Application of rice sprout or analogue thereof as plant growth regulator

Abstract

The invention discloses application of oryzanol or analogues thereof as a plant growth regulator, and belongs to the technical field of plant growth regulators. In order to screen plant growth regulators from microorganisms, the invention provides a plant growth regulator containing an aqueous solution of the rice sprout or the rice sprout analogue, and the concentration of the rice sprout or the rice sprout analogue in the plant growth regulator is 10-100mg / L. According to the invention, indoor seed germination experiments, indoor and outdoor potting experiments and field experiments are adopted to research the plant growth promoting performance of the rice sprout and the analogues thereof, and the rice sprout and the analogues thereof are found to be capable of promoting growth and development of different food crops or vegetables through different application methods, and have the effects of promoting plant seed germination and plant growth, promoting growth of plants and promoting growth of the plants. The plant growth regulator can be used as a novel plant growth regulator which is derived from microorganisms, efficient, long in lasting period, non-toxic and low in cost to be applied to agricultural production, and has development and utilization value and application prospects in agricultural production.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators, and specifically relates to the application of glutathione or its analogs as plant growth regulators. Background Art

[0002] Plant growth regulators (PGRs) are a class of organic substances with plant hormone activity. Their application can regulate the growth and development of crops and promote the development of cultivation technology (Pan Chen, Yang Yu, Qi Dongliang. Effects of nitrogen fertilizer application patterns on rice dry matter and yield under different irrigation conditions. Journal of Irrigation and Drainage, 2023, 42(2): 73–78.). In recent years, many achievements have been made in the research on plant growth regulators regulating plant resistance to stress. Plant growth regulators have a significant effect on improving plant growth and production capacity by changing the stress resistance of plants. They have a more prominent mitigating effect in many adversities such as drought, cold, flooding and other abiotic stresses (Li Fugen, Wang Yiyan, Liu Shaoren, et al. Pesticide Registration Management Terminology Part 1: Basic Terminology, NY / T 1667.1-2008, Agricultural Industry Standards of the People's Republic of China, 2008.).

[0003] Maltol, also known as maltol, has a chemical name of 3-hydroxy-2-methyl-4-pyrone. It is a compound with plant growth-promoting properties isolated from the metabolites of endophytic bacteria in rapeseed flowers. Maltol is a broad-spectrum flavor enhancer, often added to baked foods, desserts, cosmetics, etc. It is widely used in food, beverages, brewing, cosmetics, pharmaceuticals and other industries (Liu Jing, Xing Jian. 7 Steps in Food Formula Design: Chemical Industry Press, 2012, 84).

[0004] Maltol analogs, also known as ethyl maltol, have a chemical name of 2-ethyl-3-hydroxy-4H-pyranone. Ethyl maltol is a food additive with a small dosage and significant effect. As a flavor modifier and flavor enhancer, it is increasingly widely used. It is a good flavor enhancer for tobacco, food, beverages, flavors, fruit wine, daily cosmetics, etc. It has a significant effect on improving and enhancing the flavor of food, sweetens sweets, and can prolong the storage period of food (Mu Min, Zheng Fuping, Sun Baoguo, et al. Synthesis of maltol and ethyl maltol and their application in food industry [J]. Journal of Chinese Institute of Food Science and Technology, 2006, 6(1):4.).

[0005] However, there is no report on the use of glutamic acid or its analogs as plant growth regulators in the prior art. Since glutamic acid can be isolated from plants (such as tar of beech trees, pine needles, chicory and larch bark, etc.) and microorganisms, it would be of great significance if glutamic acid could be used as a plant growth regulator. Summary of the invention

[0006] In order to screen plant growth regulators from microbial sources, the present invention isolated a compound with plant growth-promoting properties from the metabolites of endophytic bacteria in rapeseed in the early stage, and named it glutamin. After identification of glutamin, it was found to be maltol (3-hydroxy-2-methyl-4-pyrone). The present invention uses indoor seed germination experiments, indoor and outdoor potted experiments and field experiments to study the plant growth-promoting properties of glutamin and its analog ethyl maltol (2-ethyl-3-hydroxy-4H-pyrone), and found that glutamin and its analogs can promote the growth and development of different food crops or vegetables through different application methods. Based on the above research results, the present invention provides the application of glutamin or its analogs as plant growth regulators, and the specific technical scheme is as follows:

[0007] The first object of the present invention is to provide an application of glutamin or its analogues as an effective ingredient of a plant growth regulator in promoting the growth of vegetable seedlings, wherein a glutamin aqueous solution or a glutamin analogue aqueous solution with a concentration of 10 mg / L-100 mg / L is used as a plant growth regulator to spray the vegetable seedlings; the glutamin is maltol, and the glutamin analogue is ethyl maltol; the vegetables include fruit vegetables and leafy vegetables.

[0008] In one embodiment of the present invention, the promoting the growth of vegetable seedlings refers to increasing the plant height, stem thickness, fresh weight and dry weight of fruit vegetable seedlings, or increasing the plant height, root length, leaf area and fresh weight of leafy vegetable seedlings.

[0009] In one embodiment of the present invention, the fruit vegetables include tomatoes, peppers and eggplants, and the leafy vegetables include Chinese cabbage, spinach, lettuce, romaine lettuce and rapeseed.

[0010] The second object of the present invention is to provide an application of glutamin or its analogues as an effective ingredient of a plant growth regulator in promoting the germination of rice seeds, wherein the application uses an aqueous solution of glutamin or an aqueous solution of glutamin analogues with a concentration of 30 mg / L-90 mg / L as a plant growth regulator to soak rice seeds; the glutamin is maltol, and the glutamin analogue is ethyl maltol.

[0011] In one embodiment of the present invention, the seed soaking is performed at room temperature for 48 hours.

[0012] In one embodiment of the present invention, the promoting rice seed germination refers to increasing the germination rate, sprout length and root length of the rice seeds.

[0013] Preferably, the concentration of the aqueous solution of glutathione is 50 mg / L, and the concentration of the aqueous solution of glutathione analogs is 70 mg / L.

[0014] The third object of the present invention is to provide an application of glutamin or its analogues as an effective ingredient of a plant growth regulator in improving the cold resistance of rice seeds, wherein the application uses a glutamin aqueous solution or a glutamin analogue aqueous solution with a concentration of 30 mg / L-90 mg / L as a plant growth regulator to soak rice seeds to promote the germination of rice seeds under low temperature conditions, wherein the low temperature is 15-18°C; the glutamin is maltol, and the glutamin analogue is ethyl maltol.

[0015] In one embodiment of the present invention, the seed soaking is performed at room temperature for 48 hours.

[0016] In one embodiment of the present invention, the promoting the germination of rice seeds under low temperature conditions refers to increasing the germination rate, sprout length and root length of rice seeds under low temperature conditions.

[0017] Preferably, the concentration of the aqueous solution of glutathione is 50 mg / L, and the concentration of the aqueous solution of glutathione analogs is 70 mg / L.

[0018] The fourth object of the present invention is to provide an application of glutenin as an effective ingredient of a plant growth regulator in promoting rice development, wherein the application uses a glutenin aqueous solution with a concentration of 30-50 mg / L as a plant growth regulator to soak rice seeds to promote the growth and tillering of rice at different growth stages; the glutenin is maltol.

[0019] In one embodiment of the present invention, the seed soaking is performed at room temperature for 48 hours.

[0020] In one embodiment of the present invention, promoting the growth and tillering of rice at different growth stages refers to increasing the plant height, root length, fresh weight and dry weight of rice seedlings, and increasing the plant height and tiller number at the tillering stage and heading stage.

[0021] The fifth object of the present invention is to provide an application of glutenin as an effective ingredient of a plant growth regulator in improving the cold resistance of rice seedlings, wherein the application uses a glutenin aqueous solution with a concentration of 30-50 mg / L as a plant growth regulator to soak rice seeds to promote the growth of rice seedlings under low temperature conditions, wherein the low temperature is 15-18°C; the glutenin is maltol.

[0022] In one embodiment of the present invention, the seed soaking is performed at room temperature for 48 hours.

[0023] In one embodiment of the present invention, the promoting the growth of rice seedlings under low temperature conditions refers to increasing the plant height, root length, fresh weight and dry weight of the rice seedlings under low temperature conditions.

[0024] The sixth object of the present invention is to provide an application of glutenin as an effective ingredient of a plant growth regulator in improving rice yield, wherein the application uses an aqueous solution of glutenin with a concentration of 30-50 mg / L as a plant growth regulator to soak rice seeds; the glutenin is maltol.

[0025] In one embodiment of the present invention, the seed soaking is performed at room temperature for 48 hours.

[0026] In one embodiment of the present invention, increasing rice yield refers to increasing the number of effective rice panicles, yield per plant, panicle length, number of grains per panicle, number of filled grains, fruit setting rate, 1000-grain weight, grain length and grain width.

[0027] The seventh objective of the present invention is to provide an application of glutenin as an effective ingredient of a plant growth regulator in promoting the germination of grain crop seeds, wherein the application uses a seed coating agent with a glutenin concentration of 5-10 mg / L as a plant growth regulator to coat grain crop seeds; the glutenin is maltol.

[0028] In one embodiment of the present invention, the food crops include rice, wheat, corn and soybean.

[0029] In one embodiment of the present invention, the promoting the germination of grain crop seeds refers to increasing the germination rate of grain crop seeds.

[0030] The eighth object of the present invention is to provide an application of maltol as an effective ingredient of a plant growth regulator in improving the cold resistance of grain crop seeds, wherein the application uses a seed coating agent with a maltol concentration of 5-10 mg / L as a plant growth regulator to coat grain crop seeds to promote the germination of grain crop seeds under low temperature conditions, wherein the low temperature is 15-18°C; the maltol is maltol.

[0031] In one embodiment of the present invention, the food crops include rice, wheat, corn and soybean.

[0032] In one embodiment of the present invention, the promoting the germination of grain crop seeds under low temperature conditions refers to increasing the germination rate of grain crop seeds under low temperature conditions.

[0033] The ninth objective of the present invention is to provide an application of glutenin as an effective ingredient of a plant growth regulator in promoting the growth of seedlings of grain crops, wherein the application uses a seed coating agent with a glutenin concentration of 5-10 mg / L as a plant growth regulator to coat grain crop seeds; the glutenin is maltol.

[0034] In one embodiment of the present invention, the food crops include rice, wheat, corn and soybean.

[0035] In one embodiment of the present invention, the promoting the growth of food crop seedlings refers to increasing the plant height and root length of the food crop seedlings.

[0036] The tenth object of the present invention is to provide an application of maltol as an effective ingredient of a plant growth regulator in improving the cold resistance of grain crop seedlings, wherein the application uses a seed coating agent with a maltol concentration of 5-10 mg / L as a plant growth regulator to coat grain crop seeds to promote the growth of grain crop seedlings under low temperature conditions; the maltol is maltol.

[0037] In one embodiment of the present invention, the food crops include rice, wheat, corn and soybean.

[0038] In one embodiment of the present invention, the promoting the growth of food crop seedlings under low temperature conditions refers to increasing the plant height and root length of the food crop seedlings under low temperature conditions.

[0039] Beneficial effects of the present invention:

[0040] The present invention finds that an aqueous solution of maltol or its analog (ethyl maltol) can be used as a plant growth regulator to regulate the growth and development of food crops and vegetables, and the specific effects are as follows:

[0041] The present invention finds through indoor potted plant experiments that spraying vegetable seedlings with an aqueous solution of glutamic acid or its analogs can promote the growth of vegetable seedlings, specifically by increasing the plant height, stem thickness, fresh weight and dry weight of fruit vegetable seedlings, or increasing the plant height, root length, leaf area and fresh weight of leafy vegetable seedlings.

[0042] The present invention finds through indoor seed germination experiments that soaking rice seeds in an aqueous solution of glutathione or its analogues can promote the germination of rice seeds under normal temperature and low temperature stress conditions and improve the cold resistance of rice seeds, which is specifically manifested in improving the germination rate, sprout length and root length of rice seeds under normal temperature or low temperature stress conditions.

[0043] The present invention finds through indoor potted plant experiments that soaking rice seeds with an aqueous solution of glutenin can promote the growth of rice seedlings under normal temperature and low temperature stress conditions and improve the cold resistance of rice seedlings, which is specifically manifested in improving the plant height, root length, fresh weight and dry weight of rice seedlings under normal temperature or low temperature stress conditions.

[0044] The present invention finds through outdoor pot plant experiments and field experiments that soaking rice seeds with an aqueous solution of glutenin can promote the growth and tillering of rice at different growth stages and improve rice yield, which is specifically manifested in increasing the plant height of rice in the seedling stage, tillering stage and heading stage, increasing the number of tillers of rice in the tillering stage and heading stage, and increasing the number of effective rice panicles, single plant yield, panicle length, number of panicles, number of filled grains, fruit setting rate, 1,000-grain weight, grain length and grain width.

[0045] The present invention finds through indoor potted plant experiments that soaking the seeds of grain crops with a coating agent containing an aqueous solution of malt extract as an effective ingredient can promote the germination of grain crop seeds under normal temperature and low temperature stress conditions, promote the growth of grain crop seedlings under normal temperature and low temperature stress conditions, and improve the cold resistance of grain crop seeds and seedlings, which is specifically manifested in improving the germination rate of grain crop seeds and the plant height and root length of seedlings under normal temperature or low temperature stress conditions.

[0046] In summary, the aqueous solution of glutamic acid or its analogues provided by the present invention has the effects of promoting plant seed germination and plant growth, increasing plant yield and resistance to low temperature stress, and can be used as a novel plant growth regulator with high efficiency, long-lasting effect, non-toxicity and low cost from microorganisms for agricultural production, and has development and utilization value and application prospects in agricultural production. The present invention provides a research basis for its next step of registration application, batch production and large-scale agricultural use, and has important practical significance. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 The figure shows the effect of soaking rice seedlings in 50 mg / L glutamic acid aqueous solution on the growth state of rice seedlings in an indoor normal temperature / low temperature pot experiment; wherein, NT CK is the control group at normal temperature, NT Tr is the treatment group at normal temperature, LT CK is the control group at low temperature, and LTTr is the treatment group at low temperature;

[0048] Figure 2 This is the result of soaking rice seedlings in 50 mg / L glutamic acid aqueous solution on the growth status of rice seedlings at different growth stages in an outdoor pot experiment; CK is the control group and Tr is the treatment group;

[0049] Figure 3 This is a graph showing the effect of soaking rice seedlings in a 50 mg / L glutamic acid aqueous solution on the growth status of rice seedlings at different growth stages in a field experiment; Figure 3 A in the figure represents the growth state of rice seedlings. Figure 3 B in the figure represents the growth state of rice during the tillering stage. Figure 3 C in the figure represents the growth state of rice at the heading stage. Figure 3 D in the figure represents the growth state of rice at maturity; CK represents the control group, and Tr represents the treatment group;

[0050] Figure 4 This is a graph showing the effect of coating rice, wheat, corn, and soybean seeds with a 10 mg / L effective concentration of glutamic acid on the growth status of rice, wheat, corn, and soybean seedlings in an indoor pot experiment at room temperature; Figure 4 A in the figure represents the growth state of rice seedlings. Figure 4 B in the figure represents the growth state of wheat seedlings. Figure 4 C in the figure represents the growth state of corn seedlings. Figure 4 D in the figure represents the growth state of soybean seedlings; CK represents the control group, and Tr represents the treatment group;

[0051] Figure 5 This is a graph showing the effect of coating rice, wheat, corn, and soybean seeds with a 10 mg / L effective concentration of glutamic acid on the growth status of rice, wheat, corn, and soybean seedlings in an indoor low-temperature pot experiment; Figure 5 A in the figure represents the growth state of rice seedlings. Figure 5 B in the figure represents the growth state of wheat seedlings. Figure 5 C in the figure represents the growth state of corn seedlings. Figure 5 D in the figure represents the growth state of soybean seedlings; CK is the control group, and Tr is the treatment group. DETAILED DESCRIPTION

[0052] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific implementation methods and the accompanying drawings of the specification. The embodiments described in the present invention are not all embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0053] The experimental methods used in the following examples are all conventional methods unless otherwise specified, and the materials, reagents and instruments used are all conventional materials, reagents and instruments in the art unless otherwise specified, and can be obtained by those skilled in the art through commercial channels.

[0054] The glutamin of the present invention is also known as maltol, and its chemical name is 3-hydroxy-2-methyl-4-pyrone, and its chemical structure is shown in Formula I; the glutamin analog of the present invention is also known as ethyl maltol, and its chemical name is 2-ethyl-3-hydroxy-4H-pyrone, and its chemical structure is shown in Formula II;

[0055]

[0056] Example 1: Application of glutenin spraying in promoting the growth of fruits and leafy vegetables

[0057] The application described in this embodiment is to use 10 mg / L and 100 mg / L glutamic acid aqueous solution to spray tomato, pepper, eggplant, Chinese cabbage, spinach, lettuce, lettuce, and rapeseed seedlings. The specific steps are as follows:

[0058] (1) Select tomato, pepper, eggplant, Chinese cabbage, spinach, lettuce, romaine lettuce, and rapeseed seeds with full seeds and uniform size, and soak them at 28°C for 6 hours after surface disinfection.

[0059] (2) The seeds with cracks after soaking were sown in flower pots with an inner diameter of 12 cm and a height of 10 cm. Each pot was filled with 200 g of planting soil and 5 germinated seeds were planted in each pot. A blank control group (CK) and a treatment group (Tr) were set for each crop. Each treatment group was set with 15 parallels and cultured at 28°C with a photoperiod of 12L / 12D.

[0060] (3) Weigh 10 mg and 100 mg of glutamic acid respectively, dissolve in deionized water, and adjust the volume to 1 L. On the 5th day after sowing in step (2), thin the seedlings, that is, keep one seedling with good and consistent growth in each pot, spray the Tr seedlings with the glutamic acid aqueous solution, and spray the CK seedlings with deionized water. After the above treatment, continue to cultivate for 14 days, and count the plant height, stem diameter, root length, leaf area, fresh weight and dry weight of the seedlings. The experiment was repeated 3 times, and the results are shown in Tables 1 and 2.

[0061] Table 1 Statistical results of the effect of spraying 100 mg / L glutamic acid aqueous solution on the growth of fruit vegetable seedlings

[0062]

[0063]

[0064] Table 2 Statistical results of the effect of spraying 100 mg / L glutamic acid aqueous solution on the growth of leafy vegetable seedlings

[0065]

[0066] As shown in Table 1, spraying 100 mg / L glutamic acid aqueous solution can promote the growth of tomato, pepper and eggplant seedlings. Compared with the control, the plant height, stem diameter, fresh weight and dry weight of tomato increased by 25.56%, 8.1%, 10.29% and 11.58% respectively, the plant height, stem diameter, fresh weight and dry weight of pepper increased by 18.16%, 6.97%, 23.7% and 14.7% respectively, and the plant height, stem diameter, fresh weight and dry weight of eggplant increased by 38.78%, 4.74%, 25.29% and 34.06% respectively. As shown in Table 2, spraying 100 mg / L glutamin aqueous solution can promote the growth of Chinese cabbage, spinach, lettuce, lettuce and rapeseed seedlings. Compared with the control, the plant height, root length, leaf area and fresh weight of Chinese cabbage increased by 36.05%, 19.82%, 19.65% and 33.41%, respectively, and the plant height, root length, leaf area and fresh weight of spinach increased by 24.86%, 21.82%, 23.28% and 17.0 3%, the plant height, root length, leaf area and fresh weight of rapeseed increased by 11.35%, 12.57%, 13.28% and 14.2% respectively, the plant height, root length, leaf area and fresh weight of lettuce increased by 21.65%, 15.82%, 17.41% and 25.33% respectively, and the plant height, root length, leaf area and fresh weight of rapeseed increased by 38.22%, 18.28%, 33.26% and 39.33% respectively. Similar effects can be obtained by testing with 10mg / L glutamic acid aqueous solution, but the effect is slightly weaker than that of 100mg / L concentration.

[0067] Example 2: Application of glutathione analogs in promoting the growth of fruits and leafy vegetables

[0068] The application described in this embodiment is to spray tomato, pepper, eggplant, Chinese cabbage, spinach, lettuce, lettuce, and rapeseed seedlings with an aqueous solution of 10 mg / L and 100 mg / L of glutathione analogs. The specific steps are as follows:

[0069] (1) Select tomato, pepper, eggplant, Chinese cabbage, spinach, romaine lettuce, and rapeseed seeds with full seeds and uniform size, and soak them at 28°C for 8 hours after surface disinfection.

[0070] (2) The seeds with cracks after soaking were sown in flower pots with an inner diameter of 12 cm and a height of 10 cm. Each pot was filled with 200 g of planting soil and 5 germinated seeds were planted in each pot. A blank control group (CK) and a treatment group (Tr) were set for each crop. Each treatment group was set with 15 parallels and cultured at 28°C with a photoperiod of 12L / 12D.

[0071] (4) Weigh 10 mg and 100 mg of glutamic acid analogs respectively, dissolve them in deionized water, and adjust the volume to 1 L. On the 5th day after sowing in step (2), the seedlings were treated for seedling establishment, that is, one seedling with good and consistent growth was retained in each pot, and the Tr seedlings were sprayed with the above-mentioned glutamic acid analog aqueous solution, and the CK seedlings were sprayed with deionized water. After the above treatment, the seedlings were cultured for 14 days, and the plant height, stem diameter, root length, leaf area, fresh weight and dry weight of the seedlings were counted. The experiment was repeated 3 times, and the results are shown in Tables 3 and 4.

[0072] Table 3 Statistical results of the effect of spraying 100 mg / L glutamic acid analogue aqueous solution on the growth of fruit vegetable seedlings

[0073]

[0074] Table 4 Statistical results of the effect of spraying 100 mg / L glutamic acid analogue aqueous solution on the growth of leafy vegetable seedlings

[0075]

[0076] As shown in Table 3, spraying 100 mg / L glutamic acid analogue aqueous solution can promote the growth of tomato, pepper and eggplant seedlings. Compared with the control, the plant height, stem diameter, fresh weight and dry weight of tomato increased by 10.83%, 4.88%, 7.29% and 11.11%, respectively; the plant height, stem diameter, fresh weight and dry weight of pepper increased by 8.36%, 3.21%, 11.86% and 7.03%, respectively; the plant height, stem diameter, fresh weight and dry weight of eggplant increased by 32.53%, 10.55%, 13.69% and 17.53%, respectively. As shown in Table 4, spraying the aqueous solution of glutamic acid analogues can promote the growth of Chinese cabbage, spinach, lettuce, lettuce and rapeseed seedlings. Compared with the control, the plant height, root length, fresh weight and dry weight of Chinese cabbage increased by 21.91%, 15.97%, 12.83% and 17.91%, respectively, and the plant height, root length, leaf area and fresh weight of spinach increased by 7.23%, 10.47%, 11.15% and 15.3 6%, the plant height, root length, leaf area and fresh weight of rapeseed increased by 7.05%, 4.23%, 1.97% and 16.35% respectively, the plant height, root length, leaf area and fresh weight of lettuce increased by 12.27%, 9.39%, 7.9% and 16.53% respectively, and the plant height, root length, leaf area and fresh weight of rapeseed increased by 14.01%, 11.45%, 13.18% and 21.43% respectively. Similar effects can be obtained by testing with 10mg / L aqueous solution of glutamic acid analogs, but the effect is slightly weaker than that of 100mg / L concentration.

[0077] Example 3: Application of glutenin to promote rice seed germination in a laboratory under normal or low temperature conditions by soaking seeds

[0078] The application described in this embodiment is to soak rice seeds in aqueous solutions of glutamic acid with different concentrations in the range of 30 mg / L-90 mg / L, soak them at room temperature for 48 hours, and then place the soaked rice seeds in a laboratory under normal temperature or low temperature stress environment for germination experiment. The specific steps are as follows:

[0079] (1) Weigh 100 mg of glutamic acid, dissolve it in deionized water, and make up to 1 L to prepare a 100 mg / L stock solution.

[0080] (2) Take different volumes of the mother solution in step (1), add deionized water according to the proportion, and dilute to aqueous solutions of glutathione with different concentrations between 30 mg / L and 90 mg / L. The concentrations are shown in Table 5.

[0081] (3) Select Longjing 31 rice seeds with full and uniform grain size, disinfect them with 75% alcohol by volume for 3 min, rinse them with distilled water for 3 times, and soak them in aqueous solutions of glutamic acid with different concentration gradients for 48 h.

[0082] (4) The soaked rice seeds were transferred to a 9 cm diameter petri dish covered with sterilized filter paper. Ten rice seeds were placed in each petri dish. 5 mL of sterilized deionized water was added to each petri dish. The process was repeated 3 times. The petri dishes were placed in room temperature (28°C) and low temperature (15°C) in the dark for 4 days. The germination of the rice seeds was observed and the root length and sprout length of the rice were counted. The results are shown in Table 5.

[0083] Table 5 Statistical results of the effects of soaking rice seeds in aqueous solutions of different concentrations of glutamic acid on germination at room temperature / low temperature

[0084]

[0085] From the data shown in Table 5, it can be seen that soaking rice seeds with different concentrations of glutamic acid aqueous solution can promote rice seed germination, improve rice seed germination rate, and improve the cold resistance of rice seeds. In the normal temperature germination experiment, when the concentration of glutamic acid aqueous solution is 50 mg / L, the germination effect is the best. Compared with the normal temperature control, the germination rate, root length, and bud length are increased by 5.45%, 10.00%, and 11.73% respectively; in the low temperature stress germination experiment, when the concentration of glutamic acid aqueous solution is 50 mg / L, the germination effect is the best. Compared with the low temperature control, the germination rate, root length, and bud length are increased by 6.08%, 12.88%, and 17.09% respectively.

[0086] Example 4: Application of glutathione analogs to promote rice seed germination in a laboratory under normal or low temperature conditions by soaking seeds

[0087] The application described in this embodiment is to soak rice seeds in aqueous solutions of glutathione analogs at different concentrations ranging from 30 mg / L to 90 mg / L, soak them at room temperature for 48 hours, and then place the soaked rice seeds in a laboratory at normal temperature or low temperature stress environment for germination experiment. The specific steps are as follows:

[0088] (1) Weigh 100 mg of glutathione analog, dissolve it in deionized water, and dilute to 1 L to prepare a 100 mg / L stock solution.

[0089] (2) Take different volumes of the mother solution in step (1), add deionized water according to the proportion, and dilute to aqueous solutions of glutathione analogs with different concentrations between 30 mg / L and 90 mg / L. The concentrations are shown in Table 6.

[0090] (3) Select Longjing 31 rice seeds with full and uniform grain size, disinfect them with 75% alcohol by volume for 3 min, rinse them with distilled water for 3 times, and soak them in aqueous solutions of glutathione analogs with different concentration gradients for 48 h.

[0091] (4) The soaked rice seeds were transferred to a 9 cm diameter petri dish covered with sterilized filter paper. Ten rice seeds were placed in each petri dish. 5 mL of sterilized deionized water was added to each petri dish. The process was repeated 3 times. The petri dishes were placed in room temperature (28°C) and low temperature (15°C) in the dark for 4 days. The germination of the rice seeds was observed and the root length and sprout length of the rice were counted. The results are shown in Table 6.

[0092] Table 6 Statistical results of the effects of soaking rice seeds in aqueous solutions of different concentrations of glutathione analogs at room temperature / low temperature on the germination of rice seeds

[0093]

[0094] From the data shown in Table 6, it can be seen that soaking rice seeds with different concentrations of glutamin analog aqueous solution can promote rice seed germination, improve rice seed germination rate, and improve the cold resistance of rice seeds. In the normal temperature germination experiment, the germination promotion effect is best when the concentration of glutamin analog aqueous solution is 70 mg / L. Compared with the normal temperature control, the germination rate, root length, and bud length are increased by 5.20%, 9.95%, and 9.42%, respectively; in the low temperature stress germination experiment, the germination promotion effect is best when the concentration of glutamin analog aqueous solution is 70 mg / L. Compared with the low temperature control, the germination rate, root length, and bud length are increased by 4.89%, 9.63%, and 19.59%, respectively.

[0095] Example 5: Application of glutamyl extract in promoting the growth of rice seedlings in indoor normal temperature / low temperature potted experiments by seed soaking

[0096] The application described in this embodiment is to soak Longjing 31 rice seeds in aqueous solutions of 30 mg / L and 50 mg / L glutamic acid respectively, soak them at room temperature for 48 hours, and then sow the soaked rice seeds in pots for indoor rice pot experiments. The specific steps are as follows:

[0097] (1) Weigh 30 mg and 50 mg of glutamic acid respectively, dissolve in deionized water, and dilute to 1 L to prepare 30 mg / L and 50 mg / L glutamic acid aqueous solutions.

[0098] (2) Select Longjing 31 rice seeds with full and uniform grain size, disinfect them with 75% alcohol by volume for 3 min, rinse them with distilled water for 3 times, and then soak them in 30 mg / L and 50 mg / L glutamic acid aqueous solutions for 48 h, respectively. The blank control was soaked in deionized water.

[0099] (3) The soaked Longjing 31 rice seeds were used in a pot experiment. Specifically, the seeds with white heads leaking out after soaking were transplanted into a flower pot with an inner diameter of 12 cm and a height of 10 cm (each pot was filled with 200 g of planting soil). Five germinated seeds were sown in each pot. Each group of treatments had 15 parallels and were cultured at room temperature (28°C) and low temperature (15°C), respectively. The photoperiod was 12L / 12D and the culture was carried out for 14 days. The plant height, root length, fresh weight and dry weight of the rice seedlings were counted. The experiment was repeated 3 times. The results are shown in Tables 7 and Figure 1 shown.

[0100] Table 7 Statistical results of the effect of 50 mg / L glutamic acid aqueous solution on the growth of rice seedlings in indoor normal temperature / low temperature pot experiments

[0101]

[0102] As can be seen from Table 7, soaking rice seeds in a 50 mg / L glutamin aqueous solution can promote the growth of rice seedlings at normal and low temperatures, and enhance the cold resistance of rice seedlings under low temperature stress. In the indoor normal temperature potted experiment, compared with the control group not soaking with glutamin aqueous solution, soaking rice seeds in a 50 mg / L glutamin aqueous solution can increase the plant height, root length, fresh weight and dry weight of rice seedlings by 30.30%, 6.96%, 29.33% and 14.39%, respectively; in the indoor low temperature potted experiment, compared with the control group not soaking with glutamin aqueous solution, soaking rice seeds in a 50 mg / L glutamin aqueous solution can increase the plant height, root length, fresh weight and dry weight of rice seedlings by 17.26%, 16.83%, 25.29% and 13.30%, respectively. Similar effects were obtained by testing with a 30 mg / L aqueous solution of glutathione, but the effect was slightly weaker than that of a 50 mg / L concentration.

[0103] Example 6: Application of glutamyl ester in seed soaking to increase rice plant height, tillering number and yield in outdoor potted plant experiments

[0104] The application described in this embodiment is to soak Longjing 31 rice seeds in aqueous solutions of 30 mg / L and 50 mg / L glutamic acid respectively, soak them at room temperature for 48 hours, and then sow the soaked rice seeds in pots for outdoor rice pot experiments. The specific steps are as follows:

[0105] (1) Weigh 30 mg and 50 mg of glutamic acid respectively, dissolve in deionized water, and dilute to 1 L to prepare 30 mg / L and 50 mg / L glutamic acid aqueous solutions.

[0106] (2) Select Longjing 31 rice seeds with full and uniform grain size, disinfect them with 75% alcohol by volume for 3 min, rinse them with distilled water for 3 times, and then soak them in 30 mg / L and 50 mg / L glutamic acid aqueous solutions for 48 h, respectively. The blank control was soaked in deionized water.

[0107] (3) The soaked Longjing 31 rice seeds were used in a potted experiment. Specifically, the seeds with white heads after soaking were transplanted into pots with an inner diameter of 25 cm and a height of 17.5 cm (each pot was filled with 4.5 kg of planting soil, 0.74 g of ammonium sulfate, 1.47 g of urea, and 0.49 g of diammonium phosphate). Fifteen germinated seeds were sown in each pot. When the seedlings grew the first true leaf, the seedlings were established, that is, one seedling with good and consistent growth was retained in each pot. Each group of treatments was set up with 25 parallels, and a representative pot of rice was selected for photographing and recording at each growth cycle.

[0108] The plant height and tiller number of rice were counted at the tillering stage and heading stage, and the results are shown in Table 8. The rice plants were photographed at the tillering stage, heading stage and maturity stage. Figure 2 shown.

[0109] Table 8 Statistical results of the effect of 50 mg / L glutamic acid aqueous solution on rice plant height and tillering number in outdoor pot experiment

[0110]

[0111] As can be seen from Table 8, soaking rice seeds with 50 mg / L glutamic acid aqueous solution before sowing can promote the growth and tillering of rice. Compared with the control, the rice plant height and tiller number at the tillering stage increased by 6.67% and 15.19% respectively; the rice plant height and tiller number at the heading stage increased by 0.76% and 19.12% respectively. The experiment with 30 mg / L glutamic acid aqueous solution can also obtain similar effects, but the effect is slightly weaker than that of the 50 mg / L glutamic acid aqueous solution treatment group.

[0112] The number of effective ears was counted during the rice maturity period, and indoor seed testing was carried out after harvest. The number of effective ears, yield per plant, ear length, number of grains per ear, number of filled grains, fruit setting rate, 1000-grain weight, grain length, and grain width were investigated. The results are shown in Table 9.

[0113] Table 9 Statistical results of the effect of soaking seeds with 50 mg / L glutamic acid aqueous solution on rice yield in outdoor pot experiment

[0114]

[0115]

[0116] As can be seen from Table 9, soaking rice seeds with 50 mg / L glutamin aqueous solution before sowing can increase the number of effective panicles of rice and improve rice yield. Compared with the control, the number of effective panicles increased by 31.64%, and the rice yield per plant, panicle length, number of panicles, number of filled grains, fruiting rate, and 1,000-grain weight increased by 36.08%, 9.09%, 6.20%, 14.16%, 7.49%, and 7.27%, respectively. Similar effects can be obtained by testing with 30 mg / L glutamin aqueous solution, but the effect is slightly weaker than that of the 50 mg / L glutamin aqueous solution treatment group.

[0117] Example 7: Application of glutamyl in soaking rice seeds to improve rice plant height, tillering number and yield in field experiments

[0118] The application described in this embodiment is to soak Longjing 31 rice seeds in a 50 mg / L glutamic acid aqueous solution at room temperature for 48 hours, and then directly sow the soaked rice seeds in the field for rice field experiments. The specific steps are as follows:

[0119] (1) Weigh 50 mg of glutamic acid, dissolve it in deionized water, and make up to 1 L to prepare a 50 mg / L glutamic acid aqueous solution.

[0120] (2) Select Longjing 31 rice seeds with full and uniform grain size, disinfect them with 75% alcohol by volume for 3 min, rinse them with distilled water for 3 times, and soak them in 50 mg / L glutamic acid aqueous solution for 48 h. For the blank control, soak them in deionized water.

[0121] (3) The soaked Longjing 31 rice seeds were used in field experiments. Specifically, the seeds with white heads after soaking were sown manually by direct seeding (hole sowing) in an area of ​​80 m 2 , row spacing 30cm, hole spacing 30cm, sowing rate 5-10 seeds per hole, fertilizer rate urea 150kg / hm 2 , Potassium oxide 70kg / hm 2 , Phosphorus pentoxide 70kg / hm2 .

[0122] The plant height and tiller number of rice were counted at the seedling stage, tillering stage and heading stage, and the results are shown in Table 10. The rice plants were photographed at the seedling stage, tillering stage, heading stage and maturity stage, as shown in Table 10. Figure 3 shown.

[0123] Table 10 Statistical results of the effect of glutamic acid aqueous solution soaking on plant height and tiller number of rice at different growth stages in the field

[0124]

[0125]

[0126] It can be seen from Table 10 that after the rice seeds were soaked in a 50 mg / L glutamic acid aqueous solution and then directly seeded in the field, the growth of rice at different stages was significantly promoted, and the number of tillers was also significantly increased. Compared with the control, the rice plant height at the seedling stage increased by 14.66%; the rice plant height and the number of tillers at the tillering stage increased by 10.51% and 49.81% respectively; the rice plant height and the number of tillers at the heading stage increased by 3.63% and 28.43% respectively.

[0127] During the rice maturity period, the number of effective panicles, number of grains per panicle, number of filled grains, fruit setting rate, 1000-grain weight and yield per mu were counted. The results are shown in Table 11.

[0128] Table 11 Effect of soaking seeds in glutinin aqueous solution on rice yield in the field

[0129]

[0130] It can be seen from Table 11 that after the rice seeds were soaked in a 50 mg / L glutinin aqueous solution and then directly seeded in the field, the rice yield was significantly improved. Compared with the control, the number of effective ears per square meter, the number of grains per ear, the number of filled grains, the thousand-grain weight, and the yield per mu increased by 18.95%, 20.94%, 17.63%, 6.54%, and 19.40% respectively.

[0131] Example 8: Application of glutenin in a coating form to promote seed germination and seedling development of rice, wheat, corn and soybean in normal temperature / low temperature pot experiments

[0132] The application described in this embodiment is to mix the aqueous solution of glutamin with a universal coating agent, and use the obtained mixed solution (the effective concentration of glutamin is 5 mg / L or 10 mg / L) to coat rice, wheat, corn, and soybean seeds, and then sow the coated rice, wheat, corn, and soybean seeds in pots and place them in a laboratory under normal temperature or low temperature stress environment. The specific steps are as follows:

[0133] (1) Weigh 100 mg of glutamic acid, dissolve it in deionized water, and dilute to 1 L to prepare a stock solution with a concentration of 100 mg / L.

[0134] (2) The mother liquor obtained in step (1) was mixed with a coating agent (active ingredients: 2.35% of ipiconazole and 1.88% of metalaxyl) at a volume ratio of 1:19 and 1:9, respectively, and then 1 mL of the mixture was added per 500 g of seeds to coat rice, wheat, corn, and soybean seeds, respectively, and dried in a shaded place, which was the treatment group (Tr). The seeds coated with only the coating agent were the control group (CK).

[0135] (3) The coated rice, wheat, corn and soybean seeds were used in pot experiments. Specifically, the coated seeds were sown in flower pots with an inner diameter of 12 cm and a height of 10 cm. Each pot was filled with 200 g of planting soil. Five seeds were planted in each rice pot, and three seeds were planted in each wheat, corn and soybean pots. Each group of treatments had 15 parallels, and they were cultured at room temperature (28°C) and low temperature (15°C), respectively. The photoperiod was 12L / 12D. The seedlings were cultured for 14 days at room temperature and for 21 days at low temperature. The seed germination rate was counted at the early stage of germination. For the pots at room temperature, corn and soybean seedlings were established on the 4th day after sowing, and for the pots at low temperature, corn and soybean seedlings were established on the 7th day after sowing, that is, one seedling with good and consistent growth was retained in each pot. After the cultivation, the plant height and root length of the seedlings were counted. The experiment was repeated 3 times. The results are shown in Table 12, Figure 4 and Figure 5 shown.

[0136] Table 1 Statistical results of the effects of 10 mg / L glutamic acid coating on crop seed germination and seedling development in normal temperature / low temperature pot experiments

[0137]

[0138] As shown in Table 12, compared with the control group, after the seeds were coated with 10 mg / L glutinin, the germination rates of rice, wheat, corn and soybean were improved, and the low temperature stress of seeds and seedlings was alleviated, and the growth of seedlings was promoted. Under normal temperature culture conditions, compared with the control group, the germination rate, plant height and root length of rice, wheat, corn and soybean were increased by 5.45%, 14.08%, 13.79%, 1.72%, 28.49%, 28.72%, 8.69%, 13.07%, 26.24%, 4.34%, 17.29% and 23.36% respectively by using the coating agent containing glutenin aqueous solution to coat the seeds; under low temperature culture conditions, compared with the control group, the germination rate, plant height and root length of rice, wheat, corn and soybean were increased by 6.08%, 23.24%, 11.11%, 1.89%, 15.52%, 17.49%, 16.67%, 13.66%, 22.63%, 29.4%, 13.31% and 47.26% respectively by using the coating agent containing glutenin aqueous solution to coat the seeds. Similar effects were obtained by testing seed coating agents containing 5 mg / L glutamic acid, but the effect was slightly weaker than that of the 10 mg / L glutamic acid coating treatment group.

[0139] Although the present invention has been disclosed as above in the form of preferred embodiments, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be based on the definition of the claims.

Claims

1. The use of glutathione or its analogues as an active ingredient of a plant growth regulator in promoting the growth of vegetable seedlings, characterized in that: The application uses a 10mg / L-100mg / L aqueous solution of glutamin or a glutamin analogue aqueous solution as a plant growth regulator to spray vegetable seedlings; the glutamin is maltol, and the glutamin analogue is ethyl maltol; the vegetables include fruit vegetables and leafy vegetables.

2. Use of glutamic acid or its analogues as an active ingredient of a plant growth regulator in promoting rice seed germination, characterized in that: The invention uses a 30mg / L-90mg / L aqueous solution of glutamin or a glutamin analogue aqueous solution as a plant growth regulator to soak rice seeds; the glutamin is maltol, and the glutamin analogue is ethyl maltol.

3. Use of glutamic acid or its analogues as an effective ingredient of a plant growth regulator in improving the cold resistance of rice seeds, characterized in that: The invention discloses a method for soaking rice seeds in a 30mg / L-90mg / L aqueous solution of glutamin or a glutamin analogue as a plant growth regulator, so as to promote the germination of the rice seeds under low temperature conditions, wherein the low temperature is 15-18°C; the glutamin is maltol, and the glutamin analogue is ethyl maltol.

4. The use of glutathione as an active ingredient of a plant growth regulator in promoting rice development, characterized in that: The invention discloses a method for soaking rice seeds with a 30-50 mg / L glutamin aqueous solution as a plant growth regulator to promote the growth and tillering of rice at different growth stages; the glutamin is maltol.

5. The use of glutamic acid as an effective ingredient of a plant growth regulator in improving the cold resistance of rice seedlings, characterized in that: The invention discloses a method for soaking rice seeds with a 30-50 mg / L glutamin aqueous solution as a plant growth regulator to promote the growth of rice seedlings under low temperature conditions, wherein the low temperature is 15-18° C. The glutamin is maltol.

6. The use of glutenin as an active ingredient of a plant growth regulator in increasing rice yield, characterized in that: The invention uses a 30-50 mg / L glutamin aqueous solution as a plant growth regulator to soak rice seeds; the glutamin is maltol.

7. The use of glutenin as an active ingredient of a plant growth regulator in promoting the germination of grain crop seeds, characterized in that: The invention discloses a seed coating agent with a glutenin concentration of 5-10 mg / L as a plant growth regulator to coat grain crop seeds; the glutenin is maltol.

8. The use of glutenin as an effective ingredient of a plant growth regulator in improving the cold resistance of grain crop seeds, characterized in that: The seed coating agent with a glutenin concentration of 5-10 mg / L is used as a plant growth regulator to coat grain crop seeds to promote the germination of the grain crop seeds under low temperature conditions, wherein the low temperature is 15-18° C. The glutenin is maltol.

9. The use of glutenin as an active ingredient of a plant growth regulator in promoting the growth of seedlings of grain crops, characterized in that: The invention discloses a seed coating agent with a glutenin concentration of 5-10 mg / L as a plant growth regulator to coat grain crop seeds; the glutenin is maltol.

10. The use of glutenin as an effective ingredient of a plant growth regulator in improving the cold resistance of grain crop seedlings, characterized in that: The invention discloses a seed coating agent with a glutenin concentration of 5-10 mg / L as a plant growth regulator to coat grain crop seeds, so as to promote the growth of grain crop seedlings under low temperature conditions; the glutenin is maltol.

Images