Two-color quantum dot fluorescent ball dual-detection immunochromatographic test strip and its preparation method
By using quantum dot fluorescent balls of different particle sizes and colors and calibration lines in immunochromatographic test strips, the problem of cross-reactivity in multi-index detection was solved, achieving high sensitivity and low false positive and false negative detection results.
CN119688975BActive Publication Date: 2026-07-03THE GBA NAT INST FOR NANOTECHNOLOGY INNOVATION
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- THE GBA NAT INST FOR NANOTECHNOLOGY INNOVATION
- Filing Date
- 2024-12-11
- Publication Date
- 2026-07-03
AI Technical Summary
Technical Problem
In existing immunochromatographic assays, cross-reaction interference exists when multiple indicators are detected, leading to false positive and false negative results.
Method used
Two types of quantum dot fluorescent spheres with different particle sizes and colors were used as fluorescent markers to detect different indicators. The test signals were calibrated using calibration lines. The fluorescence property of quantum dots with uni-excitation and multi-emission was utilized to avoid interference between fluorescent markers.
Benefits of technology
It effectively avoids interference between fluorescent markers during multi-index detection, improves the accuracy and sensitivity of detection, and reduces false positive and false negative results.
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Figure CN119688975B_ABST
Abstract
This invention discloses a dual-color quantum dot fluorescent ball immunochromatographic test strip and its preparation method. The test strip's reaction pad is coated with a first antibody labeled with green quantum dot fluorescent balls and a second antibody labeled with red quantum dot fluorescent balls. The reaction pad is sequentially equipped with a T1 line, a T2 line, a C line, and a calibration line. The T1 line is coated with the first antibody, the T2 line with the second antibody, the C line with the secondary antibody, and the calibration line with yellow water-soluble quantum dots emitting a wavelength of λ3. This invention uses two quantum dot fluorescent balls of different particle sizes and colors as fluorescent markers for two different detection indicators, reducing the influence of cross-reactivity during multi-indicator detection in immunochromatographic assays and avoiding interference between fluorescent markers that occurs during simultaneous detection of multiple indicators.
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