A PCR primer set for detecting and differentiating avian reovirus and its application
By designing a multi-fluorescence quantitative PCR method with specific amplification primers and probe combinations, the problems of high limit and low sensitivity of avian reovirus detection in the prior art are solved, and efficient and accurate detection and distinction of different ARV genotypes are achieved.
Patent Information
- Application Number
- CN202510205771.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-25
- Publication Date
- 2025-07-25
- Estimated Expiration
- 2045-02-25
AI Technical Summary
The existing PCR detection methods have high detection limits on avian reoviruses and low sensitivity, making it difficult to effectively distinguish ARV viruses of different genotypes, resulting in inefficient clinical detection.
A specific amplification primer and probe combination was designed to target conserved regions of ARV genotype I, type I subtype, type II and type VI. Multiple fluorescence quantitative PCR method was used to achieve accurate quantitative analysis through fluorescence signal monitoring to avoid cross-reactions.
High sensitivity detection of different genotypes of ARV is achieved, with strong specificity and good repeatability. It can accurately distinguish different genotypes within Ct value ≤40, reduce false positive rates, and improve detection efficiency.