Microflora for in-situ enrichment of caenorhabditis elegans in red soil and application of flora

Abstract

The invention discloses a flora for in-situ enrichment of nematode in red soil and application of the flora. The flora comprises one or more of bacteria A, bacteria B, bacteria C and bacteria D, the bacteria A are Cupriavidus necator and a bacterial strain YTR-C1, the bacteria B are Sinorhizobium sp. And a bacterial strain YTR-S1, the bacteria C are Mesorhizobium amorphae and a bacterial strain YTR-M3, the bacteria D are Ralstoniapickettii and a bacterial strain YTR-R242, the strains are used in a combined mode, the concentration ratio of the strains is (1-10): (1-10): (1-10): (1-10): (1-10), and the number of the caenorhabditis in red soil can be increased, so that growth of crops is promoted.

Description

Technical Field

[0001] The invention relates to the technical field related to soil animal breeding, and in particular to a bacterial community used for in-situ enrichment of Cephalophyllum nematodes in red soil and its application. Background Art

[0002] Phosphorus is one of the essential nutrients for plant growth. It is not only a component of many important organic compounds in plants, but also participates in various physiological processes in plants in many ways. 74% of my country's cultivated land soils are deficient in phosphorus, and more than 95% of the phosphorus in the soil is ineffective phosphorus, which is difficult to be absorbed and utilized by plants. In agricultural production, highly water-soluble phosphorus fertilizers are often used to meet the plant's demand for phosphorus. However, after phosphorus fertilizers are applied to the soil, they tend to form insoluble phosphates, which are quickly adsorbed and fixed by soil minerals or microorganisms. The southeastern hilly area is the main production area of ​​tropical and subtropical economic forestry, economic crops and grain (rice) in my country, and plays an important role in regional sustainable development. Red soil, as the main medium- and low-yield soil in the region, contains a large amount of free iron and aluminum oxides, which makes phosphorus more easily fixed and shows serious phosphorus deficiency. The utilization rate of phosphorus in the season is only 10%-25%. The soil phosphorus deficiency and reduced productivity caused by red soil acidification have seriously threatened the stability of the ecosystem and the efficient use of resources.

[0003] Nematodes are one of the main functional groups of soil animals and the most abundant metazoan in the soil. Soil nematodes are huge in number and rich in nutritional groups. They are widely distributed in soils of various habitats. The proportion of bacteria-eating nematodes in the soil rhizosphere is as high as about 90%. Nematodes have the characteristics of short generation cycle and sensitive response to environmental changes. They are often used as indicator organisms for changes in farmland management measures. The ecological index of nematodes can provide unique information about soil ecosystems. Nematodes occupy a key central position in the soil food web. Through selective predation, they not only change the biomass, diversity and community composition of microorganisms, but also directly affect the survival adaptability and health of nematodes. Nematode-microorganism interactions regulate the energy transfer and carbon, nitrogen and phosphorus nutrient conversion and storage processes of soil ecosystems, which plays an important role in maintaining the stability of the soil food network structure and functional diversity. Soil nematodes are dominated by free-living bacteria-eating nematodes. Bacteria-eating nematodes promote plant growth by preying on soil microorganisms and releasing nutrients held in microorganisms. In order to alleviate phosphorus stress in red soil and improve the biological effectiveness of phosphorus, the indigenous bacteria-feeding nematodes of the genus Cephalophyllum can be enriched in situ from dryland red soil, and their culture conditions can be optimized. The nematodes of the genus Cephalophyllum can be applied to low-yield dryland red soil in order to increase the phosphatase activity and the content of available phosphorus storage capacity in red soil, thereby increasing crop yields and improving quality. Summary of the invention

[0004] In view of the technical problems and practical needs of developing a fast and efficient in situ propagation method for nematodes in the prior art, the present invention provides a bacterial community and application for in situ enrichment of Cephalophyllum nematodes in red soil.

[0005] To achieve the above object, the present invention adopts the following technical scheme: a bacterial community for in situ enrichment of Cephalotaxus nematodes in soil, characterized in that it comprises one or more of bacteria A, bacteria B, bacteria C, and bacteria D, wherein the concentration ratio of bacteria A, bacteria B, bacteria C, and bacteria D is (1-10):(1-10):(1-10):(1-10); wherein,

[0006] The bacterium A is Cupriavidus necator, strain YTR-C1, deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration on May 16, 2023, with the deposit number CGMCC27358;

[0007] The bacterium B is Sinorhizobium sp., strain YTR-S1, which was deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration on September 18, 2020, with the deposit number: CGMCC20681;

[0008] The bacterium C is Mesorhizobium amorphae, strain YTR-M3, which was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on April 9, 2019, with the deposit number: CGMCC17532; the bacterium D is Ralstonia pickettii, strain YTR-R242, which was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on October 16, 2024, with the deposit number CGMCC32269.

[0009] Preferably, in the strain combination, the abundance of one bacteria is higher than the sum of the other three bacteria.

[0010] Preferably, the effective viable count of bacteria A, bacteria B, bacteria C, and bacteria D is 104-108 cells / gram.

[0011] Preferably, the strain group is inoculated into red soil and decomposed pig manure substrates.

[0012] The invention provides an application of a bacterial community for in-situ enrichment of Cephalotaxus nematodes in soil, which is used for planting corn and rapeseed.

[0013] Preferably, the mass ratio of decomposed pig manure to red soil is (3-7):1.

[0014] The invention provides an adaptive culture matrix for nematode enrichment, the components of which are red soil to be inoculated with nematodes, decomposed pig manure and the bacterial community.

[0015] Beneficial effects:

[0016] The present invention is simple to operate, improves the in-situ growth environment of nematodes, and improves the reproductive capacity of the genus Cephalotaxus. Adding the nematode-rich culture matrix to the red soil in farmland can promote the activation of red soil phosphorus, increase soil phosphatase activity and phosphorus effectiveness, and improve corn yield and quality. Compared with the prior art, the present invention has the following advantages:

[0017] (1) In situ enrichment of dominant nematodes indigenous to farmland red soil, by adding microbial agents and agricultural organic waste, can enhance the survival rate and reproduction capacity of nematodes in red soil, and increase the total number of bacteria-feeding nematodes and the number of Cephalophyllum nematodes. Cephalophyllum nematodes cultivated by the technical solution of the present invention grow rapidly and have enhanced reproduction capacity. At the same time, the in situ enrichment method of nematodes has low production cost, simple technology, and is easy to promote on a large scale.

[0018] (2) The application of the genus Cephalotaxus to red soil increases the activity of alkaline and acid phosphatase in the red soil, promotes the activation and release of phosphorus in the red soil, and increases the effective phosphorus content in the red soil.

[0019] (3) The application of the Cephalotaxus nematode to red soil increases the absorption and utilization of nutrients in the red soil by corn, improves the growth of corn, significantly increases corn yield and aboveground and root biomass, and increases leaf soluble sugar, leaf soluble protein, and leaf indoleacetic acid content. The Cephalotaxus nematode cultured in situ enrichment of the present invention has obvious effects of increasing yield and improving quality after application, and is suitable for large-scale use in field crop production areas. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The changes in the number of nematodes in the adaptive culture matrix with different ratios;

[0021] Figure 2 It is a nematode of the genus Cephalophyllum in red soil;

[0022] Figure 3 The reproduction of nematodes under feeding conditions with single bacterial colonies isolated and enriched in different ways;

[0023] Figure 4 Changes in phosphatase activity and available phosphorus content in red soil around maize under high, medium and low nematode exposure conditions. DETAILED DESCRIPTION

[0024] The present invention is further explained below in conjunction with the embodiments. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and should not and cannot be used to limit the protection scope of the present invention.

[0025] Example 1

[0026] The nematodes were isolated using a modified shallow plate-sucrose centrifugal flotation continuous extraction method and counted using a Motic SMZ-168 stereo microscope. The counted nematode aqueous solution was completely poured into a 50 mL centrifuge tube, and the upper layer of liquid was aspirated after standing for about 12 hours. 9 mL of the nematode aqueous solution was retained and placed in a water bath at 60°C for 5 minutes. After cooling, 1 mL of formalin solution was added to each sample to complete the preservation; 150-200 well-preserved nematodes were aspirated from a small plastic dish and placed on a glass slide, covered with a coverslip and sealed, and the nematodes were identified to the genus under an optical microscope (Olympus BX50).

[0027] The nematodes cultivated in the present invention are of the genus Cephalophyllum, order Rhabdiformes, suborder Mesanophyllum, family Cephalophyllum. Figure 1 As shown in the figure, the biological characteristics of the genus Cephalophyllum are as follows: the worm is of medium size, and after heat killing, it bends slightly toward the abdomen. The cuticle has fine rings, with 2 to 4 lateral bands extending to the end of the tail. The lip region is slightly constricted, without head protrusions, and the lip protrusions are low and round. The anterior and posterior parts of the esophagus are cylindrical, the isthmus is narrow, and the posterior esophageal bulb is oval with obvious butterfly-shaped ossified flaps. The nerve ring surrounds the posterior part or isthmus of the esophagus. There are cervical papillae. The lateral tail gland is located near the middle of the tail. The female has a single gonad extending forward, the ovary is folded twice, with a spermatheca and a posterior uterine sac, the vulva is flat or protruding, the tail is conical, and the tail end is blunt or pointed. The girdle of the male is wedge-shaped, the tail is conical, arched and bent toward the abdomen, and the end has a short hook-like tail tip.

[0028] Example 2

[0029] Four bacterial species were isolated and enriched in the in situ red soil. Bacteria A was Cupriavidus necator, strain YTR-C1, which was deposited in the General Microbiology Center of China National Microorganism Culture Collection on May 16, 2023, with the accession number CGMCC27358. Bacteria B was Sinorhizobium sp., strain YTR-S1, which was deposited in the General Microbiology Center of China National Microorganism Culture Collection on September 18, 2020, with the accession number CGMCC20681. Bacteria C was Mesorhizobium amorphae, strain YTR-M3, which was deposited in the General Microbiology Center of China National Microorganism Culture Collection on April 9, 2019, with the accession number CGMCC17532. Bacteria D is Ralstonia pickettii, strain YTR-R242, deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration on October 16, 2024, with a deposit number of CGMCC32269. The above bacteria have been disclosed in patent documents, as follows: Sinorhizobium sp., patent number: ZL 202011189260.X; Mesorhizobium amorphae, patent number: ZL 201911119326.5; Cupriavidus necator, patent number: ZL 202310872862.2; Ralstonia pickettii, application number: 202411659347.7. The effect of bacteria on nematode growth was analyzed by feeding experiments, and the steps are as follows:

[0030] 1. Red soil was collected from a long-term field experiment, which was built in the Yingtan Farmland Ecosystem National Field Research Station in Jiangxi Province (116°55′E, 28°13′N). The experimental area has a mid-subtropical monsoon climate, with an average annual temperature of 17.8℃, an average annual precipitation of 1795mm, and a frost-free period of 262d.

[0031] 2. Collect red soil from the field using the knife-ring method, mix well, and divide it into three portions. Take 5g of red soil from each portion to extract DNA from the red soil and perform high-throughput sequencing. Add the separated and enriched bacteria A, bacteria B, bacteria C, and bacteria D to 50g of sterilized red soil in sequence, with a concentration of 10 6 Each treatment was added with 45g of in situ red soil and 5g of sterilized and decomposed pig manure. The amount of nematodes (adults) was 300 / 100g of soil. The indoor culture conditions were 60%-70% of the soil moisture to the field water holding capacity, cultured in the dark for 12 days, and the culture temperature was 12-28℃.

[0032] 3. After 12 days of culture, the nematodes were isolated using a modified shallow plate-sucrose centrifugal flotation continuous extraction method and counted using a Motic SMZ-168 stereo microscope. Figure 2 As shown, bacteria A, bacteria B, bacteria C and bacteria D all increased the number of nematodes. The total number of nematodes increased by 6.2, 1.5, 6.3 and 6.5 times, respectively. The number of bacteria-feeding nematodes increased by 65.3, 20.2, 66.5 and 68.4 times, respectively. The number of genera increased by 195.3, 59.5, 195.8 and 204.7 times, respectively.

[0033] 4. Extract DNA from red soil according to the operating steps provided by the Mo-Bio Power Soil DNA Extraction Kit (Mo-Bio Laboratories, Inc., CA, USA), and use Nano Drop Spectrophotometry (Nano Drop Technologies, Wilmington, DE, USA) to detect the quality and quantity of DNA. The extracted DNA was stored in dry ice and transported to Lingen Company for high-throughput sequencing. The sequencing primers were 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 907R (5'-CCGTCAATTCCTTTGAGTTT-3'), which were used to amplify the V4-V5 region of the bacterial 16S rRNA gene. The species and abundance information of bacteria in red soil were obtained, and it was known that the abundance ratio of bacteria A, bacteria B, bacteria C and bacteria D was approximately 6:1:5:4.

[0034] Example 3

[0035] The steps of obtaining a mixture of red soil, agricultural organic waste and microbial agent under the most suitable ratio as an adaptive culture matrix for nematodes in a natural field environment and obtaining nematodes preserved in the adaptive culture matrix are as follows:

[0036] 1. The components of the adaptive culture matrix are red soil to be inoculated with nematodes, decomposed pig manure and bacterial agents.

[0037] 1) Collect red soil from the test plot and pass it through a 2mm sieve for nematode inoculation. Pig manure is produced from a local pig farm. Collect fresh pig manure and add water to 80% of the water content. Mix evenly, add 10% of red soil to the pig manure, store it in a fermentation box (70cm long, 60cm wide, 50cm high), and aerate it. The initial heating temperature is 40℃, the mid-term heating temperature is 70℃, and the late heating temperature is 90℃. The entire cycle time is 30d. In the early stage, the compost is turned and mixed every 2d. In the later stage, the frequency of turning the compost can be accelerated to accelerate water evaporation and composting process. The application rate of mature pig manure is 5 tons / hm 2(Dry basis). After the pig manure is decomposed, mix the decomposed pig manure with the red soil collected in situ in the test plot at a mass ratio of 3:1, 5:1, and 7:1, and fill it into a plastic container with holes at the bottom. When filling, fill 5 cm thick culture matrix each time, use a spray pot to evenly spray the atomized water into the soil, and adjust the soil moisture to 60%-70% of the field water holding capacity.

[0038] 2) Sterile water containing nematodes was inoculated by spraying, with an inoculation amount of 300 nematodes / 100 grams of soil. The inoculated microbial agents were bacteria A, bacteria B, bacteria C and bacteria D, with a concentration ratio of T1: 6:1:5:4, T2: 4:4:4:4, T3: 10:2:2:2, T4: 2:10:2:2, T5: 2:2:10:2, T6: 2:2:2:10, and the effective viable count was 10 4 Pieces / gram soil, 10 6 Pieces / gram soil, 10 8 / g soil, providing a direct food source for bacteria-eating nematodes. At the same time, a control group T0 was set up without adding bacterial agents. The inoculated bacterial concentration was mainly designed based on the original concentration ratio of different bacteria in red soil. For the same bacterial concentration ratio, one absolute dominant bacteria (the abundance of one bacteria is higher than the sum of the other three bacteria, to see the dominant effect of this bacteria) was used for design; the effective live bacteria count was mainly based on the bacterial concentration in the soil, which was about 10 7 / g soil, set low concentration (10 4 / g soil), slightly lower than the natural abundance (10 6 / g soil) and slightly higher than the natural abundance (10 8 During the in situ enrichment culture of nematodes, the moisture of the culture medium is kept stable, and the adaptive culture medium is cultured in a natural environment in the dark for 10-12 days at a culture temperature of 12-28°C.

[0039] 2. After the nematodes are cultured in the adaptive matrix, the nematodes are separated using an improved shallow plate-sucrose centrifugal flotation continuous extraction method and counted using a Motic SMZ-168 stereo microscope. Pour the counted nematode aqueous solution completely into a 50 mL centrifuge tube, let it stand for about 12 hours, then aspirate the upper liquid, retain 9 mL of the nematode aqueous solution, and bathe it in a water bath at 60°C for 5 minutes. After cooling, add 1 mL of formalin solution to each sample to complete the preservation; 150-200 well-preserved nematodes are drawn from a small plastic dish and placed on a glass slide, which is then covered with a coverslip and sealed. The nematodes are identified to their genus under an optical microscope (Olympus BX50). The results showed that after the nematodes were enriched in situ in the adaptive matrix, the total number of nematodes and bacteria-feeding nematodes increased significantly, especially the number of nematodes of the genus Cephalotaxus increased extremely significantly. See the results of the nematode number for details. Figure 1:Compared with the control group, all treatment groups with added bacteria can increase the number of nematodes. The mass ratio of decomposed pig manure to red soil collected in situ in the experimental plot was 5:1, and the concentration of bacterial agent added was 10 6 The total number of nematodes, the number of bacteria-eating nematodes and the number of genus nematodes were the highest when the concentration ratio of bacteria A, bacteria B, bacteria C and bacteria D was T4. Under this condition, after in situ enrichment culture, the total number of nematodes can increase by 9.2 times (0.03-4.38 times higher than other treatments), the number of bacteria-eating nematodes increases by 115.6 times (0.12-5.19 times higher than other treatments), and the number of genus Cephalophyllum increases by 192.3 times (0.12-5.49 times higher than other treatments).

[0040] Example 4

[0041] Corn and rapeseed field planting trials

[0042] 1. Experimental treatment: This experiment set up two treatments, applying high, medium and low doses (100 / 100 g dry soil, 500 / g soil and 1000 / 100 g soil) of Cephalophyllum nematodes and a control treatment (CK) without nematodes. The experimental plot area was 20m 2 (5m×4m), with 3 replicates for each treatment.

[0043] 2. Varieties and planting methods: The corn seeds are Suyu No. 24, which has excellent characteristics such as high disease resistance, drought resistance and high temperature resistance. The sowing rate is generally 20kg / hm 2 , the row spacing is 20cm×40cm, the sowing depth is 10-15cm, and the planting density is 68,000 plants / hm 2 The rapeseed is Qingyou No. 8. The sowing rate is 4kg / hm 2 The row spacing is 15cm×30cm, the sowing depth is 3-4cm, and the planting density is about 90,000 plants / hm2. 2 .

[0044] 3. Nutrient management: In corn planting, the application rates of urea, calcium magnesium phosphate fertilizer and potassium fertilizer are 152.72 kg N / hm 2 、116.20kg P2O5 / hm 2 and 166.32kg K2O / hm 2 The pig manure is fully decomposed through high temperature composting technology. After decomposition, the moisture content of the pig manure is less than 50%. The application rate of pig manure is 5 tons / hm 2 (Dry basis) to replace 60% of chemical fertilizers. Phosphorus and potassium are all used as base fertilizers, 70% of nitrogen fertilizers are used as base fertilizers, and 30% are used as topdressing during the small trumpet period. Rapeseed only uses the same amount of chemical fertilizers.

[0045] 4. Application of nematodes: According to the inoculation density of 100 bacteria-feeding nematodes / 100 g dry soil, 500 / g soil and 1000 / 100 g soil, the adaptive matrix together with the nematodes was applied to the test plot and manually plowed into the 0-30 cm soil layer of the red soil.

[0046] 5. Field management: During the planting period of corn and rapeseed, adjust the soil moisture to 60%-70% of the field water holding capacity. During the critical growth periods of crops such as the seedling stage and flowering stage, timely irrigate according to the water demand of crops to ensure normal growth of crops. Irrigate in time when drought occurs during the corn growth period, and drain in time when heavy rainfall occurs. Except for not spraying pesticides, field management such as manual weeding and topdressing shall be carried out in accordance with local practices.

[0047] 6. The field test was carried out until the corn and rapeseed matured. The rhizosphere soil of the plants was collected and the alkaline phosphatase activity and available phosphorus content of each treatment red soil were measured. The results are as follows: Figure 3 As shown: Compared with the high and low release conditions, the treatment with medium release of Cephalotaxus nematodes had the highest acid and alkaline phosphatase activities, available phosphorus content, and corn yield, which were increased by 64.7%, 30.6%, 39.8% and 10.2%-50.5% respectively compared with the control CK.

[0048] 7. The field test was carried out until the corn and rapeseed matured, and the corn plants and rapeseed grains were harvested. The aboveground and root biomass of corn, soluble sugar in leaves, soluble protein and indoleacetic acid in leaves were measured. The results showed that compared with the high and low release conditions, the height of corn plants, the dry weight of straw, the soluble sugar, soluble protein and indoleacetic acid content in plant leaves of the corn plants with the addition of the medium release of the genus Cephalotaxus nematode were the highest. Under this condition, the dry weight of corn straw was 29.5% higher than that of CK, and the dry weight of the root system was 18.7% higher; the soluble sugar, soluble protein and indoleacetic acid content in the leaves of the plants treated with the addition of the genus Cephalotaxus nematode were 33.8%, 24.5% and 29.9% higher than those of CK, respectively. The rapeseed grain yield increased by 50.5% compared with the control CK.

[0049] The present invention feeds four kinds of bacteria to nematodes in laboratory conditions in turn, and analyzes the effects of the four kinds of bacteria on the growth of nematodes. The nematodes used here are bacteria-eating nematodes; (2) weighing red soil from the test plot, adding agricultural organic waste and bacterial agents of different concentrations, and selecting the red soil-agricultural organic waste-agent mixture under the optimal ratio as the adaptive culture matrix of nematodes in the field natural environment through optimization experiments, and obtaining nematodes preserved in the adaptive culture matrix; (3) backfilling the adaptive culture matrix rich in bacteria-eating nematodes into the soil of the test plot according to high, medium and low feeding amounts. The soil of the test plot is managed in the field to maintain soil moisture stability, and the growth and yield changes of corn and rapeseed in the field under different nematode feeding conditions are detected. The present invention improves the in-situ growth environment of nematodes, and increases the reproductive capacity and yield of the genus Cephalophyllum. Based on the long-term positioning experiment of biological introduction at the Yingtan Red Soil Ecological Experimental Station of the Chinese Academy of Sciences, the total number of soil nematodes and the number of genus Cephalophyllum in red soil are increased. Field experiments showed that the addition of Cephalotaxus nematodes significantly increased the activity of alkaline and acid phosphatase and the content of available phosphorus in red soil, and improved the growth and quality of corn and rapeseed.

[0050] Finally, the above are only some specific embodiments of the present invention, and do not limit the scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made to the technical solution of the present invention without departing from the principle of the present invention, which should be regarded as the protection scope determined by the present invention.

Claims

1. A bacterial community for in situ enrichment of Cephalotaxus nematodes in soil, characterized in that: Including bacteria A, bacteria B, bacteria C, bacteria D, the concentration ratio of bacteria A, bacteria B, bacteria C, bacteria D is (1-10): (1-10): (1-10): (1-10); wherein, The bacterium A is Cupriavidus necator, strain YTR-C1, deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration on May 16, 2023, with the deposit number CGMCC27358; The bacterium B is Sinorhizobium sp., strain YTR-S1, which was deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration on September 18, 2020, with the deposit number: CGMCC20681; The bacterium C is Mesorhizobium amorphae, strain YTR-M3, which was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on April 9, 2019, with the deposit number: CGMCC17532; the bacterium D is Ralstonia pickettii, strain YTR-R242, which was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on October 16, 2024, with the deposit number CGMCC32269.

2. The bacterial community for in situ enrichment of Cephalotaxus nematodes in soil according to claim 1, characterized in that: In the strain combination, the abundance of one bacterium is higher than the sum of the other three bacteria.

3. The bacterial community for in situ enrichment of Cephalotaxus nematodes in soil according to claim 1, characterized in that: The effective viable count of bacteria A, bacteria B, bacteria C, and bacteria D is 10 4 -10 8 Pieces / gram of soil.

4. The bacterial community for in situ enrichment of Cephalotaxus nematodes in soil according to claim 3, characterized in that: The strain group is inoculated into red soil and decomposed pig manure substrates.

5. An adaptive culture matrix for in situ enrichment of nematodes, characterized in that: The components include red soil to be inoculated with nematodes, decomposed pig manure and the bacterial flora according to any one of claims 1 to 5.

6. The use of the bacterial community for in situ enrichment of Cephalotaxus nematodes in soil according to claim 1, characterized in that: Used for corn and rapeseed cultivation.

7. The use of the bacterial community for in situ enrichment of Cephalotaxus nematodes in soil according to claim 6, characterized in that: The mass ratio of decomposed pig manure to red soil is (3-7):1.

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