Preparation method of inflammation-promoting repair mask and product thereof
The hydrogel mask is formed by electrospinning technology by loading simulated enzymes and combining reactive oxygen crosslinking agents, which solves the shortcomings of traditional wound dressings in controlling wound ROS overdose and collaborative repair of the skin, achieving efficient antibacterial and wound repair effects.
Patent Information
- Application Number
- CN202411893649.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-20
- Publication Date
- 2025-03-25
AI Technical Summary
Traditional wound dressings have shortcomings in promoting skin inflammation repair, especially in controlling wound ROS overdose and synergistic repair of the skin.
The fiber base film was prepared by electrospinning technology and the reactive oxygen crosslinking agent TSPBA was used to integrate PVA and Chinese medicine extract with the substrate to form a hydrogel repair mask. The phenylborate bond was broken by releasing antibacterial Chinese medicine ingredients, and the wound was removed from excessive ROS.
The antibacterial function of hydrogel mask and the removal of excessive ROS in wound inflammatory wounds is achieved, and the skin is repaired in collaboration, which significantly improves the proliferation vitality of fibroblasts.
Smart Images
Figure FT_1
Abstract
Description
Technical Field
[0001] The present invention relates to a preparation method of a pro-inflammatory repairing facial mask and a product thereof, and in particular to a hydrogel facial mask based on electrospinning and microenvironment ROS response. The present invention belongs to the field of nano-biomedical materials. Background Art
[0002] The skin is the largest organ in the human body. Healthy skin can serve as a protective barrier, blocking the acute inflammatory response of the skin caused by ultraviolet rays, bacteria and other substances. Traditionally, skin damage repair is divided into four stages: (1) hemostasis; (2) inflammation; (3) proliferation; and (4) maturation and remodeling. The four stages can overlap. That is, after skin damage, the wound first initiates the hemostasis process, and platelets immediately begin to aggregate at the site of damage, releasing procoagulant factors, chemokines and growth factors; subsequently, immune cells such as neutrophils, macrophages and lymphocytes chemotaxis to the wound surface and secrete proinflammatory cytokines to promote the wound healing process. However, studies have found that ROS appear on the wound surface before inflammatory cells, and induce inflammatory cells to the wound surface through chemotaxis and initiate wound repair. When the skin is damaged, the increase in ROS on the wound surface mainly comes from the continuous generation mediated by NADPH oxidase in cells such as fibroblasts, keratinocytes, and endothelial cells; and the short-term and large-scale generation of phagocyte respiratory burst. After skin injury, a large amount of ROS is produced on the wound surface, inducing the production of a large number of inflammatory mediators, oxidizing lipids and proteins on the cell membrane, damaging DNA in the nucleus and mitochondria, and causing oxidative stress, which has an adverse effect on wound repair.
[0003] Anti-inflammation is a key and complex physiological response process in the wound healing process. Currently, most wound dressings mainly provide a protective and moisturizing layer to prevent cross-infection, while anti-inflammation is usually induced by the additional addition of other bioactive molecules. Hydrogel dressings are a new type of material used for skin wounds. They have great application potential in wound dressings because of their ability to absorb exudate well, maintain the wound in a moist environment, not easily adhere to injured tissues, and achieve sustained drug release. Summary of the invention
[0004] The present invention aims to provide a method for preparing an inflammation-promoting repairing facial mask.
[0005] Another object of the present invention is to provide a pro-inflammatory repair facial mask product prepared by the above method.
[0006] The purpose of the present invention is achieved through the following scheme: a method for preparing a pro-inflammatory repair mask, firstly preparing a fiber basement membrane by electrospinning loaded with simulated enzyme, then placing the basement membrane into a solution containing a Chinese medicine extract, and using an active oxygen crosslinking agent N1-(4-bromobenzyl)-N3-(4-bromophenyl)-N1,N1,N3,N3-tetramethylpropane-1,3-diamine (TSPBA) to integrate polyvinyl alcohol (PVA), the Chinese medicine extract and the basement to form a hydrogel repair mask, comprising the following steps: (1) Preparation of fiber spinning membrane: Dissolve the simulated enzyme in a silk fibroin (SF) solution with a concentration of 20% to 60% by mass, mix evenly at 60°C to obtain a concentrated spinning solution; load the spinning solution into a 10 mL syringe, set the electrospinning pressure to 15 to 20 kV, and perform electrospinning at a flow rate of 0.005 to 0.02 mL / h within a receiving distance of 12 to 20 cm to obtain a 0.05 to 0.2 mm thick fiber membrane. The above fiber membrane is vacuum dried overnight for use; (2) Preparation of hydrogel mask: Place the fiber membrane in a solution containing Chinese herbal extract, add 2% to 10% PVA by mass and 0.1% to 5% TSPBA by mass, stir thoroughly, and let stand to obtain a hydrogel mask.
[0007] The mimetic enzyme of the present invention has the ability to scavenge free radicals and can be any one of iron-based nanozymes, nanogold, cerium-based metal organic framework materials, manganese-based metal organic framework materials, and the like.
[0008] The size of the nano-gold described in the present invention is 2-10 nm.
[0009] The added amount of the mimetic enzyme of the present invention is 0.1-0.8 g.
[0010] The main efficacy of the Chinese herbal extract of the present invention is antibacterial and anti-inflammatory, and the extract may be any one or more of coptis chinensis, lithospermum officinale, angelica dahurica, phellodendron amurense, isatis indigotica, honeysuckle and the like.
[0011] The present invention provides an inflammation-promoting repairing facial mask, characterized in that it is prepared according to any of the above-mentioned methods, and is a hydrogel facial mask based on electrospinning and microenvironment ROS response.
[0012] In the present invention, electrostatic spinning is used to prepare a fiber membrane substrate loaded with a simulated enzyme, and then based on the fiber membrane substrate, PVA is catalyzed to continue to cross-link on the fiber membrane to form a hydrogel membrane, and the traditional Chinese medicine components are loaded into the three-dimensional grid structure of the hydrogel; the cross-linking agent TSPBA used in the present invention contains a phenyl borate ester bond, and the ester bond breaks after encountering ROS, releasing the antibacterial traditional Chinese medicine components; at the same time, the simulated enzyme loaded on the fiber membrane substrate has the ability to scavenge free radicals, so the prepared hydrogel has the function of antibacterial and clearing excessive ROS in inflammatory wounds, thereby achieving the effect of synergistically repairing the skin.
[0013] The advantages of the present invention are: (1) Electrospinning was used to prepare a fiber membrane substrate loaded with a simulated enzyme, and then based on the fiber membrane substrate, PVA was catalyzed to continue cross-linking on the fiber membrane to form a hydrogel membrane, and the traditional Chinese medicine components were loaded into the three-dimensional grid structure of the hydrogel; (2) The cross-linking agent TSPBA used contains a phenyl borate ester bond. When it encounters ROS, the ester bond breaks and releases the antibacterial Chinese medicine ingredients. At the same time, the simulated enzyme loaded on the fiber membrane substrate has the ability to scavenge free radicals. Therefore, the prepared hydrogel has the function of antibacterial and clearing excess ROS in inflammatory wounds, achieving the effect of synergistically repairing the skin. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Attached Figure 1 Cell activity analysis of the hydrogels prepared in Examples 1-3. DETAILED DESCRIPTION
[0015] The technical scheme of the present invention is further described below through specific examples. The following examples are further explanations of the present invention, but do not limit the scope of the present invention.
[0016] Example 1 A pro-inflammatory repair mask is first prepared by electrospinning a simulated enzyme to prepare a fiber basement membrane, then the basement membrane is immersed in a solution containing a Chinese herbal medicine extract, and a reactive oxygen crosslinking agent N1-(4-bromobenzyl)-N3-(4-bromophenyl)-N1,N1,N3,N3-tetramethylpropane-1,3-diamine (TSPBA) is used to integrate polyvinyl alcohol (PVA), the Chinese herbal medicine extract and the basement to form a hydrogel repair mask, and the preparation is performed according to the following steps: (1) Preparation of fiber spinning membrane: 0.2 g of 5 nm enzyme-mimicking gold nanoparticles was dissolved in 30% by mass silk fibroin (SF) solution and mixed at 60 °C to obtain a concentrated spinning solution. The spinning solution was loaded into a 10 mL syringe, and the electrospinning pressure was set to 15-20 kV. The flow rate was 0.005-0.02 mL / h, and electrospinning was performed within a receiving distance of 12-20 cm to obtain a 0.2 mm thick fiber membrane. The obtained fiber membrane was vacuum dried overnight and set aside. (2) Preparation of hydrogel mask: Place the fiber membrane in a solution containing Coptis chinensis extract, add 3% by mass PVA and 0.5% by mass TSPBA solution, stir thoroughly, and let stand to obtain a hydrogel mask.
[0017] The prepared hydrogel mask promotes the proliferation activity of fibroblasts as shown in the figure Figure 1 As shown, the cell viability was 95%.
[0018] Example 2 A pro-inflammatory repair mask is prepared by the following steps, similar to the steps in the embodiment: (1) Preparation of fiber spinning membrane: 0.2 g of iron-based metal organic framework material was dissolved in 40% silk fibroin (SF) solution, mixed evenly at 60°C to obtain concentrated spinning solution; the spinning solution was loaded into a 10 mL syringe, the electrospinning pressure was set to 15-20 kV, and the flow rate was 0.005-0.02 mL / h. Electrospinning was performed within a receiving distance of 12-20 cm to obtain a 0.2 mm thick fiber membrane. The above fiber membrane was vacuum dried overnight for use; (2) Preparation of hydrogel mask: Place the fiber membrane into the Coptis chinensis extract solution, add 5% by mass PVA and 0.6% by mass TSPBA solution, stir thoroughly, and let stand to obtain a hydrogel mask.
[0019] The cell viability of the hydrogel mask obtained was 90%. Figure 1 shown.
[0020] Example 3 A pro-inflammatory repair mask is prepared by the following steps, similar to the steps in the embodiment: (1) Preparation of fiber spinning membrane: 0.3 g of Mn-based metal organic framework material was dissolved in 40% silk fibroin (SF) solution and mixed at 60°C to obtain a concentrated spinning solution; the spinning solution was loaded into a 10 mL syringe, the electrospinning pressure was set to 15-20 kV, the flow rate was 0.005-0.02 mL / h, and electrospinning was performed within a receiving distance of 12-20 cm to obtain a 0.2 mm thick fiber membrane. The above fiber membrane was vacuum dried overnight for use.
[0021] (2) Preparation of hydrogel mask: Place the fiber membrane into the Coptis chinensis extract solution, add 6% by mass PVA and 0.6% by mass TSPBA solution, stir thoroughly, and let stand to obtain a hydrogel mask.
[0022] The cell viability of the hydrogel mask was 92%. Figure 1 shown.
[0023] Cell viability test The hydrogel mask prepared in Example 1-3 was made into a small disc with a diameter of 0.3 mm and spread on the bottom of a 96-well plate. Subsequently, a DMEM medium (100 µL, 10% calf serum) containing 2000 fibroblasts (L929 cells) was inoculated into a single well and cultured in a cell culture incubator (37°C, 5% carbon dioxide (CO2) concentration, 95% relative saturated humidity) for 3 days. The culture medium was discarded and the residual culture medium was thoroughly washed with PBS. 100 µL of serum-free DMEM culture medium containing 10% CCK-8 was added to a single well, and after culturing in an incubator (37°C, 5% carbon dioxide (CO2) concentration, 95% relative saturated humidity) for 2 hrs, the absorbance of each well at 450 nm was measured by an enzyme reader under room temperature. Figure 1 The hydrogel mask prepared in Example 1-3 promotes the proliferation activity of fibroblasts. Figure 1 It can be seen that compared with the control group, the hydrogel masks prepared in Examples 1-3 of the present application have cell viability far greater than 80%, and have good biocompatibility.
Claims
1. A method for preparing an inflammation-promoting repairing facial mask, characterized in that: First, a fiber basement membrane is prepared by loading simulated enzymes by electrospinning, and then the basement membrane is immersed in a solution containing a Chinese herbal medicine extract, and polyvinyl alcohol (PVA), the Chinese herbal medicine extract and the basement are integrated with an active oxygen crosslinking agent N1-(4-bromobenzyl)-N3-(4-bromophenyl)-N1,N1,N3,N3-tetramethylpropane-1,3-diamine (TSPBA) to form a hydrogel repair mask, including the following steps: (1) Preparation of fiber spinning membrane: Dissolve the simulated enzyme in a 20% to 60% by mass silk fibroin (SF) solution and mix them evenly at 60°C to obtain a concentrated spinning solution. Load the spinning solution into a 10 mL syringe, set the electrospinning pressure to 15 to 20 kV, and perform electrospinning at a flow rate of 0.005 to 0.02 mL / h within a receiving distance of 12 to 20 cm to obtain a fiber membrane with a thickness of 0.05 to 0.2 mm. The obtained fiber membrane is vacuum dried overnight for later use. (2) Preparation of hydrogel mask: Place the fiber membrane in a solution containing Chinese herbal extract, add 2% to 10% PVA and 0.1% to 10% TSPBA solution, stir thoroughly, and let stand to obtain a hydrogel mask.
2. A method for preparing an inflammation-promoting repairing facial mask according to claim 1, characterized in that The simulated enzyme has the ability to scavenge free radicals and is any one of an iron-based nanozyme, nanogold, a cerium-based metal organic framework material, and a manganese-based metal organic framework material.
3. A method for preparing an inflammation-promoting repairing facial mask according to claim 1, characterized in that The size of the gold nanoparticles is 2-10 nm.
4. A method for preparing an inflammation-promoting repairing facial mask according to claim 1, characterized in that The added amount of the simulated enzyme is 0.1~0.8g.
5. A method for preparing an inflammation-promoting repairing facial mask according to claim 1, characterized in that The Chinese medicine extract is a combination of any one or more of the following components with antibacterial and anti-inflammatory effects, including: coptis root, lithospermum officinale, angelica dahurica, phellodendron amurense, isatis root, and honeysuckle.
6. The method for preparing a pro-inflammatory repair facial mask according to any one of claims 1 to 5, characterized in that: Prepare as follows: (1) Preparation of fiber spinning membrane: 0.2 g of 5 nm enzyme-mimicking gold nanoparticles was dissolved in 30% by mass silk fibroin (SF) solution and mixed at 60 °C to obtain a concentrated spinning solution. The spinning solution was loaded into a 10 mL syringe, and the electrospinning pressure was set to 15-20 kV. The flow rate was 0.005-0.02 mL / h, and electrospinning was performed within a receiving distance of 12-20 cm to obtain a 0.2 mm thick fiber membrane. The obtained fiber membrane was vacuum dried overnight and set aside. (2) Preparation of hydrogel mask: Place the fiber membrane in a solution containing Coptis chinensis extract, add 3% by mass PVA and 0.5% by mass TSPBA solution, stir thoroughly, and let stand to obtain a hydrogel mask.
7. A method for preparing a pro-inflammatory repair facial mask according to any one of claims 1 to 5, characterized in that: Preparation was carried out according to the following steps: (1) Preparation of fiber spinning membrane: 0.2 g of iron-based metal organic framework material was dissolved in 40% silk fibroin (SF) solution, and mixed evenly at 60°C to obtain a concentrated spinning solution; the spinning solution was loaded into a 10 mL syringe, and the electrospinning pressure was set to 15-20 kV, and the flow rate was 0.005-0.02 mL / h. Electrospinning was performed within a receiving distance of 12-20 cm to obtain a 0.2 mm thick fiber membrane. The above fiber membrane was vacuum dried overnight for use; (2) Preparation of hydrogel mask: Place the fiber membrane into the Coptis chinensis extract solution, add 5% by mass PVA and 0.6% by mass TSPBA solution, stir thoroughly, and let stand to obtain a hydrogel mask.
8. The method for preparing a pro-inflammatory repair facial mask according to any one of claims 1 to 5, characterized in that: Preparation was carried out according to the following steps: (1) Preparation of fiber spinning membrane: 0.3 g of Mn-based metal organic framework material was dissolved in 40% silk fibroin (SF) solution and mixed evenly at 60°C to obtain concentrated spinning solution; the spinning solution was loaded into a 10 mL syringe, the electrospinning pressure was set to 15-20 kV, and the flow rate was 0.005-0.02 mL / h. Electrospinning was performed within a receiving distance of 12-20 cm to obtain a 0.2 mm thick fiber membrane. The above fiber membrane was vacuum dried overnight for use. (2) Preparation of hydrogel mask: Place the fiber membrane into the Coptis chinensis extract solution, add 6% by mass PVA and 0.6% by mass TSPBA solution, stir thoroughly, and let stand to obtain a hydrogel mask.
9. A pro-inflammatory repair mask, characterized in that A hydrogel mask based on electrospinning and microenvironment ROS response is prepared according to any one of the methods described in claims 1-8.