Feed for preventing and treating porcine parvovirus disease and preparation method thereof

Abstract

The invention relates to the technical field of animal feeds, and discloses a feed for preventing and treating porcine parvovirus diseases and a preparation method of the feed. The invention discloses a feed for preventing and treating porcine parvovirus diseases. The feed comprises the following raw materials in parts by mass: 40-60 parts of corn, 15-25 parts of wheat, 10-20 parts of wheat bran, 5-10 parts of bean dregs, 4-6 parts of fish meal, 2-3 parts of table salt, 3-5 parts of a traditional Chinese medicine additive and 6-8 parts of a nutrition additive. The feed for preventing and treating the porcine parvovirus disease, provided by the invention, has a good prevention and treatment effect on the porcine parvovirus disease.

Description

Technical Field

[0001] The invention relates to the technical field of animal feed, and in particular to a feed for preventing and treating porcine parvovirus disease and a preparation method thereof. Background Art

[0002] Porcine parvovirus disease (PPVD) is a reproductive disorder in pigs caused by porcine parvovirus (PPV), which is mainly manifested by abortion, stillbirth, deformed fetuses, mummified fetuses, weak piglets, and repeated infertility in infected sows. Currently, porcine parvovirus disease is widely distributed around the world and is endemic in most infected pig farms. Once a pig herd is infected, it is difficult to completely eliminate it. Therefore, porcine parvovirus disease seriously restricts the development of the pig industry.

[0003] In the prior art, the prevention of porcine parvovirus disease mainly relies on immune prevention. However, not all vaccinated pigs can produce an effective immune response. Some pigs may fail to be immuned due to individual differences or other factors. In addition, porcine parvovirus is very easy to mutate, and existing vaccines may not provide effective immune prevention. In addition, vaccination requires a certain cost. For pig farms with limited resources, vaccination may become an economic burden. For the treatment of porcine parvovirus disease, commonly used drugs are antibiotics such as doxycycline and broad-spectrum antiviral drugs such as ribavirin. Among them, antibiotics can produce drug resistance and cause damage to the liver and kidneys, and long-term and large-scale use of broad-spectrum antiviral drugs can also cause damage to the heart and kidneys. Therefore, how to prevent and treat porcine parvovirus disease with high efficiency, low toxicity and side effects, and low cost has become a hot topic in this field.

[0004] Pig feed is an indispensable part of the pig breeding process and plays a vital role in the growth, development and health of pigs. If pig feed is given certain functions to prevent and treat porcine parvovirus disease, it is expected to achieve high-efficiency, low-toxicity and low-cost prevention and treatment of porcine parvovirus disease. Summary of the invention

[0005] The purpose of the present invention is to provide a feed for preventing and treating porcine parvovirus disease and a preparation method thereof. The feed for preventing and treating porcine parvovirus disease has a certain preventive and therapeutic function on porcine parvovirus disease.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] One of the technical solutions of the present invention:

[0008] A feed for preventing and treating porcine parvovirus disease comprises the following raw materials in parts by weight: 40-60 parts of corn, 15-25 parts of wheat, 10-20 parts of wheat bran, 5-10 parts of bean dregs, 4-6 parts of fish meal, 2-3 parts of salt, 3-5 parts of traditional Chinese medicine additives and 6-8 parts of nutritional additives.

[0009] Furthermore, the preparation method of the traditional Chinese medicine additive comprises the following steps: mixing astragalus polysaccharide and glycoside compounds according to the mass ratio of astragalus polysaccharide to glycoside compounds of (2-4): (1-3) to obtain the traditional Chinese medicine additive.

[0010] Furthermore, the method for extracting astragalus polysaccharide comprises the following steps:

[0011] 1) According to the solid-liquid ratio of 1g:20mL, Astragalus was placed in a chloroform-methanol mixed solution and refluxed at 70°C for 3h to remove lipids and oligosaccharides in Astragalus;

[0012] 2) placing the astragalus after removing lipids and oligosaccharides in step 1) in water at a solid-liquid ratio of 1 g:30 mL and an ultrasonic power of 250 w, and extracting at 80° C. for 30 min to obtain an astragalus water extract;

[0013] 3) Concentrating the astragalus extract obtained in step 2) to 1 / 3 of the original volume to obtain an astragalus extract concentrate;

[0014] 4) adding the concentrated astragalus extract obtained in step 3) into 60% ethanol in a volume ratio of 1:1, precipitating at room temperature for 3 hours, filtering, washing and drying to obtain the astragalus polysaccharide.

[0015] Furthermore, in step 1), the chloroform-methanol mixed solution is prepared by mixing chloroform and methanol in a volume ratio of 2:1.

[0016] Furthermore, the method for extracting glycoside compounds comprises the following steps:

[0017] 1) mixing honeysuckle, forsythia, angelica dahurica and white peony root in a mass ratio of (2-4): (1-3): (0.5-1.5): (0.5-1.5) to obtain a mixed Chinese medicine;

[0018] 2) placing the mixed Chinese medicine obtained in step 1) in 60% ethanol, extracting at 60° C. for 2 h, repeating 3 times, combining the extracts, and then extracting with petroleum ether, ethyl acetate and n-butanol in sequence to obtain a petroleum ether portion, an ethyl acetate portion and an n-butanol portion;

[0019] 3) The ethyl acetate portion obtained in step 2) was applied to a silica gel column and gradient eluted using a methanol-water system as an eluent to obtain components F1a to F5a;

[0020] 4) The n-butanol portion obtained in step 2) was applied to a silica gel column and gradient eluted using a chloroform-methanol-water system as an eluent to obtain components F1b to F3b;

[0021] 5) The components F2a and F4a obtained in step 3) and the component F2b obtained in step 4) are mixed, distilled under reduced pressure, and dried to obtain the glycoside compound.

[0022] Furthermore, in step 3), the volume ratios of methanol and water in the methanol-water system are 2:8, 3:7, 5:5, 7:3, and 9:1, respectively.

[0023] Furthermore, in step 4), the volume ratios of chloroform, methanol and water in the chloroform-methanol-water system are 8:2:1, 7:3:1 and 5:5:1 respectively.

[0024] Furthermore, the preparation method of the nutritional additive comprises the following steps: glucose, calcium carbonate, lysine, threonine, vitamin A and vitamin E are mixed according to the mass ratio of glucose, calcium carbonate, lysine, threonine, vitamin A and vitamin E of (4-6): (2-4): (2-4): (0.5-1.5): (1-3): (0.5-1.5) to obtain the nutritional additive.

[0025] The second technical solution of the present invention:

[0026] The method for preparing the feed for preventing and treating porcine parvovirus disease comprises the following steps: mixing corn, wheat, wheat bran, bean dregs, fish meal, salt, Chinese medicine additives and nutritional additives, hot pressing and granulating to obtain the feed for preventing and treating porcine parvovirus disease.

[0027] Furthermore, the steam temperature of the hot pressing granulation is 90-100°C, and the discharge temperature is 45-55°C.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] The feed for preventing and treating porcine parvovirus disease provided by the invention has good prevention and treatment effect on porcine parvovirus disease. DETAILED DESCRIPTION

[0030] Now, various exemplary embodiments of the present invention are described in detail, and this detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics and embodiments of the present invention. It should be understood that the terms described in the present invention are only for describing specific embodiments and are not used to limit the present invention.

[0031] In addition, for the numerical range in the present invention, it is understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0032] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0033] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to the skilled artisan. The present invention description and examples are exemplary only.

[0034] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0035] In the following examples and comparative examples, the Chinese medicinal materials used were all purchased from commercial suppliers and ground to pass through a 40-mesh sieve.

[0036] In the following examples, a method for preparing a feed for preventing and treating porcine parvovirus disease comprises the following steps:

[0037] 1. Extraction of Astragalus Polysaccharide:

[0038] 1) Chloroform and methanol were mixed at a volume ratio of 2:1 to obtain a chloroform-methanol mixed solution, and then astragalus was placed in the chloroform-methanol mixed solution at a solid-liquid ratio of 1 g:20 mL, and refluxed at 70°C for 3 hours to remove lipids and oligosaccharides in the astragalus;

[0039] 2) placing the astragalus after removing lipids and oligosaccharides in step 1) in water at a solid-liquid ratio of 1 g:30 mL and an ultrasonic power of 250 w, and extracting at 80° C. for 30 min to obtain an astragalus water extract;

[0040] 3) Concentrating the astragalus extract obtained in step 2) to 1 / 3 of the original volume to obtain an astragalus extract concentrate;

[0041] 4) adding the concentrated astragalus extract obtained in step 3) to 60% ethanol in a volume ratio of 1:1, precipitating at room temperature for 3 hours, filtering, washing and drying to obtain astragalus polysaccharide;

[0042] 2. Extraction of glycoside compounds:

[0043] 1) mixing honeysuckle, forsythia, angelica dahurica and white peony root in a mass ratio of (2-4): (1-3): (0.5-1.5): (0.5-1.5) to obtain a mixed Chinese medicine;

[0044] 2) placing the mixed Chinese medicine obtained in step 1) in 60% ethanol, extracting at 60° C. for 2 h, repeating 3 times, combining the extracts, and then extracting with petroleum ether, ethyl acetate and n-butanol in sequence to obtain a petroleum ether portion, an ethyl acetate portion and an n-butanol portion;

[0045] 3) The ethyl acetate portion obtained in step 2) was applied to a silica gel column and gradient eluted using a methanol-water system as an eluent to obtain components F1a to F5a;

[0046] Wherein, the volume ratios of methanol to water in the methanol-water system are: 2:8, 3:7, 5:5, 7:3, 9:1;

[0047] 4) The n-butanol portion obtained in step 2) was applied to a silica gel column and gradient eluted using a chloroform-methanol-water system as an eluent to obtain components F1b to F3b;

[0048] The volume ratios of chloroform, methanol and water in the chloroform-methanol-water system are 8:2:1, 7:3:1 and 5:5:1 respectively.

[0049] 5) Mixing the components F2a and F4a obtained in step 3) and the component F2b obtained in step 4), distilling under reduced pressure, and drying to obtain a glycoside compound;

[0050] 3. Preparation of Chinese medicine additives:

[0051] According to the mass ratio of astragalus polysaccharide to glycoside compound being (2-4): (1-3), the astragalus polysaccharide obtained in step 1 and the glycoside compound obtained in step 2 are mixed to obtain a traditional Chinese medicine additive;

[0052] 4. Preparation of nutritional additives:

[0053] Glucose, calcium carbonate, lysine, threonine, vitamin A and vitamin E are mixed according to a mass ratio of glucose, calcium carbonate, lysine, threonine, vitamin A and vitamin E of (4-6): (2-4): (2-4): (0.5-1.5): (1-3): (0.5-1.5) to obtain a nutritional additive;

[0054] 5. Preparation of feed for the prevention and treatment of porcine parvovirus disease:

[0055] Corn, wheat, wheat bran, bean dregs, fish meal, salt, Chinese medicine additives and nutritional additives are mixed according to the mass ratio of corn, wheat, wheat bran, bean dregs, fish meal, salt, Chinese medicine additives and nutritional additives of (40-60): (15-25): (10-20): (5-10): (4-6): (2-3): (3-5): (6-8), and hot-pressed granulation is performed to obtain the feed for preventing and treating porcine parvovirus disease;

[0056] Wherein, the steam temperature of the hot pressing granulation is 90-100°C, and the discharge temperature is 45-55°C.

[0057] Example 1

[0058] Feed for preventing and treating porcine parvovirus disease

[0059] 1. Extraction of Astragalus Polysaccharide:

[0060] 1) Chloroform and methanol were mixed at a volume ratio of 2:1 to obtain a chloroform-methanol mixed solution, and then astragalus was placed in the chloroform-methanol mixed solution at a solid-liquid ratio of 1 g:20 mL, and refluxed at 70°C for 3 hours to remove lipids and oligosaccharides in the astragalus;

[0061] 2) placing the astragalus after removing lipids and oligosaccharides in step 1) in water at a solid-liquid ratio of 1 g:30 mL and an ultrasonic power of 250 w, and extracting at 80° C. for 30 min to obtain an astragalus water extract;

[0062] 3) Concentrating the astragalus extract obtained in step 2) to 1 / 3 of the original volume to obtain an astragalus extract concentrate;

[0063] 4) adding the concentrated astragalus extract obtained in step 3) to 60% ethanol in a volume ratio of 1:1, precipitating at room temperature for 3 hours, filtering, washing and drying to obtain astragalus polysaccharide;

[0064] 2. Extraction of glycoside compounds:

[0065] 1) mixing honeysuckle, forsythia, angelica dahurica and white peony root in a mass ratio of 2:1:0.5:0.5 to obtain a mixed traditional Chinese medicine;

[0066] 2) placing the mixed Chinese medicine obtained in step 1) in 60% ethanol, extracting at 60° C. for 2 h, repeating 3 times, combining the extracts, and then extracting with petroleum ether, ethyl acetate and n-butanol in sequence to obtain a petroleum ether portion, an ethyl acetate portion and an n-butanol portion;

[0067] 3) The ethyl acetate portion obtained in step 2) was applied to a silica gel column and gradient eluted using a methanol-water system as an eluent to obtain components F1a to F5a;

[0068] Wherein, the volume ratios of methanol to water in the methanol-water system are: 2:8, 3:7, 5:5, 7:3, 9:1;

[0069] 4) The n-butanol portion obtained in step 2) was applied to a silica gel column and gradient eluted using a chloroform-methanol-water system as an eluent to obtain components F1b to F3b;

[0070] The volume ratios of chloroform, methanol and water in the chloroform-methanol-water system are 8:2:1, 7:3:1 and 5:5:1 respectively.

[0071] 5) Mixing the components F2a and F4a obtained in step 3) and the component F2b obtained in step 4), distilling under reduced pressure, and drying to obtain a glycoside compound;

[0072] 3. Preparation of Chinese medicine additives:

[0073] According to the mass ratio of astragalus polysaccharide to glycoside compound of 2:1, the astragalus polysaccharide obtained in step 1 and the glycoside compound obtained in step 2 are mixed to obtain a traditional Chinese medicine additive;

[0074] 4. Preparation of nutritional additives:

[0075] Glucose, calcium carbonate, lysine, threonine, vitamin A and vitamin E are mixed according to a mass ratio of glucose, calcium carbonate, lysine, threonine, vitamin A and vitamin E of 4:2:2:0.5:1:0.5 to obtain a nutritional additive;

[0076] 5. Preparation of feed for the prevention and treatment of porcine parvovirus disease:

[0077] According to the mass ratio of corn, wheat, wheat bran, bean dregs, fish meal, salt, Chinese medicine additives and nutritional additives of 40:15:10:5:4:2:3:6, corn, wheat, wheat bran, bean dregs, fish meal, salt, Chinese medicine additives and nutritional additives are mixed, hot-pressed and granulated to obtain the feed for preventing and treating porcine parvovirus disease;

[0078] Wherein, the steam temperature of the hot pressing granulation is 90°C and the discharge temperature is 45°C.

[0079] Example 2

[0080] Feed for preventing and treating porcine parvovirus disease

[0081] 1. Extraction of Astragalus Polysaccharide:

[0082] 1) Chloroform and methanol were mixed at a volume ratio of 2:1 to obtain a chloroform-methanol mixed solution, and then astragalus was placed in the chloroform-methanol mixed solution at a solid-liquid ratio of 1 g:20 mL, and refluxed at 70°C for 3 hours to remove lipids and oligosaccharides in the astragalus;

[0083] 2) placing the astragalus after removing lipids and oligosaccharides in step 1) in water at a solid-liquid ratio of 1 g:30 mL and an ultrasonic power of 250 w, and extracting at 80° C. for 30 min to obtain an astragalus water extract;

[0084] 3) Concentrating the astragalus extract obtained in step 2) to 1 / 3 of the original volume to obtain an astragalus extract concentrate;

[0085] 4) adding the concentrated astragalus extract obtained in step 3) to 60% ethanol in a volume ratio of 1:1, precipitating at room temperature for 3 hours, filtering, washing and drying to obtain astragalus polysaccharide;

[0086] 2. Extraction of glycoside compounds:

[0087] 1) mixing honeysuckle, forsythia, angelica dahurica and white peony root in a mass ratio of 3:2:1:1 to obtain a mixed Chinese medicine;

[0088] 2) placing the mixed Chinese medicine obtained in step 1) in 60% ethanol, extracting at 60° C. for 2 h, repeating 3 times, combining the extracts, and then extracting with petroleum ether, ethyl acetate and n-butanol in sequence to obtain a petroleum ether portion, an ethyl acetate portion and an n-butanol portion;

[0089] 3) The ethyl acetate portion obtained in step 2) was applied to a silica gel column and gradient eluted using a methanol-water system as an eluent to obtain components F1a to F5a;

[0090] Wherein, the volume ratios of methanol to water in the methanol-water system are: 2:8, 3:7, 5:5, 7:3, 9:1;

[0091] 4) The n-butanol portion obtained in step 2) was applied to a silica gel column and gradient eluted using a chloroform-methanol-water system as an eluent to obtain components F1b to F3b;

[0092] The volume ratios of chloroform, methanol and water in the chloroform-methanol-water system are 8:2:1, 7:3:1 and 5:5:1 respectively.

[0093] 5) Mixing the components F2a and F4a obtained in step 3) and the component F2b obtained in step 4), distilling under reduced pressure, and drying to obtain a glycoside compound;

[0094] 3. Preparation of Chinese medicine additives:

[0095] According to the mass ratio of astragalus polysaccharide to glycoside compound of 3:2, the astragalus polysaccharide obtained in step 1 and the glycoside compound obtained in step 2 are mixed to obtain a traditional Chinese medicine additive;

[0096] 4. Preparation of nutritional additives:

[0097] Glucose, calcium carbonate, lysine, threonine, vitamin A and vitamin E are mixed according to a mass ratio of glucose, calcium carbonate, lysine, threonine, vitamin A and vitamin E of 5:3:3:1:2:1 to obtain a nutritional additive;

[0098] 5. Preparation of feed for the prevention and treatment of porcine parvovirus disease:

[0099] According to the mass ratio of corn, wheat, wheat bran, bean dregs, fish meal, salt, Chinese medicine additives and nutritional additives of 50:20:15:7:5:2.5:4:7, corn, wheat, wheat bran, bean dregs, fish meal, salt, Chinese medicine additives and nutritional additives are mixed, hot-pressed and granulated to obtain the feed for preventing and treating porcine parvovirus disease;

[0100] Wherein, the steam temperature of the hot pressing granulation is 95°C and the discharge temperature is 50°C.

[0101] Example 3

[0102] Feed for preventing and treating porcine parvovirus disease

[0103] 1. Extraction of Astragalus Polysaccharide:

[0104] 1) Chloroform and methanol were mixed at a volume ratio of 2:1 to obtain a chloroform-methanol mixed solution, and then astragalus was placed in the chloroform-methanol mixed solution at a solid-liquid ratio of 1 g:20 mL, and refluxed at 70°C for 3 hours to remove lipids and oligosaccharides in the astragalus;

[0105] 2) placing the astragalus after removing lipids and oligosaccharides in step 1) in water at a solid-liquid ratio of 1 g:30 mL and an ultrasonic power of 250 w, and extracting at 80° C. for 30 min to obtain an astragalus water extract;

[0106] 3) Concentrating the astragalus extract obtained in step 2) to 1 / 3 of the original volume to obtain an astragalus extract concentrate;

[0107] 4) adding the concentrated astragalus extract obtained in step 3) to 60% ethanol in a volume ratio of 1:1, precipitating at room temperature for 3 hours, filtering, washing and drying to obtain astragalus polysaccharide;

[0108] 2. Extraction of glycoside compounds:

[0109] 1) mixing honeysuckle, forsythia, angelica dahurica and white peony root in a mass ratio of 4:3:1.5:1.5 to obtain a mixed Chinese medicine;

[0110] 2) placing the mixed Chinese medicine obtained in step 1) in 60% ethanol, extracting at 60° C. for 2 h, repeating 3 times, combining the extracts, and then extracting with petroleum ether, ethyl acetate and n-butanol in sequence to obtain a petroleum ether portion, an ethyl acetate portion and an n-butanol portion;

[0111] 3) The ethyl acetate portion obtained in step 2) was applied to a silica gel column and gradient eluted using a methanol-water system as an eluent to obtain components F1a to F5a;

[0112] Wherein, the volume ratios of methanol to water in the methanol-water system are: 2:8, 3:7, 5:5, 7:3, 9:1;

[0113] 4) The n-butanol portion obtained in step 2) was applied to a silica gel column and gradient eluted using a chloroform-methanol-water system as an eluent to obtain components F1b to F3b;

[0114] The volume ratios of chloroform, methanol and water in the chloroform-methanol-water system are 8:2:1, 7:3:1 and 5:5:1 respectively.

[0115] 5) Mixing the components F2a and F4a obtained in step 3) and the component F2b obtained in step 4), distilling under reduced pressure, and drying to obtain a glycoside compound;

[0116] 3. Preparation of Chinese medicine additives:

[0117] According to the mass ratio of astragalus polysaccharide to glycoside compound of 4:3, the astragalus polysaccharide obtained in step 1 and the glycoside compound obtained in step 2 are mixed to obtain a traditional Chinese medicine additive;

[0118] 4. Preparation of nutritional additives:

[0119] Glucose, calcium carbonate, lysine, threonine, vitamin A and vitamin E are mixed according to a mass ratio of glucose, calcium carbonate, lysine, threonine, vitamin A and vitamin E of 6:4:4:1.5:3:1.5 to obtain a nutritional additive;

[0120] 5. Preparation of feed for the prevention and treatment of porcine parvovirus disease:

[0121] According to the mass ratio of corn, wheat, wheat bran, bean dregs, fish meal, salt, Chinese medicine additives and nutritional additives of 60:25:20:10:6:3:5:8, corn, wheat, wheat bran, bean dregs, fish meal, salt, Chinese medicine additives and nutritional additives are mixed, hot-pressed and granulated to obtain the feed for preventing and treating porcine parvovirus disease;

[0122] Wherein, the steam temperature of the hot pressing granulation is 100°C and the discharge temperature is 55°C.

[0123] Comparative Example 1

[0124] A kind of feed

[0125] The same as Example 2, except that the extraction of astragalus polysaccharide in step 1 is as follows:

[0126] 1) According to the solid-liquid ratio of 1g:30mL and the ultrasonic power of 250w, the astragalus was placed in water and extracted at 80℃ for 30min to obtain the astragalus water extract;

[0127] 2) concentrating the astragalus extract obtained in step 1) to 1 / 3 of the original volume to obtain an astragalus extract concentrate;

[0128] 3) According to the volume ratio of the astragalus extract concentrate to 60% ethanol being 1:1, the astragalus extract concentrate obtained in step 2) is added to 60% ethanol, and the mixture is precipitated at room temperature for 3 hours, filtered, washed, and dried to obtain astragalus polysaccharide.

[0129] Comparative Example 2

[0130] A kind of feed

[0131] The same as Example 2, except that the extraction of astragalus polysaccharide in step 1 is as follows:

[0132] 1) Chloroform and methanol were mixed at a volume ratio of 2:1 to obtain a chloroform-methanol mixed solution, and then astragalus was placed in the chloroform-methanol mixed solution at a solid-liquid ratio of 1 g:20 mL, and refluxed at 70°C for 3 hours to remove lipids and oligosaccharides in the astragalus;

[0133] 2) placing the astragalus after removing lipids and oligosaccharides in step 1) in water at a solid-liquid ratio of 1 g:30 mL, and extracting at 80° C. for 30 min to obtain an astragalus water extract;

[0134] 3) Concentrating the astragalus extract obtained in step 2) to 1 / 3 of the original volume to obtain an astragalus extract concentrate;

[0135] 4) According to the volume ratio of the astragalus extract concentrate to 60% ethanol being 1:1, the astragalus extract concentrate obtained in step 3) is added to 60% ethanol, and the mixture is precipitated at room temperature for 3 hours, filtered, washed, and dried to obtain astragalus polysaccharide.

[0136] Comparative Examples 3 to 10

[0137] A kind of feed

[0138] Same as Example 2, except that in step 2, in the extraction of glycoside compounds, step 1) is:

[0139] According to the mass ratio recorded in Table 1, honeysuckle, forsythia, angelica dahurica and white peony root are mixed to obtain a mixed Chinese medicine.

[0140] Table 1 Mass ratio

[0141]

[0142] Comparative Examples 11 to 18

[0143] A kind of feed

[0144] Same as Example 2, except that in the extraction of glycoside compounds in step 2, step 5) is:

[0145] According to the added components listed in Table 2, the components obtained in step 3) and the components obtained in step 4) were mixed, distilled under reduced pressure, and dried to obtain a glycoside compound.

[0146] Table 2 Added components

[0147]

[0148] Note: “√” indicates the addition of the component; different components may also contain other substances such as polysaccharides and alkaloids. To keep the wording consistent with the examples, glycoside compounds are used as the name of the final mixture.

[0149] Comparative Examples 19 to 24

[0150] A kind of feed

[0151] The same as Example 2, except that the preparation of the nutritional additive in step 4 is as follows:

[0152] Glucose, calcium carbonate, lysine, threonine, vitamin A and vitamin E were mixed according to the mass ratio shown in Table 3 to obtain a nutritional additive.

[0153] Table 3 Mass ratio

[0154]

[0155] Comparative Example 25

[0156] A kind of feed

[0157] Same as Example 2, except that step 5 is used in the preparation of feed for preventing and treating porcine parvovirus disease, the steam temperature of the hot pressing granulation is 110°C, and the discharge temperature is 50°C.

[0158] Comparative Example 26

[0159] A kind of feed

[0160] Same as Example 2, except that step 5 is used in the preparation of feed for preventing and treating porcine parvovirus disease, the steam temperature of the hot pressing granulation is 95°C, and the discharge temperature is 60°C.

[0161] Effect verification

[0162] 1. In vitro inhibitory effect of Chinese herbal medicine additives on porcine parvovirus

[0163] According to the concentration of the Chinese medicine additive of 0.1 g / mL, the Chinese medicine additives prepared in Examples 1 to 3 and Comparative Examples 1 to 18 were dissolved in sterile water for injection. Referring to "Study on the Inhibitory Effect of Astragalus and Radix Isatidis on Porcine Parvovirus in Vitro", Wang Xuebin et al., the Chinese medicine additives prepared in Examples 1 to 3 and Comparative Examples 1 to 18 were tested for the in vitro inhibition of porcine parvovirus. The results of the in vitro inhibition test of porcine parvovirus are shown in Table 4.

[0164] Table 4 Results of in vitro inhibition test of porcine parvovirus

[0165]

[0166] It can be seen from the data in Table 4 that the Chinese medicinal additives prepared in Examples 2 to 3 can directly kill porcine parvovirus, while the Chinese medicinal additives prepared in Example 1 and Comparative Examples 1 to 18 cannot directly kill porcine parvovirus.

[0167] In order to further verify the preventive and therapeutic effect of the feed prepared by the present invention on porcine parvovirus disease, the feeds prepared by Examples 1 to 3 and Comparative Examples 2, 8, 10 to 11, 13 to 15, and 19 to 26 were tested for the preventive and therapeutic effect of porcine parvovirus disease;

[0168] The specific test method is: one-year-old sows infected with porcine parvovirus are used as experimental animals (5 in each group, and the test results are calculated as the average value), and the test feed is fed twice a day. Then, the blood of the experimental animals is tested for porcine parvovirus disease by PCR on the 0th day, the 7th day and the 14th day of feeding. The porcine parvovirus disease PCR test results are shown in Table 5;

[0169] Blank control group: The feed included the following raw materials by weight: 50 parts of corn, 20 parts of wheat, 15 parts of wheat bran, 7 parts of bean dregs, 5 parts of fish meal and 2.5 parts of salt;

[0170] Table 5 PCR test results of porcine parvovirus disease

[0171]

[0172] It can be seen from the data in Table 5 that the feed for preventing and treating porcine parvovirus disease provided by the present invention has a good prevention and treatment effect on porcine parvovirus disease.

[0173] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the relevant field should understand that the specific implementation methods of the present invention can still be modified or replaced by equivalents. Any modification or equivalent replacement that does not depart from the spirit and scope of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A feed for preventing and treating porcine parvovirus disease, characterized in that: The invention comprises the following raw materials in parts by weight: 40-60 parts of corn, 15-25 parts of wheat, 10-20 parts of wheat bran, 5-10 parts of bean dregs, 4-6 parts of fish meal, 2-3 parts of salt, 3-5 parts of traditional Chinese medicine additives and 6-8 parts of nutritional additives.

2. A feed for preventing and treating porcine parvovirus disease according to claim 1, characterized in that: The preparation method of the traditional Chinese medicine additive comprises the following steps: mixing astragalus polysaccharide and glycoside compounds according to the mass ratio of astragalus polysaccharide to glycoside compounds being (2-4): (1-3) to obtain the traditional Chinese medicine additive.

3. A feed for preventing and treating porcine parvovirus disease according to claim 2, characterized in that: The method for extracting astragalus polysaccharide comprises the following steps: 1) According to the solid-liquid ratio of 1g:20mL, Astragalus was placed in a chloroform-methanol mixed solution and refluxed at 70°C for 3h to remove lipids and oligosaccharides in Astragalus; 2) placing the astragalus after removing lipids and oligosaccharides in step 1) in water at a solid-liquid ratio of 1 g:30 mL and an ultrasonic power of 250 w, and extracting at 80° C. for 30 min to obtain an astragalus water extract; 3) concentrating the astragalus extract obtained in step 2) to 1 / 3 of the original volume to obtain an astragalus extract concentrate; 4) adding the concentrated astragalus extract obtained in step 3) into 60% ethanol in a volume ratio of 1:1, precipitating at room temperature for 3 hours, filtering, washing and drying to obtain the astragalus polysaccharide.

4. A feed for preventing and treating porcine parvovirus disease according to claim 3, characterized in that: In step 1), the chloroform-methanol mixed solution is prepared by mixing chloroform and methanol in a volume ratio of 2:

1.

5. The feed for preventing and treating porcine parvovirus disease according to claim 2, characterized in that: The method for extracting glycoside compounds comprises the following steps: 1) mixing honeysuckle, forsythia, angelica dahurica and white peony root in a mass ratio of (2-4): (1-3): (0.5-1.5): (0.5-1.5) to obtain a mixed Chinese medicine; 2) placing the mixed Chinese medicine obtained in step 1) in 60% ethanol, extracting at 60° C. for 2 h, repeating 3 times, combining the extracts, and then extracting with petroleum ether, ethyl acetate and n-butanol in sequence to obtain a petroleum ether portion, an ethyl acetate portion and an n-butanol portion; 3) The ethyl acetate portion obtained in step 2) was applied to a silica gel column and gradient eluted using a methanol-water system as an eluent to obtain components F1a to F5a; 4) The n-butanol portion obtained in step 2) was applied to a silica gel column and gradient eluted using a chloroform-methanol-water system as an eluent to obtain components F1b to F3b; 5) The components F2a and F4a obtained in step 3) and the component F2b obtained in step 4) are mixed, distilled under reduced pressure, and dried to obtain the glycoside compound.

6. A feed for preventing and treating porcine parvovirus disease according to claim 5, characterized in that: In step 3), the volume ratios of methanol to water in the methanol-water system are 2:8, 3:7, 5:5, 7:3, and 9:1, respectively.

7. The feed for preventing and treating porcine parvovirus disease according to claim 5, characterized in that: In step 4), the volume ratios of chloroform, methanol and water in the chloroform-methanol-water system are 8:2:1, 7:3:1 and 5:5:1, respectively.

8. The feed for preventing and treating porcine parvovirus disease according to claim 1, characterized in that: The preparation method of the nutritional additive comprises the following steps: glucose, calcium carbonate, lysine, threonine, vitamin A and vitamin E are mixed according to the mass ratio of glucose, calcium carbonate, lysine, threonine, vitamin A and vitamin E being (4-6): (2-4): (2-4): (0.5-1.5): (1-3): (0.5-1.5) to obtain the nutritional additive.

9. A method for preparing a feed for preventing and treating porcine parvovirus disease according to any one of claims 1 to 8, characterized in that: The following steps are involved: Corn, wheat, wheat bran, bean dregs, fish meal, salt, traditional Chinese medicine additives and nutritional additives are mixed, hot-pressed and granulated to obtain the feed for preventing and treating porcine parvovirus disease.

10. The preparation method according to claim 9, characterized in that: The steam temperature of the hot pressing granulation is 90-100°C, and the discharge temperature is 45-55°C.

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