Asiatic corn borer sex pheromone lure, preparation method and insect trap

Abstract

The invention relates to the technical field of insect trapping, and provides an Asiatic corn borer sex pheromone lure, a preparation method and an insect trap, the lure comprises an Asiatic corn borer sex pheromone, and the Asiatic corn borer sex pheromone is composed of cis-12-tetradecenol acetate (CAS35153-20-9), trans-12-tetradecenol acetate (CAS35153-21-0) and n-tetradecanol (CAS112-72-1); the content of the cis-12-tetradecenol acetate is 60 to 90 parts per lure, the content of the trans-12-tetradecenol acetate is 0 to 40 parts per lure, and the content of the n-tetradecanol is 0.01 to 10 parts per lure. The sex pheromone lure is matched with an insect trap, the Asiatic corn borer trapping efficiency can be greatly improved, the effect of stably preventing and treating pests in a planting area is achieved, and the sex pheromone lure is safe to people and livestock, free of pollution to the environment, capable of effectively reducing use of pesticides, low in use cost, convenient to operate and suitable for popularization and application. The method has stable Asiatic corn borer prediction, forecast and control effects and good social, economic and ecological benefits.

Description

Technical Field

[0001] The invention relates to the technical field of insect trapping, and in particular to an Asian corn borer sex pheromone lure core and a preparation method thereof, and an insect trap. Background Art

[0002] The Asian corn borer is an important pest in corn planting. It belongs to the genus Ostrinia of the family Pyralidae in the order Lepidoptera. It is commonly known as the corn borer. The adult is a small to medium-sized yellow moth. The male is thin and dark in color, while the female is thick and light in color. It harms all parts of the corn plant above the ground, causing the damaged part to lose function and reduce grain yield. It poses a serious threat to the yield and quality of corn and is one of the pests that need to be focused on in agricultural production. The newly hatched larvae can spin silk and droop, drifting with the wind and spreading to neighboring plants.

[0003] At present, according to research, although conventional pesticide control methods can control the Asian corn borer to a certain extent, the pesticides need to contact the crops when used, which is very likely to cause pesticide residues, thereby reducing the yield of the crops and affecting the stability of the planting operation.

[0004] In order to solve the above problems, the present application proposes an Asian corn borer sex pheromone lure and an insect trap thereof. Summary of the invention

[0005] The invention aims to provide an Asian corn borer sex pheromone lure core, a preparation method thereof and an insect trap.

[0006] Based on the above purpose, the present invention provides an Asian corn borer sex pheromone attractant core, the attractant core comprises an Asian corn borer sex attractant and a carrier:

[0007] Preferably, the effective component is that the Asian corn borer sex attractant is composed of cis-12-tetradecenyl acetate (CAS35153-20-9), trans-12-tetradecenyl acetate (CAS35153-21-0) and n-tetradecanol (CAS112-72-1);

[0008] More preferably, the content of cis-12-tetradecenyl acetate is 60 to 90 parts per bait core;

[0009] More preferably, the content of trans-12-tetradecenyl acetate is 0 to 40 parts per lure core;

[0010] More preferably, the content of n-tetradecanol is 0.01 to 10 parts per bait core;

[0011] More preferably, the carrier of the Asian corn borer sex attractant has a carrier length of 14±2 mm, a maximum cross-sectional diameter of 100 mm, and a full net weight of 730±20 mg;

[0012] Preferably, a blank lure is soaked in n-hexane for 24 hours and then aired, a quantitative amount of the prepared pheromone solution is dripped into the bowl of the rubber stopper carrier with a pipette, and after the pheromone penetrates, a small amount of petroleum ether is added to continue the penetration. After 10 minutes, it is packed in an aluminum foil packaging bag and refrigerated to form a complete lure and carrier combination.

[0013] On the other hand, the present invention also provides a method for preparing an Asian corn borer sex pheromone lure core:

[0014] S1: In an environment with a relative humidity of 40% RH to 55% RH, the qualified raw materials are prepared according to the effective ingredient content ratio and formula requirements of the Asian corn borer sex pheromone attractant core, and the auxiliary agent is n-hexane or n-dodecane;

[0015] S2: The prepared drug solution is analyzed by Agilent8890-F ID (automatic injection) to ensure the accuracy of the preparation, and the analysis results are kept on file;

[0016] S3: Seal the remaining original drug and the prepared drug solution and store them in a refrigerator;

[0017] S4: Dilute the prepared original drug 100 times with acetone, shake well, and test it in the gas phase:

[0018] S5: GC-F ID analysis was performed to ensure that the peak time of the sample was consistent with that of the standard, and the purity of the sample was > 95%.

[0019] Finally, the present invention also provides an insect trap for use with an Asian corn borer sex pheromone lure, comprising:

[0020] triangular insect trap and base plate;

[0021] Side panels are provided on both sides of the triangular insect trap, which are unfolded outwards and pasted with sticky insect boards when the triangular insect trap is in use, so as to increase the insect catching area;

[0022] More preferably, the interior of the triangular insect trap is provided with an attractant tube for storing the attractant core, which is fixed by connecting the positioning tube above the triangular insect trap to the through holes on both sides of the triangular insect trap.

[0023] As a further optimization of the present invention, side grooves for regulating the direction of odor dispersion are provided on both sides of the triangular insect trap.

[0024] As a further optimization of the present invention, a hollow tube is provided below the positioning tube for distributing the attractant odor.

[0025] As a further optimization of the present invention, a connecting sleeve is provided at the lower end of the hollow tube to connect to the lure tube.

[0026] As a further optimization of the present invention, the triangular insect trap forms odor emission in four directions after being placed, which is used to improve the trapping efficiency in the area where Asian corn borers gather.

[0027] Beneficial effects of the present invention:

[0028] The sex attractant provided by the present invention has a significant effect on attracting the Asian corn borer, is safe for humans and animals, has no pollution to the environment, can reduce the use of pesticides, has a low cost of use, is easy to operate, can be applied to the prediction and prevention of the Asian corn borer, and has good social, economic and ecological benefits.

[0029] The present invention can distribute pheromones in four directions by adding side panels and using attractants to promote aggregation in the activity area of ​​Asian corn borers. The increase in entry channels not only improves the trapping efficiency, but also increases the use area of ​​the sticky insect board, greatly improving the capture efficiency of Asian corn borers. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0031] Figure 1 A bar chart showing the number of captured Asian corn borers in the field experiment of the present invention;

[0032] Figure 2 This is a schematic diagram of the appearance structure of the triangular trap of the present invention;

[0033] Figure 3 It is a schematic diagram of the internal structure of the triangular trap of the present invention;

[0034] Figure 4 It is a schematic diagram of the internal structure of the lure tube of the present invention;

[0035] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0036] In the figure: 1. triangular insect trap; 2. bottom plate; 3. side plate; 4. side groove; 5. lure tube; 6. positioning tube; 7. hollow tube; 8. adapter sleeve. DETAILED DESCRIPTION

[0037] The following is combined with Figure 1-4 The field biological activity test in the examples is used to illustrate the use of the Asian corn borer sex pheromone attractant and the insect trap thereof in detail, but it should be understood that the protection scope of the present invention is not limited to the specific implementation methods.

[0038] Unless otherwise specified, the reagents in the embodiments of the present invention can be purchased through commercial channels.

[0039] Example 1

[0040] This embodiment provides an Asian corn borer sex pheromone lure core, the lure core comprising an Asian corn borer sex attractant and a carrier:

[0041] The active ingredients of the Asian corn borer sex attractant are composed of cis-12-tetradecenyl acetate CAS35153-20-9, trans-12-tetradecenyl acetate CAS35153-21-0 and n-tetradecanol CAS112-72-1;

[0042] The content of cis-12-tetradecenyl acetate is 60 to 90 parts per bait core;

[0043] The content of trans-12-tetradecenyl acetate is 0 to 40 parts per lure core;

[0044] The content of n-tetradecanol is 0.01 to 10 parts per bait core;

[0045] The carrier is a natural desulfurized rubber plug with a carrier length of 14±2mm, a maximum cross-sectional diameter of 100mm, and a full net weight of 730±20mg.

[0046] Example 2

[0047] This embodiment provides a method for preparing an attractant core, which is applied to an Asian corn borer sex pheromone attractant core. The specific preparation steps are as follows:

[0048] S1: In an environment with a relative humidity of 40% RH to 55% RH, the qualified raw materials are prepared according to the effective component content ratio and formula requirements of the Asian corn borer sex attractant, and the auxiliary agent is at least one of n-hexane and n-dodecane;

[0049] S2: The prepared drug solution is analyzed by Agilent8890-F ID (automatic injection) to ensure the accuracy of the preparation, and the analysis results are kept on file;

[0050] S3: Seal the remaining original drug and the prepared drug solution and store them in a refrigerator;

[0051] S4: Dilute the prepared original drug 100 times with acetone, shake well, and test it in the gas phase:

[0052] S5: GC-F ID analysis was performed to ensure that the peak time of the sample was consistent with that of the standard, and the purity of the sample was > 95%.

[0053] Example 3

[0054] This embodiment provides an application of an Asian corn borer sex attractant carrier, which is applied to an Asian corn borer sex pheromone attractant core.

[0055] Soak the blank lure in n-hexane for 24 hours and then air-dry. Use a pipette to drip a certain amount of prepared pheromone solution into the mouth of the rubber stopper carrier bowl. After the pheromone penetrates, add a small amount of petroleum ether to continue the penetration. After 10 minutes, put it into an aluminum foil packaging bag and store it in the refrigerator.

[0056] Example 4

[0057] This embodiment provides an insect trap for use with an Asian corn borer sex pheromone lure as described above, comprising: a triangular insect trap 1 and a bottom plate 2;

[0058] Side panels 3 are provided on both sides of the triangular insect trap 1, which are unfolded outwards and pasted with sticky insect boards when the triangular insect trap 1 is used to increase the insect catching area;

[0059] The inside of the triangular insect trap 1 is provided with an attractant tube 5 for storing the attractant core, and the positioning tube 6 above the attractant tube is connected to the through holes on both sides of the triangular insect trap 1 for fixing.

[0060] Side grooves 4 for regulating the odor dispersion direction are provided on both sides of the triangular insect trap 1 .

[0061] A hollow tube 7 is provided below the positioning tube 6 for distributing the smell of the attractant.

[0062] The lower end of the hollow tube 7 is provided with an adapter sleeve 8 which is connected to the lure tube 5.

[0063] The triangular insect trap 1 forms a scent emission in four directions after being placed, and is used to improve the trapping efficiency in the area where Asian corn borers gather.

[0064] Example 5

[0065] The sex pheromone lures of Asian corn borer were used in field experiments at different times.

[0066] Different doses of sex pheromone attractants were loaded into attractant tubes, and triangular insect traps were placed 1.5 to 2 m above the ground according to the living habits of the Asian corn borer. Adjacent triangular insect traps were distributed at a distance of 15 to 45 m. Field experiments were conducted for 30 days, with data collected every three days and new sticky insect boards replaced.

[0067] In order to further illustrate the feasibility of this embodiment, the effects of field trapping experiments of Asian corn borer sex pheromone lures at different dosages are listed, and the dosage is measured in ug, as shown in Table 1:

[0068] Cis-12-Tetradecenyl Acetate (parts) Trans-12-Tetradecenyl Acetate (part) Tetradecanol (parts) Average number of traps (heads / 30 days) OA1 63 6 0.05 278.41±6.35 0A2 74 11 1.81 280.06±5.87 OA3 77 11 3.93 384.25±9.78 OA4 85 25 5.57 306.46±6.45 OA5 60 30 5.62 298.27±7.15 OA6 60 33 7.17 301.29±1.77 OA7 90 35 4.28 431.44±3.57 OA8 90 38 4.34 420.41±6.74 OA9 87 40 10 465.42±6.49 OA10 87 40 9.22 470.25±2.51 OA11 69 19 6.87 330.05±8.91 OA12 71 0 3.46 350.18±6.38 OA13 84 0 3.35 411.36±7.78 OA14 87 28 2.15 364.57±8.77 OA15 85 16 4.38 387.41±3.59

[0069] Table 1

[0070] Note: The average number of traps in the table is the mean ± counting error of data collected every three days within one month.

[0071] In this experiment, the trapping value of Asian corn borer was high and stable. The four-directional trapping method can increase the probability of trapping Asian corn borer and increase the storage area after the Asian corn borer is captured, so as to prevent a single sticky insect board from being full of Asian corn borer and unable to be used continuously, so as to ensure the continuous trapping effect during the use time.

[0072] In this experiment, by investigating the number of Asian corn borer pests trapped, it was found that during the period of using Asian corn borer pheromone lures, the number of Asian corn borer pests in the 100-plant area decreased by 93-97% compared with the pests in the control area, and the average trapping rate remained above 95%. The Asian corn borer pests were effectively controlled, allowing crops to remain in a stable growth state.

[0073] It can be understood that by using the sex pheromone attractant core of the present invention in combination with an insect trap, the capture efficiency of the Asian corn borer can be greatly improved, so as to achieve the effect of stably controlling pests in the planting area. The sex pheromone attractant core of the present invention is safe for humans and animals, has no pollution to the environment, can effectively reduce the use of pesticides, has a low cost of use, is easy to operate, has stable Asian corn borer prediction and control effects, and has good social, economic and ecological benefits.

[0074] In the description of the present invention, unless otherwise specified, "plurality" means two or more than two; it should be understood that the terms "opening", "upper", "lower", "thickness", "top", "middle", "length", "inside", "around" and the like indicate orientation or positional relationship, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the components or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention.

[0075] Finally, it should be noted that the above disclosure is only a specific embodiment of the present invention, but the present invention is not limited thereto, and any changes that can be conceived by those skilled in the art should fall within the protection scope of the present invention.

Claims

1. An Asian corn borer sex pheromone lure core, characterized in that: The bait core includes an Asian corn borer sex attractant and a carrier: The effective components of the Asian corn borer sex attractant are composed of cis-12-tetradecenyl acetate (CAS35153-20-9), trans-12-tetradecenyl acetate (CAS35153-21-0) and n-tetradecanol (CAS112-72-1); The content of cis-12-tetradecenyl acetate is 60 to 90 parts per lure core; The content of trans-12-tetradecenyl acetate is 0 to 40 parts per lure core; The content of n-tetradecanol is 0.01 to 10 parts per lure; The carrier of the Asian corn borer sex attractant is a natural desulfurized rubber plug with a carrier length of 14±2mm, a maximum cross-sectional diameter of 100mm, and a full net weight of 730±20mg.

2. A method for preparing an attractant core, applied to an Asian corn borer sex pheromone attractant core as claimed in claim 1, characterized in that: The specific preparation steps are as follows: S1: In an environment with a relative humidity of 40% RH to 55% RH, at least one of n-hexane and n-dodecane is used as an adjuvant, and the technical drug is prepared according to the content ratio and formula of the effective components of the Asian corn borer sex attractant; S2: The prepared drug solution is analyzed by Agilent8890-FID and the analysis results are saved; S3: Prepare the obtained drug solution and the remaining original drug and seal them in a refrigerator; S4: Dilute the prepared original drug 100 times with acetone, shake well and perform gas phase detection: S5: GC-FID analysis ensures that the peak time of the sample is consistent with that of the standard, and the purity of the sample is >95%.

3. An insect trap for use with the Asian corn borer sex pheromone lure as claimed in claim 1, characterized in that: include: A triangular insect trap (1) and a bottom plate (2); Side plates (3) are provided on both sides of the triangular insect trap (1), which are unfolded outwards and attached with sticky insect plates when the triangular insect trap (1) is in use, so as to increase the insect catching area; The triangular insect trap (1) is provided with an attractant tube (5) for storing an attractant core, and is fixed by connecting to through holes on both sides of the triangular insect trap (1) through a positioning tube (6) above the attractant tube.

4. An insect trap according to claim 3, characterized in that: Side grooves (4) for regulating the direction of odor dispersion are provided on both sides of the triangular insect trap (1).

5. The insect trap according to claim 3, characterized in that: A hollow tube (7) is provided below the positioning tube (6) for distributing the smell of the attractant.

6. An insect trap according to claim 5, characterized in that: The lower end of the hollow tube (7) is provided with an adapter sleeve (8) which is connected to the lure tube (5).

7. An insect trap according to claim 3, characterized in that: The triangular insect trap (1) emits odor in four directions after being placed, and is used to improve the trapping efficiency in areas where Asian corn borers gather.

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