Traditional Chinese medicine composition with anti-aging and anti-oxidation functions as well as preparation and application of traditional Chinese medicine composition

Abstract

The invention discloses a traditional Chinese medicine composition with anti-aging and anti-oxidation functions as well as preparation and application thereof, and belongs to the technical field of traditional Chinese medicines. The composition improves physiological and metabolic states of old people through multi-way effects, including improving digestive function, regulating vitamin and trace element metabolism, enhancing antioxidant ability, regulating expression of senescence-related protein, improving anti-stress ability, and improving memory and strain ability; compared with large compound health food or prescriptions, the Chinese yam, hawthorn and salvia miltiorrhiza are low in cost, simple in preparation process and suitable for middle-aged and elderly people to discontinuously take for a long time, the cost is reduced, and the market competitiveness is improved; the composition has a remarkable anti-oxidation effect, can scavenge free radicals, delay senescence and prevent occurrence and development of various chronic diseases, shows a remarkable technical effect and a wide market application prospect in the field of anti-aging and anti-oxidation medicines, and provides an economic, safe and effective health guarantee scheme for old people.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition with anti-aging and anti-oxidation functions, and a preparation method and application thereof. Background Art

[0002] As people age, their physiological functions gradually decline, especially after the age of 40, when some organs begin to malfunction, and after the age of 60, the decline accelerates. This physiological change has led to an increasing demand for health care products among the middle-aged and elderly, who hope to delay the process of physical decline and thus prolong their lifespan by taking appropriate health care products.

[0003] Throughout the ages, Chinese medicine health care prescriptions or products for the purpose of prolonging life have emerged in an endless stream. Most of them are large compound prescriptions containing multiple tonic Chinese medicines. 1 to 2 courses of treatment may achieve certain health effects, but long-term use may lead to overcorrection, and most tonic Chinese medicines are expensive, making it difficult for users to take them for a long time, resulting in a lack of complete verification of whether they can really fight aging. For example, the Changchun Yishoudan recorded in "Cixi Guangxu Medical Prescription Selection" contains 23 Chinese medicines, most of which are tonic Chinese medicines. Taking them for more than 100 days can moisturize the face, strengthen teeth and black hair, but it is difficult for ordinary people to take them for a long time, so whether they can fight aging has been unknown for more than 100 years. A Chinese patent discloses a Chinese medicine composition for preventing diseases and its preparation method (publication number CN110025760A) involving 59 Chinese medicine raw materials and 4 extraction processes. The high cost of medicinal materials and preparation leads to high retail prices, which are difficult for ordinary people to afford, and there is no opportunity to participate in verifying its effects. Products with strong targeting but limited applicable population, such as yak bone strengthening powder, which is composed of bone and bone marrow, mainly supplement calcium to strengthen bones and muscles, and also improve immunity, but the applicable population is very limited and it is not suitable for long-term use. In view of the problems existing in the existing Chinese medicine health products, the applicant believes that the correct development direction includes: 1) mild effect, can be taken for a long time; 2) the formula should be relatively simple, avoid the use of too many medicinal materials, simple preparation process, low cost; 3) the applicable population is wide, which can meet the needs of different age groups and health conditions.

[0004] Chinese yam and hawthorn are both food and Chinese medicine; salvia miltiorrhiza is mainly used as medicine and can also be used as health food. According to the theory of Chinese medicine, Chinese yam is sweet in taste, neutral in nature, enters the spleen, lung and kidney meridians, and has the effects of nourishing the spleen and stomach, promoting fluid and benefiting the lungs, and nourishing the kidney and astringing the essence; hawthorn is sour and sweet in taste, slightly warm in nature, enters the spleen, stomach and liver meridians, and has the effects of strengthening the stomach and digestion, promoting blood circulation and resolving phlegm; salvia miltiorrhiza is bitter in taste, slightly cold in nature, enters the heart and pericardium meridians, and has the effects of promoting blood circulation and menstruation, cooling blood and eliminating carbuncle, removing blood stasis and relieving pain, and clearing the heart and calming the mind. Modern scientific research shows that yam contains polysaccharides, amino acids, fatty acids, yamin, allantoin, trace elements and other chemical components, which have the effects of lowering blood sugar, lowering blood lipids, anti-oxidation, anti-inflammatory, anti-tumor and enhancing immunity; hawthorn contains a variety of flavonoids, triterpenes and proteins, fats, organic acids, multiple vitamins and calcium, iron, phosphorus and other chemical components, which aid digestion, antibacterial, anti-cancer, antioxidant, enhance immunity, lower blood lipids, lower blood pressure and strengthen the heart, and can also improve osteoporosis; Salvia miltiorrhiza contains a variety of fat-soluble tanshinones, quinones and a variety of water-soluble salvianolic acid components, which have the effects of nourishing blood, lowering blood pressure, anti-thrombosis and anti-arrhythmia. Summary of the invention

[0005] In view of the problems and technical status quo of existing and previous anti-aging drugs or health foods, such as high cost of large compound prescriptions, single effect and limited applicable population, as well as difficulty and inappropriateness for long-term use, the present invention aims to provide a Chinese medicine composition with anti-aging and antioxidant functions and its preparation and use. By selecting medicinal materials with mild effects, long-term use, simple formulation and preparation process and low cost, and combining the pharmacological effects and chemical components of yam, hawthorn and salvia miltiorrhiza, a Chinese medicine composition with anti-aging and antioxidant functions suitable for a wide range of people is developed.

[0006] The present invention is achieved through the following technical solutions:

[0007] The invention provides a traditional Chinese medicine composition with anti-aging and anti-oxidation functions. The traditional Chinese medicine composition with anti-aging and anti-oxidation functions consists of 450-550 parts of yam, 300-350 parts of hawthorn and 180-200 parts of salvia miltiorrhiza according to weight.

[0008] The yam is Chinese yam, the hawthorn is charred hawthorn, and the salvia miltiorrhiza is vinegar-processed salvia miltiorrhiza.

[0009] Furthermore, in parts by weight, the Chinese medicinal composition with anti-aging and antioxidant functions consists of 500 parts of yam, 310 parts of hawthorn and 190 parts of salvia miltiorrhiza.

[0010] The present invention provides a method for preparing the above-mentioned Chinese medicine composition with anti-aging and antioxidant functions, comprising: drying yam, hawthorn and salvia miltiorrhiza, crushing and sieving them respectively, and mixing them to obtain the Chinese medicine composition with anti-aging and antioxidant functions.

[0011] Furthermore, the seeds of the hawthorn are removed before drying.

[0012] The drying temperature is 40° C. to 50° C., and the drying time is 10 h to 16 h.

[0013] The mesh number of the sieving screen is 80 to 120 meshes.

[0014] Application of the above-mentioned Chinese medicine composition with anti-aging and antioxidant functions in the preparation of anti-aging drugs or health foods.

[0015] Application of the above-mentioned Chinese medicine composition with anti-aging and anti-oxidation functions in the preparation of antioxidant drugs or health foods.

[0016] The present invention provides a medicine with anti-aging and anti-oxidation functions, which is prepared from the above-mentioned traditional Chinese medicine composition with anti-aging and anti-oxidation functions and / or pharmaceutically acceptable excipients.

[0017] The dosage form of the medicine is any one of powder, capsule, granule, tablet and pill.

[0018] The use of the Chinese medicine composition with anti-aging and antioxidant functions of the present invention is suitable for middle-aged and elderly men and women over 40 years old, and can be used intermittently for a long time, that is, use it continuously for 1 week and then stop using it for 2 weeks. Take 3 to 6 grams a day, which can be taken once or divided into several times, and can be taken alone before or after meals, or taken with food (including mixing it into food).

[0019] Compared with the existing anti-aging Chinese medicine compositions and products thereof, the present invention has the following beneficial effects:

[0020] The Chinese medicine composition with anti-aging and anti-oxidation functions provided by the present invention has the advantages of multi-pathway action, low cost, significant anti-oxidation effect, improvement of physiological functions of the elderly, improvement of anti-stress ability, and reliable effect verified by animal experiments. The Chinese medicine composition produces anti-aging and anti-oxidation effects through multiple pathways, including improving elderly digestive function, vitamin and trace element metabolism, anti-oxidation ability, aging-related protein expression, anti-stress ability, and memory and resilience. This multi-pathway mode of action can improve the physiological and metabolic state of the elderly more comprehensively, thereby achieving a better comprehensive effect; compared with health foods or prescriptions with high costs for large compound prescriptions, the Chinese medicine composition uses low-cost medicinal materials and is suitable for long-term intermittent use by middle-aged and elderly people, which reduces the economic burden of consumers, improves the market competitiveness of products, and is conducive to the promotion and popularization of products; the Chinese medicine composition has a significant anti-oxidation effect, can remove free radicals, reduce the generation of peroxides, thereby delaying the aging process and the occurrence and development of various chronic diseases. Antioxidation is one of the key mechanisms of anti-aging, so the composition has significant advantages in anti-aging and anti-oxidation; by improving the digestive function, vitamin and trace element metabolism, and aging-related protein expression of the elderly, the Chinese medicine composition can significantly improve the physiological function and quality of life of the elderly. At the same time, improving the elderly's memory and ability to cope with emergencies is also conducive to improving the survival ability of the elderly; the Chinese medicine composition can improve the elderly's ability to resist stress under extremely adverse conditions and increase their chances of survival under these conditions, which is of great significance for the elderly to cope with emergencies and maintain good health.

[0021] The preparation method provided by the present invention uses yam, hawthorn and salvia miltiorrhiza as raw materials of the traditional Chinese medicine composition, which has potential anti-aging and anti-oxidation effects; the relatively mild temperature range helps to retain the effective ingredients in the raw materials and avoid the loss or destruction of the ingredients caused by high temperature.

[0022] The application provided by the present invention, the application of the Chinese medicine composition of the present invention in the preparation of anti-aging drugs or health foods and antioxidant drugs or health foods has significant technical effects and broad market prospects. It not only helps to improve the physiological and metabolic state of the elderly and improve the quality of life, but also prevents the occurrence and development of various chronic diseases, providing a safe, effective and economical health protection plan for the elderly. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a graph showing the effect of the Chinese medicine composition of the present invention on the expression of Klotho protein in the kidney of aged mice. Compared with the young mice group, **P<0.01; compared with the aged mice saline group, #P<0.05, ##P<0.01. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the scheme of the present invention, the technical scheme in the embodiment of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiment of the present invention. Obviously, the described embodiment is only an embodiment of a part of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work should fall within the scope of protection of the present invention.

[0025] The present invention is further described in detail below in conjunction with the accompanying drawings:

[0026] If no specific experimental steps or conditions are specified in the examples, the experiments can be carried out according to the conventional experimental steps or conditions described in the literature in the art. If no manufacturer is specified for the reagents or instruments used, they are all conventional reagent products that can be obtained commercially.

[0027] Example 1

[0028] This embodiment provides a Chinese medicine composition with anti-aging and antioxidant functions, and the specific preparation process is as follows:

[0029] 500g of Chinese yam, 310g of charred hawthorn (without seeds), and 190g of vinegar-processed salvia miltiorrhiza were respectively placed in an oven at 50°C overnight and then crushed and sieved. The sieve aperture was 0.15 mm, i.e., 100 mesh / inch. The fine powder passing through the sieve was collected, the three fine powders were mixed, and packaged and sealed into bagged powders (6 g / bag).

[0030] Example 2

[0031] 450g of yam, 300g of hawthorn and 180g of salvia miltiorrhiza fine powder were mixed, and the mixture was packaged and sealed into bagged powders (6g / bag) to obtain a traditional Chinese medicine composition with anti-aging and antioxidant functions.

[0032] Example 3

[0033] 550 g of Chinese yam, 350 g of hawthorn and 200 g of salvia miltiorrhiza powder were mixed, and the mixture was packaged and sealed into bagged powders (6 g / bag) to obtain a traditional Chinese medicine composition with anti-aging and antioxidant functions.

[0034] Example 4

[0035] Based on Examples 1 to 3, this example conducts relevant performance verification tests on the Chinese medicine composition with anti-aging and antioxidant functions obtained by the present invention.

[0036] 1. Experiment on the effect of Chinese herbal medicine composition with anti-aging and antioxidant functions on animal lifespan

[0037] Animals: 80 Kunming mice with clear birth dates (half male and half female, supplied by the Experimental Animal Center of Xi'an Jiaotong University) were ordered. After 20 days of age, breastfeeding was stopped and the mice were randomly divided into 4 groups with balanced kinship and gender among the groups. Male and female mice in each group were housed in separate cages.

[0038] Dosage scheme: blank control group: mice were given standard mouse feed throughout their lives as the baseline of the experiment, experimental group 1: mice were given mouse feed containing 0.4% of the Chinese medicine composition with anti-aging and antioxidant functions of Example 1 of the present invention at 300 days of age (10 months of age, equivalent to 40 years of age); experimental group 2: mice were given mouse feed containing 0.4% of the Chinese medicine composition with anti-aging and antioxidant functions of Example 1 of the present invention at 455 days of age (15 months of age, equivalent to 60 years of age), experimental group 3: mice were given mouse feed containing 0.4% of the Chinese medicine composition with anti-aging and antioxidant functions for 1 week + standard mouse feed for 2 weeks in alternating mode at 455 days of age. All mice were given free water, animals were checked daily, and the life span of dead animals was recorded. The specific results are shown in Table 1.

[0039] From the data in Table 1, we can see that the average lifespan of mice in Experimental Group 3 was the longest, which was about 70 days longer than the average lifespan of mice in the blank control group (equivalent to 9.3 years for humans); in addition, the shortest and longest lifespans of mice in Experimental Group 3 were also longer than those in the blank control group, and the longest-lived mice in Experimental Group 3 lived 119 days longer than the longest-lived mice in the blank control group, which is equivalent to about 16 years for humans. The shortest-lived mice in Experimental Group 1 died of tumors, and the cause of death of the shortest-lived mice in other groups was unknown. The longest-lived mice in each group did not die of tumors, and some other mice in each group died of tumors (1 to 2 cases in each group), but the cause of death of most of them was unknown.

[0040] Table 1: Effects of different administration regimens of Chinese medicine composition on the life span of mice (days)

[0041]

[0042] In this experiment, mice aged 300 and 455 days were set as the starting age for taking the Chinese medicine composition with anti-aging and antioxidant functions. Mice that died before 300 days of age were removed from their groups. Two modes of continuous and intermittent administration of the Chinese medicine composition were set. Three experimental groups were designed with comprehensive consideration of the above factors. The average weight of middle-aged and elderly mice was 40g, and the average daily effective food intake was about 4g. Giving feed containing 0.4% of the Chinese medicine composition was equivalent to a daily intake of 0.4g / kg of the Chinese medicine composition.

[0043] Effectiveness of long-term intermittent administration: The average lifespan of mice in Experimental 3 was extended, while that in Experimental Groups 1 and 2 was not extended, indicating that long-term intermittent administration of the Chinese medicine composition is effective, while continuous administration may produce an "overcorrection" effect, offsetting the beneficial effects; Mechanism of intermittent administration: Continuous administration for one week can ensure a health-care effect of a certain intensity and duration, and stopping administration for two weeks can allow the body to eliminate the residual Chinese medicine composition in the body, avoid the accumulation of drug components, and restore the body's sensitivity to the Chinese medicine composition; Estimation of the starting time of administration: Based on the experimental results, the life-extending effect of long-term intermittent administration of the Chinese medicine composition starting at 300 days of age should be no less than that starting at 455 days of age.

[0044] In summary, mice can prolong their lifespan by intermittently taking Chinese medicine compositions with anti-aging and antioxidant functions since middle age or old age. According to the species differences, surface area / body weight ratio, and activity level of humans and mice, it is recommended that people start taking Chinese medicine compositions with anti-aging and antioxidant functions intermittently for a long time from the age of 40 to 60 years old, and take 3 to 6 g per day during the period of taking them. They can be taken once or in divided doses, and can be taken alone or with food. This experiment verified that the Chinese medicine compositions with anti-aging and antioxidant functions obtained by the present invention have anti-aging and antioxidant effects through animal experiments, and explored the best mode of taking and starting age. The experimental results have important reference value for guiding humans to reasonably take Chinese medicine compositions with anti-aging and antioxidant functions.

[0045] 2. Animal acute toxicity test of Chinese herbal medicine composition with anti-aging and antioxidant functions

[0046] This study aims to evaluate the acute toxicity of a Chinese herbal composition with anti-aging and antioxidant functions in mice, or to determine its maximum tolerated dose (MTD), thereby providing a basis for inferring its safety for human use.

[0047] Experimental animals: Kunming mice were selected, 9 males (preliminary test) and 20 females (half male and half female) (formal test), with body weight between 18-20g;

[0048] Drug preparation: The suspension of the Chinese medicine composition with anti-aging and antioxidant functions (Example 1 of the present invention) was prepared using distilled water, and the highest concentration that did not block the intragastric needle was 12%.

[0049] Dosage:

[0050] (1) Preliminary test

[0051] Nine mice were randomly divided into three groups. After fasting for 8 hours, they were gavaged with 12%, 6% and 3% suspension of the Chinese medicine composition with anti-aging and antioxidant functions, respectively. The gavage volume was 0.4 mL / 10 g mouse body weight, and they were observed for 72 hours.

[0052] (2) Formal test

[0053] 20 mice were first kept in the animal room for 1 week to adapt to the environment. After fasting for 8 hours, they were given 12% concentration of Chinese medicine composition suspension with anti-aging and antioxidant functions by gavage for 3 times in a row, 4 hours apart each time, and the gavage volume was 0.4mL / 10g body weight; after the second and third gavages, the feces of all animals were thin and mushy, and other conditions were normal. The next day, the feces of all animals returned to normal. After 7 consecutive days of observation, no animals died, and there were no other physiological abnormalities.

[0054] Preliminary test results: All mice were observed for 72 hours without death, moved freely, and had no other physiological abnormalities, including the appearance of feces. Considering that 12% is the maximum concentration of the suspension of the Chinese medicine composition with anti-aging and antioxidant functions that can be smoothly administered to mice by gavage, 0.4mL / 10g is the maximum safe volume for gavage administration to mice, and no animals died, it is estimated that the LD 50 , the formal trial was adjusted to maximum tolerated dose observation.

[0055] Formal test results: When mice were gavaged with a Chinese medicine composition with anti-aging and antioxidant functions at the highest concentration (12%) and the largest volume (1.2 ml / 10 g) within one day, no acute toxicity was observed for 7 consecutive days. It is believed that the mice can tolerate a daily dose of 14.4 g / kg, which is equivalent to 168 to 336 times the human daily dose of 0.043 g / kg (daily dose 3 to 6 g, based on a body weight of 70 kg).

[0056] In summary, according to the results of this test, people will not suffer from acute poisoning if they take 3-6g of the Chinese medicine composition with anti-aging and antioxidant functions daily. This conclusion provides a basis for the safety of the practical application of the Chinese medicine composition with anti-aging and antioxidant functions.

[0057] 3. Effects of a Chinese herbal medicine composition with anti-aging and antioxidant functions on gastric emptying, vitamin C, vitamin E, selenium, antioxidant activity, and Klotho protein expression in aged mice

[0058] Animals: Aged (over 15 months old) female Kunming mice were eliminated production mice from the Experimental Animal Center of Xi'an Jiaotong University, and young mice were 8-week-old female mice of the same strain.

[0059] Method: Aged mice were randomly divided into groups and given Chinese medicine composition (Example 1 of the present invention) or yam powder, hawthorn powder, salvia powder, metoclopramide (Beijing Dayang Pharmaceutical Company, tablets, product batch number 532293, ground into powder and prepared into suspension of required concentration with normal saline) or normal saline by oral gavage at a volume of 0.2 mL / 10 g, once a day for 7 consecutive days. On the 7th day, the mice were fasted but not watered for 8 hours before oral gavage. One hour after oral gavage, the mice were then gavaged with 8 mL / mouse of homemade nutritious semi-solid food paste. After 20 minutes, the animals were killed by cervical dislocation, and the heart was opened to draw blood; the liver sample and one kidney were cut by abdominal opening, the gastric cardia and pylorus were ligated, the stomach was taken and weighed, and then the stomach was cut open along the greater curvature of the stomach to wash out the contents and weigh the net weight of the stomach, and the gastric residual rate (%) reflecting the gastric emptying of the mice was calculated = (total stomach weight - net weight) ÷ 8 mL food paste weight × 100%; the brain tissue sample was taken by craniotomy. Serum was separated to measure the contents of vitamin C (spectrophotometry), vitamin E (gas chromatography), selenium (high performance liquid chromatography), malondialdehyde (thiobarbituric acid colorimetry), superoxide dismutase (kit method), and glutathione peroxidase (kit method). Liver and brain tissue sample homogenates were used to measure the contents of malondialdehyde, superoxide dismutase, and glutathione peroxidase. Kidney sample homogenates were used to measure the expression of Klotho protein (immunoblotting).

[0060] (3) Results

[0061] The effects of the Chinese herbal composition with anti-aging and antioxidant functions and its components, namely, yam powder, hawthorn powder and salvia powder, on gastric emptying of aged mice are shown in Table 2, the effects on serum vitamin C, vitamin E and selenium concentrations are shown in Table 3, the effects on malondialdehyde content and superoxide dismutase and glutathione peroxidase activities in serum, liver and brain are shown in Table 4, Table 5 and Table 6, respectively, and the effects on Klotho protein expression in renal cortex are shown in the attached figure Figure 1 shown.

[0062] Table 2: Effects of the Chinese herbal medicine composition with anti-aging and antioxidant functions on gastric emptying in aged mice

[0063]

[0064] Compared with the young rats saline group, △P<0.05; compared with the old rats saline group, *P<0.05, **P<0.01.

[0065] As shown in Table 2, gastric emptying in old mice was weaker than that in young mice. Taking large doses of hawthorn and salvia miltiorrhiza for one week can improve gastric emptying in old mice, but small doses are not obvious. Using yam alone does not affect gastric emptying. The Chinese medicine composition with anti-aging and antioxidant functions can significantly improve gastric emptying at a small dose, indicating that the combination of hawthorn, salvia miltiorrhiza and yam has an additive or synergistic digestive effect.

[0066] Table 3: Effects of Chinese herbal medicine composition on serum vitamin C (mg / dL), E (mg / dL) and selenium (μg / L) in aged mice

[0067]

[0068] Compared with the saline group of young mice, △P<0.05, △△P<0.01; compared with the saline group of old mice, *P<0.05, **P<0.01.

[0069] Table 4: Effect of Chinese medicine combination on malondialdehyde content in serum, liver and brain tissue of aged mice

[0070]

[0071]

[0072] Compared with the saline group of young mice, △P<0.05, △△P<0.01; compared with the saline group of old mice, *P<0.05, **P<0.01.

[0073] Table 5: Effects of Chinese herbal medicine combination on superoxide dismutase activity in serum, liver and brain tissues of aged mice

[0074]

[0075] Compared with the saline group of young mice, △P<0.05, △△P<0.01; compared with the saline group of old mice, *P<0.05, **P<0.01.

[0076] Table 6: Effects of Chinese herbal medicine combination on glutathione peroxidase activity in serum, liver and brain of aged mice

[0077]

[0078] Compared with the saline group of elderly rats, *P<0.05, **P<0.01.

[0079] 4. Discussion and Conclusion

[0080] This experiment observed the effects of a traditional Chinese medicine composition with anti-aging and antioxidant functions and its components, yam, hawthorn, and salvia miltiorrhiza, on gastric emptying in aged mice, with metoclopramide as a positive control drug; at the same time, the effects of the traditional Chinese medicine composition on the antioxidant capacity of aged mice and the expression of aging-related Klotho protein were investigated.

[0081] As shown in Table 3, the Chinese medicine composition with anti-aging and antioxidant functions and its components do not affect the serum vitamin C concentration of elderly mice when taken continuously. The Chinese medicine composition can significantly improve the decline of serum vitamin E concentration in elderly mice when used alone, indicating that the Chinese medicine composition does not directly supplement vitamin E, but its components, Chinese medicine, hawthorn and salvia miltiorrhiza, indirectly improve blood vitamin E through the synergistic or additive effects of other effects. Hawthorn and salvia miltiorrhiza do not affect the blood selenium concentration of elderly mice when used alone, and a large dose of Chinese medicine can significantly improve the decline of blood selenium in elderly mice, while a small dose of Chinese medicine composition can significantly improve it, indicating that Chinese medicine can directly or indirectly improve the decline of blood selenium in elderly mice, and the combined use of hawthorn and salvia miltiorrhiza can enhance the effect.

[0082] As shown in Tables 4, 5 and 6, the antioxidant capacity of aged mice decreased, which was manifested by increased malondialdehyde content in blood, liver and brain, and decreased activities of superoxide dismutase and glutathione peroxidase. The Chinese medicine composition with anti-aging and antioxidant functions of the present invention can significantly improve the antioxidant capacity in aged mice, and has a stronger effect than that of yam, hawthorn and salvia miltiorrhiza used alone.

[0083] The Klotho gene is involved in regulating phosphate homeostasis and fibroblast growth factor signaling. The expression level of Klotho protein decreases with age and is directly related to aging. Therefore, regulating Klotho activity is an important entry point for anti-aging and longevity. Figure 1 As shown, Danshen does not affect the expression of Klotho protein in aged mice, while Chinese yam and hawthorn can improve the expression of Klotho protein in aged mice when used alone. The Chinese medicine composition with anti-aging and antioxidant functions of the present invention has a stronger effect than Chinese yam and hawthorn when used alone.

[0084] Summary: The anti-aging and antioxidant effects of the Chinese herbal composition of the present invention are partly attributed to its improvement of the digestive function (gastric emptying) and antioxidant capacity in the body and Klotho protein expression in elderly mice. Its improved antioxidant capacity may be partly related to the improvement of blood vitamin E and selenium concentrations.

[0085] Example 5

[0086] This example, based on Examples 1 to 3, investigates the effect of the Chinese medicinal composition with anti-aging and antioxidant functions obtained by the present invention on the anti-stress ability of elderly mice.

[0087] Animals: Aged (over 15 months old) Kunming strain (KM) female mice were eliminated from the Experimental Animal Center of Xi'an Jiaotong University, and young mice were 8-week-old female mice of the same strain.

[0088] Method: The Chinese medicine composition with anti-aging and antioxidant functions of the present invention or the decoction of red ginseng or distilled water was continuously gavaged for 7 days, once a day, and the test was started 30 minutes after the last gavage. Hypoxia tolerance experiment: Each mouse was placed in a wide-mouthed glass bottle with a capacity of 250 mL and 15 g of soda lime, and the bottle mouth was coated with vaseline and the bottle cap was tightly closed. The room temperature was 18°C. The survival time of the mouse in the bottle was the hypoxia tolerance time. Low temperature resistance experiment: The mice were placed in a refrigerator, in the same group and in the same box (cage), and the temperature in the box was -10°C. After 30 minutes, the refrigerator was opened and checked every 15 minutes. The surviving mice were considered to be resistant to low temperatures until this time period. High temperature resistance experiment: The mice were placed in a 45°C constant temperature drying box, in the same group and in the same box (cage), and the death time of each mouse was recorded. The surviving mice were considered to be resistant to high temperatures until this time period.

[0089] The effects of the Chinese medicinal composition with anti-aging and anti-oxidation functions of the present invention on the hypoxia tolerance time, low temperature tolerance time and high temperature tolerance time of elderly mice are shown in Table 7, Table 8 and Table 9, respectively.

[0090] Table 7: Effect of Chinese medicine composition on normal pressure hypoxia tolerance time of elderly mice (all n = 6)

[0091]

[0092] a refers to the comparison with the distilled water group of young mice, and b refers to the comparison with the distilled water group of old mice.

[0093] Table 8: Effect of Chinese medicine composition on low temperature tolerance time of elderly mice (all n = 10)

[0094]

[0095] a refers to the comparison with the distilled water group of young mice, and b refers to the comparison with the distilled water group of old mice.

[0096] Table 9: Effect of Chinese medicine composition on high temperature tolerance time of elderly mice (all n = 10)

[0097]

[0098]

[0099] a refers to the comparison with the distilled water group of young mice, and b refers to the comparison with the distilled water group of old mice.

[0100] 4. Discussion and Conclusion

[0101] This experiment observed the effect of a Chinese herbal composition with anti-aging and antioxidant functions on the anti-stress ability of elderly mice, and red ginseng decoction was used as a positive control drug. The concentration of red ginseng decoction of 12.5% ​​means that 100 mL contains the equivalent of 12.5 g of red ginseng decoction extract: red ginseng slices were soaked for 20 minutes, the first decoction was 30 minutes, the second decoction was 15 minutes, and the two decoctions were combined and concentrated to 100 mL.

[0102] As shown in Tables 7, 8 and 9, the hypoxia tolerance time, low temperature tolerance time and high temperature tolerance time of old mice were shorter than those of young mice. The Chinese herbal composition with anti-aging and antioxidant functions can prolong the hypoxia tolerance time, low temperature tolerance time and high temperature tolerance time of old mice, that is, improve the anti-stress ability of old mice, increase their chances of survival under extreme conditions, and buy a certain amount of time for them to escape from difficulties or be rescued.

[0103] Example 6

[0104] The effect of the Chinese medicinal composition with anti-aging and antioxidant functions of the present invention on the memory of aged mice was investigated. Animals: aged mice were female Kunming mice over 15 months old; young mice were female mice of 8 weeks old.

[0105] Methods: Electrical stimulation jumping platform method: Mice were continuously gavaged with drugs or distilled water once a day for 7 days. On the 5th and 6th days, electrical stimulation-jumping platform training was performed 1 hour after drug administration. On the 7th day, 1 hour after drug administration, the mice were placed on the platform to test the time of the first jump off the platform (electric shock latency) and the number of times they jumped off the platform and were electrocuted (both feet touched the electrified copper grid at the same time) within 5 minutes.

[0106] Results: The effects of the Chinese medicinal composition with anti-aging and antioxidant functions of the present invention on the electric shock latency and the number of electric shocks within 5 minutes of elderly mice are shown in Table 10.

[0107] Table 10: Effects of the Chinese medicine composition on the electric shock latency and the number of electric shocks within 5 minutes in elderly mice (both n=10)

[0108]

[0109]

[0110] Compared with the young rat saline group, △△P<0.01; compared with the old rat saline group, *P<0.05, **P<0.01.

[0111] This experiment uses the electrical stimulation-jumping platform method to evaluate the effect of a Chinese medicine composition with anti-aging and antioxidant functions on the memory of elderly mice. After 2 days of electrical stimulation-jumping platform training, mice acquire the memory that the electrified copper grid is harmful. When tested, they are placed on the jumping platform and the time of the first jump off the platform, i.e., the electric shock latency, is recorded, which can reflect the memory ability of harmful stimulation. However, during the jump, the memory that the electrified copper grid is harmful may suddenly be restored, so that one foot touches the copper grid to avoid electric shock. Therefore, the number of electric shocks (jumping off the platform and both feet touching the copper grid at the same time) within 5 minutes reflects not only the memory ability but also the ability to respond to emergencies (intelligence). In this experiment, Naofukang (Shandong Renhetang Pharmaceutical Co., Ltd., tablets, product batch number 231144, ground into powder and prepared into a suspension of the required concentration with physiological saline) is used as the positive control drug.

[0112] As shown in Table 10, the latency of electric shock in old mice was shorter than that in young mice, and the number of electric shocks within 5 minutes increased. The Chinese medicine composition with anti-aging and antioxidant functions of the present invention can prolong the latency of electric shock in old mice and reduce the number of electric shocks within 5 minutes, indicating that the memory and emergency response ability of old mice are improved, which helps to reduce damage and improve survival ability.

[0113] The above contents are only for explaining the technical idea of ​​the present invention and cannot be used to limit the protection scope of the present invention. Any changes made on the basis of the technical solution in accordance with the technical idea proposed by the present invention shall fall within the protection scope of the claims of the present invention.

Claims

1. A Chinese medicine composition with anti-aging and antioxidant functions, characterized in that: The traditional Chinese medicine composition with anti-aging and anti-oxidation functions consists of 450-550 parts of yam, 300-350 parts of hawthorn and 180-200 parts of salvia miltiorrhiza, according to parts by weight.

2. The Chinese medicine composition with anti-aging and antioxidant functions according to claim 1, characterized in that: The yam is Chinese yam, the hawthorn is charred hawthorn, and the salvia miltiorrhiza is vinegar-processed salvia miltiorrhiza.

3. The Chinese medicine composition with anti-aging and antioxidant functions according to claim 1, characterized in that: According to weight, the Chinese medicinal composition with anti-aging and antioxidant functions consists of 500 parts of yam, 310 parts of hawthorn and 190 parts of salvia miltiorrhiza.

4. The method for preparing the Chinese medicine composition with anti-aging and antioxidant functions according to any one of claims 1 to 3, characterized in that: include: Chinese yam, hawthorn and salvia miltiorrhiza are dried, crushed and sieved respectively, and mixed to obtain a traditional Chinese medicine composition with anti-aging and anti-oxidation functions.

5. The method for preparing the Chinese medicine composition with anti-aging and antioxidant functions according to claim 4, characterized in that: The drying temperature is 40° C. to 50° C., and the drying time is 10 h to 16 h.

6. The method for preparing the Chinese medicine composition with anti-aging and antioxidant functions according to claim 4, characterized in that: The mesh number of the sieving screen is 80 to 120 meshes.

7. Use of the traditional Chinese medicine composition with anti-aging and antioxidant functions according to any one of claims 1 to 3 in the preparation of anti-aging drugs or health foods.

8. Use of the traditional Chinese medicine composition with anti-aging and antioxidant functions according to any one of claims 1 to 3 in the preparation of antioxidant drugs or health foods.

9. A drug with anti-aging and anti-oxidation functions, characterized in that: The medicine is prepared from the Chinese medicine composition with anti-aging and antioxidant functions as claimed in any one of claims 1 to 3 and / or pharmaceutically acceptable excipients.

10. The drug with anti-aging and anti-oxidation functions according to claim 9, characterized in that: The dosage form of the medicine is any one of powder, capsule, granule, tablet and pill.

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