Application of sericin liquid in improving salt tolerance of plants and sericin plant foliage spraying agent

Abstract

The invention discloses application of sericin liquid in improving salt tolerance of plants and a sericin plant leaf surface spraying agent, and relates to the technical field of pesticide production. The method can be used for improving the salt tolerance of plants and developing the application of the silk protein in agriculture, especially the application of the silk protein in the soil salinization problem, a new thought and a new method are possibly provided for solving the soil salinization problem, a new field is possibly developed for the application of the silk protein, and new opportunities and challenges are brought to agricultural production.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticide production, and more specifically to an application of sericin liquid in improving plant salt tolerance and a sericin plant foliar spray. Background Art

[0002] Soil salinization has become one of the important stresses faced in agricultural production. There are many reasons for soil salinization, including low rainfall, weathering of native rocks, inappropriate irrigation and fertilization during farming, etc. High-salt soil causes ion poisoning, osmotic stress, and oxidative stress, making it difficult for roots to absorb water, disrupting the normal physiological metabolism of plants, thereby hindering plant growth and development, and seriously affecting crop yields in agricultural production. How to improve plant salt tolerance and cultivate new salt-tolerant varieties has become an important research goal and an urgent problem to be solved in the development and utilization of salinized land.

[0003] Silk is a natural high-molecular protein fiber and an important textile material. It does not require a large amount of chemicals during the production process, so it is more environmentally friendly. In addition, the biodegradability of silk protein means that it can be quickly decomposed in the soil, reducing the impact on the environment. With the in-depth study of the characteristics of silk, the application value of silk has been expanded in many fields. Silk protein mainly includes two components, namely sericin and fibroin. Sericin and fibroin are used in cosmetics, food, and biomedical materials due to their excellent biocompatibility, morphological plasticity, moisturizing and antioxidant functions. Among them, sericin has also been developed and utilized in cosmetics production due to its good moisturizing and other functions, and it is necessary to develop the application of sericin in agriculture. Summary of the invention

[0004] In order to solve the above problems, the present invention provides an application of sericin solution in improving the salt tolerance of plants and a sericin plant foliar spray, which are used to improve the salt tolerance of plants.

[0005] The present invention is achieved through the following technical solutions:

[0006] A first aspect of the present invention provides use of a sericin solution in improving salt tolerance of plants, wherein the concentration of the sericin solution is 0.1 g / L to 1 g / L.

[0007] Preferably, the concentration of the sericin solution is 0.2 g / L.

[0008] Preferably, the sericin solution is sprayed on the front side of the leaves, with a spraying amount of 1 mL to 2 mL per plant each time.

[0009] Preferably, the improvement of plant salt tolerance is manifested as an increase in plant height, stem thickness and biomass under a salt stress environment.

[0010] Preferably, the salt stress environment refers to a salt stress environment with a sodium chloride concentration of 100 mM to 500 mM.

[0011] Preferably, the plant comprises tomato.

[0012] Preferably, the sericin liquid is prepared by taking silkworm cocoons, placing them in water and boiling them to dissolve the sericin, and removing undissolved silk to obtain the sericin liquid.

[0013] The ratio of the clean silkworm cocoons to water is 5 g to 10 g: 200 mL.

[0014] The second aspect of the present invention provides a sericin plant foliar spray, which is the sericin liquid.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention prepares a sericin solution from silk protein, wherein the concentration of the sericin solution is 0.1 g / L to 1 g / L. Spraying the sericin solution on the front of the leaves can improve the salt tolerance of the plant and increase the growth of the plant under salt stress, which is manifested in the increase of plant height, stem thickness and biomass. Therefore, the sericin solution can be used as a new spraying agent to improve the salt tolerance of plants and to explore the application of silk protein in agriculture, especially in the problem of soil salinization. It is not only possible to provide new ideas and methods for solving the problem of soil salinization, but also possible to open up new fields for the application of silk protein, and bring new opportunities and challenges to energy-saving and environmentally friendly agricultural production. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 The growth of plants sprayed with distilled water and sericin solution on the front of leaves.

[0019] Among them, A is spraying distilled water on the front side of the leaves, HS1, and the three pots are three parallel tests; B is spraying sericin solution on the front side of the leaves, DS, and the three pots are three parallel tests.

[0020] Figure 2 Measurement and analysis of various growth indicators of plants sprayed with sericin solution and distilled water on the front of leaves.

[0021] Among them, A is the relative growth rate of plant height of plants sprayed with sericin solution on the front of leaves and distilled water on the front of leaves, B is the salt tolerance index of plant height of plants sprayed with sericin solution on the front of leaves and distilled water on the front of leaves, C is the relative growth rate of stem diameter of plants sprayed with sericin solution on the front of leaves and distilled water on the front of leaves, D is the salt tolerance index of stem diameter of plants sprayed with sericin solution on the front of leaves and distilled water on the front of leaves, E is the root fresh weight of plants sprayed with sericin solution on the front of leaves and distilled water on the front of leaves, F is the relative reduction rate of biomass calculated by root fresh weight of plants sprayed with sericin solution on the front of leaves and distilled water on the front of leaves, G is the relative reduction rate of biomass calculated by root fresh weight of plants sprayed with sericin solution on the front of leaves and distilled water on the front of leaves The root dry weight of the plants sprayed with sericin liquid and distilled water on the front side of the leaves, H is the relative reduction rate of biomass calculated as root dry weight of the plants sprayed with sericin liquid and distilled water on the front side of the leaves, I is the aboveground fresh weight of the plants sprayed with sericin liquid and distilled water on the front side of the leaves, J is the relative reduction rate of biomass calculated as aboveground fresh weight of the plants sprayed with sericin liquid and distilled water on the front side of the leaves, K is the aboveground dry weight of the plants sprayed with sericin liquid and distilled water on the front side of the leaves, and L is the relative reduction rate of biomass calculated as aboveground dry weight of the plants sprayed with sericin liquid and distilled water on the front side of the leaves.

[0022] Figure 3 The growth of plants sprayed with distilled water and sericin solution on the back of leaves.

[0023] Among them, A is spraying distilled water on the back of the leaves, HS2, and the three pots are three parallel tests; B is spraying sericin solution on the back of the leaves, BS, and the three pots are three parallel tests.

[0024] Figure 4 Measurement and analysis of various growth indicators of plants sprayed with sericin solution and distilled water on the back of leaves.

[0025] Among them, A is the relative growth rate of plant height of plants sprayed with sericin solution and distilled water on the back of leaves, B is the salt tolerance index of plant height of plants sprayed with sericin solution and distilled water on the back of leaves, C is the relative growth rate of stem diameter of plants sprayed with sericin solution and distilled water on the back of leaves, D is the salt tolerance index of stem diameter of plants sprayed with sericin solution and distilled water on the back of leaves, E is the root fresh weight of plants sprayed with sericin solution and distilled water on the back of leaves, F is the relative reduction rate of biomass calculated by root fresh weight of plants sprayed with sericin solution and distilled water on the back of leaves, G is the relative reduction rate of biomass calculated by root fresh weight of plants sprayed with sericin solution and distilled water on the back of leaves, The root dry weight of the plants sprayed with sericin liquid and distilled water on the back of the leaves, H is the relative reduction rate of biomass calculated as root dry weight of the plants sprayed with sericin liquid on the back of the leaves and distilled water on the front and back of the leaves, I is the aboveground fresh weight of the plants sprayed with sericin liquid on the back of the leaves and distilled water on the back of the leaves, J is the relative reduction rate of biomass calculated as aboveground fresh weight of the plants sprayed with sericin liquid on the back of the leaves and distilled water on the back of the leaves, K is the aboveground dry weight of the plants sprayed with sericin liquid on the back of the leaves and distilled water on the back of the leaves, and L is the relative reduction rate of biomass calculated as aboveground dry weight of the plants sprayed with sericin liquid on the back of the leaves and distilled water on the back of the leaves. DETAILED DESCRIPTION

[0026] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively below, and preferred embodiments of the present invention are provided. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0028] The inventive concept of the present invention is as follows:

[0029] Sericin is a pure natural protein that has been used in the fields of cosmetics and biomedicine. Considering that solutions or materials prepared from sericin have good biocompatibility, moisturizing properties, and antioxidant effects, it is expected that sericin solutions used as agricultural sprays may help alleviate the adaptability of plants to adverse environmental stresses. However, there is currently no research on the use of sericin in improving crop resistance.

[0030] Based on this, the present invention provides the use of sericin solution in improving the salt tolerance of plants, wherein the concentration of the sericin solution is 0.1 g / L to 1 g / L. This concentration range can ensure that the sericin solution can be effectively absorbed by the plant and exert its effect after being sprayed on the plant leaves, thereby improving the salt tolerance of the plant.

[0031] Based on the same inventive concept, the present invention provides a sericin plant foliar spray, which is the sericin liquid. The spray can improve the salt tolerance of plants and increase the growth of plants under salt stress, which is specifically manifested in the increase of plant height, stem thickness, and biomass. Through the implementation of the present invention, sericin is not only used in the fields of cosmetics and biomedicine, but its new use as an agricultural spray component has also been expanded, providing a new type of spray component for the development of agricultural sprays.

[0032] In order to better illustrate the present invention, the present invention is further described below in conjunction with the embodiments. The following are specific embodiments.

[0033] Example 1

[0034] The method for preparing sericin solution includes: taking silk cocoons, removing the outermost and innermost silk fibers, taking the clean middle part as the clean silk cocoons for preparing sericin solution. Cut 5g of the clean silk cocoons into pieces, place them in 200mL of distilled water and boil them for 10 minutes; remove the remaining undissolved silk fibers, and the resulting solution is the sericin solution.

[0035] Example 2

[0036] The method for preparing sericin solution includes: taking silk cocoons, removing the outermost and innermost silk fibers, taking the clean middle part as the clean silk cocoons for preparing sericin solution. Cut 10g of clean silk cocoons into pieces, place them in 200mL of distilled water and boil for 10 minutes; remove the remaining undissolved silk fibers, and the resulting solution is the sericin solution.

[0037] Experiment 1

[0038] The ability of spraying sericin solution on the front side of leaves to improve the salt tolerance of plants was experimentally verified.

[0039] (I) Preparation of experimental materials

[0040] The concentration of the sericin solution was detected by the BCA method and diluted to 0.2 g / L with distilled water for subsequent experiments.

[0041] The plant material for the test was tomato, the variety was Ailsa craig. The tomato seeds were disinfected with 70% alcohol for 30 seconds, 10% sodium hypochlorite for 10 minutes, rinsed with sterile water 5 times, dried with sterile filter paper, and placed in a dark culture at 28°C until germination. The germinated tomato seeds were transplanted into pots and placed in a light culture box. The light was divided into two stages: stage one was 22000xl, 25°C, 16h, and stage two was dark without light, 18°C, 8h culture. The experiment began when the tomato grew to the stage of three leaves and one heart.

[0042] 2. Experimental design

[0043] Tomato plants with the same growth and size were selected for experimental treatment. Two groups of experimental plants were set up, with at least 10 plants in each group: Group A was the distilled water group, with distilled water sprayed on the front of the leaves, marked as HS1; Group B was the sericin group, with sericin solution sprayed on the front of the leaves, marked as DS.

[0044] The spraying steps are as follows: place distilled water or sericin solution in a 200 mL spray bottle, and spray the front side of the leaves at the time when darkness turns to light in the light incubator, spraying 1.5 mL each time to ensure that the leaf surface is evenly sprayed.

[0045] The plant height and stem diameter data of the tested plants were recorded before treatment, and 350mM NaCl solution was irrigated every 3 days for 18 consecutive days, and tracking and photographing were taken during the spraying experiment. After the treatment, the plant height and stem diameter data were recorded, and the roots and aerial parts were separated and weighed for fresh weight respectively. After drying at 85℃ for 5 days, the dry weight was weighed.

[0046] In addition, two groups of experimental plants were set up as controls without salt treatment. Other treatments and observation steps were the same as those of groups A and B, which served as references for calculating the salt tolerance index and the relative reduction rate of biomass.

[0047] The experimental data obtained are calculated according to the following formula:

[0048] Relative Growth Rate (RGR) = (Y t2 -Y t1 ) / (t2-t1).

[0049] Salt Tolerance Index (STI) = (Y 盐t2 -Y 盐t1 ) / (Y 对照t2 -Y 对照t1 ).

[0050] Relative Decrease in Plant Biomass (RDPB) = (Y 对照t2 -Y 盐t2 ) / Y 对照t2 .

[0051] In the above formula, Y is the measured value of plant height, stem diameter, fresh weight or dry weight; t1 and t2 are the start and end time points of the experiment, that is, t2-t1 is the treatment time, and the time unit is day; salt and control are the values ​​under salt treatment and no salt treatment, respectively.

[0052] (III) Analysis of experimental results

[0053] This experimental case was treated with 350 mM NaCl solution for 18 days. Figure 1 The growth of plants sprayed with distilled water and sericin solution on the front of leaves, Figure 1 Middle A: Spraying distilled water on the upper side of leaves, HS1; Figure 1 Middle B shows the front side of leaves sprayed with sericin solution, DS. Figure 2 For the measurement and analysis of various growth indicators.

[0054] Under salt treatment conditions, compared with those sprayed with distilled water, the plants sprayed with sericin solution grew better and were visibly taller. Figure 1 According to the measured data analysis, the relative growth rate of plant height of plants sprayed with sericin solution was significantly higher than that of plants sprayed with distilled water. Figure 2 As shown in A; the relative growth rate of stem diameter is significantly higher than that of the group sprayed with distilled water. Figure 2 As shown in C; the salt tolerance index of plant height and stem diameter were significantly higher than that of the group sprayed with distilled water. Figure 2 Medium B and Figure 2 As shown in D.

[0055] The root fresh weight and dry weight of the plants sprayed with sericin solution were significantly higher than those of the group sprayed with distilled water. Figure 2 ZhongE and Figure 2 As shown in G. Figure 2 As shown in Figure F, the relative reduction rate of biomass calculated by root fresh weight was significantly lower than that of the group sprayed with distilled water, indicating that spraying sericin solution weakened the effect of salt stress on plant root fresh weight biomass. Figure 2 As shown in H, the relative reduction rate of biomass calculated by root dry weight was significantly lower than that of the group sprayed with distilled water, and showed a negative average value, indicating that spraying sericin solution promoted the growth of root dry weight biomass under salt stress.

[0056] The aboveground fresh weight and dry weight of the plants sprayed with sericin solution were significantly higher than those sprayed with distilled water. Figure 2 Zhong I and Figure 2 As shown in K. Figure 2 J and Figure 2 As shown in Figure L, the relative reduction rate of biomass calculated by fresh weight and dry weight of the aboveground part was significantly lower than that of the group sprayed with distilled water, indicating that spraying sericin solution alleviated the effect of salt stress on the aboveground biomass of the plant.

[0057] The experimental results show that spraying sericin solution on the front of leaves can improve the salt tolerance of plants and enhance their growth under salt stress, which is manifested in the increase of plant height, stem thickness and biomass.

[0058] Experiment 2

[0059] The ability of spraying sericin solution on the back of leaves to improve the salt tolerance of plants was experimentally verified.

[0060] (I) Preparation of experimental materials

[0061] The experimental materials were prepared in the same way as in Experiment 1.

[0062] 2. Experimental design

[0063] The experimental design was the same as Example 7 except that the spraying treatment was spraying on the back of the leaves, that is, spraying distilled water on the back of the leaves, marked as HS2; and spraying sericin solution on the back of the leaves, marked as BS.

[0064] (III) Analysis of experimental results

[0065] This experimental case was treated with 350 mM NaCl solution for 18 days. Figure 3 The growth of plants sprayed with distilled water and sericin solution on the back of leaves. Figure 3 A in the middle is spraying distilled water on the back of leaves, HS2; Figure 3 Middle B is spraying sericin solution on the back of leaves, BS. Figure 4 For the measurement and analysis of various growth indicators.

[0066] like Figure 3 As shown in Figure 2, under salt treatment conditions, although there was little difference in the growth of plants sprayed with distilled water and sprayed with sericin solution, according to the measured data analysis, the relative growth rate of the plant height of the plants sprayed with sericin solution was significantly higher than that of the plants sprayed with distilled water. Figure 4 As shown in A, the relative growth rate of stem diameter was not significantly different from that of the group sprayed with distilled water. Figure 4 As shown in C. Combined with the analysis of plants grown under normal conditions, the salt tolerance index of plant height and stem diameter was significantly higher than that of the group sprayed with distilled water. Figure 4 As shown in B and 4D.

[0067] Compared with the group sprayed with distilled water, there was no significant difference in the root fresh weight and dry weight of the plants sprayed with sericin solution. Figure 4 ZhongE and Figure 4 As shown in G. There was no significant difference in the relative reduction rate of biomass calculated by root fresh weight and root dry weight. Figure 4 As shown in Figure 4F and Figure 4H, spraying sericin solution on the back of leaves had no significant effect on the root fresh weight biomass of plants under salt stress.

[0068] like Figure 4 As shown in J, I, K and L in the figure, although the relative reduction rate of fresh weight biomass of the aboveground part of the plants sprayed with sericin solution was significantly lower than that of the group sprayed with distilled water, the relative reduction rates of fresh weight, dry weight and root dry weight were not significantly different from those of the group sprayed with distilled water.

[0069] The experimental results show that spraying sericin solution on the back of leaves has limited effect on improving the salt tolerance of plants and cannot significantly promote the improvement of multiple growth indicators of plants under salt stress.

[0070] The results of Experiment 1 and Experiment 2 show that under salt stress conditions, spraying sericin solution on the front side of leaves can significantly improve the salt tolerance of plants. Sericin solution can be used as a spray component to improve the salt tolerance of crops planted in saline-alkali land.

[0071] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0072] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. Application of sericin liquid in improving salt tolerance of plants, characterized in that: The concentration of the sericin solution is 0.1 g / L to 1 g / L.

2. The use according to claim 1, characterized in that The concentration of the sericin solution is 0.2 g / L.

3. The use according to claim 1, characterized in that The sericin solution was sprayed on the front side of the leaves, with a spraying amount of 1 mL to 2 mL per plant each time.

4. The use according to claim 1, characterized in that The improvement of plant salt tolerance is manifested in the increase of plant height, stem thickness and biomass under salt stress environment.

5. The use according to claim 4, characterized in that The salt stress environment refers to a salt stress environment with a sodium chloride concentration of 100 mM to 500 mM.

6. The use according to claim 1, characterized in that The plants include tomatoes.

7. The use according to claim 1, characterized in that The method for preparing the sericin liquid comprises taking silkworm cocoons, placing them in water and boiling them to dissolve the sericin, and removing the undissolved silk to obtain the sericin liquid; The ratio of the clean silkworm cocoons to water is 5 g to 10 g: 200 mL.

8. A sericin plant foliar spray, characterized in that: The sericin solution according to claim 1.

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