Composition for repairing sensitive skin as well as preparation method and application thereof in cosmetics

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a composition for repairing sensitive skin, a preparation method of the composition and application of the composition in cosmetics. The collagen, the soluble collagen, the hydrolyzed collagen, the madecassoside, the albizia flower extract, the radix saposhnikoviae extract, the yeast fermentation lysate filtrate, the bifidus yeast fermentation product filtrate, the bacillus fermentation product, the carnosine, the polygonum cuspidatum root extract, the ganoderma atrum extract and other raw materials are used as active ingredients and are mutually matched and jointly act; the composition is endowed with excellent safety, anti-inflammatory repair and moisturizing effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a composition for repairing sensitive skin, a preparation method thereof, and an application of the composition in cosmetics. Background Art

[0002] The main causes of sensitive skin include damaged or destroyed skin barrier function and external stimuli. Sensitive skin is generally divided into congenital and acquired types. ‌Congenital sensitive skin‌: mostly inherited, the skin is naturally thinner, with obvious red blood vessels, and easily affected by changes in the external environment. ‌Acquired sensitive skin‌: due to some stimulation or improper skin care behaviors, the skin becomes sensitive and fragile, which can cause the sebum membrane of the outermost layer of the skin to be damaged, the skin's resistance to decrease, the stratum corneum to become thinner, and it cannot play a normal protective role. ‌ ‌Repairing sensitive skin‌ means improving and repairing sensitive skin through a series of skin care measures to restore it to a healthy state. Currently, there are many types of cosmetics for repairing sensitive skin on the market, but the quality is uneven. Although they have a certain soothing and repairing effect, they lack moisturizing and other properties. Therefore, providing a composition with high safety and excellent performance in repairing sensitive skin and moisturizing the skin is a technical problem that needs to be solved at present. Summary of the invention

[0003] In view of the above technical problems, the present invention provides a composition for repairing sensitive skin, a preparation method thereof, and application in cosmetics.

[0004] On the one hand, the present invention provides a composition for repairing sensitive skin, the raw materials of which are: collagen, soluble collagen, hydrolyzed collagen, madecassoside, Albizzia Julibrissin flower extract, Saposhnikovia root extract, yeast fermentation lysate filtrate, bifid yeast fermentation product filtrate, Bacillus fermentation product, carnosine, Polygonum cuspidatum root extract and Ganoderma lucidum extract. The above raw materials interact with each other and cooperate together to give the composition excellent safety, sensitive skin repair and moisturizing effects.

[0005] Preferably, the composition comprises the following raw materials in parts by mass: 0.001-0.2 parts of collagen, 0.001-0.1 parts of soluble collagen, 0.001-0.2 parts of hydrolyzed collagen, 0.05-1 parts of madecassoside, 0.005-0.04 parts of Albizzia Julibrissin flower extract, 0.05-1 parts of Saposhnikovia root extract, 0.05-0.8 parts of yeast fermentation lysate filtrate, 0.01-1 parts of Bifida fermentation product filtrate, 0.01-0.3 parts of Bacillus fermentation product, 0.001-0.1 parts of carnosine, 0.01-0.3 parts of Polygonum cuspidatum root extract and 0.05-1 parts of Ganoderma lucidum extract.

[0006] Preferably, the Albizzia Julibrissin flower extract and Polygonum cuspidatum root extract can be obtained by conventional extraction methods, including but not limited to: crushing the Albizzia Julibrissin flower or Polygonum cuspidatum root, then mixing with water at a mass ratio of 1:10-50, heating and stirring to extract, filtering, and drying.

[0007] Preferably, the extraction method of the radix silergii extract comprises: crushing the radix silergii, then mixing it with 20-35wt% ethanol aqueous solution at a mass ratio of 1:10-50, heating and stirring at 45-60°C for 5-12 hours, filtering, and drying the filtrate to obtain the radix silergii extract.

[0008] Preferably, the extraction method of the black ganoderma extract comprises: crushing the black ganoderma to obtain a crushed material; adding 50-75wt% ethanol aqueous solution with a mass of 10-30 times the mass of the crushed material, heating and stirring at 50-60°C for 2-5 hours, and then filtering to obtain a filtrate; concentrating the filtrate under reduced pressure at 50-60°C to a mass of 0.8-1.5 times the mass of the crushed material to obtain a concentrate; mixing the concentrate with an extractant composed of water and an organic solvent in a mass ratio of 1:2-4, the organic solvent being ethyl acetate and chloroform in a mass ratio of 2-3.5:1, and then stirring and extracting at a temperature of 30-40°C for 2-4 hours, then standing and stratifying, and concentrating the oil phase obtained by stratification under reduced pressure until ethyl acetate and chloroform are undetectable, stopping the reduced pressure distillation, and obtaining the black ganoderma extract.

[0009] Preferably, the mass ratio of madecassoside to the black ganoderma extract is 0.4-0.8:1.

[0010] Preferably, the mass ratio of the radix silergii extract to the filtrate of the yeast fermentation lysate is 1:1-1.5.

[0011] Preferably, the composition comprises the following raw materials in parts by mass: 0.005-0.15 parts of collagen, 0.005-0.08 parts of soluble collagen, 0.005-0.1 parts of hydrolyzed collagen, 0.08-0.9 parts of madecassoside, 0.01-0.03 parts of Albizzia Julibrissin flower extract, 0.08-0.9 parts of Saposhnikovia root extract, 0.08-0.75 parts of yeast fermentation lysate filtrate, 0.05-0.5 parts of Bifida fermentation product filtrate, 0.05-0.25 parts of Bacillus fermentation product, 0.005-0.08 parts of carnosine, 0.05-0.2 parts of Polygonum cuspidatum root extract and 0.08-0.9 parts of Ganoderma lucidum extract.

[0012] Preferably, the composition may further include 0.1-5 parts by mass of butanediol.

[0013] Preferably, the composition may further include 5-50 parts of water by weight.

[0014] On the other hand, the present invention also provides a method for preparing the composition, comprising stirring and mixing yeast fermentation lysate filtrate, bifid yeast fermentation product filtrate, black ganoderma lucidum extract, and radix siler extract, then adding the remaining raw materials and continuing stirring and sterilizing to obtain the composition.

[0015] On the other hand, the present invention also provides use of the composition in the preparation of cosmetics, wherein the amount of the composition in the cosmetics is 0.1-30wt%.

[0016] Preferably, the dosage form of the cosmetic includes aqueous solution, emulsion, ointment, cream, and powder.

[0017] Preferably, the cosmetics include facial masks, ointments, creams, lotions, essences, foundations, eye shadows, blushers, shampoos, perfumes, and the like.

[0018] Beneficial effects of the present invention: The present invention adopts collagen, soluble collagen, hydrolyzed collagen, madecassoside, Albizzia julibrissin flower extract, Saposhnikovia root extract, yeast fermentation lysate filtrate, bifid yeast fermentation product filtrate, Bacillus fermentation product, carnosine, Polygonum cuspidatum root extract and black ganoderma lucidum extract as active ingredients, which cooperate with each other and work together to give the composition excellent performance.

[0019] The composition prepared by the invention has no irritation to the skin, high safety, and excellent anti-inflammatory and skin repairing effects.

[0020] The present invention adopts a combination of ethyl acetate and chloroform as the organic solvent in the extractant, and compared with using one of them or replacing it with other organic solvents, the black ganoderma lucidum is extracted, and the prepared black ganoderma lucidum extract has better anti-inflammatory effect and can give the composition better anti-inflammatory and repair performance; during the extraction of the present invention, ethyl acetate and chloroform are used together to produce a mutually reinforcing extraction effect, thereby improving the anti-inflammatory performance of the black ganoderma lucidum extract.

[0021] The invention adopts madecassoside and black ganoderma extract to work together to synergistically enhance the anti-inflammatory performance of the composition. The mass ratio of madecassoside and black ganoderma extract used in the invention is 0.4-0.8:1, and the obtained composition has better anti-inflammatory performance.

[0022] The composition prepared by the invention can effectively increase the water content of the skin and has a good moisturizing and emollient effect.

[0023] The moisturizing effect of the radix silergi prepared by the present invention using high-concentration ethanol solution extraction is relatively poor. The radix silergi extract prepared by the present invention using 20-35wt% ethanol aqueous solution extraction has better moisturizing performance.

[0024] The present invention uses a combination of a radix fangfeng extract and a yeast fermentation lysate filtrate to mutually promote the moisturizing and emollient properties of the composition. When the mass ratio of the radix fangfeng extract to the yeast fermentation lysate filtrate is 1:1-1.5, the obtained composition has better moisturizing and emollient properties.

[0025] The product of the invention has excellent effect, simple preparation method and good application prospect. DETAILED DESCRIPTION

[0026] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are used to illustrate the present invention, rather than to limit the present invention.

[0027] The raw material information of the specific implementation mode of the present invention is as follows: The extraction method of the Albizzia julibrissin flower extract is as follows: the dried Albizzia julibrissin flower is crushed, then mixed with water in a mass ratio of 1:35, heated to 60°C and stirred at 60 rpm for 5 hours, filtered, and freeze-dried at -40°C to a water content of 3.62wt% to obtain the Albizzia julibrissin flower extract.

[0028] Extraction method of Polygonum cuspidatum root extract: grind the dried Polygonum cuspidatum root, mix it with water in a mass ratio of 1:28, heat it to 62°C, stir it at 70 rpm for 4.8 hours, filter it, and dry it at -42°C to a water content of 3.02wt% to obtain the Polygonum cuspidatum root extract.

[0029] Other raw material information is shown in Table 1.

[0030] Table 1: Raw material information

[0031] 1. Preparation of products Composition 1: According to mass parts, the raw materials are composed of: 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 parts of hydrolyzed collagen, 0.35 parts of madecassoside, 0.03 parts of Albizia Julibrissin flower extract, 0.5 parts of Saposhnikovia root extract, 0.6 parts of yeast fermentation lysate filtrate, 0.08 parts of Bifida fermentation product filtrate, 0.09 parts of Bacillus fermentation product, 0.02 parts of carnosine, 0.1 parts of Polygonum cuspidatum root extract, 0.5 parts of black ganoderma lucidum extract, 3 parts of 1,3-butylene glycol, and water makes up to 40 parts.

[0032] Extraction method of fangfeng root extract: the dried fangfeng root is crushed to a particle size less than 20 mesh, and then mixed with 25wt% ethanol aqueous solution in a mass ratio of 1:35, heated at 60°C and stirred at 70rpm for 10 hours, filtered through a 0.2 micron pore size filter membrane, and the filtrate is freeze-dried at -42°C to a water content of 2.50wt% to obtain the fangfeng root extract.

[0033] The extraction method of black ganoderma lucidum extract comprises the following steps: crushing dried black ganoderma lucidum to a particle size of less than 20 meshes to obtain a crushed material; adding a 65wt% ethanol aqueous solution of 25 times the mass of the crushed material, heating at 55°C and stirring at 70rpm for 3.5 hours, and then filtering with a 0.2-micron pore size filter membrane to obtain a filtrate; concentrating the filtrate under reduced pressure at 55°C and 0.005MPa to a mass of 1 times the mass of the crushed material to obtain a concentrate; mixing the concentrate with an extractant composed of water and an organic solvent in a mass ratio of 1:3, wherein the organic solvent is ethyl acetate and chloroform in a mass ratio of 3:1, and then extracting under stirring at 70rpm at a temperature of 35°C for 3 hours, and then standing for stratification for 2 hours, and concentrating the oil phase obtained by stratification under reduced pressure at 40°C and 0.002Mpa until no ethyl acetate and chloroform can be detected, and stopping the reduced pressure distillation to obtain the black ganoderma lucidum extract.

[0034] The preparation method of the composition is as follows: The yeast fermentation lysate filtrate, the bifid yeast fermentation product filtrate, the black ganoderma lucidum extract and the radix siler extract were stirred and mixed at 80 rpm for 0.3 hour, and then the remaining raw materials were added and stirred at 80 rpm for 0.5 hour for sterilization to obtain the composition.

[0035] Composition 2: According to mass parts, the raw materials are composed of: 0.06 parts of collagen, 0.01 parts of soluble collagen, 0.04 parts of hydrolyzed collagen, 0.29 parts of madecassoside, 0.029 parts of Albizia Julibrissin flower extract, 0.45 parts of Saposhnikovia root extract, 0.65 parts of yeast fermentation lysate filtrate, 0.07 parts of Bifida fermentation product filtrate, 0.11 parts of Bacillus fermentation product, 0.012 parts of carnosine, 0.09 parts of Polygonum cuspidatum root extract, 0.56 parts of Ganoderma lucidum extract, 3 parts of 1,3-butylene glycol, and water makes up to 40 parts.

[0036] Extraction method of fangfeng root extract: the dried fangfeng root is crushed to a particle size less than 20 mesh, and then mixed with 20wt% ethanol aqueous solution in a mass ratio of 1:45, heated at 45°C and stirred at 80rpm for 9.5 hours, filtered through a 0.2-micron pore size filter membrane, and the filtrate is freeze-dried at -42°C to a water content of 2.47wt% to obtain the fangfeng root extract.

[0037] The extraction method of black ganoderma lucidum extract comprises the following steps: crushing dried black ganoderma lucidum to a particle size of less than 20 meshes to obtain a crushed material; adding a 75wt% ethanol aqueous solution of 25 times the mass of the crushed material, heating at 52°C and stirring at 70rpm for 4 hours, and then filtering with a 0.2-micron pore size filter membrane to obtain a filtrate; concentrating the filtrate under reduced pressure at 55°C and 0.005MPa to a mass of 1 times the mass of the crushed material to obtain a concentrate; mixing the concentrate with an extractant composed of water and an organic solvent in a mass ratio of 1:2, wherein the organic solvent is ethyl acetate and chloroform in a mass ratio of 3.5:1, and then extracting under stirring at 70rpm at a temperature of 38°C for 3.5 hours, and then standing for stratification for 2.5 hours, and concentrating the oil phase obtained by stratification under reduced pressure at 40°C and 0.002Mpa until no ethyl acetate and chloroform are detected, and stopping the reduced pressure distillation to obtain the black ganoderma lucidum extract.

[0038] The preparation method of the composition is as follows: The yeast fermentation lysate filtrate, the bifid yeast fermentation product filtrate, the black ganoderma lucidum extract and the radix siler extract were stirred and mixed at 90 rpm for 0.25 hours, and then the remaining raw materials were added and stirred at 100 rpm for 0.4 hours for sterilization to obtain the composition.

[0039] Composition 3: According to mass parts, the raw materials are composed of: 0.07 parts of collagen, 0.015 parts of soluble collagen, 0.025 parts of hydrolyzed collagen, 0.37 parts of madecassoside, 0.032 parts of Albizzia Julibrissin flower extract, 0.55 parts of Saposhnikovia root extract, 0.55 parts of yeast fermentation lysate filtrate, 0.09 parts of Bifida fermentation product filtrate, 0.07 parts of Bacillus fermentation product, 0.03 parts of carnosine, 0.12 parts of Polygonum cuspidatum root extract, 0.48 parts of Ganoderma lucidum extract, 3 parts of 1,3-butylene glycol, and water makes up to 40 parts.

[0040] Extraction method of fangfeng root extract: the dried fangfeng root is crushed to a particle size less than 20 mesh, and then mixed with 35wt% ethanol aqueous solution in a mass ratio of 1:20, heated at 45°C and stirred at 80rpm for 12 hours, filtered through a 0.2 micron pore size filter membrane, and the filtrate is freeze-dried at -42°C to a water content of 2.61wt% to obtain the fangfeng root extract.

[0041] The extraction method of black ganoderma lucidum extract comprises the following steps: crushing dried black ganoderma lucidum to a particle size of less than 20 meshes to obtain a crushed material; adding a 50wt% ethanol aqueous solution with a mass of 20 times that of the crushed material, heating at 57°C and stirring at 70rpm for 2.5 hours, and then filtering with a 0.2-micron pore size filter membrane to obtain a filtrate; concentrating the filtrate under reduced pressure at 55°C and 0.005MPa to a mass of 0.9 times that of the crushed material to obtain a concentrate; mixing the concentrate with an extractant composed of water and an organic solvent in a mass ratio of 1:4, wherein the organic solvent is ethyl acetate and chloroform in a mass ratio of 2:1, and then extracting under a temperature of 33°C and stirring at 70rpm for 2.5 hours, and then standing for stratification for 2.5 hours, and concentrating the oil phase obtained by stratification under reduced pressure at 40°C and 0.002Mpa until no ethyl acetate and chloroform are detected, and stopping the reduced pressure distillation to obtain the black ganoderma lucidum extract.

[0042] The preparation method of the composition is as follows: The yeast fermentation lysate filtrate, the bifid yeast fermentation product filtrate, the black ganoderma lucidum extract and the radix siler extract were stirred and mixed at 75 rpm for 0.35 hours, and then the remaining raw materials were added and stirred at 95 rpm for 0.55 hours for sterilization to obtain the composition.

[0043] Composition 4: The difference compared to composition 1 is that the extraction method of the radix fangfeng extract has changed, and the concentration of the ethanol aqueous solution used in the extraction has increased. Specifically, the dried radix fangfeng was crushed to a particle size of less than 20 mesh, and then mixed with a 65wt% ethanol aqueous solution at a mass ratio of 1:35, heated at 60°C and stirred at 70rpm for 10 hours, filtered with a 0.2 micron pore size filter membrane, and the filtrate was freeze-dried at -42°C to a water content of 2.50wt% to obtain a radix fangfeng extract. The rest is the same as composition 1.

[0044] Composition 5: The difference from composition 1 is that the extraction method of the black ganoderma extract is changed, and the organic solvent is cyclohexane to replace the ethyl acetate and chloroform of composition 1. Specifically: The dried black ganoderma lucidum was crushed to a particle size of less than 20 meshes to obtain a crushed material; a 65wt% ethanol aqueous solution of 25 times the mass of the crushed material was added, heated at 55°C and stirred at 70rpm for 3.5 hours, and then filtered with a 0.2 micron pore size filter membrane to obtain a filtrate; the filtrate was concentrated under reduced pressure at 55°C and 0.005MPa to a mass of 1 times that of the crushed material to obtain a concentrate; the concentrate was mixed with an extractant composed of water and an organic solvent in a mass ratio of 1:3, the organic solvent was cyclohexane, and then extracted at 35°C and 70rpm for 3 hours, and then allowed to stand for stratification for 2 hours, and the oil phase obtained by stratification was concentrated under reduced pressure at 40°C and 0.002Mpa until no cyclohexane was detected, and the reduced pressure distillation was stopped to obtain a black ganoderma lucidum extract.

[0045] The rest is the same as composition 1.

[0046] Composition 6: The difference from composition 1 is that the extraction method of the black ganoderma extract is changed, and only ethyl acetate is used as the organic solvent. Specifically: The dried black ganoderma lucidum was crushed to a particle size of less than 20 meshes to obtain a crushed material; a 65wt% ethanol aqueous solution of 25 times the mass of the crushed material was added, heated at 55°C and stirred at 70rpm for 3.5 hours, and then filtered with a 0.2 micron pore size filter membrane to obtain a filtrate; the filtrate was concentrated under reduced pressure at 55°C and 0.005MPa to a mass of 1 times that of the crushed material to obtain a concentrate; the concentrate was mixed with an extractant composed of water and an organic solvent in a mass ratio of 1:3, the organic solvent was ethyl acetate, and then extracted at 35°C and 70rpm for 3 hours, and then allowed to stand for stratification for 2 hours, and the oil phase obtained by stratification was concentrated under reduced pressure at 40°C and 0.002Mpa until no ethyl acetate was detected, and the reduced pressure distillation was stopped to obtain a black ganoderma lucidum extract.

[0047] The rest is the same as composition 1.

[0048] Composition 7: The difference from composition 1 is that the extraction method of the black ganoderma extract is changed, and only chloroform is used as the organic solvent. Specifically: The dried black ganoderma lucidum was crushed to a particle size of less than 20 meshes to obtain a crushed material; a 65wt% ethanol aqueous solution of 25 times the mass of the crushed material was added, heated at 55°C and stirred at 70rpm for 3.5 hours, and then filtered using a 0.2 micron pore size filter membrane to obtain a filtrate; the filtrate was concentrated under reduced pressure at 55°C and 0.005MPa to a mass of 1 times the mass of the crushed material to obtain a concentrate; the concentrate was mixed with an extractant composed of water and an organic solvent in a mass ratio of 1:3, the organic solvent was chloroform, and then extracted at 35°C and 70rpm for 3 hours, and then allowed to stand for stratification for 2 hours, and the oil phase obtained by stratification was concentrated under reduced pressure at 40°C and 0.002Mpa until no chloroform was detected, and the reduced pressure distillation was stopped to obtain a black ganoderma lucidum extract.

[0049] The rest is the same as composition 1.

[0050] Composition 8: The difference from composition 1 is that madecassoside is not used in the composition formula, and the amount of black ganoderma extract is 0.85 parts. Specifically: 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 parts of hydrolyzed collagen, 0.03 parts of Albizzia Julibrissin extract, 0.5 parts of Saposhnikovia root extract, 0.6 parts of yeast fermentation lysate filtrate, 0.08 parts of bifid yeast fermentation product filtrate, 0.09 parts of Bacillus fermentation product, 0.02 parts of carnosine, 0.1 parts of Polygonum cuspidatum root extract, 0.85 parts of black ganoderma extract, 3 parts of 1,3-butylene glycol, and water is added to 40 parts.

[0051] The rest is the same as composition 1.

[0052] Composition 9: The difference compared to composition 1 is that the black ganoderma extract is not used in the composition formula, and the amount of madecassoside is 0.85 parts. Specifically: 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 parts of hydrolyzed collagen, 0.85 parts of madecassoside, 0.03 parts of Albizzia Julibrissin extract, 0.5 parts of Saposhnikovia root extract, 0.6 parts of yeast fermentation lysate filtrate, 0.08 parts of bifida fermentation product filtrate, 0.09 parts of Bacillus fermentation product, 0.02 parts of carnosine, 0.1 parts of Polygonum cuspidatum root extract, 3 parts of 1,3-butylene glycol, and water is supplemented to 40 parts.

[0053] The rest is the same as composition 1.

[0054] Composition 10: The difference from composition 1 is that the formula of the composition does not use the extract of radix fangfeng, and the amount of yeast fermentation lysate filtrate is 1.1 parts. Specifically: 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 parts of hydrolyzed collagen, 0.35 parts of madecassoside, 0.03 parts of Albizzia julibrissin extract, 1.1 parts of yeast fermentation lysate filtrate, 0.08 parts of bifida fermentation product filtrate, 0.09 parts of Bacillus fermentation product, 0.02 parts of carnosine, 0.1 parts of Polygonum cuspidatum root extract, 0.5 parts of black ganoderma extract, 3 parts of 1,3-butylene glycol, and water is added to 40 parts.

[0055] The rest is the same as composition 1.

[0056] Composition 11: The difference from composition 1 is that the composition formula does not use yeast fermentation lysate filtrate, and the amount of fangfeng root extract is 1.1 parts. Specifically: 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 parts of hydrolyzed collagen, 0.35 parts of madecassoside, 0.03 parts of Albizzia Julibrissin extract, 1.1 parts of fangfeng root extract, 0.08 parts of bifida fermentation product filtrate, 0.09 parts of Bacillus fermentation product, 0.02 parts of carnosine, 0.1 parts of Polygonum cuspidatum root extract, 0.5 parts of black ganoderma extract, 3 parts of 1,3-butylene glycol, and water is supplemented to 40 parts.

[0057] The rest is the same as composition 1.

[0058] Composition 12: The difference compared to composition 1 is that the dosage ratio of madecassoside and black ganoderma extract in the composition formula has changed. Specifically: 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 parts of hydrolyzed collagen, 0.08 parts of madecassoside, 0.03 parts of Albizia Julibrissin extract, 0.5 parts of Saposhnikovia root extract, 0.6 parts of yeast fermentation lysate filtrate, 0.08 parts of bifid yeast fermentation product filtrate, 0.09 parts of Bacillus fermentation product, 0.02 parts of carnosine, 0.1 parts of Polygonum cuspidatum root extract, 0.77 parts of black ganoderma extract, 3 parts of 1,3-butanediol, and water is added to 40 parts. The mass ratio of madecassoside to black ganoderma extract is 0.104:1 The rest is the same as composition 1.

[0059] Composition 13: The difference compared to composition 1 is that the dosage ratio of the radix fangfeng extract and the yeast fermentation lysate filtrate in the composition formula has changed. Specifically: 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 parts of hydrolyzed collagen, 0.35 parts of madecassoside, 0.03 parts of Albizzia julibrissin extract, 0.1 parts of radix fangfeng extract, 1 parts of yeast fermentation lysate filtrate, 0.08 parts of bifida yeast fermentation filtrate, 0.09 parts of Bacillus fermentation product, 0.02 parts of carnosine, 0.1 parts of Polygonum cuspidatum root extract, 0.5 parts of black ganoderma extract, 3 parts of 1,3-butanediol, and water is supplemented to 40 parts. The mass ratio of radix fangfeng extract to yeast fermentation lysate filtrate is 1:10.

[0060] The rest is the same as composition 1.

[0061] 2. Testing 1. Skin patch test Healthy volunteers aged 35-45 were selected and randomly divided into 13 groups, with 15 people in each group. The skin on the front inner side of the right arm of the volunteers was washed with water and dried naturally as the test area; then a patch tester was selected, and 0.025g of the above composition was put into the patch tester in a closed patch test manner, and applied to the test area of ​​the subject. After 24 hours, the patch tester was removed, and after another 60 minutes, the skin appearance of each group of volunteers was evaluated according to the grade standard recorded in Table 2, as shown in Table 3.

[0062] Table 2: Grade Standards

[0063] Table 3: Patch test results

[0064] According to the test results in Table 3, the composition prepared by the present invention has no irritation to the skin and is highly safe.

[0065] 2. Anti-inflammatory repair test The anti-inflammatory and skin-repairing effects of the composition were tested by measuring the level of inflammatory factor expression inhibited by the composition.

[0066] Experimental steps: HaCaT cells were cultured at 37°C and 5% CO 2 The cells were cultured in DMEM medium, and the medium was replaced every two days. When the cell confluence reached about 90%, they were applied to different groups for testing.

[0067] Group setting and drug administration setting: Blank group: The cells were cultured with DMEM medium only for 48 h.

[0068] Model group: LPS with a final concentration of 1 μg / mL was added for 24 h for stimulation, and then the medium was replaced with DMEM and cultured for another 24 h.

[0069] Composition group: LPS with a final concentration of 1 μg / mL was added for 24 h for stimulation, and then the culture medium was replaced with DMEM medium containing 8 mg / mL of composition 1-3, 5-9, 12 and cultured for another 24 h.

[0070] Positive drug group: LPS with a final concentration of 1 μg / mL was added for 24 h for stimulation, and then the medium was replaced with DMEM medium containing 9 μg / mL dexamethasone and cultured for another 24 h.

[0071] The supernatant of each group after culture was taken, and the content of inflammatory factors TNF-α and IL-1β in each group was tested using an ELISA kit according to the method described in the kit manual, and the average value was obtained by three parallel measurements; then the reduction in the content of TNF-α and IL-1β in each group compared with the model group was recorded; the units of the above four indicators were all ng / mL. The test results are shown in Table 4.

[0072] Table 4: Anti-inflammatory repair test

[0073] According to the test results in Table 4, it can be seen that the composition prepared by the present invention has excellent anti-inflammatory and skin repairing effects.

[0074] Combined with the test results of compositions 1, 5-7, it can be seen that the present invention uses a combination of ethyl acetate and chloroform as the organic solvent in the extractant to extract black ganoderma lucidum, compared with using one of them or replacing it with other organic solvents, and the prepared black ganoderma lucidum extract has better anti-inflammatory effect and can give the composition better anti-inflammatory and repair properties; ethyl acetate and chloroform are compounded together to produce a mutually reinforcing extraction effect, thereby improving the anti-inflammatory properties of the black ganoderma lucidum extract.

[0075] Combining the test results of compositions 1 and 8-9, it can be seen that when the amount of either madecassoside or black ganoderma extract in composition 8-9 is kept the same as the total amount of madecassoside and black ganoderma extract in composition 1, the anti-inflammatory effect of composition 8-9 obtained is worse than that of composition 1; thus, it can be seen that the present invention adopts madecassoside and black ganoderma extract to work together to synergistically enhance the anti-inflammatory performance of the composition.

[0076] Combined with the test results of compositions 1 and 12, it can be seen that the mass ratio of madecassoside to black ganoderma lucidum extract used in the present invention is 0.4-0.8:1, and the obtained composition has better anti-inflammatory performance.

[0077] 3. Moisturizing and emollient test 42 volunteers aged 30-50 with healthy skin were recruited and randomly divided into 7 groups, 6 people in each group. Each group of volunteers used the above-mentioned composition 1-4, 10-11, 13 twice a day on the face (once in the morning and once in the evening), each time using 0.8g, evenly applied on the face, and used continuously for 60 days. Before use and 60 days after use, CM825 was used to test the moisture content of the stratum corneum of the volunteers' cheeks (in CU). Each test selected 3 locations on the cheeks for testing, took the average value, and summarized it, and then calculated the increase in moisture content (in CU) on the 60th day compared with before use. The results are shown in Table 5.

[0078] Table 5: Moisturizing and Emollient Test Results

[0079] According to the test in Table 5, it can be seen that the composition prepared by the present invention can effectively increase the moisture content of the skin and has a good moisturizing and emollient effect.

[0080] Combining the tests of compositions 1 and 4, it can be seen that the moisturizing effect of the radix silergii prepared by extraction with high-concentration ethanol solution is relatively poor. The radix silergii extract extracted by the present invention using 20-35wt% ethanol aqueous solution has better moisturizing performance.

[0081] Combining the tests of compositions 1 and 10-11, it can be seen that when the amount of one of the radix fangfeng extract or the yeast fermentation lysate filtrate in composition 10-11 is kept the same as the total amount of the radix fangfeng extract and the yeast fermentation lysate filtrate in composition 1, the moisturizing and emollient effect of the obtained composition 10-11 is worse than that of composition 1; it can be seen that the present invention adopts a combination of radix fangfeng extract and yeast fermentation lysate filtrate to mutually promote each other to enhance the moisturizing and emollient properties of the composition.

[0082] Combined with the test results of compositions 1 and 13, it can be seen that the mass ratio of the radix silerineii extract and the yeast fermentation lysate filtrate used in the present invention is 1:1-1.5, and the resulting composition has better moisturizing and emollient properties.

[0083] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A composition for repairing sensitive skin, characterized in that: In terms of mass, the invention comprises the following raw materials: 0.001-0.2 parts of collagen, 0.001-0.1 parts of soluble collagen, 0.001-0.2 parts of hydrolyzed collagen, 0.05-1 parts of madecassoside, 0.005-0.04 parts of julibrissin flower extract, 0.05-1 parts of siler root extract, 0.05-0.8 parts of yeast fermentation lysate filtrate, 0.01-1 parts of bifid yeast fermentation product filtrate, 0.01-0.3 parts of bacillus fermentation product, 0.001-0.1 parts of carnosine, 0.01-0.3 parts of knotweed root extract and 0.05-1 parts of black ganoderma lucidum extract.

2. The composition according to claim 1, characterized in that The extraction method of the radix fangfeng extract comprises: crushing the radix fangfeng, then mixing it with 20-35wt% ethanol aqueous solution at a mass ratio of 1:10-50, heating and stirring at 45-60°C for 5-12 hours, filtering to obtain a filtrate, and drying the filtrate to obtain the radix fangfeng extract.

3. The composition according to claim 1, characterized in that The extraction method of the black ganoderma lucidum extract comprises: crushing the black ganoderma lucidum to obtain a crushed material; adding a 50-75wt% ethanol aqueous solution with a mass of 10-30 times the mass of the crushed material, heating and stirring at 50-60°C for 2-5 hours, and then filtering to obtain a filtrate; concentrating the filtrate under reduced pressure at 50-60°C to a mass of 0.8-1.5 times the mass of the crushed material to obtain a concentrate; mixing the concentrate with an extractant composed of water and an organic solvent in a mass ratio of 1:2-4, the organic solvent being ethyl acetate and chloroform in a mass ratio of 2-3.5:1, and then stirring and extracting for 2-4 hours at a temperature of 30-40°C, and then standing to separate, and concentrating the oil phase obtained by separation under reduced pressure until no ethyl acetate and chloroform can be detected, and stopping the reduced pressure distillation to obtain the black ganoderma lucidum extract.

4. The composition according to claim 1, characterized in that The mass ratio of madecassoside to black ganoderma lucidum extract is 0.4-0.8:1; the mass ratio of siler root extract to yeast fermentation lysate filtrate is 1:1-1.

5.

5. The composition according to claim 1, characterized in that In terms of mass, the invention comprises the following raw materials: 0.005-0.15 parts of collagen, 0.005-0.08 parts of soluble collagen, 0.005-0.1 parts of hydrolyzed collagen, 0.08-0.9 parts of madecassoside, 0.01-0.03 parts of julibrissin flower extract, 0.08-0.9 parts of siler root extract, 0.08-0.75 parts of yeast fermentation lysate filtrate, 0.05-0.5 parts of bifid yeast fermentation product filtrate, 0.05-0.25 parts of bacillus fermentation product, 0.005-0.08 parts of carnosine, 0.05-0.2 parts of polygonum cuspidatum root extract and 0.08-0.9 parts of black ganoderma lucidum extract.

6. The composition according to claim 1, characterized in that In terms of weight, 0.1-5 parts of butanediol are also included.

7. The composition according to claim 1, characterized in that According to the mass percentage, 5-50 parts of water are also included.

8. A method for preparing the composition according to any one of claims 1 to 7, characterized in that: The method comprises stirring and mixing yeast fermentation lysate filtrate, bifid yeast fermentation product filtrate, black ganoderma lucidum extract and radix siler extract, then adding the remaining raw materials, continuing stirring and sterilizing to obtain the composition.

9. Use of the composition according to any one of claims 1 to 7 in preparing cosmetics, characterized in that: The amount of the composition in cosmetics is 0.1-30wt%.

10. The use according to claim 9, characterized in that: The dosage forms of the cosmetics include aqueous solutions, emulsions, ointments, creams, and powders.

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