Rapid propagation method for cunninghamia lanceolata by taking stem segment as explant

By using stem segments as explants and induction, proliferation and regenerating root in culture media with different formulas, the problem of single asexual reproduction mode of Luotian weeping clover is solved, and rapid reproduction and maintaining the excellent variety is achieved.

CN119678846APending Publication Date: 2025-03-25YANGTZE UNIVERSITY
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Patent Information

Application Number
CN202411771166.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-04
Publication Date
2025-03-25

AI Technical Summary

Technical Problem

The asexual reproduction method of Luotian weeping cypress is single, and the rapid reproduction system based on plant tissue culture technology is lacking, resulting in low reproduction efficiency and difficult to maintain the excellent variety.

Method used

The stem segment was used as an explant, and the culture medium of different formulas (MS+ agar+sucrose+6-BA+IBA, MS+ agar+sucrose+6-BA+IBA, 1/2MS+ agar+sucrose+SNP+NAA) was successfully established. The sterile seedling micro-propagation and rapid breeding system of Luotian Cypress Cyrus was successfully established.

Benefits of technology

It realizes the rapid reproduction of Luotian weeping cypress and the excellent maintenance of variety, has a high transplant survival rate, and provides a reliable asexual reproduction method.

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Abstract

The invention provides a cunninghamia lanceolata rapid propagation method using stem segments as explants, and belongs to the technical field of plant tissue culture, the method comprises the following steps: S1, using the stem segments of cunninghamia lanceolata as the explants, and inoculating the stem segments on a first culture medium for induction culture; s2, inoculating the adventitious buds obtained in the step S1 to a second culture medium for multiplication culture; s3, inoculating the adventitious buds obtained in the step S2 into a third culture medium for rooting induction culture; s4, transplanting the aseptic seedlings obtained in the step S3; wherein the first culture medium and the second culture medium contain 6-BA and IBA, and the third culture medium contains SNP and NAA. According to the method, the tender stem of the cunninghamia lanceolata is taken as the explant, a cunninghamia lanceolata aseptic seedling micropropagation seedling rapid propagation system is successfully established for the first time, the transplanting survival rate is extremely high, and a reliable way is provided for keeping the excellent characters of the cunninghamia lanceolata and rapidly propagating the cunninghamia lanceolata.
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