Tobacco suckercide and preparation method thereof

Abstract

The invention relates to the technical field of tobacco cultivation, and discloses a tobacco bud inhibitor and a preparation method thereof. The tobacco bud inhibitor comprises the following components in percentage by mass: 5%-20% of indole-3-methyl acetate, 35%-70% of a ketone solvent, 8%-20% of an emulsifier and the balance of deionized water. The preparation method of the tobacco suckercide comprises the following steps: dissolving indole-3-methyl acetate in a ketone solvent to form a solution; adding an emulsifier into the solution, and uniformly stirring to form an oil phase; and adding deionized water into the oil phase, and stirring according to preset stirring parameters to obtain the tobacco suckercide. In the application, through the synergistic effect of the indole-3-methyl acetate, the ketone solvent, the emulsifier and the deionized water, not only can effective bud inhibition be realized, but also drug residues can be reduced, and the quality of tobacco leaves can be improved.

Description

Technical Field

[0001] The present application belongs to the technical field of tobacco cultivation, and specifically relates to a tobacco sprout inhibitor and a preparation method thereof. Background Art

[0002] Types of tobacco sprout inhibitors include chemical sprout inhibitors, such as brassinolide, calcium chloride, ethephon, etc.

[0003] In actual application, although chemical sprout inhibitors can inhibit the growth of tobacco buds, there is a problem of drug residues, which affects the quality of tobacco leaves. Moreover, long-term use of chemical sprout inhibitors may also cause environmental pollution. Summary of the invention

[0004] The present application aims to solve at least one of the technical problems existing in the related art.

[0005] Therefore, the first aspect of the present application provides a tobacco sprout inhibitor.

[0006] The second aspect of the present application provides a method for preparing a tobacco sprout inhibitor.

[0007] In view of this, according to the first aspect of an embodiment of the present application, a tobacco bud inhibitor is proposed, which includes the following components by mass percentage: 5% to 20% indole-3-acetic acid methyl ester, 35% to 70% ketone solvent, 8% to 20% emulsifier, and the rest is deionized water.

[0008] Optionally, the ketone solvent includes one or more of acetone, isophorone and cyclohexanone.

[0009] Optionally, the emulsifier includes one or more of Nong Ru No. 400, Nong Ru No. 500, Nong Ru No. 600 and Nong Ru No. 1602.

[0010] Optionally, the tobacco sprout inhibitor further comprises the following components by mass percentage: 1% to 5% of a co-surfactant, and / or 1% to 5% of an antifreeze agent.

[0011] Optionally, the co-surfactant includes one or more of ethanol, n-propanol, isopropanol, n-butanol, isobutanol, n-pentanol, isopentanol, 1-hexanol, 2-hexanol, 1-octanol and 2-octanol.

[0012] Optionally, the antifreeze agent includes one or more of ethylene glycol, propylene glycol, glycerol and polyethylene glycol.

[0013] According to the second aspect of the embodiments of the present application, a method for preparing a tobacco bud inhibitor is proposed, which is used to prepare the tobacco bud inhibitor as in any of the previous embodiments. The preparation method comprises the following steps: dissolving the indole-3-acetic acid methyl ester in the ketone solvent to form a solution; adding the emulsifier to the solution and stirring evenly to form an oil phase; adding the deionized water to the oil phase and stirring according to preset stirring parameters to obtain the tobacco bud inhibitor.

[0014] Optionally, the preset stirring parameters include: stirring speed: 10000 r / min to 12000 r / min; and / or, stirring time: 1 min to 3 min.

[0015] Optionally, in the step of adding the emulsifier to the solution, a co-surfactant is also added to the solution.

[0016] Optionally, in the step of adding the deionized water to the oil phase, an antifreeze agent is also added to the oil phase.

[0017] The tobacco bud inhibitor and preparation method thereof provided in this application can at least achieve the following technical effects:

[0018] In the present application, the growth of buds can be effectively inhibited by indole-3-acetic acid methyl ester. Indole-3-acetic acid methyl ester has biological activity and can be metabolized by plants to reduce residues. Ketone solvents can achieve volatilization and degradation, which helps to reduce pesticide residues. Emulsifiers and deionized water help tobacco bud inhibitors to be evenly distributed on the surface of tobacco leaves, reducing residues caused by local excess. In the present application, through the synergistic effect of indole-3-acetic acid methyl ester, ketone solvents, emulsifiers and deionized water, effective bud inhibition can be achieved, drug residues can be reduced, and the quality of tobacco leaves can be improved.

[0019] The above general description and the following description are exemplary and explanatory only and are not intended to limit the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] One or more embodiments are exemplarily described by corresponding drawings, which do not limit the embodiments. Elements with the same reference numerals in the drawings are shown as similar elements, and the drawings do not constitute a scale limitation, and wherein:

[0021] Figure 1 A flow chart of a method for preparing a tobacco sprout inhibitor provided by an embodiment of the present disclosure;

[0022] Figure 2 A flow chart of a method for preparing a tobacco sprout inhibitor provided by another embodiment of the present disclosure;

[0023] Figure 3A high performance liquid chromatogram of a tobacco bud inhibitor sample before a hot storage experiment provided in Example 1 of the present disclosure;

[0024] Figure 4 This is a high performance liquid chromatogram of the tobacco bud inhibitor sample after the hot storage experiment provided in Example 1 of the present disclosure. DETAILED DESCRIPTION

[0025] In order to be able to understand the features and technical contents of the embodiments of the present disclosure in more detail, the implementation of the embodiments of the present disclosure is described in detail below in conjunction with the accompanying drawings, and the attached drawings are only for reference and explanation purposes and are not used to limit the embodiments of the present disclosure. In the following technical description, for the convenience of explanation, a plurality of details are provided to provide a full understanding of the disclosed embodiments. However, one or more embodiments can still be implemented without these details.

[0026] Unless otherwise stated, the term "plurality" means two or more.

[0027] In the embodiment of the present disclosure, the character " / " indicates that the preceding and following objects are in an "or" relationship. For example, A / B indicates: A or B.

[0028] The term "and / or" is a description of the association relationship between objects, indicating that three relationships can exist. For example, A and / or B means: A or B, or, A and B.

[0029] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present disclosure may be combined with each other.

[0030] It should be noted that the term "water emulsion (EW)" used in the embodiments of the present disclosure, also known as concentrated emulsion, is a water-insoluble solution obtained by dissolving a water-insoluble liquid technical or solid technical in an organic solvent, dispersed in water, and formed into an environmentally friendly pesticide formulation. By adding adjuvants and through processing technology, the oil phase is evenly distributed in the water phase to form a stable water emulsion system. The oil droplet size of the water emulsion is usually 0.7μm to 20μm, and ideally 1.5μm to 3.5μm.

[0031] It should be noted that the cold storage test used in the examples of the present disclosure is used to evaluate the cold storage stability. The specific method is: take 5 ml of the sample into a 10 ml stoppered test tube, place it in a refrigerator at a temperature of (0±1)°C, and store it for 7 days. If the sample can maintain a uniform state, without floating oil on the top and no precipitation on the bottom, the cold storage stability is qualified.

[0032] It should be noted that the hot storage test used in the examples of the present disclosure is used to evaluate the hot storage stability. The specific method is: take 5 ml of the sample into a 10 ml stoppered test tube, place it in a thermostat at a temperature of (54±2)°C and store it for 14 days. If the appearance of the sample remains uniform and transparent, the hot storage stability is qualified. If a small amount of stratification occurs, it can be restored to its original state after slight shaking, which is also considered to be qualified for hot storage stability.

[0033] It should be noted that the room temperature storage experiment used in the examples of the present disclosure is used to evaluate room temperature stability. The specific method is: take 5 ml of sample in a 10 ml stoppered test tube and store it at room temperature away from light. If the sample appearance is uniform, the room temperature stability is qualified.

[0034] It should be noted that the emulsion stability test used in the embodiments of the present disclosure is used to evaluate the stability of the emulsion. Emulsion stability refers to the stability of the emulsion formed after the water emulsion is diluted with water over time. According to the emulsion and emulsion stability test method, that is, the method in GB / T1603-2001 is used for determination. In a 250mL beaker, 100mL of standard hard water with a temperature of 25°C to 30°C is added, and 0.5mL of water emulsion sample (diluted 200 times) is drawn with a pipette. Slowly add to the standard hard water while stirring continuously. After adding the water emulsion, continue to stir at a speed of 2r / s to 3r / s for 30s, immediately move the emulsion to a clean, dry 100mL measuring cylinder, and place the measuring cylinder in a constant temperature water bath, within the range of (30±2)°C, let it stand for 1h, and observe the separation of the emulsion. If there is no floating oil, precipitation, and sunken oil precipitation in the measuring cylinder, the emulsion stability is qualified.

[0035] It should be noted that the evaluation standard for the emulsion dispersibility used in the embodiments of the present disclosure is: according to the requirements of the emulsion stability determination method in the national standard GB / T1603-2001, under the condition of 25±1°C, 1 mL of the preparation sample is sucked at a distance of 2 cm from the water surface, and slowly dripped into a beaker containing 200 mL of standard hard water, diluted and observed. The dispersion state, emulsification state and evaluation level of the oil phase (preparation sample) in water are shown in Table 1. That is, as shown in Table 1, when the emulsion dispersibility level of the preparation sample is level 1, it is marked as "+++", the dispersion state is that it can be quickly and automatically dispersed evenly, and the emulsification state is a blue or milky white transparent emulsion with a little stirring. When the emulsion dispersibility level of the preparation sample is level 2, it is marked as "++", the dispersion state is that it can be automatically and evenly dispersed, and the emulsification state is a blue translucent emulsion with a little stirring. When the emulsion dispersibility level of the preparation sample is level 3, it is marked as "+", the dispersion state is white cloud-like or filamentous dispersion, and the emulsified state is a blue opaque emulsion after stirring. When the emulsion dispersibility level of the preparation sample is level 4, it is marked as "-", the dispersion state is white particles sinking, and the emulsified state is a white-blue opaque emulsion after stirring. When the emulsion dispersibility level of the preparation sample is level 5, it is marked as "--", the dispersion state is oil droplets sinking, the emulsified state can be emulsified after stirring, and stratification is quickly achieved after stopping stirring.

[0036] Table 1 Dispersion state, emulsification state and evaluation level of oil phase in water

[0037]

[0038] The embodiment of the present disclosure provides a tobacco bud inhibitor, which includes the following components by mass percentage: 5% to 20% indole-3-acetic acid methyl ester, 35% to 70% ketone solvent, 8% to 20% emulsifier, and the rest is deionized water.

[0039] Specifically, in the tobacco bud suppressor, the mass of indole-3-acetic acid methyl ester accounts for 5% to 20% of the total mass of the tobacco bud suppressor. The mass of ketone solvents accounts for 35% to 70% of the total mass of the tobacco bud suppressor. The mass of the emulsifier accounts for 8% to 20% of the total mass of the tobacco bud suppressor, and the rest is deionized water. That is to say, in the tobacco bud suppressor, the content of indole-3-acetic acid methyl ester is 5% to 20%, the content of ketone solvents is 35% to 70%, the content of emulsifier is 8% to 20%, and the rest is deionized water.

[0040] In this embodiment, the tobacco bud suppressor comprises indole-3-acetic acid methyl ester, ketone solvent, emulsifier and deionized water. After indole-3-acetic acid methyl ester is dissolved in the ketone solvent, the emulsifier is added to form an oil phase. Deionized water is used to provide an aqueous phase. Through the oil phase and the aqueous phase, the dosage form of the tobacco bud suppressor can be an aqueous emulsion, thereby achieving safety, environmental protection, and economic efficiency of the tobacco bud suppressor.

[0041] In the present embodiment, the growth of buds can be effectively inhibited by indole-3-acetic acid methyl ester. Indole-3-acetic acid methyl ester belongs to auxin compounds, has biological activity, can be metabolized by plants, and reduces residues. Ketone solvents can achieve volatilization and degradation, which helps to reduce pesticide residues. Emulsifiers and deionized water help tobacco bud inhibitors to be evenly distributed on the surface of tobacco leaves, reducing the residues caused by local excess. Through the synergistic effect of indole-3-acetic acid methyl ester, ketone solvents, emulsifiers and deionized water, effective bud inhibition can be achieved, drug residues can also be reduced, and the quality of tobacco leaves can be improved.

[0042] When applied to tobacco plants, the aqueous emulsion type tobacco bud inhibitor can be evenly distributed on the surface of the tobacco plant, achieving effective coverage of the agent and improving the bud inhibition effect. The combination of the aqueous phase and the oil phase in the tobacco bud inhibitor gives it good adhesion and permeability, which can act more deeply on the bud points and improve the durability and stability of bud inhibition. The aqueous phase in the tobacco bud inhibitor can also reduce the use of organic solvents and reduce costs. The tobacco bud inhibitor also has good biocompatibility. Through the biological activity of indole-3-acetic acid methyl ester, residues can be reduced, which helps to achieve green production of tobacco.

[0043] It can be understood that the tobacco sprout inhibitor of this embodiment can also be used for other plants to achieve effective sprout inhibition while reducing drug residues.

[0044] Optionally, in the tobacco bud inhibitor, the content of indole-3-acetic acid methyl ester is 5%, 10%, 15%, 20% or other values ​​between 5% and 20%.

[0045] Optionally, in the tobacco bud inhibitor, the content of the ketone solvent is 35%, 40%, 50%, 60%, 70% or other values ​​between 35% and 70%.

[0046] Optionally, in the tobacco bud inhibitor, the content of the emulsifier is 8%, 12%, 15%, 20% or other values ​​between 8% and 20%.

[0047] Exemplarily, indole-3-acetic acid methyl ester is the product of indole-3-acetic acid, methyl esterified by the indoleacetic acid carboxyl methyltransferase 1 (IAMT1) gene. Indole-3-acetic acid methyl ester can freely enter and exit cells, and is another form of plant regulation of indole-3-acetic acid activity in plants. In the early stage, our research group obtained the ascorbic acid Kluyveraascorbata ZLSY22 strain from the digestive tract of the parasitic insect Procecidochares utilis Stone of purple stem Eupatorium in Yunnan. The fermentation broth of this strain can inhibit the germination of seeds such as purple stem Eupatorium. A total of 41 compounds were isolated from the precipitate and supernatant of the ZLSY22 fermentation broth. Four compounds with herbicidal activity were screened out using seed germination method, seedling cup method and other methods. Among them, indole-3-acetic acid methyl ester has a good inhibitory effect on purple stem Eupatorium and its seedlings.

[0048] In some embodiments, the ketone solvent includes one or more of acetone, isophorone, and cyclohexanone.

[0049] In the present embodiment, ketone solvents include one or more of acetone, isophorone and cyclohexanone, and have good solubility to the original drug (indole-3-acetic acid methyl ester). Ketone solvents can achieve volatilization and degradation, which helps to reduce pesticide residues. The physicochemical properties of ketone solvents are stable, and ketone solvents can reduce surface tension and increase fluidity together with emulsifiers.

[0050] For example, taking solid indole-3-acetic acid methyl ester as an example, the dissolution of indole-3-acetic acid methyl ester (solute) in acetone, isophorone and cyclohexanone was observed experimentally, and the results are shown in Table 2.

[0051] Table 2 Dissolution of indole-3-acetic acid methyl ester in ketone solvents

[0052] Solvent Solute / Solvent (g / mL) Dissolved state Cold storage stability Quality qualification acetone 1 / 2 Dissolve qualified qualified Isophorone 1 / 3 Dissolve qualified qualified Cyclohexanone 1 / 2 Dissolve qualified qualified

[0053] As can be seen from Table 2, when observing the dissolution at room temperature, indole-3-acetic acid methyl ester can be dissolved in acetone, and the solute dosage is small, which has a good dissolution effect. In addition, the original drug (indole-3-acetic acid methyl ester) does not precipitate at (0±1)℃, and can maintain a uniform state, without floating oil on the top and no precipitation on the bottom, and the cold storage stability is qualified. Therefore, the quality qualification is qualified.

[0054] As can be seen from Table 2, when observing the dissolution at room temperature, indole-3-acetic acid methyl ester can be dissolved in isophorone, and the solute dosage is small, which has a good dissolution effect. In addition, the original drug (indole-3-acetic acid methyl ester) does not precipitate at (0±1)℃, and can maintain a uniform state, without floating oil on the top and no precipitation on the bottom, and the cold storage stability is qualified. Therefore, the quality qualification is qualified.

[0055] As can be seen from Table 2, when observing the dissolution at room temperature, indole-3-acetic acid methyl ester can be dissolved in cyclohexanone, and the solute dosage is small, which has a good dissolution effect. In addition, the original drug (indole-3-acetic acid methyl ester) does not precipitate at (0±1)℃, and can maintain a uniform state, without floating oil on the top and no precipitation on the bottom, and the cold storage stability is qualified. Therefore, the quality qualification is qualified.

[0056] In some embodiments, the emulsifier includes one or more of Nong Ru No. 400, Nong Ru No. 500, Nong Ru No. 600 and Nong Ru No. 1602.

[0057] In this embodiment, the emulsifier includes one or more of Nongru No. 400, Nongru No. 500, Nongru No. 600 and Nongru No. 1602. The emulsifier is combined with the ketone solvent to reduce surface tension and increase fluidity. The emulsifier is combined with deionized water to help the tobacco bud inhibitor to be evenly distributed on the surface of the tobacco leaves and reduce the residue caused by local excess.

[0058] In this embodiment, Nong Ru No. 400, Nong Ru No. 500, Nong Ru No. 600 and Nong Ru No. 1602 have good emulsification effects, which are helpful to enhance the sprout inhibitory effect of the tobacco sprout inhibitor and reduce the effect of drug residue.

[0059] For example, Nongru No. 400, Nongru No. 500, Nongru No. 600 and Nongru No. 1602 were subjected to room temperature storage experiment, hot storage experiment and emulsion dispersibility evaluation to evaluate the emulsification effect. The results are shown in Table 3.

[0060] Table 3 Emulsifying effect of emulsifier

[0061]

[0062] In this example, it can be seen from Table 3 that the room temperature stability of Nongru No. 400 is qualified, the heat storage stability is qualified, and the level of emulsion dispersibility is level 1, so the quality qualification is qualified. The room temperature stability of Nongru No. 500 is qualified, the heat storage stability is qualified, and the level of emulsion dispersibility is level 2, so the quality qualification is qualified. The room temperature stability of Nongru No. 600 is qualified, the heat storage stability is qualified, and the level of emulsion dispersibility is level 1, so the quality qualification is qualified. The room temperature stability of Nongru No. 1602 is qualified, the heat storage stability is qualified, and the level of emulsion dispersibility is level 2, so the quality qualification is qualified. In other words, the emulsification effects of Nongru No. 400, Nongru No. 500, Nongru No. 600 and Nongru No. 1602 are all good, which helps to improve the bud inhibition effect of tobacco bud inhibitors and reduce the effect of drug residues.

[0063] As a comparative example, the emulsification effects of emulsifiers NP-10, OP-10, Tween 80, Tween 20, Span 80 and Span 20 were evaluated through room temperature storage experiments, hot storage experiments and emulsion dispersibility evaluation. The results are shown in Table 3.

[0064] In this comparative example, it can be seen from Table 3 that the room temperature stability of emulsifier NP-10 is unqualified (stratification), the heat storage stability is unqualified (stratification), and the level of emulsion dispersibility is level 5, so the quality qualification is unqualified. The room temperature stability of emulsifier OP-10 is unqualified (stratification), the heat storage stability is unqualified (stratification), and the level of emulsion dispersibility is level 5, so the quality qualification is unqualified. Tween 80 is unqualified in room temperature stability (stratification), unqualified in heat storage stability (stratification), and the level of emulsion dispersibility is level 3, so the quality qualification is unqualified. Tween 20 is qualified in room temperature stability, but unqualified in heat storage stability (stratification), and the level of emulsion dispersibility is level 5, so the quality qualification is unqualified. Span 80 is qualified in room temperature stability, but unqualified in heat storage stability (oil separation), and the level of emulsion dispersibility is level 3, so the quality qualification is unqualified. The room temperature stability of Span 20 is unqualified (stratification), the hot storage stability is unqualified (crystallization), and the emulsion dispersion level is level 5, so the quality qualification is unqualified. That is to say, if the emulsifiers NP-10, OP-10, Tween 80, Tween 20, Span 80 and Span 20 in the comparative example are used in tobacco bud inhibitors, they cannot achieve the bud inhibition effect and the effect of reducing drug residues in this application.

[0065] It can be understood that when conducting room temperature preservation experiments, hot storage experiments and emulsion dispersibility evaluations, each emulsifier can be tested multiple times, for example, three times, to achieve an accurate evaluation of the emulsification effect.

[0066] Optionally, the emulsifier includes Agricultural Ru No. 400.

[0067] Optionally, the emulsifier includes Nongru 500.

[0068] Optionally, the emulsifier includes Nongru 600.

[0069] Optionally, the emulsifier includes Nongru No. 1602.

[0070] Optionally, the emulsifier includes Nongru No. 500 and Nongru No. 600. The mass ratio of Nongru No. 600 to Nongru No. 500 is 1:2.

[0071] Optionally, the emulsifier includes Nongru No. 500 and Nongru No. 1602. The mass ratio of Nongru No. 1602 to Nongru No. 500 is 1:1.

[0072] Optionally, the emulsifier includes Nongru No. 400 and Nongru No. 600. The mass ratio of Nongru No. 600 to Nongru No. 400 is 1:1. Alternatively, the mass ratio of Nongru No. 600 to Nongru No. 400 is 2:1. Alternatively, the mass ratio of Nongru No. 600 to Nongru No. 400 is 1:2.

[0073] In some embodiments, the tobacco sprout inhibitor further comprises the following components by mass percentage: 1% to 5% of a co-surfactant, and / or 1% to 5% of an antifreeze agent.

[0074] In this embodiment, the co-surfactant is used to change the hydrophilicity and lipophilicity of the emulsifier to make the tobacco bud inhibitor more stable. The antifreeze agent is used to prevent or reduce low-temperature freezing.

[0075] Optionally, the tobacco bud inhibitor includes the following components by mass percentage: 5% to 20% indole-3-acetic acid methyl ester, 35% to 70% ketone solvent, 8% to 20% emulsifier, 1% to 5% co-surfactant, and the rest is deionized water.

[0076] Optionally, the tobacco bud inhibitor includes the following components by mass percentage: 5% to 20% indole-3-acetic acid methyl ester, 35% to 70% ketone solvent, 8% to 20% emulsifier, 1% to 5% antifreeze agent, and the rest is deionized water.

[0077] Optionally, the tobacco bud inhibitor includes the following components by mass percentage: 5% to 20% indole-3-acetic acid methyl ester, 35% to 70% ketone solvent, 8% to 20% emulsifier, 1% to 5% co-surfactant, 1% to 5% antifreeze agent, and the rest is deionized water.

[0078] Optionally, in the tobacco bud inhibitor, the content of the co-surfactant is 1%, 2%, 3%, 4%, 5% or other values ​​between 1% and 5%.

[0079] Optionally, in the tobacco bud inhibitor, the content of the antifreeze agent is 1%, 2%, 3%, 4%, 5% or other values ​​between 1% and 5%.

[0080] In some embodiments, the co-surfactant includes one or more of ethanol, n-propanol, isopropanol, n-butanol, isobutanol, n-pentanol, isopentanol, 1-hexanol, 2-hexanol, 1-octanol, and 2-octanol.

[0081] In this embodiment, the co-surfactant includes one or more of ethanol, n-propanol, isopropanol, n-butanol, isobutanol, n-pentanol, isopentanol, 1-hexanol, 2-hexanol, 1-octanol and 2-octanol, which helps to improve the sprout inhibitory effect of the tobacco sprout inhibitor, reduce drug residues and reduce costs.

[0082] In some embodiments, the antifreeze agent includes one or more of ethylene glycol, propylene glycol, glycerol, and polyethylene glycol.

[0083] In this embodiment, the antifreeze agent includes one or more of ethylene glycol, propylene glycol, glycerol and polyethylene glycol, which helps to achieve the low-temperature stability and effectiveness of the tobacco bud inhibitor.

[0084] like Figure 1 As shown, the present disclosure also provides a method for preparing a tobacco sprout inhibitor, which is used to prepare the tobacco sprout inhibitor of any of the previous embodiments. The preparation method comprises the following steps:

[0085] S101. Dissolve indole-3-acetic acid methyl ester in a ketone solvent to form a solution.

[0086] In this embodiment, indole-3-acetic acid methyl ester is dissolved in a ketone solvent to form a solution, so that the indole-3-acetic acid methyl ester is evenly dispersed in the ketone solvent, in preparation for the subsequent addition of an emulsifier.

[0087] Exemplarily, indole-3-acetic acid methyl ester (original drug) is solid. Indole-3-acetic acid methyl ester is dissolved in a ketone solvent to form a solution, so that the indole-3-acetic acid methyl ester is evenly dispersed in the ketone solvent.

[0088] S102, adding an emulsifier to the solution, stirring evenly to form an oil phase.

[0089] In this embodiment, an emulsifier is added to the solution and stirred evenly to form an oil phase for uniform mixing with the water phase. The emulsifier can be used to reduce the surface tension between oil and water and improve the stability and effectiveness of the tobacco bud inhibitor.

[0090] S103, adding deionized water to the oil phase, stirring according to preset stirring parameters, to obtain a tobacco sprout inhibitor.

[0091] In this embodiment, deionized water is used to provide the water phase. The oil phase and the water phase are stirred according to the preset stirring parameters to fully mix and form an emulsion with good stability, that is, a water-emulsion type tobacco bud inhibitor, which improves the uniformity and effectiveness of the tobacco bud inhibitor.

[0092] In this embodiment, the preparation method has a simple process and low cost. The prepared tobacco sprout inhibitor has a long storage period and stability, can effectively inhibit sprouting, reduce drug residues, improve the quality of tobacco leaves, and is suitable for large-scale production and application.

[0093] In this embodiment, the step of stirring according to preset stirring parameters can be achieved by a high-speed shearing machine.

[0094] In some embodiments, the preset stirring parameters include: stirring speed: 10000 r / min to 12000 r / min; and / or, stirring time: 1 min to 3 min.

[0095] In this embodiment, the stirring speed is 10000 r / min to 12000 r / min to achieve full mixing of the water phase and the oil phase to form a water-emulsion type tobacco bud inhibitor.

[0096] In this embodiment, the stirring time is 1 min to 3 min to achieve full mixing of the water phase and the oil phase to form a water-emulsion type tobacco bud inhibitor.

[0097] Optionally, the stirring speed is 10000 r / min, 11000 r / min, 12000 r / min or other values ​​between 10000 r / min and 12000 r / min.

[0098] Optionally, the stirring time is 1 min, 2 min, 3 min or other values ​​between 1 min and 3 min.

[0099] In some embodiments, in the step of adding the emulsifier to the solution, a co-surfactant is also added to the solution; and / or, in the step of adding deionized water to the oil phase, an antifreeze agent is also added to the oil phase.

[0100] In the present embodiment, in the step of adding the emulsifier to the solution, a cosurfactant is also added to the solution to improve the bud-inhibiting effect of the tobacco bud-inhibiting agent and reduce pesticide residues. It is understood that the specific manner of adding a cosurfactant to the solution in the step of adding the emulsifier to the solution is not limited. For example, a cosurfactant can be added to the solution together with the emulsifier, and then stirred. For another example, the emulsifier is first added to the solution, and then stirred, and a cosurfactant is added during the stirring process.

[0101] In the present embodiment, in the step of adding deionized water to the oil phase, antifreeze is also added to the oil phase to realize the low temperature stability and effectiveness of the tobacco bud suppressor. It is to be understood that the specific mode of adding antifreeze to the oil phase in the step of adding deionized water to the oil phase is not limited. For example, deionized water can be added to the solution together with antifreeze, and then stirred. For another example, deionized water is first added to the solution, and then stirred, and antifreeze is added in the process of stirring. For another example, stir the oil phase, and in the process of stirring the oil phase, add deionized water and antifreeze.

[0102] Alternatively, if Figure 2 As shown, the present disclosure also provides a method for preparing a tobacco sprout inhibitor, which is used to prepare the tobacco sprout inhibitor of any of the previous embodiments. The preparation method comprises the following steps:

[0103] S201. Dissolve indole-3-acetic acid methyl ester in a ketone solvent to form a solution.

[0104] S202, adding an emulsifier and a co-surfactant to the solution, stirring evenly to form an oil phase.

[0105] S203, adding deionized water and antifreeze agent into the oil phase, stirring according to preset stirring parameters, to obtain tobacco bud inhibitor.

[0106] Specific examples are given below to further illustrate the embodiments of the present disclosure.

[0107] Example 1

[0108] The tobacco bud suppressant in this embodiment includes the following components by mass percentage: 10% indole-3-acetic acid methyl ester, 50% acetone solvent, 15% emulsifier, 4% n-butanol, 4% ethylene glycol, and 17% deionized water. Among them, the emulsifier includes Nongru No. 500 and Nongru No. 1602, and the mass ratio of Nongru No. 1602 to Nongru No. 500 is 1:1.

[0109] The preparation method of the tobacco bud inhibitor in this embodiment comprises the following steps: dissolving indole-3-acetic acid methyl ester in acetone to form a solution. Adding an emulsifier and n-butanol to the solution, stirring evenly to form an oil phase. Under the action of a high-speed shearing machine, adding deionized water and ethylene glycol to the oil phase, wherein the stirring speed is 10000r / min and the stirring time is 3min, to obtain the tobacco bud inhibitor.

[0110] The tobacco sprout inhibitor of this embodiment can effectively suppress sprouting. The synergistic effect of indole-3-acetic acid methyl ester, acetone solvent, emulsifier, n-butanol, ethylene glycol and deionized water can reduce drug residues and improve the quality of tobacco leaves.

[0111] The tobacco bud inhibitor of this embodiment is qualified in appearance stability, emulsion stability, heat storage stability, low temperature stability, pH value, and emulsion dispersibility. Specifically, whether it is a 2-year room temperature storage experiment or an accelerated storage experiment, the appearance of the tobacco bud inhibitor is a clear and transparent liquid without stratification, floating oil, and precipitation (or crystallization), that is, the appearance stability is qualified. Emulsion stability test, no floating oil, precipitation, and sunken oil precipitation, that is, the emulsion stability is qualified. Hot storage experiment, no stratification, no oil precipitation, maintain a good emulsion state, only precipitate emulsion and water, gently shake can still form a uniform emulsion, that is, hot storage stability is qualified. Put an appropriate amount of tobacco bud inhibitor into an ampoule bottle, seal it in a refrigerator at 0℃, -5℃ or -9℃, store it for 1 week or 2 weeks, observe it, no stratification, no crystallization, that is, low temperature stability is qualified. The qualified range of the pH value of pesticides is 4 to 7. The pH value of tobacco bud inhibitor is 6.73, that is, the pH value is qualified. The level of emulsion dispersibility is 2, and the emulsion dispersibility is qualified.

[0112] According to the standards set by the Food and Agriculture Organization of the United Nations (FAO), the storage stability test of tobacco bud inhibitors is carried out, and the storage decomposition rate of indole-3-acetic acid methyl ester in the tobacco bud inhibitors must be ≤5%. Specifically, the tobacco bud inhibitor samples are chemically analyzed by high performance liquid chromatography (HPLC). The HPLC determination conditions are as follows: mobile phase: water: methanol (V / V) = 34:66, degassing filtration; flow rate: 3.0 mL / min; column temperature: 25°C; ultraviolet detection wavelength: 230 nm; injection volume: 10 μL; chromatographic column model: C185 μL, 10 nm x150 mm130 A; retention time: 5.2 min.

[0113] The tobacco bud inhibitor samples before the hot storage experiment were analyzed by HPLC, and the HPLC chromatogram was obtained as follows: Figure 3 As shown, Figure 3 In the graph, the horizontal axis represents time (unit: min), and the vertical axis represents the response value of the electrical signal (unit: mAu). Figure 3 A in the figure is used to indicate the peak area (unit: mAu·min), and the peak area of ​​indole-3-acetic acid methyl ester of the tobacco sprout inhibitor sample before the hot storage experiment is 67083.45590.

[0114] The tobacco bud inhibitor samples after the hot storage experiment were analyzed by HPLC, and the HPLC chromatogram was obtained as follows: Figure 4 As shown, Figure 4 In the graph, the horizontal axis represents time (unit: min), and the vertical axis represents the response value of the electrical signal (unit: mAu). Figure 4B is used to indicate the peak area (unit: mAu·min), and the peak area of ​​indole-3-acetic acid methyl ester of the tobacco bud inhibitor sample after the hot storage experiment is 64854.75008.

[0115] The storage decomposition rate of indole-3-acetic acid methyl ester in tobacco bud inhibitor is calculated as follows:

[0116] (67083.45590-64854.75008) / 67083.45590×100%=3.3%

[0117] That is, the storage decomposition rate (3.3%) of indole-3-acetic acid methyl ester of the tobacco sprout inhibitor in this embodiment is less than 5%, and the storage stability test is qualified.

[0118] 400 times, 200 times and 100 times dilutions of the tobacco bud inhibitor in this example, as well as flumetralin (CK1) and pendimethalin (CK2) were applied to the axillary buds of tobacco (the cup spraying method can be used for application), and the bud inhibitory effects are shown in Table 4.

[0119] Table 4 germination inhibition rate and germination inhibition effect

[0120]

[0121] As shown in Table 4, T1 represents a 400-fold dilution of tobacco bud inhibitor. T2 represents a 200-fold dilution of tobacco bud inhibitor. T3 represents a 100-fold dilution of tobacco bud inhibitor. CK1 represents a 200-fold dilution of 25% flumethalin emulsifiable concentrate. CK2 represents a 200-fold dilution of 33% pendimethalin emulsifiable concentrate.

[0122] As shown in Table 4, the data of germination inhibition rate and germination inhibition effect are mean ± standard error. After the new multiple range method (Duncan's new multiple range method) test, in the same column of Table 4, at the 0.05 level, the data marked a are significantly different from the data marked b and c, and are not significantly different from the data marked ab; the data marked b are significantly different from the data marked a and c, and are not significantly different from the data marked ab; the data marked c are significantly different from the data marked a, marked b, and marked ab; the data marked ab are significantly different from the data marked c, and are not significantly different from the data marked a and marked b.

[0123] As shown in Table 4, the number of tobacco live buds at 14 days, 28 days and 42 days after application was counted, and the bud inhibition rate and bud inhibition effect of T1, T2, T3, CK1 and CK2 were obtained. The bud inhibition rate can be calculated by the number of live buds above 2 cm, and the bud inhibition effect can be calculated by the weight of the buds. The specific bud inhibition rate and bud inhibition effect are as follows:

[0124] 14 days after application, the bud inhibition rates of T1, T2 and T3 were all 100.00%. The bud inhibition rates of the control groups CK1 and CK2 were 88.75% and 82.32% respectively. It can be seen that the bud inhibition rates of T1, T2 and T3 were all higher than those of CK1 and CK2. Among them, in the same column of Table 4, the difference between the data marked a and the data marked a and the data marked ab was not significant, the difference between the data marked a and the data marked b was significant, and the difference between the data marked ab and the data marked b was not significant.

[0125] 28 days after application, the bud inhibition rates of T1, T2 and T3 were 96.80%, 98.60% and 87.94% respectively, and the bud inhibition rates of the control groups CK1 and CK2 were 96.27% and 76.21% respectively. It can be seen that the bud inhibition rates of T1 and T2 were higher than those of CK1. The bud inhibition rates of T1, T2 and T3 were higher than those of CK2. Among them, in the same column of Table 4, the data marked a and the data marked a were not significantly different, and the data marked a and the data marked c were significantly different.

[0126] 42 days after application, the bud inhibition rates of T1, T2 and T3 were 97.05%, 96.53% and 81.83% respectively. The bud inhibition rates of the control groups CK1 and CK2 were 88.19% and 61.61% respectively. It can be seen that the bud inhibition rates of T1 and T2 are higher than those of CK1. The bud inhibition rates of T1, T2 and T3 are higher than those of CK2. Among them, in the same column of Table 4, the difference between the data marked a and the data marked a and the data marked ab is not significant, the difference between the data marked a and the data marked b and the data marked c is significant, the difference between the data marked b and the data marked ab is not significant, and the difference between the data marked c and the data marked ab is significant.

[0127] 42 days after application, the sprout inhibition effects of T1, T2 and T3 were 99.34%, 96.87% and 91.74% respectively. The sprout inhibition effects of the control groups CK1 and CK2 were 76.41% and 57.17% respectively. It can be seen that the sprout inhibition effects of T1, T2 and T3 are all higher than those of CK1 and CK2. Among them, in the same column of Table 4, the difference between the data marked a and the data marked a and the data marked ab is not significant, the difference between the data marked a and the data marked b and the data marked c is significant, the difference between the data marked b and the data marked ab is not significant, and the difference between the data marked c and the data marked ab is significant.

[0128] Therefore, the tobacco sprout inhibitor of this embodiment can achieve effective sprout inhibition.

[0129] Example 2

[0130] The tobacco sprout suppressant in this embodiment includes the following components by mass percentage: 5% indole-3-acetic acid methyl ester, 40% acetone solvent, 8% emulsifier, 1% n-pentanol, 3% glycerol, and 43% deionized water. Among them, the emulsifier includes Agricultural Ru No. 400.

[0131] The preparation method of the tobacco sprout inhibitor in this example is the same as that in Example 1.

[0132] The tobacco bud inhibitor in this embodiment can not only achieve effective bud inhibition, but also reduce drug residues, and has qualified appearance stability and qualified cold storage stability.

[0133] Example 3

[0134] The tobacco sprout suppressant in this embodiment includes the following components by mass percentage: 15% indole-3-acetic acid methyl ester, 55% acetone solvent, 8% emulsifier, 3% isopropanol, 2% propylene glycol, and 17% deionized water. Among them, the emulsifier includes Agricultural Ru No. 500.

[0135] The preparation method of the tobacco sprout inhibitor in this example is the same as that in Example 1.

[0136] The tobacco bud inhibitor in this embodiment can not only achieve effective bud inhibition, but also reduce drug residues, and has qualified appearance stability, qualified emulsion stability, qualified cold storage stability, and a pH value of 6.87.

[0137] Example 4

[0138] The tobacco sprout suppressant in this embodiment includes the following components by mass percentage: 20% indole-3-acetic acid methyl ester, 60% acetone solvent, 12% emulsifier, 2% n-butanol, 1% polyethylene glycol, and 5% deionized water. Among them, the emulsifier includes Agricultural Ru No. 1602.

[0139] The preparation method of the tobacco sprout inhibitor in this example is the same as that in Example 1.

[0140] The tobacco bud inhibitor in this embodiment can not only achieve effective bud inhibition, but also reduce drug residues, and has qualified appearance stability, qualified emulsion stability, and qualified cold storage stability.

[0141] Example 5

[0142] The tobacco sprout suppressant in this embodiment includes the following components by mass percentage: 10% indole-3-acetic acid methyl ester, 50% acetone solvent, 20% emulsifier, 2% n-butanol, 4% ethylene glycol, and 14% deionized water. Among them, the emulsifier includes Agricultural Ru No. 600.

[0143] The preparation method of the tobacco sprout inhibitor in this example is the same as that in Example 1.

[0144] The tobacco bud inhibitor in this embodiment can not only achieve effective bud inhibition, but also reduce drug residues, and has qualified appearance stability, a level of emulsion dispersibility of 2, qualified cold storage stability, qualified hot storage stability, and a pH value of 6.93.

[0145] Example 6

[0146] The tobacco bud suppressant in this embodiment includes the following components by mass percentage: 10% indole-3-acetic acid methyl ester, 50% acetone solvent, 12% emulsifier, 3% n-butanol, 5% ethylene glycol, and 20% deionized water. Among them, the emulsifier includes Nongru No. 500 and Nongru No. 1602, and the mass ratio of Nongru No. 1602 to Nongru No. 500 is 1:1.

[0147] The preparation method of the tobacco sprout inhibitor in this example is the same as that in Example 1.

[0148] The tobacco bud inhibitor in this embodiment can not only achieve effective bud inhibition, but also reduce drug residues, and has qualified appearance stability, a level of emulsion dispersibility of 2, qualified cold storage stability, qualified hot storage stability, and a pH value of 6.85.

[0149] Example 7

[0150] The tobacco bud suppressant in this embodiment includes the following components by mass percentage: 10% indole-3-acetic acid methyl ester, 50% acetone solvent, 12% emulsifier, 5% n-butanol, 3% ethylene glycol, and 20% deionized water. Among them, the emulsifier includes Nongru No. 500 and Nongru No. 1602, and the mass ratio of Nongru No. 1602 to Nongru No. 500 is 1:1.

[0151] The preparation method of the tobacco sprout inhibitor in this example is the same as that in Example 1.

[0152] The tobacco bud inhibitor in this embodiment can not only achieve effective bud inhibition, but also reduce drug residues, and has qualified appearance stability, qualified emulsion stability, a level of emulsion dispersibility of 2, qualified cold storage stability, qualified hot storage stability, and a pH value of 6.95.

[0153] Example 8

[0154] The tobacco bud suppressant in this embodiment includes the following components by mass percentage: 8% indole-3-acetic acid methyl ester, 50% acetone solvent, 15% emulsifier, 2% n-butanol, 5% ethylene glycol, and 20% deionized water. Among them, the emulsifier includes Nongru No. 500 and Nongru No. 1602, and the mass ratio of Nongru No. 1602 to Nongru No. 500 is 1:1.

[0155] The preparation method of the tobacco sprout inhibitor in this example is the same as that in Example 1.

[0156] The tobacco bud inhibitor in this embodiment can not only achieve effective bud inhibition, but also reduce drug residues, and has qualified appearance stability, qualified emulsion stability, and qualified cold storage stability.

[0157] Example 9

[0158] The tobacco bud suppressant in this embodiment includes the following components by mass percentage: 10% indole-3-acetic acid methyl ester, 50% acetone solvent, 15% emulsifier, 3% n-butanol, 3% ethylene glycol, and 19% deionized water. Among them, the emulsifier includes Nongru No. 500 and Nongru No. 1602, and the mass ratio of Nongru No. 1602 to Nongru No. 500 is 1:1.

[0159] The preparation method of the tobacco sprout inhibitor in this example is the same as that in Example 1.

[0160] The tobacco sprout inhibitor in this embodiment can not only achieve effective sprout inhibition, but also reduce drug residues, and has satisfactory cold storage stability.

[0161] Next, 200 times the solution of the tobacco bud inhibitor in Examples 1 to 9, 200 times the solution of pendimethalin, 200 times the solution of butalin, and 200 times the solution of flumetrazone were applied to tobacco, respectively, and the half-life and 7-day residue on the tobacco were observed. The results are shown in Table 5.

[0162] It should be noted that the half-life refers to the time required for the amount of a certain substance (such as pesticides, radioactive elements) to be reduced to half of the initial amount in the environment or in an organism. In the application, the half-life specifically refers to: the time required for the 200-fold solution of the tobacco bud inhibitor in Examples 1 to 9, the 200-fold solution of pendimethalin, the 200-fold solution of butalin, and the 200-fold solution of flumetralin to be reduced to half of the initial amount on the tobacco after taking the same initial amount and applying them to different tobaccos respectively. Among them, multiple samples of the same initial amount of each bud inhibitor can be prepared and applied to different tobaccos respectively to improve the accuracy of the results.

[0163] It should be noted that the 7-day residual refers to the residual amount of the bud inhibitor in tobacco 7 days after the bud inhibitor is applied to the tobacco.

[0164] Table 5 Half-life and residual amount of different bud inhibitors on tobacco

[0165] deal with Half-life (days) 7-day residual amount (mg / kg) Example 1 1.63～4.32 0.04 Example 2 1.78～5.08 0.34 Example 3 1.70～4.99 0.22 Example 4 2.05～5.15 0.13 Example 5 1.97～4.83 0.31 Example 6 2.14～5.07 0.34 Example 7 1.58～4.89 0.28 Example 8 2.49～5.14 0.08 Example 9 1.69～4.44 0.05 Pendimethalin 2.56～5.97 0.40 Butyl 5.2～12.2 0.44 Flumetholone 3.3～5.2 1.53

[0166] As shown in Table 5, when the 200-fold solution of the tobacco bud inhibitor in Example 1 is applied to tobacco, the half-life is 1.63 to 4.32 days, and the residual amount in 7 days is 0.04 mg / kg. When the 200-fold solution of the tobacco bud inhibitor in Example 2 is applied to tobacco, the half-life is 1.78 to 5.08 days, and the residual amount in 7 days is 0.34 mg / kg. When the 200-fold solution of the tobacco bud inhibitor in Example 3 is applied to tobacco, the half-life is 1.70 to 4.99 days, and the residual amount in 7 days is 0.22 mg / kg. When the 200-fold solution of the tobacco bud inhibitor in Example 4 is applied to tobacco, the half-life is 2.05 to 5.15 days, and the residual amount in 7 days is 0.13 mg / kg. When the 200-fold solution of the tobacco bud inhibitor in Example 5 is applied to tobacco, the half-life is 1.97 to 4.83 days, and the residual amount in 7 days is 0.31 mg / kg. 200 times the solution of the tobacco bud inhibitor in Example 6 was applied to tobacco, and the half-life was 2.14 to 5.07 days, and the 7-day residual amount was 0.34 mg / kg. 200 times the solution of the tobacco bud inhibitor in Example 7 was applied to tobacco, and the half-life was 1.58 to 4.89 days, and the 7-day residual amount was 0.28 mg / kg. 200 times the solution of the tobacco bud inhibitor in Example 8 was applied to tobacco, and the half-life was 2.49 to 5.14 days, and the 7-day residual amount was 0.08 mg / kg. 200 times the solution of the tobacco bud inhibitor in Example 9 was applied to tobacco, and the half-life was 1.69 to 4.44 days, and the 7-day residual amount was 0.05 mg / kg.

[0167] As shown in Table 5, when a 200-fold dilution of pendimethalin is applied to tobacco, the half-life is 2.56 to 5.97 days, and the residual amount after 7 days is 0.40 mg / kg. When a 200-fold dilution of sec-butyl is applied to tobacco, the half-life is 5.2 to 12.2 days, and the residual amount after 7 days is 0.44 mg / kg. When a 200-fold dilution of flumetralin is applied to tobacco, the half-life is 3.3 to 5.2 days, and the residual amount after 7 days is 1.53 mg / kg.

[0168] It can be seen that the half-life and 7-day residual amount of the tobacco sprout inhibitors in Examples 1 to 9 are better than those of the sprout inhibitors pendimethalin, butralin and flumethalin. Therefore, the tobacco sprout inhibitor of the present application has the effects of fast degradation, easy degradation and low residue.

[0169] Below, specific comparative examples are given to further illustrate the embodiments of the present disclosure.

[0170] Comparative Example 1

[0171] The sprout inhibitor includes the following components: 10% indole-3-acetic acid, 50% acetone solvent, 15% emulsifier, 4% n-butanol, 4% ethylene glycol, and 17% deionized water. The emulsifier includes Nong Ru No. 500 and Nong Ru No. 1602, and the mass ratio of Nong Ru No. 1602 to Nong Ru No. 500 is 1:1.

[0172] The difference between Comparative Example 1 and Example 1 is that the indole-3-acetic acid methyl ester in Example 1 is replaced by indole-3-acetic acid. The other components are the same as those in Example 1.

[0173] 200 times the solution of the bud inhibitor in Comparative Example 1 and 200 times the solution of the tobacco bud inhibitor in Example 1 were applied to the axillary buds of tobacco, respectively, and the bud inhibition rate and bud inhibition effect were obtained as shown in Table 6, and the half-life and 7-day residue were obtained as shown in Table 7.

[0174] Comparative Example 2

[0175] The sprout inhibitor includes the following components: 10% indolebutyric acid, 50% acetone solvent, 15% emulsifier, 4% n-butanol, 4% ethylene glycol, and 17% deionized water. The emulsifier includes Nong Ru No. 500 and Nong Ru No. 1602, and the mass ratio of Nong Ru No. 1602 to Nong Ru No. 500 is 1:1.

[0176] The difference between Comparative Example 2 and Example 1 is that the indole-3-acetic acid methyl ester in Example 1 is replaced by indolebutyric acid. The other components are the same as those in Example 1.

[0177] 200 times the solution of the bud inhibitor in Comparative Example 2 and 200 times the solution of the tobacco bud inhibitor in Example 1 were applied to the axillary buds of tobacco, respectively, and the bud inhibition rate and bud inhibition effect were obtained as shown in Table 6, and the half-life and 7-day residue were obtained as shown in Table 7.

[0178] Table 6 germination inhibition rate and germination inhibition effect

[0179]

[0180] As shown in Table 6, T2 represents a 200-fold solution of the tobacco bud inhibitor in Example 1. T4 represents a 200-fold solution of the bud inhibitor in Comparative Example 1. T5 represents a 200-fold solution of the bud inhibitor in Comparative Example 2. The specific bud inhibition rates and bud inhibition effects are as follows:

[0181] 14 days after application, the germination inhibition rate of T2 was 100.00%. The germination inhibition rate of T4 was 95.18%. The germination inhibition rate of T5 was 85.54%. It can be seen that the germination inhibition rate of T2 was higher than that of T4 and T5. Among them, in the same column of Table 6, the data marked a, the data marked b, and the data marked c were significantly different.

[0182] 28 days after application, the bud inhibition rate of T2 was 98.60%. The bud inhibition rate of T4 was 47.39%. The bud inhibition rate of T5 was 47.76%. It can be seen that the bud inhibition rate of T2 is higher than that of T4 and T5. Among them, in the same column of Table 6, the difference between the data marked a and the data marked b is significant, the difference between the data marked a and the data marked bc is significant, and the difference between the data marked b and the data marked bc is not significant.

[0183] 42 days after application, the bud inhibition rate of T2 was 96.53%. The bud inhibition rate of T4 was 43.57%. The bud inhibition rate of T5 was 35.88%. It can be seen that the bud inhibition rate of T2 is higher than that of T4 and T5. Among them, in the same column of Table 6, the difference between the data marked a and the data marked b is significant, the difference between the data marked a and the data marked bc is significant, and the difference between the data marked b and the data marked bc is not significant.

[0184] 42 days after application, the bud inhibition effect of T2 was 96.87%. The bud inhibition effect of T4 was 21.16%. The bud inhibition effect of T5 was 20.00%. It can be seen that the bud inhibition effect of T2 is higher than that of T4 and T5. Among them, in the same column of Table 6, the difference between the data marked a and the data marked b is significant, the difference between the data marked a and the data marked bc is significant, and the difference between the data marked b and the data marked bc is not significant.

[0185] Therefore, the tobacco sprout inhibitor of Example 1 can achieve effective sprout inhibition.

[0186] Table 7 Half-life and 7-day residual content

[0187] deal with Half-life (days) 7-day residual amount (mg / kg) Example 1 1.63～4.32 0.04 Comparative Example 1 2.85～5.67 0.88 Comparative Example 2 2.97～6.05 1.21

[0188] As shown in Table 7, when 200 times the amount of the tobacco bud inhibitor in Example 1 was applied to tobacco, the half-life was 1.63 to 4.32 days, and the residual amount after 7 days was 0.04 mg / kg. When 200 times the amount of the bud inhibitor in Comparative Example 1 was applied to tobacco, the half-life was 2.85 to 5.67 days, and the residual amount after 7 days was 0.88 mg / kg. When 200 times the amount of the bud inhibitor in Comparative Example 2 was applied to tobacco, the half-life was 2.97 to 6.05 days, and the residual amount after 7 days was 1.21 mg / kg.

[0189] It can be seen that the half-life and 7-day residual amount of the tobacco sprout inhibitor of Example 1 are better than those of Comparative Example 1 and Comparative Example 2. Therefore, the tobacco sprout inhibitor of Example 1 has the effects of fast degradation, easy degradation and low residual.

[0190] The above are only preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present application shall be included in the protection scope of the present application. The above are only preferred implementations of the present application. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present application, and these improvements and variations should also be regarded as the protection scope of the present application.

Claims

1. A tobacco sprout inhibitor, characterized in that: The tobacco bud inhibitor comprises the following components by mass percentage: Indole-3-acetic acid methyl ester 5% to 20%, ketone solvent 35% to 70%, emulsifier 8% to 20%, and the rest is deionized water.

2. The tobacco bud inhibitor according to claim 1, characterized in that The ketone solvent includes one or more of acetone, isophorone and cyclohexanone.

3. The tobacco sprout inhibitor according to claim 1, characterized in that The emulsifier includes one or more of Nong Ru No. 400, Nong Ru No. 500, Nong Ru No. 600 and Nong Ru No. 1602.

4. The tobacco bud inhibitor according to any one of claims 1 to 3, characterized in that The tobacco bud inhibitor further comprises the following components by mass percentage: Co-surfactant 1% to 5%, and / or, antifreeze agent 1% to 5%.

5. The tobacco sprout suppressant according to claim 4, characterized in that The co-surfactant includes one or more of ethanol, n-propanol, isopropanol, n-butanol, isobutanol, n-pentanol, isopentanol, 1-hexanol, 2-hexanol, 1-octanol and 2-octanol.

6. The tobacco sprout inhibitor according to claim 4, characterized in that The antifreeze agent includes one or more of ethylene glycol, propylene glycol, glycerol and polyethylene glycol.

7. A method for preparing a tobacco sprout inhibitor, for preparing the tobacco sprout inhibitor as claimed in any one of claims 1 to 6, characterized in that: The preparation method comprises the following steps: dissolving the indole-3-acetic acid methyl ester in the ketone solvent to form a solution; Adding the emulsifier into the solution and stirring evenly to form an oil phase; The deionized water is added into the oil phase and stirred according to preset stirring parameters to obtain the tobacco sprout inhibitor.

8. The method for preparing the tobacco sprout inhibitor according to claim 7, characterized in that: The preset stirring parameters include: Stirring speed: 10000r / min to 12000r / min; and / or, Stirring time: 1min to 3min.

9. The method for preparing the tobacco sprout inhibitor according to claim 7 or 8, characterized in that: In the step of adding the emulsifier to the solution, a co-surfactant is also added to the solution.

10. The method for preparing the tobacco sprout inhibitor according to claim 7 or 8, characterized in that: In the step of adding the deionized water into the oil phase, an antifreeze agent is also added into the oil phase.

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