Solenopsis invicta bait as well as preparation method and application thereof

Abstract

The invention belongs to the field of solenopsis invicta prevention and control, and particularly relates to a bait for preventing and controlling solenopsis invicta as well as a preparation method and application thereof. The composition is prepared from indoxacarb and spinosad, and the mass ratio of spinosad to indoxacarb is (5: 1)-(1: 5). The bait provided by the invention adopts a puffing process, is light in weight, is adverse to the environment, and does not affect the attraction effect on the solenopsis invicta, and the invention also provides a preparation method of the bait.

Description

Technical Field

[0001] The invention belongs to the field of red fire ant prevention and control, and specifically relates to a bait for preventing and controlling red fire ants, and a preparation method and application thereof. Background Art

[0002] Red fire ants belong to the genus Solenopsis of the family Formicidae in the order Hymenoptera. They are an invasive species. Due to the lack of natural enemies, red fire ants invade quickly and cause great harm. In order to effectively prevent and control the reproduction and spread of red fire ants, the development of an efficient red fire ant bait is the focus of current research.

[0003] The existing prevention and control measures are mainly chemical baits. Bait killing red fire ants has the advantages of simple operation, safety, and target determination, and can achieve the effect of preventing and controlling red fire ants. However, the existing baits are not attractive enough to red fire ants, and red fire ants often refuse to eat. The attractiveness of red fire ant baits is an urgent problem to be solved.

[0004] The active ingredient of conventional red fire ant control is a single indoxacarb, which has played a certain role in promoting the resistance of red fire ants. It is also an urgent issue to enrich the red fire ant control agents and slow down the occurrence of red fire ant resistance. Therefore, it is of great significance to develop compound baits. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a bait for controlling red fire ants and a preparation method and application thereof in view of the deficiencies in the prior art.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] A composition for preventing and controlling red imported fire ants comprises indoxacarb and spinosad, wherein the mass ratio of indoxacarb to spinosad is 5:1 to 1:5.

[0008] An application of the composition for preventing and controlling red fire ants, and an application of the composition for preventing and controlling red fire ants in preparing red fire ant baits.

[0009] A red fire ant bait, comprising a bait, the active ingredient and an attractant ingredient, wherein the attractant ingredient is farnesene and / or 2-ethyl-3,6-dimethylpyrazine.

[0010] The red fire ant bait comprises, by weight percentage, 0.05% to 0.2% active ingredient, 0.01% to 0.02% attractant ingredient, and the remainder is bait.

[0011] Preferably, the mass ratio of the active ingredients spinosad and indoxacarb is 1:2.5.

[0012] The attractant component is farnesene and 2-ethyl-3,6-dimethylpyrazine in a mass ratio of 2:1 to 1:2, preferably 1:1.5.

[0013] The farnesene is (Z,E)-α-farnesene.

[0014] The bait is one or more of corn grits, peanut crumbs, meat powder, and yeast glucan.

[0015] The red fire ant bait also contains a preservative, wherein the amount of the preservative added accounts for 0.3% to 0.5% of the mass of the red fire ant bait.

[0016] The preservative is benzoic acid and / or citric acid.

[0017] An application of the red fire ant bait, and application of the red fire ant bait in the field of red fire ant prevention and control.

[0018] The red fire ant bait is prepared as expanded particles.

[0019] The raw materials are fully mixed according to the above percentages, and then ultra-finely ground. After the grinding, water is added and the powder is expanded and granulated by an extruder to obtain expanded granular red fire ant bait.

[0020] When the bait is used to control red fire ants, 20 to 25 g of the bait is spread in each nest. Compared with the prior art, the bait has the following characteristics:

[0021] The bait of the present invention has good attraction to red fire ants and improves the prevention effect; the compounding of two insecticides with different action mechanisms and the addition of specific auxiliary materials can also delay the generation of resistance, thus providing a new method for preventing and controlling red fire ants, delaying resistance and enhancing attraction; at the same time, the bait adopts a puffing process, has a light texture, and has no effect on the attraction effect to red fire ants in an unfavorable environment, and improves the ability of the bait to resist rain erosion, thereby solving the problems that conventional baits are difficult to carry and are greatly affected by the environment DETAILED DESCRIPTION

[0022] The specific implementation methods of the technical solution of the present invention are described in detail below, but the present invention is not limited to the following description. The present invention is explained below in conjunction with specific embodiments.

[0023] The following embodiments and comparative examples are used to prepare the expanded bait granules by fully mixing the raw materials according to the above percentages, and then ultrafine grinding them. After grinding, water is added and the powder is expanded and granulated by an expander to obtain expanded granular bait.

[0024] The ultrafine grinding is carried out by using a jet mill at a feeding speed of 50g to 100g per minute and an air pressure of 0.4mPa to 0.6mPa;

[0025] The puffing condition of the puffing machine is a temperature of about 100° C. and a twin-screw puffing machine for extrusion.

[0026] Preparation of blank red fire ant bait:

[0027] Example 1

[0028] The blank red fire ant bait was 1000g of corn grits.

[0029] Embodiment 2:

[0030] The blank red fire ant bait is 100g of crushed peanuts, supplemented with corn grits to 1000g.

[0031] Embodiment 3:

[0032] The blank red fire ant bait is 100g of crushed peanuts, 100g of meat powder, and corn grits to make up to 1000g.

[0033] Embodiment 4:

[0034] The blank red fire ant bait is composed of 100g crushed peanuts, 100g meat powder, 100g yeast glucan, and corn grits to make up to 1000g.

[0035] Embodiment 5:

[0036] The blank red fire ant bait is (Z,E)-α-farnesene 0.08g, 2-ethyl-3,6-dimethylpyrazine 0.12g, crushed peanuts 100g, meat powder 100g, yeast glucan 100g, and corn grits are added to 1000g.

[0037] Embodiment 6:

[0038] The blank red fire ant bait is (Z,E)-α-farnesene 0.04g, 2-ethyl-3,6-dimethylpyrazine 0.06g, crushed peanuts 100g, meat powder 100g, yeast glucan 100g, and corn grits are supplemented to 1000g.

[0039] Embodiment 7:

[0040] The blank red fire ant bait is (Z,E)-α-farnesene 0.12g, 2-ethyl-3,6-dimethylpyrazine 0.08g, crushed peanuts 100g, meat powder 100g, yeast glucan 100g, and corn grits are added to 1000g.

[0041] Embodiment 8:

[0042] The blank red fire ant bait is (Z,E)-α-farnesene 0.06g, 2-ethyl-3,6-dimethylpyrazine 0.04g, crushed peanuts 100g, meat powder 100g, yeast glucan 100g, and corn grits are supplemented to 1000g.

[0043] Comparative Example 1:

[0044] The blank red fire ant bait is (E,E)-α-farnesene 0.06g, 2-ethyl-3,6-dimethylpyrazine 0.04g, crushed peanuts 100g, meat powder 100g, yeast glucan 100g, and corn grits are supplemented to 1000g.

[0045] Verification test: Attraction effect test

[0046] (1) Test subjects: Red imported fire ants

[0047] (2) Test method: Same as field test method.

[0048] (3) Test agent:

[0049] Examples 1 to 8 and Comparative Example 1.

[0050] (4) Statistics:

[0051] Table 1 Results of several treatments for attracting red fire ants

[0052] Treatment Group 24h Red Fire Ant Removal Rate Example 1 85％ Example 2 86％ Example 3 92％ Example 4 90％ Example 5 95％ Example 6 96％ Example 7 94％ Example 8 95％ Comparative Example 1 75％

[0053] The test results show that common food baits can attract red fire ants, with a red fire ant carrying rate of more than 80%; the carrying rate of food with attractants is more than 90%. The results show that attractant ingredients can enhance the attractant properties of baits to red fire ants. However, only under specific attractant ingredients can the bait achieve the effect. The data of comparative example 1 shows that (E, E)-α-farnesene has no attractant effect, and only the (Z, E)-α-farnesene configuration has an attractant effect.

[0054] Preparation of red fire ant bait:

[0055] Example 1: 0.025% spinosad·0.025% indoxacarb bait

[0056] The red fire ant bait is 0.25g of spinosad, 0.25g of indoxacarb, 0.08g of (Z,E)-α-farnesene, 0.12g of 2-ethyl-3,6-dimethylpyrazine, 30g of benzoic acid, 100g of crushed peanuts, and corn grits to make up to 1000g.

[0057] Example 2: 0.02% spinosad·0.05% indoxacarb bait

[0058] The red fire ant bait is 0.2g of spinosad, 0.5g of indoxacarb, 0.04g of (Z,E)-α-farnesene, 0.06g of 2-ethyl-3,6-dimethylpyrazine, 40g of benzoic acid, 100g of crushed peanuts, 100g of meat powder, 100g of yeast glucan, and corn grits are added to make up to 1000g.

[0059] Example 3: 0.05% spinosad·0.02% indoxacarb bait

[0060] The red fire ant bait is 0.5g of spinosad, 0.2g of indoxacarb, 0.075g of (Z,E)-α-farnesene, 0.075g of 2-ethyl-3,6-dimethylpyrazine, 30g of citric acid, 100g of crushed peanuts, and corn grits to make up to 1000g.

[0061] Example 4: 0.05% spinosad·0.05% indoxacarb bait

[0062] The red fire ant bait is 0.5g of spinosad, 0.5g of indoxacarb, 0.075g of (Z,E)-α-farnesene, 0.075g of 2-ethyl-3,6-dimethylpyrazine, 50g of benzoic acid, 100g of crushed peanuts, 100g of meat powder, and corn grits to make up to 1000g.

[0063] Example 5: 0.1% spinosad·0.1% indoxacarb bait

[0064] The red fire ant bait is 1g of spinosad, 1g of indoxacarb, 0.1g of (Z,E)-α-farnesene, 0.1g of 2-ethyl-3,6-dimethylpyrazine, 50g of benzoic acid, 100g of crushed peanuts, 100g of meat powder, 100g of yeast glucan, and corn grits are added to make up to 1000g.

[0065] Comparative Example 1: 0.05 indoxacarb bait

[0066] The finished product Xing'an produced by Jiangsu Gongcheng is available on the market.

[0067] The technical effects of the present invention are described below in conjunction with specific experiments:

[0068] Verification test 2: Co-toxicity coefficient test

[0069] (1) Test subjects: Red imported fire ants

[0070] (2) Test method: Refer to NY / T1154.7-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Pesticides Part 7: Determination of Combined Effects of Mixtures"

[0071] (3) Data statistics: The test agent was first dissolved in dichloromethane, and then serially diluted with an aqueous solution containing 0.1% Triton-X100 at a ratio of 1:9. 1 mL of the drug solution of each dilution concentration was added to a 150 mL serum bottle, and the serum bottle was rotated continuously to make the drug solution evenly distributed on the inner wall of the bottle. The bottle was placed in a fume hood overnight to allow the organic solvent to evaporate completely, and red fire ants were placed in the concentration range of the drug that caused 100% death and 100% survival. Within this range, a suitable concentration was taken and serially diluted with an aqueous solution containing 0.1% Triton-X100. 1 mL of the drug solution of each dilution concentration was added to a 150 mL serum bottle, and the serum bottle was rotated continuously to make the drug solution evenly distributed on the inner wall of the bottle. The bottle was placed in a fume hood overnight to allow the organic solvent to evaporate completely. Vaseline was then applied to the bottleneck of the serum bottle, and 20 test insects were placed in each bottle. The experiment was repeated 5 times. The corresponding solvent treatment was used as the control group. After 1 hour, all test insects were transferred to clean containers for standard feeding. After 24 hours, the number of deaths was checked and counted. The statistical analysis software SPSS was used for analysis to calculate the LC of each agent. 50 and its 95% confidence limit. The results are shown in the following table:

[0072] Table 2 Co-toxicity coefficient of spinosad and indoxacarb to red imported fire ants It can be seen from Table 2 that when the ratio of spinosad: indoxacarb is 5:1 to 1:5, the co-toxicity coefficient to red fire ants is greater than 120, showing a synergistic effect; when the ratio of spinosad: indoxacarb is 1:2.5, the synergistic effect is obvious.

[0073] Verification test 2: Field efficacy test

[0074] (1) Test subjects: Red imported fire ants

[0075] (2) Test method: Select red fire ant nests of equal size and spread 20g of the sample on the nests.

[0076] (3) Test agent:

[0077] Comparative Example 1: 0.05% indoxacarb bait (commercially available); Examples 1-5.

[0078] (4) Statistics:

[0079] Table 3 Results of efficacy tests of several embodiments against red imported fire ants

[0080] Treatment Group Comprehensive prevention effect Example 1 90％ Example 2 95％ Example 3 95％ Example 4 90％ Example 5 90％ Comparative Example 1 70％

[0081] The test results show that the control efficiency of the optimally formulated compound pesticides against red fire ants is 90% or above, with obvious effects.

[0082] The above examples are only for illustrating the technical concept and technical features of the present invention, and cannot be used to limit the protection scope of the present invention. Any equivalent transformation or modification made according to the essence of the present invention should be included in the protection scope of the present invention.

Claims

1. A composition for preventing and controlling red imported fire ants, characterized in that: The composition comprises indoxacarb and spinosad, and the mass ratio of spinosad to indoxacarb is 5:1 to 1:

5.

2. An application of the composition for controlling red imported fire ants according to claim 1, characterized in that: Application of the composition for preventing and controlling red fire ants in the preparation of red fire ant baits.

3. A red fire ant bait, characterized in that: The red fire ant bait comprises a bait, the active ingredient as claimed in claim 1 and an attractant component, wherein the attractant component is farnesene and / or 2-ethyl-3,6-dimethylpyrazine.

4. The red fire ant bait according to claim 3, characterized in that The red fire ant bait comprises, by weight percentage, 0.05% to 0.2% active ingredient, 0.01% to 0.02% attractant ingredient, and the remainder is bait.

5. The red fire ant bait according to claim 1, characterized in that The attractant component is farnesene and 2-ethyl-3,6-dimethylpyrazine in a mass ratio of 2:1 to 1:

2.

6. The red fire ant bait according to claim 5, characterized in that The farnesene is (Z,E)-α-farnesene.

7. The red fire ant bait according to any one of claims 3 to 6, characterized in that: The red fire ant bait also contains a preservative, wherein the amount of the preservative added accounts for 0.3% to 0.5% of the mass of the red fire ant bait.

8. The red fire ant bait according to claim 7, characterized in that The preservative is benzoic acid and / or citric acid.

9. An application of the red fire ant bait according to claim 1, characterized in that: Application of the red fire ant bait in the field of red fire ant prevention and control.

10. The use of the red fire ant bait according to claim 9, characterized in that: The red fire ant bait is prepared as expanded particles.

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