Antibacterial disinfection method adopting SAEW in cooperation with thymol and product thereof

Abstract

An antibacterial disinfection product adopting SAEW in cooperation with thymol comprises a quorum sensing inhibitor A and a disinfectant B which are assembled independently and used step by step. The quorum sensing inhibitor A comprises 5-methyl-2-isopropyl phenol and deionized water which are completely dissolved in an organic solvent, and the concentration of the 5-methyl-2-isopropyl phenol in the quorum sensing inhibitor A is not less than 256 mu g / mL; the disinfectant B comprises subacid electrolyzed water with the available chlorine concentration of 30 ppm; filling the quorum sensing inhibitor A into a spray bottle to obtain a spray A; and filling the disinfectant B into a spray bottle to obtain a spray B. Through combined application of SAEW and a quorum sensing inhibitor thymol from plants, a novel composite disinfectant with an efficient synergistic effect is obtained. The composition can significantly reduce the risk of traditional chemical disinfectants in the aspects of bacterial drug resistance generation and pathogenicity improvement, shows good antibacterial and anti-biofilm effects, can reduce the drug resistance mutation frequency of bacteria, and is expected to become an important means for clinical and public environment anti-infection prevention and treatment strategies.

Description

Technical Field

[0001] The patent of this invention relates to the technical field of antibacterial disinfection of slightly acidic electrolyzed water, especially the antibacterial disinfection method and product using SAEW in combination with thymol. Background Art

[0002] Chemical disinfectants can be divided into nine categories according to their effective ingredients: peroxides, aldehydes, alcohols, chlorine-containing, iodine-containing, phenols, ethylene oxide, biguanides and quaternary ammonium salts. In view of the potential harm caused by the residues of traditional disinfectants to the environment, slightly acidic electrolyzed water (SAEW) has been proposed as a new type of safe and environmentally friendly disinfectant. SAEW, also known as hypochlorous acid water, is a solution produced by electrolyzing dilute electrolytes (usually containing NaCl and / or HCl) in an electrolysis chamber without a diaphragm. The pH of the SAEW solution is close to neutral (5.0-6.5), and the effective chlorine exists almost entirely in the form of hypochlorous acid molecules, which can kill various bacteria, fungi and common viruses in a short time. Compared with other commonly used disinfectants such as sodium hypochlorite and 75% ethanol, SAEW does not produce disinfectant residues, does not corrode the surface of instruments, and is non-irritating to the eyes, skin and respiratory tract. SAEW has broad application prospects in cleaning and disinfection in the fields of food, medical care, environment, and surfaces due to its broad-spectrum bactericidal, instantaneous high efficiency, low cost, and environmental safety.

[0003] However, the potential harm caused by the irrational use of chemical disinfectants has attracted people's attention. Existing studies have shown that the use of chemical disinfectants can induce the emergence of disinfectant-resistant bacteria, and some disinfectant-resistant bacteria also show cross-resistance to clinical antimicrobial drugs. In addition, chemical disinfectants can also promote the transfer of bacterial resistance genes at environmental concentrations, making the resistance situation more severe. The emergence and spread of these resistant strains seriously endanger public health and limit the choice of clinically available antimicrobial drugs.

[0004] In addition, disinfectants can also promote the virulence of pathogenic microorganisms. For example, peracetic acid can cause high levels of sublethal damage to the foodborne pathogen Listeria monocytogenes, and the bacteria in this state show upregulated expression of virulence genes and enhanced invasiveness to host cells. Chlorhexidine-induced resistant Gram-negative bacteria show upregulation of virulence factor LPS assembly protein and pilus formation protein. Studies have shown that reactive oxygen free radicals (ROS) produced during disinfectant damage may be an important means for pathogens to regulate virulence factors, that is, when ROS is reduced to non-lethal levels, it is easier to induce pathogens to produce drug-resistant mutations. Therefore, anti-virulence therapy has become a new treatment strategy for clinical combating multidrug-resistant bacterial infections.

[0005] Slightly acidic electrolyzed water (SAEW) is similar to other chlorine-containing disinfectants in that it exerts its antibacterial effect through the oxidative potential of hypochlorous acid. Its potential harm in promoting bacterial resistance and virulence will limit its application. In addition, the emergence of strains resistant to chlorine-containing disinfectants will further limit the antibacterial effect of SAEW. Summary of the invention

[0006] The purpose of the present invention is to solve the existing technical problems and provide an antibacterial disinfection method and product thereof which adopts slightly acidic electrolyzed water (SAEW) in combination with plant extract thymol (5-methyl-2-isopropylphenol) to exhibit good antibacterial and anti-biofilm effects and can reduce the frequency of drug-resistant mutations of bacteria.

[0007] The antimicrobial disinfection product using SAEW and thymol includes quorum sensing inhibitor A and disinfectant B which are independently assembled and used in steps; The quorum sensing inhibitor A comprises 5-methyl-2-isopropylphenol and deionized water completely dissolved in an organic solvent, and the concentration of 5-methyl-2-isopropylphenol in the quorum sensing inhibitor A is not less than 256 μg / mL; Disinfectant B includes slightly acidic electrolyzed water with an effective chlorine concentration of 30 ppm; Put quorum sensing inhibitor A into a spray bottle to obtain spray A; Fill disinfectant B into a spray bottle to obtain spray B.

[0008] Furthermore, the spray bottle may be a double-headed spray bottle, wherein one side of the spray bottle has a spray head for releasing spray A, and the other side of the spray bottle has a spray head for releasing spray B.

[0009] Preferably, the organic solvent in the quorum sensing inhibitor A is ethanol with a mass fraction of 2 to 5%.

[0010] Preferably, the concentration of 5-methyl-2-isopropylphenol in the quorum sensing inhibitor A is 256-1024 μg / mL.

[0011] Furthermore, the concentration of 5-methyl-2-isopropylphenol in the quorum sensing inhibitor A is preferably 512 μg / mL.

[0012] Furthermore, the quorum sensing inhibitor A also contains 0.01-0.08% by mass of edible flavoring and 0.01-0.08% by mass of edible coloring.

[0013] Preferably, the disinfectant B is prepared by a slightly acidic electrolyzed water generator and is prepared by electrolyzing NaCl and / or HCl solution in a slightly acidic electrolyzed water generator.

[0014] The antibacterial disinfection method of the product of the present invention comprises spraying spray A evenly on the area to be disinfected, and after the area is allowed to act for 10 minutes, spraying spray B evenly on the same area to be disinfected to complete the disinfection operation of the environment or medical equipment.

[0015] The antibacterial disinfection method of the product of the present invention uses the quorum sensing inhibitor A as a gargle, which stays in the oral cavity for 10-30 seconds before being spit out, the mouth is rinsed with clean water, and then the spray B is used to spray the mouth to clean the oral cavity, thereby completing an oral disinfection operation for non-therapeutic purposes.

[0016] The antibacterial disinfection method of the product of the present invention comprises using spray A to clean the oral cavity, rinsing the mouth with clean water after acting for 30-60 seconds, and then using spray B to clean the oral cavity, thereby completing an oral disinfection operation for non-therapeutic purposes.

[0017] Furthermore, for children, spray A can be evenly sprayed onto the surface of cotton wool, and disinfection can be achieved by stirring the cotton wool in the mouth. After 30-60 seconds, rinse the mouth with water, and then spray spray B evenly onto the surface of another cotton wool, repeat the stirring operation to complete the oral disinfection operation.

[0018] The preparation method of the product of the present invention comprises: 1) Preparation of spray A: add thymol (5-methyl-2-isopropylphenol) solid into ethanol, stir until completely dissolved, then add edible flavor and edible coloring aqueous solution, stir evenly, finally add the remaining amount of deionized water, stir evenly to obtain quorum sensing inhibitor A, then add quorum sensing inhibitor A into a spray bottle to obtain spray A; 2) Preparation of spray B: prepare a NaCl solution at a ratio of 1.875 g / L, use a slightly acidic electrolyzed water generator to electrolyze the NaCl solution to obtain 50 ppm SAEW, and then dilute it with water to 30 ppm to obtain disinfectant B. Add disinfectant B into a spray bottle to obtain spray B.

[0019] The beneficial effect of the present invention is that a novel composite disinfectant with high efficiency and synergistic effect is obtained by the combined use of SAEW and thymol, a quorum sensing inhibitor from plants. This combination can significantly reduce the risk of traditional chemical disinfectants in terms of bacterial resistance and pathogenicity. The use of low-concentration SAEW can show good antibacterial and anti-biofilm effects, is highly safe, and can reduce the frequency of bacterial resistance mutations. It is expected to become an important means of clinical and public environment anti-infection prevention and control strategies. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a graph showing the colony count results of an experiment in which SAEW and thymol were used to remove mature bacterial biofilms (24-well plate) in Example 2 of the present invention; Figure 2This is a graph showing the colony count results of an experiment in which SAEW cooperates with thymol to remove mature bacterial biofilm (medical catheter) in Example 2 of the present invention; Figure 3 This is a statistical result diagram of ROS fluorescence intensity of the strain after SAEW and thymol treatment in Example 3 of the present invention; Figure 4 The results of the experiment on the effect of SAEW and thymol on reducing bacterial toxicity in Example 4 of the present invention are as follows: lasA, lasB, rhlA, PcqI and PcqI Relative expression statistical results; Figure 5 This is a graph showing the effect of 512 μg / mL thymol on the frequency of drug-resistant mutations of Pseudomonas aeruginosa PAO1 after high-concentration SAEW drug-resistant treatment in Example 5 of the present invention; Figure 6 This is a photo of the results of the mouse skin acute toxicity experiment in Example 6 of the present invention; Figure 7 These are photos of the right eyes of mice in each group in the mouse eye stimulation experiment in Example 6 of the present invention. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0022] Example 1 Preparation of antibacterial disinfection products using SAEW and thymol 1) Preparation of spray A: 32, 64, 128, 256, 512, 1024 μg / mL of 5-methyl-2-isopropylphenol (Shanghai Yuanye Biotechnology Co., Ltd.) solid was added to ethanol, stirred until completely dissolved, then added with an aqueous solution of edible flavoring and edible coloring, stirred evenly, and finally added with the remaining amount of water, stirred evenly to obtain each group of quorum sensing inhibitor A, and then each group of quorum sensing inhibitor A was added to a spray bottle to obtain spray A; The quorum sensing inhibitor A also includes: 2-5% by mass of ethanol, 0.01-0.08% by mass of edible flavoring, 0.01-0.08% by mass of edible coloring, and the balance being deionized water.

[0023] 2) Preparation of spray B: prepare a NaCl solution at a ratio of 1.875 g / L, use a slightly acidic electrolyzed water generator to electrolyze the NaCl solution to obtain 50 ppm SAEW, and then dilute it to 30 ppm with purified water to obtain disinfectant B, and add disinfectant B to a spray bottle to obtain spray B; Disinfectant B includes slightly acidic electrolyzed water with an effective chlorine concentration of 30 ppm.

[0024] Example 2 Thymol combined with slightly acidic electrolyzed water (SAEW) effectively removes mature bacterial biofilm 2.1 SAEW synergistically removes mature bacterial biofilms with thymol The Pseudomonas aeruginosa model strain PAO1 (purchased from the National Health Commission Clinical Laboratory Center NCCL) was added to LB broth (OXOID, UK), and the McFarland turbidity was adjusted to 0.5 to obtain a bacterial solution. 1 mL of the bacterial solution was added to each well of a 24-well plate and placed in a CO2 incubator at 37°C for 12 hours to form a mature biofilm. Before the experiment, the mature biofilm was rinsed with PBS (Guangzhou Kerong Biological Co., Ltd.) to remove suspended bacteria.

[0025] In the experiment, the combined group first added 500 μL of quorum sensing inhibitor A containing 32, 64, 128, 256, 512, and 1024 μg / mL thymol to each well for 10 minutes; after the action, the solution was discarded, rinsed once with PBS, and then 500 μL of disinfectant B was added for 10 minutes; after the action, the solution was discarded, and a 0.5% sodium thiosulfate solution (Shanghai Aladdin Biochemical Technology Co., Ltd.) was added to remove residual chlorine, and then 1 mL PBS was added to each well and ultrasonically broken to release bacteria to obtain a bacterial suspension, which was then lysed at 10 -1 -10 -7 After gradient dilution, the culture was inoculated on LB agar plates and cultured at 37°C for 16-18 hours before colony counting.

[0026] The difference between the single-drug group and the combined group is that quorum sensing inhibitor A or disinfectant B was added only once, and after 10 minutes of action, a 0.5% sodium thiosulfate solution was added to remove residual chlorine.

[0027] The difference between the control group and the combined group is that 0.5% sodium thiosulfate solution was directly added to each well, and then 1 mL of PBS was added to each well and ultrasonically broken to release the bacteria to obtain a bacterial suspension. The bacterial suspension was then heated to 10 -1 -10 -7 After gradient dilution, the culture was inoculated on LB agar plates and cultured at 37°C for 16-18 hours before colony counting.

[0028] like Figure 1As shown, when 32, 64, and 128 μg / mL (1 / 8xMIC -1 / 8xMIC) thymol were combined with 30ppm SAEW, no combined effect was shown; when 256 μg / mL thymol was combined with SAEW, PAO1 in the biofilm was reduced by 5.03 logCFU / mL, which was significantly improved compared with the SAEW monotherapy group and the 256 μg / mL thymol monotherapy group, with statistically significant differences; when 512 μg / mL thymol was combined with SAEW, PAO1 in the biofilm was reduced by 5.73 log CFU / mL, which was close to the detection limit (10 2 CFU / mL), compared with the SAEW single-drug treatment group and the 512 μg / mL thymol single-drug treatment group, the effect was significantly improved, with statistical significance. Using 1024 μg / mL thymol alone to treat the biofilm or using SAEW for synergistic treatment reduced the amount of bacteria in the biofilm to below the detection limit, so its combined effect with SAEW could not be reflected in this embodiment. At the same time, due to the high concentration of thymol, it is very easy to precipitate, and the stability of quorum inhibitor A is not good.

[0029] The above results show that the application of thymol combined with slightly acidic electrolyzed water (SAEW) can effectively remove mature biofilms of bacteria and has good antibacterial and anti-biofilm effects. It was found that 256 μg / mL thymol combined with SAEW can effectively remove biofilms, and the combination of 512 μg / mL thymol and SAEW has the best effect. The bactericidal activity of 1024 μg / mL thymol administered alone is below the detection limit.

[0030] 2.2 SAEW and thymol remove mature biofilm on medical catheters According to the treatment method in 2.1, the 24-well plate was replaced with a 1-cm-long medical catheter (Jiangsu Suyun Medical Equipment Co., Ltd.) for biofilm culture, and single-drug and combined-drug group experiments were performed using quorum inhibitor A containing 128, 256, and 512 μg / mL thymol and 30 ppm disinfectant B, respectively.

[0031] The results are as follows Figure 2 As shown, when 128 μg / mL (1 / 2×MIC) thymol was combined with 30ppm SAEW, no combined effect was shown; when 256 μg / mL (1×MIC) and 512 μg / mL (2×MIC) thymol were combined with 30ppm SAEW, a good combined effect of removing catheter biofilm was shown; among them, 256 μg / mL thymol combined with SAEW could reduce the bacterial amount in catheter biofilm by 2.7 log CFU / mL; 512μg / mL thymol combined with SAEW could reduce the bacterial amount in catheter biofilm to below the detection limit.

[0032] Example 3 Analysis of Reactive Oxygen Species (ROS) Production in Bacteria after SAEW and Thymol Treatment The strain was detected using a reactive oxygen species (ROS) detection kit (Shanghai Biotech Co., Ltd.). PAO1 was cultured in LB broth overnight with shaking to obtain a bacterial solution. 8 mL of the bacterial solution was centrifuged at 4000 rpm for 5 minutes, the supernatant was discarded, and the solution was washed twice with PBS. Finally, the turbidity of the bacterial solution was adjusted to OD600 of 0.3-0.4. Then, 12 μL of the probe was mixed with 12 mL of the bacterial solution (1:1000 dilution of the probe), placed in a 15 mL centrifuge tube, wrapped in tin foil to avoid light, and incubated at 37°C for 20 minutes. The mixture was mixed by inversion every 3-5 minutes to ensure full contact between the probe and the bacterial solution. After the bacterial solution and the probe were mixed, they were divided into 10 1.5 mL centrifuge tubes, with 0.9 mL in each tube. After centrifugation at 4000 rpm for 5 minutes, the tubes were washed twice with PBS to remove the free probe and obtain the experimental bacterial solution loaded with the probe.

[0033] Perform the following operations on the experimental bacterial solution: The single-drug treatment groups were treated according to the following schemes: 1) after centrifuging the bacteria at 4000 rpm for 5 minutes, 1 mL of 30 ppm SAEW was added to treat the bacteria for 10 minutes; or 2) after centrifuging the bacteria at 4000 rpm for 5 minutes, 1 mL of 128, 256, or 512 μg / mL thymol was added to treat the bacteria for 10 minutes.

[0034] The combined group was treated as follows: first, 1 mL of 128, 256, or 512 μg / mL thymol was added to treat the cells for 10 min, the cells were centrifuged at 4000 rpm for 5 min, the supernatant was discarded, and then the cells were treated with 30 ppm SAEW for 10 min.

[0035] The positive control group used the control reagent Rosup provided by the reactive oxygen species (ROS) detection kit, diluted 1:1000 by volume, added 1 mL to the mixed bacterial solution, and treated for 10 minutes; the negative control group used 1 mL of pure water for 10 minutes.

[0036] After the treatment, the plate was plated for detection, 200 μL per well, and each group was repeated 3 wells. The excitation wavelength was 488 nm, the emission wavelength was 525 nm, and the gain was 100 / 80. The intensity of fluorescence before and after stimulation was detected, and the data was recorded and analyzed. Figure 3As shown, compared with the control group, the ROS in the 30 ppm SAEW group was significantly increased; the ROS in the 128, 256 and 512 μg / mL thymol single-drug groups increased in a dose-dependent manner; compared with their respective single-drug groups, the ROS levels in each combination group were increased, among which the ROS level in the 256 μg / mL thymol and 30ppm SAEW combination group was significantly increased compared with the 30ppm SAEW group and the 256 μg / mL thymol group; when 512 μg / mL thymol was combined with SAEW, the data statistics showed that the increase in ROS was not as good as that in the single-drug group, because a better bactericidal effect had been achieved in the combined case, resulting in the leakage and loss of the fluorescent probe in the cells.

[0037] Example 4 Experiment on reducing bacterial toxicity by SAEW and thymol RT-qPCR was used to study the expression changes of bacterial virulence genes under different drug combinations. The present invention selected five known key virulence genes of PAO1 for analysis, including lasA, lasB, rhlA, pqsA and PcqI RT-qPCR experiments mainly include three steps: RNA extraction, RNA reverse transcription into cDNA and cDNA qPCR. The research methods used in the present invention are all classic RT-qPCR steps.

[0038] First, the treatments were carried out according to the following groups: (1) control group: no treatment; (2) single drug group: treated with 30 ppm SAEW and 512 μg / mL thymol, respectively; (3) combination group: first treated with 512 μg / mL thymol, then treated with 30 ppm SAEW after a 10-minute interval. After treatment, the biofilm was broken to release the bacteria in the biofilm and cultured in LB medium at 37°C to obtain a sufficient amount of bacteria (1×10 8 CFU / mL) for RNA extraction.

[0039] rpoB was used as the housekeeping gene and 2 -ΔΔCt The expression levels of virulence genes were calculated by the RNA extraction kit (Bao Ri Yi Biotechnology (Beijing) Co., Ltd.), and cDNA was synthesized using the Bacterial RNA Miniprep Kit (Bao Ri Yi Biotechnology (Beijing) Co., Ltd.) and the PrimeScript™ II 1st Strand cDNA Synthesis Kit (Bao Ri Yi Biotechnology (Beijing) Co., Ltd.). TB Green ® PremixEx Taq™ PCR amplification was performed with Tli-RNaseHP1μs. The sequences of the amplification primers are shown in Table 1.

[0040] Table 1. Primer sequences Pseudomonas aeruginosa PAO1 is able to degrade elastin (a major component of connective tissue), which may be an important reason for its pathogenicity and its proliferation in human tissues. LasA is an extracellular enzyme required for its elastase activity. Extracellular secreted LasB is considered to be an important virulence factor of Pseudomonas aeruginosa. It is also involved in the processing of LasA, thereby enhancing elastase activity. Figure 4 As shown, compared with the control group and the single drug group, the combination group can significantly reduce the aeruginosa virulence gene alas , alasB The relative expression of alas ;right rhA , PcqI , PcqI The above results show that the combined group can reduce lasA、lasB It has good application prospects in the field of induced bacterial virulence.

[0041] Example 5 Thymol reduces the frequency of drug-resistant mutations of Pseudomonas aeruginosa PAO1 to SAEW Two high concentrations of SAEW, 60 ppm and 80 ppm, were selected as experimental reagents to simulate the formation of drug-resistant bacteria induced by high concentration disinfectants.

[0042] A single PAO1 pure colony was picked and cultured in 20 mL MH broth (OXOID, UK) with shaking for 18-24 h. After centrifugation at 3000 rpm for 10 min, the precipitate was resuspended and added to 200 mL MH broth and cultured with shaking at 200 rpm for 6 h. The culture was centrifuged again at 3000 rpm for 10 min, the supernatant was discarded, and the bacterial solution concentration was adjusted to 1.0 × 10 9 CFU / mL, and MH solid medium containing 512 μg / mL thymol was prepared for use. First, the bacteria were treated with 60 ppm and 80 ppm SAEW disinfectants for 10 minutes, and then the mixed suspension containing SAEW and bacteria was spread on blank MH solid culture medium and drug-containing MH solid culture medium respectively. The SAEW reaction was not terminated here to simulate the inhibitory effect of the two on the frequency of drug-resistant mutations under the combined action, and the coated plates were incubated at 37°C for 3 days.

[0043] The colony count results are as follows Figure 5 The results showed that 512 μg / mL thymol could significantly reduce the PAO1 resistance mutation rate caused by SAEW, and the frequency of resistance mutation could be reduced by 1×10 3 The combined use of thymol and SAEW can reduce the frequency of bacterial resistance mutations and is less likely to induce the formation of resistant bacteria.

[0044] Example 6 Mouse skin acute toxicity test and mouse eyeball irritation test With reference to the Technical Specifications for Safety of Cosmetics (2015 edition), a single limit method experiment was adopted.

[0045] The application area was 10% of the mouse's body surface area, and the hair removal range was 3cm×3cm. The drugs were evenly applied to the shaved area on the back of the mice according to the control group (1 mL of deionized water), 1 mL of 512μg / mL thymol, 1 mL of 30ppm SAEW, and the combination group (first apply 1 mL of 512μg / mL thymol for 10 minutes and then apply 1 mL of 30 ppm SAEW). The experimental mice were observed at 1 hour, 3 hours, 8 hours and 24 hours after administration. There was no obvious change in the general living conditions of the mice, and their breathing, limbs, behavioral activities, etc. were all normal. Figure 6 As shown, in the acute toxicity experiment on mouse skin, compared with the control group, no redness, damage or erythema formation was observed on the mouse skin in the single-drug group and the combination group within 1-24 hours after treatment, proving that the combined use of SAEW and thymol does not cause skin irritation.

[0046] Mouse eyeball irritation experiment: The experimental animals were 6 SPF mice in each group (half male and half female, females were not pregnant and had never given birth), for a total of 4 groups. After 3 days of adaptive feeding, the mouse eyeball irritation experiment was performed.

[0047] During the experiment, the mouse eyeballs were gently opened, and the control group (10 μL of deionized water), 10 μL of 512 μg / mL thymol, 10 μL of 30ppm SAEW, and the combined group (10 μL of 512 μg / mL thymol was first dripped for 10 minutes and then 10 μL of 30ppm SAEW) were dripped into the right eyeball of the mouse, so that the mouse eyes were passively closed for 1 second to prevent the loss of the test substance. 10 μL of deionized water was dripped into the left eye of each group. The eyes were not rinsed within 24 hours after the test substance was dripped. The eyes of the mice were observed and photographed 1 hour, 24 hours, and 48 hours after the test substance was dripped. The observation showed that the state of the left eyeball of the mice in each group was similar to that of the right eyeball of the control group.

[0048] like Figure 7 As shown, in the mouse eye irritation experiment, compared with the control group, no redness, swelling, inflammation, turbidity, or tearing was observed in the eyes of the 30 ppm SAEW, 512 μg / mL thymol, and combination groups within 1-48 hours after treatment, which proves that the combined application of SAEW and thymol is not irritating to the eyes.

[0049] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. The antibacterial disinfection product using SAEW and thymol is characterized by: It includes a quorum sensing inhibitor A and a disinfectant B that are independently assembled and used in steps; The quorum sensing inhibitor A comprises 5-methyl-2-isopropylphenol and deionized water completely dissolved in an organic solvent, and the concentration of 5-methyl-2-isopropylphenol in the quorum sensing inhibitor A is not less than 256 μg / mL; Disinfectant B includes slightly acidic electrolyzed water with an effective chlorine concentration of 30 ppm; Put quorum sensing inhibitor A into a spray bottle to obtain spray A; Fill disinfectant B into a spray bottle to obtain spray B.

2. The antibacterial disinfection product according to claim 1, characterized in that: The organic solvent in the quorum sensing inhibitor A is ethanol with a mass fraction of 2 to 5%.

3. The antibacterial disinfection product according to claim 1, characterized in that: The concentration of 5-methyl-2-isopropylphenol in quorum sensing inhibitor A was 256-1024 μg / mL.

4. The antibacterial disinfection product according to claim 3, characterized in that: Disinfectant B is prepared by using a slightly acidic electrolyzed water generator.

5. The antibacterial disinfection method of the product according to claims 1-4, characterized in that: Spray A evenly onto the area to be disinfected. After 10 minutes, spray B evenly onto the same area to be disinfected to complete the disinfection of the environment or medical equipment.

6. The antibacterial disinfection method of the product according to claims 1-4, characterized in that: Use quorum sensing inhibitor A as a gargle, keep it in the mouth for 10-30 seconds before spitting it out, rinse the mouth with clean water, and then use spray B to spray the mouth to clean the mouth, completing the oral disinfection operation for non-therapeutic purposes.

7. The antibacterial disinfection method of the product according to claims 1-4, characterized in that: Use spray A to clean the oral cavity, rinse the mouth with water after 30-60 seconds, and then use spray B to clean the oral cavity to complete the oral disinfection operation for non-therapeutic purposes.

Images