Application of transcription factor OsMPS in increasing content of rice 2-acetyl-1-pyrroline

By overexpressing the transcription factor OsMPS in rice, inhibiting the expression of OsBADH2 gene, the problem of difficulty in effectively increasing the 2-AP content in the prior art was solved, and the effect of significantly improving the 2-AP content was achieved, and the fragrance quality of the rice was improved.

CN119684423AActive Publication Date: 2025-03-25HAINAN UNIVERSITY SANYA NANFAN RESEARCH INSTITUTE
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Patent Information

Application Number
CN202510200846.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-24
Publication Date
2025-03-25
Estimated Expiration
2045-02-24

AI Technical Summary

Technical Problem

Research on the synthesis regulation of rice 2-acetyl-1-pyrroline (2-AP) in the prior art mainly focuses on OsBADH2, while transcriptional regulation mechanisms have rarely been reported, making it difficult to effectively increase the 2-AP content of rice.

Method used

By overexpressing the transcription factor OsMPS, the expression of the OsBADH2 gene in rice is inhibited, thereby promoting the synthesis of 2-acetyl-1-pyrroline.

Benefits of technology

It significantly improves the 2-acetyl-1-pyrroline content in rice, improves the fragrance quality of rice, and provides a new way for rice breeding.

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Abstract

The invention relates to the technical field of plant genetic engineering, in particular to application of a transcription factor OsMPS in increasing the content of rice 2-acetyl-1-pyrroline. The amino acid sequence of the transcription factor OsMPS is as shown in SEQ ID NO. 27. According to the invention, through overexpression of the OsMPS gene driven by the constitutive promoter in the rice, the content of 2-acetyl-1-pyrroline in the rice is obviously increased; and the 2-acetyl-1-pyrroline is not detected in the mutant of the knockout line. The method has the advantages that the OsMPS is used as a positive regulation factor to promote the synthesis of the rice 2-acetyl-1-pyrroline, and plays an important role in improving the rice fragrance quality.
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