Nitrosomonas sp. with high ammonia oxidation activity and application thereof

Abstract

The application discloses a nitrosomonas sp. strain with high ammonia oxidation activity and application thereof, and relates to the technical field of microorganisms and environmental protection. The strain is preserved in the Guangdong Provincial Microbial Culture Collection Center on October 21, 2024, and is named as nitrosomonas sp. LY-1, with a preservation number of GDMCC No: 65307. The strain LY-1 is a new species, expands the species and diversity of the nitrosomonas sp. strain of ammonia-oxidizing bacteria, can perform biological denitrification under aerobic conditions, can quickly and efficiently convert ammonia nitrogen into nitrous nitrogen, and can be used for treating wastewater or soil containing high ammonia nitrogen, that is, the strain has the biological function of biological denitrification, and has a wide application prospect.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microorganism and environmental protection, and particularly relates to a nitrosomonas with high ammonia oxidation activity and application thereof. BACKGROUND

[0002] Nitrogen is essential for life and food production. Despite this, nitrogen losses of unused nitrogen in wastewater, air, fresh water, and oceans have caused nitrogen pollution, which can affect the environment and lead to eutrophication, climate change, loss of biodiversity, and ozone depletion, and it can also cause human cardiopulmonary problems. Human activities such as food processing, fertilizer production, mining, etc. affect the global biogeochemical nitrogen cycle. The most important advantage of biological denitrification technology is that the biological treatment process can be carried out at ambient temperature and atmospheric pressure, is simple to operate, has low capital and operating costs but high treatment efficiency, and is more ecological than physical and chemical methods, making up for the high cost and production of undesirable by-products of chemical and physical methods.

[0003] Ammonia oxidation reaction is the first step of nitrification and is also the rate-limiting step. Ammonia-oxidizing bacteria (AOB) are one of the important microorganisms involved in the process of ammonia oxidation reaction. Since the first isolation of AOB in 1890, a large number of studies have been conducted to determine its phylogenetic diversity. Through these efforts, 5 AOB genera have been identified and classified into the class of Proteobacteria. Four of them belong to the subclass of beta-Proteobacteria, including Nitrosomonas (including Nitrosomonas mobilis), Nitrosospira, Nitrosolobus, and Nitrosococcus, while one Nitrosomonas cluster belongs to the subclass of alpha-Proteobacteria. However, due to the complexity of the investigation of the count, diversity, and abundance of AOB in engineering systems or their natural environment, the use of traditional culture-dependent techniques is still a complex process.

[0004] In summary, due to the difficulty in isolating and purifying AOB strains, it is difficult to obtain AOB strains. The isolation and screening of ammonia-oxidizing bacteria are beneficial to the types and diversity of ammonia-oxidizing bacteria, increase the application potential of chemoautotrophic ammonia-oxidizing bacteria, and the research on such functional microorganisms will also provide microbial resource guarantee for ecological environment governance and resource geochemistry cycle. SUMMARY

[0005] In order to overcome the shortcomings and deficiencies of the prior art, the primary purpose of the present application is to provide a nitrosomonas with high ammonia oxidation activity, which solves the problems of few types and small amount of isolated ammonia-oxidizing bacteria.

[0006] Another purpose of the present application is to provide the application of the above-mentioned nitrosomonas.

[0007] The purpose of this invention is to obtain a Nitrosomonas sp. LY-1 strain by controlling the O2 concentration in sludge samples in liquid culture media and solid plates to promote faster and better growth of the AOB strain. Experiments have confirmed that this strain exhibits a strong and rapid ability to convert ammonia nitrogen to nitrite nitrogen, thus it can be used for ammonia nitrogen removal and has broad application prospects.

[0008] The objective of this invention is achieved through the following technical solution:

[0009] A strain of Nitrosomonas with high ammonia oxidation activity, named Nitrosomonas sp. LY-1, with accession number GDMCC No: 65307, was deposited on October 21, 2024, at the Guangdong Provincial Microbial Culture Collection Center of the Institute of Microbiology, Guangdong Academy of Sciences, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, Guangdong Province, China.

[0010] The application of the aforementioned Nitrosomonas bacteria with high ammonia oxidation activity in ammonia nitrogen removal, and further in the conversion of ammonia nitrogen to nitrite nitrogen; preferably includes the following steps: placing the aforementioned Nitrosomonas bacteria with high ammonia oxidation activity in a container containing ammonia nitrogen (NH4) + It grows in an environment with -N).

[0011] The growth is carried out at an oxygen concentration of 3-21%; more specifically, at an oxygen concentration of 5-21%.

[0012] The ammonia nitrogen mentioned includes at least one of ammonia nitrogen compounds such as ammonium chloride, ammonium sulfate, and urea.

[0013] The initial ammonia nitrogen concentration in the environment is 1–100 mM; preferably 1.5–100 mM; further, 1.5–50 mM; even further, 5–50 mM; and still further, 5–10 mM.

[0014] The salinity of the environment is 0–200 mM (excluding 200 mM); further, it is 0–100 mM.

[0015] The environment preferably includes at least one of water and soil.

[0016] The growth temperature is 18–37°C; more preferably 30–37°C.

[0017] The present invention has the following advantages and effects compared with the prior art:

[0018] The energy autotrophic Nitrosomonas LY-1 provided by the application is a new species, which expands the species and diversity of ammonia-oxidizing bacteria Nitrosomonas, and the strain LY-1 can carry out biological denitrification under aerobic conditions, and can quickly and efficiently convert ammonia nitrogen into nitrous nitrogen, and can be used for treating wastewater or soil containing high ammonia nitrogen, i.e., the strain has the biological function of biological denitrification. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 The colonies and transparent circles formed by the ammonia-oxidizing bacteria enrichment liquid of the sludge sample of Longtan Park of Liuzhou City in a double-layer serum bottle sealed solid plate containing CaCO3 and 5% O2 headspace; wherein, left: cultured for 10d; right: cultured for 30d.

[0020] Figure 2 It is a phylogenetic tree diagram based on 16S rDNA of Nitrosomonas LY-1.

[0021] Figure 3 It is a cell morphology and colony morphology diagram of Nitrosomonas LY-1; wherein, A is a gram staining photo (100x), scale: 200pixel; B is a colony morphology photo.

[0022] Figure 4 It is an ammonia oxidation metabolism and growth curve result diagram of Nitrosomonas LY-1 under the growth conditions of normal oxygen, 1.5mM NH4Cl and 2.5mM NaHCO3.

[0023] Figure 5 It is nitrous nitrogen generation of Nitrosomonas LY-1 under the growth conditions of normal oxygen, 1.5mM NH4Cl, 2.5mM NaHCO3 and 7 different NaCl concentrations (10, 50, 100, 200, 300, 400, 500mM).

[0024] Figure 6 It is nitrous nitrogen generation of Nitrosomonas LY-1 under the growth conditions of normal oxygen, 2.5mM NaHCO3 and 6 different NH4Cl concentrations (1, 5, 10, 20, 50, 100mM).

[0025] Figure 7 It is nitrous nitrogen generation of Nitrosomonas LY-1 under the growth conditions of normal oxygen, 1.5mM NH4Cl, 2.5mM NaHCO3 and 4 different culture temperatures (18, 20, 30, 37℃). DETAILED DESCRIPTION

[0026] The application will be further described in conjunction with the following examples and drawings, but the embodiments of the application are not limited thereto. The test methods in the following examples, unless otherwise specified, are generally in accordance with conventional test methods or as suggested by the manufacturer. The materials, reagents and the like used are commercially available unless otherwise specified.

[0027] Example 1

[0028] Enrichment, screening, isolation and identification of autotrophic nitrifying bacteria

[0029] (1) Enrichment culture of ammonia-oxidizing bacteria: A sludge sample was collected from a site (E110°01', N23°29') in Longtan Park, Liuzhou City, using a sampler, and was placed in a 50 mL centrifuge tube for standby. 5 mL of the sludge sample was inoculated into 40 mL of inorganic medium containing CaCO3 for enrichment culture, and was cultured at 30°C in a 150 rpm shaker in the dark. The ammonia nitrogen consumption and nitrite nitrogen production were determined every 2 days, and the culture was repeatedly transferred for 3-6 generations under the same conditions to obtain a stable ammonia-oxidizing bacteria (AOB) population. The composition of the inorganic medium containing CaCO3 was as follows: MgSO4·7H2O 0.05 g / L, KH2PO4 0.1 g / L, NaCl 0.5 g / L (8.5 mM), CaCO3 0.5 g / L, NaHCO3 0.21 g / L (2.5 mM), NH4Cl 5-10 mM, trace element solution 1 mL / L. The composition of the trace element solution was as follows: EDTA-Na2 5.2 g / L, FeSO4·7H2O 2.1 g / L, H3BO3 0.03 g / L, CuCl2·2H2O 0.002 g / L, MnCl2·4H2O 0.1 g / L, Na2MoO4·2H2O 0.036 g / L, ZnSO4 0.144 g / L, CoCl2·6H2O 0.19 g / L, NiCl2·6H2O 0.024 g / L, and the pH was adjusted to 6.0 with NaOH. The NaHCO3, NH4Cl stock solution, and trace element solution were sterilized by filtration through a 0.22 μm filter membrane and added to the sterilized liquid medium.

[0030] (2) Isolation and purification of ammonia-oxidizing bacteria: After obtaining the stable ammonia-oxidizing bacteria colony in step (1) above, 100 μL of the enrichment liquid is taken, 10-fold gradient diluted, and then plated (the upper layer is a solid inorganic medium containing CaCO3), sealed with butyl silica gel plug and aluminum cap, and then the headspace air is pumped out with a vacuum pump and replaced with nitrogen to make the oxygen concentration in the headspace of the sealed serum bottle solid plate 5%. Then the serum bottle is placed upright in a 30°C constant temperature incubator in the dark. After 10 days and 30 days of culture, AOB colonies grow on the culture medium and form transparent circles around the colonies Figure 1 . The above transparent circles are picked and streaked on the sealed solid plate of the serum bottle, and the oxygen concentration in the headspace is also controlled at 5%. After 10 days of culture, single colonies grow on the plate, which can be picked.

[0031] The above colonies are picked and cultured in the inorganic medium containing CaCO3 in the dark at 30°C and 150 rpm on a shaker, and the ammonia nitrogen consumption and nitrite nitrogen generation are detected every day. If nitrite nitrogen is steadily generated, it can be preliminarily determined that it is the target ammonia-oxidizing bacteria.

[0032] The solid inorganic medium containing CaCO3: 15-20 g / L agar is added to the inorganic medium containing CaCO3. After high-temperature sterilization, NaHCO3, NH4Cl mother liquor and trace element solution filtered with a 0.22 μm filter to remove bacteria are added. 30-40 mL of the solid inorganic medium containing CaCO3 is poured into a serum bottle, sealed with a sealing film, and blue glass sand (25 g / bottle) is shaken evenly to make it horizontally flat on the side of the bottle. The bottle is placed and waits for the agar to solidify.

[0033] NH4 + detection:

[0034] 1. Staining solution A: 50 mL water + 8.5 g sodium salicylate + 0.6 g NaOH + 63.9 mg nitrosyl ferricyanide sodium dihydrate;

[0035] 2. Staining solution B: 0.2 g dichloroisocyanuric acid sodium + 50 mL water;

[0036] 3. 100 μL sample + 100 μL staining solution A + 100 μL staining solution B + 1700 μL water;

[0037] 4. The absorbance is measured at a wavelength of 660 nm after color development for 30 min.

[0038] NO2 - detection:

[0039] 1. Dye solution: 600 mL water, 200 mL concentrated phosphoric acid, 80 g sulfanilamide, 1000 mL volumetric flask, 4°C storage for 1 year; N-(1-naphthyl)-ethylenediamine hydrochloride solution: 0.4 g N-(1-naphthyl)-ethylenediamine hydrochloride is dissolved in 100 mL water, 4°C storage, and the solution is re-prepared when it becomes concentrated; color developing agent: 20 mL sulfanilamide solution + 20 mL N-(1-naphthyl)-ethylenediamine hydrochloride solution + 20 mL concentrated phosphoric acid in 100 mL brown bottle, 4°C storage, and the solution is re-prepared when it becomes dark;

[0040] 2. 100 μL bacterial supernatant, 1850 μL water, 50 μL color developing agent;

[0041] 3. After 10 min, the absorbance is measured at a wavelength of 543 nm.

[0042] (3) 16S rDNA identification of strain LY-1: the single colony obtained above which can perform normal ammonia oxidation reaction in the inorganic culture medium containing CaCO3 is picked to perform PCR amplification using 16S rDNA universal primers 27F / 1492R. The PCR reaction system is: single colony, 0.5 μL of upstream primer (10 μmol / L), 0.5 μL of downstream primer (10 μmol / L), 12.5 μL of DreamTaq Green PCR Master Mix (2x), and ddH2O to 25 μL. The PCR reaction conditions are: 94°C pre-denaturation for 5 min; 94°C denaturation for 30 s, 54°C annealing for 30 s, 72°C extension for 90 s, 35 cycles; 72°C extension for 10 min. The PCR product obtained is stored in a 4°C refrigerator. The PCR product is sent to Guangzhou Tianyi Huirong Gene Technology Co., Ltd. for 16S rDNA sequencing, and the results are as follows:

[0043] 27F: 5'-AGAGTTTGATCCTGGCTCAG-3';

[0044] 1492R: 5'-GGTTACCTTGTTACGACTT-3'.

[0045] Sequencing results:

[0046]

[0047] The strain is AOB by 16S rDNA Blast analysis, and phylogenetic tree analysis shows that AOB strain LY-1 is most similar to Nitrosomonas nitrosa, and the similarity of 16S rDNA of strain LY-1 with N. nitrosa, N. communis, N. aestuarii, N. halophila, N. marina and Nitrosopira sp. is 97.91%, 97.14%, 93.75%, 93.67%, 93.68% and 93.43% respectively, according to the reference (Kook JK, et al. Genome-Based Reclassification of Fusobacterium nucleatum Subspecies at the Species Level. Curr Microbiol (2017) 74: 1137-1147.), the 16S rDNA sequence similarity of 98.65% can be used as the threshold for two kinds of distinction, therefore, the strain LY-1 is a new species of Nitrosomonas genus ( Figure 2 ). The strain is named Nitrosomonas sp. LY-1 and preserved in Guangdong Microbial Culture Collection Center (GDMCC), with the preservation number of GDMCC No: 65307, the preservation time of October 21, 2024, and the preservation address of 5th floor, No. 59 Building, Institute of Microbiology, Guangdong Academy of Sciences, 100 Middle Martyrs Road, Guangzhou, Guangdong, China.

[0048] In summary, a strain LY-1 is obtained, the colony morphology and gram staining photo under 100x oil lens of the strain are as shown in Figure 3 , the bacterial body is short rod-shaped, the colony is colorless and transparent (cultured for 10d), the edge is complete, the surface is glossy, it does not produce spores, it is gram staining negative bacteria, and it is a strict chemolithoautotroph, the optimum growth temperature is 30-37℃, and the optimum growth pH is 7.0-7.5.

[0049] The carbon source and nitrogen source utilization of the strain LY-1 is shown in Table 1. The data in Table 1 shows that the strain LY-1 can grow and metabolize with ammonium chloride, urea, ammonium sulfate, etc. as substrate; it can grow and metabolize with lactic acid, glycerol, sodium pyruvate, sodium bicarbonate, etc. as carbon source, among which sodium bicarbonate is the best; the strain LY-1 cannot grow and metabolize with organic carbon sources such as sodium formate, sodium acetate, methanol and ethanol.

[0050] Table 1 Carbon source and nitrogen source utilization of LY-1

[0051]

[0052] Note: "+" is positive, "-" is negative; under normal oxygen conditions.

[0053] Example 2

[0054] The results of the detection of the nitrogen metabolism characteristics and growth characteristics of Nitrosomonas sp. LY-1 in the CaCO3-containing inorganic medium containing 1.5 mM NH4Cl and 2.5 mM NaHCO3 under normal oxygen conditions are as follows:

[0055] The Nitrosomonas sp. LY-1 single colony obtained by the above separation, purification and identification was picked and inoculated in the CaCO3-containing inorganic medium (see Example 1 for components) containing 1.5 mM NH4Cl and 2.5 mM NaHCO3 for activation under normal oxygen conditions. When the concentration of nitrite nitrogen generated was 0.6 mM, it was transferred into fresh liquid medium containing 1.5 mM NH4Cl and 2.5 mM NaHCO3 at a 5% inoculation amount under normal oxygen conditions. The experimental group was placed in a 30°C, 150 rpm shaking incubator for dark shaking culture. Samples were taken every 12 hours to measure ammonia nitrogen consumption, nitrite nitrogen generation, and cell count by plating. Each parallel group had three sets, and the total reaction time was 108 h.

[0056] The results of the detection of the nitrogen metabolism characteristics and growth characteristics of Nitrosomonas sp. LY-1 in the CaCO3-containing inorganic medium containing 1.5 mM NH4Cl and 2.5 mM NaHCO3 under normal oxygen conditions are as follows: Figure 4 As shown in the results, with the increase of culture time, the concentration of ammonia nitrogen continuously decreased, the concentration of nitrite nitrogen continuously increased, and the amount of cell growth also continuously increased, reaching 2.1 x 10 6 CFU / mL at 108 h.

[0057] Example 3

[0058] The results of the detection of the ammonia oxidation metabolism characteristics of Nitrosomonas sp. LY-1 in the CaCO3-containing inorganic medium containing 1.5 mM NH4Cl, 2.5 mM NaHCO3, and different concentrations of NaCl (10, 50, 100, 200, 300, 400, and 500 mM) under normal oxygen conditions are as follows:

[0059] The single colony of Nitrosomonas sp. LY-1 obtained by the above separation, purification and identification was inoculated into the CaCO3-containing inorganic medium containing 1.5 mM NH4Cl and 2.5 mM NaHCO3 for activation under normal oxygen condition. When the concentration of nitrous nitrogen generated by the bacteria was 0.6 mM, the bacteria were inoculated into fresh liquid medium containing 1.5 mM NH4Cl and 2.5 mM NaHCO3 with different concentrations of NaCl (10, 50, 100, 200, 300, 400 and 500 mM) at a 5% inoculation amount under normal oxygen condition. The experimental groups were placed in a shaker at 30°C and 150 rpm for culture under light-avoiding and shaking. Samples were taken every 24 hours for determination of ammonia nitrogen consumption and nitrous nitrogen generation. Each parallel group was in triplicate, and the total reaction time was 6 days.

[0060] The ammonia oxidation metabolic characteristics of Nitrosomonas sp. LY-1 in the CaCO3-containing inorganic medium containing 1.5 mM NH4Cl, 2.5 mM NaHCO3 and different concentrations of NaCl (10, 50, 100, 200, 300, 400 and 500 mM) under normal oxygen condition are shown in Table 1. Figure 5 The results show that when the concentration of NaCl is ≥200 mM, LY-1 stops growing and metabolizing; when the concentration of NaCl is <200 mM, LY-1 grows and metabolizes normally, and when the concentration of NaCl is 50 mM, the ammonia oxidation rate of LY-1 is optimal.

[0061] Example 4

[0062] The ammonia oxidation characteristics of Nitrosomonas sp. LY-1 in the CaCO3-containing inorganic medium containing 2.5 mM NaHCO3 and different concentrations of NH4Cl (1, 5, 10, 20, 50 and 100 mM) under normal oxygen condition are shown in Table 2.

[0063] The single colony of Nitrosomonas sp. LY-1 obtained by the above separation, purification and identification was inoculated into the CaCO3-containing inorganic medium containing 1.5 mM NH4Cl and 2.5 mM NaHCO3 for activation under normal oxygen condition. When the concentration of nitrous nitrogen generated by the bacteria was 0.6 mM, the bacteria were inoculated into fresh liquid medium containing different concentrations of NH4Cl (1, 5, 10, 20, 50 and 100 mM) and 2.5 mM NaHCO3 at a 5% inoculation amount under normal oxygen condition. The experimental groups were placed in a shaker at 30°C and 150 rpm for culture under light-avoiding and shaking. Samples were taken every 24 hours for determination of ammonia nitrogen consumption and nitrous nitrogen generation. Each parallel group was in triplicate, and the total reaction time was 8 days.

[0064] The ammonia oxidation metabolic characteristics of *Nitrosomonas sp.* LY-1 under normal oxygen conditions, in inorganic media containing 2.5 mM NaHCO3 and different concentrations of NH4Cl (1, 5, 10, 20, 50, 100 mM) in CaCO3-containing medium are as follows: Figure 6 As shown in the figure. The results indicate that when the NH4Cl concentration is >100 mM, the ammonia oxidation rate of LY-1 is inhibited. When the NH4Cl concentration is ≤100 mM, the ammonia oxidation rate of LY-1 increases with increasing substrate concentration, but the final product (nitrite concentration) remains at 5 mM. It is speculated that 2.5 mM NaHCO3 may only provide LY-1 with 5 mM NH4Cl for consumption.

[0065] Example 5

[0066] The ammonia oxidation characteristics of Nitrosomonas sp. LY-1 under normal oxygen conditions, in inorganic media containing 1.5 mM NH4Cl, 2.5 mM NaHCO3, and at different temperatures (18, 20, 30, 37 °C) and CaCO3 were determined as follows:

[0067] Single colonies of Nitrosomonas sp. LY-1, obtained through isolation, purification, and identification, were picked and inoculated into an inorganic medium containing CaCO3 with 1.5 mM NH4Cl and 2.5 mM NaHCO3 for activation. Under normal oxygen conditions, when the nitrite nitrogen concentration reached 0.6 mM, 5% of the inoculum was transferred to fresh liquid medium containing 1.5 mM NH4Cl and 2.5 mM NaHCO3 at different temperatures (18, 20, 30, and 37 °C). Under normal oxygen conditions, the experimental groups were placed in a shaker at 30 °C and 150 rpm in the dark for shaking culture. Samples were taken every 24 hours to measure ammonia nitrogen consumption and nitrite nitrogen production. Each group was divided into three parallel groups, and the total reaction time was 6 days.

[0068] The ammonia oxidation metabolic characteristics of *Nitrosomonas* sp. LY-1 under normal oxygen conditions, in inorganic media containing 1.5 mM NH4Cl, 2.5 mM NaHCO3, and at different temperatures (18, 20, 30, 37 °C) and CaCO3 are shown in the following results. Figure 7 As shown in the figure. The results indicate that with the increase of culture temperature, the ammonia nitrogen concentration continuously decreases while the nitrite nitrogen concentration continuously increases, and the ammonia oxidation rate is: 37℃ > 30℃ > 18℃ > 20℃.

[0069] The above embodiments are the preferred embodiments of the present application, but the embodiments of the present application are not limited to the above embodiments, and any changes, modifications, substitutions, combinations, simplifications, etc. made without departing from the spirit and principles of the present application should be equivalent replacement manners and should be included in the protection scope of the present application.

Claims

1. A strain of Nitrosomonas having high ammonia oxidation activity, characterized in that: The strain is named Nitrosomonas ( Nitrosomonas sp.)LY-1, with accession number GDMCC No:65307, was deposited on October 21, 2024 at the Guangdong Provincial Microbial Culture Collection Center, Institute of Microbiology, Guangdong Academy of Sciences, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, Guangdong Province, China.

2. The use of the Nitrosomonas having high ammonia oxidation activity according to claim 1 in the removal of ammonia nitrogen.

3. Use according to claim 2, characterized in that: In the use in the conversion of ammonia nitrogen to nitrite nitrogen.

4. Use according to claim 2 or 3, characterized in that: It comprises the step of growing the Nitrosomonas having high ammonia oxidation activity according to claim 1 in an environment containing ammonia nitrogen.

5. The use according to claim 4, wherein: The ammonia nitrogen comprises at least one of ammonium chloride, ammonium sulfate and urea.

6. The use according to claim 4, wherein: The growth is at an oxygen concentration of 3-21%.

7. The use according to claim 6, wherein: The growth is at an oxygen concentration of 5-21%.

8. Use according to claim 4, characterized in that: The initial ammonia nitrogen concentration in the environment is 1-100 mM.

9. Use according to claim 8, characterized in that: The initial ammonia nitrogen concentration in the environment is 1.5-100 mM.

10. Use according to claim 9, characterized in that: The initial ammonia nitrogen concentration in the environment is 1.5-50 mM.

11. Use according to claim 10, characterized in that: The initial ammonia nitrogen concentration in the environment is 5-50 mM.

12. Use according to claim 11, characterized in that: The initial ammonia nitrogen concentration in the environment is 5-10 mM.

13. The use according to claim 4, wherein: The salinity of the environment is 0-200 mM, not containing 200 mM.

14. The use according to claim 13, characterized in that: The salinity of the environment is 0-100 mM.

15. The use according to claim 4, wherein: The environment comprises at least one of water body and soil.

16. The use according to claim 4, characterized in that: The temperature of the growth is 18-37 ℃.

17. Use according to claim 16, characterized in that: The temperature of the growth is 30-37 ℃.

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