Atractylodes lancea tissue culture bottle and use method thereof

Abstract

The invention discloses a rhizoma atractylodis tissue culture bottle and a use method thereof, and belongs to the field of rhizoma atractylodis culture. A rhizoma atractylodis tissue culture bottle comprises a bottle body and further comprises a culture layer arranged in the bottle body. The threaded cover is in threaded connection with the bottle body; the mounting plate is fixedly arranged on one side of the bottle body; the controller is fixedly arranged on the mounting plate; the air pump is fixedly arranged on the mounting plate; the first exhaust pipe is fixedly arranged at the exhaust end of the air pump, and the first exhaust pipe is communicated with the bottle body; the adjusting box is fixedly arranged on the mounting plate, and the adjusting box is used for adjusting the temperature and humidity of gas flowing through the adjusting box; gas in the bottle body can flow conveniently, the temperature and humidity in the bottle body can be effectively adjusted, and the situation that the temperature and humidity of the gas in the bottle body are not uniform, and the culture effect on atractylodes lancea tissue is affected is prevented.

Description

Technical Field

[0001] The invention relates to the technical field of atractylodes lancea culture, and in particular to an atractylodes lancea tissue culture bottle and a use method thereof. Background Art

[0002] Atractylodes is a perennial herb of the genus Atractylodes in the Asteraceae family. Its dried rhizomes are used as medicine, which has the effects of drying dampness and strengthening the spleen, dispelling wind and cold, and improving eyesight. It can be used to treat dampness blocking the middle burner, rheumatism, blurred vision, etc. It is one of the commonly used medicinal materials in Chinese medicine. Wild Atractylodes resources are becoming increasingly depleted. In order to achieve large-scale and rapid reproduction of Atractylodes, tissue culture can provide high-quality Atractylodes seedlings. When cultivating them, they need to be cultivated in culture bottles.

[0003] The current culture bottles use bottled containers to culture Atractylodes tissues, which are then placed in a constant temperature and humidity environment. Since nutrient solution needs to be sprayed on the Atractylodes tissues, the humidity around the Atractylodes may easily become high after spraying the nutrient solution, which may affect the culture effect of the Atractylodes. Summary of the invention

[0004] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide an Atractylodes lancea tissue culture bottle and a method of using the same which can overcome the above problems or at least partially solve the above problems.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A kind of atractylodes tissue culture bottle, comprising: a bottle body, and also comprising: a culture layer, which is arranged in the bottle body; a threaded cover, which is threadedly connected to the bottle body; a mounting plate, which is fixedly arranged on one side of the bottle body; a controller, which is fixedly arranged on the mounting plate; an air pump, which is fixedly arranged on the mounting plate; a first air extraction pipe, which is fixedly arranged at the air extraction end of the air pump, and the first air extraction pipe is connected to the bottle body; a regulating box, which is fixedly arranged on the mounting plate, and the regulating box is used to regulate the temperature and humidity of the gas flowing through; a first exhaust pipe, which is fixedly arranged between the input end of the regulating box and the output end of the air pump; a detection box, which is arranged on the upper side of the regulating box, and a first connecting pipe is arranged between the regulating box and the detection box; a second temperature and humidity sensor, which is arranged in the detection box; a second connecting pipe, which is arranged between the detection box and the bottle body, and the second connecting pipe is connected to the bottle body.

[0007] Preferably, a first temperature and humidity sensor is provided on the culture layer, a pump is provided on one side of the bottle body, a water spray pipe is fixedly connected to the output end of the pump, a nozzle is fixedly connected to the water spray pipe, and the nozzle is provided in the bottle body.

[0008] Furthermore, the inner wall of the bottle body is provided with a plurality of first annular tubes and second annular tubes, the first annular tube is provided with an exhaust port, the second annular tube is provided with an exhaust port, the first annular tube is connected to the second connecting tube, and the second annular tube is connected to the first exhaust pipe.

[0009] In order to prevent excessive airflow, preferably, the exhaust ports are equidistantly arranged on the first annular tube, and the suction ports are equidistantly arranged on the second annular tube.

[0010] Preferably, the first annular tube is arranged on the upper side of the nozzle, and the second annular tube is arranged on the lower side of the nozzle.

[0011] Furthermore, a limiting ring is fixedly connected in the bottle body, the limiting ring matches the culture layer, a first electromagnet is fixedly connected to the lower side of the culture layer, and a second electromagnet corresponding to the first electromagnet is arranged in the bottle body.

[0012] In order to facilitate the collection of excess culture fluid, preferably, a collecting plate is fixedly connected to the lower side of the bottle body, a storage cavity is provided between the lower side of the collecting plate and the bottle body, a fixed tube is provided in the middle of the collecting plate, and a one-way valve is provided in the fixed tube.

[0013] In order to prevent water mist from the culture solution from entering the air pump, further, an impeller box is fixedly connected to the lower side of the mounting plate, two cavities are arranged in the impeller box, a partition is fixedly connected between the two cavities, a mesh plate is fixedly connected in one of the cavities, a water-absorbing sponge is fixedly connected on the mesh plate, a rotating shaft is rotatably arranged in the other cavity, and the rotating shaft passes through the partition and the mesh plate, an impeller group is arranged at the end of the rotating shaft away from the mesh plate, the cavity with the impeller group is connected to the first exhaust pipe, a third connecting pipe is fixedly connected between the two cavities, a pressing plate for squeezing the water-absorbing sponge is fixedly connected at the end of the rotating shaft close to the mesh plate, and a second exhaust pipe is fixedly connected between the cavity close to the pressing plate and the second annular tube.

[0014] In order to facilitate the reflux of the culture solution into the storage cavity, preferably, a reflux pipe is provided between the cavity close to the mesh plate and the storage cavity.

[0015] A method for using an atractylodes tissue culture bottle, mainly comprising the following steps:

[0016] Step 1: Place the Atractylodes tissue on the culture layer and spray an appropriate amount of culture solution;

[0017] Step 2: Cover with a screw cap for cultivation;

[0018] Step 3: Extract the gas in the bottle through the air pump, and then transport it into the bottle to make the gas inside flow;

[0019] Step 4: Detect the temperature and humidity of the gas in the detection box through the second temperature and humidity sensor, and then control the adjustment box to start through the controller to adjust the temperature and humidity of the gas.

[0020] Compared with the prior art, the present invention provides a tissue culture bottle of Atractylodes lancea, which has the following

[0021] Beneficial effects:

[0022] 1. The atractylodes lancea tissue culture bottle extracts gas from the bottle body through an air pump and then transports the gas into the bottle body, wherein the gas passes through a first exhaust pipe, a regulating box, a first connecting pipe, a detection box and a second connecting pipe in sequence, and the temperature and humidity of the gas are detected by a second temperature and humidity sensor in the detection box. When the temperature is lower than a set value, the temperature control mechanism in the regulating box is used to heat the gas. When the culture solution evaporates and causes the humidity to be higher than the set value, the drying mechanism in the regulating box is used to dry the gas to reach the set value, thereby facilitating the flow of gas in the bottle body, effectively regulating the temperature and humidity in the bottle body, and preventing the uneven temperature and humidity of the gas in the bottle body from affecting the culture effect of the atractylodes lancea tissue.

[0023] 2. When the gas in the Atractylodes lancea tissue culture bottle is extracted, the gas in the bottle evenly enters the second annular tube from multiple extraction ports, and then enters the air pump, thereby effectively preventing the excessive air flow velocity in the bottle from affecting the culture of the Atractylodes lancea tissue.

[0024] 3. For the Atractylodes lancea tissue culture bottle, when the air pump extracts the gas in the bottle body, the gas enters a cavity from the second air extraction pipe, then enters another cavity after being filtered through the water-absorbing sponge, and finally enters the air pump through the first air extraction pipe. The gas passing through drives the rotating shaft to rotate through the impeller group, and the rotating shaft drives the pressure plate to rotate, squeezing out part of the water in the water-absorbing sponge, and then the culture fluid enters the storage cavity along the reflux pipe, effectively preventing the water mist sprayed from the nozzle from being extracted by the air pump and then circulating, thereby effectively ensuring the temperature and humidity in the bottle body, and then effectively improving the culture effect of the Atractylodes lancea tissue.

[0025] The parts not involved in the device are the same as the existing technology or can be implemented by using the existing technology. The present invention facilitates the flow of gas in the bottle, effectively adjusts the temperature and humidity in the bottle, and prevents the uneven temperature and humidity of the gas in the bottle from affecting the culture effect of the Atractylodes tissue. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 A schematic diagram of the structure of an Atractylodes lancea tissue culture bottle proposed by the present invention;

[0027] Figure 2 A schematic cross-sectional view of an Atractylodes lancea tissue culture bottle provided by the present invention;

[0028] Figure 3 This is a schematic diagram of the unfolded structure of a collection plate and a culture layer in an Atractylodes lancea tissue culture bottle proposed by the present invention;

[0029] Figure 4 A kind of Atractylodes tissue culture bottle proposed by the present invention Figure 2 The enlarged schematic diagram of point A in the middle;

[0030] Figure 5 A kind of Atractylodes tissue culture bottle proposed by the present invention Figure 2 The enlarged schematic diagram of point B in the middle;

[0031] Figure 6 A kind of Atractylodes tissue culture bottle proposed by the present invention Figure 2 Enlarged schematic diagram of point C in the middle.

[0032] In the figure: 1, bottle body; 101, culture layer; 102, first temperature and humidity sensor; 103, limit ring; 104, first electromagnet; 105, second electromagnet; 106, collection plate; 107, fixed tube; 108, threaded cover; 109, storage chamber; 2, first annular tube; 201, exhaust port; 202, second annular tube; 203, air extraction port; 204, mounting plate; 205, controller; 206, air pump; 207, first air extraction pipe; 208 , first exhaust pipe; 209, adjustment box; 210, first connecting pipe; 211, second connecting pipe; 3, detection box; 301, second temperature and humidity sensor; 4, impeller box; 401, cavity; 402, third connecting pipe; 403, second exhaust pipe; 404, return pipe; 405, rotating shaft; 406, impeller assembly; 407, partition; 408, mesh plate; 409, water-absorbing sponge; 410, pressure plate; 5, nozzle; 501, pump; 502, water spray pipe. DETAILED DESCRIPTION

[0033] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0034] Example 1: Reference Figure 1-Figure 6A kind of atractylodes tissue culture bottle, comprising: a bottle body 1, and also comprising: a culture layer 101, which is arranged in the bottle body 1; a threaded cover 108, which is threadedly connected to the bottle body 1; a mounting plate 204, which is fixedly arranged on one side of the bottle body 1; a controller 205, which is fixedly arranged on the mounting plate 204; an air pump 206, which is fixedly arranged on the mounting plate 204; a first air extraction pipe 207, which is fixedly arranged at the air extraction end of the air pump 206, and the first air extraction pipe 207 is connected to the bottle body 1; an adjustment box 209, which is fixedly arranged on the mounting plate 204, and the regulating box 209 is used to regulate the temperature and humidity of the gas flowing through; the first exhaust pipe 208 is fixedly arranged between the input end of the regulating box 209 and the output end of the air pump 206; the detection box 3 is arranged on the upper side of the regulating box 209, and a first connecting pipe 210 is arranged between the regulating box 209 and the detection box 3; the second temperature and humidity sensor 301 is arranged in the detection box 3; the second connecting pipe 211 is arranged between the detection box 3 and the bottle body 1, and the second connecting pipe 211 is connected to the bottle body 1.

[0035] A first temperature and humidity sensor 102 is provided on the culture layer 101 , a pump 501 is provided on one side of the bottle body 1 , an output end of the pump 501 is fixedly connected to a water spray pipe 502 , a nozzle 5 is fixedly connected to the water spray pipe 502 , and the nozzle 5 is provided in the bottle body 1 .

[0036] A limit ring 103 is fixedly connected inside the bottle body 1 , and the limit ring 103 matches the culture layer 101 . A first electromagnet 104 is fixedly connected to the lower side of the culture layer 101 . A second electromagnet 105 corresponding to the first electromagnet 104 is arranged inside the bottle body 1 .

[0037] The regulating box 209 is provided with a drying mechanism and a temperature control mechanism.

[0038] When culturing the Atractylodes lancea tissue, the Atractylodes lancea tissue is placed on the culture layer 101, and a proper amount of culture solution is sprayed on the Atractylodes lancea tissue, and then the threaded cap 108 is covered for culturing.

[0039] During the culture process, the gas in the bottle body 1 is extracted by the air pump 206, and then the gas is transported to the bottle body 1, wherein the gas passes through the first exhaust pipe 208, the regulating box 209, the first connecting pipe 210, the detection box 3 and the second connecting pipe 211 in sequence, and the temperature and humidity of the gas are detected by the second temperature and humidity sensor 301 in the detection box 3. When the temperature is lower than the set value, the temperature control mechanism in the regulating box 209 is used to heat the gas. When the culture solution evaporates and causes the humidity to be higher than the set value, the drying mechanism in the regulating box 209 is used to dry the gas to reach the set value, so as to facilitate the flow of gas in the bottle body 1, effectively adjust the temperature and humidity in the bottle body 1, and prevent the uneven temperature and humidity of the gas in the bottle body 1 from affecting the culture effect of the Atractylodes tissue.

[0040] When the temperature and humidity detected by the first temperature and humidity sensor 102 are lower than the set value, the controller 205 controls the pump 501 to extract the external culture solution and spray it onto the Atractylodes tissue through the nozzle 5 to ensure its humidity and culture, effectively improving the effect of the Atractylodes tissue culture.

[0041] Example 2: Reference Figure 1-Figure 6 , a Atractylodes lancea tissue culture bottle, which is basically the same as Example 1, further, the inner wall of the bottle body 1 is provided with multiple first annular tubes 2 and second annular tubes 202, the first annular tube 2 is provided with an exhaust port 201, the second annular tube 202 is provided with an exhaust port 203, the first annular tube 2 is connected to the second connecting tube 211, and the second annular tube 202 is connected to the first exhaust pipe 207.

[0042] The exhaust ports 201 are equidistantly arranged on the first annular tube 2 circumferentially, and the suction ports 203 are equidistantly arranged on the second annular tube 202 circumferentially.

[0043] The first annular tube 20 is arranged on the upper side of the nozzle 5 , and the second annular tube 202 is arranged on the lower side of the nozzle 5 .

[0044] When the gas in the bottle body 1 is extracted, the gas in the bottle body 1 evenly enters the second annular tube 202 from multiple suction ports 203, and then enters the air pump 206, thereby effectively preventing the excessive air flow velocity in the bottle body 1 from affecting the culture of the Atractylodes tissue.

[0045] The exhaust port 201 is arranged on the upper side, and the suction port 203 is arranged on the lower side, so that the gas in the bottle body 1 flows from top to bottom, and the culture liquid sprayed from the nozzle 5 flows in the downward direction, so as to facilitate the culture liquid to quickly contact with the Atractylodes tissue and can more quickly increase the humidity in the bottle body 1.

[0046] Example 3: Reference Figure 1-Figure 6 , a Atractylodes lancea tissue culture bottle, which is basically the same as Example 1, and further, a collecting plate 106 is fixedly connected to the lower side of the bottle body 1, a storage cavity 109 is arranged between the lower side of the collecting plate 106 and the bottle body 1, a fixed tube 107 is arranged in the middle of the collecting plate 106, and a one-way valve is arranged in the fixed tube 107.

[0047] When the culture solution sprayed by the nozzle 5 is excessive, part of the nutrient solution passes through the culture layer 101 and falls onto the collecting plate 106 , and then falls into the storage cavity 109 through the fixed tube 107 , and a sealing plug is provided on one side of the storage cavity 109 .

[0048] Example 4: Reference Figure 1-Figure 6, a tissue culture bottle of Atractylodes lancea, which is basically the same as that of Example 1, and further, an impeller box 4 is fixedly connected to the lower side of the mounting plate 204, and two cavities 401 are arranged in the impeller box 4, and a partition 407 is fixedly connected between the two cavities 401, a mesh plate 408 is fixedly connected in one of the cavities 401, and a water-absorbing sponge 409 is fixedly connected to the mesh plate 408, and a rotating shaft 405 is rotatably arranged in the other cavity 401, and the rotating shaft 405 passes through the partition 407. 7 and a mesh plate 408, an impeller group 406 is arranged at one end of the rotating shaft 405 away from the mesh plate 408, the cavity with the impeller group 406 is communicated with the first exhaust pipe 207, a third connecting pipe 402 is fixedly connected between the two cavities 401, a pressing plate 410 for squeezing the water-absorbing sponge 409 is fixedly connected at one end of the rotating shaft 405 close to the mesh plate 408, and a second exhaust pipe 403 is fixedly connected between the cavity 401 close to the pressing plate 410 and the second annular tube 202.

[0049] A return pipe 404 is provided between the cavity 401 close to the mesh plate 408 and the storage cavity 109 .

[0050] When the air pump 206 extracts the gas in the bottle body 1, the gas enters a cavity 401 from the second air extraction pipe 403, then enters another cavity 401 after being filtered through the water-absorbing sponge 409, and finally enters the air pump 206 through the first air extraction pipe 207. The gas passing through drives the rotating shaft 405 to rotate through the impeller group 406, and the rotating shaft 405 drives the pressure plate 410 to rotate, squeezing out part of the water in the water-absorbing sponge 409, and then the culture fluid enters the storage chamber 109 along the reflux pipe 404, effectively preventing the water mist sprayed from the nozzle 5 from being extracted and circulated by the air pump 206, thereby effectively ensuring the temperature and humidity in the bottle body 1, and then effectively improving the culture effect of the Atractylodes tissue.

[0051] Example 5: A method for using an Atractylodes lancea tissue culture bottle, mainly comprising the following steps:

[0052] Step 1: Place the Atractylodes tissue on the culture layer 101 and spray an appropriate amount of culture solution;

[0053] Step 2, cover with threaded cover 108 for cultivation;

[0054] Step 3: extract the gas in the bottle 1 through the air pump 206, and then transport it into the bottle 1 to make the gas inside flow;

[0055] Step 4: Detect the temperature and humidity of the gas in the detection box 3 through the second temperature and humidity sensor 301, and then control the adjustment box 209 to start through the controller 205 to adjust the temperature and humidity of the gas.

[0056] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A tissue culture bottle of Atractylodes lancea, comprising: The bottle body (1) is characterized by further comprising: A culture layer (101) is arranged in the bottle body (1); A threaded cap (108) threadably connected to the bottle body (1); A mounting plate (204) fixedly arranged on one side of the bottle body (1); A controller (205) is fixedly mounted on the mounting plate (204); An air pump (206) is fixedly mounted on the mounting plate (204); A first air extraction pipe (207) is fixedly arranged at the air extraction end of the air pump (206), and the first air extraction pipe (207) is connected to the bottle body (1); A regulating box (209) is fixedly mounted on the mounting plate (204), and the regulating box (209) is used to regulate the temperature and humidity of the gas flowing through the regulating box; A first exhaust pipe (208) is fixedly arranged between the input end of the regulating box (209) and the output end of the air pump (206); A detection box (3) is arranged on the upper side of the adjustment box (209), and a first connecting pipe (210) is arranged between the adjustment box (209) and the detection box (3); A second temperature and humidity sensor (301) is arranged in the detection box (3); The second connecting tube (211) is arranged between the detection box (3) and the bottle body (1), and the second connecting tube (211) is in communication with the bottle body (1).

2. The Atractylodes lancea tissue culture bottle according to claim 1, characterized in that: A first temperature and humidity sensor (102) is arranged on the culture layer (101), a pump (501) is arranged on one side of the bottle body (1), an output end of the pump (501) is fixedly connected to a water spray pipe (502), a nozzle (5) is fixedly connected to the water spray pipe (502), and the nozzle (5) is arranged in the bottle body (1).

3. A kind of Atractylodes lancea tissue culture bottle according to claim 2, characterized in that, The inner wall of the bottle body (1) is provided with a plurality of first annular tubes (2) and second annular tubes (202); the first annular tube (2) is provided with an exhaust port (201); the second annular tube (202) is provided with an exhaust port (203); the first annular tube (2) is connected to the second connecting tube (211); and the second annular tube (202) is connected to the first exhaust pipe (207).

4. A tissue culture bottle of Atractylodes lancea according to claim 3, characterized in that: The exhaust ports (201) are circumferentially equidistantly arranged on the first annular tube (2), and the suction ports (203) are circumferentially equidistantly arranged on the second annular tube (202).

5. The Atractylodes lancea tissue culture bottle according to claim 3, characterized in that: The first annular tube (2) is arranged on the upper side of the nozzle (5), and the second annular tube (202) is arranged on the lower side of the nozzle (5).

6. The Atractylodes lancea tissue culture bottle according to claim 1, characterized in that: A limit ring (103) is fixedly connected inside the bottle body (1), the limit ring (103) matches the culture layer (101), a first electromagnet (104) is fixedly connected to the lower side of the culture layer (101), and a second electromagnet (105) corresponding to the first electromagnet (104) is arranged inside the bottle body (1).

7. The Atractylodes lancea tissue culture bottle according to claim 1, characterized in that: The lower side of the bottle body (1) is fixedly connected to a collecting plate (106), a storage cavity (109) is provided between the lower side of the collecting plate (106) and the bottle body (1), a fixed tube (107) is provided in the middle of the collecting plate (106), and a one-way valve is provided in the fixed tube (107).

8. The Atractylodes lancea tissue culture bottle according to claim 7, characterized in that: The lower side of the mounting plate (204) is fixedly connected to an impeller box (4), and two cavities (401) are arranged in the impeller box (4), and a partition plate (407) is fixedly connected between the two cavities (401), and a mesh plate (408) is fixedly connected to one of the cavities (401), and a water-absorbing sponge (409) is fixedly connected to the mesh plate (408), and a rotating shaft (405) is rotatably arranged in the other cavity (401), and the rotating shaft (405) passes through the partition plate (407) and the mesh plate (408), and the rotating shaft (405) passes through the partition plate (407) and the mesh plate (408). An impeller group (406) is provided at one end of the shaft (405) away from the mesh plate (408); the cavity with the impeller group (406) is connected to the first exhaust pipe (207); a third connecting pipe (402) is fixedly connected between the two cavities (401); a pressing plate (410) for squeezing the water-absorbing sponge (409) is fixedly connected to one end of the rotating shaft (405) close to the mesh plate (408); and a second exhaust pipe (403) is fixedly connected between the cavity (401) close to the pressing plate (410) and the second annular tube (202).

9. The Atractylodes lancea tissue culture bottle according to claim 8, characterized in that: A return pipe (404) is provided between the cavity (401) close to the mesh plate (408) and the storage cavity (109).

10. A method for using an Atractylodes lancea tissue culture bottle, used for the Atractylodes lancea tissue culture bottle according to claim 1, characterized in that: The main steps include: Step 1: placing the Atractylodes tissue on the culture layer (101) and spraying an appropriate amount of culture solution; Step 2, cover with a threaded cap (108) for culturing; Step 3: extracting the gas in the bottle (1) through the air pump (206), and then transporting it into the bottle (1) to make the gas inside flow; Step 4: Detect the temperature and humidity of the gas in the detection box (3) through the second temperature and humidity sensor (301), and then control the adjustment box (209) to start through the controller (205) to adjust the temperature and humidity of the gas.

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