Corn stalk fermented feed and preparation method thereof

Abstract

The invention discloses a corn stalk fermented feed and a preparation method thereof, and belongs to the field of fermented feed preparation. The corn stalks are used as a main material of the base material and are supplemented with the vinegar residues and the bran for co-fermentation, the fermented feed which is rich in fragrance and soft in texture is obtained, the palatability is good, the nutrition balance degree of the fermented feed can be improved, livestock breeding of cattle and sheep can be better met, and the economic benefits are increased. Through the synergistic effect of the added compound enzyme agent and the compound microbial agent, the utilization rate of the base material is increased, the content of fermentation protein is increased, the palatability is obviously improved, the feed intake and the digestibility are increased, more organic acids can be generated in the primary fermentation process and the secondary fermentation process, the corrosion resistance of the fermented feed is improved, the immunity of animal bodies is enhanced, and meanwhile, the effect of improving the quality of the fermented feed is achieved. Microorganisms in the complex microbial inoculant also contain a large amount of amino acids, vitamins and minerals, so that the nutritional value of the fermented feed can be increased, the intestinal flora balance of ruminants can be regulated, and the growth of pathogenic bacteria can be inhibited.

Description

Technical Field

[0001] The invention relates to the technical field of feed preparation, and in particular to a corn stalk fermented feed and a preparation method thereof. Background Art

[0002] Corn stalks contain a large amount of fiber components (50% to 70%) and a small amount of crude protein (5% to 10%) and crude fat. Cellulose is polymerized from glucose monomers, and the monomers of hemicellulose polymers are mainly xylose. The digestible energy in corn stalks is about 534kcal / kg, which is similar to the energy contained in forage grass. Lignin is an important component of lignocellulosic raw materials besides cellulose and hemicellulose. Its function is to support the plant cell wall and bury cellulose and hemicellulose. Due to its low permeability, it forms a natural barrier for plant cells, protects the fiber components from the action of enzymes and microbial invasion, and reduces the rumen digestibility of feed. Corn stalks have a low crude protein content and lack other necessary nutrients such as vitamins. Due to the unbalanced nutritional components, poor palatability, low feeding rate and low digestibility of corn stalks, the direct feeding effect is poor, which is only equivalent to 25% to 50% of forage grass. In order to improve its nutritional value, corn stalks need to be processed. Traditional processing technologies include physical methods, chemical methods, and microbial treatment methods. Among them, the physical method is relatively simple through cutting, grinding, and cooking. It can appropriately improve the palatability of corn stalks and increase the amount of feed intake, but it cannot improve the nutritional value and digestibility of corn stalks. In addition, there is also a puffing method, but the lignin in the corn stalks can only be softened or partially hydrolyzed when the temperature reaches 170°C, which requires specific equipment to transport heat and is costly. Chemical methods include acid-base treatment methods, ammoniation methods, oxidant treatment methods, etc., but there are generally problems such as limited use of reagents, safety needs to be improved, and high costs that are difficult to promote. Biological treatment degrades lignin by adding microorganisms, but there is a problem of unstable reaction system, which makes the fermented products unable to meet nutritional needs. Summary of the invention

[0003] Based on this, in order to solve the technical problem that the fermentation effect of corn stalk fermented feed in the prior art is not ideal and the nutrition cannot meet the breeding needs, the present invention provides a corn stalk fermented feed and a preparation method thereof, and the specific technical scheme is as follows:

[0004] A corn stalk fermented feed, wherein the corn stalk fermented feed is obtained by treating a base material with a composite enzyme agent and a composite bacterial agent;

[0005] The base material comprises the following raw materials in parts by weight: 50 to 80 parts of corn stalks, 20 to 30 parts of vinegar lees, and 15 to 20 parts of bran;

[0006] The composite enzyme preparation is composed of xylanase, α-glucosidase and a liquid carrier, wherein the enzyme activity of the xylanase is 1000u / g to 1200u / g, and the enzyme activity of the α-glucosidase is 800u / g to 1200u / g;

[0007] The composite bacterial agent is obtained by activation treatment of raw materials including 7 to 10 parts of Bacillus subtilis, 3 to 5 parts of brewer's yeast, 5 to 10 parts of Aspergillus niger, 5 to 7 parts of sucrose and 60 to 85 parts of water.

[0008] Furthermore, the liquid carrier comprises the following ingredients: 0.1 g / mL to 0.2 g / mL of glycerol, 0.1 g / mL to 0.5 g / mL of glucose, and 0.01 g / mL to 0.05 g / mL of dextrin.

[0009] Furthermore, the activation treatment is as follows: Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus niger, sucrose and water are mixed, stirred at 35°C to 38°C for 1min to 2min, and then activated at 28°C to 35°C for 5h to 8h to obtain a composite bacterial agent.

[0010] Furthermore, the added amount of the complex enzyme agent accounts for 1% to 10% of the mass of the base material.

[0011] Furthermore, the added amount of the composite bacterial agent accounts for 1% to 10% of the mass of the base material.

[0012] In addition, the present invention also provides a method for preparing corn stalk fermented feed, the preparation method comprising the following steps:

[0013] The corn stalks are crushed, and then vinegar dregs and bran are added and mixed evenly to obtain a base material;

[0014] Add the composite enzyme to the base material, stir evenly, and perform enzymolysis for 20 h to 25 h at a temperature of 40° C. to 55° C. and a pH of 4.5 to 6.0, and then inactivate the enzyme to obtain a mixture A;

[0015] The mixture A is added into a fermentation kettle, and a composite bacterial agent is added for primary fermentation, wherein the temperature of the primary fermentation is 28° C. to 30° C., the time is 20 h to 48 h, and the mixture is stirred every 2 h, to obtain a mixture B;

[0016] The composite bacterial agent, calcium chloride and sodium chloride are continuously added to the mixture B for secondary fermentation, and the temperature of the secondary fermentation is 28° C. to 32° C., the time is 1 day to 5 days, and stirring is performed every 2 hours. After the fermentation is completed, corn stalk fermented feed is obtained.

[0017] Furthermore, in the primary fermentation, the added amount of the composite bacterial agent accounts for 1% to 5% of the mass of the base material.

[0018] Furthermore, in the secondary fermentation, the added amount of the composite bacterial agent accounts for 2% to 15% of the mass of the base material.

[0019] Furthermore, the added amount of the calcium chloride accounts for 0.1% to 0.5% of the mass of the base material, and the added amount of the sodium chloride accounts for 1% to 7% of the mass of the base material.

[0020] Furthermore, during the primary fermentation and the secondary fermentation, the humidity is controlled at 30% to 40%.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. The present invention uses corn stalks as the main material of the base material, and ferments it with vinegar dregs and bran to obtain a fermented feed with a strong aroma and soft texture. It not only has good palatability, but also can improve the nutritional balance of the fermented feed, and can better meet the animal husbandry of cattle and sheep.

[0023] 2. The composite enzyme of the present invention has stable performance and can exert a better enzymatic hydrolysis effect in the enzymatic hydrolysis system, destroy cell walls, and decompose hemicellulose and lignin, which has a positive effect on subsequent primary fermentation and secondary fermentation.

[0024] 3. The present invention helps to improve the utilization rate of the base material, increase the fermentation protein content, significantly improve the palatability, increase the feed intake and digestibility by adding a composite enzyme agent and a composite bacterial agent. In addition, during the primary fermentation and secondary fermentation process, more organic acids can be produced, the antiseptic ability of the fermented feed is improved, and the immunity of the animal body is enhanced. At the same time, the microorganisms in the composite bacterial agent themselves also contain a large amount of amino acids, vitamins, and minerals, which can not only increase the nutritional value of the fermented feed, but also regulate the balance of the intestinal flora of ruminants and inhibit the growth of pathogenic bacteria. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with its embodiments. It should be understood that the specific implementation methods described herein are only used to explain the present invention and do not limit the scope of protection of the present invention.

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0027] A corn stalk fermented feed in one embodiment of the present invention is obtained by treating a base material with a composite enzyme agent and a composite bacterial agent;

[0028] The base material comprises the following raw materials in parts by weight: 50 to 80 parts of corn stalks, 20 to 30 parts of vinegar lees, and 15 to 20 parts of bran;

[0029] The composite enzyme preparation is composed of xylanase, α-glucosidase and a liquid carrier, wherein the enzyme activity of the xylanase is 1000u / g to 1200u / g, and the enzyme activity of the α-glucosidase is 800u / g to 1200u / g;

[0030] The composite bacterial agent is obtained by activation treatment of raw materials including 7 to 10 parts of Bacillus subtilis, 3 to 5 parts of brewer's yeast, 5 to 10 parts of Aspergillus niger, 5 to 7 parts of sucrose and 60 to 85 parts of water.

[0031] In one embodiment, the weight ratio of the xylanase, α-glucosidase and liquid carrier is (1-15):(10-25):100.

[0032] In one embodiment, the liquid carrier comprises the following ingredients: 0.1 g / mL to 0.2 g / mL glycerol, 0.1 g / mL to 0.5 g / mL glucose, and 0.01 g / mL to 0.05 g / mL dextrin.

[0033] In one embodiment, the activation treatment is: mixing Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus niger, sucrose and water, stirring at 35°C to 38°C for 1min to 2min, and then activating at 28°C to 35°C for 5h to 8h to obtain a composite bacterial agent.

[0034] In one embodiment, the added amount of the complex enzyme agent accounts for 1% to 10% of the mass of the base material.

[0035] In one embodiment, the added amount of the composite bacterial agent accounts for 1% to 10% of the mass of the base material.

[0036] In one embodiment, the viable bacteria count of Bacillus subtilis in the composite bacterial agent is 1.2×10 9 CFU / kg~1.9×10 11 CFU / kg; the viable count of the brewer's yeast is 1.4×10 9 CFU / kg~1.3×10 11 CFU / kg; the viable count of Aspergillus niger was 1.4×10 9 CFU / kg~1.4×10 11 CFU / kg.

[0037] In addition, the present invention also provides a method for preparing corn stalk fermented feed, the preparation method comprising the following steps:

[0038] The corn stalks are crushed, and then vinegar dregs and bran are added and mixed evenly to obtain a base material;

[0039] Add the composite enzyme to the base material, stir evenly, and perform enzymolysis for 20 h to 25 h at a temperature of 40° C. to 55° C. and a pH of 4.5 to 6.0, and then inactivate the enzyme to obtain a mixture A;

[0040] The mixture A is added into a fermentation kettle, and a composite bacterial agent is added for primary fermentation, wherein the temperature of the primary fermentation is 28° C. to 30° C., the time is 20 h to 48 h, and the mixture is stirred every 2 h, to obtain a mixture B;

[0041] The composite bacterial agent, calcium chloride and sodium chloride are continuously added to the mixture B for secondary fermentation, and the temperature of the secondary fermentation is 28° C. to 32° C., the time is 1 day to 5 days, and stirring is performed every 2 hours. After the fermentation is completed, corn stalk fermented feed is obtained.

[0042] In one of the embodiments, in the primary fermentation, the added amount of the composite bacterial agent accounts for 1% to 5% of the mass of the base material.

[0043] In one of the embodiments, in the secondary fermentation, the added amount of the composite bacterial agent accounts for 2% to 15% of the mass of the base material.

[0044] In one embodiment, the amount of calcium chloride added is 0.1% to 0.5% of the mass of the base material, and the amount of sodium chloride added is 1% to 7% of the mass of the base material.

[0045] In one embodiment, during the primary fermentation and the secondary fermentation, the humidity is controlled at 30% to 40%.

[0046] By optimizing ingredients and processes in the above scheme, a fermented feed with high nutritional value and good palatability can be obtained as a whole, which is suitable for animal husbandry of cattle and sheep.

[0047] The embodiments of the present invention will be described in detail below with reference to specific examples.

[0048] Embodiment 1:

[0049] A method for preparing corn stalk fermented feed comprises the following steps:

[0050] According to weight, 60 parts of corn stalks are crushed, and then 20 parts of vinegar dregs and 20 parts of bran are added, and mixed evenly to obtain a base material;

[0051] By weight, 10 parts of xylanase, 15 parts of α-glucosidase and 100 parts of liquid carrier (0.1 g / mL glycerol, 0.2 g / mL glucose, 0.01 g / mL dextrin) were mixed to obtain a composite enzyme agent;

[0052] According to weight, 7 parts of Bacillus subtilis, 3 parts of Saccharomyces cerevisiae, 10 parts of Aspergillus niger, 5 parts of sucrose and 75 parts of water were mixed, stirred at 35° C. for 1 min, and then activated at 28° C. for 8 h to obtain a composite bacterial agent;

[0053] Adding a composite enzyme agent in an amount of 9% of the base material by weight to the base material, stirring evenly, performing enzymolysis for 25 hours at a temperature of 42° C. and a pH of 4.8, and then killing the enzyme to obtain a mixture A;

[0054] Add the mixture A to a fermentation kettle, add a composite bacterial agent in an amount of 3% of the mass of the base material for primary fermentation, and the temperature of the primary fermentation is 28° C., the time is 48 hours, the humidity is controlled at 60%, and it is stirred every 2 hours to obtain a mixture B;

[0055] Add 7% of the composite bacterial agent by weight of the base material, 0.3% of the calcium chloride by weight of the base material, and 3% of the sodium chloride by weight of the base material to the mixture B for secondary fermentation. The temperature of the secondary fermentation is 32° C., the time is 4 days, the humidity is controlled at 35%, and the mixture is stirred every 2 hours. After the fermentation is completed, corn stalk fermented feed is obtained.

[0056] Embodiment 2:

[0057] A method for preparing corn stalk fermented feed comprises the following steps:

[0058] According to weight, 50 to 80 parts of corn stalks are crushed, and then 20 to 30 parts of vinegar dregs and 15 to 20 parts of bran are added, and mixed evenly to obtain a base material;

[0059] By weight, 8 parts of xylanase, 17 parts of α-glucosidase and 100 parts of liquid carrier (0.2 g / mL glycerol, 0.4 g / mL glucose, 0.04 g / mL dextrin) were mixed to obtain a composite enzyme agent;

[0060] According to weight, 10 parts of Bacillus subtilis, 3 parts of Saccharomyces cerevisiae, 6 parts of Aspergillus niger, 6 parts of sucrose and 75 parts of water were mixed, stirred at 35° C. for 1 min, and then activated at 28° C. for 8 h to obtain a composite bacterial agent;

[0061] Adding a composite enzyme agent in an amount of 10% of the mass of the base material to the base material, stirring evenly, performing enzymolysis for 25 hours at a temperature of 40° C. and a pH of 4.8, and then killing the enzyme to obtain a mixture A;

[0062] Add the mixture A to a fermentation kettle, add a composite bacterial agent in an amount of 4% of the base material mass for primary fermentation, and the temperature of the primary fermentation is 30° C., the time is 40 hours, the humidity is controlled at 65%, and the mixture is stirred every 2 hours to obtain a mixture B;

[0063] Add 5% of the composite bacterial agent by weight of the base material, 0.4% of the calcium chloride by weight of the base material, and 7% of the sodium chloride by weight of the base material to the mixture B for secondary fermentation. The temperature of the secondary fermentation is 32° C., the time is 5 days, the humidity is controlled at 35%, and the mixture is stirred every 2 hours. After the fermentation is completed, corn stalk fermented feed is obtained.

[0064] Embodiment 3:

[0065] A method for preparing corn stalk fermented feed comprises the following steps:

[0066] According to weight, 60 parts of corn stalks are crushed, and then 22 parts of vinegar dregs and 18 parts of bran are added, and mixed evenly to obtain a base material;

[0067] By weight, 9 parts of xylanase, 16 parts of α-glucosidase and 100 parts of liquid carrier (0.2 g / mL glycerol, 0.1 g / mL glucose, 0.02 g / mL dextrin) were mixed to obtain a composite enzyme agent;

[0068] According to weight, 7 parts of Bacillus subtilis, 5 parts of Saccharomyces cerevisiae, 8 parts of Aspergillus niger, 5 parts of sucrose and 75 parts of water were mixed, stirred at 35° C. for 2 minutes, and then activated at 30° C. for 7 hours to obtain a composite bacterial agent;

[0069] Adding a composite enzyme agent in an amount of 9% of the base material by weight to the base material, stirring evenly, performing enzymolysis for 24 hours at a temperature of 45° C. and a pH of 4.8, and then killing the enzyme to obtain a mixture A;

[0070] The mixture A is added to a fermentation kettle, and a composite bacterial agent is added in an amount of 5% of the mass of the base material for primary fermentation, and the temperature of the primary fermentation is 28° C., the time is 48 hours, the humidity is controlled to 38%, and it is stirred every 2 hours to obtain a mixture B;

[0071] Add 5% of the composite bacterial agent by weight of the base material, 0.4% of the calcium chloride by weight of the base material, and 5% of the sodium chloride by weight of the base material to the mixture B for secondary fermentation. The temperature of the secondary fermentation is 30°C, the time is 3 days, the humidity is controlled at 65%, and the mixture is stirred every 2 hours. After the fermentation is completed, corn stalk fermented feed is obtained.

[0072] Embodiment 4:

[0073] A method for preparing corn stalk fermented feed comprises the following steps:

[0074] According to weight, 65 parts of corn stalks are crushed, and then 20 parts of vinegar dregs and 15 parts of bran are added, and mixed evenly to obtain a base material;

[0075] By weight, 10 parts of xylanase, 15 parts of α-glucosidase and 100 parts of liquid carrier (0.2 g / mL glycerol, 0.5 g / mL glucose, 0.03 g / mL dextrin) were mixed to obtain a composite enzyme agent;

[0076] According to weight, 8 parts of Bacillus subtilis, 5 parts of Saccharomyces cerevisiae, 7 parts of Aspergillus niger, 5 parts of sucrose and 75 parts of water were mixed, stirred at 35° C. for 1 min, and then activated at 28° C. for 8 h to obtain a composite bacterial agent;

[0077] Adding a composite enzyme agent in an amount of 10% of the mass of the base material to the base material, stirring evenly, performing enzymolysis for 25 hours at a temperature of 40° C. and a pH of 4.8, and then killing the enzyme to obtain a mixture A;

[0078] The mixture A is added to a fermentation kettle, and a composite bacterial agent is added in an amount of 5% of the mass of the base material for primary fermentation, and the temperature of the primary fermentation is 28° C., the time is 48 hours, the humidity is controlled at 40%, and it is stirred every 2 hours to obtain a mixture B;

[0079] Add 5% of the composite bacterial agent by weight of the base material, 0.5% of the calcium chloride by weight of the base material, and 5% of the sodium chloride by weight of the base material to the mixture B for secondary fermentation. The temperature of the secondary fermentation is 30°C, the time is 3 days, the humidity is controlled at 65%, and the mixture is stirred every 2 hours. After the fermentation is completed, corn stalk fermented feed is obtained.

[0080] Comparative Example 1:

[0081] The difference between Comparative Example 1 and Example 4 is that in Comparative Example 1, only corn stalks are used for fermentation, and vinegar grains and bran are not added. The rest is the same as Example 4.

[0082] Comparative Example 2:

[0083] The difference between Comparative Example 2 and Example 4 is that no complex enzyme agent is added in Comparative Example 2, and the rest is the same as Example 4.

[0084] Comparative Example 3:

[0085] The difference between Comparative Example 3 and Example 4 is that in Comparative Example 3, only xylanase is added, and the rest is the same as in Example 4.

[0086] Comparative Example 4:

[0087] The difference between Comparative Example 4 and Example 4 is that a single α-glucosidase is added in Comparative Example 4, and the other parts are the same as those in Preliminary Example 4.

[0088] Comparative Example 5:

[0089] The difference between Comparative Example 5 and Example 4 is that a single Bacillus subtilis is added in Comparative Example 5, and the rest is the same as Example 4.

[0090] Comparative Example 6:

[0091] The difference between Comparative Example 6 and Example 4 is that a single brewer's yeast is added in Comparative Example 6, and the rest is the same as Example 4.

[0092] Comparative Example 7:

[0093] The difference between Comparative Example 7 and Example 4 is that a single Aspergillus niger is added in Comparative Example 7, and the rest is the same as Example 4.

[0094] Comparative Example 8:

[0095] The difference between Comparative Example 8 and Example 4 is that Comparative Example 8 does not undergo secondary fermentation, and the rest is the same as Example 4.

[0096] The base material before fermentation, the feed samples of Examples 1 to 4, and the comparative feed samples of Comparative Examples 1 to 8 were analyzed for physical and chemical indicators, wherein the crude protein content was determined with reference to GB / T6432-2018, the crude fiber content was determined with reference to GB / T6434-2006, the crude ash content was determined with reference to GB / T6438-1992, the crude fat content was determined with reference to GB / T6433-2006, the soluble protein content was determined using the Folin-phenol method, and the calcium content was determined with reference to GB / T5009.87-2016. The results are shown in Table 1 below.

[0097] Table 1: Physical and chemical indicators

[0098]

[0099]

[0100] From the data analysis of Table 1, it can be seen that the corn stalk fermented feed obtained by the present invention has a high nutritional value. The difference between Comparative Example 1 and Example 4 is that no vinegar residue and bran are added in Comparative Example 1, but it can be seen that it can affect the total nutritional value of the fermented feed; Comparative Examples 2 to 4 change the composition of the composite enzyme agent, but it is seen that there is a significant effect on the fermentation effect, indicating that the composite optimization of the enzyme components in this application has a positive effect on the fermentation effect; Comparative Examples 5 to 7 change the composition of the composite enzyme agent, but the fermentation effect is obviously worse than that of Example 4, indicating that the present application can achieve a better fermentation effect through the compounded strains; The difference between Comparative Example 8 and Example 4 is that no secondary fermentation is performed in Comparative Example 8, but it is seen that the nutritional value of the fermented feed is worse than that of Example 4, indicating that the optimization of the process of this application can also promote the fermentation effect. On the whole, this application can effectively utilize corn stalks through the optimization of components and processes, and interact with each other to obtain fermented feed with significant fermentation effect.

[0101] The use effect of the corn stalk fermented feed of the present invention is further described below in conjunction with a feeding experiment.

[0102] The corn stalk fermented feed prepared in Example 4 was further fed to the test. 20 Nandan cattle in good health and weighing 200±15kg were selected and randomly divided into 4 groups, 5 replicates in each group, and 1 cattle in each replicate. The pre-test period was 10 days, the main test period was 90 days, and the feeding conditions of each group were shown in Table 2 (dry matter basis, %).

[0103] Feeding management: Clean and disinfect the experimental cattle shed before the experiment. The experimental cattle were separated by groups and tied up in rows in the same cattle shed. They were fed twice a day at 7:00 and 18:00 respectively. There was no limit on the amount of feeding each time. They were free to drink water. The trough was cleaned once before feeding in the morning every day, and the amount of residual feed was recorded. The trough was brushed once every 7 days, the cattle shed was cleaned once every 15 days, and disinfected once every 30 days to prevent the occurrence of infectious diseases.

[0104] Growth performance index determination: Record the amount of each feeding in units of repetitions, weigh the amount of leftover feed before feeding every morning, and calculate the average daily feed intake (ADFI) of each cow. The fasting body weight before feeding on the first day of the experiment was taken as the initial body weight, and the fasting body weight after the end of the experiment was taken as the final body weight, and the average daily weight gain (ADG) and feed-to-weight ratio (F / G) were calculated. The results are shown in Table 3.

[0105] Serum indicators: Serum superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) were measured according to the kit instructions. Immunoglobulins (IgG, IgM, IgA) were subjected to enzyme-linked immunosorbent assay (ELISA) under a clean bench, and the results are shown in Table 4.

[0106] Table 2: Diet (dry matter basis, %)

[0107]

[0108] It should be noted that the basic diet in Table 2 is obtained by mixing the following ingredients: 30% hay + 50% sugarcane tail silage + 20% concentrated feed.

[0109] Table 3: Growth performance indicators

[0110]

[0111]

[0112] From the analysis of the growth performance indicators in Table 3, it can be seen that the corn stalk fermented feed of the present application has a higher conversion rate and has a better growth-promoting effect.

[0113] Table 4: Serum immune indexes

[0114]

[0115] From the data analysis in Table 4, it can be seen that the immunity of animals can be effectively improved by feeding the corn stalk fermented feed of the present application.

[0116] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0117] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A corn stalk fermented feed, characterized in that: The corn stalk fermented feed is obtained by treating the base material with a composite enzyme agent and a composite bacterial agent; The base material comprises the following raw materials in parts by weight: 50 to 80 parts of corn stalks, 20 to 30 parts of vinegar lees, and 15 to 20 parts of bran; The composite enzyme preparation is composed of xylanase, α-glucosidase and a liquid carrier, wherein the enzyme activity of the xylanase is 1000u / g to 1200u / g, and the enzyme activity of the α-glucosidase is 800u / g to 1200u / g; The composite bacterial agent is obtained by activation treatment of raw materials including 7 to 10 parts of Bacillus subtilis, 3 to 5 parts of brewer's yeast, 5 to 10 parts of Aspergillus niger, 5 to 7 parts of sucrose and 60 to 85 parts of water.

2. The corn stalk fermented feed according to claim 1, characterized in that: The liquid carrier comprises the following components: 0.1 g / mL to 0.2 g / mL of glycerol, 0.1 g / mL to 0.5 g / mL of glucose, and 0.01 g / mL to 0.05 g / mL of dextrin.

3. The corn stalk fermented feed according to claim 1, characterized in that: The activation treatment comprises mixing Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus niger, sucrose and water, stirring at 35°C to 38°C for 1min to 2min, and then activating at 28°C to 35°C for 5h to 8h to obtain a composite bacterial agent.

4. The corn stalk fermented feed according to claim 1, characterized in that: The added amount of the complex enzyme agent accounts for 1% to 10% of the mass of the base material.

5. The corn stalk fermented feed according to claim 1, characterized in that: The added amount of the composite bacterial agent accounts for 1% to 10% of the mass of the base material.

6. A method for preparing corn stalk fermented feed, characterized in that: The preparation method is used to prepare the corn stalk fermented feed according to any one of claims 1 to 5, and the preparation method comprises the following steps: The corn stalks are crushed, and then vinegar dregs and bran are added and mixed evenly to obtain a base material; Add the composite enzyme to the base material, stir evenly, and perform enzymolysis for 20 h to 25 h at a temperature of 40° C. to 55° C. and a pH of 4.5 to 6.0, and then inactivate the enzyme to obtain a mixture A; The mixture A is added into a fermentation kettle, and a composite bacterial agent is added for primary fermentation, wherein the temperature of the primary fermentation is 28° C. to 30° C., the time is 20 h to 48 h, and the mixture is stirred every 2 h, to obtain a mixture B; The composite bacterial agent, calcium chloride and sodium chloride are continuously added to the mixture B for secondary fermentation, and the temperature of the secondary fermentation is 28° C. to 32° C., the time is 1 day to 5 days, and stirring is performed every 2 hours. After the fermentation is completed, corn stalk fermented feed is obtained.

7. The preparation method according to claim 6, characterized in that: In the primary fermentation, the added amount of the composite bacterial agent accounts for 1% to 5% of the mass of the base material.

8. The preparation method according to claim 6, characterized in that: In the secondary fermentation, the added amount of the composite bacterial agent accounts for 2% to 15% of the mass of the base material.

9. The preparation method according to claim 6, characterized in that: The added amount of the calcium chloride accounts for 0.1% to 0.5% of the mass of the base material, and the added amount of the sodium chloride accounts for 1% to 7% of the mass of the base material.

10. The preparation method according to claim 6, characterized in that: During the primary fermentation and the secondary fermentation, the humidity is controlled at 30% to 40%.

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