Bird's nest formula product capable of improving kidney yin deficiency and preparation method of bird's nest formula product

Abstract

The invention relates to a cubilose formula product with a function of improving deficiency of kidney-yin and a preparation method of the cubilose formula product, and the cubilose formula product which is good in flavor and has the function of improving deficiency of kidney-yin is developed on the basis of a'component structure 'theory and a'dialectical health-care' theory by taking cubilose as a core medicine flavor and taking a clearing and tonifying effect of the cubilose as a characteristic advantage. According to the preparation method disclosed by the invention, a low-temperature cyclic extraction technology and a low-high-frequency electromagnetic field cyclic auxiliary wrapping technology are adopted for the medicinal and edible food material extraction liquid, more effective components in the food materials are obtained through efficient extraction, and then the effective components are wrapped through the low-high-frequency electromagnetic field cyclic auxiliary wrapping technology; the effect of the product is prevented from being influenced by decomposition and loss of effective components in the reheating process; meanwhile, the tinting strength of the product is reduced through a double-decoloring technology, and the product is endowed with good sense.

Description

Technical Field

[0001] The present invention relates to the field of food, and in particular to a bird's nest formula product for improving kidney yin deficiency function and a preparation method thereof. Background Art

[0002] The kidney is an important organ in the urinary system, responsible for filtering impurities in the blood, maintaining the balance of body fluids and electrolytes, and finally producing urine to be discharged from the body through the urethra. At present, increased pressure in life and work, staying up late, long-term excessive anxiety, excessive smoking and drinking, etc. can all lead to kidney yin deficiency. Common clinical symptoms include soreness of waist and knees, fatigue, premature ejaculation, low sperm count and infertility. Kidney yin deficiency directly affects people's normal life and has become an important treatment trend at present. Therefore, protecting kidney function and avoiding problems such as kidney yin deficiency have a very important impact on physical and mental health.

[0003] For the problem of kidney yin deficiency, the current treatment methods in traditional Chinese medicine are mainly conditioning, and Western medicine treatment is mainly drug treatment. Due to the uncertainty of drug side effects and their efficacy, the general acceptance and clinical promotion are not high. Bird's nest is a traditional precious Chinese medicine that can be used as both medicine and food. Traditional Chinese medicine theory believes that it has the effect of clearing and replenishing, and is a holy medicine for regulating deficiency and fatigue. At present, bird's nest products are single and have single functions, which limits the expansion of bird's nest product categories. Therefore, with bird's nest as the core medicinal flavor, clearing and replenishing effects as the feature, supplemented by the combination of medicinal and edible ingredients, a bird's nest product with the function of improving kidney yin deficiency is studied, which is especially suitable for male consumer groups. It not only achieves the effect of daily nutrition and nourishment, but also can nourish, harmonize, and improve kidney yin deficiency. It is of great significance to the development of bird's nest application products and the male consumer market. Summary of the invention

[0004] The present invention aims to solve one of the technical problems in the related art at least to a certain extent. To this end, one purpose of the present invention is to provide a bird's nest formula product and a preparation method thereof, with bird's nest as the core medicinal flavor and its clearing and nourishing effect as the characteristic advantage, and on the basis of the "component structure" theory and the "dialectical health care" theory, develop a bird's nest formula product for men with good flavor and the effect of improving kidney yin deficiency. The extract of the food and medicine ingredients in the preparation method of the present invention adopts low-temperature circulation extraction technology and low-high frequency electromagnetic field circulation assisted packaging technology, wherein more effective ingredients in the ingredients are efficiently extracted, and then the effective ingredients are packaged by low-high frequency electromagnetic field circulation assisted packaging technology to avoid the decomposition and loss of effective ingredients during the reheating process and affect the efficacy of the product; at the same time, the coloring power of the product is reduced by double decolorization technology, giving the product a good sensory feel.

[0005] To this end, the first aspect of the present invention provides a composition. In some embodiments of the present invention, the composition comprises:

[0006] A first material, wherein the raw material of the first material includes bird's nest;

[0007] The second material comprises the following raw materials: ginseng, Cistanche deserticola, Dendrobium candidum, fresh Imperata root, Poria cocos, Eucommia ulmoides male flower, crystal sugar and Cordyceps militaris.

[0008] The present invention is based on the theory of traditional Chinese medicine, takes bird's nest as the core medicinal flavor, and takes its clearing and nourishing effects as its characteristic advantage. On the basis of the "component structure" theory and the "dialectical health care" theory, data mining is used to develop ingredients of bird's nest products suitable for men. Cistanche, Eucommia ulmoides and Cordyceps militaris are used as monarch medicines, ginseng and Dendrobium officinale are used as assistant medicines, Poria is used as a messenger medicine, and fresh Imperata root and bird's nest are used as auxiliary medicines. It has the function of regulating body disorders and improving kidney yin deficiency, and develops a bird's nest product suitable for men to eat.

[0009] In some embodiments of the present invention, the raw material of the second material further includes cyclodextrin.

[0010] In some embodiments of the present invention, the mass ratio of the first material to the second material is (5-15):(40-140).

[0011] In some embodiments of the present invention, in the second material, the mass ratio of ginseng, Cistanche deserticola, Dendrobium officinale, fresh Imperata root, Poria cocos, Eucommia ulmoides male flower, crystal sugar, Cordyceps militaris, and cyclodextrin is (1-3): (5-15): (5-20), (5-45): (5-15): (5-10): (5-15): (5-15): (0.5-1.0).

[0012] In some embodiments of the present invention, the first material is a liquid or a dry solid.

[0013] In some embodiments of the present invention, the second material is a liquid.

[0014] In some embodiments of the present invention, the first material and the second material are placed separately or mixed.

[0015] The second aspect of the present invention provides use of the composition described in the first aspect in preparing a bird's nest formula product for improving kidney yin deficiency function.

[0016] The third aspect of the present invention provides a bird's nest formula product. In some embodiments of the present invention, the bird's nest formula product comprises the composition described in the first aspect.

[0017] The present invention provides a bird's nest product with the function of improving kidney yin deficiency. The product has the functions of regulating body disorders and improving kidney yin deficiency through animal experimental function evaluation, and the unique advantages of the product are suitable for male consumer groups.

[0018] The fourth aspect of the present invention provides a method for preparing the bird's nest formula product described in the second aspect. In some embodiments of the present invention, the preparation method comprises:

[0019] S1: pre-treating the bird's nest raw material to obtain the first material;

[0020] S2: Mix the raw materials of ginseng, Cistanche deserticola, Dendrobium officinale, fresh Imperata root, Poria cocos, Eucommia ulmoides male flower, and Cordyceps militaris, and use low-temperature circulation extraction technology to obtain the medicinal and edible extract R1;

[0021] S3: performing double decolorization treatment on the food-medicine-susceptible extract R1 to obtain the food-medicine-susceptible extract R2;

[0022] S4: adding cyclodextrin to the food-medicine homologous extract R2, and using low-high frequency electromagnetic field circulation-assisted encapsulation technology to encapsulate the active ingredients in the food-medicine homologous mixed solution R2, so as to obtain the food-medicine homologous extract R3;

[0023] S5: Mix the first material with the food and medicine extract R3 to obtain the bird's nest formula product.

[0024] At present, the bird's nest products on the market are mainly for female tonic, and there is a lack of bird's nest products for men; and most bird's nest products are mainly pure bird's nest with an appropriate amount of sugar added, and there are few products that combine bird's nest with medicine and food based on function orientation; the protein in the bird's nest will undergo a corresponding Maillard reaction with the polysaccharides in the medicine and food ingredients, and during the heating process, the interaction between the Maillard and caramelization products will produce an oxidation reaction to generate large molecules, which improves the coloring power of the product, and excessive reaction will generate black-brown high-molecular compounds, which makes the product have poor sensory perception; the extraction of ingredients in medicine and food ingredients is mainly based on extraction techniques such as water extraction and alcohol precipitation, countercurrent extraction, and ultrasonic extraction, but they have the problems of slow efficiency and few extracted ingredients; at the same time, during the heating process of medicine and food ingredients, some effective ingredients react, decompose, and are lost, resulting in limited product functional effects.

[0025] The present invention solves the problems of dark color, low extraction efficiency and few ingredients of the product of bird's nest combined with food and medicine by screening and studying the preparation process of food and medicine ingredients that can be combined with bird's nest; the treatment of kidney yin deficiency is currently mainly based on the combination of Chinese and Western medicine, but the slow effect of conventional food supplements and the bitter and unpalatable characteristics of Chinese medicine limit the population with certain needs. This application is guided by the target function, based on the theory of traditional Chinese medicine, with bird's nest as the core medicinal flavor, and its clearing and nourishing effect as its characteristic advantage. On the basis of the theory of "component structure" and "dialectical health care", a male bird's nest formula product with good flavor and the effect of improving kidney yin deficiency is developed through data mining.

[0026] The preparation method provided by the present invention adopts low-temperature circulation extraction technology and low-high frequency electromagnetic field circulation assisted packaging technology to efficiently extract more effective ingredients in the food, and then the effective ingredients are packaged by low-high frequency electromagnetic field circulation assisted packaging technology to avoid decomposition and loss of effective ingredients during reheating, thereby affecting the efficacy of the product; at the same time, the coloring power of the product is reduced by double decolorization technology to give the product a good sensory experience.

[0027] In some embodiments of the present invention, the pre-treatment comprises:

[0028] The bird's nest raw materials are removed from the raw materials and dried.

[0029] In some embodiments of the present invention, in step S2, the extraction temperature using low-temperature cycle extraction technology is 50-70°C, and the extraction time is 40-50 minutes.

[0030] Low-temperature extraction can achieve selective extraction of specific components based on the differences in solubility and vapor pressure of the substance at different temperatures. The preparation method of the bird's nest formula product provided by the present invention adopts specific low-temperature cycle extraction conditions, which can be extracted under low-temperature conditions, further reducing the oxidative decomposition loss of heat-sensitive active ingredients, which is beneficial to protecting the activity of natural products.

[0031] In some embodiments of the present invention, in step S3, the double decolorization treatment includes decolorizing the edible and medicinal extract R1 using activated carbon and water membrane.

[0032] Activated carbon is a highly porous carbon material with a huge specific surface area. Its pore structure can provide a large number of adsorption sites, making it have a strong adsorption capacity. When activated carbon is added in the process of the present invention, the activated carbon will contact with the pigment molecules in the liquid, and the pigment molecules will be adsorbed to the surface or pores of the activated carbon, thereby achieving a decolorization effect.

[0033] In some embodiments of the present invention, in step S4, the mass ratio of the amount of cyclodextrin added to the total weight of the raw materials mixed in step S1 is (0.5-1.0): (31-123).

[0034] Cyclodextrin is a product generated by acid hydrolysis and cyclization of starch. It can encapsulate various compound molecules, increase the stability of the encapsulated substances to light, heat and oxygen, and change the physical and chemical properties of the encapsulated substances. In step S4, cyclodextrin is added to the food-medicine homologous extract, and the active ingredients of the food-medicine homologous mixture are encapsulated by low-frequency electromagnetic field circulation-assisted encapsulation technology. The oxidative decomposition loss of heat-sensitive active ingredients can be further reduced by low-temperature electromagnetic field heating.

[0035] In some embodiments of the present invention, the parameters of the low-high frequency electromagnetic field cycle-assisted packaging technology are: magnetic field intensity 200-300 mT, time 10-20 min.

[0036] In some embodiments of the present invention, the preparation method further comprises: before performing step S5, adding crystal sugar to the medicine-food homologous extract R3 obtained in step S4.

[0037] In some embodiments of the present invention, in step S5, when the first material is solid, when the first material is mixed with the food and medicine extract R3, the mass volume ratio is (5-15): (100-200) g / ml.

[0038] In some embodiments of the present invention, step S5 further includes sterilizing the mixture of the first material and the food and medicine extract R3.

[0039] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0041] Figure 1 The color diagram of the product prepared by the process of Example 1 (control sample) and Comparative Example 2 is shown;

[0042] Figure 2a-2c The effects on serum cAMP, cGMP levels, and cAMP / cGMP ratio of mice in different groups were shown;

[0043] Figure 3 The effects of the bird's nest formula product on the general morphology of mice with kidney yin deficiency were shown;

[0044] Figure 4 The effect of bird's nest formula products on the body weight, body temperature, food intake and water intake of mice with kidney yin deficiency is shown; (A) The trend of weight changes of mice in different groups; (B) The change of body temperature of mice in different groups; (C) The trend of food intake changes of mice in different groups; (D) The trend of water intake changes of mice in different groups (n=10);

[0045] Figure 5 Figure AC shows the effect of men's bird's nest products on serum cAMP and cGMP levels in mice with kidney yin deficiency (n=6), vs. control group. ## P<0.01, ### P<0.001; vs. model group, ## P<0.01,### P < 0.001;

[0046] Figure 6 Figure AC shows the effect of bird's nest formula products on reproductive hormones T and E2 in mice with kidney yin deficiency (n=6), vs. control group. ## P<0.01, ### P<0.001; vs. model group, ## P<0.01, ### P < 0.001;

[0047] Figure 7 The effect of bird's nest formula products on kidney tissue of mice with kidney yin deficiency was shown;

[0048] Figure 8 The effect of bird's nest formula products on semen quality of mice with kidney yin deficiency was shown;

[0049] Fig. 9 The results show the effect of bird's nest formula product on the testicular tissue morphology of mice with kidney yin deficiency. DETAILED DESCRIPTION

[0050] The embodiments of the present invention are described in detail below. The embodiments described below are exemplary and are only used to explain the present invention, and should not be understood as limiting the present invention.

[0051] It should be noted that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. Further, in the description of the present invention, unless otherwise specified, the meaning of "plurality" is two or more.

[0052] The endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0053] In order to make the present invention more easily understood, certain technical and scientific terms are specifically defined below. Unless otherwise clearly defined elsewhere in this document, all other technical and scientific terms used herein have the meanings commonly understood by those skilled in the art to which the present invention belongs.

[0054] In this document, the terms “include” or “comprising” are open expressions, that is, including the contents specified in the present invention but not excluding other contents.

[0055] As used herein, the terms "optionally", "optional" or "optionally" generally mean that the subsequently described event or circumstance may but need not occur, and that the description includes instances where the event or circumstance occurs and instances where it does not.

[0056] In this article, "low-temperature circulation extraction technology" refers to the technology of using a solvent (such as pure water) to extract one substance (active ingredient) from another substance (referring to medicinal materials in this article) in a low-temperature circulation manner in order to obtain more active ingredients.

[0057] In this article, "low-high frequency electromagnetic field cycle-assisted encapsulation technology" refers to placing the encapsulation agent (such as cyclodextrin in this article) into a low-high frequency electromagnetic device for cyclic heat conduction heating to better encapsulate the active ingredients in order to obtain more active ingredients.

[0058] In this article, "double decolorization technology" refers to the technology of using two or more decolorization methods in combination to achieve better decolorization effect. This article specifically refers to the technology of double decolorization using activated carbon and filter membrane, so as to effectively decolorize the bird's nest products and give the products better sensory effects.

[0059] The present invention relates to a bird's nest product formula with the function of improving kidney yin deficiency and a preparation method thereof. Traditional Chinese medicine believes that kidney yin deficiency refers to deficiency of kidney yin and internal disturbance of virtual heat, which is normally caused by long-term illness and kidney damage, or excessive sexual life. Clinically, it manifests as: dizziness, tinnitus and deafness, insomnia and dreaminess, hot flashes and night sweats, soreness of waist and knees, red tongue and dry fur, male spermatorrhea, etc. The present invention takes "benefiting the source of fire to eliminate yin" as the theoretical basis, takes tonifying the kidney and assisting yang as the method, and supplements with diuresis and moisture elimination to achieve the effect of improving kidney yin deficiency.

[0060] According to a specific embodiment of the present invention, the present invention provides a bird's nest formula product, comprising:

[0061] A first material, wherein the raw material of the first material includes bird's nest;

[0062] The second material comprises the following raw materials: ginseng, Cistanche deserticola, Dendrobium candidum, fresh Imperata root, Poria cocos, Eucommia ulmoides male flower, crystal sugar and Cordyceps militaris.

[0063] According to a specific embodiment of the present invention, the raw material of the second material further includes cyclodextrin.

[0064] According to a specific embodiment of the present invention, the mass ratio of the first material to the second material is (5-15): (40-140).

[0065] According to a specific embodiment of the present invention, in the second material, the mass ratio of ginseng, Cistanche deserticola, Dendrobium officinale, fresh Imperata root, Poria cocos, Eucommia ulmoides male flower, crystal sugar, Cordyceps militaris and cyclodextrin is (1-3): (5-15): (5-20), (5-45): (5-15): (5-10): (5-15): (5-15): (0.5-1.0).

[0066] According to a specific embodiment of the present invention, the first material is liquid or dry solid.

[0067] According to a specific embodiment of the present invention, the second material is liquid.

[0068] According to a specific embodiment of the present invention, the first material and the second material are placed separately or mixed.

[0069] According to a specific embodiment of the present invention, the present invention provides a male bird's nest product with the function of improving kidney yin deficiency, comprising the following raw materials in parts by weight:

[0070] 5-15 grams of bird's nest, 1-3 grams of ginseng, 5-15 grams of Cistanche deserticola, 5-20 grams of Dendrobium officinale, 5-15 grams of Poria cocos, 5-45 grams of fresh Imperata root, 5-10 grams of Eucommia ulmoides flower, 5-15 grams of Cordyceps militaris, and 5-15 grams of crystal sugar.

[0071] The above-mentioned formula of the men's bird's nest product, the preferred medicinal and edible ingredients can be coordinated with the bird's nest to enhance immunity and improve kidney yin deficiency; secondly, the ingredient extract has no obvious sourness and astringency, and the characteristic flavor is better.

[0072] The formula uses bird's nest, Cordyceps militaris, Dendrobium candidum, and ginseng to nourish kidney yin, Cistanche deserticola and Eucommia ulmoides male flowers to warm the kidney and boost yang, and the yin and yang complement each other, and the hardness and softness complement each other, so that the kidney's vital energy is infinite; Poria cocos is used to promote water and eliminate dampness, and fresh Imperata root is used to regulate blood stagnation. The combination of these medicines can help the weak yang to transform water, and nourish the weak yin to invigorate vitality. It reflects the advantages of TCM's overall regulation, and produces the effects of nourishing, calming, moisturizing, and nourishing the kidney yin.

[0073] According to a specific embodiment of the present invention, the present invention provides a method for preparing a bird's nest formula product, comprising:

[0074] S1: pre-treating the bird's nest raw material to obtain the first material;

[0075] S2: Mix the raw materials of ginseng, Cistanche deserticola, Dendrobium officinale, fresh Imperata root, Poria cocos, Eucommia ulmoides male flower, and Cordyceps militaris, and use low-temperature circulation extraction technology to obtain the medicinal and edible extract R1;

[0076] S3: performing double decolorization treatment on the food-medicine-susceptible extract R1 to obtain the food-medicine-susceptible extract R2;

[0077] S4: adding cyclodextrin to the food-medicine homologous extract R2, and using low-high frequency electromagnetic field circulation-assisted encapsulation technology to encapsulate the active ingredients in the food-medicine homologous mixed solution R2, so as to obtain the food-medicine homologous extract R3;

[0078] S5: Mix the first material with the food and medicine extract R3 to obtain the bird's nest formula product.

[0079] According to a specific embodiment of the present invention, the pre-treatment comprises:

[0080] The bird's nest raw materials are removed from the raw materials and dried.

[0081] According to a specific embodiment of the present invention, in step S2, the extraction temperature using the low-temperature cycle extraction technology is 50-70°C, and the extraction time is 40-50 minutes.

[0082] Because edible and medicinal ingredients contain effective ingredients such as polyphenols, flavonoids, and polysaccharides, low temperature can reduce the loss of effective ingredients, and cyclic extraction can increase the content of effective ingredients.

[0083] It should be noted that in the preparation method of the bird's nest formula product provided by the present invention, there is no particular restriction on the combination of the two decolorization methods used in the double decolorization treatment. According to a preferred embodiment of the present invention, in step S3, the double decolorization treatment includes decolorizing the medicinal and edible extract R1 using activated carbon and water film.

[0084] There is no particular limitation on whether the double decolorization treatment is to first use activated carbon and then use water membrane adsorption, or first use water membrane and then use activated carbon. Preferably, activated carbon adsorption is first used and then water membrane filtration is used.

[0085] It should be noted that there is no particular restriction on the weight-volume ratio of activated carbon to the edible and medicinal extract R1 to be decolorized. The amount of activated carbon added is sufficient to prevent the color of the edible and medicinal extract R1 from changing. For example, the weight-volume ratio of activated carbon to the edible and medicinal extract R1 to be decolorized is 0.1 g / ml. There is no particular restriction on the particle size of the water film. As long as the color of the edible and medicinal extract R1 after adsorption by activated carbon becomes lighter, such water films are within the scope of protection of the present invention. Preferably, a 0.22 μm water film is used to further filter the edible and medicinal extract R1 after adsorption by activated carbon.

[0086] Because food and medicine ingredients are dark in color, a specific double decolorization method can be used to reduce the coloring power of the product and give the product a good sensory experience.

[0087] According to a specific embodiment of the present invention, in step S4, the mass ratio of the amount of cyclodextrin added to the total weight of the raw materials mixed in step S1 is (0.5-1.0): (31-123).

[0088] According to a specific embodiment of the present invention, the parameters of the low-high frequency electromagnetic field cycle-assisted packaging technology are: magnetic field intensity 200-300 mT, time 10-20 min.

[0089] Because the men's bird's nest product will eventually be sterilized at high temperature to ensure commercial sterility, the active ingredients in the extract R2 obtained at that time will be at risk of secondary decomposition and loss, affecting its efficacy. Therefore, its components are encapsulated to effectively avoid the loss of active ingredients in the extract during high-temperature sterilization.

[0090] According to a specific embodiment of the present invention, in step S5, when the first material is solid, when the first material is mixed with the medicinal and edible extract R3, the mass volume ratio is (5-15): (100-200) g / ml.

[0091] According to a specific embodiment of the present invention, step S5 further includes sterilizing the mixture of the first material and the food and medicine extract R3.

[0092] According to a specific embodiment of the present invention, the technical solution of a preparation method of a bird's nest formula product having the function of improving kidney yin deficiency provided by the present invention is as follows:

[0093] The above-mentioned bird's nest formula product comprises two parts: bird's nest (A) and medicine and food extract (B);

[0094] The above-mentioned bird's nest (A) includes the following raw materials by weight: 5-15 grams of bird's nest, and the corresponding preparation method is as follows:

[0095] The pure bird's nest is soaked for 1-2 hours, picked to remove impurities such as black spots, bird feathers, and eggshells, then washed, spun, and dried to obtain the bird's nest (A);

[0096] The above-mentioned bird's nest formula product medicinal and edible extract (B) includes the following raw materials by weight: 1-3 grams of ginseng, 5-15 grams of Cistanche deserticola, 5-20 grams of Dendrobium candidum, 5-15 grams of Poria cocos, 5-45 grams of fresh Imperata root, 5-10 grams of Eucommia male flower, 5-15 grams of Cordyceps militaris, and 5-15 grams of crystal sugar. The corresponding preparation method is as follows:

[0097] (1) Mix the above weights of Cordyceps militaris, ginseng, Cistanche deserticola, Dendrobium officinale, Poria cocos, fresh Imperata cylindrica, and Eucommia ulmoides male flowers, add 30-50 times of purified water, and stir and mix for 5-10 minutes;

[0098] (2) Using low-temperature circulation extraction technology, wait for the circulation extraction tank to heat up to 70°C and start extraction for 40-50 minutes;

[0099] (3) After the extraction is completed, the extract R1 is obtained by filtering through 300 mesh;

[0100] (4) Using double decolorization technology, activated carbon (0.1-0.3) w / v was added to the extract R1, stirred and dissolved, and then passed through a 0.22 μm water film to obtain a prepared extract R2;

[0101] (5) using low-high frequency electromagnetic field cycle-assisted encapsulation technology, adding 0.5-1.0 g of cyclodextrin to the extract R2 to encapsulate the active ingredients, and placing it in a low-high frequency electromagnetic heating device for cycle heat conduction heating for 10-20 min to obtain extract R3;

[0102] (6) Add 5-15 g of crystal sugar into the extract R3, heat to 90° C. to completely dissolve, and weigh to obtain a medicinal and edible extract (B);

[0103] (7) placing the bird's nest (A) and the edible and medicinal extract (B) into a glass bottle according to certain specifications and sealing the bottle;

[0104] (8) Sterilization, 110-120°C, 15-20 min;

[0105] (9) Finished product.

[0106] The bird's nest formula product with the function of improving kidney yin deficiency and the preparation method thereof of the present invention have the characteristics of attractive color, outstanding medicinal and edible flavor, smooth and full taste, slightly sweet and non-astringent. The product is ready to eat after opening, providing more convenient service for white-collar workers in the new era, and through animal test, the bird's nest formula product has the effect of alleviating symptoms and regulating body disorders in kidney yin deficiency model mice.

[0107] The present invention develops a bird's nest formula product that has the effect of improving kidney yin deficiency, takes bird's nest as the core medicinal flavor, has the comprehensive characteristic flavor of medicine and food, mellow taste, smooth taste, and smooth and full taste of bird's nest through the screening of bird's nest and medicinal and edible ingredients. Through the innovation of decolorization process, the problem of dark color and poor sensory perception of medicinal and edible ingredients is solved; through the innovation of extraction process, the decomposition and loss of effective ingredients of medicinal and edible ingredients are avoided; through the innovation of packaging technology and process, the loss of effective ingredients in high-temperature sterilization process is avoided. The product is ready to eat after opening, providing more convenient service for white-collar workers in the new era. According to animal experiments, the bird's nest formula product has the effects of alleviating symptoms and regulating body disorders on mice with kidney yin deficiency, especially has a good improvement effect on sperm and testicles of men, and is particularly suitable for male consumer groups.

[0108] The scheme of the present disclosure will be explained below in conjunction with the examples. Those skilled in the art will appreciate that the following examples are only used to illustrate the present disclosure and should not be considered to limit the scope of the present disclosure. Where specific techniques or conditions are not indicated in the examples, the techniques or conditions described in the literature in this area or the product instructions are used. Where the manufacturers of reagents or instruments are not indicated, they are all conventional products that can be obtained commercially.

[0109] Example 1 Preparation of bird's nest formula product

[0110] The bird's nest formula product of this embodiment includes two parts: bird's nest (A) and medicine and food extract (B).

[0111] Bird's nest (A) includes the following raw materials in parts by weight: 5 g of bird's nest, and the corresponding preparation method is as follows:

[0112] The pure bird's nest was soaked for 2 hours, and then black spots, bird's hair, eggshell and other impurities were removed by picking, washing, drying and drying to obtain the bird's nest (A).

[0113] The edible and medicinal extract (B) comprises the following raw materials in parts by weight: 1 gram of ginseng, 5 grams of Cistanche deserticola, 5 grams of Dendrobium officinale, 5 grams of fresh Imperata root, 5 grams of Poria cocos, 5 grams of Eucommia male flower, 5 grams of Cordyceps militaris, and 5 grams of crystal sugar.

[0114] The preparation method of the bird's nest formula product is as follows:

[0115] (1) Mix the above Cordyceps militaris, ginseng, Cistanche deserticola, Dendrobium officinale, Poria cocos, Eucommia ulmoides male flower, and fresh Imperata cylindrica root, add 50 times of purified water, and stir and mix for 10 minutes;

[0116] (2) Using low-temperature circulation extraction technology, wait for the circulation extraction tank to be heated to 70°C and start extraction for 40 minutes;

[0117] (3) After the extraction is completed, the extract R1 is obtained by filtering through 300 mesh;

[0118] (4) Using double decolorization technology, 0.1 w / v of activated carbon was added to the extract R1, stirred and dissolved, and then passed through a 0.22 μm water film to obtain a prepared extract R2;

[0119] (5) using low-high frequency electromagnetic field cycle-assisted encapsulation technology, adding 0.5 g of cyclodextrin to the extract R2 to encapsulate the active ingredients, and placing it in a low-high frequency electromagnetic heating device for cycle heat conduction heating at a magnetic field intensity of 220 mT for 10 min to obtain extract R3;

[0120] (6) Add 5 g of crystal sugar into the extract R3, heat to 90° C. to completely dissolve, and weigh to obtain a medicinal and edible extract (B);

[0121] (7) placing the bird's nest (A) and the edible and medicinal extract (B) into a glass bottle according to certain specifications and sealing the bottle;

[0122] (8) Sterilization, 110°C, 20 min;

[0123] (9) Finished product.

[0124] Example 2 Preparation of bird's nest formula product

[0125] The bird's nest formula product of this embodiment includes two parts: bird's nest (A) and medicine and food extract (B).

[0126] Bird's nest (A) includes the following raw materials in parts by weight: 15 g of bird's nest, and the corresponding preparation method is as follows:

[0127] The pure bird's nest was soaked for 2 hours, sorted to remove impurities such as black spots, bird feathers, and eggshells, and then washed, spun, and dried to obtain the bird's nest (A).

[0128] The edible and medicinal extract (B) comprises the following raw materials in parts by weight: 3 grams of ginseng, 15 grams of Cistanche deserticola, 20 grams of Dendrobium candidum, 45 grams of fresh Imperata root, 15 grams of Poria cocos, 10 grams of Eucommia male flower, 15 grams of Cordyceps militaris, and 15 grams of crystal sugar.

[0129] The preparation method of the bird's nest formula product is as follows:

[0130] (1) Mix the above Cordyceps militaris, ginseng, Cistanche deserticola, Dendrobium officinale, Poria cocos, Eucommia ulmoides male flower, and fresh Imperata cylindrica root, add 50 times of purified water, and stir and mix for 10 minutes;

[0131] (2) Using low-temperature circulation extraction technology, wait for the circulation extraction tank to be heated to 70°C and start extraction for 40 minutes;

[0132] (3) After the extraction is completed, the extract R1 is obtained by filtering through 300 mesh;

[0133] (4) Using double decolorization technology, 0.1 w / v of activated carbon was added to the extract R1, stirred and dissolved, and then passed through a 0.22 μm water film to obtain a prepared extract R2;

[0134] (5) using low-high frequency electromagnetic field cycle-assisted encapsulation technology, adding 0.5 g of cyclodextrin to the extract R2 to encapsulate the active ingredients, and placing it in a low-high frequency electromagnetic heating device for cycle heat conduction heating at a magnetic field intensity of 240 mT for 10 min to obtain extract R3;

[0135] (6) Add 15 g of crystal sugar into the extract R3, heat to 90° C. to completely dissolve, and weigh to obtain a medicinal and edible extract (B);

[0136] (7) placing the bird's nest (A) and the edible and medicinal extract (B) into a glass bottle according to certain specifications and sealing the bottle;

[0137] (8) Sterilization, 110°C, 20 min;

[0138] (9) Finished product.

[0139] Example 3 Preparation of bird's nest formula product

[0140] The bird's nest formula product of this embodiment includes two parts: bird's nest (A) and medicine and food extract (B).

[0141] Bird's nest (A) includes the following raw materials in parts by weight: 5 g of bird's nest, and the corresponding preparation method is as follows:

[0142] The pure bird's nest was soaked for 2 hours, and then black spots, bird's hair, eggshell and other impurities were removed by picking, washing, drying and drying to obtain the bird's nest (A).

[0143] The edible and medicinal extract (B) comprises the following raw materials in parts by weight: 1 gram of ginseng, 5 grams of Cistanche deserticola, 5 grams of Dendrobium officinale, 5 grams of fresh Imperata root, 5 grams of Poria cocos, 5 grams of Eucommia male flower, 5 grams of Cordyceps militaris, and 5 grams of crystal sugar.

[0144] The preparation method of the bird's nest formula product is as follows:

[0145] (1) Mix the above Cordyceps militaris, ginseng, Cistanche deserticola, Dendrobium officinale, Poria cocos, Eucommia ulmoides male flower, and fresh Imperata cylindrica root, add 50 times of purified water, and stir and mix for 10 minutes;

[0146] (2) Using low-temperature circulation extraction technology, wait for the circulation extraction tank to be heated to 70°C and start extraction for 50 minutes;

[0147] (3) After the extraction is completed, the extract R1 is obtained by filtering through 300 mesh;

[0148] (4) Using double decolorization technology, 0.1 w / v of activated carbon was added to the extract R1, stirred and dissolved, and then passed through a 0.22 μm water film to obtain a prepared extract R2;

[0149] (5) using low-high frequency electromagnetic field cycle-assisted encapsulation technology, adding 0.5 g of cyclodextrin to the extract R2 to encapsulate the active ingredients, and placing it in a low-high frequency electromagnetic heating device for cycle heat conduction heating at a magnetic field intensity of 220 mT for 20 min to obtain extract R3;

[0150] (6) Add 5 g of crystal sugar into the extract R3, heat to 90° C. to completely dissolve, and weigh to obtain a medicinal and edible extract (B);

[0151] (7) placing the bird's nest (A) and the edible and medicinal extract (B) into a glass bottle according to certain specifications and sealing the bottle;

[0152] (8) Sterilization, 110°C, 20 min;

[0153] (9) Finished product.

[0154] Example 4 Preparation of bird's nest formula product

[0155] The bird's nest formula product of this embodiment includes two parts: bird's nest (A) and medicine and food extract (B).

[0156] Bird's nest (A) includes the following raw materials in parts by weight: 5 g of bird's nest, and the corresponding preparation method is as follows:

[0157] The pure bird's nest was soaked for 2 hours, and then black spots, bird's hair, eggshells and other impurities were removed by picking, washing, drying and drying to obtain the bird's nest (A).

[0158] The edible and medicinal extract (B) comprises the following raw materials in parts by weight: 1 gram of ginseng, 5 grams of Cistanche deserticola, 5 grams of Dendrobium officinale, 5 grams of fresh Imperata root, 5 grams of Poria cocos, 5 grams of Eucommia male flower, 5 grams of Cordyceps militaris, and 5 grams of crystal sugar.

[0159] The preparation method of the bird's nest formula product is as follows:

[0160] (1) Mix the above Cordyceps militaris, ginseng, Cistanche deserticola, Dendrobium officinale, Poria cocos, Eucommia ulmoides male flower, and fresh Imperata cylindrica root, add 50 times of purified water, and stir and mix for 10 minutes;

[0161] (2) Using low-temperature circulation extraction technology, wait for the circulation extraction tank to be heated to 70°C and start extraction for 40 minutes;

[0162] (3) After the extraction is completed, the extract R1 is obtained by filtering through 300 mesh;

[0163] (4) Using double decolorization technology, 0.3 w / v of activated carbon was added to the extract R1, stirred and dissolved, and then passed through a 0.22 μm water film to obtain a prepared extract R2;

[0164] (5) using low-high frequency electromagnetic field cycle-assisted encapsulation technology, adding 0.5 g of cyclodextrin to the extract R2 to encapsulate the active ingredients, and placing it in a low-high frequency electromagnetic heating device for cycle heat conduction heating at a magnetic field intensity of 220 mT for 10 min to obtain extract R3;

[0165] (6) Add 5 g of crystal sugar into the extract R3, heat to 90° C. to completely dissolve, and weigh to obtain a medicinal and edible extract (B);

[0166] (7) placing the bird's nest (A) and the edible and medicinal extract (B) into a glass bottle according to certain specifications and sealing the bottle;

[0167] (8) Sterilization, 110°C, 20 min;

[0168] (9) Finished product.

[0169] It has been determined that the bird's nest formula products of Examples 1-4 have good appearance and color, have the comprehensive flavor characteristics of medicinal and edible ingredients, have a mellow and smooth taste, and the bird's nest has a smooth and full taste.

[0170] Comparative Example 1

[0171] The bird's nest formula product of this embodiment includes two parts: bird's nest (A) and medicine and food extract (B).

[0172] Bird's nest (A) includes the following raw materials in parts by weight: 5 g of bird's nest, and the corresponding preparation method is as follows:

[0173] The pure bird's nest was soaked for 2 hours, and then black spots, bird's hair, eggshell and other impurities were removed by picking, washing, drying and drying to obtain the bird's nest (A).

[0174] The edible and medicinal extract (B) comprises the following raw materials in parts by weight: 1 gram of ginseng, 5 grams of Cistanche deserticola, 5 grams of Dendrobium officinale, 5 grams of fresh Imperata root, 5 grams of Poria cocos, 5 grams of Eucommia male flower, 5 grams of Cordyceps militaris, and 5 grams of crystal sugar.

[0175] The preparation method of the bird's nest formula product is as follows:

[0176] (1) Mix the above Cordyceps militaris, ginseng, Cistanche deserticola, Dendrobium officinale, Poria cocos, Eucommia ulmoides male flower, and fresh Imperata cylindrica root, add 50 times of purified water, and stir and mix for 10 minutes;

[0177] (2) The extraction tank was heated to 95°C and extraction started for 30 minutes;

[0178] (3) After the extraction is completed, the extract R1 is obtained by filtering through 300 mesh;

[0179] (4) Using double decolorization technology, 0.1 w / v of activated carbon was added to the extract R1, stirred and dissolved, and then passed through a 0.22 μm water film to obtain a prepared extract R2;

[0180] (5) using low-high frequency electromagnetic field cycle-assisted encapsulation technology, adding 0.5 g of cyclodextrin to the extract R2 to encapsulate the active ingredients, and placing it in a low-high frequency electromagnetic heating device for cycle heat conduction heating at a magnetic field intensity of 220 mT for 10 min to obtain extract R3;

[0181] (6) Add 5 g of crystal sugar into the extract R3, heat to 90° C. to completely dissolve, and weigh to obtain a medicinal and edible extract (B);

[0182] (7) placing the bird's nest (A) and the edible and medicinal extract (B) into a glass bottle according to certain specifications and sealing the bottle;

[0183] (8) Sterilization, 110°C, 20 min;

[0184] (9) Finished product.

[0185] Effect of extraction process on active ingredients

[0186] The product of Comparative Example 1 was taken as a control group with Example 1 to test the effective ingredients of the finished product. Five groups were tested in parallel and the average value was taken. The results are shown in Table 1.

[0187] Table 1 Effect of extraction process on the content of active ingredients

[0188]

[0189] As can be seen from Table 1, the content of saponin, adenosine and polysaccharide in the product provided by Example 1 is significantly higher than that in Comparative Example 1, indicating that the use of specific low-temperature cyclic extraction technology and extraction conditions can further improve the extraction of effective ingredients; indicating that under this extraction technology, low temperature can reduce the loss of effective ingredients, and cyclic extraction can increase the content of effective ingredients.

[0190] Comparative Example 2

[0191] The bird's nest formula product of this embodiment includes two parts: bird's nest (A) and medicine and food extract (B).

[0192] Bird's nest (A) includes the following raw materials in parts by weight: 5 g of bird's nest, and the corresponding preparation method is as follows:

[0193] The pure bird's nest was soaked for 2 hours, and then black spots, bird's hair, eggshell and other impurities were removed by picking, washing, drying and drying to obtain the bird's nest (A).

[0194] The edible and medicinal extract (B) comprises the following raw materials in parts by weight: 1 gram of ginseng, 5 grams of Cistanche deserticola, 5 grams of Dendrobium officinale, 5 grams of fresh Imperata root, 5 grams of Poria cocos, 5 grams of Eucommia male flower, 5 grams of Cordyceps militaris, and 5 grams of crystal sugar.

[0195] The preparation method of the bird's nest formula product is as follows:

[0196] (1) Mix the above Cordyceps militaris, ginseng, Cistanche deserticola, Dendrobium officinale, Poria cocos, Eucommia ulmoides male flower, and fresh Imperata cylindrica root, add 50 times of purified water, and stir and mix for 10 minutes;

[0197] (2) Using low-temperature circulation extraction technology, wait for the circulation extraction tank to be heated to 70°C and start extraction for 40 minutes;

[0198] (3) After the extraction is completed, the extract R1 is obtained by filtering through 300 mesh;

[0199] (4) using low-high frequency electromagnetic field cycle-assisted encapsulation technology, adding 0.5 g of cyclodextrin to the extract R1 to encapsulate the active ingredients, and placing it in a low-high frequency electromagnetic heating device for cycle heat conduction heating at a magnetic field intensity of 220 mT for 10 min to obtain extract R2;

[0200] (5) Add 5 g of crystal sugar into the extract R2, heat to 90° C. to completely dissolve, and weigh to obtain a medicinal and edible extract (B);

[0201] (6) placing the bird's nest (A) and the edible and medicinal extract (B) into a glass bottle according to certain specifications and sealing the bottle;

[0202] (7) Sterilization, 110°C, 20 min;

[0203] (8) Finished product.

[0204] Take the product prepared in Comparative Example 2 and Example 1 as the control group, and perform sensory preference scoring and color difference measurement on the finished product. The sensory preference evaluation standard is shown in Table 2. The test was conducted 5 times in parallel, and the average value was taken. The results are shown in Table 3. The product color diagram is shown in Figure 1 .

[0205] Table 2 Evaluation criteria for color preference

[0206]

[0207] Table 3 Decolorization process

[0208] Color preference (score) Total color difference (d*E) Example 1 9 110 Comparative Example 2 6 160

[0209] Depend on Figure 1 As shown in Table 3, the sensory effect of the bird's nest product is not good because the comparative example 2 does not undergo double decolorization, while the control sample (Example 1) has a higher color preference score and a lower total color difference. This indicates that the use of double decolorization technology can reduce the coloring power of the product and give the product a good sensory experience.

[0210] Comparative Example 3

[0211] The bird's nest formula product of this embodiment includes two parts: bird's nest (A) and medicine and food extract (B).

[0212] Bird's nest (A) includes the following raw materials in parts by weight: 5 g of bird's nest, and the corresponding preparation method is as follows:

[0213] The pure bird's nest was soaked for 2 hours, and then black spots, bird's hair, eggshell and other impurities were removed by picking, washing, drying and drying to obtain the bird's nest (A).

[0214] The edible and medicinal extract (B) comprises the following raw materials in parts by weight: 1 gram of ginseng, 5 grams of Cistanche deserticola, 5 grams of Dendrobium officinale, 5 grams of fresh Imperata root, 5 grams of Poria cocos, 5 grams of Eucommia male flower, 5 grams of Cordyceps militaris, and 5 grams of crystal sugar.

[0215] The preparation method of the bird's nest formula product is as follows:

[0216] (1) Mix the above Cordyceps militaris, ginseng, Cistanche deserticola, Dendrobium officinale, Poria cocos, Eucommia ulmoides male flower, and fresh Imperata cylindrica root, add 50 times of purified water, and stir and mix for 10 minutes;

[0217] (2) Using low-temperature circulation extraction technology, wait for the circulation extraction tank to be heated to 70°C and start extraction for 40 minutes;

[0218] (3) After the extraction is completed, the extract R1 is obtained by filtering through 300 mesh;

[0219] (4) Using double decolorization technology, 0.1 w / v of activated carbon was added to the extract R1, stirred and dissolved, and then passed through a 0.22 μm water film to obtain a prepared extract R2;

[0220] (5) Add 5 g of crystal sugar into the extract R2, heat to 90° C. to completely dissolve, and weigh to obtain a medicinal and edible extract (B);

[0221] (6) Place the bird's nest (A) and the edible and medicinal extract (B) into a PP bowl according to certain specifications and seal the bowl;

[0222] (7) Sterilization, 110°C, 20 min;

[0223] (8) Finished product.

[0224] The product of comparative example 3 was taken as the control group, and the effective ingredients of the finished product were tested. Five groups were tested in parallel, and the average value was taken. The results are shown in Table 4.

[0225] Table 4 Effect of encapsulation process on the content of active ingredients

[0226] Polysaccharides Saponin Polyphenols protein Example 1 20.1g / 100g 255mg / kg 1.5g / 100g 2.0g / 100g Comparative Example 3 15.7g / 100g 200mg / kg 1.2g / 100g 1.9g / 100g

[0227] It can be seen from Table 4 that the content of effective ingredients in the control sample (Example 1) is significantly higher than that in Example 3, indicating that the low-high frequency electromagnetic field cycle assisted packaging technology can effectively avoid the decomposition and loss of effective ingredients during the reheating process and improve the efficacy of the bird's nest product.

[0228] Comparative Example 4

[0229] The process for preparing the product in this example is the same as that in Example 1, except that the heavy raw materials included in the medicinal and edible extract (B) are different. The formula of this example is as follows:

[0230] 5 grams of bird's nest, 1 gram of ginseng, 5 grams of Cistanche deserticola, 5 grams of cinnamon, 5 grams of Poria cocos, 5 grams of fresh Imperata root, 5 grams of Eucommia ulmoides flower, 5 grams of raspberry, and 5 grams of rock sugar.

[0231] It is known that Cordyceps militaris and raspberry are both effective for impotence and spermatorrhea, and the effects are the same; Dendrobium officinale and cinnamon are both effective for yin deficiency and hyperactivity of fire, and the effects are the same.

[0232] The product of comparative example 4 was taken, and Example 1 was used as a control group to conduct a finished product efficacy experiment. In this experiment, the serum cyclic nucleotide levels of mice with kidney yin deficiency were measured. Because cAMP and cGMP are a pair of mutually antagonistic and mutually restrictive substances, they have a two-way control and regulatory effect on the stable state of cell function. Studies have shown that the increase of cAMP, cAMP / cGMP, and the decrease of cGMP indicate that the body is in a state of yin deficiency.

[0233] Efficacy evaluation 1

[0234] Experimental Materials and Methods

[0235] (1) Animal husbandry and grouping

[0236] Forty male Kunming mice (6 weeks old, 18 ± 3 g) were purchased from Sibeifu (Suzhou) Biotechnology Co., Ltd. (production license number SCXK (Su) 2022-0006) and housed in the New Animal Center of China Pharmaceutical University with a 12 h light-dark cycle, room temperature of 25 ± 0.5 °C, and relative humidity of 45 ± 5%. The experimental animals were allowed to eat and drink freely.

[0237] After 7 days of adaptive feeding, the mice were randomly divided into 6 groups, namely, blank group, model group, drug administration group 1 (41.50 g / kg group), and drug administration group 2 (41.50 g / kg group), with 10 mice in each group.

[0238] (2) Establishment of animal model of kidney yin deficiency and drug administration

[0239] The mice in the medication group 1 were gavaged with the homemade men's bird's nest product (the product prepared in Example 1), the mice in the medication group 2 were gavaged with the homemade synergistic formula product (the product prepared in Comparative Example 4), and the control group and the model group were gavaged with an equal amount of solvent; two weeks after gavage administration, all groups were subcutaneously injected with hydrocortisone (25.00 mg / kg) for 14 consecutive days to prepare a kidney yin deficiency model, and at the same time, the medication group 1 and the medication group 2 were gavaged with corresponding drugs.

[0240] (3) Determination of mouse serum indicators

[0241] After continuous modeling and drug administration for 14 days, the eyeballs were removed to collect blood and the mice were killed. The whole blood was placed at room temperature for 30 minutes and then centrifuged at 3500r / min for 10 minutes. The supernatant was collected and frozen at -80℃ for later use to detect cAMP, cGMP levels and reproductive hormones T and E2.

[0242] (4) Experimental results

[0243] cAMP and cGMP are important regulatory substances involved in energy metabolism in cells. Studies have shown that the cAMP level in the serum of patients with kidney yin deficiency increases, the cGMP level decreases, and the cAMP / cGMP ratio also increases significantly. As shown in Figure 2, compared with the blank group mice, the cAMP in the serum of the model group mice increased significantly, the cGMP decreased significantly, and the cAMP / cGMP increased significantly, indicating that the model group mice were in a state of kidney yin deficiency. Compared with the blank group, the cAMP, cGMP, and cAMP / cGMP indicators in the serum of the mice in the administration group 1 were closer than those in the administration group 2. It is shown that under the same process conditions, the formula of Example 1 is more effective than Comparative Example 4.

[0244] Efficacy evaluation 2

[0245] Experimental Materials and Methods

[0246] (1) Animal husbandry and grouping

[0247] Sixty Kunming male mice (6 weeks old, 18 ± 3 g) were purchased from Sibeifu (Suzhou) Biotechnology Co., Ltd. (production license number SCXK (Su) 2022-0006) and housed in the New Animal Center of China Pharmaceutical University with a 12 h light-dark cycle, room temperature of 25 ± 0.5 °C, and relative humidity of 45 ± 5%. The experimental animals were allowed to eat and drink freely.

[0248] After 7 days of adaptive feeding, the mice were randomly divided into 6 groups, namely, blank group, model group, positive group, and drug-treated group (10.38 g / kg group, 20.75 g / kg group, and 41.50 g / kg group), with 10 mice in each group.

[0249] (2) Establishment of animal model of kidney yin deficiency and drug administration

[0250] The mice in the drug-dosing groups were gavaged with different amounts of the homemade men's bird's nest product (the product prepared in Example 1), the positive group was gavaged with 78.80 mg / kg / d of Liuwei Dihuang Capsules, a medicine for kidney yin deficiency, and the control group and the model group were gavaged with an equal amount of solvent; two weeks after gavage, all groups were subcutaneously injected with hydrocortisone (25.00 mg / kg) for 14 consecutive days to prepare a kidney yin deficiency model, and the drug-dosing group and the positive group were gavaged with corresponding drugs.

[0251] (3) General morphological evaluation

[0252] According to the diagnostic criteria for kidney yin deficiency syndrome in the "Reference Standards for Differentiation of Deficiency Syndrome in Traditional Chinese Medicine", the behavioral changes of mice in the kidney yin deficiency group, such as mental state, activity frequency, hair color, water intake, food intake, defecation, and body weight, were observed. It was determined whether the different syndrome phenomena of mice were consistent with the clinical manifestations, which was used as the basis for judging whether the model was successfully established.

[0253] (4) Determination of mouse serum indicators

[0254] After continuous modeling and drug administration for 14 days, the eyeballs were removed to collect blood and the mice were killed. The whole blood was placed at room temperature for 30 minutes and then centrifuged at 3500r / min for 10 minutes. The supernatant was collected and frozen at -80℃ for later use to detect cAMP, cGMP levels and reproductive hormones T and E2.

[0255] (5) Observation of mouse semen quality

[0256] The stripped epididymis was transferred to PBS liquid, and the tail of the epididymis was cut into pieces with ophthalmic forceps, and then placed in a 37°C water bath. After 10 minutes, part of the liquid was absorbed and evenly applied to a clean slide. After natural drying, the film was first fixed with ethanol to prevent it from being washed away by water. Then it was fixed with anhydrous ethanol for 10 minutes; the ethanol was discarded at an angle, and 150 μL of 0.1% crystal violet solution was added to each slide, and dyed for 1 minute; washed with water for 1 minute, and then washed again with running water for 10-20 seconds; dried naturally, and the sperm cell morphology was observed under a microscope.

[0257] (6) Pathological tissue analysis of mouse kidney and testis

[0258] Kidneys and testicles of mice were removed, rinsed with saline, and fixed in 4% paraformaldehyde. Hematoxylin-eosin staining was used to observe the pathological changes of kidneys and testicles.

[0259] (7) Statistical analysis

[0260] Data were entered and sorted using Microsoft Excel, and statistical analysis was performed using GraphPad Prism 9.0 software, including t-test and analysis of variance. P < 0.05 was considered statistically significant.

[0261] Experimental Results

[0262] (1) Effects of bird's nest formula products on general morphology, body weight, body temperature, water intake, and food intake of mice with kidney yin deficiency

[0263] Glucocorticoids at physiological doses have the meaning of "less fire, more qi". When using large doses of hormones, this "pure yang" medicine is prone to "yang prevailing and consuming yin", affecting the restraint of yin essence and failing to play its nourishing role, resulting in kidney yin deficiency or yin deficiency and hyperactivity of fire, and symptoms of hyperadrenocortical hyperfunction such as excitement, restlessness, hot flashes, night sweats, etc. Long-term use of large doses of glucocorticoids can cause adrenal cortical hypofunction and even glandular atrophy.

[0264] Adrenocortical hormone modeling is one of the currently recognized methods for modeling kidney yin deficiency. In this study, two weeks after the injection of adrenocortical hormone hydrocortisone injection, the mice in the model group had increased spontaneous activity, sparse and messy fur, dry and hard stools, weight loss, reduced food intake, increased water intake, and active reactions. The above symptoms are similar to the manifestations of yin deficiency (deficient heat) in traditional Chinese medicine.

[0265] Figure 3 and Figure 4 The relevant experimental results of each group were displayed, indicating that compared with the mice in the model group, after being given the bird's nest formula product, the mice's fur regained its luster, and their stool, body weight, food intake, and water intake gradually returned to normal levels, and their reactions were normal.

[0266] (2) Effects of bird's nest formula products on serum cyclic nucleotide levels of cAMP and cGMP in mice with kidney yin deficiency

[0267] cAMP and cGMP are important regulatory substances involved in energy metabolism in cells. Studies have shown that the serum cAMP level in patients with kidney yin deficiency is increased, the cGMP level is decreased, and the cAMP / cGMP ratio is also significantly increased.

[0268] The cAMP and cGMP levels of mice in each group were determined using the above method. Figure 5Figures AC show the relevant results, indicating that compared with the blank group mice, the cAMP in the serum of the model group mice was significantly increased (P<0.001), the cGMP was significantly decreased (P<0.01), and the cAMP / cGMP was significantly increased (P<0.001), indicating that the model group mice were in a state of kidney yin deficiency. Compared with the model group mice, the cAMP in the serum of the positive group mice given Liuwei Dihuang Capsule was significantly decreased (P<0.001), the cGMP was significantly increased (P<0.01), and the cAMP / cGMP content was significantly decreased (P<0.001); after being given the men's bird's nest formula product, the cAMP in the serum of the 10.38g / kg group mice was significantly decreased (P<0.01), and the cAMP / cGMP content was significantly decreased (P<0.01); the cAMP in the serum of the 20.75g / kg group mice was significantly decreased (P<0.001), the cGMP was significantly increased (P<0.01), and the cAMP / cGMP content was significantly decreased (P<0.001); the cAMP in the serum of the 41.50g / kg group mice was significantly decreased (P<0.001), the cGMP was significantly increased (P<0.01), and the cAMP / cGMP content was significantly decreased (P<0.001).

[0269] (3) Effects of bird's nest formula products on reproductive hormones T and E2 in mice with kidney yin deficiency

[0270] The above method was used to measure the levels of reproductive hormones T and E2 in each group of mice. Figure 6 Figures AC show the relevant results, indicating that compared with the blank group, the E2 in the serum of the mice in the model group was significantly increased (P<0.001), T was significantly decreased (P<0.001), and T / E2 was significantly decreased (P<0.001), indicating that the mice in the model group were in a state of kidney yin deficiency. Compared with the model group mice, the serum E2 of the positive group mice given Liuwei Dihuang Capsule was significantly decreased (P<0.001), T was significantly increased (P<0.001), and the T / E2 content was significantly increased (P<0.001); after giving the men's bird's nest formula product, the serum E2 of the 10.38g / kg group mice was significantly decreased (P<0.01); the serum E2 of the 20.75g / kg group mice was significantly decreased (P<0.001), and the T / E2 content was significantly increased (P<0.01); the serum E2 of the 41.50g / kg group mice was significantly decreased (P<0.001), T was significantly increased (P<0.01), and the T / E2 content was significantly increased (P<0.001).

[0271] (4) Effects of bird's nest formula products on renal tissue pathology in mice with kidney yin deficiency

[0272] The above method was used to determine the effect of bird's nest formula products on kidney tissue of mice with kidney yin deficiency. The results are as follows: Figure 7As shown, the renal tissue structure of the mice in the blank group was relatively normal, the renal tubules were neatly arranged, the renal tubular epithelial cells were not degenerated, and there was no obvious inflammatory cell infiltration in the tissue; the renal tissue structure of the mice in the model group was abnormal, a large number of renal tubular structures were disordered, the renal tubular epithelial cells were edematous and degenerated, and inflammatory cell infiltration and renal interstitial edema were visible. After administering the bird's nest formula product, compared with the mice in the model group, the degree of disorder in the renal tubular structure of the mice in the positive group and the mice in each drug-treated group was significantly reduced, the number of epithelial cell degeneration and edema and the number of inflammatory cells were reduced, and the degree of damage was improved.

[0273] (5) Effect of bird's nest formula products on semen quality of mice with kidney yin deficiency

[0274] The kidney is the foundation of the body. It stores essence and controls reproduction. The production of reproductive essence depends on the normal function of the kidney. If the kidney qi is sufficient, the reproductive essence can be produced, grown and stored. Male kidney yin deficiency type oligoasthenospermia is characterized by poor sperm motility and number.

[0275] The inventors used the above method to measure the effect of bird's nest formula products on mouse sperm, and the results are as follows: Figure 8 As shown, the blank group mice had high sperm density and strong motility; the model group mice had significantly lower sperm relative density and poor sperm motility. After being given the bird's nest formula product, the relative sperm density of the mice in the drug-treated group increased significantly compared with the model group, and the sperm motility tended to increase.

[0276] (6) Effects of bird's nest formula products on testicular pathological characteristics of mice with kidney yin deficiency

[0277] The inventors used the above method to determine the effect of bird's nest formula products on the testicular pathological characteristics of kidney yin deficiency mice. The results are as follows: Fig. 9 As shown, the blank group mice had regular arrangement of spermatogenic cells in the seminiferous tubules, continuous and intact basement membranes, and evenly distributed interstitial cells; the model group mice had varying degrees of hollow tubular seminiferous tubules, damaged and thinned walls, basement membrane continuity damage, disordered arrangement of germ cells, reduced number of layers, unclear structure, and uneven distribution of interstitial cells. After administering the bird's nest formula product, compared with the model group mice, the hollow seminiferous tubules of the testicular seminiferous tubules of the drug-treated group mice were improved, the basement membrane was restored to integrity, the distribution of interstitial cells was restored to uniformity, the germ cells were arranged in an orderly manner, the number of layers increased, and the damaged testicular structure was restored.

[0278] The present invention replicates an experimental animal model of kidney yin deficiency caused by exogenous glucocorticoid by giving mice a large dose of hydrocortisone in a short period of time. The results show that 2 days after the injection of hydrocortisone, the mice begin to gradually show increased spontaneous activity, sparse and messy fur, dry and hard stools, weight loss, reduced food intake, increased water intake, active reactions, etc., which are basically consistent with the symptoms of human yin deficiency.

[0279] T and E2 play an important role in growth, development and reproduction. According to traditional Chinese medicine, the kidney stores essence and controls reproduction, which is closely related to growth and development. Only when the kidney yin and kidney yang are balanced can normal reproductive function be guaranteed. T and E2 are also closely related to kidney yin. This study confirmed that the serum T of mice in the kidney yin deficiency model group was significantly reduced, E2 was significantly increased, and T / E2 was significantly reduced; after giving the men's bird's nest product, the serum T of the mice was significantly increased, E2 was significantly reduced, and T / E2 was significantly increased, indicating that the men's bird's nest formula product can improve the hydrocortisone-induced mouse kidney yin deficiency model.

[0280] cAMP and cGMP are a pair of substances that antagonize and restrict each other, and have a two-way control and regulation effect on the stability of cell functions. Studies have shown that the increase of cAMP and cAMP / cGMP and the decrease of cGMP indicate that the body is in a state of Yin deficiency. The experimental results showed that the cAMP and cAMP / cGMP of the model group mice were significantly higher than those of the blank group mice, and cGMP was significantly lower, indicating that the mice were in a state of Yin deficiency. After giving bird's nest, cAMP and cAMP / cGMP were downregulated and cGMP increased, indicating that the bird's nest formula product has a corrective effect on the cAMP and cGMP disorder of hydrocortisone-induced kidney Yin deficiency mice.

[0281] In summary, from the analysis of the general morphology, serum indicators, kidney, testicular pathological tissue sections and sperm morphology of mice, the bird's nest formula product has the effect of alleviating symptoms and regulating disorders in the kidney yin deficiency model mice, and the comprehensive effect is better than that of Liuwei Dihuang Capsule. In addition, this formula also has a good effect on testicles, sperm, etc., and is very suitable for male consumer groups.

[0282] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", "some implementation schemes" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, unless they are contradictory.

[0283] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A composition, characterized in that include: A first material, wherein the raw material of the first material includes bird's nest; The second material comprises the following raw materials: ginseng, Cistanche deserticola, Dendrobium candidum, fresh Imperata root, Poria cocos, Eucommia ulmoides male flower, crystal sugar and Cordyceps militaris.

2. The composition according to claim 1, characterized in that The raw materials of the second material further include cyclodextrin.

3. The composition according to claim 1 or 2, characterized in that The mass ratio of the first material to the second material is (5-15):(40-140); Optionally, in the second material, the mass ratio of ginseng, Cistanche deserticola, Dendrobium officinale, fresh Imperata root, Poria cocos, Eucommia ulmoides male flower, crystal sugar, Cordyceps militaris, and cyclodextrin is (1-3): (5-15): (5-20), (5-45): (5-15): (5-10): (5-15): (5-15): (0.5-1.0).

4. The composition according to claim 1, characterized in that The first material is a liquid or a dry solid; Optionally, the second material is a liquid; Optionally, the first material and the second material are placed separately or mixed.

5. Use of the composition according to any one of claims 1 to 4 in preparing a bird's nest formula product for improving kidney yin deficiency function.

6. A bird's nest formula product, characterized in that: The invention comprises the composition according to any one of claims 1 to 4.

7. The method for preparing the bird's nest formula product according to claim 6, characterized in that: include: S1: pre-treating the bird's nest raw material to obtain the first material; S2: Mix the raw materials of ginseng, Cistanche deserticola, Dendrobium officinale, fresh Imperata root, Poria cocos, Eucommia ulmoides male flower, and Cordyceps militaris, and use low-temperature circulation extraction technology to obtain the medicinal and edible extract R1; S3: performing double decolorization treatment on the food-medicine-susceptible extract R1 to obtain the food-medicine-susceptible extract R2; S4: adding cyclodextrin to the food-medicine homologous extract R2, and using low-high frequency electromagnetic field circulation-assisted encapsulation technology to encapsulate the active ingredients in the food-medicine homologous mixed solution R2, so as to obtain the food-medicine homologous extract R3; S5: Mix the first material with the food and medicine extract R3 to obtain the bird's nest formula product.

8. The preparation method according to claim 7, characterized in that: In step S1, the pre-processing includes: Remove impurities and dry the bird's nest raw materials; Optionally, in step S2, the extraction temperature using the low-temperature cycle extraction technology is 50-70°C, and the extraction time is 40-50min; Optionally, in step S3, the double decolorization treatment includes decolorizing the edible and medicinal extract R1 using activated carbon and water film.

9. The preparation method according to claim 7, characterized in that: In step S4, the mass ratio of the amount of the cyclodextrin added to the total weight of the raw materials mixed in step S1 is (0.5-1.0): (31-123); Optionally, the parameters of the low-high frequency electromagnetic field cycle-assisted wrapping technology are: magnetic field intensity 200-300 mT, time 10-20 min; Optionally, the preparation method further comprises: before performing step S5, adding crystal sugar to the medicine-food homologous extract R3 obtained in step S4; Optionally, in step S5, when the first material is solid, when the first material is mixed with the medicinal and edible extract R3, the mass volume ratio is (5-15): (100-200) g / ml; Optionally, step S5 further includes sterilizing the mixture of the first material and the food and medicine extract R3.

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