Bactericidal composition containing plant source activity and bactericide

Abstract

The invention belongs to the technical field of bactericides, and particularly relates to a bactericidal composition containing plant source activity and a bactericide. The invention relates to a bactericidal composition, the bactericidal composition is formed by compounding a plant source active component and fluopyram, and the plant source active component is berberine or physcion. The botanical active ingredients berberine and physcion are respectively compounded with fluopyram, so that compared with a single ingredient, the bactericidal composition has the advantages that the control effect on fungal diseases and nematode diseases can be improved, and the propagation of the diseases can be effectively controlled; meanwhile, accumulation and development of target drug resistance can be delayed, and the service life of the pesticide is prolonged; secondly, the existing medicament can be fully utilized, the cost of developing a novel medicament is reduced, and the composition has very important significance on comprehensive treatment of crop diseases and insect pests.

Description

Technical Field

[0001] The invention belongs to the technical field of fungicides, and in particular relates to a fungicide composition containing plant-derived activity and a fungicide. Background Art

[0002] Berberine, also known as berberine, is an isoquinoline alkaloid extracted from plants such as Coptis chinensis. Clinically, berberine has a broad-spectrum antibacterial effect and has inhibitory effects on a variety of Gram-positive and Gram-negative bacteria in vitro, especially on hemolytic Streptococcus, Staphylococcus aureus and Vibrio cholerae. In addition, berberine also has multiple pharmacological activities such as anti-inflammatory, anti-cancer, hypoglycemic and lipid metabolism. In terms of agricultural disease control, berberine, as a plant-derived alkaloid compound, is mainly used to control a variety of diseases such as fungi, bacteria and viruses, and has a good preventive effect on pests such as aphids, borers, red spiders and rice planthoppers. The prior art discloses that berberine exhibits significant nematicidal activity against southern root-knot nematodes. Secondly, the prior art also discloses that the combination of tolfenpyrad and berberine has good preventive effect against crop root-knot nematodes, pine wood nematodes, cyst nematodes and stem nematodes, and can provide a synergistic effect for the prevention and control of crop nematodes.

[0003] Physcion, chemically named 1,8-dihydroxy-3-methoxy-6-methylanthraquinone, is a secondary metabolite extracted from the roots and stems of the Chinese herbal medicine rhubarb. It has shown wide application potential in the fields of medicine and agriculture. Clinically, physcion has inhibitory effects on a variety of bacteria including Escherichia coli, Pseudomonas aeruginosa, Streptococcus and Shigella dysenteriae, and has a significant inhibitory effect on human cervical cancer Helad cells. In terms of agricultural pest control, physcion is a plant-derived fungicide, mainly used to control fungal plant diseases. It interferes with the biosynthesis of chitin in the cell wall of pathogenic fungi, causing the germ tube and mycelium to swell and rupture locally, leaking cell contents, and leading to the death of pathogens. It has a good control effect on powdery mildew, downy mildew and anthracnose.

[0004] Fluopyram is a succinate dehydrogenase inhibitor (SDHI) fungicide with a bridged heterocyclic amide structure. Its mechanism of action is to inhibit the activity of succinate dehydrogenase in the respiratory electron transport chain and tricarboxylic acid cycle of pathogenic bacteria, interfere with respiration, hinder energy production, inhibit pathogenic bacteria spore germination, tooth tube growth, mycelium growth and spore formation, and lead to pathogen death; in addition, fluopyram can selectively inhibit the respiratory chain complex II in the mitochondria of nematodes, interfere with its respiration, and cause the energy ATP in nematode cells to be quickly exhausted and die. Incident, fluopyram is a fungicide and nematode compound with good market prospects. The International Fungicide Resistance Action Committee FRAC divides SDHI fungicides into 12 groups based on chemical structure, among which fluopyram belongs to the pyridylethylbenzamide group. However, as a fungicide, fluopyram is widely used as a single ingredient for a long time, which is easy to cause the accumulation and development of target resistance, resulting in a reduction in its control effect on the target.

[0005] In agricultural production, there are many pesticides used to prevent and control plant diseases and insect pests, but due to their long-term unscientific use of single ingredients, they have a high degree of drug resistance. Researching and developing new compounds takes a long time and is costly. Compounding different active ingredients and screening for combinations with synergistic effects can not only improve the prevention and control effect of diseases, but also make full use of existing pesticides. It is an effective method for integrated pest control. Summary of the invention

[0006] The object of the present invention is to provide a fungicide composition and fungicide containing plant-derived activity, which can delay the accumulation and development of target drug resistance, improve the control effect, and make full use of existing drugs.

[0007] The present invention provides the following technical solutions:

[0008] A fungicide composition is prepared by compounding a plant-derived active ingredient and fluopyram, wherein the plant-derived active ingredient is berberine or physcion methyl ether.

[0009] The effect is better, and the mass ratio of berberine to fluopyram is 1-6:18-1.

[0010] The effect is better, and the mass ratio of physaccharin methyl ether to fluopyram is 1-20:20-1.

[0011] The invention also provides application of the bactericidal composition in preventing and controlling plant fungal diseases.

[0012] More specifically, the plant fungal diseases include pepper anthracnose.

[0013] The present invention also provides the use of the bactericidal composition in preventing and treating plant root-knot nematode disease, wherein the bactericidal composition is compounded with berberine and fluopyram.

[0014] A bactericide, comprising the bactericidal composition.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention compounds the plant-derived active ingredients berberine and physcion with fluopyramid respectively. Compared with single ingredients, the control effect on fungal diseases such as pepper anthracnose and nematode diseases such as southern root-knot nematodes can be improved, and the spread of diseases can be effectively controlled; at the same time, the accumulation and development of target drug resistance can be delayed, and the service life of the agent can be extended; secondly, existing agents can be fully utilized, and the cost of developing new agents can be reduced, which is of great significance to the comprehensive control of crop diseases and insect pests. DETAILED DESCRIPTION

[0017] The technical solution of the present invention is further described below in conjunction with the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by technical personnel in the field without making creative work are within the scope of protection of the present invention.

[0018] Example 1: Combined effect of compounded agents on fungal diseases

[0019] 1.1 Test strains

[0020] Diseased pepper samples were collected, separated, purified and identified in the laboratory, and it was determined that the pepper anthracnose was caused by Colletotrichum gloeosporioides.

[0021] 1.2 Test agents

[0022] 98% berberine (CAS: 2086-83-1), 98% physaccharin methyl ether (CAS: 521-61-9), 96% fluopyram (Registration No.: PD20242134)

[0023] 1.3 Test method (refer to "NY / T 1156.2-2006 Pesticide Indoor Bioassay Test Guidelines Fungicides Part 2: Inhibition of Pathogenic Fungal Mycelial Growth Test Plate Method")

[0024] The test agent was dissolved in dimethyl sulfoxide and then diluted with 0.05% Tween-80 aqueous solution to obtain a single-dose mother solution; multiple groups of ratios were set, and each single dose and each group of mixed ratios were diluted with 0.05% Tween-80 aqueous solution into 5 mass concentration gradients. The PDA culture medium cooled to about 53°C and the drug solution were mixed in a ratio of 9:1, shaken thoroughly, poured into a culture dish with a diameter of 9 cm, 12 mL was poured into each dish, cooled, and a drug-containing plate was made. In the same way, 0.05% Tween-80 aqueous solution and PDA culture medium were mixed to make a drug-free plate, which was used as a blank control. Each treatment was repeated 3 times, and each repeat had 3 plates.

[0025] The test strain was inoculated on PDA medium and cultured in a constant temperature incubator at 25℃ for 4 days. A 6mm diameter bacterial cake was punched along the edge of the test strain colony with a hole puncher, and one bacterial cake was inoculated in the center of the drug-containing plate and the drug-free plate, respectively. The plates were covered with lids and cultured in a constant temperature incubator at 25℃. When the diameter of the blank control colony grew to 2 / 3 of the diameter of the culture dish, the diameter of the colony was measured by the cross method, and the mycelial growth inhibition rate of each treatment and concentration on the target bacteria was calculated.

[0026]

[0027] 1.4 Data Analysis

[0028] DPS software was used for data statistical analysis. The logarithmic value of the concentration of the agent was taken as x and the corresponding probability value of mycelium growth inhibition rate was taken as y for linear regression. The toxicity regression equation and the toxicity EC value of the agent to the target pathogen were obtained. 50 The co-toxicity coefficient (CTC) was calculated using the Sun Yunpei method, and the synergistic effect of the agent was evaluated based on the calculated co-toxicity coefficient (CTC). CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 1-2.

[0029] Table 1 The combined effect of berberine and fluopyram on pepper anthracnose pathogen Colletotrichum gloeosporioides

[0030]

[0031] As can be seen from Table 1, when berberine and fluopyram are compounded in a mass ratio range of 1-6:18-1, the compound combination exhibits a synergistic effect on pepper anthracnose pathogen Colletotrichum gloeosporioides, indicating that compounding berberine and fluopyram in the present invention can improve the control effect on pepper anthracnose.

[0032] Table 2 The combined effect of physaccharin methyl ether and fluopyram on pepper anthracnose pathogen Colletotrichum gloeosporioides

[0033]

[0034]

[0035] It can be seen from Table 2 that when physaccharin methyl ether and fluopyram are compounded in a mass ratio range of 1-20:20-1, the compound combination exhibits a synergistic effect on pepper anthracnose pathogen Colletotrichum gloeosporioides, indicating that compounding physaccharin methyl ether and fluopyram in the present invention can improve the control effect on pepper anthracnose.

[0036] Example 2: Combined effect of compounded agents on root-knot nematodes

[0037] 2.1 Test nematodes

[0038] The eggs of southern root-knot nematode (Meloidogyne incognita) were picked from the roots of peppers, incubated at 25°C, and the second-instar larvae of the same age were obtained. The root-knot nematodes were resuspended in water to 150 heads / mL for later use.

[0039] 2.2 Test agents

[0040] 98% berberine (CAS: 2086-83-1), 96% fluopyram (Registration No.: PD20242134)

[0041] 2.3 Test method (refer to "NY / T 1833.1-2009 Guidelines for Indoor Bioassay Tests of Pesticides - Nematicides Part 1: Test for Inhibition of Plant Pathogenic Nematodes - Insect Immersion Method")

[0042] The test agent was dissolved in dimethyl sulfoxide and then diluted with 0.05% Tween-80 aqueous solution to obtain a single-dose mother solution; multiple groups of ratios were set, and each single agent and each group of mixed agents were diluted with 0.05% Tween-80 aqueous solution to 5 mass concentration gradients. 3mL of the drug solution and 3mL of the root-knot nematode suspension were mixed in equal amounts, and the mixture was dropped into the small holes of a 24-well biochemical test plate, covered, and cultured at a constant temperature of 25℃ for 36h, with 5 replicates per treatment. The treatment with 0.05% Tween-80 aqueous solution was set as a blank control.

[0043] Take 1 mL of the mixture from each treatment and observe the nematode death under a dissecting microscope. Observe 120 nematodes per repetition and record the total number of nematodes investigated and the number of dead nematodes. Dead nematodes are stiff and cannot bend or move when touched with a hairpin or bamboo needle. Calculate the mortality rate and adjusted mortality rate of each treatment based on the survey results.

[0044]

[0045] In the above formula: P--mortality rate, unit is %; K--number of dead insects; N--total number of insects treated.

[0046]

[0047] In the above formula: P1--corrected mortality rate, unit is %; P t --Treatment mortality rate, in %; P0--blank control mortality rate, in %.

[0048] 1.4 Data Analysis

[0049] DPS software was used for data statistical analysis. The logarithmic value of the concentration of the pesticide was taken as x and the corresponding corrected mortality probability value was taken as y for linear regression. The toxicity regression equation and the toxicity LC value of the pesticide to root-knot nematodes were obtained. 50 The co-toxicity coefficient (CTC) was calculated by Sun Yunpei method, and the synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC). CTC≤80 was antagonistic effect, 80<CTC<120 was additive effect, and CTC≥120 was synergistic effect. The results are shown in Table 3.

[0050] Table 3 The combined effect of berberine and fluopyram on pepper anthracnose pathogen Colletotrichum gloeosporioides

[0051]

[0052]

[0053] It can be seen from Table 3 that when berberine and fluopyram are compounded in a mass ratio range of 1-6:18-1, the compound combination exhibits a synergistic effect on southern root-knot nematodes, indicating that compounding berberine and fluopyram in the present invention can improve the control effect on southern root-knot nematodes.

Claims

1. A bactericidal composition, characterized in that: The bactericidal composition is compounded by plant-derived active ingredients and fluopyram, and the plant-derived active ingredients are berberine or physaccharin methyl ether.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of berberine to fluopyram is 1-6:18-1.

3. The bactericidal composition according to claim 1, characterized in that The mass ratio of physaccharin methyl ether to fluopyram is 1-20:20-1.

4. Use of the fungicidal composition according to claim 1 in preventing and controlling plant fungal diseases.

5. The use according to claim 4, characterized in that: The plant fungal diseases include pepper anthracnose.

6. Use of the fungicide composition according to claim 2 in preventing and controlling plant root-knot nematode disease.

7. A bactericide, characterized in that: The bactericide comprises the bactericidal composition according to claim 1.

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