Technical method for immunizing plant diseases and insect pests

Abstract

The invention discloses a technical method for immunizing plant diseases and insect pests, which comprises the following steps: S1, information acquisition: firstly, acquiring plant leaves in a plant planting area, and classifying the acquired plant leaves into plant disease and insect pest leaves and normal leaves; s2, disease and insect pest leaf analysis: performing disease and insect pest analysis on the disease and insect pest leaves obtained in the S1 so as to obtain types, reasons and harm degrees of diseases and pests; s3, disease and pest control: specifically performing immune control on the plants by preventing the types of diseases and pests generated in plant growth so as to improve the disease and pest resistance of the plants, the vegetation immune agent prepared by the method has good action effect and good infiltration capacity, can be easily absorbed and utilized by the plants, and has a good application prospect. The fertilizer has obvious effects of preventing diseases, preventing freezing, increasing yield and improving quality, is harmless to people and livestock, and does not pollute the environment.

Description

Technical Field

[0001] The invention relates to the technical field of plant disease and insect pest control, and in particular to a technical method for immunizing plant diseases and insect pests. Background Art

[0002] Plants are different from animals. No matter what danger they encounter, they cannot escape and can only resist in place. Therefore, in order to survive and continue, plants have developed their special protective ability, which is called plant immune resistance.

[0003] When plants are stimulated by external factors or are in adverse conditions, they can produce immune responses by adjusting their own defense and metabolic systems. This defense response or immune resistance response of plants can delay or reduce the occurrence and development of diseases, reduce the use of chemical pesticides and chemical fertilizers, and reduce agricultural product residues. Plant immune inducers do not have a direct killing effect on crop pests and diseases. Instead, exogenous organisms or molecules induce or activate resistance substances produced by plants, thereby producing resistance to certain pathogens or inhibiting the growth of pathogens. When applied to crops, they induce crops to produce substances that resist or control crop pests and diseases, thereby achieving the purpose of preventing and controlling pests and diseases.

[0004] Among them, plant immune inducers have a large molecular weight of their main components and are not easy to penetrate the cuticle of plants to be efficiently absorbed and utilized. Therefore, we have proposed a technical method for immunizing plant diseases and pests to solve the above problems. Summary of the invention

[0005] Based on the technical problems existing in the background technology, the present invention proposes a technical method for immunizing plant diseases and insect pests.

[0006] The present invention provides a technical method for immunizing plant diseases and insect pests, comprising the following steps: S1: Information collection: First, plant leaves are collected in the plant planting area, and the collected plant leaves are classified into diseased and insect-infested leaves and normal leaves; S2: Pest and disease leaf analysis: Pest and disease leaf analysis is performed on the pest and disease leaves obtained in S1, so as to obtain the type, cause and degree of damage of the pest and disease; S3: Disease and pest control: By preventing the types of diseases and pests that occur during plant growth, targeted immunity control is carried out on plants, thereby improving the plant's ability to resist diseases and pests; S4: Plant immunizer: Enhance plant disease and insect pest resistance by preparing plant immunizer, and the immunizer includes chitosan, immune protein, protein protective agent and amino acid; S5: immune protein pretreatment: adding the immune protein to the buffer solution and stirring, and adding the protein protective agent during the stirring process to mix, thereby obtaining raw material 1; S6: dissolving chitosan in a buffer solution, stirring thoroughly to prepare a chitosan solution, degrading the chitosan solution, filtering, adding amino acids, and obtaining raw material 2; S7: adding the raw material 1 obtained in S5 and the raw material 2 obtained in S6 into water and mixing them to prepare a plant immune protein agent; S8: Spraying the plant immune protein agent obtained in S7 onto plant leaves or rhizomes to stimulate the plant's ability to be immune to plant diseases and insect pests.

[0007] Preferably, the stirring speed in S5 is 100-200 r / min, and the mixing and stirring is performed for 0.5-1 h.

[0008] Preferably, 0.1-0.3 mol of vitamin C is added to the chitosan solution in S6 and stirred evenly.

[0009] Preferably, the buffer solution is an acetic acid-sodium acetate solution, and the pH value of the buffer solution is between 4-6.

[0010] Preferably, the buffer in S5 is set to be a phosphate buffer, and the pH value of the phosphate buffer is 7.0-8.0.

[0011] Preferably, the mixed solution of S7 is heated in a water bath with the assistance of ultrasound for 8-10 hours.

[0012] Preferably, the ratio of raw material 1 to raw material 2 to water in S7 is 2-3:1-2:5.

[0013] Preferably, the temperature of the water bath is set to 40-50°C.

[0014] Beneficial effects of this method: (1) It can collect and analyze information on plant diseases and insect pests, stimulate immunity by preparing plant immune agents, and has significant effects in preventing diseases, preventing frost, increasing production, and improving quality. It is harmless to humans and animals and does not pollute the environment; (2) The prepared plant immunizer has good effect and good penetration ability, and is easily absorbed and utilized by plants. Implementation

[0015] The present invention will be further explained below in conjunction with specific embodiments. Example

[0016] This embodiment proposes a technical method for immunizing plant diseases and insect pests, comprising the following steps: S1: Information collection: First, plant leaves are collected in the plant planting area, and the collected plant leaves are classified into diseased and insect-infested leaves and normal leaves; S2: Pest and disease leaf analysis: Pest and disease leaf analysis is performed on the pest and disease leaves obtained in S1, so as to obtain the type, cause and degree of damage of the pest and disease; S3: Disease and pest control: By preventing the types of diseases and pests that occur during plant growth, targeted immunity control is carried out on plants, thereby improving the plant's ability to resist diseases and pests; S4: Plant immunizer: Enhance plant disease and insect pest resistance by preparing plant immunizer, and the immunizer includes chitosan, immune protein, protein protective agent and amino acid; S5: immune protein pretreatment: adding the immune protein to the buffer solution and stirring, and adding the protein protective agent during the stirring process to mix, thereby obtaining raw material 1; S6: dissolving chitosan in a buffer solution, stirring thoroughly to prepare a chitosan solution, degrading the chitosan solution, filtering, adding amino acids, and obtaining raw material 2; S7: adding the raw material 1 obtained in S5 and the raw material 2 obtained in S6 into water and mixing them to prepare a plant immune protein agent; S8: spraying the plant immune protein agent obtained in S7 onto plant leaves or rhizomes to stimulate the plant's ability to resist plant diseases and insect pests; In this embodiment, the stirring speed in S5 is 100r / min, and the mixing and stirring is performed for 0.5h. 0.1mol of vitamin C is added to the chitosan solution in S6 and stirred evenly. The buffer solution is acetic acid-sodium acetate solution, and the pH value of the buffer solution is 4. The buffer in S5 is set to a phosphate buffer, and the pH value of the phosphate buffer is 7.0.

[0017] In this embodiment, the mixed solution of S7 is heated in a water bath for 8 hours with the assistance of ultrasound, the ratio of raw material 1 to raw material 2 to water of S7 is 2:1:5, and the temperature of the water bath is set to 40°C. Example

[0018] This embodiment proposes a technical method for immunizing plant diseases and insect pests, comprising the following steps: S1: Information collection: First, plant leaves are collected in the plant planting area, and the collected plant leaves are classified into diseased and insect-infested leaves and normal leaves; S2: Pest and disease leaf analysis: Pest and disease leaf analysis is performed on the pest and disease leaves obtained in S1, so as to obtain the type, cause and degree of damage of the pest and disease; S3: Disease and pest control: By preventing the types of diseases and pests that occur during plant growth, targeted immunity control is carried out on plants, thereby improving the plant's ability to resist diseases and pests; S4: Plant immunizer: Enhance plant disease and insect pest resistance by preparing plant immunizer, and the immunizer includes chitosan, immune protein, protein protective agent and amino acid; S5: immune protein pretreatment: adding the immune protein to the buffer solution and stirring, and adding the protein protective agent during the stirring process to mix, thereby obtaining raw material 1; S6: dissolving chitosan in a buffer solution, stirring thoroughly to prepare a chitosan solution, degrading the chitosan solution, filtering, adding amino acids, and obtaining raw material 2; S7: adding the raw material 1 obtained in S5 and the raw material 2 obtained in S6 into water and mixing them to prepare a plant immune protein agent; S8: spraying the plant immune protein agent obtained in S7 onto plant leaves or rhizomes to stimulate the plant's ability to resist plant diseases and insect pests; In this embodiment, the stirring speed in S5 is 150r / min, and the mixing and stirring is performed for 0.6h. 0.2mol of vitamin C is added to the chitosan solution in S6 and stirred evenly. The buffer solution is acetic acid-sodium acetate solution, and the pH value of the buffer solution is 5. The buffer in S5 is set to a phosphate buffer, and the pH value of the phosphate buffer is 7.0.

[0019] In this embodiment, the mixed solution of S7 is heated in a water bath with the assistance of ultrasound for 9 hours, the ratio of raw material 1 to raw material 2 to water of S7 is 2:2:5, and the temperature of the water bath is set to 40-50°C. Example

[0020] This embodiment proposes a technical method for immunizing plant diseases and insect pests, comprising the following steps: S1: Information collection: First, plant leaves are collected in the plant planting area, and the collected plant leaves are classified into diseased and insect-infested leaves and normal leaves; S2: Pest and disease leaf analysis: Pest and disease leaf analysis is performed on the pest and disease leaves obtained in S1, so as to obtain the type, cause and degree of damage of the pest and disease; S3: Disease and pest control: By preventing the types of diseases and pests that occur during plant growth, targeted immunity control is carried out on plants, thereby improving the plant's ability to resist diseases and pests; S4: Plant immunizer: Enhance plant disease and insect pest resistance by preparing plant immunizer, and the immunizer includes chitosan, immune protein, protein protective agent and amino acid; S5: immune protein pretreatment: adding the immune protein to the buffer solution and stirring, and adding the protein protective agent during the stirring process to mix, thereby obtaining raw material 1; S6: dissolving chitosan in a buffer solution, stirring thoroughly to prepare a chitosan solution, degrading the chitosan solution, filtering, adding amino acids, and obtaining raw material 2; S7: adding the raw material 1 obtained in S5 and the raw material 2 obtained in S6 into water and mixing them to prepare a plant immune protein agent; S8: spraying the plant immune protein agent obtained in S7 onto plant leaves or rhizomes to stimulate the plant's ability to resist plant diseases and insect pests; In this embodiment, the stirring speed in S5 is 180r / min, and the mixing and stirring is performed for 0.8h. 0.2mol of vitamin C is added to the chitosan solution in S6 and stirred evenly. The buffer solution is acetic acid-sodium acetate solution, and the pH value of the buffer solution is 6. The buffer in S5 is set to a phosphate buffer, and the pH value of the phosphate buffer is 8.0.

[0021] In this embodiment, the mixed solution of S7 is heated in a water bath for 10 hours with the assistance of ultrasound, the ratio of raw material 1 to raw material 2 to water of S7 is 3:1:5, and the temperature of the water bath is set to 45°C. Example

[0022] This embodiment proposes a technical method for immunizing plant diseases and insect pests, comprising the following steps: S1: Information collection: First, plant leaves are collected in the plant planting area, and the collected plant leaves are classified into diseased and insect-infested leaves and normal leaves; S2: Pest and disease leaf analysis: Pest and disease leaf analysis is performed on the pest and disease leaves obtained in S1, so as to obtain the type, cause and degree of damage of the pest and disease; S3: Disease and pest control: By preventing the types of diseases and pests that occur during plant growth, targeted immunity control is carried out on plants, thereby improving the plant's ability to resist diseases and pests; S4: Plant immunizer: Enhance plant disease and insect pest resistance by preparing plant immunizer, and the immunizer includes chitosan, immune protein, protein protective agent and amino acid; S5: immune protein pretreatment: adding the immune protein to the buffer solution and stirring, and adding the protein protective agent during the stirring process to mix, thereby obtaining raw material 1; S6: dissolving chitosan in a buffer solution, stirring thoroughly to prepare a chitosan solution, degrading the chitosan solution, filtering, adding amino acids, and obtaining raw material 2; S7: adding the raw material 1 obtained in S5 and the raw material 2 obtained in S6 into water and mixing them to prepare a plant immune protein agent; S8: spraying the plant immune protein agent obtained in S7 onto plant leaves or rhizomes to stimulate the plant's ability to resist plant diseases and insect pests; In this embodiment, the stirring speed in S5 is 200r / min, and the mixing and stirring is performed for 1h, 0.3mol of vitamin C is added to the chitosan solution in S6 and stirred evenly, the buffer solution is acetic acid-sodium acetate solution, the pH value of the buffer solution is 6, the buffer in S5 is set to phosphate buffer, and the pH value of the phosphate buffer is 8.0.

[0023] In this embodiment, the mixed solution of S7 is heated in a water bath for 10 hours with the assistance of ultrasound, the ratio of raw material 1 to raw material 2 to water of S7 is 3:2:5, and the temperature of the water bath is set to 50°C.

[0024] This method can collect and analyze information on plant diseases and insects, stimulate immunity by preparing vegetation immune agents, and has significant effects of preventing diseases, preventing frost, increasing production, and improving quality. It is harmless to humans and animals and does not pollute the environment. The prepared vegetation immune agents have good effects and good penetration ability, and are easily absorbed and utilized by plants.

[0025] The vegetation immunizing agents prepared in Examples 1 to 4 were selected and sprayed on 100 plants, as shown in the table; Example Root penetration rate (%) Leaf penetration rate (%) Pest and disease immunity (%) Embodiment 1 95.9 98.8 98.9 Embodiment 2 94.7 99.1 99.6 Embodiment 3 97.6 99.3 99.8 Embodiment 4 98.9 99.8 99.9

[0026] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A technical method for immunizing plant diseases and insect pests, characterized in that: The following steps are involved: S1: Information collection: First, plant leaves are collected in the plant planting area, and the collected plant leaves are classified into diseased and insect-infested leaves and normal leaves; S2: Pest and disease leaf analysis: Pest and disease leaf analysis is performed on the pest and disease leaves obtained in S1, so as to obtain the type, cause and degree of damage of the pest and disease; S3: Disease and pest control: By preventing the types of diseases and pests that occur during plant growth, targeted immunity control is carried out on plants, thereby improving the plant's ability to resist diseases and pests; S4: Plant immunizer: Enhance plant disease and insect pest resistance by preparing plant immunizer, and the immunizer includes chitosan, immune protein, protein protective agent and amino acid; S5: immune protein pretreatment: adding the immune protein to the buffer solution and stirring, and adding the protein protective agent during the stirring process to mix, thereby obtaining raw material 1; S6: dissolving chitosan in a buffer solution, stirring thoroughly to prepare a chitosan solution, degrading the chitosan solution, filtering, adding amino acids, and obtaining raw material 2; S7: adding the raw material 1 obtained in S5 and the raw material 2 obtained in S6 into water and mixing them to prepare a plant immune protein agent; S8: Spraying the plant immune protein agent obtained in S7 onto plant leaves or rhizomes to stimulate the plant's ability to be immune to plant diseases and insect pests.

2. The technical method for immunizing plant diseases and insect pests according to claim 1, characterized in that: The stirring speed in S5 is 100-200 r / min, and the mixing and stirring is performed for 0.5-1 h.

3. The technical method for immunizing plant diseases and insect pests according to claim 1, characterized in that: Add 0.1-0.3 mol of vitamin C to the chitosan solution in S6 and stir evenly.

4. The technical method for immunizing plant diseases and insect pests according to claim 1, characterized in that: The buffer solution is an acetic acid-sodium acetate solution, and the pH value of the buffer solution is between 4 and 6.

5. The technical method for immunizing plant diseases and insect pests according to claim 1, characterized in that: The buffer in S5 is set to be a phosphate buffer, and the pH value of the phosphate buffer is 7.0-8.

0.

6. The technical method for immunizing plant diseases and insect pests according to claim 1, characterized in that: The S7 mixture is heated in a water bath with the aid of ultrasonic waves for 8-10 hours.

7. The technical method for immunizing plant diseases and insect pests according to claim 1, characterized in that: The ratio of raw material 1 to raw material 2 to water in S7 is 2-3:1-2:

5.

8. The technical method for immunizing plant diseases and insect pests according to claim 6, characterized in that: The temperature of the water bath was set to 40-50°C.