Ammonia nitrogen degrading bacterium and application thereof

Abstract

The invention discloses an ammonia nitrogen degrading bacterium and application thereof, and belongs to the technical field of biology. The bacterial strain is Pseudomonas ruukawai and is named as JLSH1, the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No.1.62473, and the sequence of a 16s rDNA (Ribosomal Deoxyribose Nucleic Acid) fragment of the bacterial strain is as shown in SEQ ID NO.1. The invention also discloses a method for preparing the pseudomonas ruukawai strain. According to the characteristics of ammonia nitrogen, strains capable of degrading ammonia nitrogen are screened from sewage of a refining sewage treatment plant through a microbial technical means. Experiments prove that the degrading bacterium disclosed by the invention has relatively high ammonia nitrogen degradation activity, and the ammonia nitrogen degradation rate can reach 99.21%. The ammonia nitrogen degrading bacterium JLSH1 provided by the invention can be applied to degradation of ammonia nitrogen in a polluted environment, and has a good application value.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and specifically relates to an ammonia nitrogen degrading bacterium and application thereof. Background Art

[0002] Ammonia nitrogen, as a kind of water pollutant, poses a huge threat to human health. Therefore, the treatment of wastewater containing ammonia nitrogen has attracted people's attention. Ammonia nitrogen in sewage appears as free ammonia (NH3) and ionic ammonia salts (NH4 + ), the two can be transformed into each other. When the pH value of the solution is high, NH3 will be produced, releasing a strong pungent odor.

[0003] In the actual operation of the sewage treatment plant, the sewage treatment biochemical reaction tank will be affected by the unstable types and contents of pollutants in the upstream water, resulting in severe impact on the activated sludge, instability of the biochemical reaction system, unstable ammonia nitrogen degradation effect, and excessively high export ammonia nitrogen index, which will affect the sewage treatment effect.

[0004] Currently, glucose and disodium hydrogen phosphate are often supplemented to change the carbon-nitrogen-phosphorus ratio or to supplement sludge from other sewage treatment plants, but the effect is not good. Among the above methods, the reason why the former is not effective may be that it is preferentially used by non-ammonia nitrogen-degrading bacteria or metazoan microorganisms, making non-functional microorganisms the dominant flora. The reason why the latter is not effective may be that the ammonia nitrogen-degrading microorganisms in other types of sewage will not adapt to the local environment and are likely to antagonize the microorganisms in the original activated sludge, resulting in a decrease in effect.

[0005] Therefore, it is of great significance to screen the dominant bacteria that degrade ammonia nitrogen in the wastewater of the biochemical aeration tank of the refinery by adopting the enrichment and separation method, identify the strains, and then apply them to the degradation of ammonia nitrogen in the refinery wastewater. Summary of the invention

[0006] Purpose of the invention: The purpose of the present invention is to provide an ammonia nitrogen-degrading bacteria and its application in view of the shortcomings of the prior art. The present invention uses petroleum refining wastewater generated by the petroleum and petrochemical industry as raw material, screens and separates a strain that can efficiently degrade environmental ammonia nitrogen, and provides a method for degrading ammonia nitrogen using the strain.

[0007] The strain JLSH1 screened out by the present invention belongs to Pseudomonas furukawaii. The strain JLSH1 of the present invention can independently complete the function of degrading ammonia nitrogen without relying on the nitrification of the previous microorganisms, and the degradation rate in the refinery wastewater can be as high as 99.21%.

[0008] Technical solution: The purpose of the present invention is achieved through the following technical solution:

[0009] The invention provides an ammonia nitrogen degradation strain, which is Pseudomonas furukawaii, named as JLSH1, with a preservation number of CGMCC No.1.62473, and a sequence of a 16s rDNA fragment thereof is shown as SEQID NO.1.

[0010] SEQ ID NO.1:

[0011]

[0012] The bacterial strain JLSH1 of the present invention has yellow colony edges, an elliptical shape, a smooth and opaque surface, and a certain viscosity, and the bacteria are rod-shaped in a microscope photograph.

[0013] The strain was deposited in the General Biological Center of China Microorganism Culture Collection Administration (CGMCC for short, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Building 1, Beichen West Road, Chaoyang District, Beijing, China) on November 14, 2024, with the deposit number: CGMCC No1.62473, and the classification name is Pseudomonas furukawaii.

[0014] The strain is screened and separated from petroleum refining wastewater; the petroleum refining wastewater is extracted from the refining wastewater biological aeration tank of the petroleum refining water purification workshop.

[0015] The present invention also provides a method for isolating the ammonia nitrogen-degrading strain, which is characterized by comprising the following steps:

[0016] (1) Weighing the sewage, adding it to a liquid culture medium for screening ammonia nitrogen-degrading strains for enrichment, and shaking at a constant temperature of 35° C. and 180 rpm for 4-7 days to obtain a culture solution;

[0017] (2) taking the culture solution obtained in step (1), adding it to another liquid culture medium for screening ammonia nitrogen-degrading strains for subculture, and subculturing for 3-6 times to obtain a culture solution;

[0018] (3) Take the culture solution obtained in step (2) and prepare 10 -3 , 10 -4 , 10 -5 The diluent is taken out, and the diluent is spread on a solid culture medium for screening ammonia nitrogen degradation strains to separate and preliminarily screen out characteristic colonies;

[0019] (4) Pick the characteristic colonies obtained in step (3) for plate streaking isolation and purification, and inoculate the purified strains into LB liquid culture medium and culture at 35° C. and 180 rpm for 24 h;

[0020] (5) Analyze the growth performance of the different bacterial strains isolated and their efficiency in degrading ammonia nitrogen in the culture medium, and finally obtain the ammonia nitrogen degrading strain.

[0021] The components of the liquid culture medium for screening ammonia nitrogen-degrading strains are: C6H5Na3O7 1g / L; (NH4)2SO4 1g / L; K2HPO4 1.5g / L; KH2PO4 0.5g / L; MgSO4·H2O 0.2g / L; NaCl 6g / L; pH 7.2-7.4;

[0022] The components of the solid culture medium for screening ammonia nitrogen-degrading strains are: C6H5Na3O7 1g / L; (NH4)2SO4 1g / L; K2HPO4 1.5g / L; KH2PO4 0.5g / L; MgSO4·H2O 0.2g / L; NaCl 6g / L; agar powder 15g / L; pH 7.2-7.4.

[0023] The components of the LB liquid culture medium are: 10 g of peptone, 5 g of yeast powder, 10 g of sodium chloride, and the volume is supplemented to 1 L with deionized water.

[0024] The culture medium of the present invention is sterilized at 121° C. for 20 min.

[0025] Specifically, the isolation method of the strain comprises the following steps:

[0026] (1) Weighing sewage from a biochemical aeration tank, adding it to a liquid culture medium for screening ammonia nitrogen-degrading strains for enrichment, and culturing it in a constant temperature shaker at 35°C and 180 r / min for 4-7 days;

[0027] (2) taking the culture solution obtained in step (1) and adding it to another liquid culture medium for screening ammonia nitrogen-degrading strains for subculturing, and subculturing for 3 to 6 times;

[0028] (3) Take the culture solution obtained in step (2) and prepare 10 -3 , 10 -4 , 10 -5 The diluent is used to ensure that the strain does not grow too densely in the solid culture medium to avoid interference during the growth process and affect the observation. The diluent is taken out and spread on the solid culture medium for screening ammonia nitrogen degradation strains to preliminarily screen out characteristic colonies; the characteristic colonies are excellent strains with high ammonia nitrogen degradation activity. The role of dilution is to make the content of degradation bacteria in the fermentation liquid moderate, which is conducive to the next step of preliminary screening.

[0029] (4) Pick a single characteristic colony obtained in step (3) and perform 2-4 streaking separations on an LB plate to ensure that pure bacteria are obtained as much as possible. The purified single colony is inoculated into LB liquid culture medium for culture.

[0030] (5) Analyze the growth performance of different bacterial strains isolated and their efficiency in degrading ammonia nitrogen in the culture medium, and screen the strain with the best ammonia nitrogen degradation effect.

[0031] The bacterial liquid was inoculated into the MSM basic liquid medium containing 100 mg / L ammonia nitrogen at a 1% inoculation amount, and cultured in a constant temperature shaker at 35°C and 180 r / min for 7 days, and repeated 3 times in parallel. The degradation rate of ammonia nitrogen was measured by phenol sodium hypochlorite colorimetry.

[0032] Steps (1), (2), (3) and (4) are preliminary screening of strains, the purpose of which is to screen out strains with different appearances and morphologies and capable of degrading ammonia nitrogen in the culture medium.

[0033] The present invention also provides the use of the ammonia nitrogen-degrading strain in degrading ammonia nitrogen-contaminated water body, wherein the ammonia nitrogen-contaminated water body is refinery wastewater.

[0034] The conditions for the strain to degrade ammonia-nitrogen polluted water bodies are 25-35° C. and pH 6-8.

[0035] The inoculation amount of the strain for degrading ammonia-nitrogen polluted water is 0.5-3%.

[0036] The carbon-nitrogen ratio of the strain in degrading ammonia-nitrogen-contaminated water is 4.5-12.5.

[0037] The method for degrading ammonia-nitrogen-contaminated water by the strain comprises the following steps:

[0038] (1) Activation of strains: strain JLSH1 was inoculated from a slant plate into LB liquid culture medium and cultured at 30-35°C and 180 rpm for 24 h to prepare a bacterial solution;

[0039] (2) Strain degradation process: The above bacterial solution is inoculated into the refinery wastewater, the pH value is 6-8, the inoculation amount is 0.5-3%, and the culture is carried out at 30-35°C, 150r / min constant temperature shaking for 8h, which can effectively degrade the ammonia nitrogen in the refinery wastewater.

[0040] Beneficial effects:

[0041] According to the characteristics of ammonia nitrogen, the present invention separates and screens a strain JLSH1 capable of degrading ammonia nitrogen from a specific environment by means of microbial technology. Experiments show that the ammonia nitrogen degrading bacteria JLSH1 of the present invention has high ammonia nitrogen degradation activity, and the ammonia nitrogen degradation rate can reach 99.21%. The ammonia nitrogen degrading bacteria JLSH1 provided by the present invention can be applied to the degradation of ammonia nitrogen in polluted environments and refining wastewater, and has good application value.

[0042] The strain of the present invention has the following advantages when used as a bacterial agent to degrade ammonia nitrogen in refining wastewater:

[0043] 1. Pure strains are highly targeted and can react faster to the optimization of ammonia nitrogen degradation process;

[0044] 2. Compared with the supplementation of ex situ sludge, the “indigenous strains” are more adaptable to the sewage in the refinery wastewater and are not prone to antagonism;

[0045] 3. After obtaining a single strain, the bacterial agent is made by fermentation and expansion, which can be used for future process adjustments to special working conditions. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 The morphological observation results of strain JLSH1 are shown in Figure 1. Figure 1 A is the colony morphology of strain JLSH1 in LB medium;

[0047] Figure 1 B is the cell morphology of strain JLSH1 (1000x).

[0048] Figure 2 Phylogenetic tree of the 16S rDNA domain sequence of strain JLSH1.

[0049] Figure 3 This is the C / N single factor degradation rate diagram.

[0050] Figure 4 This is the single factor degradation rate diagram of inoculum size.

[0051] Figure 5 This is the pH single factor degradation rate diagram.

[0052] Figure 6 is the temperature single factor degradation rate.

[0053] Figure 7 It is the degradation rate of strain JLSH1 in pure system (inorganic salt medium).

[0054] Figure 8 is the degradation rate of strain JLSH1 in refinery wastewater. DETAILED DESCRIPTION

[0055] The technical solution of the present invention is described in detail below through specific embodiments, but the protection scope of the present invention is not limited to the embodiments.

[0056] The components of the liquid culture medium for screening ammonia nitrogen degrading bacteria used in the present invention are: C6H5Na3O7 1g / L; (NH4)2SO4 1g / L; K2HPO4 1.5g / L; KH2PO4 0.5g / L; MgSO4·H2O 0.2g / L; NaCl 6g / L; pH 7.2-7.4, sterilization at 121°C for 20min.

[0057] The solid culture medium for screening ammonia nitrogen-degrading strains used in the present invention comprises the following components: C6H5Na3O7 1g / L; (NH4)2SO4 1g / L; K2HPO4 1.5g / L; KH2PO4 0.5g / L; MgSO4·H2O 0.2g / L; NaCl 6g / L; agar powder 15g / L; and pH 7.2-7.4.

[0058] The components of the LB liquid medium used in the present invention are: 10g peptone, 5g yeast powder, 10g NaCl, the volume is supplemented to 1L with deionized water, and sterilized at 121°C for 20min. The LB solid medium is supplemented with 15g / L agar powder on the basis of the above.

[0059] The components of the MSM basic liquid culture medium used in the present invention are C6H5Na3O7 1g / L; (NH4)2SO4 1g / L; K2HPO4 1.5g / L; KH2PO4 0.5g / L; MgSO4·H2O 0.2g / L; NaCl 6g / L; pH 7.2-7.4. The MSM solid culture medium is supplemented with 15g / L agar powder on the basis of the above.

[0060] Example 1 Screening and Isolation of Strain JLSH1

[0061] The present invention collects sludge samples from the 12# aeration tank in the water purification area of ​​Nanjing Jinling Petrochemical.

[0062] (1) Weigh 5 mL of sewage from the biochemical aeration tank, add it to 100 mL of ammonia nitrogen-degrading bacteria screening liquid culture medium for enrichment, and culture it in a constant temperature shaker at 35°C and 180 r / min for 7 days;

[0063] (2) The culture solution obtained in step (1) was added to a new liquid culture medium for screening ammonia-nitrogen-degrading bacteria at an inoculum volume of 5% for subculturing, and the subculturing was repeated 5 times;

[0064] (3) Take the culture solution obtained in step (2) and prepare 10 -3 , 10 -4 , 10 -5 The diluent is used to ensure that the strain does not grow too densely in the solid culture medium to avoid interference during the growth process and affect the observation. The diluent is taken out and spread on the solid culture medium for screening ammonia nitrogen degrading bacteria to preliminarily screen out characteristic colonies; the characteristic colonies are excellent strains with high ammonia nitrogen degradation activity.

[0065] (4) Pick a single characteristic colony obtained in step (3) and perform streaking on the LB plate three times to ensure that pure bacteria are obtained as much as possible. The purified single colony is inoculated into LB liquid culture medium and cultured in a constant temperature shaker at 35°C and 180r / min for 3 days. The streaking diagram of the plate is as follows Figure 1 As shown in A.

[0066] Depend on Figure 1 It can be seen that the single bacterial morphology of strain JLSH1 is: the edge of the colony is yellow, the shape is oval, the surface is smooth and opaque, and it has a certain viscosity. The bacterial microscope photo is rod-shaped.

[0067] (5) After repeated plate streaking, the growth performance of the different bacterial strains isolated and their efficiency in degrading ammonia nitrogen in the culture medium were analyzed, and finally a strain JLSH1 with the best ammonia nitrogen degradation effect was obtained.

[0068] At the same time, pick the pure strains on the LB solid plate and observe the cell morphology under a microscope. The results are as follows Figure 1 As shown in B. Before the microscopic examination of the strain, the selected strain needs to be simply stained with ammonium oxalate crystal violet, and then the cell morphology is observed under a microscope.

[0069] The bacterial liquid was inoculated into the MSM basic liquid medium containing 100 mg / L ammonia nitrogen at a 1% inoculation amount, and cultured in a constant temperature shaker at 35°C and 180 r / min for 7 days, and repeated 3 times in parallel. The degradation rate of ammonia nitrogen was measured by phenol sodium hypochlorite colorimetry.

[0070] Example 2 Morphological observation and DNA identification of strain JLSH1

[0071] (1) The selected dominant strains were separated and purified, and then stained with ammonium oxalate crystal violet and observed under a microscope. Morphological observation showed that after 2 days of culture, the colony edge of strain JLSH1 was yellow, oval in shape, smooth and opaque, and had a certain viscosity. The bacterial microscope photo showed a rod-shaped colony morphology as shown in the figure below. Figure 1 A.

[0072] (3) The inventors used a cell disruption method to extract genomic DNA from pure strains, and sequenced the 16S rDNA of strain JLSH1. The nucleotide sequence is shown in SEQ ID NO.1. The measured 16S rDNA sequence was compared by BLAST, indicating that the strain belongs to the genus Pseudomonas. It is currently deposited in the General Biological Center (CGMCC) of the Chinese Academy of Microbiological Culture Collection, Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing, China. The deposit date is November 14, 2024, the deposit number is CGMCC No1.62473, and the recommended classification name is Pseudomonas furukawaii.

[0073] The primers 16S27F and 16S1492R were used for amplification. 5 μl of PCR product was tested by agarose gel electrophoresis. The PCR product was sent to Shanghai Bioengineering Co., Ltd. for sequencing, and the 16S rDNA sequence was submitted to NCBI for BLAST homologous sequence search and comparison.

[0074] The total volume of the PCR reaction system was 20 μL. The order and volume added to each PCR tube were: Prime starMax 10 μL, primers 16S27F and 16S1492R 0.5 μL each, genomic DNA 0.3 μL, and sterile water 8.7 μL.

[0075] The PCR reaction program was as follows: pre-denaturation at 98°C for 5 min, denaturation at 98°C for 15 s, annealing at 54°C for 15 s, extension at 72°C for 50 s, for a total of 31 cycles, and finally full extension at 10°C for 5 min and storage at 20°C.

[0076] Primers:

[0077] 27F:5′-AGAGTTTGATCCTGGCTCAG-3′

[0078] 1492R:5′-GGTTACCTTGTTACGACTT-3′

[0079] The above primers were synthesized by Shanghai Bioengineering Co., Ltd.

[0080] The phylogenetic tree of the degradation bacteria D2 was constructed using the neighbor method. Figure 2 shown.

[0081] Example 3 Optimization of conditions for degradation of ammonia nitrogen by strain JLSH1 in culture medium

[0082] The degradation rate of ammonia nitrogen was measured by phenol sodium hypochlorite colorimetry:

[0083] (1) Prepare 20 ml of LB liquid culture medium, sterilize it in a high pressure steam sterilizer, and then divide it equally into two PA bottles. Pick a single bacterium from the MSM solid culture medium plate and inoculate it into two PA bottles. Then place the PA bottles in a 35°C constant temperature shaking incubator for shaking culture for 2 days.

[0084] (2) Take out the PA bottle containing the bacterial solution and use a pipette to transfer it to the PA bottle containing 10 ml of MSM basal liquid medium after sterilization. While ensuring that other conditions remain unchanged, set different C / N ratios (0-25), inoculation amounts (0-5%), pH (5-9) and temperatures (20-40°C), respectively, and then place it in a constant temperature shaker at 30°C and 180 r / min for shaking culture. Starting from 0h, take 1 ml of sample from each PA bottle every 12h and place the sample in a 2 ml centrifuge tube.

[0085] (3) Place the sample in step (2) into a high-speed centrifuge and set the centrifugation conditions as follows: centrifugation time 2min30s, speed 12000rpm. After centrifugation is completed, use a pipette to first draw 0.005ml of bacterial solution into a 10ml test tube, and then add 0.045ml of pure water for dilution. Then draw 2.5ml of 0.99% phenol and 2ml of 8.5% sodium hypochlorite into the test tube. Then put it into a constant temperature water bath, heat it in a water bath at 95°C for 5min, then take it out and place it in ice water for cooling. After the temperature cools down, use a pipette to draw 0.5ml of bacterial solution into a 48-well plate, set the wavelength to 630nm, measure the absorbance, and at the same time measure the initial ammonia nitrogen content in the culture medium without single bacteria. Repeat for 2 groups, take the average value, and calculate the degradation efficiency of ammonia nitrogen-degrading bacteria. The degradation effect of single-factor experiments under different conditions is as follows: Figure 3-6 shown.

[0086] It can be seen from the experimental results that the ammonia nitrogen degradation efficiency of strain JLSH1 is the highest when the temperature is 30°C, the inoculation amount is 2%, the pH is 6, and the C / N is 7.5.

[0087] Example 4 Degradation efficiency of ammonia nitrogen in culture medium by strain JLSH1 under optimal conditions

[0088] Under the method in Example 3, the C / N ratio was set to 7.5, the inoculation amount was 2%, the pH was 6, and then placed at 30°C and 180 r / min. The degradation rate results are as follows: Figure 7 shown.

[0089] The experiment showed that within 48 hours, as time went on, the degradation efficiency of ammonia nitrogen in MSM-based liquid medium by strain JLSH1 gradually increased, and the initial ammonia nitrogen concentration of MSM-based liquid medium with an initial ammonia nitrogen concentration of 466.04 mg / L could be degraded to 22.90 mg / L, with a degradation rate of up to 95.06%. After 48 hours, there was no significant fluctuation in the degradation rate.

[0090] Example 5 Application of strain JLSH1 in reducing ammonia nitrogen in refinery wastewater

[0091] Take 30ml of refinery wastewater (buffer tank of Jinling Petrochemical Water Purification Area) and put it into a constant temperature shaker at 180r / min for 3 days at a temperature of 30°C, an inoculation amount of 2%, a pH of 6, and a C / N of 7.5. Take a sample every 2 hours, and measure the ammonia nitrogen degradation efficiency according to the method in Example 3, and calculate the corresponding ammonia nitrogen content and degradation efficiency. The results are as follows: Figure 8 shown.

[0092] Experiments have shown that the ammonia nitrogen degradation strain JLSH1 of the present invention has high ammonia nitrogen degradation activity. Under the optimal conditions: C / N ratio of 7.5, inoculation amount of 2%, pH of 6, and 30°C, the strain JLSH1 can degrade the initial ammonia nitrogen concentration of 41.44 mg / L of refinery wastewater to 0.325 mg / L within 8 hours, and the degradation rate is as high as 99.21%.

[0093] As described above, although the present invention has been shown and described with reference to specific preferred embodiments, it should not be construed as limiting the present invention itself. Various changes in form and details may be made without departing from the spirit and scope of the present invention as defined in the appended claims.

Claims

1. An ammonia nitrogen-degrading strain, characterized in that: The strain is Pseudomonas fukagawa furukawaii, named JLSH1, its deposit number is CGMCC No.1.62473, and the sequence of its 16s rDNA fragment is shown in SEQ ID NO.

1.

2. The ammonia nitrogen degrading strain according to claim 1, characterized in that The strain is screened and separated from petroleum refining wastewater; the petroleum refining wastewater is extracted from a biological aeration tank of a petroleum refining workshop.

3. A method for isolating an ammonia nitrogen-degrading strain according to claim 1 or 2, characterized in that: The following steps are involved: (1) Weighing the sewage, adding it to a liquid culture medium for screening ammonia nitrogen-degrading strains for enrichment, and shaking at a constant temperature of 35° C. and 180 rpm for 4-7 days to obtain a culture solution; (2) taking the culture solution obtained in step (1), adding it to another liquid culture medium for screening ammonia nitrogen-degrading strains for subculture, and subculturing for 3-6 times to obtain a culture solution; (3) Take the culture solution obtained in step (2) and prepare 10 -3 , 10 -4 , 10 -5 The diluent is taken out, and the diluent is spread on a solid culture medium for screening ammonia nitrogen degradation strains to separate and preliminarily screen out characteristic colonies; (4) Pick the characteristic colonies obtained in step (3) for plate streaking isolation and purification, and inoculate the purified strains into LB liquid culture medium and culture at 35°C and 180 rpm for 24 h; (5) Analyze the growth performance of the different bacterial strains isolated and their efficiency in degrading ammonia nitrogen in the culture medium, and finally obtain the ammonia nitrogen degrading strain.

4. The separation method according to claim 3, characterized in that The components of the liquid culture medium for screening ammonia nitrogen-degrading strains are: C6H5Na3O7 1g / L; (NH4)2SO4 1g / L; K2HPO4 1.5g / L; KH2PO4 0.5g / L; MgSO4·H2O0.2g / L; NaCl 6g / L; pH 7.2-7.4; The components of the solid culture medium for screening ammonia nitrogen-degrading strains are: C6H5Na3O7 1g / L; (NH4)2SO4 1g / L; K2HPO4 1.5g / L; KH2PO4 0.5g / L; MgSO4·H2O 0.2g / L; NaCl 6g / L; agar powder 15g / L; pH 7.2-7.

4.

5. The separation method according to claim 3, characterized in that The components of the LB liquid culture medium are: 10 g of peptone, 5 g of yeast powder, 10 g of sodium chloride, and the volume is supplemented to 1 L with deionized water.

6. Use of the ammonia nitrogen-degrading strain according to claim 1 or 2 in degrading ammonia nitrogen-contaminated water, characterized in that: The ammonia nitrogen-contaminated water body is refinery wastewater.

7. The use according to claim 6, characterized in that: The conditions for the strain to degrade ammonia-nitrogen polluted water bodies are 25-35° C. and pH 6-8.

8. The use according to claim 6, characterized in that: The inoculation amount of the strain for degrading ammonia-nitrogen polluted water is 0.5-3%.

9. The use according to claim 6, characterized in that: The carbon-nitrogen ratio of the strain in degrading ammonia-nitrogen-contaminated water is 4.5-12.

5.

10. The use according to claim 6, characterized in that: The method for degrading ammonia-nitrogen-contaminated water by the strain comprises the following steps: (1) Activation of strains: strain JLSH1 was inoculated from a slant plate into LB liquid culture medium and cultured at 30-35°C and 180 rpm for 24 h to prepare a bacterial solution; (2) Strain degradation process: The above bacterial solution is inoculated into the refinery wastewater, the pH value is 6-8, the inoculation amount is 0.5-3%, and the culture is carried out at 30-35°C, 150r / min constant temperature shaking for 8h, which can effectively degrade the ammonia nitrogen in the refinery wastewater.

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