Traditional Chinese medicine composition for killing flies and fly maggots as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention relates to the technical field of pest control, and provides a traditional Chinese medicine composition for killing flies and fly maggots, which is prepared from the following raw materials in parts by weight: 30 to 50 parts of fructus gleditsiae, 30 to 50 parts of fermented radix aconiti agrestis, 10 to 30 parts of white sugar and 10 to 20 parts of fructus broussonetiae. The invention further provides a preparation method of the traditional Chinese medicine composition and application of the traditional Chinese medicine composition in killing houseflies and fly maggots. The composition has the advantages that the fermented radix aconiti agrestis, the saponin, the white sugar and the fructus broussonetiae are adopted as the composition, compared with common radix aconiti agrestis, the fermented radix aconiti agrestis is smaller in toxicity, higher in safety and not prone to generating large harm to the health of livestock and poultry, but still keeps high pharmacological activity, and effective killing of houseflies and fly maggots is achieved; in addition, chemical reagents are not introduced, so that the flies cannot generate drug resistance, and the main components are degradable and environment-friendly.

Description

Technical Field

[0001] The invention relates to the technical field of pest control, and in particular to a Chinese medicinal composition for killing flies and fly maggots, and a preparation method and application thereof. Background Art

[0002] Houseflies are widely distributed in people's daily lives. Due to their strong reproductive ability, large populations of houseflies have appeared in farms and garbage dumps. Because houseflies have hairy bodies and their claw pads can secrete sticky substances, many pathogenic microorganisms can easily attach to the feet and body surface of houseflies. In livestock and poultry farms, pathogenic Escherichia coli, Pasteurella multocida, Leptospira and goose hepatitis virus can all be mechanically transmitted by houseflies. Because houseflies like to crawl and feed on corpses and feces, many pathogenic microorganisms and parasite cysts gather in the body of houseflies, and eventually the disease is biologically transmitted through the bites of houseflies. Among them, a typical example is that after livestock are infected with Sarcocystis, the cysts of Sarcocystis are eventually excreted from the body of livestock with feces. Flies crawl on the feces to find food and swallow the cysts into their bodies. The cysts mature in the body of flies and then enter the new host through the wounds on the body surface of other animals bitten by houseflies. Therefore, effectively controlling the reproduction of houseflies, especially killing them from the larval stage, is of great significance in reducing the spread of diseases.

[0003] However, current fly control methods mainly rely on chemical pesticides, which change the resistance of flies. In addition, chemical pesticides contain high levels of toxic ingredients and are difficult to degrade, which can easily cause great harm to the environment and the health of livestock and poultry.

[0004] Accordingly, it is urgent to seek a new fly killing method. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a Chinese medicine composition for killing flies and maggots, and its preparation method and application. The Chinese medicine composition can not only effectively kill houseflies and maggots, but also will not cause flies to develop drug resistance, and is environmentally friendly and not likely to cause significant harm to the health of livestock and poultry.

[0006] The present invention adopts the following technical solutions to solve the above technical problems:

[0007] A Chinese medicinal composition for killing flies and maggots comprises the following raw materials in parts by weight: 30-50 parts of sapodilla, 30-50 parts of fermented aconite, 10-30 parts of white sugar and 10-20 parts of paper mulberry seeds.

[0008] As one of the preferred embodiments of the present invention, the raw materials are included in the following weight proportions: 35 parts of saponins, 35 parts of fermented aconites, 15 parts of white sugar, and 12 parts of paper mulberry seeds.

[0009] As one of the preferred embodiments of the present invention, the raw materials are included in the following weight proportions: 40 parts of sapodilla, 40 parts of fermented aconite, 20 parts of white sugar, and 15 parts of paper mulberry seeds.

[0010] As one of the preferred embodiments of the present invention, the raw materials are included in the following weight proportions: 45 parts of saponins, 45 parts of fermented aconites, 25 parts of white sugar, and 18 parts of paper mulberry seeds.

[0011] As one of the preferred embodiments of the present invention, the fermented Aconitum kusnezoffii is solid fermented Aconitum kusnezoffii, and the preparation process is as follows: Aconitum kusnezoffii is collected and washed with water, and sterilized at 110-120°C for 25-35 minutes; when the temperature drops below 40°C, it is taken out and placed in a clean bench, and (0.5-1.5)×10 5 pfu / kg of Rhizopus japonicus strain (purchased from Shanghai Huzheng Biotechnology Co., Ltd.) was fermented at 30-40°C; after 4-6 days, it was dried at a constant temperature of 30-40°C, crushed and sieved.

[0012] As one of the preferred embodiments of the present invention, in the preparation of the solid fermented Aconitum kusnezoffii, high temperature sterilization is performed in an autoclave; fermentation and culture is performed in a constant temperature incubator; and pulverization is performed in a micro-plant pulverizer and passed through a 35-45 mesh sieve.

[0013] As one of the preferred embodiments of the present invention, the preparation process of the soap nuts is as follows: after the soap nuts are collected, they are washed with water, dried in the air, and placed in a drying oven to dry at 42-48° C.; then, they are crushed and sieved.

[0014] As one of the preferred embodiments of the present invention, in the preparation of the soap nuts, the soap nuts are placed in an electric constant temperature drying oven for drying; they are crushed by a micro plant crusher and passed through a 35-45 mesh sieve.

[0015] A method for preparing the above-mentioned traditional Chinese medicine composition comprises the following steps: adding 3 to 5 times the volume of water to the crushed sapodilla, fermented aconite and mulberry seed, stirring the mixture, and standing the mixture for 1 to 3 days to obtain a premix; and adding white sugar to the obtained premix and mixing the mixture evenly.

[0016] An application of the above traditional Chinese medicine composition in killing houseflies and fly maggots.

[0017] As one of the preferred methods of the present invention, when killing houseflies, the Chinese medicine composition is directly placed in a container and fixed at a fixed point; when killing fly maggots, the Chinese medicine composition is sprinkled in areas such as manure pits, garbage dumps, livestock and poultry farms where fly maggots breed, and 20 to 50 grams per square meter is sprinkled according to the density of fly maggots, which can effectively inhibit adult flies from crawling and laying eggs on them, and 98% of fly maggots can be eliminated in 2 to 3 days. When the temperature is between 25 and 35 degrees Celsius, houseflies reproduce rapidly, and the dosage should be appropriately increased.

[0018] As one of the preferred embodiments of the present invention, the best application condition is treatment with a 500 mg / mL concentration of the drug solution for 36 hours.

[0019] Efficacy of the raw materials of the present invention:

[0020] Gleditsia sinensis Lam: Gleditsia sinensis Lam is the dried mature fruit of the leguminous plant Gleditsia sinensis Lam, which is mainly produced in Henan, Jiangsu, Hubei and other places. It tastes spicy and salty, is warm in nature, enters the lung and large intestine meridians, is slightly toxic, and has the effects of expectoration, opening the mind, and reducing swelling and dispersing nodules. Modern pharmacological studies have shown that the ethanol extract of Gleditsia sinensis Lam has an inhibitory effect on pine caterpillars, but has no toxic activity, but has a good toxic effect on red spiders and cotton aphids, and has a good inhibitory effect on the germination of summer spores of wheat stem rust and leaf rust.

[0021] Fermented Aconite: Fermented Aconite is fermented by Rhizopus japonicus. Aconite is the dried root of Aconite kusnezofi Reichb, a perennial herbaceous plant of the Ranunculaceae family. Its main active ingredients are alkaloids (both biologically active and toxic substances, such as aconitine, mesaconitine, and mesaconitine). Currently, researchers have conducted systematic research on the extraction and insecticidal activity of Aconite alkaloids, and explored the stomach toxicity, antifeedant, contact killing, fumigation killing effects of aconite alkaloids on beet armyworms, as well as the growth inhibition effects on larvae. Through the fermentation of Rhizopus japonicus, the acetyl group at the C-8 position of the more toxic diester alkaloids (aconitine, meaconitine, and metsaconitine) in Aconitum kusnezoffii is hydrolyzed or decomposed to form less toxic monoester alkaloids (benzoylaconitine, benzoylmeaconitine, and benzoylhypaconitine), the toxicity of which is a few hundredths to a few thousandths of that of the diester alkaloids, or the benzoyl group at the C-14 position is further hydrolyzed to form correspondingly less toxic aconitines (aconitine, metsaconitine, and meaconitine), thereby reducing the toxicity of Aconitum kusnezoffii but still retaining its pharmacological activity.

[0022] White Sugar: White sugar tastes sweet and is used as an insect attractant.

[0023] Paper mulberry seeds: Paper mulberry seeds are also known as paper mulberry fruits. They are the mature fruits of the paper mulberry tree of the Moraceae family. They are mainly produced in Henan, Hubei, Shanxi, Gansu and other places. They are sweet, cold and non-toxic. They have the effects of clearing the liver and relieving heat, and promoting the flow of water and dampness. They are often used to treat liver and kidney yin deficiency, dizziness, edema, and urinary problems. Modern pharmacological studies have shown that paper mulberry seeds mainly include total saponins, flavonoids, alkaloids, fatty oils, red pigments, polysaccharides, trace elements, amino acids and other chemical components. In addition, in September and October every year when paper mulberry seeds mature, the fruit has an attractant effect on insects such as houseflies, and the special smell it emits attracts them to eat and lay eggs, which may be related to 2-n-pentylfuran, trans-2-hexenal and n-heptanal in volatile gases.

[0024] The advantages of the present invention compared to the prior art are:

[0025] (1) The present invention uses fermented Aconitum kusnezoffii, Gleditsia sinensis, white sugar, and Cuscuta chinensis as a composition, wherein fermented Aconitum kusnezoffii and Gleditsia sinensis play a major role in killing houseflies and fly maggots, and white sugar and Cuscuta chinensis serve as attractants; at the same time, the fermented Aconitum kusnezoffii of the present invention is less toxic and safer than ordinary Aconitum kusnezoffii, and is not likely to cause significant harm to the health of livestock and poultry, but still retains a high pharmacological activity, achieving effective killing of houseflies and fly maggots; the two attractants of white sugar and Cuscuta chinensis of the present invention are better than honey in attracting flies;

[0026] (2) The present invention does not introduce any chemical reagents, will not cause flies to develop drug resistance, and the main components are degradable and environmentally friendly;

[0027] (3) The present invention provides a wider range of usage scenarios, lower toxicity, and more efficient killing, which is more advantageous than previous products. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is the number of eggs successfully hatched after 24 hours of treatment with different groups of liquid medicine in Experiment 3 (in the figure, the same letters indicate no significant difference between the groups, P>0.05; different letters indicate significant difference between the groups, P<0.05);

[0029] Figure 2 This is a comparison chart of the number of houseflies attracted by different groups of Chinese medicines in Experimental Example 5 within 8 hours (in the figure, the same letters indicate no significant difference between the groups, P>0.05; different letters indicate significant difference between the groups, P<0.05). DETAILED DESCRIPTION

[0030] The following is a detailed description of the embodiments of the present invention. This embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation method and a specific operation process are given, but the protection scope of the present invention is not limited to the following embodiments. At the same time, the raw materials, bacterial agents, equipment and methods used in the following embodiments, unless otherwise specified, are all conventional commercially available raw materials, bacterial agents, equipment and methods in the field, and will not be repeated.

[0031] Example 1

[0032] A traditional Chinese medicine composition for killing flies and maggots in this embodiment comprises the following raw materials in parts by weight: 30 parts of sapodilla, 30 parts of fermented aconite, 10 parts of white sugar, and 10 parts of paper mulberry seeds.

[0033] Preparation method:

[0034] (1) Solid fermentation of Aconitum kusnezoffii:

[0035] After being collected, Aconitum kusnezoffii was washed with water and sterilized in an autoclave at 110°C for 35 min. When the temperature dropped below 40°C, it was taken out and placed in a clean bench. 0.5×10 5 pfu / kg of Rhizopus japonicus strains were fermented and cultured at 30°C for 6 days; dried at a constant temperature of 30°C, crushed with a micro-plant crusher, and passed through a 35-mesh sieve; put into a plastic film bag, sealed, and stored in a 4°C refrigerator for use.

[0036] (2) Gleditsia treatment:

[0037] After the soap beans are collected, they are washed with water, dried, and placed in an electric constant temperature drying oven at 42°C for drying; then, they are crushed using a micro-plant grinder and passed through a 35-mesh sieve; they are placed in a plastic film bag, sealed, and placed in a 4°C refrigerator for storage until use.

[0038] (3) Paper mulberry seed processing:

[0039] Collect the mature paper mulberry seeds, rinse the fruits three times with distilled water, grind them in a plant grinder to obtain paper mulberry seed pulp, and store them in a 4°C refrigerator for later use.

[0040] (4) Add 3 times the volume of water to the fermented Aconitum kusnezoffii, Gleditsia sinensis and Cucurbitacinthus chinensis pulp obtained in the above treatment steps, stir and let stand for 1 day to obtain a premix; add sugar to the obtained premix and mix well.

[0041] Example 2

[0042] A traditional Chinese medicine composition for killing flies and maggots in this embodiment comprises the following raw materials in parts by weight: 40 parts of sapodilla, 40 parts of fermented aconite, 20 parts of white sugar, and 15 parts of paper mulberry seeds.

[0043] Preparation method:

[0044] (1) Solid fermentation of Aconitum kusnezoffii:

[0045] After being collected, Aconitum kusnezoffii was washed with water and sterilized in an autoclave at 115°C for 30 min. When the temperature dropped below 40°C, it was taken out and placed in a clean bench. 1.0×10 5 pfu / kg of Rhizopus japonicus strains were fermented and cultured at 37°C for 5 days; dried at a constant temperature of 37°C, crushed with a micro-plant crusher, and passed through a 40-mesh sieve; packed into a plastic film bag, sealed, and stored in a 4°C refrigerator for use.

[0046] (2) Gleditsia treatment:

[0047] After being collected, the soap beans are washed with water, dried, and placed in an electric constant temperature drying oven at 45°C for drying; then, they are crushed using a micro-plant grinder and passed through a 40-mesh sieve; they are put into a plastic film bag, sealed, and placed in a 4°C refrigerator for storage until use.

[0048] (3) Paper mulberry seed processing:

[0049] Collect the mature paper mulberry seeds, rinse the fruits three times with distilled water, grind them in a plant grinder to obtain paper mulberry seed pulp, and store them in a 4°C refrigerator for later use.

[0050] (4) Add 4 times the volume of water to the fermented Aconitum kusnezoffii, Gleditsia sinensis and Cucurbitacinthus chinensis pulp obtained in the above treatment steps, stir and let stand for 2 days to obtain a premix; add sugar to the obtained premix and mix well.

[0051] Example 3

[0052] A traditional Chinese medicine composition for killing flies and maggots in this embodiment comprises the following raw materials in parts by weight: 35 parts of sapodilla, 35 parts of fermented aconite, 15 parts of white sugar, and 12 parts of paper mulberry seeds.

[0053] Preparation method: Same as Example 2.

[0054] Example 4

[0055] A Chinese medicinal composition for killing flies and maggots in this embodiment comprises the following raw materials in parts by weight: 45 parts of sapodilla, 45 parts of fermented aconite, 25 parts of white sugar, and 18 parts of paper mulberry seeds.

[0056] Preparation method: Same as Example 2.

[0057] Example 5

[0058] A traditional Chinese medicine composition for killing flies and maggots in this embodiment comprises the following raw materials in parts by weight: 50 parts of sapodilla, 50 parts of fermented aconite, 30 parts of white sugar, and 20 parts of paper mulberry seeds.

[0059] Preparation method:

[0060] (1) Solid fermentation of Aconitum kusnezoffii:

[0061] After being collected, Aconitum kusnezoffii was washed with water and sterilized in an autoclave at 120°C for 25 min. When the temperature dropped below 40°C, it was taken out and placed in a clean bench. 1.5×10 5 pfu / kg of Rhizopus japonicus strains were fermented and cultured at 40°C for 4 days; dried at a constant temperature of 40°C, crushed with a micro-plant crusher, and passed through a 45-mesh sieve; packed into a plastic film bag, sealed, and stored in a 4°C refrigerator for use.

[0062] (2) Gleditsia treatment:

[0063] After the soap beans are collected, they are washed with water, dried, and placed in an electric constant temperature drying oven at 48°C for drying; then, they are crushed using a micro-plant grinder and passed through a 45-mesh sieve; they are placed in a plastic film bag, sealed, and placed in a 4°C refrigerator for storage until use.

[0064] (3) Paper mulberry seed processing:

[0065] Collect the mature paper mulberry seeds, rinse the fruits three times with distilled water, grind them in a plant grinder to obtain paper mulberry seed pulp, and store them in a 4°C refrigerator for later use.

[0066] (4) Add 5 times the volume of water to the fermented Aconitum kusnezoffii, Gleditsia sinensis and Cucurbitacinthus chinensis pulp obtained in the above treatment steps, stir and let stand for 3 days to obtain a premix; add sugar to the obtained premix and mix well.

[0067] Example 6

[0068] The present embodiment provides an application of a Chinese medicinal composition for killing flies and fly maggots.

[0069] When killing houseflies, the Chinese medicinal composition of the above embodiment is directly placed in a container and placed at a fixed point.

[0070] When killing fly maggots, the Chinese medicine composition is sprayed in areas such as manure pits, garbage dumps, livestock and poultry farms where fly maggots breed, and 20 to 50 grams per square meter is sprayed according to the density of fly maggots.

[0071] Comparative Example 1

[0072] A Chinese medicine composition in this comparative example is basically the same as Example 2, the main difference being that no "saponin" is added.

[0073] Comparative Example 2

[0074] A Chinese medicine composition in this comparative example is basically the same as Example 2, the main difference being that: no "fermented aconite" is added.

[0075] Comparative Example 3

[0076] A Chinese medicine composition in this comparative example is basically the same as Example 2, with the main difference being that "ordinary Aconitum kusnezoffii" that has not been subjected to solid fermentation treatment is used instead of "fermented Aconitum kusnezoffii".

[0077] Comparative Example 4

[0078] A Chinese medicine composition in this comparative example is basically the same as Example 2, the main difference being that "honey" is used instead of "white sugar + paper mulberry seeds".

[0079] Comparative Example 5

[0080] A Chinese medicine composition in this comparative example is basically the same as Example 2, the main difference being that no "Curcuma mulberry seed" is added.

[0081] Comparative Example 6

[0082] A Chinese medicine composition in this comparative example is basically the same as Example 2, the main difference being that no "white sugar" is added.

[0083] Experimental Example 1

[0084] This experimental example is used to verify the indoor adult fly killing effect of Chinese medicinal agents (the Chinese medicinal composition of the present invention) of different concentrations.

[0085] 1. Experimental Methods

[0086] (1) Collect houseflies. Place fresh pig liver in the bottom box under the fly trap and place it outdoors to lure houseflies to forage. On the same day, transfer the captured adult flies to a 1000 mL triangular flask and feed them with "cotton balls soaked in sugar water" and allow them to lay eggs. When the larvae develop to 3 days old, use a fine brush to move them into a larval feed tank for feeding (the larval feed ingredients are as follows: 600 g wheat bran, 100 g fish meal, 40 g milk powder, 8 g yeast powder, 15 g peptone, 1500 mL water; after mixing, divide them into feed tanks, sterilize them and place them in a 4°C refrigerator for later use). When the larvae are about to pupate, pour the feed and larvae into a plastic basin, tie the basin with white gauze, and let them pupate. Collect the fly pupae, place them in an insect cage, and let them emerge. Feed the adult flies with white sugar and milk powder, and place a small amount of drinking water.

[0087] The rearing conditions are: temperature (27±1)°C, relative humidity (70±5)%, light cycle (L:D=14L:10D). After rearing for many generations, healthy adult flies with uniform morphology and size were selected as the test insect source.

[0088] (2) Determination by feed mixing method. The Chinese medicine composition of the present invention (using Example 2 as an example) was prepared into solutions with concentrations of 500, 250, 125, 62.5, 31.25, and 15.63 mg / mL with distilled water, and sucrose and distilled water were mixed into sugar water at a ratio of 1:10; 10 mL of the drug solution was taken and mixed with an equal amount of sugar water, and the mixture was used as feed to feed houseflies (the sugar water mixed with the drug solution was immersed in cotton to prevent the test insects from drowning); the sugar water was used as the control group, and 3 replicates were set for each concentration treatment, and the test insects were placed in a 2L wide-mouth bottle, with 100 adult fly test insects starved for 8 hours in each group, and the number of dead insects after 12, 24, 36, and 48 hours was observed and recorded, and the mortality rate and corrected mortality rate were calculated according to formulas (1-1) and (1-2), and toxicity regression analysis was performed for different treatment times.

[0089] Mortality rate = number of dead insects / total number of insects × 100% (1-1);

[0090] Adjusted mortality rate = (treatment mortality rate - control mortality rate) / (1 - control mortality rate) x 100% (1-2).

[0091] 2. Experimental Results

[0092] After being treated with different concentrations of Chinese herbal mixtures and sugar water, houseflies died to varying degrees. The adjusted mortality results are shown in Table 1.

[0093] Table 1 Corrected mortality of houseflies killed by Chinese herbal medicines of different concentrations at different times

[0094]

[0095] Note: The same letters after the data in the same column indicate no significant difference (P>0.05); different letters indicate significant difference (P<0.05).

[0096] As shown in Table 1, when the concentration was 500 mg / mL and the treatment time was 36 h, the corrected mortality rate was 100%, and each treatment group was significantly higher than the control group (P<0.05). The comprehensive toxicity is the toxic effect of the combination of multiple modes of action of the Chinese medicine mixture on houseflies. From the results, it can be seen that the corrected mortality rate of houseflies increases continuously with the increase of concentration. Therefore, appropriately increasing the concentration of the Chinese medicine mixture can enhance the comprehensive toxicity effect.

[0097] Next, the comprehensive toxic effect of the Chinese herbal mixture on houseflies was further explained. The housefly-corrected mortality results of the Chinese herbal mixture treated with poison at different times were subjected to regression analysis. The corrected mortality of the treatment results was taken as Y and the logarithm of the different experimental concentrations was taken as x to make a toxicity regression equation, and the LC50 was calculated. The results are shown in Table 2.

[0098] Table 2 Regression analysis of the toxicity of Chinese herbal medicine mixture to houseflies at different killing times

[0099]

[0100] In Table 2, the correlation coefficient of the regression equation (R 2) are all above 0.90, and the chi-square test P values ​​are all greater than 0.15, indicating that the fitting degree of the regression equation is good, indicating that the equation has a high credibility, and the LC50 values ​​calculated from this are relatively accurate. Combining Tables 1 and 2, it can be seen that the LC50 of houseflies treated by mixed feeding method for 12, 24, 36, and 48 hours are 516.774, 263.049, 204.463, and 156.465 mg / mL, respectively. The LC50 of mixed feeding method for 12 hours is 3.3 times that of 48 hours. As time goes by, the LC50 continues to decrease, indicating that Chinese medicine needs a certain amount of time to have the best effect, and appropriately increasing the treatment time can increase the poisoning effect. But in short, the best application condition of the Chinese medicine composition of the present invention is 500 mg / mL concentration liquid treatment for 36 hours.

[0101] Experimental Example 2

[0102] This experimental example is used to verify the effect of different Chinese medicine composition formulas on the killing effect of adult flies.

[0103] 1. Experimental Methods

[0104] Referring to the method of Experimental Example 1, the adult flies in the experiment of Experimental Example 1 were collected and evenly divided into 5 groups. Each group of flies was fed according to the following method:

[0105] Group 1 (blank control group): sugar water;

[0106] Group 2: 10 mL of the drug solution of the present invention (Example 2, 500 mg / mL) + 10 mL of sugar water;

[0107] Group 3: 10 mL of comparative example 1 solution (500 mg / mL) + 10 mL of sugar water;

[0108] Group 4: 10 mL of comparative example 2 solution (500 mg / mL) + 10 mL of sugar water;

[0109] Group 5: 10 mL of the drug solution of Comparative Example 3 (500 mg / mL) + 10 mL of sugar water.

[0110] Observe and record the number of dead insects after 12, 24, 36, and 48 hours.

[0111] 2. Experimental Results

[0112] As shown in Table 3.

[0113] Table 3 Corrected mortality of houseflies killed by different formulations at different times

[0114]

[0115] Note: The same letters after the data in the same column indicate no significant difference (P>0.05); different letters indicate significant difference (P<0.05).

[0116] It can be seen from Table 3 that: (1) In the formula of the Chinese medicine composition of the present invention, fermented Aconitum kusnezoffii and Gleditsia sinensis play the main killing role, and the combined effect is the best; (2) The fermented Aconitum kusnezoffii of the present invention still retains a higher pharmacological activity than ordinary Aconitum kusnezoffii, and effectively kills adult flies.

[0117] Experimental Example 3

[0118] This experimental example is used to verify the effect of different Chinese medicine composition formulas on the inhibition of insect egg hatching.

[0119] 1. Experimental Methods

[0120] The eggs of the housefly in Experimental Example 1 were collected and evenly divided into 5 groups, and each group was treated as follows:

[0121] Group 1 (blank control group): 0.5 mL distilled water + 5 g larval feed;

[0122] Group 2: 0.5 mL of the drug solution of the present invention (Example 2, 500 mg / mL) + 5 g of larval feed;

[0123] Group 3: 0.5 mL of the drug solution of Comparative Example 1 (500 mg / mL) + 5 g of larval feed;

[0124] Group 4: 0.5 mL of the drug solution of Comparative Example 2 (500 mg / mL) + 5 g of larval feed;

[0125] Group 5: 0.5 mL of the drug solution of Comparative Example 3 (500 mg / mL) + 5 g of larval feed.

[0126] Take 0.05g of unhatched eggs and place them in the center of each group of feed, seal the box lid, and record the number of hatched eggs in each group after 24 hours. Repeat 3 times.

[0127] 2. Experimental results

[0128] like Figure 1 shown.

[0129] Depend on Figure 1 It can be seen that: (1) in the formula of the Chinese medicine composition of the present invention, fermented Aconitum kusnezoffii and Gleditsia sinensis play the main inhibitory role, and the combined effect is the best; (2) compared with ordinary Aconitum kusnezoffii, the fermented Aconitum kusnezoffii of the present invention still retains a higher pharmacological activity, achieving effective inhibition of insect egg hatching.

[0130] Experimental Example 4

[0131] This experimental example is used to verify the effect of different Chinese medicine composition formulas on the killing effect of indoor fly maggots.

[0132] 1. Experimental Methods

[0133] The housefly larvae in Experimental Example 1 were taken, washed and then active 3-day-old fly maggots were selected and divided into 5 groups. The feed formula for each group was as follows:

[0134] Group 1 (blank control group): 0.5 mL distilled water + 5 g larval feed;

[0135] Group 2: 0.5 mL of the drug solution of the present invention (Example 2, 500 mg / mL) + 5 g of larval feed;

[0136] Group 3: 0.5 mL of the drug solution of Comparative Example 1 (500 mg / mL) + 5 g of larval feed;

[0137] Group 4: 0.5 mL of the drug solution of Comparative Example 2 (500 mg / mL) + 5 g of larval feed;

[0138] Group 5: 0.5 mL of the drug solution of Comparative Example 3 (500 mg / mL) + 5 g of larval feed.

[0139] Each group of feed was placed in a 50 mL beaker, and 20 test maggots were placed in each beaker. A gauze mesh cover (with the same inner diameter as the beaker) was placed above the liquid level in the beaker. The beaker was observed continuously for 72 hours. The absence of peristalsis or contraction after needle prick was considered death, and the number of dead maggots was recorded.

[0140] 2. Experimental Results

[0141] The results are shown in Table 4.

[0142] Table 4 The number of maggot deaths in different groups of formulations

[0143]

[0144] Note: The same letters after the data in the same column indicate no significant difference (P>0.05); different letters indicate significant difference (P<0.05).

[0145] It can be seen from Table 4 that: (1) In the formula of the Chinese medicine composition of the present invention, fermented Aconitum kusnezoffii and Gleditsia sinensis play the main killing role, and the combined effect is the best; (2) The fermented Aconitum kusnezoffii of the present invention still retains a higher pharmacological activity than ordinary Aconitum kusnezoffii, and effectively kills fly maggots.

[0146] Experimental Example 5

[0147] This experimental example is used to verify the effects of different Chinese medicine composition formulas on fly attracting effects.

[0148] 1. Experimental Methods

[0149] The Chinese medicine compositions of the present invention and comparative examples 4, 5 and 6 were divided into groups 1, 2, 3 and 4, respectively, and the fly attracting effect of each group of formulas was tested.

[0150] The specific method is:

[0151] In a relatively closed space (volume about 1m 3 The experiment was conducted in a glass box with good ventilation and relatively stable temperature and humidity (temperature 25±2℃, relative humidity 65±5%). Five identical small containers (such as small plastic bowls) were placed in the four corners and the center of the glass box.

[0152] For each group, an appropriate amount (about 5 g) of the corresponding traditional Chinese medicine composition was placed in five small containers respectively.

[0153] A light source (40 W incandescent bulb) was placed outside the glass box, about 0.5 m away from the glass box, to simulate the light in the natural environment to attract flies.

[0154] The experimental time was set from 9 am to 5 pm, and the observation was continuous for 8 hours, and the number of flies attracted around each container was recorded. Repeated 3 times.

[0155] 2. Experimental Results

[0156] The results are as follows Figure 2 shown.

[0157] Depend on Figure 2 It can be seen that: (1) in the formula of the Chinese medicine composition of the present invention, white sugar and paper mulberry seeds are used as attractants, and the combined effect is the best; (2) the two attractants of white sugar and paper mulberry seeds of the present invention are better than honey in attracting flies.

[0158] Experimental Example 6

[0159] This experimental example is used to verify the low toxicity of the Chinese medicine composition of the present invention to poultry and livestock.

[0160] 1. Experimental Methods

[0161] The low toxicity of the composition of the present invention and comparative example 3 to poultry and livestock was tested respectively. The specific method is:

[0162] Experimental animal selection and grouping: 60 healthy 5-day-old chickens were selected and randomly divided into three groups, with 20 chickens in each group. The feeding formula for each group is as follows:

[0163] Group 1 (blank control group): intragastrically administered with 0.3 g / kg body weight of normal saline + free access to basic diet and drinking water;

[0164] Group 2: intragastrically administered with 0.3 g / kg body weight of the Chinese medicine composition of Example 2 of the present invention + free access to basic diet and drinking water;

[0165] Group 3: intragastric administration of 0.3 g / kg body weight of the Chinese medicine composition of Comparative Example 3 of the present invention + free access to basic diet and drinking water;

[0166] Observation indexes and time: The weight of the chickens was measured at the beginning of the experiment, and the weight changes of the chickens were recorded on the 4th, 7th, 10th and 14th days of administration. On the 7th and 14th days after administration, 10 chickens were randomly selected from each group, and blood was collected to detect biochemical indicators such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and urea nitrogen (BUN) in the blood to evaluate liver and kidney function.

[0167] 2. Experimental Results

[0168] The results are shown in Tables 5 and 6.

[0169] Table 5 Effects of different group formulas on chicken body weight

[0170] Group Initial experiment (g) Section 4d(g) Article 7d(g) Article 10d(g) Article 14d(g) Group 1 120.5±5.2a 148.2±3.5a 198.5±4.2a 295.6±5.8a 495.3±6.5a Group 2 119.8±4.5a 143.8±3.0a 193.2±3.8a 288.5±5.2a 482.1±6.0a Group 3 120.2±5.0a 108.5±2.8b 145.6±3.2c 198.2±4.5d 345.8±5.5e

[0171] Note: The same letters after the data in the same column indicate no significant difference (P>0.05); different letters indicate significant difference (P<0.05).

[0172] Table 6 Effects of different group formulas on biochemical indicators of chickens

[0173]

[0174] Note: The same letters after the data in the same column indicate no significant difference (P>0.05); different letters indicate significant difference (P<0.05).

[0175] It can be seen from Table 5 and Table 6 that the fermented Aconitum kusnezoffii of the present invention is less toxic than the common Aconitum kusnezoffii, has little effect on growth and development, is safer, and is not likely to cause significant harm to the health of livestock and poultry.

[0176] In summary, the Chinese medicine composition of the present invention provides a wider range of usage scenarios, lower toxicity, and more efficient killing, and has more advantages than previous products.

[0177] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A Chinese medicinal composition for killing flies and maggots, characterized in that: The invention comprises the following raw materials in parts by weight: 30-50 parts of sapodilla, 30-50 parts of fermented aconite, 10-30 parts of white sugar and 10-20 parts of paper mulberry seeds.

2. The Chinese medicinal composition for killing flies and maggots according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 35 parts of sapodilla, 35 parts of fermented aconite, 15 parts of white sugar and 12 parts of paper mulberry seeds.

3. The Chinese medicinal composition for killing flies and maggots according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 40 parts of sapodilla, 40 parts of fermented aconite, 20 parts of white sugar and 15 parts of paper mulberry seeds.

4. The Chinese medicinal composition for killing flies and maggots according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 45 parts of sapodilla, 45 parts of fermented aconite, 25 parts of white sugar and 18 parts of paper mulberry seeds.

5. The Chinese medicinal composition for killing flies and maggots according to any one of claims 1 to 4, characterized in that: The fermented aconite is solid fermented aconite, and the preparation process is as follows: after the aconite is collected, it is washed with water and sterilized at 110-120° C. for 25-35 minutes; when the temperature drops below 40° C., it is taken out and placed in a clean bench, and (0.5-1.5)×10 5 pfu / kg of Rhizopus japonicus strains are fermented and cultured at 30-40°C; after 4-6 days, the mixture is dried at a constant temperature of 30-40°C, crushed, and sieved.

6. The Chinese medicinal composition for killing flies and maggots according to claim 5, characterized in that: In the preparation of the solid fermented Aconitum kusnezoffii, a high-temperature sterilization is performed in an autoclave; fermentation and culture are performed in a constant temperature incubator; and the powder is crushed in a micro-plant crusher and passed through a 35-45 mesh sieve.

7. The Chinese medicinal composition for killing flies and maggots according to any one of claims 1 to 4, characterized in that: The preparation process of the soap nuts is as follows: after the soap nuts are collected, they are washed with water, dried in the air, and placed in a drying oven to dry at 42-48° C.; then, they are crushed and sieved.

8. The Chinese medicinal composition for killing flies and maggots according to claim 7, characterized in that: In the preparation of the soapberry, the soapberry is placed in an electric constant temperature drying oven for drying; it is crushed by a micro-plant crusher and passed through a 35-45 mesh sieve.

9. A method for preparing the Chinese medicine composition according to any one of claims 1 to 8, characterized in that: Add 3 to 5 times the volume of water to the crushed sapodilla, fermented aconite and paper mulberry seeds, stir and let stand for 1 to 3 days to obtain a premix; add white sugar to the obtained premix and mix well.

10. Use of the traditional Chinese medicine composition according to any one of claims 1 to 8 in killing houseflies and fly maggots.

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