of the oxygen pipe 333 away from the pump body 331 is located directly below the outer shell 2. When the pump body 331 is started, the pump body 331 drives the external oxygen to pass through the air intake pipe 332, the pump body 331, and the oxygen pipe 333 in sequence, and finally enters the interior of the outer shell 2.

[0049] A cylinder 3310 is provided on the outside of the air inlet pipe 332. The cylinder 3310 is made of a heat-insulating material and is fixedly connected to the top of the base plate 1. The side of the cylinder 3310 away from the base plate 1 is fixedly connected to the side of the shell 2 close to the base plate 1. One end of the pump body 331 close to the oxygen tube 333 is fixedly connected to the outer side of the cylinder 3310, and one end of the oxygen tube 333 close to the pump body 331 passes through the cylinder 3310, and the outer side of the oxygen tube 333 is fixedly connected to the inner side of the cylinder 3310. The branch pipe 34 extends into the inside of the cylinder 3310, and one end of the branch pipe 34 close to the base plate 1 is fixedly connected to the outer side of the oxygen tube 333, and the inner cavity of the branch pipe 34 is connected to the inner cavity of the oxygen tube 333. The cylinder 3310 is used to protect the oxygen tube 333. When heated, the cylinder 3310 made of heat-insulating material maintains the temperature inside the oxygen tube 333.

[0050] The oxygen tube 333 is bent into a spiral shape inside the cylinder 3310. The outer part of the oxygen tube 333 is sheathed with a heating wire 334, and the heating wire 334 is arranged in a spiral shape. The end surface of the heating wire 334 is fixedly connected to the bottom of the inner side surface of the cylinder 3310. The heating wire 334 is started and generates heat, thereby heating the oxygen passing through the oxygen tube 333 and heating it to a suitable temperature. The spiral oxygen tube 333 can be provided with a longer path in the cylinder 3310, and the heating wire 334 sheathed on the outer part thereof can heat the oxygen for a longer time, ensuring that the oxygen passing into the inner part of the outer shell 2 is fully heated. The heating wire 334 helically wound around the outer part of the oxygen tube 333 can heat the oxygen more evenly and obtain a more accurate culture temperature of the bacteria.

[0051] One end of the air inlet pipe 332 away from the pump body 331 is fixedly connected to a filter 335, and the filter 335 is used to filter impurities, microorganisms and other harmful substances in the air. The outer side of the filter 335 is fixedly connected to a support plate 336, and the side of the support plate 336 away from the filter 335 is fixedly connected to the top of the base plate 1. The filter 335 is provided to increase the purity of the air entering the shell 2 and prevent impurities, microorganisms and other harmful substances in the air from affecting the reproduction of bacteria.

[0052] A circular plate 337 is fixedly connected to the inner side surface of the cylinder 3310, and the circular plate 337 is arranged on the side of the oxygen tube 333 away from the bottom plate 1, and the end of the electric heating wire 334 away from the bottom plate 1 is fixedly connected to the side of the circular plate 337 close to the bottom plate 1, and a plurality of holes are evenly opened on the surface of the circular plate 337, and the hole wall of the circular plate 337 is fixedly connected to the gas injection pipe 338, and the end of the gas injection pipe 338 away from the circular plate 337 is bent toward the side close to the circular plate 337, and the gas injection pipe 338 is set in a U-shape, and an air stone 339 is arranged inside the vertical pipe of the gas injection pipe 338 away from the circular plate 337, and the outer side surface of the air stone 339 is fixedly connected to the inner side surface of the gas injection pipe 338, and the air inside the oxygen tube 333 is injected into the cylinder 3310, and then enters the gas injection pipe 338, and finally enters the interior of the shell 2 after passing through the air stone 339. The setting of the air stone 339 can block the air injection pipe 338 to prevent the culture solution from entering the air injection pipe 338, and the air injection pipe 338 is set to an inverted U-shape, with one end located inside the solution. Due to the pressure and the continuous introduction of gas at the other end, it is difficult for the culture solution to enter the air injection pipe 338. The loose porous structure of the air stone 339 can generate a large number of bubbles when the gas is introduced. The bubbles can drive the solution to roll, so that the oxygen and heat in the gas are fully mixed with the culture solution, and the tumbling solution, the nutrients therein and the bacteria can be fully mixed to achieve a similar stirring effect. While providing oxygen for the reproduction of the bacteria, the present invention does not need to set a stirring structure of the traditional culture equipment.

[0053] Example 2, please refer to Figure 1-Figure 8 The exhaust assembly 36 includes an exhaust pipe 361, which is arranged outside the shell 2. One end of the exhaust pipe 361 close to the shell 2 extends to the inside of the shell 2, and the outer side of the exhaust pipe 361 is fixedly connected to the inner side of the shell 2. The exhaust pipe 361 is controlled by a valve. Due to the injection of air, the pressure inside the shell 2 rises, and the air inside the shell 2 is finally pressed into the exhaust pipe 361. Since the exhaust pipe 361 is arranged at the lower part of the remaining inner cavity after the shell 2 is filled with grape juice, the carbon dioxide with a higher density will be preferentially discharged from the outside of the shell 2.

[0054] An exhaust pipe 362 is fixedly connected to the inner side of the outer shell 2. The exhaust pipe 362 is hollow and made of an elastic material. A tube body 363 is arranged inside the exhaust pipe 362. The end of the tube body 363 close to the center of the outer shell 2 passes through the exhaust pipe 362, and the outer side of the tube body 363 is fixedly connected to the inner side of the exhaust pipe 362. There are several tube bodies 363 evenly distributed along the circumference of the exhaust pipe 362. One end of the tube body 363 located outside the exhaust pipe 362 is fixedly connected to a film 364, and the film 364 is made of a hydrophobic material. A fixed sleeve 365 is arranged inside the exhaust pipe 362. The outer side of the fixed sleeve 365 is fixedly connected to the inner side of the exhaust pipe 362, and the inner side of the fixed sleeve 365 is fixedly connected to the outer side of the tube body 363, and the fixed sleeve 365 is made of an elastic material. Air passes through the film 364 and then enters The carbon dioxide enters the tube body 363, enters the inner cavity of the exhaust pipe 362, and finally enters the exhaust pipe 361, and finally discharges the outer shell 2 to balance the pressure and discharge excess carbon dioxide. The film 364 is made of hydrophobic material, which can prevent the solution from entering the tube body 363 and avoid waste when dumping and unloading. Since the exhaust pipe 362 is made of elastic material, when the outer shell 2 is placed upright, the exhaust pipe 362 is conical under the action of gravity, which can facilitate the loading of materials from the loading pipe 32. The raw materials enter the top of the circular plate 337. When unloading, the device is tipped and the device is turned upside down. Under the action of gravity, the exhaust pipe 362 is conical in the other direction, which is convenient for concentrated unloading. When unloading, it is not easy to contaminate the remaining inner wall of the outer shell 2, thereby reducing the loss of bacteria after breeding. Under the mass action of the tube body 363, etc., the exhaust pipe 362 is counterweighted to make the conical structure more obvious and increase the smoothness of loading and unloading.

[0055] When in use, grape juice and activated mother bacteria solution are added to the inside of the shell 2 from the two feeding pipes 32 respectively, the valve of the exhaust pipe 361 is opened, the pump body 331 and the electric heating wire 334 are started, and the pump body 331 drives the external oxygen to pass through the filter 335 to filter the impurities, microorganisms and other harmful substances in the air, and then the filtered air passes through the air intake pipe 332, the pump body 331, and the oxygen pipe 333 in turn. The electric heating wire 334 generates heat to heat the oxygen passing through the oxygen pipe 333 and heats it to a suitable temperature. The heated filtered air is injected into the cylinder 3310, and then enters the gas injection pipe 338, and finally enters the inside of the shell 2 after passing through the air stone 339. The loose porous structure of the air stone 339 generates a large number of bubbles when the gas is passed, and the bubbles drive the solution Tumbling allows the oxygen and heat in the gas to be fully mixed with the culture solution, and the nutrients in the solution are also fully mixed with the bacteria, achieving a similar stirring effect. Due to the injection of air, the pressure inside the shell 2 rises, and the air inside the shell 2 passes through the film 364, then enters the tube body 363, enters the inner cavity of the exhaust pipe 362, and finally enters the exhaust pipe 361, and finally exits the shell 2 to balance the pressure and discharge excess carbon dioxide. Before breeding, when the shell 2 is placed upright, the exhaust pipe 362 is conical under the action of gravity. When loading from the loading pipe 32, the raw materials follow the conical exhaust pipe 362 into the top of the circular plate 337. When unloading, the device is tilted and turned upside down. Under the action of gravity, the exhaust pipe 362 is deformed in the other direction to become conical, and the unloading is concentrated without contaminating the remaining inner wall of the shell 2.

[0056] Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field and related fields without creative work should fall within the scope of protection of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention are implemented according to the conventional means in the field unless otherwise specified and limited.

Claims

1. A bacterial strain culture device for wine brewing, characterized in that: include: A bottom plate (1), a shell (2) being arranged on the top of the bottom plate (1); A culture mechanism (3), wherein the culture mechanism (3) is fixedly connected to the top of the bottom plate (1), the top of the culture mechanism (3) is fixedly connected to the bottom of the outer shell (2), and a part of the culture mechanism (3) is fixed to the inner side of the outer shell (2); Wherein, the cultivation mechanism (3) comprises: A top cover (31), the top cover (31) is arranged on a side of the outer shell (2) away from the bottom plate (1), and the inner side surface of the top cover (31) is threadedly connected to the outer side surface of the outer shell (2), and two feeding pipes (32) are symmetrically arranged on the top of the top cover (31), and the ends of the two feeding pipes (32) close to the outer shell (2) both penetrate the top cover (31), and the inner side surface of the top cover (31) is fixedly connected to the outer surface of the feeding pipe (32), and the two feeding pipes (32) are both controlled by valves and are used for feeding nutrients and mother seeds respectively; An oxygen supply component (33) having a heating structure for providing a suitable temperature and oxygen for bacterial reproduction, wherein the oxygen supply component (33) is fixedly connected to the top of the bottom plate (1), and a side of the oxygen supply component (33) away from the bottom plate (1) is fixedly connected to a side of the housing (2) close to the bottom plate (1); A branch pipe (34), wherein the branch pipe (34) is arranged outside the outer shell (2), and one end of the branch pipe (34) close to the bottom plate (1) extends to the inside of the oxygen supply component (33), the outer side surface of the branch pipe (34) is fixedly connected to the inner side surface of the oxygen supply component (33), and the inner cavity of the branch pipe (34) is communicated with the oxygen supply pipeline of the oxygen supply component (33), and one end of the branch pipe (34) away from the bottom plate (1) extends to the inside of the outer shell (2), the outer side surface of the branch pipe (34) is fixedly connected to the inner side surface of the outer shell (2), and the inner cavity of the branch pipe (34) is communicated with the inner cavity of the outer shell (2); An exhaust component (36) has an opening and closing structure and is used to exhaust carbon dioxide generated by bacterial growth. The outer side surface of the exhaust component (36) is fixedly connected to the inner side surface of the outer shell (2).

2. The bacterial strain cultivation equipment for wine brewing according to claim 1, characterized in that: The end of the branch pipe (34) away from the bottom plate (1) penetrates one half of the outer shell (2) and is slightly above the upper position, and the exhaust assembly (36) is arranged on the outer shell (2) slightly below the position penetrated by the branch pipe (34).

3. The bacterial strain cultivation equipment for wine brewing according to claim 2, characterized in that: One end of the branch pipe (34) located inside the outer shell (2) is fixedly connected to a ring tube (35); the ring tube (35) is embedded inside the outer shell (2), and the outer side surface of the ring tube (35) is fixedly connected to the inner side surface of the outer shell (2); the ring tube (35) is hollow, and the inner cavity of the ring tube (35) is connected to the inner cavity of the outer shell (2) and the inner cavity of the branch pipe (34); the outer side surface of the ring tube (35) close to the center of the outer shell (2) is made of a hydrophobic material.

4. The bacterial strain cultivation equipment for wine brewing according to claim 3, characterized in that: The oxygen supply assembly (33) comprises a pump body (331), one end of the pump body (331) away from the center of the bottom plate (1) is fixedly connected to an air intake pipe (332), one end of the pump body (331) away from the air intake pipe (332) is fixedly connected to an oxygen pipe (333), and one end of the oxygen pipe (333) away from the pump body (331) is located directly below the outer shell (2).

5. The bacterial strain cultivation equipment for wine brewing according to claim 4, characterized in that: A cylinder (3310) is disposed outside the air inlet pipe (332), the cylinder (3310) is made of a heat-insulating material, and the cylinder (3310) is fixedly connected to the top of the bottom plate (1), a side of the cylinder (3310) away from the bottom plate (1) is fixedly connected to a side of the housing (2) close to the bottom plate (1), and an end of the pump body (331) close to the oxygen pipe (333) is fixedly connected to the outer side of the cylinder (3310), and One end of the oxygen pipe (333) close to the pump body (331) passes through the cylinder (3310), and the outer side surface of the oxygen pipe (333) is fixedly connected to the inner side surface of the cylinder (3310). The branch pipe (34) extends into the interior of the cylinder (3310), and one end of the branch pipe (34) close to the bottom plate (1) is fixedly connected to the outer side surface of the oxygen pipe (333), and the inner cavity of the branch pipe (34) is connected to the inner cavity of the oxygen pipe (333).

6. The bacterial strain cultivation equipment for wine brewing according to claim 5, characterized in that: The oxygen tube (333) is bent into a spiral shape inside the cylinder (3310), and a heating wire (334) is sheathed on the outside of the oxygen tube (333). The heating wire (334) is arranged in a spiral shape, and the end surface of the heating wire (334) is fixedly connected to the bottom of the inner side surface of the cylinder (3310).

7. The bacterial strain cultivation equipment for wine brewing according to claim 6, characterized in that: One end of the air inlet pipe (332) away from the pump body (331) is fixedly connected to a filter (335), and the filter (335) is used to filter impurities and microorganisms in the air. The outer side of the filter (335) is fixedly connected to a support plate (336), and the side of the support plate (336) away from the filter (335) is fixedly connected to the top of the bottom plate (1).

8. The bacterial strain cultivation equipment for wine brewing according to claim 7, characterized in that: The inner side surface of the cylinder (3310) is fixedly connected to a circular plate (337), and the circular plate (337) is arranged on a side of the oxygen tube (333) away from the bottom plate (1). The end of the electric heating wire (334) away from the bottom plate (1) is fixedly connected to the side of the circular plate (337) close to the bottom plate (1). The surface of the circular plate (337) is evenly provided with a plurality of holes. The hole walls of the circular plate (337) are fixedly connected to an air injection pipe (338), and the end of the air injection pipe (338) away from the circular plate (337) is bent toward the side close to the circular plate (337). The air injection pipe (338) is arranged in a U-shape. An air stone (339) is arranged inside the vertical pipe of the air injection pipe (338) away from the circular plate (337), and the outer side surface of the air stone (339) is fixedly connected to the inner side surface of the air injection pipe (338).

9. The bacterial strain cultivation equipment for wine brewing according to claim 8, characterized in that: The exhaust assembly (36) comprises an exhaust pipe (361), the exhaust pipe (361) being arranged outside the outer shell (2), one end of the exhaust pipe (361) close to the outer shell (2) extending into the interior of the outer shell (2), and the outer side surface of the exhaust pipe (361) being fixedly connected to the inner side surface of the outer shell (2), and the exhaust pipe (361) being controlled by a valve.

10. The bacterial strain cultivation equipment for wine brewing according to claim 9, characterized in that: The inner side surface of the outer shell (2) is fixedly connected with an exhaust pipe (362), the exhaust pipe (362) is hollow, and the exhaust pipe (362) is made of elastic material, a tube body (363) is arranged inside the exhaust pipe (362), one end of the tube body (363) close to the center of the outer shell (2) passes through the exhaust pipe (362), and the outer side surface of the tube body (363) is fixedly connected with the inner side surface of the exhaust pipe (362), and the tube body (363) is uniformly arranged along the circumference of the exhaust pipe (362). There are several of them distributed, one end of the tube body (363) located outside the exhaust pipe (362) is fixedly connected with a film (364), and the film (364) is made of a hydrophobic material, and a fixing sleeve (365) is arranged inside the exhaust pipe (362), the outer side surface of the fixing sleeve (365) is fixedly connected to the inner side surface of the exhaust pipe (362), and the inner side surface of the fixing sleeve (365) is fixedly connected to the outer side surface of the tube body (363), and the fixing sleeve (365) is made of an elastic material.

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