Functional food for enhancing immunity and regulating blood fat and two-step fermentation preparation method thereof

Abstract

The invention discloses a functional food for enhancing immunity and regulating blood fat and a two-step fermentation preparation method thereof.The two-step fermentation preparation method comprises the steps that firstly, cordyceps militaris mycelia and rhizoma polygonati are subjected to two-way solid fermentation, nutrition is provided for the cordyceps militaris mycelia through the rhizoma polygonati, meanwhile, by means of the enzymolysis effect of cordyceps militaris, the medicine effect of the rhizoma polygonati is changed, and the effect of the rhizoma polygonati is changed; preparing a medicinal mycoplasm-cordyceps militaris-rhizoma polygonati solid fermentation product with the functions of enhancing immunity and regulating blood fat; secondly, the obtained cordyceps militaris-rhizoma polygonati solid fermentation product is subjected to lactobacillus fermentation, so that the content of cordycepin, cordyceps polysaccharide and rhizoma polygonati polysaccharide is further increased; in addition, simple raw materials such as prebiotics, sugar alcohol and the like are matched, so that a composition product formula of a medicine or food with the functions of enhancing immunity and regulating blood fat is obtained.

Description

Technical Field

[0001] The invention belongs to the technical field, and specifically relates to a functional food for enhancing immunity and regulating blood lipids and a two-step fermentation preparation method thereof. Background Art

[0002] Cordyceps militaris, also known as northern cordyceps, belongs to the genus Cordyceps of the family Clavicipitaceae. It is of the same genus and species as Cordyceps sinensis, and its health-care effects are similar. Cordyceps militaris contains a variety of active substances such as cordycepic acid, cordycepin, cordyceps polysaccharides, and cordyceps peptides, which have good pharmacological effects in immunomodulation, anti-tumor, and hypoglycemic effects. At the same time, natural active ingredients such as cordyceps peptides, cordycepin, and cordyceps polysaccharides have biological activities for lowering blood lipids, and are also well used in improving the symptoms of hyperlipidemia. Cordyceps militaris polysaccharides are one of the main active ingredients in Cordyceps militaris, and have multiple biological activities such as α-glucosidase inhibition, anti-inflammatory, antioxidant, and antibacterial. A large number of studies have shown that the nutritional and medicinal components of Cordyceps militaris are similar to those of Cordyceps sinensis, and the content of some active ingredients such as adenosine and cordycepin is higher. Because Cordyceps militaris has a high tonic effect and significant medicinal value, it has gradually become a substitute for Cordyceps sinensis and has been used in the food, medical and health care products industries, with growing demand.

[0003] Polygonatum sibiricum is a perennial herbaceous plant of the genus Polygonatum in the monocotyledonous Liliaceae family. It is the dried rhizome of Polygonatum multiflorum, Polygonatum sibiricum, and Polygonatum yunnanensis. It has the effects of replenishing qi and nourishing yin, strengthening the spleen, moistening the lungs, and benefiting the kidneys. It can be used for spleen and stomach qi deficiency, insufficient stomach yin, lung deficiency and dry cough, insufficient essence and blood, soreness of waist and knees, premature graying of hair, internal heat and thirst, etc. It enjoys the reputation of "the king of blood and qi supplement". It has been widely used as a medicinal and edible product for many years, and has the activities of immunomodulation, lowering blood sugar, lowering blood lipids, anti-inflammatory, antioxidant, anti-tumor and anti-osteoporosis.

[0004] Bidirectional solid fermentation is the process of constructing a fermentation combination of medicinal fungi and Chinese medicinal materials. While the medicinal materials provide nutrition for the fungi, the original chemical components are transformed due to the action of fungal enzymes, resulting in different functions, properties and tastes from the original ones, so it has "bidirectionality". Liquid deep fermentation refers to adding culture medium and passing sterile air into a specific biochemical reactor, stirring or oscillating it and controlling suitable external conditions, so that the fungus can reproduce and develop in the liquid, obtain a large amount of mycelium and metabolites, and obtain physiologically active substances that fruiting bodies cannot produce or have a higher content than fruiting bodies. Bidirectional solid fermentation technology has the functions of enhancing drug efficacy, reducing toxicity, detoxification, expanding drug varieties, and improving the utilization rate of important resources. With the continuous development of contemporary Chinese medicine, the fermentation of Chinese herbal medicines has gradually become a research hotspot in the field of medicine. Introducing probiotics in the process of Chinese herbal medicine fermentation can further promote the transformation and release of Chinese herbal medicine components, improve drug efficacy and produce beneficial compounds.

[0005] Literature has shown that Lactobacillus plantarum CCFM8661 has the effects of enhancing immunity, removing lead, alleviating liver and kidney damage induced by heavy metals, and regulating intestinal flora. Literature has shown that Lactobacillus paracasei Glory LP16 and Lactobacillus rhamnosus Glory LG12 have the effects of regulating intestinal dysfunction.

[0006] Chinese patent ZL202211722631.5 discloses the application of a bidirectional solid fermentation product of Tripterygium wilfordii in drugs for treating kidney disease, and confirms that the obtained bidirectional solid fermentation product of Tripterygium wilfordii has low toxic side effects and better therapeutic effects in treating chronic kidney disease. However, there is no document that discloses a two-step fermentation process of first fermenting Polygonatum sibiricum with Cordyceps militaris as the fermentation strain, and then fermenting with lactic acid bacteria, and its application in enhancing immunity and regulating blood lipids. Summary of the invention

[0007] Purpose of the invention: In order to overcome the above technical defects, the present invention proposes a functional food for enhancing immunity and regulating blood lipids and a two-step fermentation preparation method thereof, wherein Cordyceps militaris mycelium and Polygonatum sibiricum are subjected to bidirectional solid fermentation, Polygonatum sibiricum provides nutrition for Cordyceps militaris mycelium, and at the same time, the enzymatic hydrolysis of Cordyceps militaris promotes the release of the medicinal effect of Polygonatum sibiricum, thereby preparing a medicinal fungus with the function of enhancing immunity and regulating blood lipids, and further combining it with lactic acid bacteria fermentation to achieve two-step fermentation, thereby further increasing the content of cordycepin, Cordyceps polysaccharide, and Polygonatum sibiricum polysaccharide.

[0008] Technical solution: To achieve the above purpose, the technical solution adopted by the present invention is:

[0009] The first aspect of the present invention provides a two-step fermentation method for preparing a functional food for enhancing immunity and regulating blood lipids, comprising the following steps:

[0010] S1, the activated Cordyceps militaris is fermented and cultured in a fermentation medium with Polygonatum sibiricum as the only matrix source, and the Cordyceps militaris mycelium and Polygonatum sibiricum are subjected to a two-way solid fermentation to obtain a Cordyceps militaris-Polygonatum sibiricum solid fermentation product;

[0011] S2. The obtained Cordyceps militaris-Polygonatum solid fermentation product is subjected to lactic acid bacteria fermentation to obtain Cordyceps militaris-Polygonatum-probiotic fermentation product.

[0012] Optionally, in some embodiments of the present invention, in step S1, the fermentation medium consists of Polygonatum sibiricum and 20-40% volume of water.

[0013] Preferably, the particle size of the polygonatum is 50-100 mesh.

[0014] Preferably, the method for preparing the fermentation medium comprises: crushing polygonatum to 50-100 mesh, mixing evenly with pure water, wherein the volume ratio of pure water to polygonatum powder is 0.2-0.4:1, and sterilizing to obtain the fermentation medium.

[0015] Optionally, in some embodiments of the present invention, in step S1, in the bidirectional solid fermentation, the inoculation amount of Cordyceps militaris is 2-5%.

[0016] Optionally, in some embodiments of the present invention, in step S1, the bidirectional solid fermentation includes culturing at 24-28° C. and 30-50% relative humidity in the dark.

[0017] Optionally, in some embodiments of the present invention, in step S2, the Cordyceps militaris-Polygonatum solid fermentation product is dried and crushed before being fermented by lactic acid bacteria.

[0018] Preferably, the Cordyceps militaris-Polygonatum solid fermentation product is crushed to 50-100 mesh.

[0019] Preferably, the drying comprises drying at 50°C-65°C to constant weight.

[0020] Optionally, in some embodiments of the present invention, in step S2, in the lactic acid bacteria fermentation system, the mass concentration of the Cordyceps militaris-Polygonatum solid fermentation product is 10-30%, and the inoculation amount of lactic acid bacteria is 2%-5%.

[0021] Optionally, in some embodiments of the present invention, in step S2, in the lactic acid bacteria fermentation system, the lactic acid bacteria include one or more of Lactobacillus rhamnosus Glory LG12, Lactobacillus paracasei Glory LP16, and Lactobacillus plantarum CCFM8661.

[0022] Preferably, Lactobacillus plantarum CCFM8661; and / or

[0023] Optionally, in some embodiments of the present invention, in step S2, the conditions for lactic acid bacteria fermentation include culturing at 37° C. for 3-5 days and stopping the fermentation when the pH reaches 4.4.

[0024] Optionally, in some embodiments of the present invention, the Cordyceps militaris-Polygonatum-probiotic fermentation product obtained in step S2 is further filtered and freeze-dried to obtain Cordyceps militaris-Polygonatum-probiotic freeze-dried powder.

[0025] The second aspect of the present invention provides a Cordyceps militaris-Polygonatum-probiotic fermentation product prepared by any of the above methods.

[0026] Optionally, in some embodiments of the present invention, the Cordyceps militaris-Polygonatum-probiotic fermentation product has the ability to enhance immunity and regulate blood lipids.

[0027] Optionally, in some embodiments of the present invention, the Cordyceps militaris-Polygonatum-probiotic fermentation product contains more than 8.0 mg / g of cordycepin and more than 30.0 mg / g of Cordyceps militaris-Polygonatum polysaccharide.

[0028] Preferably, the Cordyceps militaris-Polygonatum-probiotic fermentation product contains more than 10.0 mg / g of cordycepin and more than 31.0 mg / g of Cordyceps militaris-Polygonatum polysaccharide.

[0029] Further preferably, the Cordyceps militaris-Polygonatum-probiotic fermentation product contains more than 13.0 mg / g of cordycepin and more than 35.0 mg / g of Cordyceps militaris-Polygonatum polysaccharide.

[0030] The third aspect of the present invention provides a composition comprising the Cordyceps militaris-Polygonatum sibiricum-probiotic fermentation product as described in any one of the above.

[0031] Optionally, in some embodiments of the present invention, the composition further comprises a pharmaceutically acceptable carrier or excipient;

[0032] Optionally, in some embodiments of the present invention, the composition is in the form of granules, tablets or capsules.

[0033] The fourth aspect of the present invention provides the use of any of the above Cordyceps militaris-Polygonatum-probiotic fermentation products, or the above composition in food or medicine.

[0034] Beneficial effects: The present invention provides a functional food for enhancing immunity and regulating blood lipids and a two-step fermentation preparation method thereof. First, Cordyceps militaris mycelium and Polygonatum sibiricum are subjected to bidirectional solid fermentation, Polygonatum sibiricum provides nutrition for Cordyceps militaris mycelium, and at the same time, with the help of the enzymatic hydrolysis of Cordyceps militaris, the medicinal effect of Polygonatum sibiricum is changed, so that a medicinal fungus with the function of enhancing immunity and regulating blood lipids, namely, Cordyceps militaris-Polygonatum sibiricum solid fermentation product, is prepared; secondly, the obtained Cordyceps militaris-Polygonatum sibiricum solid fermentation product is subjected to lactic acid bacteria fermentation, so as to further expand the content of cordycepin, Cordyceps polysaccharide and Polygonatum sibiricum polysaccharide; in addition, by combining simple raw materials such as prebiotics and sugar alcohols, a composition product formula of a medicine or food with the function of enhancing immunity and regulating blood lipids is obtained. DETAILED DESCRIPTION

[0035] The first technical problem to be solved by the present invention is to provide a Cordyceps militaris-Polygonatum bidirectional solid fermentation product formed by co-fermentation of Cordyceps militaris and Polygonatum, which effectively expands the medicinal methods of Cordyceps militaris and Polygonatum, further improves the medicinal effects of Cordyceps militaris and Polygonatum, and prepares a Cordyceps militaris-Polygonatum fermentation culture with the effects of enhancing immunity and regulating blood lipids.

[0036] The second technical problem to be solved by the present invention is to provide a method for preparing the two-step fermentation product of the above-mentioned Cordyceps militaris-Polygonatum sibiricum fermentation culture, thereby increasing the content of cordycepin, Cordyceps polysaccharide, and Polygonatum sibiricum polysaccharide, and preparing Cordyceps militaris-Polygonatum sibiricum-probiotic freeze-dried powder.

[0037] The third technical problem to be solved by the present invention is to provide the application of the above-mentioned two-step fermentation product Cordyceps militaris-Polygonatum-probiotic freeze-dried powder for enhancing immunity and regulating blood lipids.

[0038] Based on this, the present invention provides a two-step fermentation preparation method for functional food for enhancing immunity and regulating blood lipids, comprising the following steps:

[0039] (1) Cordyceps militaris-Polygonatum bidirectional solid fermentation: Polygonatum sibiricum is used as the medicinal culture matrix and inoculated with Cordyceps militaris liquid strain for bidirectional solid fermentation to prepare medicinal fungus with the effects of enhancing immunity and regulating blood lipids.

[0040] (2) Liquid fermentation with lactic acid bacteria: The medicinal fungus obtained in step (1) is used as raw material, dried and crushed, and then inoculated with lactic acid bacteria for liquid fermentation to increase the content of cordycepin, cordyceps polysaccharide, and polygonatum polysaccharide in the culture of Cordyceps militaris and Polygonatum cyrtonema.

[0041] (3) The fermentation liquid of step (2) is filtered, centrifuged and freeze-dried to prepare Cordyceps militaris-Polygonatum-probiotic freeze-dried powder.

[0042] (4) Using the Cordyceps militaris-Polygonatum sibiricum probiotic freeze-dried powder of step (3) as a raw material, a functional product in the form of granules, tablets, and capsules is prepared.

[0043] The following is a detailed and specific description of the two-step fermentation preparation method of a functional food for enhancing immunity and regulating blood lipids of the present invention.

[0044] Example

[0045] A two-step fermentation preparation method for a functional food for enhancing immunity and regulating blood lipids comprises the following steps.

[0046] S1. Cordyceps militaris-Polygonatum sibiricum bidirectional solid fermentation preparation method

[0047] (1) Activation of Cordyceps militaris

[0048] The Cordyceps militaris strain CICC14013 was inoculated into the PDA slant medium and cultured at 28°C for 7 days. When the mycelium covered the slant, it was set aside.

[0049] Slant medium: 20% potato, 2% glucose, 0.1% KH2PO4, 0.1% MgSO4, 2.5% agar.

[0050] (2) Cordyceps militaris seed liquid culture

[0051] The mycelium is picked from the Cordyceps militaris slant culture medium and inoculated into the primary seed solution, and cultured at 24-28°C and 160-200r / min shaking conditions for 3-5 days. After the primary culture solution is inoculated with 5% of the secondary seed solution, it is placed in a constant temperature shaker and cultured for 3-5 days to prepare the Cordyceps militaris seed solution.

[0052] Shake flask seed culture medium: 2% glucose, 1% peptone, 0.05% KH2PO4, 0.05% MgSO4.

[0053] (3) Cordyceps militaris-Polygonatum bidirectional solid fermentation culture

[0054] Weigh Polygonatum sibiricum, crush it into 50-100 mesh size, mix it evenly with 20-40% volume of distilled water, sterilize it once at 121° C. for 30 min, shake it loose and set it aside.

[0055] The mycelium in the secondary seed liquid of Cordyceps militaris is collected on a sterile filter membrane by a vacuum filtration method, and then mixed with a polygonatum sibiricum matrix at a ratio of 2-5%. The culture is placed in the dark under the conditions of 24-28° C. and a relative humidity of 30-50%, and when the mycelium of Cordyceps militaris covers the entire medicinal matrix and forms an obvious fungus bud structure, the fermentation is terminated to obtain a Cordyceps militaris-polygonatum sibiricum fermentation culture.

[0056] The Cordyceps militaris-Polygonatum fermented culture is placed in a hot air circulation drying oven and dried at 50°C-65°C to constant weight. After drying, it is crushed into 50-100 meshes, packaged and placed in a desiccator for storage.

[0057] S2. Lactic acid bacteria liquid fermentation of Cordyceps militaris and Polygonatum sibiricum

[0058] Add the Cordyceps militaris-Polygonatum bidirectional solid fermentation culture powder into the fermentation tank, add pure water to make a concentration of 10-30%, and then add 2%-5% of 10 7 CFU / mL lactic acid bacteria (including but not limited to Lactobacillus rhamnosus Glory LG12, Lactobacillus paracasei Glory LP16 or Lactobacillus plantarum CCFM8661) seed liquid, mixed evenly, then cultured at 37° C. for 3-5 days, and stopped fermentation when the pH reached 4.4.

[0059] S3. Preparation of Cordyceps militaris-Polygonatum sibiricum two-step fermentation product

[0060] (1) Filtration: After the Cordyceps militaris-Polygonatum lactic acid bacteria fermentation is completed, use gauze to filter and remove the filter residue.

[0061] (2) Centrifugation: Centrifuge at 4000 r / min for 10 min to obtain the fermentation supernatant.

[0062] (3) Freeze drying: -30 to -50°C, 0.05-0.1 MPa, vacuum freeze drying for 10-48 hours to obtain Cordyceps militaris-Polygonatum sibiricum probiotic freeze-dried powder.

[0063] S4. Product preparation

[0064] (1) Preparation of Cordyceps militaris-Polygonatum-probiotic granule product:

[0065] The formula is mainly composed of the following materials in parts by weight:

[0066] Cordyceps militaris-Polygonatum-probiotic freeze-dried powder: 20-40 portions,

[0067] Sugar alcohols (including but not limited to sorbitol, erythritol, xylitol, isomalt, D-mannitol): 20-40 parts,

[0068] Prebiotics (including but not limited to fructooligosaccharides, galacto-oligosaccharides, xylo-oligosaccharides, stachyose, resistant dextrin): 20-40 parts,

[0069] Acidity regulator (including but not limited to citric acid, DL-malic acid, tartaric acid): 0.5-5 parts,

[0070] Sweetness regulators (including but not limited to mogroside, steviol glycosides, sucralose, aspartame, acesulfame potassium): 0.5-2 parts,

[0071] Binder (including but not limited to sodium carboxymethyl cellulose, gum arabic): 0.1-1 part.

[0072] The production process of Cordyceps militaris and Polygonatum sibiricum probiotic granules is as follows:

[0073] (1.1) Ingredients: Accurately weigh each material according to the product formula, and prepare the adhesive into a 2-5% adhesive solution using pure water.

[0074] (1.2) Mixing: Put the weighed materials except Cordyceps militaris-Polygonatum sibiricum-probiotic freeze-dried powder into a mixing tank in sequence, and mix at 600 r / min for 30-60 min until the mixture is uniform.

[0075] (1.3) Granulation: The mixed materials are put into a wet granulator, 1-5% binder solution is added, and the granulation is completed after high-speed shearing and stirring.

[0076] (1.4) Drying: Boiling drying, drying until the moisture content is less than 5%.

[0077] (1.5) Granulation: The dried material is granulated through a 10-20 mesh. The material after granulation is granular.

[0078] (1.6) Total mixing: The granular material is mixed with the Cordyceps militaris-Polygonatum sibiricum-probiotic freeze-dried powder in a mixing tank at 600 r / min for 30-60 min until the mixture is uniform, thereby obtaining the Cordyceps militaris-Polygonatum sibiricum-probiotic granules.

[0079] (1.7) Packaging: Fill the Cordyceps militaris-Polygonatum sibiricum-probiotic granules into aluminum foil bags, 2 g / bag.

[0080] (1.8) Metal inspection: The product is passed through a metal detector, process parameters: iron Fe<Ф1.2mm, stainless steel SUS<Ф1.2mm.

[0081] (2) Preparation of Cordyceps militaris-Polygonatum-probiotic tablet product:

[0082] The formula is mainly composed of the following materials in parts by weight:

[0083] Cordyceps militaris-Polygonatum-probiotic freeze-dried powder: 20-40 portions,

[0084] Sugar alcohols (including but not limited to sorbitol, erythritol, xylitol, isomalt, D-mannitol): 20-40 parts,

[0085] Prebiotics (including but not limited to fructooligosaccharides, galacto-oligosaccharides, xylo-oligosaccharides, stachyose, resistant dextrin): 20-40 parts,

[0086] Acidity regulator (including but not limited to citric acid, DL-malic acid, tartaric acid): 0.5-5 parts,

[0087] Sweetness regulators (including but not limited to mogroside, steviol glycosides, sucralose, aspartame, acesulfame potassium): 0.5-2 parts,

[0088] Binder (including but not limited to sodium carboxymethyl cellulose, gum arabic): 0.1-1 part,

[0089] Anticaking agents (including but not limited to magnesium stearate, silicon dioxide): 0.5-2 parts.

[0090] The production process of Cordyceps militaris-Polygonatum-probiotic tablets is as follows:

[0091] (2.1) Ingredients: Accurately weigh each material according to the product formula, and prepare the adhesive into a 2-5% adhesive solution using pure water.

[0092] (2.2) Mixing: Put the weighed materials except Cordyceps militaris-Polygonatum sibiricum probiotic freeze-dried powder into a mixing tank in sequence, and mix at 600 r / min for 30-60 min until the mixture is uniform.

[0093] (2.3) Granulation: The mixed materials are put into a wet granulator, 1-5% binder solution is added, and the granulation is completed after high-speed shearing and stirring.

[0094] (2.4) Drying: Boiling drying, drying until the moisture content is less than 5%.

[0095] (2.5) Granulation: The dried material is granulated through a 10-20 mesh. The material after granulation is granular material.

[0096] (2.6) Total mixing: The granular material is mixed with the Cordyceps militaris-Polygonatum sibiricum probiotic freeze-dried powder in a mixing tank at 600 r / min for 30-60 min until the mixture is uniform, thereby obtaining the Cordyceps militaris-Polygonatum sibiricum-probiotic granules.

[0097] (2.7) Tablet pressing: put the Cordyceps militaris-Polygonatum sibiricum-probiotic granules into a tablet press and compress them into tablets at 1 g / tablet.

[0098] (2.8) Packaging: Bottle the Cordyceps militaris-Polygonatum-probiotic tablets, 60 tablets / bottle.

[0099] (2.9) Metal inspection: The product is passed through a metal detector, process parameters: iron Fe<Ф1.2mm, stainless steel SUS<Ф1.2mm.

[0100] (3) Preparation of Cordyceps militaris-Polygonatum-probiotic capsule dosage form product:

[0101] The formula is mainly composed of the following substances in parts by weight:

[0102] Cordyceps militaris-Polygonatum-probiotic freeze-dried powder: 40-80 portions,

[0103] Prebiotics (including but not limited to fructooligosaccharides, galacto-oligosaccharides, xylo-oligosaccharides, stachyose, resistant dextrin): 20-40 parts,

[0104] Anticaking agents (including but not limited to magnesium stearate, silicon dioxide): 0.5-2 parts.

[0105] The production process of Cordyceps militaris-Polygonatum-probiotic capsules is as follows:

[0106] (3.1) Ingredients: Accurately weigh each material according to the product formula.

[0107] (3.2) Mixing: Put the weighed materials into the mixing tank in turn, mix at 600r / min for 30-60min until they are evenly mixed.

[0108] (3.3) Canning: Use 0# capsule shell, put the mixed material into the capsule machine, and can 0.5g / capsule.

[0109] (3.4) Packaging: Bottle the Cordyceps militaris and Polygonatum sibiricum probiotic capsules, 60 capsules / bottle.

[0110] (3.5) Metal inspection: The product is passed through a metal detector, process parameters: iron Fe<Ф1.2mm, stainless steel SUS<Ф1.2mm.

[0111] The protection points of this patent include at least the following two points:

[0112] (1) Innovation of the two-step fermentation process of Cordyceps militaris-Polygonatum: First, Cordyceps militaris mycelium and Polygonatum sibiricum are used for bidirectional solid fermentation to obtain medicinal fungi with the effects of enhancing immunity and regulating blood lipids; second, the medicinal fungi are deep fermented with lactic acid bacteria to further increase the content of cordycepin, cordyceps polysaccharides, and Polygonatum sibiricum polysaccharides in the medicinal fungi, and cooperate with lactic acid bacteria to obtain Cordyceps militaris-Polygonatum sibiricum-probiotic freeze-dried powder with the effects of enhancing immunity and regulating blood lipids.

[0113] (2) Product formula innovation with the effects of enhancing immunity and regulating blood lipids: Use Cordyceps militaris-Polygonatum-probiotic freeze-dried powder, combined with simple raw materials such as prebiotics and sugar alcohols, to prepare granule products, tablet products, and capsule products with the functions of enhancing immunity and regulating blood lipids.

[0114] The present invention will be further described below in conjunction with examples. According to the following examples, the present invention can be better understood. However, it is easy for those skilled in the art to understand that the specific material ratios, process conditions and results described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

[0115] The method for determining the efficacy content in the examples of the present invention is as follows.

[0116] (1) Determination of cordycepin

[0117] The sample was prepared into a 10% solution, filtered through a 0.22 μm filter membrane and placed in a sample injection bottle for HPLC determination. The detection conditions were: chromatographic column C18; mobile phase was 85% by volume ddH2O and 15% by volume methanol; flow rate was 1 mL / min;

[0118] (2) Determination of polysaccharides

[0119] Accurately weigh 25.0 mg of glucose, add purified water to dissolve, and dilute to a 25 mL volumetric flask to obtain a 1.0 mg / mL glucose mother solution. Accurately measure 1.0, 2.0, 3.0, 4.0, and 5.0 mL of the mother solution in a 100 mL volumetric flask, dilute to volume, and respectively draw 1.0 mL of each concentration of glucose standard solution into a stoppered test tube, add 0.05 mL of 80% phenol and 5.0 mL of concentrated sulfuric acid, shake well, let stand for 5 minutes, heat in a boiling water bath for 10 minutes, take out, cool to room temperature, and measure the absorbance at a wavelength of 490 nm. Draw a standard curve with glucose concentration (μg / mL) as the X-axis and absorbance as the Y-axis. Take the sample to be tested and configure it into a 10% concentration solution, and measure the absorbance at a wavelength of 490 nm. Read the polysaccharide content according to the standard curve.

[0120] (3) Determination of lactic acid bacteria count

[0121] The determination is carried out in accordance with GB 4789.35-2023 "National Food Safety Standard Food Microbiology Examination Lactic Acid Bacteria Examination".

[0122] (4) Mouse experiments

[0123] (4.1) Experimental animals: 20 male mice aged 6-8 weeks, weighing 18±2 g.

[0124] (4.2) Experimental reagents: serum cholesterol, triglycerides, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, immunoglobulin G, immunoglobulin M, cytokine IL-2, and cytokine IL-6 ELISA kits.

[0125] (4.3) Experimental method: After the mice were fed with a high-fat diet for 28 days, they were divided into two groups, with 10 mice in each group. Experimental group 1 was the control group, which was fed normally and orally gavaged with 0.9% saline once a day; experimental group 2 was the Cordyceps militaris and Polygonatum solid fermentation culture group, which was fed normally and orally gavaged with 20mL / kg of the 50% concentration of the test solution once a day. The light cycle was 12h, and the mice were fed for 28 days. After anesthesia, the eyeballs were removed, and the serum was centrifuged and tested according to the operating instructions of the kit.

[0126] Example 1 Cordyceps militaris-Polygonatum bidirectional solid fermentation

[0127] The formula of medium 1 is Polygonatum sibiricum and 20% volume of distilled water;

[0128] The steps of the Cordyceps militaris-Polygonatum bidirectional solid fermentation method are as follows:

[0129] (1) Activation of Cordyceps militaris

[0130] The Cordyceps militaris strain CICC14013 was inoculated into the PDA slant medium and cultured at 28°C for 7 days. When the mycelium covered the slant, it was set aside.

[0131] Slant medium: 20% potato, 2% glucose, 0.1% KH2PO4, 0.1% MgSO4, 2.5% agar.

[0132] (2) Cordyceps militaris seed liquid culture

[0133] Mycelium was picked from the Cordyceps militaris slant culture medium and inoculated into the primary seed solution, and cultured at 28°C and 180 r / min shaking conditions for 5 days. The primary culture solution was inoculated with the secondary seed solution at a 5% inoculation rate, and then placed in a constant temperature shaker for further culture for 5 days to prepare the Cordyceps militaris seed solution.

[0134] Shake flask seed culture medium: 2% glucose, 1% peptone, 0.05% KH2PO4, 0.05% MgSO4.

[0135] (3) Cordyceps militaris-Polygonatum bidirectional solid fermentation culture

[0136] Weigh Polygonatum sibiricum, crush it into 100 mesh size, mix it evenly with 20% volume of distilled water, sterilize it once by high pressure at 121°C for 30 min, shake it loose and set it aside.

[0137] The mycelium in the secondary seed liquid of Cordyceps militaris is collected on a sterile filter membrane by a vacuum filtration method, and then mixed with a polygonatum sibiricum matrix at a volume ratio of 20%. The culture is placed in the dark under the conditions of 28° C. and a relative humidity of 50%, and when the mycelium of Cordyceps militaris covers the entire medicinal matrix and forms an obvious fungus bud structure, the fermentation is terminated to obtain a Cordyceps militaris-polygonatum sibiricum fermentation culture.

[0138] The Cordyceps militaris-Polygonatum fermented culture was placed in a hot air circulation drying oven and dried at 50°C to a constant weight. After drying, it was crushed into 80 meshes, packaged and stored in a desiccator.

[0139] Comparative Example 1

[0140] The same as Example 1, except that the formula of the culture medium 2 is Polygonatum sibiricum and 20% volume of nutrient solution, wherein the formula of the nutrient solution is 2% glucose, 2% yeast extract, and 96% distilled water.

[0141] Comparative Example 2

[0142] Same as Example 1, except that the formula of culture medium 3 is Bupleurum and 20% by volume of distilled water.

[0143] Test Example 1

[0144] (1) Nutritional components of Cordyceps militaris-Polygonatum bidirectional solid fermentation products

[0145] The cordycepin and cordyceps-polygonatum polysaccharide in the Cordyceps militaris-polygonatum culture dry powder obtained in Example 1 and Comparative Examples 1 and 2 were measured to compare the effects of different culture medium matrices on the content of each component of the fermentation product.

[0146] The Cordyceps militaris-Polygonatum bidirectional solid fermentation product mainly contains cordycepin and polysaccharides, among which the polysaccharides in medium 1 and medium 2 are Polygonatum cyrtonema polysaccharides, and the polysaccharides in medium 3 are Bupleurum polysaccharides. The test results of each nutrient component are shown in Table 1.

[0147] Table 1 Screening of solid fermentation medium

[0148]

[0149] It is known from the experimental data shown in Table 1 that the culture medium 1 uses Polygonatum sibiricum as a single culture medium to ferment Cordyceps militaris strains, and the obtained cordycepin content and polysaccharide content are higher than that of the culture medium 2 using Polygonatum sibiricum and nutrient solution composite culture medium to ferment Cordyceps militaris strains. This experimental data shows that the use of Polygonatum sibiricum as a single culture medium has a suitable ratio of nutrients, and the addition of additional nutrients to the Polygonatum sibiricum culture medium will inhibit the bidirectional solid fermentation of Cordyceps militaris-Polygonatum sibiricum. It may be due to the high sugar concentration in the culture medium that the edible fungi preferentially use sugar for metabolism instead of converting substrates, resulting in a slower fermentation rate.

[0150] The experimental data show that compared with the culture medium 1 using Polygonatum sibiricum as the single culture medium for fermenting Cordyceps militaris, the culture medium 3 using Bupleurum chinense as the single culture medium for fermenting Cordyceps militaris, the obtained cordycepin and polysaccharide contents are significantly higher.

[0151] In order to more clearly compare the effects of using Polygonatum sibiricum and Bupleurum chinense as single culture medium on the fermentation of Cordyceps militaris, the present invention tested the initial polysaccharide content of Polygonatum sibiricum and Bupleurum chinense, as shown in Table 2.

[0152] Table 2 Initial polysaccharide content of Polygonatum sibiricum and Bupleurum chinense

[0153] index Polygonatum Bupleurum Polysaccharide (mg / g) 11.34±0.23 15.38±0.16

[0154] The test showed that the initial polysaccharide content of Polygonatum sibiricum was lower than that of Bupleurum chinense. This experimental data shows that using Polygonatum sibiricum as a single culture medium to ferment Cordyceps militaris has a better effect on the proliferation of cordycepin and polysaccharides than using Bupleurum chinense as a single culture medium to ferment Cordyceps militaris.

[0155] (2) Efficacy test of Cordyceps militaris-Polygonatum bidirectional solid fermentation products

[0156] Animal experiments were carried out on the Cordyceps militaris-Polygonatum solid fermentation products obtained in Example 1 and Comparative Examples 1 and 2 to explore the effects of the Cordyceps militaris-Polygonatum solid fermentation products obtained after inoculation and culture with Cordyceps militaris on the changes in the levels of serum cholesterol, triglycerides, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in mice; and to explore the changes in the levels of serum immunoglobulin G, immunoglobulin M, cytokine IL-2, and cytokine IL-6 in mice.

[0157] The effects of Cordyceps militaris-Polygonatum solid fermentation on enhancing immunity and regulating blood lipids in mice are shown in Table 3.

[0158] Table 3 Effects of Cordyceps militaris-Polygonatum sibiricum fermentation culture on various indicators of hyperlipidemic mice

[0159] index Control group Cordyceps militaris and Polygonatum cyrtonema fermentation culture group Serum cholesterol (mmol / L) 8.42±0.04 6.48±0.02 Triglyceride (mmol / L) 2.48±0.02 2.14±0.02 Low-density lipoprotein cholesterol (mmol / L) 1.67±0.03 1.37±0.03 High-density lipoprotein cholesterol (mmol / L) 1.53±0.03 1.83±0.04 Immunoglobulin G (mg / mL) 8.38±2.47 9.43±2.04 Immunoglobulin M (μg / mL) 458±122.17 538±157.28 Cytokine IL-2 (pg / mL) 203.26±25.41 228.42±21.37 Interleukin IL-6 (pg / mL) 65.83±0.72 73.27±0.96

[0160] The experimental results show that the Cordyceps militaris-Polygonatum fermentation culture group can effectively reduce the levels of serum cholesterol, triglycerides, and low-density lipoprotein cholesterol, while increasing the content of high-density lipoprotein cholesterol; it can significantly increase the levels of immunoglobulin G and immunoglobulin M, indicating that the immune status of mice is significantly improved; it can effectively promote the secretion and expression of cytokines IL-2 and IL-6, indicating that the immune function of mice is enhanced.

[0161] In order to explore the influence of the lactic acid bacteria fermentation process on the two-step fermentation in the present invention, the following Examples 2-4 respectively use three different lactic acid bacteria, Lactobacillus rhamnosus Glory LG12, Lactobacillus paracasei Glory LP16, and Lactobacillus plantarum CCFM8661, to carry out two-step fermentation on Cordyceps militaris-Polygonatum solid fermentation culture, detect the number of lactic acid bacteria in the same fermentation time, judge the fermentation rate, and screen the dominant lactic acid bacteria strains for Cordyceps militaris-Polygonatum two-step fermentation.

[0162] Example 2 Lactic acid bacteria liquid fermentation of Cordyceps militaris-Polygonatum

[0163] The Cordyceps militaris-Polygonatum bidirectional solid fermentation culture powder obtained in Example 1 was added to a fermentation tank, and pure water was added to prepare a concentration of 10%, and then 5% of 10 7 CFU / mL of Lactobacillus rhamnosus Glory LG12 seed liquid was mixed evenly, and then cultured at 37°C for 5 days, and the fermentation was stopped when the pH reached 4.4.

[0164] Further, the steps of preparing the Cordyceps militaris-Polygonatum sibiricum two-step fermentation product are as follows:

[0165] (1) Filtration: After the fermentation of Cordyceps militaris-Polygonatum sibiricum and lactic acid bacteria is completed, use gauze to filter and remove the residue.

[0166] (2) Centrifugation: Centrifuge at 4000 r / min for 10 min to obtain the fermentation supernatant.

[0167] (3) Freeze drying: -40°C, 0.1 MPa, vacuum freeze drying for 48 h to obtain Cordyceps militaris-Polygonatum sibiricum-probiotic freeze-dried powder.

[0168] Example 3 Lactic acid bacteria liquid fermentation of Cordyceps militaris-Polygonatum

[0169] Same as Example 2, except that the lactic acid bacteria is Lactobacillus paracasei Glory LP16.

[0170] Example 4 Lactic acid bacteria liquid fermentation of Cordyceps militaris-Polygonatum

[0171] Same as Example 2, except that the lactic acid bacteria is Lactobacillus plantarum CCFM8661.

[0172] Test Example 2

[0173] (1) Nutritional components of the Cordyceps militaris-Polygonatum sibiricum two-step fermentation product

[0174] The cordycepin and Cordyceps militaris-Polygonatum polysaccharide in the Cordyceps militaris-Polygonatum culture obtained in Example 1 and the Cordyceps militaris-Polygonatum-probiotic freeze-dried powder obtained in Examples 2-4 were measured, and the changes in the content of each component after the Cordyceps militaris-Polygonatum two-step fermentation were compared.

[0175] The liquid fermentation results of Cordyceps militaris-Polygonatum solid fermentation culture by different lactic acid bacteria are shown in Table 4.

[0176] Table 4 Changes in lactic acid bacteria content after two-step fermentation of Cordyceps militaris and Polygonatum sibiricum (number of lactic acid bacteria, CFU / g)

[0177]

[0178] The experimental results showed that the three lactic acid bacteria entered the logarithmic growth phase within 4 hours, indicating that Lactobacillus rhamnosus GloryLG12, Lactobacillus paracasei Glory LP16, and Lactobacillus plantarum CCFM8661 all have relatively strong adaptability. Lactobacillus rhamnosus Glory LG12, Lactobacillus paracasei Glory LP16, and Lactobacillus plantarum CCFM8661 did not die after 28 hours of fermentation, indicating that the three strains can adapt to the Cordyceps militaris-Polygonatum two-step fermentation liquid medium.

[0179] In addition, during the same fermentation time, the number of active lactic acid bacteria of Lactobacillus plantarum CCFM8661 was higher than that of Lactobacillus rhamnosus Glory LG12 and Lactobacillus paracasei Glory LP16. And within the fermentation time of 0-28h, the number of active lactic acid bacteria of Lactobacillus plantarum CCFM8661 was always higher than that of Lactobacillus rhamnosus Glory LG12 and Lactobacillus paracasei Glory LP16, indicating that Lactobacillus plantarum CCFM8661 has more advantages in the two-step fermentation of Cordyceps militaris-Polygonatum sibiricum.

[0180] (2) Efficacy test of Cordyceps militaris-Polygonatum sibiricum two-step fermentation product

[0181] The changes in the efficacy content of Cordyceps militaris-Polygonatum sibiricum before and after the two-step fermentation are shown in Table 5.

[0182] Table 5 Changes in the content of ingredients before and after the two-step fermentation of Cordyceps militaris-Polygonatum

[0183]

[0184]

[0185] The experimental results showed that cordycepin was formed after two-way solid-state fermentation of Cordyceps militaris and Polygonatum sibiricum, and the contents of cordycepin and polysaccharides increased significantly after deep liquid fermentation by lactic acid bacteria. In particular, the cordycepin and polysaccharide contents of the solid-state fermentation culture of Cordyceps militaris and Polygonatum sibiricum fermented by CCFM8661 increased more significantly than those of the solid-state fermentation culture of Cordyceps militaris and Polygonatum sibiricum fermented by Lactobacillus rhamnosus Glory LG12 and Lactobacillus paracasei Glory LP16.

[0186] Example 5 Preparation of Cordyceps militaris-Polygonatum-probiotic granules

[0187] The formula is mainly composed of the following substances in parts by weight:

[0188] 40 portions of Cordyceps militaris-Polygonatum-probiotic freeze-dried powder prepared in Example 4,

[0189] Sugar alcohol: 38 parts of sorbitol,

[0190] Prebiotics: 20 parts of xylo-oligosaccharide,

[0191] Acidity regulator: 1 part anhydrous citric acid,

[0192] Sweetness regulator: 0.5 parts of stevioside,

[0193] Binder: 0.5 parts of sodium carboxymethyl cellulose.

[0194] The production process of Cordyceps militaris and Polygonatum sibiricum probiotic granules is as follows:

[0195] (1.1) Ingredients: Accurately weigh each material according to the product formula, and prepare the adhesive into a 3% adhesive solution using pure water.

[0196] (1.2) Mixing: Put the weighed materials except Cordyceps militaris-Polygonatum sibiricum-probiotic freeze-dried powder into a mixing tank in sequence, and mix at 600 r / min for 30 min until the mixture is uniform.

[0197] (1.3) Granulation: The mixed materials are put into a wet granulator, 5% binder solution is added, and the granulation is completed after high-speed shearing and stirring.

[0198] (1.4) Drying: Boiling drying, drying until the moisture content is less than 5%.

[0199] (1.5) Granulation: The dried material is granulated through a 14-mesh filter. The material after granulation is granular.

[0200] (1.6) Total mixing: The granular material is mixed with the Cordyceps militaris-Polygonatum sibiricum-probiotic freeze-dried powder in a mixing tank at 600 r / min for 30 min until the mixture is uniform, thereby obtaining the Cordyceps militaris-Polygonatum sibiricum-probiotic granules.

[0201] (1.7) Packaging: Fill the Cordyceps militaris-Polygonatum sibiricum-probiotic granules into aluminum foil bags, 2 g / bag.

[0202] (1.8) Metal inspection: The product is passed through a metal detector, process parameters: iron Fe<Ф1.2mm, stainless steel SUS<Ф1.2mm.

[0203] Example 6 Preparation of Cordyceps militaris-Polygonatum-probiotic tablets

[0204] The formula is mainly composed of the following substances in parts by weight:

[0205] 40 portions of Cordyceps militaris-Polygonatum-probiotic freeze-dried powder prepared in Example 4,

[0206] Sugar alcohol: 37.5 parts of sorbitol,

[0207] Prebiotics: 20 parts of xylo-oligosaccharide,

[0208] Acidity regulator: 1 part anhydrous citric acid,

[0209] Sweetness regulator: 0.5 parts of stevioside,

[0210] Binder: 0.5 parts of sodium carboxymethyl cellulose,

[0211] Anticaking agent: 0.5 parts of magnesium stearate.

[0212] The production process of Cordyceps militaris-Polygonatum-probiotic tablets is as follows:

[0213] (1.1) Ingredients: Accurately weigh each material according to the product formula, and prepare the adhesive into a 3% adhesive solution using pure water.

[0214] (1.2) Mixing: Put all the materials weighed except the Cordyceps militaris-Polygonatum sibiricum probiotic freeze-dried powder into a mixing tank in sequence, and mix at 600 r / min for 30 min until the mixture is uniform.

[0215] (1.3) Granulation: The mixed materials are put into a wet granulator, 5% binder solution is added, and the granulation is completed after high-speed shearing and stirring.

[0216] (1.4) Drying: Boiling drying, drying until the moisture content is less than 5%.

[0217] (1.5) Granulation: The dried material is granulated through a 16-mesh filter. The material after granulation is granular.

[0218] (1.6) Total mixing: The granular material is mixed with the Cordyceps militaris-Polygonatum sibiricum probiotic freeze-dried powder in a mixing tank at 600 r / min for 30 min until the mixture is uniform, thereby obtaining the Cordyceps militaris-Polygonatum sibiricum-probiotic granules.

[0219] (1.7) Tablet pressing: put the Cordyceps militaris-Polygonatum sibiricum-probiotic granules into a tablet press and compress them into tablets at 1 g / tablet.

[0220] (1.8) Packaging: Bottle the Cordyceps militaris-Polygonatum-probiotic tablets, 60 tablets / bottle.

[0221] (1.9) Metal inspection: The product is passed through a metal detector, process parameters: iron Fe<Ф1.2mm, stainless steel SUS<Ф1.2mm.

[0222] Example 7 Preparation of Cordyceps militaris-Polygonatum-Probiotic Capsules

[0223] The formula is mainly composed of the following materials in parts by weight:

[0224] 40 portions of Cordyceps militaris-Polygonatum-probiotic freeze-dried powder prepared in Example 4,

[0225] Prebiotics: 58 parts of xylo-oligosaccharide,

[0226] Anticaking agent: 2 parts of magnesium stearate.

[0227] The production process of Cordyceps militaris-Polygonatum-probiotic capsules is as follows:

[0228] (1.1) Ingredients: Accurately weigh each material according to the product formula.

[0229] (1.2) Mixing: Put the weighed materials into the mixing tank in turn, mix at 600r / min for 30min until they are evenly mixed.

[0230] (1.3) Canning: Use 0# capsule shell, put the mixed material into the capsule machine, and can 0.5g / capsule.

[0231] (1.4) Packaging: Bottle the Cordyceps militaris and Polygonatum sibiricum probiotic capsules, 60 capsules / bottle.

[0232] (1.5) Metal inspection: The product is passed through a metal detector, process parameters: iron Fe<Ф1.2mm, stainless steel SUS<Ф1.2mm.

[0233] Test Example 3

[0234] The Cordyceps militaris-Polygonatum-probiotic granules, Cordyceps militaris-Polygonatum probiotic tablets, and Cordyceps militaris-Polygonatum probiotic capsules prepared by the two-step fermentation of Examples 5-7 were given to mice for consumption, and animal experiments were conducted to investigate the effects of the three products on the changes in serum cholesterol, triglycerides, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol levels in mice; and to investigate the effects of the three products on the changes in serum immunoglobulin G, immunoglobulin M, cytokine IL-2, and cytokine IL-6 levels in mice.

[0235] The effects of consuming the three products on various indicators of hyperlipidemic mice are shown in Table 6.

[0236] Table 6 Effects of consuming three products on various indicators of hyperlipidemic mice

[0237]

[0238] The experimental results showed that Cordyceps sinensis and Polygonatum probiotic granules, Cordyceps sinensis and Polygonatum probiotic tablets, and Cordyceps sinensis and Polygonatum probiotic capsules can effectively reduce the levels of serum cholesterol, triglycerides, and low-density lipoprotein cholesterol, and increase the level of high-density lipoprotein cholesterol; can significantly increase the levels of immunoglobulin G and immunoglobulin M, indicating that the immune status of mice has been significantly improved; can effectively promote the secretion and expression of cytokines IL-2 and IL-6, indicating that the immune function of mice has been enhanced. Among them, the changes in various indicators of mice eating Cordyceps sinensis and Polygonatum probiotic capsules were the most significant.

[0239] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A two-step fermentation method for preparing functional food for enhancing immunity and regulating blood lipids, characterized in that: The following steps are involved: S1, the activated Cordyceps militaris is fermented and cultured in a fermentation medium with Polygonatum sibiricum as the only matrix source, and the Cordyceps militaris mycelium and Polygonatum sibiricum are subjected to a two-way solid fermentation to obtain a Cordyceps militaris-Polygonatum sibiricum solid fermentation product; S2. The obtained Cordyceps militaris-Polygonatum solid fermentation product is subjected to lactic acid bacteria fermentation to obtain Cordyceps militaris-Polygonatum-probiotic fermentation product.

2. The method according to claim 1, characterized in that In step S1, the fermentation medium consists of Polygonatum sibiricum and 20-40% volume of water; and / or Preferably, the particle size of the polygonatum is 50-100 mesh; and / or Preferably, the method for preparing the fermentation medium comprises: crushing polygonatum to 50-100 mesh, mixing evenly with pure water, wherein the volume ratio of pure water to polygonatum powder is 0.2-0.4:1, and sterilizing to obtain the fermentation medium.

3. The method according to claim 1, characterized in that In step S1, in the bidirectional solid fermentation, the inoculation amount of Cordyceps militaris is 2-5%.

4. The method according to claim 1, characterized in that: In step S1, the bidirectional solid fermentation includes culturing at 24-28°C and 30-50% relative humidity in the dark.

5. The method according to claim 1, characterized in that In step S2, the Cordyceps militaris-Polygonatum solid fermentation product is dried and crushed before being fermented by lactic acid bacteria. Preferably, the Cordyceps militaris-Polygonatum solid fermentation product is crushed to 50-100 mesh; preferably, the drying comprises drying at 50° C.-65° C. to constant weight; and / or Preferably, in step S2, in the lactic acid bacteria fermentation system, the mass concentration of the Cordyceps militaris-Polygonatum sibiricum solid fermentation product is 10-30%, and the inoculation amount of lactic acid bacteria is 2%-5%; and / or Preferably, in step S2, in the lactic acid bacteria fermentation system, the lactic acid bacteria include one or more of Lactobacillus rhamnosus Glory LG12, Lactobacillus paracasei Glory LP16, and Lactobacillus plantarum CCFM8661, preferably Lactobacillus plantarum CCFM8661; and / or Preferably, in step S2, the conditions for lactic acid bacteria fermentation include culturing at 37° C. for 3-5 days and stopping the fermentation when the pH reaches 4.

4.

6. The method according to claim 1, characterized in that The Cordyceps militaris-Polygonatum-probiotic fermentation product obtained in step S2 is further filtered and freeze-dried to obtain Cordyceps militaris-Polygonatum-probiotic freeze-dried powder.

7. A Cordyceps militaris-Polygonatum-probiotic fermentation product prepared by the method according to any one of claims 1 to 6.

8. The Cordyceps militaris-Polygonatum-probiotic fermentation product according to claim 7, characterized in that: The Cordyceps militaris-Polygonatum-probiotic fermentation product has the ability to enhance immunity and regulate blood lipids; and / or Preferably, the Cordyceps militaris-Polygonatum-probiotic fermentation product contains more than 8.0 mg / g of cordycepin and more than 30.0 mg / g of Cordyceps militaris-Polygonatum polysaccharide; and / or Preferably, the Cordyceps militaris-Polygonatum-probiotic fermentation product contains more than 10.0 mg / g of cordycepin and more than 31.0 mg / g of Cordyceps militaris-Polygonatum polysaccharide; and / or Preferably, the Cordyceps militaris-Polygonatum-probiotic fermentation product contains more than 13.0 mg / g of cordycepin and more than 35.0 mg / g of Cordyceps militaris-Polygonatum polysaccharide.

9. A composition comprising the Cordyceps militaris-Polygonatum sibiricum-probiotic fermentation product according to claim 7 or 8; Preferably, the composition further comprises a pharmaceutically acceptable carrier or excipient; Preferably, the composition is in the form of granules, tablets or capsules.

10. Use of the Cordyceps militaris-Polygonatum-probiotic fermentation product according to claim 7 or 8, or the composition according to claim 9 in food or medicine.

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