High-food-calling feed and production process thereof

Abstract

The invention discloses a high-food-calling feed and a production process thereof, and belongs to the technical field of feed production, and the high-food-calling feed comprises the following raw materials in parts by mass: 27-35 parts of corn flour, 22-25 parts of pea meal, 17-23 parts of a phagostimulant, 2-3 parts of fish oil, 1-2 parts of soybean oil, 0.5-1.0 part of a microbial agent and 0.01-0.10 part of multivitamins. A self-made phagostimulant is added, specifically, the phagostimulant prepared by the preparation method disclosed by the invention is prepared by performing hydrolytic hydrolysis on tuna bones through animal protein and then compounding modified chitosan (carboxymethyl chitosan carrying ciprofloxacin), and finally, through detection, the phagostimulant has the advantages that the content of the prepared phagostimulant in the tuna bones can be greatly improved; by adding the phagostimulant prepared by the invention, the phagostimulant and the antibacterial property of the feed can be remarkably improved.

Description

Technical Field

[0001] The invention belongs to the technical field of feed production, and in particular relates to a highly appetizing feed and a production process thereof. Background Art

[0002] In aquaculture, the attractivity of feed is crucial, and improving the attractivity of feed is necessary in many aspects. First of all, good attractivity helps to increase the food intake of fish and shrimp. Fish and shrimp have a wealth of food choices in the natural environment, and if artificial feed lacks sufficient appeal, fish and shrimp may lose their appetite and lead to insufficient food intake; secondly, improving the attractivity can reduce feed waste. When the attractivity of feed is poor, fish and shrimp may be picky or only eat part of the feed, and the remaining feed will sink to the bottom of the water, causing waste, which not only increases the cost of breeding, but also may pollute the water quality; furthermore, for fish and shrimp in the larval stage or just after transportation, feed with good attractivity is more critical. Improving the attractivity of feed can help them open their mouths to eat smoothly, laying the foundation for subsequent growth and development.

[0003] In short, improving the attractant of feed is an important link in aquaculture that cannot be ignored. It plays a vital role in increasing the food intake of fish and shrimp, reducing feed waste, promoting the growth and development of fish and shrimp, and improving aquaculture efficiency. Summary of the invention

[0004] The invention provides a feed and a production process thereof, and aims to improve the appetitiveness of the finally produced feed by adding a homemade attractant to a traditional feed.

[0005] The purpose of the present invention can be achieved through the following technical solutions: A highly appetizing feed, comprising the following raw materials in parts by weight: 27-35 parts corn flour, 22-25 parts pea flour, 17-23 parts attractant, 2-3 parts fish oil, 1-2 parts soybean oil, 0.5-1.0 parts bacterial agent and 0.01-0.10 parts multivitamins; The phagostimulant is prepared by the following steps: Wash and dry tuna bones and grind them through a 200-mesh sieve to obtain tuna bone powder, add water to the tuna bone powder at a mass ratio of 1:1 to obtain an enzymatic precursor, add animal protein hydrolase thereto, use acetic acid to adjust the pH of the system to 6.7-7.0, after the adjustment is completed, heat the system to 53-55°C, and perform constant temperature enzymolysis for 8-10 hours, after the enzymolysis is completed, heat the system to 90°C to inactivate the enzyme for 30 minutes to obtain an enzymatic product, add modified chitosan to the enzymatic product, mix and stir for 2-3 hours, and then dry to constant weight at room temperature to obtain an attractant.

[0006] Furthermore, the amount of the animal protein hydrolase used is 2.5-3.0wt% of the enzymatic precursor.

[0007] Furthermore, the amount of the modified chitosan is 14-15wt% of the enzymatic hydrolysis product.

[0008] Furthermore, the modified chitosan is prepared by the following steps: Ciprofloxacin, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide are added to dichloromethane. After the addition is completed, the system is heated to 35-40°C and stirred at a constant temperature for 20-30 minutes. Carboxymethyl chitosan is then added thereto. After the addition is completed, the system is heated to 45-50°C and stirred at a constant temperature for 12 hours. After the reaction is completed, the system is cooled to room temperature, and dichloromethane is removed by rotary evaporation to obtain modified chitosan.

[0009] Furthermore, the usage ratio of dichloromethane, ciprofloxacin, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, N-hydroxysuccinimide and carboxymethyl chitosan is 200mL: 0.2-0.22mol: 18-20g: 14-15g: 21-22g.

[0010] Furthermore, the bacterial agent is prepared by mixing Bacillus subtilis powder, lactic acid bacteria powder and yeast powder in a mass ratio of 2:2:1 to 2.

[0011] Furthermore, the effective live bacteria content in the Bacillus subtilis powder, lactic acid bacteria powder and yeast powder is 3×10 8 cfu / g~3×10 9 cfu / g.

[0012] Furthermore, the complex vitamin is formed by mixing water-soluble vitamins and fat-soluble vitamins in a mass ratio of 2:1.

[0013] Furthermore, the water-soluble vitamins are formed by mixing vitamin B1, vitamin B2 and folic acid in a mass ratio of 1:1:1.

[0014] Furthermore, the fat-soluble vitamins are formed by mixing vitamin A, vitamin D and vitamin E in a mass ratio of 2 to 3:2:1.

[0015] Furthermore, the production process of the highly appetizing feed comprises the following steps: The above raw materials are weighed according to mass parts. First, corn flour and pea flour are mixed for 20 to 30 minutes. Then, they are ultrafinely ground and passed through a 200-mesh sieve. Then, fish oil, soybean oil, bacterial agent and multivitamin are added thereto. The mixture is stirred and mixed for 20 to 30 minutes to obtain a mixture. Then, attractants are stirred and mixed for 20 to 30 minutes to obtain feed powder. Then, 1.5 to 2.0 wt% of pure water is stirred and added to the feed powder. The mixture is stirred for 30 minutes. Then, the mixture is placed in a granulator to form particles with a particle size of 1.2 to 2.0 mm. The particles are dried at 30 to 40°C for 2 to 5 hours to obtain a highly appetizing feed.

[0016] Beneficial effects of the present invention: The present invention provides a highly appetizing feed, comprising corn flour, pea flour, an appetizer, fish oil, soybean oil, a bacterial agent and a compound vitamin. The present invention improves the appetizing property of the feed by adding a homemade appetizer. Meanwhile, according to detection, the addition of the appetizer significantly improves the antibacterial property of the feed. The specific principle is as follows: The attractant prepared by the present invention is prepared by hydrolyzing tuna bones with animal protein and then compounding modified chitosan (carboxymethyl chitosan loaded with ciprofloxacin). Tuna bones can release a variety of peptides and a variety of umami amino acids that are highly attractive to fish and shrimp after enzymolysis, and the utilization rate of nutrients after enzymolysis is higher. However, when the enzymolysis product obtained by enzymolysis of tuna bones is directly added to feed, there are problems that the enzymolysis product is easy to lose and the compatibility between the enzymolysis product and feed is poor. Therefore, the present invention adds homemade modified chitosan thereto.

[0017] First, the modified chitosan prepared by the present invention is prepared by carboxymethyl chitosan carrying ciprofloxacin. First, carboxymethyl chitosan has abundant carboxyl and amino functional groups on its surface, and the carboxyl and amino functional groups can form hydrogen bonds with abundant hydroxyl groups in the tuna bone hydrolysate, thereby improving the interaction between the hydrolysates. At the same time, in the subsequent process of mixing and granulating the feed powder with water, the carboxymethyl chitosan containing abundant carboxyl and amino functional groups can improve the bonding performance between the attractant and the feed substrates such as corn flour and pea flour, that is, the carboxymethyl chitosan itself also has excellent bonding performance, which helps to improve the uniformity and durability of the attractant of the finally prepared feed. In addition, since carboxymethyl chitosan has excellent film-forming properties, the addition of carboxymethyl chitosan can reduce the loss of the hydrolysate in the subsequent feed preparation process, and reduce the occurrence of the disintegration phenomenon of the subsequent feed after storage and application into the water body. The addition of carboxymethyl chitosan, by reducing the loss of the hydrolysate and improving the compatibility between the hydrolysate and the feed, is ultimately manifested in improving the attractant of the feed.

[0018] Then, carboxymethyl chitosan, as a natural polysaccharide, can improve the palatability of feed and has certain nutritional value itself.

[0019] Finally, carboxymethyl chitosan has excellent antibacterial properties. The macromolecular chain of carboxymethyl chitosan itself is positively charged, and can affect the normal activities of bacteria through electrostatic adsorption. At the same time, carboxymethyl chitosan can penetrate the cell wall and cell membrane of bacteria to hinder bacterial reproduction. Further, the present invention further carries ciprofloxacin on the surface of carboxymethyl chitosan. Ciprofloxacin mainly inhibits bacterial DNA gyrase and topoisomerase IV, interferes with bacterial DNA replication and transcription process to achieve antibacterial effect. Carboxymethyl chitosan can damage bacterial cell wall or change the permeability of cell membrane, so that ciprofloxacin can enter the interior of bacteria more easily, exert its effect of inhibiting bacterial DNA gyrase, thereby blocking bacterial DNA replication and transcription, and achieving synergistic antibacterial effect. DETAILED DESCRIPTION

[0020] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0021] Example 1

[0022] Preparation of attractant: (1) Preparation of modified chitosan: 200 mL of dichloromethane (AR; ≥99.5%; purchased from Sinopharm Chemical Reagent Co., Ltd.) was added thereto, 0.2 mol of ciprofloxacin (98%; purchased from Sinopharm Chemical Reagent Co., Ltd.), 18 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (98%; purchased from Sinopharm Chemical Reagent Co., Ltd.) and 14 g of N-hydroxysuccinimide (98%; purchased from Sinopharm Chemical Reagent Co., Ltd.) were added thereto. After the addition was completed, the system was heated to 35° C. and stirred at constant temperature for 20 min. Then, 21 g of carboxymethyl chitosan (degree of substitution ≥80%; purchased from Sinopharm Chemical Reagent Co., Ltd.) was added thereto. After the addition was completed, the system was heated to 45° C. and stirred at constant temperature for 12 h. After the reaction was completed, the system was cooled to room temperature and dichloromethane was removed by rotary evaporation to obtain modified chitosan. (2) Preparation of attractant: Take tuna bones (purchased from Zhejiang Rongchuang Food Industry Co., Ltd.), rinse them three times with clean water, dry them at 40°C to constant weight, grind them through a 200-mesh sieve to obtain tuna bone powder, add water to the tuna bone powder at a mass ratio of 1:1 to obtain an enzymatic precursor, add 2.5wt% of animal protein hydrolase (300,000 u / g; purchased from Nanning Dongheng Huadao Biotechnology Co., Ltd.) to it, use acetic acid (99.5%; purchased from Shanghai MacLean Biochemical Technology Co., Ltd.) to adjust the pH of the system to 6.7, after the adjustment is completed, heat the system to 53°C, and perform enzymolysis at a constant temperature for 8 hours, after the enzymolysis is completed, heat the system to 90°C to inactivate the enzyme for 30 minutes to obtain an enzymatic product, add 14wt% of modified chitosan to the enzymatic product, mix and stir for 2 hours, and then dry it at room temperature to constant weight to obtain an attractant.

[0023] Example 2

[0024] Preparation of attractant: (1) Preparation of modified chitosan: 200 mL of dichloromethane (AR; ≥99.5%; purchased from Sinopharm Chemical Reagent Co., Ltd.) was added thereto, 0.22 mol of ciprofloxacin (98%; purchased from Sinopharm Chemical Reagent Co., Ltd.), 20 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (98%; purchased from Sinopharm Chemical Reagent Co., Ltd.) and 15 g of N-hydroxysuccinimide (98%; purchased from Sinopharm Chemical Reagent Co., Ltd.) were added thereto. After the addition was completed, the system was heated to 38° C. and stirred at constant temperature for 30 min. Then, 22 g of carboxymethyl chitosan (substitution degree ≥80%; purchased from Sinopharm Chemical Reagent Co., Ltd.) was added thereto. After the addition was completed, the system was heated to 48° C. and stirred at constant temperature for 12 h. After the reaction was completed, the system was cooled to room temperature and dichloromethane was removed by rotary evaporation to obtain modified chitosan. (2) Preparation of attractant: Take tuna bones (purchased from Zhejiang Rongchuang Food Industry Co., Ltd.), rinse them three times with clean water, dry them at 45°C to constant weight, grind them through a 200-mesh sieve to obtain tuna bone powder, add water to the tuna bone powder at a mass ratio of 1:1 to obtain an enzymatic precursor, add 3.0wt% of animal protein hydrolase (300,000 u / g; purchased from Nanning Dongheng Huadao Biotechnology Co., Ltd.) to it, use acetic acid (99.5%; purchased from Shanghai MacLean Biochemical Technology Co., Ltd.) to adjust the pH of the system to 6.8, after the adjustment is completed, heat the system to 54°C, and perform enzymolysis at a constant temperature for 10 hours. After the enzymolysis is completed, heat the system to 90°C to inactivate the enzyme for 30 minutes to obtain an enzymatic product, add 15wt% of modified chitosan to the enzymatic product, mix and stir for 3 hours, and then dry it at room temperature to constant weight to obtain an attractant.

[0025] Example 3

[0026] Preparation of attractant: (1) Preparation of modified chitosan: 200 mL of dichloromethane (AR; ≥99.5%; purchased from Sinopharm Chemical Reagent Co., Ltd.) was added thereto, 0.22 mol of ciprofloxacin (98%; purchased from Sinopharm Chemical Reagent Co., Ltd.), 20 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (98%; purchased from Sinopharm Chemical Reagent Co., Ltd.) and 15 g of N-hydroxysuccinimide (98%; purchased from Sinopharm Chemical Reagent Co., Ltd.) were added thereto. After the addition was completed, the system was heated to 40°C and stirred at constant temperature for 30 min. Then, 22 g of carboxymethyl chitosan (substitution degree ≥80%; purchased from Sinopharm Chemical Reagent Co., Ltd.) was added thereto. After the addition was completed, the system was heated to 50°C and stirred at constant temperature for 12 h. After the reaction was completed, the system was cooled to room temperature and dichloromethane was removed by rotary evaporation to obtain modified chitosan. (2) Preparation of attractant: Take tuna bones (purchased from Zhejiang Rongchuang Food Industry Co., Ltd.), rinse them three times with clean water, dry them at 50°C to constant weight, grind them through a 200-mesh sieve to obtain tuna bone powder, add water to the tuna bone powder at a mass ratio of 1:1 to obtain an enzymatic precursor, add 3.0wt% of animal protein hydrolase (300,000 u / g; purchased from Nanning Dongheng Huadao Biotechnology Co., Ltd.) to it, use acetic acid (99.5%; purchased from Shanghai MacLean Biochemical Technology Co., Ltd.) to adjust the pH of the system to 7.0, after the adjustment is completed, heat the system to 55°C, and perform enzymolysis at a constant temperature for 10 hours. After the enzymolysis is completed, heat the system to 90°C to inactivate the enzyme for 30 minutes to obtain an enzymatic product, add 15wt% of modified chitosan to the enzymatic product, mix and stir for 3 hours, and then dry it at room temperature to constant weight to obtain an attractant.

[0027] Comparative Example 1 Comparative Example 1 is the control group of Example 2, in which the modified chitosan used in preparing the attractant in Example 2 is replaced with chitosan, that is, the process of carrying ciprofloxacin is removed, and the remaining raw materials, raw material amounts and preparation steps are kept the same as in Example 2, and finally an attractant is obtained.

[0028] Comparative Example 2 Comparative Example 2 is the control group of Example 2, in which the modified chitosan in the attractant prepared in Example 2 is replaced and removed, and the remaining raw materials, raw material amounts and preparation steps remain the same as in Example 2, and finally an attractant is obtained.

[0029] Example 4

[0030] A highly appetizing feed: First, a highly appetizing feed comprises the following raw materials by weight: 27 parts of corn flour, 22 parts of pea flour, 17 parts of the appetizer prepared in Example 1, 2 parts of fish oil, 1 part of soybean oil, 0.5 parts of bacterial agent and 0.01 parts of multivitamins; Then, the production process of highly appetizing feed includes the following steps: The above raw materials are weighed according to mass parts. First, corn flour and pea flour are mixed for 20 minutes, and then ultrafinely ground and passed through a 200-mesh sieve. Fish oil, soybean oil, bacterial agent and multivitamin are added thereto, and the mixture is stirred for 20 minutes to obtain a mixture. The attractant prepared in Example 1 is stirred into the mixture, and the mixture is stirred for 20 minutes to obtain a feed powder. 1.5 wt% of pure water is stirred into the feed powder, and the mixture is mixed for 30 minutes. The mixture is placed in a granulator to form particles with a particle size of 2.0 mm, and dried at 40°C for 2 hours to obtain a highly appetizing feed.

[0031] Example 5

[0032] A highly appetizing feed: First, a highly appetizing feed comprises the following raw materials by weight: 32 parts of corn flour, 24 parts of pea flour, 22 parts of the appetizer prepared in Example 2, 2 parts of fish oil, 2 parts of soybean oil, 0.5 parts of bacterial agent and 0.05 parts of multivitamins; Then, the production process of highly appetizing feed includes the following steps: The above raw materials are weighed according to mass parts. First, corn flour and pea flour are mixed for 30 minutes, and then ultrafinely ground and passed through a 200-mesh sieve. Fish oil, soybean oil, bacterial agent and multivitamin are added thereto, and stirred for 30 minutes to obtain a mixture. The attractant prepared in Example 2 is stirred into the mixture, and the mixture is stirred for 30 minutes to obtain feed powder. 2.0wt% of pure water is stirred into the feed powder, and the mixture is mixed for 30 minutes. The mixture is placed in a granulator to form particles with a particle size of 1.2 mm, and dried at 30°C for 5 hours to obtain a highly appetizing feed.

[0033] Example 6

[0034] A highly appetizing feed: First, a highly appetizing feed comprises the following raw materials by weight: 35 parts of corn flour, 25 parts of pea flour, 23 parts of the appetizer prepared in Example 3, 3 parts of fish oil, 2 parts of soybean oil, 1.0 part of bacterial agent and 0.10 part of multivitamins; Then, the production process of highly appetizing feed includes the following steps: The above raw materials are weighed according to mass parts. First, corn flour and pea flour are mixed for 30 minutes, and then ultrafinely ground and passed through a 200-mesh sieve. Fish oil, soybean oil, bacterial agent and multivitamin are added thereto, and stirred for 30 minutes to obtain a mixture. The attractant prepared in Example 3 is stirred into the mixture, and the mixture is stirred for 30 minutes to obtain feed powder. 2.0wt% of pure water is stirred into the feed powder, and the mixture is mixed for 30 minutes. The mixture is placed in a granulator to form particles with a particle size of 2.0mm, and dried at 30°C for 5 hours to obtain a highly appetizing feed.

[0035] Comparative Example 3 Comparative Example 3 is the control group of Example 5, in which the attractant prepared in Example 2 of Example 5 is replaced by the attractant prepared in Comparative Example 1, and the remaining raw materials, raw material amounts and production process remain the same as those in Example 5, and finally a highly appetizing feed is obtained.

[0036] Comparative Example 4 Comparative Example 4 is the control group of Example 5, in which the attractant prepared in Example 2 in Example 5 is replaced by the attractant prepared in Comparative Example 2, and the remaining raw materials, raw material amounts and production process remain the same as in Example 5, and finally a highly appetizing feed is obtained.

[0037] Comparative Example 5 Comparative Example 5 is the control group of Example 5, in which the attractant prepared in Example 2 in Example 5 is deleted, and the remaining raw materials, raw material amounts and production process are kept the same as those in Example 5 to obtain feed.

[0038] Test Example 1 The feeds prepared in Examples 4 to 6 and Comparative Examples 3 to 5 were subjected to performance tests. The performance test process is as follows, and the test results are shown in Table 1: Appetite: The relative biting rate and relative appetitive rate of the feeds prepared in Examples 4 to 6 and Comparative Examples 3 to 5 were determined with reference to the test method in the existing document "Study on the Appetitiveness of 10 Common Feed Raw Materials on Yellow River Carp". The relative biting rate was determined with reference to the billiard method in the document, and the relative appetitive rate was determined with reference to the maze method in the document, as follows: Experimental fish and shrimp: grass carp with a body length of 5-7 cm and a body weight of 4-5 g; whiteleg shrimp with a body length of 2-3 cm and a body weight of 0.3-0.4 g.

[0039] Billiard ball method: Before the test, weigh 2g of feed and flour, wet them with water and rub them into balls, then wrap them with 2cm×2cm×0.5cm cotton and then with a single layer of 4cm×4cm medical gauze, record them as test balls (feed balls) and control balls (flour balls), hang the test balls and control balls in a glass tank (50cm long×40cm wide×30cm high) at a depth of 15cm, with a distance of 20cm between the two balls. There are 10 grass carps / 10 white shrimps in the glass tank. The test records the number of times the grass carps / white shrimps peck at the two balls within 10 minutes. The test is carried out once a day at 09:00 in the morning and 16:00 in the afternoon. The positions of the test balls and control balls are swapped in the afternoon test. The entire test process is recorded by a camera above the test tank. The relative biting rate (RV) is calculated by the following formula: RV=(N t -N c ) / (N t +N c )×100%; N t : Number of times of pecking the test ball; N c : Number of times the control ball was pecked.

[0040] Relative feeding rate: Before the test, weigh 2g of feed and flour, wet them with water and roll them into balls, then wrap them with 2cm×2cm×0.5cm cotton and then with a single layer of 4cm×4cm medical gauze, record them as test balls (feed balls) and control balls (flour balls). Refer to the literature "Study on the Feeding Attraction of Yellow River Carp with 10 Common Feed Raw Materials", and build a test maze. The test maze consists of a glass box with a length of 100cm×width of 50cm×height of 30cm. The glass box is divided into area A, area A', area B, area B' and area C by partitions. Among them, 10 grass carps / 10 white shrimps are placed in area C. Test balls and control balls are placed in areas A and A' respectively. Area A is connected to area C through area B. Similarly, area A' is connected to area C through area B'. For details, please refer to the existing literature. This experiment is designed in full reference to the existing technology to ensure the reliability and repeatability of the test results. The relative rate of attraction (RFI) is calculated by the following formula: RFI=(TDI-BDI) / (TDI+BDI)×100%; TDI: number of experimental fish / shrimp entering zone A; BDI: The number of experimental fish / shrimp entering the A` zone.

[0041] Antibacterial performance: 0.1 g of the feed prepared in Examples 4 to 6 and Comparative Examples 3 to 5 was mixed with 10 mL of 1×10 7The mixture of Escherichia coli liquid and Staphylococcus aureus liquid was placed in a test tube and placed on a constant temperature shaker at 37°C and 120 r / min for 4 h. This was used as the experimental group, and the unmixed feed was used as the blank group. The bacterial liquid of the experimental group and the blank group was taken out and diluted to 1×10 5 CFU / mL, 100 μL of bacterial solution was spread on the surface of the agar plate, and incubated in an oscillating incubator at room temperature for 24 hours. The antibacterial rate was calculated by the plate colony counting method: Antibacterial rate (%) = [(N a -N b ) / Na]×100%; N a : The number of colonies in the blank group; N b : The colony counts of the experimental group.

[0042] Table 1 Test results

[0043] It can be seen from Table 1 that the feeds prepared in Examples 4 to 6 of the present invention have excellent feeding properties for fish and shrimp, and have excellent antibacterial properties against Escherichia coli and Staphylococcus aureus.

[0044] Specifically, by comparing the data of Example 5 and Comparative Example 3, it can be obtained that the present invention significantly improves the antibacterial property (against Escherichia coli and Staphylococcus aureus) of the finally prepared feed by carrying ciprofloxacin on the surface of carboxymethyl chitosan.

[0045] Specifically, by comparing the data of Example 5 and Comparative Example 4, it can be obtained that the present invention significantly improves the antibacterial property (against Escherichia coli and Staphylococcus aureus) of the final prepared feed by adding modified chitosan, and at the same time helps to improve the feeding attractivity of the feed to fish and shrimp.

[0046] Specifically, by comparing the data of Example 5 and Comparative Example 5, it can be obtained that the present invention significantly improves the attractant and antibacterial performance (against Escherichia coli and Staphylococcus aureus) of the feed for fish and shrimp by adding attractants.

[0047] It should be noted that, in this article, terms such as "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or equipment that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or equipment.

[0048] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A highly appetizing feed, characterized in that: Including the following raw materials by weight: 27-35 parts corn flour, 22-25 parts pea flour, 17-23 parts attractant, 2-3 parts fish oil, 1-2 parts soybean oil, 0.5-1.0 parts bacterial agent and 0.01-0.10 parts multivitamins; The attractant is prepared by the following steps: Wash and dry tuna bones and grind them through a 200-mesh sieve to obtain tuna bone powder, add water to the tuna bone powder at a mass ratio of 1:1 to obtain an enzymatic precursor, add animal protein hydrolase thereto, use acetic acid to adjust the pH of the system to 6.7-7.0, after the adjustment is completed, heat the system to 53-55°C, and perform constant temperature enzymolysis for 8-10 hours, after the enzymolysis is completed, heat the system to 90°C to inactivate the enzyme for 30 minutes to obtain an enzymatic product, add modified chitosan to the enzymatic product, mix and stir for 2-3 hours, and then dry to constant weight at room temperature to obtain an attractant.

2. A highly appetizing feed according to claim 1, characterized in that: The amount of the animal protein hydrolyzing enzyme used is 2.5-3.0 wt % of the enzymatic precursor.

3. A highly appetizing feed according to claim 1, characterized in that: The amount of the modified chitosan is 14-15 wt % of the enzymatic hydrolysate.

4. A highly appetizing feed according to claim 1, characterized in that: The modified chitosan is prepared by the following steps: Ciprofloxacin, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide are added to dichloromethane. After the addition is completed, the system is heated to 35-40°C and stirred at a constant temperature for 20-30 minutes. Carboxymethyl chitosan is then added thereto. After the addition is completed, the system is heated to 45-50°C and stirred at a constant temperature for 12 hours. After the reaction is completed, the system is cooled to room temperature, and dichloromethane is removed by rotary evaporation to obtain modified chitosan.

5. A highly appetizing feed according to claim 4, characterized in that: The usage ratio of the dichloromethane, ciprofloxacin, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, N-hydroxysuccinimide and carboxymethyl chitosan is 200 mL: 0.2-0.22 mol: 18-20 g: 14-15 g: 21-22 g.

6. A highly appetizing feed according to claim 1, characterized in that: The bacterial agent is prepared by mixing Bacillus subtilis powder, lactic acid bacteria powder and yeast powder in a mass ratio of 2:2:1-2.

7. A highly appetizing feed according to claim 6, characterized in that: The effective live bacteria content in the Bacillus subtilis powder, lactic acid bacteria powder and yeast powder is 3×10 8 cfu / g~3×10 9 cfu / g.

8. A highly appetizing feed according to claim 1, characterized in that: The complex vitamin is prepared by mixing water-soluble vitamins and fat-soluble vitamins in a mass ratio of 2:

1.

9. A highly appetizing feed according to claim 8, characterized in that: The water-soluble vitamins are formed by mixing vitamin B1, vitamin B2 and folic acid in a mass ratio of 1:1:1; the fat-soluble vitamins are formed by mixing vitamin A, vitamin D and vitamin E in a mass ratio of 2 to 3:2:

1.

10. A production process for a highly appetizing feed according to any one of claims 1 to 9, characterized in that: The following steps are involved: The above raw materials are weighed according to mass parts. First, corn flour and pea flour are mixed for 20 to 30 minutes. Then, they are ultrafinely ground and passed through a 200-mesh sieve. Then, fish oil, soybean oil, bacterial agent and multivitamin are added thereto. The mixture is stirred and mixed for 20 to 30 minutes to obtain a mixture. Then, attractants are stirred and mixed for 20 to 30 minutes to obtain feed powder. Then, 1.5 to 2.0 wt% of pure water is stirred and added to the feed powder. The mixture is stirred for 30 minutes. Then, the mixture is placed in a granulator to form particles with a particle size of 1.2 to 2.0 mm. The particles are dried at 30 to 40°C for 2 to 5 hours to obtain a highly appetizing feed.

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